AlfalfaGEDB | Msa0138360

M. sativa cultivar zhongmu-4 / Msa0138360

KEGG: K15285
GO: GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0006810 GO:0008150 GO:0012505 GO:0015291 GO:0015297 GO:0015780 GO:0015931 GO:0022804 GO:0022857 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0090480 GO:1901264
PFAM: PF03151.19

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0138360	XP_013466230.1	99.710	345	1	0	1	345	1	345	0.0	693

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0138360	sp\|Q9LPU2\|URGT2_ARATH	79.883	343	69	0	2	344	5	347	0.0	559

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0138360	A0A072VF94	99.710	345	1	0	1	345	1	345	0.0	693

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000880	Msa0138360	0.869432	3.110007e-66	-8.615850e-47
Msa0000910	Msa0138360	0.826544	2.559714e-54	-8.615850e-47
Msa0001450	Msa0138360	0.831051	2.077590e-55	-8.615850e-47
Msa0001960	Msa0138360	0.810660	1.032230e-50	-8.615850e-47
Msa0002560	Msa0138360	0.805696	1.178439e-49	-8.615850e-47
Msa0002680	Msa0138360	0.902426	9.812798e-79	-8.615850e-47
Msa0002710	Msa0138360	0.872846	2.322442e-67	-8.615850e-47
Msa0003220	Msa0138360	0.860970	1.424626e-63	-8.615850e-47
Msa0003270	Msa0138360	0.836301	1.014048e-56	-8.615850e-47
Msa0003700	Msa0138360	0.823551	1.303088e-53	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0138360	MtrunA17_Chr1g0157501	99.710	345	1	0	1	345	1	345	0.0	693
Msa0138360	MtrunA17_Chr5g0393381	79.179	341	70	1	5	344	7	347	0.0	532
Msa0138360	MtrunA17_Chr8g0383381	51.692	325	145	3	12	335	14	327	2.46e-106	315
Msa0138360	MtrunA17_Chr3g0129401	50.156	321	147	5	2	316	6	319	2.53e-104	309
Msa0138360	MtrunA17_Chr1g0201531	52.665	319	140	5	2	316	6	317	1.54e-101	301
Msa0138360	MtrunA17_Chr4g0028931	36.446	332	201	2	4	335	3	324	2.57e-66	213
Msa0138360	MtrunA17_Chr6g0467391	39.649	285	170	1	14	298	13	295	2.68e-65	209
Msa0138360	MtrunA17_Chr6g0467371	39.649	285	170	1	14	298	13	295	2.68e-65	209
Msa0138360	MtrunA17_Chr2g0326791	39.298	285	171	1	14	298	13	295	3.05e-65	210
Msa0138360	MtrunA17_Chr3g0128951	39.068	279	168	1	30	308	29	305	1.11e-61	201

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0138360	AT1G21070.1	79.883	343	69	0	2	344	5	347	0.0	559
Msa0138360	AT1G76670.2	79.883	343	68	1	2	344	5	346	0.0	549
Msa0138360	AT1G76670.1	79.883	343	68	1	2	344	5	346	0.0	549
Msa0138360	AT5G42420.1	77.746	346	74	2	2	344	4	349	0.0	541
Msa0138360	AT4G09810.1	51.713	321	142	5	2	316	6	319	1.38e-107	318
Msa0138360	AT1G34020.2	51.792	307	137	4	16	316	18	319	7.12e-103	306
Msa0138360	AT1G34020.1	51.792	307	137	4	16	316	18	319	7.12e-103	306
Msa0138360	AT1G34020.3	51.792	307	137	4	16	316	18	319	7.12e-103	306
Msa0138360	AT4G39390.1	48.137	322	162	3	3	321	10	329	2.92e-99	297
Msa0138360	AT4G39390.2	48.137	322	162	3	3	321	10	329	2.92e-99	297

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Find 128 sgRNAs with CRISPR-Local

Find 219 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTAAGCATGATTCCGG+TGG	0.633913	1_3:-71184872	Msa0138360:CDS
AAAAGGAAAGAAAGTAAGTG+AGG	0.515205	1_3:+71186010	None:intergenic
AAAATTAAAATTGTTGGATA+AGG	0.330145	1_3:-71183343	Msa0138360:three_prime_UTR
AAACAGCTTATGTCCAACAA+TGG	0.647240	1_3:-71185690	Msa0138360:CDS
AAAGAAATCGACCTCAGATC+TGG	0.492639	1_3:-71185919	Msa0138360:five_prime_UTR
AAATTAGGCTATTGAAGGAA+GGG	0.573519	1_3:-71183445	Msa0138360:CDS
AACATTATGTGTTCTGACTT+TGG	0.411202	1_3:-71183624	Msa0138360:CDS
AACCAAATAAGCTCCCACAA+AGG	0.703411	1_3:+71185441	None:intergenic
AACGCAACTTGAATCAATTA+TGG	0.358686	1_3:-71185799	Msa0138360:exon
AAGAAAGTAAGTGAGGAGGG+AGG	0.582082	1_3:+71186017	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAGGAAAGAAAGTAAGTG+AGG	+	chr1_3:71183347-71183366	None:intergenic	30.0%
AACGCAACTTGAATCAATTA+TGG	-	chr1_3:71183555-71183574	Msa0138360:CDS	30.0%
ATTAGCCATGATAATTCCAA+CGG	+	chr1_3:71183644-71183663	None:intergenic	30.0%
AATGGTTATGCTTTCACTTT+CGG	-	chr1_3:71183682-71183701	Msa0138360:CDS	30.0%
TCATTACTACAGAATCTACA+GGG	+	chr1_3:71184076-71184095	None:intergenic	30.0%
TATCATAGACCACCATATTT+CGG	+	chr1_3:71184202-71184221	None:intergenic	30.0%
ATCAAAACTAAGCATGATTC+CGG	-	chr1_3:71184479-71184498	Msa0138360:intron	30.0%
TCTTTACAGCAAATTGTAAG+TGG	-	chr1_3:71184663-71184682	Msa0138360:intron	30.0%
AAACAGACTTCATTGTTAAC+AGG	-	chr1_3:71185009-71185028	Msa0138360:intron	30.0%
AACAGACTTCATTGTTAACA+GGG	-	chr1_3:71185010-71185029	Msa0138360:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_3	gene	71183041	71186335	71183041	ID=Msa0138360;Name=Msa0138360
chr1_3	mRNA	71183041	71186335	71183041	ID=Msa0138360-mRNA-1;Parent=Msa0138360;Name=Msa0138360-mRNA-1;_AED=0.06;_eAED=0.06;_QI=533\|1\|1\|1\|1\|1\|5\|353\|345
chr1_3	exon	71183041	71183753	71183041	ID=Msa0138360-mRNA-1:exon:24738;Parent=Msa0138360-mRNA-1
chr1_3	exon	71183870	71184031	71183870	ID=Msa0138360-mRNA-1:exon:24737;Parent=Msa0138360-mRNA-1
chr1_3	exon	71184699	71184899	71184699	ID=Msa0138360-mRNA-1:exon:24736;Parent=Msa0138360-mRNA-1
chr1_3	exon	71185368	71185552	71185368	ID=Msa0138360-mRNA-1:exon:24735;Parent=Msa0138360-mRNA-1
chr1_3	exon	71185673	71186335	71185673	ID=Msa0138360-mRNA-1:exon:24734;Parent=Msa0138360-mRNA-1
chr1_3	five_prime_UTR	71185803	71186335	71185803	ID=Msa0138360-mRNA-1:five_prime_utr;Parent=Msa0138360-mRNA-1
chr1_3	CDS	71185673	71185802	71185673	ID=Msa0138360-mRNA-1:cds;Parent=Msa0138360-mRNA-1
chr1_3	CDS	71185368	71185552	71185368	ID=Msa0138360-mRNA-1:cds;Parent=Msa0138360-mRNA-1
chr1_3	CDS	71184699	71184899	71184699	ID=Msa0138360-mRNA-1:cds;Parent=Msa0138360-mRNA-1
chr1_3	CDS	71183870	71184031	71183870	ID=Msa0138360-mRNA-1:cds;Parent=Msa0138360-mRNA-1
chr1_3	CDS	71183394	71183753	71183394	ID=Msa0138360-mRNA-1:cds;Parent=Msa0138360-mRNA-1
chr1_3	three_prime_UTR	71183041	71183393	71183041	ID=Msa0138360-mRNA-1:three_prime_utr;Parent=Msa0138360-mRNA-1

Gene Sequence

>Msa0138360

ATGGAGAGTGAAAAGAAATCGTCAGCAATATCGAACTTTGGAGCATGGGGTATGAACGTTGTGAGTTCCGTTGGAATTATCATGGCTAATAAACAGCTTATGTCCAACAATGGTTATGCTTTCACTTTCGCGACTACTTTGACTGGGTTTCACTTTGCTGTAACTGCTCTTGTCGGACTCGTCTCAAATGCTACCGGTTATTCTGCTTCTAAGCATGTTCCTTTGTGGGAGCTTATTTGGTTCTCTGCTGTTGCCAATATATCTATCACCGGGATGAACTTAAGCCTCATGCTCAATTCCGTTGGATTCTACCAAATATCAAAACTAAGCATGATTCCGGTGGTTTGTGTGATGGAATGGATCCTTCACAGCAAACACTACTCAAGGGAAGTGAAAGTGTCGGTCATGGTTGTGGTTATTGGTGTTGGTGTTTGCACTGTAACTGATGTAAATGTTAACTTCAAAGGTTTTGTGTGCGCCTGTGTAGCAGTTGTATCATCATCTTTACAGCAAATTTCAATCGGTTCTCTACAAAAGAAATATTCGATTGGATCTTTTGAACTGCTGAGTAAGACTGCTCCTATTCAAGCTCTCTCTCTTATTATTCTTGGTCCATTTGTTGATTACTATCTCAGTGGCAAGTTGATAACCAACTTTAAGATGTCTTCTGGTGCCATTGTTTTCATTCTTCTTTCATGCACCTTAGCTGTGTTTTGTAATGTAAGCCAGTACCTCTGCATTGGGCGCTTCTCAGCAGTTTCCTTCCAGGTTTTAGGCCACATGAAAACATTATGTGTTCTGACTTTGGGGTGGCTGTTATTCGATTCAGAGCTCACTTTCAAGAACATCATGGGAATGGTTCTTGCAGTTGTCGGCATGGTAATTTATAGTTGGGCTGTGGAGCTTGAAAAGCAGCCAAATGCGAAGACTCTCCCTCATTCGAAAAACAGCCTAACAGAAGAAGAAATTAGGCTATTGAAGGAAGGGGTAGAAAATAGTCCGTTGAAAGATATTGAACTTGGTCAGGCTAAAGTTTAG

Protein sequence

>Msa0138360

MESEKKSSAISNFGAWGMNVVSSVGIIMANKQLMSNNGYAFTFATTLTGFHFAVTALVGLVSNATGYSASKHVPLWELIWFSAVANISITGMNLSLMLNSVGFYQISKLSMIPVVCVMEWILHSKHYSREVKVSVMVVVIGVGVCTVTDVNVNFKGFVCACVAVVSSSLQQISIGSLQKKYSIGSFELLSKTAPIQALSLIILGPFVDYYLSGKLITNFKMSSGAIVFILLSCTLAVFCNVSQYLCIGRFSAVSFQVLGHMKTLCVLTLGWLLFDSELTFKNIMGMVLAVVGMVIYSWAVELEKQPNAKTLPHSKNSLTEEEIRLLKEGVENSPLKDIELGQAKV*