Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0285770 | XP_003595397.1 | 100.000 | 78 | 0 | 0 | 1 | 78 | 1 | 78 | 4.38e-49 | 159 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0285770 | A2Q3Y5 | 100.000 | 78 | 0 | 0 | 1 | 78 | 1 | 78 | 2.09e-49 | 159 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0089980 | Msa0285770 | 0.804436 | 2.162603e-49 | -8.615850e-47 |
| Msa0090010 | Msa0285770 | 0.804608 | 1.991040e-49 | -8.615850e-47 |
| Msa0138360 | Msa0285770 | 0.803860 | 2.850073e-49 | -8.615850e-47 |
| Msa0141400 | Msa0285770 | 0.811678 | 6.209318e-51 | -8.615850e-47 |
| Msa0147980 | Msa0285770 | 0.816153 | 6.403384e-52 | -8.615850e-47 |
| Msa0285770 | Msa0527190 | 0.805346 | 1.395288e-49 | -8.615850e-47 |
| Msa0285770 | Msa0615840 | 0.800028 | 1.746276e-48 | -8.615850e-47 |
| Msa0285770 | Msa0698100 | 0.807230 | 5.594609e-50 | -8.615850e-47 |
| Msa0285770 | Msa1371520 | 0.801051 | 1.080381e-48 | -8.615850e-47 |
| Msa0285770 | Msa1393820 | 0.806343 | 8.614045e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0285770 | MtrunA17_Chr2g0302081 | 100.000 | 78 | 0 | 0 | 1 | 78 | 1 | 78 | 4.03e-53 | 159 |
| Msa0285770 | MtrunA17_Chr2g0302071 | 70.513 | 78 | 23 | 0 | 1 | 78 | 1 | 78 | 8.33e-35 | 113 |
| Msa0285770 | MtrunA17_Chr4g0069511 | 59.756 | 82 | 27 | 2 | 1 | 78 | 1 | 80 | 1.30e-25 | 90.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0285770 | AT3G05858.1 | 52.500 | 80 | 33 | 3 | 1 | 75 | 1 | 80 | 6.62e-16 | 66.2 |
| Msa0285770 | AT5G26620.1 | 45.333 | 75 | 30 | 1 | 1 | 75 | 1 | 64 | 1.05e-14 | 62.8 |
Find 15 sgRNAs with CRISPR-Local
Find 14 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGGAAACAATGATGAATAT+TGG | 0.265744 | 2_3:-25744699 | Msa0285770:CDS |
| AATAAATTCAAGAAGCAAAT+TGG | 0.275558 | 2_3:+25744539 | None:intergenic |
| TTCCTTATGCAATGTATTCA+AGG | 0.335158 | 2_3:-25744734 | Msa0285770:CDS |
| TGGAAACAATGATGAATATT+GGG | 0.371421 | 2_3:-25744698 | Msa0285770:CDS |
| TATTGGGAAGGTAGTGGAAG+TGG | 0.432591 | 2_3:-25744682 | Msa0285770:CDS |
| GGTTGTTGGGTTGCTGAGCC+TGG | 0.459748 | 2_3:-25744628 | Msa0285770:CDS |
| TGCCTTGAATACATTGCATA+AGG | 0.475361 | 2_3:+25744732 | None:intergenic |
| TGATGAAGATAGAGGTTGTT+GGG | 0.482176 | 2_3:-25744641 | Msa0285770:CDS |
| GATGAATATTGGGAAGGTAG+TGG | 0.519319 | 2_3:-25744688 | Msa0285770:CDS |
| TTCAAGGCATGTTTCTCAAG+TGG | 0.530306 | 2_3:-25744718 | Msa0285770:CDS |
| GTGATGAAGATAGAGGTTGT+TGG | 0.556565 | 2_3:-25744642 | Msa0285770:CDS |
| AACAATGATGAATATTGGGA+AGG | 0.569030 | 2_3:-25744694 | Msa0285770:CDS |
| TTGCTGAGCCTGGTATTGAT+AGG | 0.577391 | 2_3:-25744618 | Msa0285770:CDS |
| TTTGCAAGTGATGAAGATAG+AGG | 0.587569 | 2_3:-25744649 | Msa0285770:CDS |
| GAAGCTTTCCTATCAATACC+AGG | 0.623221 | 2_3:+25744610 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TGGAAACAATGATGAATATT+GGG | - | chr2_3:25744608-25744627 | Msa0285770:CDS | 25.0% |
| TTCCTTATGCAATGTATTCA+AGG | - | chr2_3:25744572-25744591 | Msa0285770:CDS | 30.0% | |
| GTGGAAACAATGATGAATAT+TGG | - | chr2_3:25744607-25744626 | Msa0285770:CDS | 30.0% | |
| AACAATGATGAATATTGGGA+AGG | - | chr2_3:25744612-25744631 | Msa0285770:CDS | 30.0% | |
| TGCCTTGAATACATTGCATA+AGG | + | chr2_3:25744577-25744596 | None:intergenic | 35.0% | |
| TTTGCAAGTGATGAAGATAG+AGG | - | chr2_3:25744657-25744676 | Msa0285770:CDS | 35.0% | |
| TGATGAAGATAGAGGTTGTT+GGG | - | chr2_3:25744665-25744684 | Msa0285770:CDS | 35.0% | |
| TTCAAGGCATGTTTCTCAAG+TGG | - | chr2_3:25744588-25744607 | Msa0285770:CDS | 40.0% | |
| GATGAATATTGGGAAGGTAG+TGG | - | chr2_3:25744618-25744637 | Msa0285770:CDS | 40.0% | |
| GTGATGAAGATAGAGGTTGT+TGG | - | chr2_3:25744664-25744683 | Msa0285770:CDS | 40.0% | |
| GAAGCTTTCCTATCAATACC+AGG | + | chr2_3:25744699-25744718 | None:intergenic | 40.0% | |
| !! | TATTGGGAAGGTAGTGGAAG+TGG | - | chr2_3:25744624-25744643 | Msa0285770:CDS | 45.0% |
| !! | TTGCTGAGCCTGGTATTGAT+AGG | - | chr2_3:25744688-25744707 | Msa0285770:CDS | 45.0% |
| ! | GGTTGTTGGGTTGCTGAGCC+TGG | - | chr2_3:25744678-25744697 | Msa0285770:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_3 | gene | 25744546 | 25744782 | 25744546 | ID=Msa0285770;Name=Msa0285770 |
| chr2_3 | mRNA | 25744546 | 25744782 | 25744546 | ID=Msa0285770-mRNA-1;Parent=Msa0285770;Name=Msa0285770-mRNA-1;_AED=0.26;_eAED=0.26;_QI=0|-1|0|1|-1|1|1|0|78 |
| chr2_3 | exon | 25744546 | 25744782 | 25744546 | ID=Msa0285770-mRNA-1:exon:9528;Parent=Msa0285770-mRNA-1 |
| chr2_3 | CDS | 25744546 | 25744782 | 25744546 | ID=Msa0285770-mRNA-1:cds;Parent=Msa0285770-mRNA-1 |
| Gene Sequence |
| Protein sequence |
