AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0338330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0338330 MtrunA17_Chr4g0064791 65.909 176 46 4 84 251 29 198 2.90e-73 222
Msa0338330 MtrunA17_Chr3g0139081 47.742 310 88 12 4 250 23 321 3.11e-60 201
Msa0338330 MtrunA17_Chr4g0000581 39.689 257 108 9 30 250 91 336 5.74e-37 137
Msa0338330 MtrunA17_Chr6g0451381 57.143 105 37 2 150 250 202 302 6.71e-32 123
Msa0338330 MtrunA17_Chr8g0339101 55.046 109 41 2 146 250 235 339 2.06e-29 116
Msa0338330 MtrunA17_Chr3g0135191 51.429 105 43 2 150 250 236 336 1.12e-26 108
Msa0338330 MtrunA17_Chr4g0060331 73.016 63 13 2 13 72 28 89 1.42e-18 78.6
Msa0338330 MtrunA17_Chr4g0037831 74.194 62 12 2 14 72 29 89 3.71e-18 77.8
Msa0338330 MtrunA17_Chr1g0179441 40.000 105 55 2 150 250 86 186 1.93e-16 78.2
Msa0338330 MtrunA17_Chr1g0179421 41.121 107 51 3 150 250 86 186 2.40e-16 78.2
Msa0338330 MtrunA17_Chr1g0198111 38.211 123 64 3 136 250 75 193 5.44e-16 77.0
Msa0338330 MtrunA17_Chr7g0265711 41.905 105 53 2 150 250 95 195 6.64e-16 76.6
Msa0338330 MtrunA17_Chr7g0264621 40.000 105 55 2 150 250 91 191 8.00e-16 76.6
Msa0338330 MtrunA17_Chr1g0179381 40.187 107 52 3 150 250 86 186 9.41e-16 76.3
Msa0338330 MtrunA17_Chr4g0027221 37.143 105 58 2 150 250 95 195 4.27e-14 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0338330 AT2G24630.1 41.912 272 114 12 14 250 64 326 6.25e-39 143
Msa0338330 AT2G24630.2 41.912 272 114 12 14 250 64 326 6.25e-39 143
Msa0338330 AT4G31590.1 62.857 105 31 2 150 250 226 326 2.09e-35 133
Msa0338330 AT3G28180.1 35.424 271 120 10 14 250 49 298 5.68e-30 118
Msa0338330 AT3G07330.1 51.429 105 43 2 150 250 220 320 2.84e-28 113
Msa0338330 AT3G07330.2 51.429 105 43 2 150 250 220 320 2.84e-28 113
Msa0338330 AT4G07960.1 50.962 104 43 2 151 250 241 340 1.32e-25 105
Msa0338330 AT4G07960.2 50.962 104 43 2 151 250 241 340 1.32e-25 105
Msa0338330 AT5G03760.1 43.925 107 48 3 150 250 96 196 7.68e-17 79.7
Msa0338330 AT2G35650.4 42.056 107 50 3 150 250 46 146 4.43e-16 77.4
Msa0338330 AT2G35650.2 42.056 107 50 3 150 250 46 146 4.43e-16 77.4
Msa0338330 AT2G35650.1 42.056 107 50 3 150 250 118 218 4.54e-16 77.8
Msa0338330 AT4G13410.2 40.000 105 55 2 150 250 110 210 8.35e-16 76.6
Msa0338330 AT4G13410.1 40.000 105 55 2 150 250 110 210 1.38e-15 76.3
Msa0338330 AT1G24070.2 39.640 111 59 2 144 250 115 221 1.86e-15 75.5
Msa0338330 AT1G24070.1 40.952 105 54 2 150 250 121 221 2.44e-15 75.5
Msa0338330 AT1G23480.3 40.952 105 54 3 150 250 47 147 8.72e-15 73.9
Msa0338330 AT1G23480.1 40.952 105 54 3 150 250 119 219 9.47e-15 73.9
Msa0338330 AT1G23480.2 40.952 105 54 3 150 250 119 219 9.47e-15 73.9
Msa0338330 AT5G16190.1 38.095 105 57 2 150 250 12 112 4.94e-14 71.6
Msa0338330 AT5G16190.3 38.095 105 57 2 150 250 73 173 5.17e-14 71.6
Msa0338330 AT5G16190.2 38.095 105 57 2 150 250 73 173 6.09e-14 71.2
Msa0338330 AT5G16190.4 38.095 105 57 2 150 250 73 173 7.01e-14 71.2
Msa0338330 AT5G22740.1 36.190 105 59 2 150 250 96 196 9.34e-14 70.9
Msa0338330 AT3G56000.1 37.037 108 57 4 150 250 91 194 3.41e-11 63.2

Find 65 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGCATACAATGGTTAATT+AGG 0.180568 2_4:-58578826 Msa0338330:CDS
CACACTGAGTTTATCATATA+AGG 0.204376 2_4:-58578670 Msa0338330:exon
GGTGATTTCTTTGATTGTTT+TGG 0.224748 2_4:-58579771 Msa0338330:CDS
TATAATAAGTGATCAGATTA+TGG 0.225234 2_4:+58578641 None:intergenic
TATTGTGCTTACTTGGTTCT+AGG 0.228935 2_4:-58578610 Msa0338330:three_prime_UTR
GGCTCATCGCACCGTTTGTT+GGG 0.229582 2_4:-58579909 Msa0338330:CDS
GCACATTGCTCCACCCATTT+AGG 0.250648 2_4:-58579626 Msa0338330:CDS
GGGGTTGGTAACTGTTCTTC+TGG 0.257172 2_4:-58579888 Msa0338330:CDS
CCATTTAGGCTCTTTCAAAA+TGG 0.277923 2_4:-58579612 Msa0338330:CDS
GACCGTATGCTTCTTTGTTT+GGG 0.295829 2_4:-58579541 Msa0338330:CDS
AGTTTAAGCCAAGGATTAAC+AGG 0.300150 2_4:-58579495 Msa0338330:CDS
ATTCATTTATCCAATGGTTT+TGG 0.302694 2_4:-58579433 Msa0338330:CDS
TGGTTCTAGGATGTATGATT+TGG 0.317458 2_4:-58578597 Msa0338330:three_prime_UTR
GGACCGTATGCTTCTTTGTT+TGG 0.318486 2_4:-58579542 Msa0338330:CDS
GAAGCATACGGTCCACAAAT+TGG 0.320331 2_4:+58579551 None:intergenic
TTCTTTGTTTGGGGTGCTCT+TGG 0.337102 2_4:-58579531 Msa0338330:CDS
AGGCTCATCGCACCGTTTGT+TGG 0.338549 2_4:-58579910 Msa0338330:CDS
TTATAGCTTAATCGTTGTAT+TGG 0.347158 2_4:-58578548 Msa0338330:three_prime_UTR
TATAGCTTAATCGTTGTATT+GGG 0.355816 2_4:-58578547 Msa0338330:three_prime_UTR
CATAATCTATCGGCATTGTT+TGG 0.369705 2_4:-58578768 Msa0338330:CDS
TGGTAACTGTTCTTCTGGAT+TGG 0.382742 2_4:-58579883 Msa0338330:CDS
TTTCAAATATTGTGCTTACT+TGG 0.387549 2_4:-58578617 Msa0338330:three_prime_UTR
TTTGTTTCTTATCCAATTTG+TGG 0.388770 2_4:-58579563 Msa0338330:CDS
GGTTCTAGGATGTATGATTT+GGG 0.397116 2_4:-58578596 Msa0338330:three_prime_UTR
ATCTTCACAACTGAGAAATC+AGG 0.398750 2_4:+58580017 None:intergenic
AATGGATCCCTGTTAATCCT+TGG 0.419579 2_4:+58579487 None:intergenic
ACTGTTCTTCTGGATTGGCT+TGG 0.424815 2_4:-58579878 Msa0338330:CDS
TCAAAATGGCTTATGTTGCT+TGG 0.435336 2_4:-58579598 Msa0338330:CDS
CATTGTATGCTCTCATCATC+AGG 0.437248 2_4:+58578836 None:intergenic
GTCTCTAAGTGGAACCTAAC+GGG 0.443813 2_4:-58578797 Msa0338330:CDS
ACCAAGCAACAAAAGCCATT+TGG 0.447549 2_4:+58579660 None:intergenic
TTACTGATTCGAGTACTTGA+CGG 0.453690 2_4:-58578860 Msa0338330:CDS
GAGCATACAATGGTTAATTA+GGG 0.454001 2_4:-58578825 Msa0338330:CDS
CGGCATTGTTTGGTCAGCAC+TGG 0.456663 2_4:-58578758 Msa0338330:CDS
ATCTATATTCATTTATCCAA+TGG 0.460958 2_4:-58579439 Msa0338330:CDS
AATACATAGTCACAGCTCAT+TGG 0.463395 2_4:+58578710 None:intergenic
TCCAATGTGCAATGAGAAGC+AGG 0.467513 2_4:-58579403 Msa0338330:intron
TTTCTGAAGGTTGTTCCAAA+TGG 0.467998 2_4:-58579675 Msa0338330:intron
GGAATTTGAACCAAAACCAT+TGG 0.468674 2_4:+58579423 None:intergenic
TCAACATCATCAACCTTGAA+TGG 0.468739 2_4:+58579469 None:intergenic
AGATTATGTTGACTCCCGTT+AGG 0.480708 2_4:+58578783 None:intergenic
AAATTATCTCTGCATTTCAA+TGG 0.485431 2_4:+58578512 None:intergenic
GATTAACAGGGATCCATTCA+AGG 0.490147 2_4:-58579482 Msa0338330:CDS
GCTCATCGCACCGTTTGTTG+GGG 0.507752 2_4:-58579908 Msa0338330:CDS
TTCAAGGTTGATGATGTTGA+AGG 0.517442 2_4:-58579466 Msa0338330:CDS
TAGGATGTATGATTTGGGTA+AGG 0.525846 2_4:-58578591 Msa0338330:three_prime_UTR
AATTCAAGAAGTTTAAGCCA+AGG 0.560075 2_4:-58579504 Msa0338330:CDS
CTCATCGCACCGTTTGTTGG+GGG 0.560545 2_4:-58579907 Msa0338330:CDS
ACCTGCTTCTCATTGCACAT+TGG 0.565767 2_4:+58579402 None:intergenic
CGAATCAGTAAAAGATCACA+TGG 0.575054 2_4:+58578872 None:intergenic
CGGGAGTCAACATAATCTAT+CGG 0.577360 2_4:-58578778 Msa0338330:CDS
TCTTCACAACTGAGAAATCA+GGG 0.577459 2_4:+58580018 None:intergenic
GGTCTCTAAGTGGAACCTAA+CGG 0.582685 2_4:-58578798 Msa0338330:CDS
GATTAAGCTATAAGATCTCA+AGG 0.584279 2_4:+58578558 None:intergenic
GTATGCACAGGTGTATGCGC+AGG 0.584990 2_4:-58578924 Msa0338330:CDS
TAGGTTCCACTTAGAGACCT+CGG 0.606507 2_4:+58578802 None:intergenic
ACAATGGTTAATTAGGGCCG+AGG 0.607892 2_4:-58578819 Msa0338330:CDS
ATTGATTAGTAGACAAGGTG+TGG 0.611235 2_4:-58579840 Msa0338330:CDS
TTTGAAAGAGCCTAAATGGG+TGG 0.613559 2_4:+58579616 None:intergenic
AAAAGATTGATTAGTAGACA+AGG 0.621356 2_4:-58579845 Msa0338330:CDS
ACCGTATGCTTCTTTGTTTG+GGG 0.627675 2_4:-58579540 Msa0338330:CDS
ACCCCAAACAAAGAAGCATA+CGG 0.633312 2_4:+58579539 None:intergenic
TTAGGGCCGAGGTCTCTAAG+TGG 0.646091 2_4:-58578808 Msa0338330:CDS
GTTTAAGCCAAGGATTAACA+GGG 0.660650 2_4:-58579494 Msa0338330:CDS
CTGATGATGAGAGCATACAA+TGG 0.697198 2_4:-58578835 Msa0338330:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATAGATATCATTACTTT+AGG - chr2_4:58579358-58579377 Msa0338330:intron 15.0%
!!! CTTTTATCAAATTATTGAAA+TGG - chr2_4:58580026-58580045 Msa0338330:CDS 15.0%
!! ATTATAGATATCATTACTTT+AGG - chr2_4:58579358-58579377 Msa0338330:intron 15.0%
!!! CTTTTATCAAATTATTGAAA+TGG - chr2_4:58580026-58580045 Msa0338330:CDS 15.0%
!!! TAGAATCATTAGTTTGTTTT+TGG - chr2_4:58578696-58578715 Msa0338330:CDS 20.0%
!! ATCTATATTCATTTATCCAA+TGG - chr2_4:58579049-58579068 Msa0338330:intron 20.0%
!!! TTTTTAGATTTTAACTCCAT+TGG - chr2_4:58579254-58579273 Msa0338330:intron 20.0%
!!! TTTTAGATTTTAACTCCATT+GGG - chr2_4:58579255-58579274 Msa0338330:intron 20.0%
!! TATAATAAGTGATCAGATTA+TGG + chr2_4:58579850-58579869 None:intergenic 20.0%
!!! TAGAATCATTAGTTTGTTTT+TGG - chr2_4:58578696-58578715 Msa0338330:CDS 20.0%
!! ATCTATATTCATTTATCCAA+TGG - chr2_4:58579049-58579068 Msa0338330:intron 20.0%
!!! TTTTTAGATTTTAACTCCAT+TGG - chr2_4:58579254-58579273 Msa0338330:intron 20.0%
!!! TTTTAGATTTTAACTCCATT+GGG - chr2_4:58579255-58579274 Msa0338330:intron 20.0%
!! TATAATAAGTGATCAGATTA+TGG + chr2_4:58579850-58579869 None:intergenic 20.0%
! AAAAGATTGATTAGTAGACA+AGG - chr2_4:58578643-58578662 Msa0338330:three_prime_UTR 25.0%
!!! TTTGATTGTTTTGGATTTTG+AGG - chr2_4:58578726-58578745 Msa0338330:CDS 25.0%
!! TTTGTTTCTTATCCAATTTG+TGG - chr2_4:58578925-58578944 Msa0338330:CDS 25.0%
!! ATTCATTTATCCAATGGTTT+TGG - chr2_4:58579055-58579074 Msa0338330:intron 25.0%
!! TTTGTGTTACTAAGTATAAG+AGG + chr2_4:58579293-58579312 None:intergenic 25.0%
! TTAAACAACTCAACATCAAA+TGG + chr2_4:58579455-58579474 None:intergenic 25.0%
! TTATAAGCAATCAATGACAT+TGG - chr2_4:58579488-58579507 Msa0338330:CDS 25.0%
! TCAATGACATTGGTAAAATA+TGG - chr2_4:58579498-58579517 Msa0338330:CDS 25.0%
!! TTTGTTCATTTTGCACAAAT+TGG - chr2_4:58579530-58579549 Msa0338330:CDS 25.0%
! TTTCAAATATTGTGCTTACT+TGG - chr2_4:58579871-58579890 Msa0338330:CDS 25.0%
! TTATAGCTTAATCGTTGTAT+TGG - chr2_4:58579940-58579959 Msa0338330:intron 25.0%
! TATAGCTTAATCGTTGTATT+GGG - chr2_4:58579941-58579960 Msa0338330:intron 25.0%
! AAATTATCTCTGCATTTCAA+TGG + chr2_4:58579979-58579998 None:intergenic 25.0%
! AAAAGATTGATTAGTAGACA+AGG - chr2_4:58578643-58578662 Msa0338330:three_prime_UTR 25.0%
!!! TTTGATTGTTTTGGATTTTG+AGG - chr2_4:58578726-58578745 Msa0338330:CDS 25.0%
!! TTTGTTTCTTATCCAATTTG+TGG - chr2_4:58578925-58578944 Msa0338330:CDS 25.0%
!! ATTCATTTATCCAATGGTTT+TGG - chr2_4:58579055-58579074 Msa0338330:intron 25.0%
!! TTTGTGTTACTAAGTATAAG+AGG + chr2_4:58579293-58579312 None:intergenic 25.0%
! TTAAACAACTCAACATCAAA+TGG + chr2_4:58579455-58579474 None:intergenic 25.0%
! TTATAAGCAATCAATGACAT+TGG - chr2_4:58579488-58579507 Msa0338330:CDS 25.0%
! TCAATGACATTGGTAAAATA+TGG - chr2_4:58579498-58579517 Msa0338330:CDS 25.0%
!! TTTGTTCATTTTGCACAAAT+TGG - chr2_4:58579530-58579549 Msa0338330:CDS 25.0%
! TTTCAAATATTGTGCTTACT+TGG - chr2_4:58579871-58579890 Msa0338330:CDS 25.0%
! TTATAGCTTAATCGTTGTAT+TGG - chr2_4:58579940-58579959 Msa0338330:intron 25.0%
! TATAGCTTAATCGTTGTATT+GGG - chr2_4:58579941-58579960 Msa0338330:intron 25.0%
! AAATTATCTCTGCATTTCAA+TGG + chr2_4:58579979-58579998 None:intergenic 25.0%
TTCTCAGTTGTGAAGATTAA+TGG - chr2_4:58578477-58578496 Msa0338330:three_prime_UTR 30.0%
! GGTGATTTCTTTGATTGTTT+TGG - chr2_4:58578717-58578736 Msa0338330:CDS 30.0%
!!! CTCATACTTTTGATTTCTGA+AGG - chr2_4:58578800-58578819 Msa0338330:CDS 30.0%
!! CATTTTGAAAGAGCCTAAAT+GGG + chr2_4:58578878-58578897 None:intergenic 30.0%
AATTCAAGAAGTTTAAGCCA+AGG - chr2_4:58578984-58579003 Msa0338330:intron 30.0%
!!! TTGCACAAATTGGTTTTATC+AGG - chr2_4:58579540-58579559 Msa0338330:CDS 30.0%
AGAGCATACAATGGTTAATT+AGG - chr2_4:58579662-58579681 Msa0338330:CDS 30.0%
GAGCATACAATGGTTAATTA+GGG - chr2_4:58579663-58579682 Msa0338330:CDS 30.0%
CACACTGAGTTTATCATATA+AGG - chr2_4:58579818-58579837 Msa0338330:CDS 30.0%
GATTAAGCTATAAGATCTCA+AGG + chr2_4:58579933-58579952 None:intergenic 30.0%
TTCTCAGTTGTGAAGATTAA+TGG - chr2_4:58578477-58578496 Msa0338330:three_prime_UTR 30.0%
! GGTGATTTCTTTGATTGTTT+TGG - chr2_4:58578717-58578736 Msa0338330:CDS 30.0%
!!! CTCATACTTTTGATTTCTGA+AGG - chr2_4:58578800-58578819 Msa0338330:CDS 30.0%
!! CATTTTGAAAGAGCCTAAAT+GGG + chr2_4:58578878-58578897 None:intergenic 30.0%
AATTCAAGAAGTTTAAGCCA+AGG - chr2_4:58578984-58579003 Msa0338330:intron 30.0%
!!! TTGCACAAATTGGTTTTATC+AGG - chr2_4:58579540-58579559 Msa0338330:CDS 30.0%
AGAGCATACAATGGTTAATT+AGG - chr2_4:58579662-58579681 Msa0338330:CDS 30.0%
GAGCATACAATGGTTAATTA+GGG - chr2_4:58579663-58579682 Msa0338330:CDS 30.0%
CACACTGAGTTTATCATATA+AGG - chr2_4:58579818-58579837 Msa0338330:CDS 30.0%
GATTAAGCTATAAGATCTCA+AGG + chr2_4:58579933-58579952 None:intergenic 30.0%
TCTTCACAACTGAGAAATCA+GGG + chr2_4:58578473-58578492 None:intergenic 35.0%
ATCTTCACAACTGAGAAATC+AGG + chr2_4:58578474-58578493 None:intergenic 35.0%
ATTGATTAGTAGACAAGGTG+TGG - chr2_4:58578648-58578667 Msa0338330:three_prime_UTR 35.0%
! TTTCTGAAGGTTGTTCCAAA+TGG - chr2_4:58578813-58578832 Msa0338330:CDS 35.0%
!! CCATTTTGAAAGAGCCTAAA+TGG + chr2_4:58578879-58578898 None:intergenic 35.0%
CCATTTAGGCTCTTTCAAAA+TGG - chr2_4:58578876-58578895 Msa0338330:CDS 35.0%
TCAAAATGGCTTATGTTGCT+TGG - chr2_4:58578890-58578909 Msa0338330:CDS 35.0%
AGTTTAAGCCAAGGATTAAC+AGG - chr2_4:58578993-58579012 Msa0338330:intron 35.0%
GTTTAAGCCAAGGATTAACA+GGG - chr2_4:58578994-58579013 Msa0338330:intron 35.0%
TCAACATCATCAACCTTGAA+TGG + chr2_4:58579022-58579041 None:intergenic 35.0%
!! TTCAAGGTTGATGATGTTGA+AGG - chr2_4:58579022-58579041 Msa0338330:intron 35.0%
GGAATTTGAACCAAAACCAT+TGG + chr2_4:58579068-58579087 None:intergenic 35.0%
AAAAATGGAACGCAGAAGTT+TGG + chr2_4:58579118-58579137 None:intergenic 35.0%
ACATCACCTCTTAGCAAAAA+TGG + chr2_4:58579133-58579152 None:intergenic 35.0%
! GATTTTGCAAATGTCACAAG+AGG - chr2_4:58579212-58579231 Msa0338330:intron 35.0%
CGAATCAGTAAAAGATCACA+TGG + chr2_4:58579619-58579638 None:intergenic 35.0%
!! TTACTGATTCGAGTACTTGA+CGG - chr2_4:58579628-58579647 Msa0338330:CDS 35.0%
CATAATCTATCGGCATTGTT+TGG - chr2_4:58579720-58579739 Msa0338330:intron 35.0%
AATACATAGTCACAGCTCAT+TGG + chr2_4:58579781-58579800 None:intergenic 35.0%
! TATTGTGCTTACTTGGTTCT+AGG - chr2_4:58579878-58579897 Msa0338330:CDS 35.0%
!! TGGTTCTAGGATGTATGATT+TGG - chr2_4:58579891-58579910 Msa0338330:CDS 35.0%
!! GGTTCTAGGATGTATGATTT+GGG - chr2_4:58579892-58579911 Msa0338330:CDS 35.0%
! TAGGATGTATGATTTGGGTA+AGG - chr2_4:58579897-58579916 Msa0338330:CDS 35.0%
TCTTCACAACTGAGAAATCA+GGG + chr2_4:58578473-58578492 None:intergenic 35.0%
ATCTTCACAACTGAGAAATC+AGG + chr2_4:58578474-58578493 None:intergenic 35.0%
ATTGATTAGTAGACAAGGTG+TGG - chr2_4:58578648-58578667 Msa0338330:three_prime_UTR 35.0%
! TTTCTGAAGGTTGTTCCAAA+TGG - chr2_4:58578813-58578832 Msa0338330:CDS 35.0%
!! CCATTTTGAAAGAGCCTAAA+TGG + chr2_4:58578879-58578898 None:intergenic 35.0%
CCATTTAGGCTCTTTCAAAA+TGG - chr2_4:58578876-58578895 Msa0338330:CDS 35.0%
TCAAAATGGCTTATGTTGCT+TGG - chr2_4:58578890-58578909 Msa0338330:CDS 35.0%
AGTTTAAGCCAAGGATTAAC+AGG - chr2_4:58578993-58579012 Msa0338330:intron 35.0%
GTTTAAGCCAAGGATTAACA+GGG - chr2_4:58578994-58579013 Msa0338330:intron 35.0%
TCAACATCATCAACCTTGAA+TGG + chr2_4:58579022-58579041 None:intergenic 35.0%
!! TTCAAGGTTGATGATGTTGA+AGG - chr2_4:58579022-58579041 Msa0338330:intron 35.0%
GGAATTTGAACCAAAACCAT+TGG + chr2_4:58579068-58579087 None:intergenic 35.0%
AAAAATGGAACGCAGAAGTT+TGG + chr2_4:58579118-58579137 None:intergenic 35.0%
ACATCACCTCTTAGCAAAAA+TGG + chr2_4:58579133-58579152 None:intergenic 35.0%
! GATTTTGCAAATGTCACAAG+AGG - chr2_4:58579212-58579231 Msa0338330:intron 35.0%
CGAATCAGTAAAAGATCACA+TGG + chr2_4:58579619-58579638 None:intergenic 35.0%
!! TTACTGATTCGAGTACTTGA+CGG - chr2_4:58579628-58579647 Msa0338330:CDS 35.0%
CATAATCTATCGGCATTGTT+TGG - chr2_4:58579720-58579739 Msa0338330:intron 35.0%
AATACATAGTCACAGCTCAT+TGG + chr2_4:58579781-58579800 None:intergenic 35.0%
! TATTGTGCTTACTTGGTTCT+AGG - chr2_4:58579878-58579897 Msa0338330:CDS 35.0%
!! TGGTTCTAGGATGTATGATT+TGG - chr2_4:58579891-58579910 Msa0338330:CDS 35.0%
!! GGTTCTAGGATGTATGATTT+GGG - chr2_4:58579892-58579911 Msa0338330:CDS 35.0%
! TAGGATGTATGATTTGGGTA+AGG - chr2_4:58579897-58579916 Msa0338330:CDS 35.0%
! GATGCTGACATTTAGGTTTG+TGG - chr2_4:58578504-58578523 Msa0338330:three_prime_UTR 40.0%
AGGTTTGTGGATAAAAACCG+CGG - chr2_4:58578517-58578536 Msa0338330:three_prime_UTR 40.0%
! TACGTGAGTTTTGCTTCTCA+AGG - chr2_4:58578558-58578577 Msa0338330:three_prime_UTR 40.0%
TGGTAACTGTTCTTCTGGAT+TGG - chr2_4:58578605-58578624 Msa0338330:three_prime_UTR 40.0%
! TTGTTGCTTACACTCAAGGT+TGG - chr2_4:58578749-58578768 Msa0338330:CDS 40.0%
! ACACTCAAGGTTGGCATTTT+AGG - chr2_4:58578758-58578777 Msa0338330:CDS 40.0%
! TCCAAATGGCTTTTGTTGCT+TGG - chr2_4:58578827-58578846 Msa0338330:CDS 40.0%
ACCAAGCAACAAAAGCCATT+TGG + chr2_4:58578831-58578850 None:intergenic 40.0%
TTTGAAAGAGCCTAAATGGG+TGG + chr2_4:58578875-58578894 None:intergenic 40.0%
! GACCGTATGCTTCTTTGTTT+GGG - chr2_4:58578947-58578966 Msa0338330:intron 40.0%
! ACCGTATGCTTCTTTGTTTG+GGG - chr2_4:58578948-58578967 Msa0338330:intron 40.0%
! ACCCCAAACAAAGAAGCATA+CGG + chr2_4:58578952-58578971 None:intergenic 40.0%
AATGGATCCCTGTTAATCCT+TGG + chr2_4:58579004-58579023 None:intergenic 40.0%
GATTAACAGGGATCCATTCA+AGG - chr2_4:58579006-58579025 Msa0338330:intron 40.0%
!! TGCGTTCCATTTTTGCTAAG+AGG - chr2_4:58579124-58579143 Msa0338330:intron 40.0%
ACAATTGCACTGAAACTTGC+TGG + chr2_4:58579164-58579183 None:intergenic 40.0%
AGGTTGCAGATAATTCCCAA+TGG + chr2_4:58579273-58579292 None:intergenic 40.0%
! GTTTTATCAGGTGTATGCAC+AGG - chr2_4:58579552-58579571 Msa0338330:CDS 40.0%
CATTGTATGCTCTCATCATC+AGG + chr2_4:58579655-58579674 None:intergenic 40.0%
! CTGATGATGAGAGCATACAA+TGG - chr2_4:58579653-58579672 Msa0338330:CDS 40.0%
!! AGATTATGTTGACTCCCGTT+AGG + chr2_4:58579708-58579727 None:intergenic 40.0%
CGGGAGTCAACATAATCTAT+CGG - chr2_4:58579710-58579729 Msa0338330:intron 40.0%
! GATGCTGACATTTAGGTTTG+TGG - chr2_4:58578504-58578523 Msa0338330:three_prime_UTR 40.0%
AGGTTTGTGGATAAAAACCG+CGG - chr2_4:58578517-58578536 Msa0338330:three_prime_UTR 40.0%
! TACGTGAGTTTTGCTTCTCA+AGG - chr2_4:58578558-58578577 Msa0338330:three_prime_UTR 40.0%
TGGTAACTGTTCTTCTGGAT+TGG - chr2_4:58578605-58578624 Msa0338330:three_prime_UTR 40.0%
! TTGTTGCTTACACTCAAGGT+TGG - chr2_4:58578749-58578768 Msa0338330:CDS 40.0%
! ACACTCAAGGTTGGCATTTT+AGG - chr2_4:58578758-58578777 Msa0338330:CDS 40.0%
! TCCAAATGGCTTTTGTTGCT+TGG - chr2_4:58578827-58578846 Msa0338330:CDS 40.0%
ACCAAGCAACAAAAGCCATT+TGG + chr2_4:58578831-58578850 None:intergenic 40.0%
TTTGAAAGAGCCTAAATGGG+TGG + chr2_4:58578875-58578894 None:intergenic 40.0%
! GACCGTATGCTTCTTTGTTT+GGG - chr2_4:58578947-58578966 Msa0338330:intron 40.0%
! ACCGTATGCTTCTTTGTTTG+GGG - chr2_4:58578948-58578967 Msa0338330:intron 40.0%
! ACCCCAAACAAAGAAGCATA+CGG + chr2_4:58578952-58578971 None:intergenic 40.0%
AATGGATCCCTGTTAATCCT+TGG + chr2_4:58579004-58579023 None:intergenic 40.0%
GATTAACAGGGATCCATTCA+AGG - chr2_4:58579006-58579025 Msa0338330:intron 40.0%
!! TGCGTTCCATTTTTGCTAAG+AGG - chr2_4:58579124-58579143 Msa0338330:intron 40.0%
ACAATTGCACTGAAACTTGC+TGG + chr2_4:58579164-58579183 None:intergenic 40.0%
AGGTTGCAGATAATTCCCAA+TGG + chr2_4:58579273-58579292 None:intergenic 40.0%
! GTTTTATCAGGTGTATGCAC+AGG - chr2_4:58579552-58579571 Msa0338330:CDS 40.0%
CATTGTATGCTCTCATCATC+AGG + chr2_4:58579655-58579674 None:intergenic 40.0%
! CTGATGATGAGAGCATACAA+TGG - chr2_4:58579653-58579672 Msa0338330:CDS 40.0%
!! AGATTATGTTGACTCCCGTT+AGG + chr2_4:58579708-58579727 None:intergenic 40.0%
CGGGAGTCAACATAATCTAT+CGG - chr2_4:58579710-58579729 Msa0338330:intron 40.0%
TGGTGCAGATGCTGACATTT+AGG - chr2_4:58578497-58578516 Msa0338330:three_prime_UTR 45.0%
CTTGTATAACATTCCTGCCG+CGG + chr2_4:58578537-58578556 None:intergenic 45.0%
AGTTACCAACCCCCAACAAA+CGG + chr2_4:58578593-58578612 None:intergenic 45.0%
!! ACTGTTCTTCTGGATTGGCT+TGG - chr2_4:58578610-58578629 Msa0338330:three_prime_UTR 45.0%
! GAGGTTGTTGCTTACACTCA+AGG - chr2_4:58578745-58578764 Msa0338330:CDS 45.0%
GAAGCATACGGTCCACAAAT+TGG + chr2_4:58578940-58578959 None:intergenic 45.0%
GGACCGTATGCTTCTTTGTT+TGG - chr2_4:58578946-58578965 Msa0338330:intron 45.0%
! TTCTTTGTTTGGGGTGCTCT+TGG - chr2_4:58578957-58578976 Msa0338330:intron 45.0%
TCCAATGTGCAATGAGAAGC+AGG - chr2_4:58579085-58579104 Msa0338330:intron 45.0%
ACCTGCTTCTCATTGCACAT+TGG + chr2_4:58579089-58579108 None:intergenic 45.0%
ACAATGGTTAATTAGGGCCG+AGG - chr2_4:58579669-58579688 Msa0338330:CDS 45.0%
TAGGTTCCACTTAGAGACCT+CGG + chr2_4:58579689-58579708 None:intergenic 45.0%
GGTCTCTAAGTGGAACCTAA+CGG - chr2_4:58579690-58579709 Msa0338330:intron 45.0%
GTCTCTAAGTGGAACCTAAC+GGG - chr2_4:58579691-58579710 Msa0338330:intron 45.0%
TGGTGCAGATGCTGACATTT+AGG - chr2_4:58578497-58578516 Msa0338330:three_prime_UTR 45.0%
CTTGTATAACATTCCTGCCG+CGG + chr2_4:58578537-58578556 None:intergenic 45.0%
AGTTACCAACCCCCAACAAA+CGG + chr2_4:58578593-58578612 None:intergenic 45.0%
!! ACTGTTCTTCTGGATTGGCT+TGG - chr2_4:58578610-58578629 Msa0338330:three_prime_UTR 45.0%
! GAGGTTGTTGCTTACACTCA+AGG - chr2_4:58578745-58578764 Msa0338330:CDS 45.0%
GAAGCATACGGTCCACAAAT+TGG + chr2_4:58578940-58578959 None:intergenic 45.0%
GGACCGTATGCTTCTTTGTT+TGG - chr2_4:58578946-58578965 Msa0338330:intron 45.0%
! TTCTTTGTTTGGGGTGCTCT+TGG - chr2_4:58578957-58578976 Msa0338330:intron 45.0%
TCCAATGTGCAATGAGAAGC+AGG - chr2_4:58579085-58579104 Msa0338330:intron 45.0%
ACCTGCTTCTCATTGCACAT+TGG + chr2_4:58579089-58579108 None:intergenic 45.0%
ACAATGGTTAATTAGGGCCG+AGG - chr2_4:58579669-58579688 Msa0338330:CDS 45.0%
TAGGTTCCACTTAGAGACCT+CGG + chr2_4:58579689-58579708 None:intergenic 45.0%
GGTCTCTAAGTGGAACCTAA+CGG - chr2_4:58579690-58579709 Msa0338330:intron 45.0%
GTCTCTAAGTGGAACCTAAC+GGG - chr2_4:58579691-58579710 Msa0338330:intron 45.0%
TTGTGGATAAAAACCGCGGC+AGG - chr2_4:58578521-58578540 Msa0338330:three_prime_UTR 50.0%
!! GGGGTTGGTAACTGTTCTTC+TGG - chr2_4:58578600-58578619 Msa0338330:three_prime_UTR 50.0%
GCACATTGCTCCACCCATTT+AGG - chr2_4:58578862-58578881 Msa0338330:CDS 50.0%
TTGTGGATAAAAACCGCGGC+AGG - chr2_4:58578521-58578540 Msa0338330:three_prime_UTR 50.0%
!! GGGGTTGGTAACTGTTCTTC+TGG - chr2_4:58578600-58578619 Msa0338330:three_prime_UTR 50.0%
GCACATTGCTCCACCCATTT+AGG - chr2_4:58578862-58578881 Msa0338330:CDS 50.0%
AGGCTCATCGCACCGTTTGT+TGG - chr2_4:58578578-58578597 Msa0338330:three_prime_UTR 55.0%
GGCTCATCGCACCGTTTGTT+GGG - chr2_4:58578579-58578598 Msa0338330:three_prime_UTR 55.0%
! GCTCATCGCACCGTTTGTTG+GGG - chr2_4:58578580-58578599 Msa0338330:three_prime_UTR 55.0%
! CTCATCGCACCGTTTGTTGG+GGG - chr2_4:58578581-58578600 Msa0338330:three_prime_UTR 55.0%
GTATGCACAGGTGTATGCGC+AGG - chr2_4:58579564-58579583 Msa0338330:CDS 55.0%
TTAGGGCCGAGGTCTCTAAG+TGG - chr2_4:58579680-58579699 Msa0338330:intron 55.0%
CGGCATTGTTTGGTCAGCAC+TGG - chr2_4:58579730-58579749 Msa0338330:intron 55.0%
AGGCTCATCGCACCGTTTGT+TGG - chr2_4:58578578-58578597 Msa0338330:three_prime_UTR 55.0%
GGCTCATCGCACCGTTTGTT+GGG - chr2_4:58578579-58578598 Msa0338330:three_prime_UTR 55.0%
! GCTCATCGCACCGTTTGTTG+GGG - chr2_4:58578580-58578599 Msa0338330:three_prime_UTR 55.0%
! CTCATCGCACCGTTTGTTGG+GGG - chr2_4:58578581-58578600 Msa0338330:three_prime_UTR 55.0%
GTATGCACAGGTGTATGCGC+AGG - chr2_4:58579564-58579583 Msa0338330:CDS 55.0%
TTAGGGCCGAGGTCTCTAAG+TGG - chr2_4:58579680-58579699 Msa0338330:intron 55.0%
CGGCATTGTTTGGTCAGCAC+TGG - chr2_4:58579730-58579749 Msa0338330:intron 55.0%
! TCGCACCGTTTGTTGGGGGT+TGG - chr2_4:58578585-58578604 Msa0338330:three_prime_UTR 60.0%
! TCGCACCGTTTGTTGGGGGT+TGG - chr2_4:58578585-58578604 Msa0338330:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 58578447 58580063 58578447 ID=Msa0338330;Name=Msa0338330
chr2_4 mRNA 58578447 58580063 58578447 ID=Msa0338330-mRNA-1;Parent=Msa0338330;Name=Msa0338330-mRNA-1;_AED=0.29;_eAED=0.32;_QI=0|0.33|0.5|1|1|1|4|238|251
chr2_4 exon 58578447 58578948 58578447 ID=Msa0338330-mRNA-1:exon:18081;Parent=Msa0338330-mRNA-1
chr2_4 exon 58579404 58579688 58579404 ID=Msa0338330-mRNA-1:exon:18080;Parent=Msa0338330-mRNA-1
chr2_4 exon 58579763 58579930 58579763 ID=Msa0338330-mRNA-1:exon:18079;Parent=Msa0338330-mRNA-1
chr2_4 exon 58580025 58580063 58580025 ID=Msa0338330-mRNA-1:exon:18078;Parent=Msa0338330-mRNA-1
chr2_4 CDS 58580025 58580063 58580025 ID=Msa0338330-mRNA-1:cds;Parent=Msa0338330-mRNA-1
chr2_4 CDS 58579763 58579930 58579763 ID=Msa0338330-mRNA-1:cds;Parent=Msa0338330-mRNA-1
chr2_4 CDS 58579404 58579688 58579404 ID=Msa0338330-mRNA-1:cds;Parent=Msa0338330-mRNA-1
chr2_4 CDS 58578685 58578948 58578685 ID=Msa0338330-mRNA-1:cds;Parent=Msa0338330-mRNA-1
chr2_4 three_prime_UTR 58578447 58578684 58578447 ID=Msa0338330-mRNA-1:three_prime_utr;Parent=Msa0338330-mRNA-1
Gene Sequence

>Msa0338330

ATGGGGGGTGTGAAAATGGAGGACCCTGATTTCTCAGTTGCTCATCGCACCGTTTGTTGGGGGTTGGTAACTGTTCTTCTGGATTGGCTTGGAGCTATTAAGAAAAGATTGATTAGTAGACAAGGTGTGGCTGTTGAAAAATGTAAATTGTTGTTTAGAATCATTAGTTTGTTTTTGGTGATTTCTTTGATTGTTTTGGATTTTGAGGTTGTTCCAAATGGCTTTTGTTGCTTGGTTGATGTTTCATGCACATTGCTCCACCCATTTAGGCTCTTTCAAAATGGCTTATGTTGCTTGGATTTTGTATTGTTTTGTTTCTTATCCAATTTGTGGACCGTATGCTTCTTTGTTTGGGGTGCTCTTGGATCAAATTCAAGAAGTTTAAGCCAAGGATTAACAGGGATCCATTCAAGGTTGATGATGTTGAAGGATCTATCTATATTCATTTATCCAATGGTTTTGGTTCAAATTCCAATGTGCAATGAGAAGCAGGTGTATGCACAGGTGTATGCGCAGGCAATTTCTGCAATCTGTCAAATTGATTATCCATGTGATCTTTTACTGATTCGAGTACTTGACGGTCCTGATGATGAGAGCATACAATGGTTAATTAGGGCCGAGGTCTCTAAGTGGAACCTAACGGGAGTCAACATAATCTATCGGCATTGTTTGGTCAGCACTGGATATGAAGTTAGAAAACTGAATTCTCCAATGAGCTGTGACTATGTATTTTTTGCAATATTTGATGCACACTGA
Protein sequence

>Msa0338330

MGGVKMEDPDFSVAHRTVCWGLVTVLLDWLGAIKKRLISRQGVAVEKCKLLFRIISLFLVISLIVLDFEVVPNGFCCLVDVSCTLLHPFRLFQNGLCCLDFVLFCFLSNLWTVCFFVWGALGSNSRSLSQGLTGIHSRLMMLKDLSIFIYPMVLVQIPMCNEKQVYAQVYAQAISAICQIDYPCDLLLIRVLDGPDDESIQWLIRAEVSKWNLTGVNIIYRHCLVSTGYEVRKLNSPMSCDYVFFAIFDAH*