AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1342430


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1342430 MtrunA17_Chr4g0046991 98.148 108 1 1 1 107 11 118 1.75e-72 212
Msa1342430 MtrunA17_Chr2g0282521 67.105 76 22 2 33 107 36 109 7.48e-29 101
Msa1342430 MtrunA17_Chr8g0360361 73.469 49 13 0 59 107 62 110 3.57e-24 90.5
Msa1342430 MtrunA17_Chr2g0317041 68.750 48 15 0 60 107 97 144 3.29e-20 79.7
Msa1342430 MtrunA17_Chr8g0334671 55.422 83 30 2 27 107 155 232 6.39e-20 82.0
Msa1342430 MtrunA17_Chr1g0175031 62.264 53 20 0 55 107 67 119 1.81e-18 74.7
Msa1342430 MtrunA17_Chr7g0258471 65.306 49 17 0 59 107 139 187 1.95e-18 78.6
Msa1342430 MtrunA17_Chr7g0231911 67.391 46 15 0 62 107 149 194 4.15e-17 74.3
Msa1342430 MtrunA17_Chr3g0122411 50.000 64 32 0 44 107 147 210 4.97e-17 74.7
Msa1342430 MtrunA17_Chr2g0302791 52.308 65 27 1 47 107 120 184 7.56e-17 74.3
Msa1342430 MtrunA17_Chr7g0270541 55.738 61 27 0 47 107 130 190 8.48e-17 74.3
Msa1342430 MtrunA17_Chr1g0194121 55.000 60 27 0 48 107 133 192 1.42e-16 73.6
Msa1342430 MtrunA17_Chr3g0132091 53.226 62 29 0 46 107 57 118 1.46e-16 73.2
Msa1342430 MtrunA17_Chr7g0224821 52.459 61 27 1 47 107 64 122 1.52e-16 73.2
Msa1342430 MtrunA17_Chr5g0420051 50.820 61 27 1 50 107 166 226 1.99e-16 73.2
Msa1342430 MtrunA17_Chr8g0377421 48.276 87 37 2 29 107 91 177 5.79e-16 71.2
Msa1342430 MtrunA17_Chr8g0377401 61.224 49 19 0 59 107 159 207 7.45e-16 70.5
Msa1342430 MtrunA17_Chr1g0162871 64.583 48 17 0 60 107 102 149 7.63e-16 71.6
Msa1342430 MtrunA17_Chr4g0061651 63.043 46 17 0 62 107 123 168 1.25e-15 70.9
Msa1342430 MtrunA17_Chr8g0377431 61.702 47 18 0 60 106 149 195 5.96e-15 67.4
Msa1342430 MtrunA17_Chr2g0308321 60.870 46 18 0 62 107 171 216 2.53e-14 67.4
Msa1342430 MtrunA17_Chr8g0386271 63.043 46 17 0 62 107 99 144 8.03e-14 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1342430 AT2G33810.1 62.500 64 21 1 44 107 39 99 1.44e-22 85.5
Msa1342430 AT1G02065.2 57.143 70 28 1 40 107 164 233 4.89e-20 81.6
Msa1342430 AT1G53160.2 59.677 62 22 1 46 107 41 99 1.74e-19 78.6
Msa1342430 AT1G53160.1 59.677 62 22 1 46 107 41 99 1.74e-19 78.6
Msa1342430 AT1G53160.3 59.677 62 22 1 46 107 41 99 1.89e-19 78.6
Msa1342430 AT3G60030.1 55.738 61 27 0 47 107 112 172 9.03e-19 80.5
Msa1342430 AT1G02065.1 57.971 69 27 1 41 107 165 233 1.93e-18 78.6
Msa1342430 AT3G15270.1 67.391 46 15 0 62 107 63 108 3.57e-18 75.5
Msa1342430 AT2G47070.1 68.085 47 15 0 61 107 105 151 4.39e-18 78.6
Msa1342430 AT1G27360.2 50.725 69 34 0 39 107 152 220 9.67e-18 77.0
Msa1342430 AT1G27360.1 50.725 69 34 0 39 107 152 220 9.67e-18 77.0
Msa1342430 AT1G27360.3 50.725 69 34 0 39 107 152 220 9.67e-18 77.0
Msa1342430 AT1G27360.4 50.725 69 34 0 39 107 152 220 9.67e-18 77.0
Msa1342430 AT1G27370.6 45.588 68 37 0 40 107 154 221 1.48e-17 76.3
Msa1342430 AT5G43270.3 45.882 85 41 1 28 107 130 214 1.66e-17 76.6
Msa1342430 AT5G43270.1 45.882 85 41 1 28 107 130 214 1.66e-17 76.6
Msa1342430 AT5G43270.2 45.882 85 41 1 28 107 130 214 1.66e-17 76.6
Msa1342430 AT1G27370.1 45.588 68 37 0 40 107 154 221 2.61e-17 75.9
Msa1342430 AT1G27370.5 45.588 68 37 0 40 107 154 221 2.61e-17 75.9
Msa1342430 AT1G27370.3 45.588 68 37 0 40 107 154 221 2.61e-17 75.9
Msa1342430 AT1G27370.2 45.588 68 37 0 40 107 154 221 2.61e-17 75.9
Msa1342430 AT1G27370.4 45.588 68 37 0 40 107 154 221 2.61e-17 75.9
Msa1342430 AT1G27370.7 45.588 68 37 0 40 107 154 221 2.61e-17 75.9
Msa1342430 AT1G69170.1 55.738 61 24 1 50 107 109 169 1.05e-16 74.3
Msa1342430 AT1G20980.1 64.583 48 17 0 60 107 118 165 5.40e-16 72.4
Msa1342430 AT5G50570.1 55.769 52 23 0 56 107 95 146 8.86e-16 71.6
Msa1342430 AT5G50570.2 55.769 52 23 0 56 107 95 146 8.86e-16 71.6
Msa1342430 AT5G50670.1 55.769 52 23 0 56 107 95 146 8.86e-16 71.6
Msa1342430 AT5G50670.2 55.769 52 23 0 56 107 95 146 8.86e-16 71.6
Msa1342430 AT1G76580.1 64.583 48 17 0 60 107 112 159 4.07e-15 70.1
Msa1342430 AT2G42200.1 60.417 48 19 0 60 107 72 119 1.04e-14 68.6
Msa1342430 AT1G76580.3 64.583 48 17 0 60 107 112 159 1.34e-14 68.6
Msa1342430 AT1G76580.2 64.583 48 17 0 60 107 112 159 1.39e-14 68.6
Msa1342430 AT3G57920.1 46.667 75 34 1 33 107 36 104 1.66e-12 62.4

Find 23 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAGGAAGAGGATGGTAGA+AGG 0.346008 tig0005576:-3444 Msa1342430:CDS
TGAGGAGGAAGAAGAGGAAG+AGG 0.391649 tig0005576:-3479 Msa1342430:CDS
GGAAGAAGAGGAAGAGGATA+CGG 0.438830 tig0005576:-3473 Msa1342430:CDS
GCACCTGCCGTACACATTGC+AGG 0.447145 tig0005576:-3265 Msa1342430:CDS
AAGAGGATGGTAGAAGGAAG+AGG 0.452598 tig0005576:-3438 Msa1342430:CDS
GCAATATCACCGGAGACATA+AGG 0.487344 tig0005576:-3308 Msa1342430:CDS
AATGTGTACGGCAGGTGCTT+TGG 0.492016 tig0005576:+3270 Msa1342430:intergenic
GGATCATGAGGAGGAAGAAG+AGG 0.499097 tig0005576:-3485 Msa1342430:CDS
AGAGGATGGTAGAAGGAAGA+GGG 0.504666 tig0005576:-3437 Msa1342430:CDS
ACATTGCAGGATTGCAGCAA+AGG 0.514685 tig0005576:-3252 Msa1342430:CDS
TATTCCACCTTGTTGTCAAG+TGG 0.526109 tig0005576:-3362 Msa1342430:CDS
ACTCACAGACCTTATGTCTC+CGG 0.562455 tig0005576:+3299 None:intergenic
CAATTCTCCACTTGACAACA+AGG 0.571704 tig0005576:+3355 None:intergenic
CTGCAATCCTGCAATGTGTA+CGG 0.578035 tig0005576:+3258 None:intergenic
TTGAAACTATGGAGACAAGA+AGG 0.594631 tig0005576:-3540 None:intergenic
AATAAGTTTGAAGTACAAAG+AGG 0.596272 tig0005576:-3506 Msa1342430:CDS
AATCCTGCAATGTGTACGGC+AGG 0.603274 tig0005576:+3262 Msa1342430:intergenic
ATGGAGACAAGAAGGTCAGA+GGG 0.623380 tig0005576:-3532 Msa1342430:CDS
GTGAAGCTAAGCAATATCAC+CGG 0.630226 tig0005576:-3318 Msa1342430:CDS
TATGGAGACAAGAAGGTCAG+AGG 0.634836 tig0005576:-3533 Msa1342430:CDS
GTACAAAGAGGATCATGAGG+AGG 0.636625 tig0005576:-3494 Msa1342430:CDS
GAAGTACAAAGAGGATCATG+AGG 0.643502 tig0005576:-3497 Msa1342430:CDS
TTCTCCACTTGACAACAAGG+TGG 0.724295 tig0005576:+3358 Msa1342420:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAGTTTGAAGTACAAAG+AGG - tig0005576:3260-3279 Msa1342430:CDS 25.0%
AAGAGAAGTTCCAAAAAAGC+TGG - tig0005576:3372-3391 Msa1342430:CDS 35.0%
!!! ATTTGAGCTTCCAGCTTTTT+TGG + tig0005576:3385-3404 Msa1342430:intergenic 35.0%
GAAGTACAAAGAGGATCATG+AGG - tig0005576:3269-3288 Msa1342430:CDS 40.0%
!! AGAGGATACGGATTTTGAAG+AGG - tig0005576:3305-3324 Msa1342430:CDS 40.0%
!! TACGGATTTTGAAGAGGAAG+AGG - tig0005576:3311-3330 Msa1342430:CDS 40.0%
!! GATTTTGAAGAGGAAGAGGA+TGG - tig0005576:3315-3334 Msa1342430:CDS 40.0%
! TATTCCACCTTGTTGTCAAG+TGG - tig0005576:3404-3423 Msa1342430:CDS 40.0%
CAATTCTCCACTTGACAACA+AGG + tig0005576:3414-3433 Msa1342430:intergenic 40.0%
GTGAAGCTAAGCAATATCAC+CGG - tig0005576:3448-3467 Msa1342430:CDS 40.0%
ATGGAGACAAGAAGGTCAGA+GGG - tig0005576:3234-3253 Msa1342430:CDS 45.0%
GTACAAAGAGGATCATGAGG+AGG - tig0005576:3272-3291 Msa1342430:CDS 45.0%
GGAAGAAGAGGAAGAGGATA+CGG - tig0005576:3293-3312 Msa1342430:CDS 45.0%
AAGAGGAAGAGGATGGTAGA+AGG - tig0005576:3322-3341 Msa1342430:CDS 45.0%
AAGAGGATGGTAGAAGGAAG+AGG - tig0005576:3328-3347 Msa1342430:CDS 45.0%
AGAGGATGGTAGAAGGAAGA+GGG - tig0005576:3329-3348 Msa1342430:CDS 45.0%
TTCTCCACTTGACAACAAGG+TGG + tig0005576:3411-3430 Msa1342430:intergenic 45.0%
GCAATATCACCGGAGACATA+AGG - tig0005576:3458-3477 Msa1342430:CDS 45.0%
ACTCACAGACCTTATGTCTC+CGG + tig0005576:3470-3489 Msa1342430:intergenic 45.0%
CTGCAATCCTGCAATGTGTA+CGG + tig0005576:3511-3530 Msa1342430:intergenic 45.0%
ACATTGCAGGATTGCAGCAA+AGG - tig0005576:3514-3533 Msa1342430:CDS 45.0%
GGATCATGAGGAGGAAGAAG+AGG - tig0005576:3281-3300 Msa1342430:CDS 50.0%
TGAGGAGGAAGAAGAGGAAG+AGG - tig0005576:3287-3306 Msa1342430:CDS 50.0%
AATGTGTACGGCAGGTGCTT+TGG + tig0005576:3499-3518 Msa1342430:intergenic 50.0%
AATCCTGCAATGTGTACGGC+AGG + tig0005576:3507-3526 Msa1342430:intergenic 50.0%
GCACCTGCCGTACACATTGC+AGG - tig0005576:3501-3520 Msa1342430:CDS 60.0%
Chromosome Type Strat End Strand Name
tig0005576 gene 3234 3554 3234 ID=Msa1342430;Name=Msa1342430
tig0005576 mRNA 3234 3554 3234 ID=Msa1342430-mRNA-1;Parent=Msa1342430;Name=Msa1342430-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|-1|0|1|-1|1|1|0|107
tig0005576 exon 3234 3554 3234 ID=Msa1342430-mRNA-1:exon:3597;Parent=Msa1342430-mRNA-1
tig0005576 CDS 3234 3554 3234 ID=Msa1342430-mRNA-1:cds;Parent=Msa1342430-mRNA-1
Gene Sequence

>Msa1342430

ATGGAGACAAGAAGGTCAGAGGGAAAAATAAGTTTGAAGTACAAAGAGGATCATGAGGAGGAAGAAGAGGAAGAGGATACGGATTTTGAAGAGGAAGAGGATGGTAGAAGGAAGAGGGTAGTGACAGATCTCTATAGCAAGAGAAGTTCCAAAAAAGCTGGAAGCTCAAATATTCCACCTTGTTGTCAAGTGGAGAATTGTGATGCTGATCTTAGTGAAGCTAAGCAATATCACCGGAGACATAAGGTCTGTGAGTATCATGCCAAAGCACCTGCCGTACACATTGCAGGATTGCAGCAAAGGTTTTGTCAGCAATGTAGC

Protein sequence

>Msa1342430

METRRSEGKISLKYKEDHEEEEEEEDTDFEEEEDGRRKRVVTDLYSKRSSKKAGSSNIPPCCQVENCDADLSEAKQYHRRHKVCEYHAKAPAVHIAGLQQRFCQQCS