Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0221060 | Msa0340180 | 0.801528 | 8.631293e-49 | -8.615850e-47 |
| Msa0262510 | Msa0340180 | 0.805759 | 1.143197e-49 | -8.615850e-47 |
| Msa0297960 | Msa0340180 | 0.802291 | 6.016154e-49 | -8.615850e-47 |
| Msa0338110 | Msa0340180 | 0.800397 | 1.469355e-48 | -8.615850e-47 |
| Msa0340180 | Msa0376590 | 0.800545 | 1.370347e-48 | -8.615850e-47 |
| Msa0340180 | Msa0388960 | 0.822337 | 2.500592e-53 | -8.615850e-47 |
| Msa0340180 | Msa0399300 | 0.801217 | 9.992357e-49 | -8.615850e-47 |
| Msa0340180 | Msa0484780 | 0.804437 | 2.161150e-49 | -8.615850e-47 |
| Msa0340180 | Msa0658570 | 0.803849 | 2.864793e-49 | -8.615850e-47 |
| Msa0340180 | Msa0693380 | 0.805690 | 1.181950e-49 | -8.615850e-47 |
| Msa0340180 | Msa0713640 | 0.806376 | 8.475681e-50 | -8.615850e-47 |
| Msa0340180 | Msa0870530 | 0.802633 | 5.114501e-49 | -8.615850e-47 |
| Msa0340180 | Msa1080160 | 0.805029 | 1.625811e-49 | -8.615850e-47 |
| Msa0340180 | Msa1082500 | 0.814442 | 1.537514e-51 | -8.615850e-47 |
| Msa0340180 | Msa1313150 | 0.821381 | 4.161300e-53 | -8.615850e-47 |
| Msa0340180 | Msa1423880 | 0.832138 | 1.121680e-55 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 76 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATTACTTGTGTCGTATA+GGG | 0.294288 | 2_4:-61488329 | Msa0340180:CDS |
| TGAAATGAAAGGATTCTATA+AGG | 0.340207 | 2_4:-61488374 | Msa0340180:CDS |
| TTCATATATGCCAACATTGC+TGG | 0.340216 | 2_4:+61488277 | None:intergenic |
| TAAGGATTGGAAAGCAGATT+TGG | 0.345156 | 2_4:-61488356 | Msa0340180:CDS |
| TACATTGAGGTCAATGATAA+AGG | 0.359321 | 2_4:-61488401 | Msa0340180:CDS |
| TCAGAGAGGTCAGTATAATC+AGG | 0.455856 | 2_4:+61488241 | None:intergenic |
| TGAAAGGATTCTATAAGGAT+TGG | 0.457239 | 2_4:-61488369 | Msa0340180:CDS |
| TTTATCATTGACCTCAATGT+AGG | 0.475914 | 2_4:+61488403 | None:intergenic |
| ACAACAACAAGATCAGGGTT+TGG | 0.506743 | 2_4:+61487585 | None:intergenic |
| GAATTTCGCGAGATCATTGT+GGG | 0.512647 | 2_4:-61488307 | Msa0340180:CDS |
| ATAAAGGATCTTGAAATGAA+AGG | 0.544451 | 2_4:-61488385 | Msa0340180:CDS |
| TAGTTTGCAAGATTTAAAGC+TGG | 0.573475 | 2_4:-61487909 | Msa0340180:CDS |
| GGAATTTCGCGAGATCATTG+TGG | 0.593486 | 2_4:-61488308 | Msa0340180:CDS |
| AAGAGACAATGCCTACATTG+AGG | 0.621357 | 2_4:-61488414 | None:intergenic |
| TTATACTGACCTCTCTGATG+TGG | 0.626845 | 2_4:-61488236 | Msa0340180:CDS |
| GACCTCTCTGATGTGGACTG+TGG | 0.632808 | 2_4:-61488229 | Msa0340180:CDS |
| GGGTCTGAGACCAGCAATGT+TGG | 0.635021 | 2_4:-61488287 | Msa0340180:CDS |
| CTCCACAGTCCACATCAGAG+AGG | 0.735772 | 2_4:+61488227 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATAAAAGATATATAGTATT+GGG | + | chr2_4:61488178-61488197 | None:intergenic | 10.0% |
| !! | ATATAAAAGATATATAGTAT+TGG | + | chr2_4:61488179-61488198 | None:intergenic | 10.0% |
| !! | TATAAAAGATATATAGTATT+GGG | + | chr2_4:61488178-61488197 | None:intergenic | 10.0% |
| !! | ATATAAAAGATATATAGTAT+TGG | + | chr2_4:61488179-61488198 | None:intergenic | 10.0% |
| !! | TAAAGATAAGCATATATCAT+AGG | + | chr2_4:61487793-61487812 | None:intergenic | 20.0% |
| !! | GTGAAAACAAAAATATAAGA+TGG | + | chr2_4:61487971-61487990 | None:intergenic | 20.0% |
| !!! | AAATAGCCATCATTATTTTA+TGG | + | chr2_4:61487999-61488018 | None:intergenic | 20.0% |
| !! | TAAAGATAAGCATATATCAT+AGG | + | chr2_4:61487793-61487812 | None:intergenic | 20.0% |
| !! | GTGAAAACAAAAATATAAGA+TGG | + | chr2_4:61487971-61487990 | None:intergenic | 20.0% |
| !!! | AAATAGCCATCATTATTTTA+TGG | + | chr2_4:61487999-61488018 | None:intergenic | 20.0% |
| ! | ATAAAGGATCTTGAAATGAA+AGG | - | chr2_4:61487578-61487597 | Msa0340180:CDS | 25.0% |
| ! | TGAAATGAAAGGATTCTATA+AGG | - | chr2_4:61487589-61487608 | Msa0340180:intron | 25.0% |
| ! | AAAAATTACTTGTGTCGTAT+AGG | - | chr2_4:61487633-61487652 | Msa0340180:intron | 25.0% |
| ! | AAAATTACTTGTGTCGTATA+GGG | - | chr2_4:61487634-61487653 | Msa0340180:intron | 25.0% |
| ! | AAAATTTAGCACCAGAAAAT+GGG | + | chr2_4:61487871-61487890 | None:intergenic | 25.0% |
| ! | TAAAATTTAGCACCAGAAAA+TGG | + | chr2_4:61487872-61487891 | None:intergenic | 25.0% |
| !!! | CCATCATTATTTTATGGAAA+AGG | + | chr2_4:61487993-61488012 | None:intergenic | 25.0% |
| !! | CCTTTTCCATAAAATAATGA+TGG | - | chr2_4:61487990-61488009 | Msa0340180:intron | 25.0% |
| ! | ATAAAGGATCTTGAAATGAA+AGG | - | chr2_4:61487578-61487597 | Msa0340180:CDS | 25.0% |
| ! | TGAAATGAAAGGATTCTATA+AGG | - | chr2_4:61487589-61487608 | Msa0340180:intron | 25.0% |
| ! | AAAAATTACTTGTGTCGTAT+AGG | - | chr2_4:61487633-61487652 | Msa0340180:intron | 25.0% |
| ! | AAAATTACTTGTGTCGTATA+GGG | - | chr2_4:61487634-61487653 | Msa0340180:intron | 25.0% |
| ! | AAAATTTAGCACCAGAAAAT+GGG | + | chr2_4:61487871-61487890 | None:intergenic | 25.0% |
| ! | TAAAATTTAGCACCAGAAAA+TGG | + | chr2_4:61487872-61487891 | None:intergenic | 25.0% |
| !!! | CCATCATTATTTTATGGAAA+AGG | + | chr2_4:61487993-61488012 | None:intergenic | 25.0% |
| !! | CCTTTTCCATAAAATAATGA+TGG | - | chr2_4:61487990-61488009 | Msa0340180:intron | 25.0% |
| TTTATCATTGACCTCAATGT+AGG | + | chr2_4:61487563-61487582 | None:intergenic | 30.0% | |
| TACATTGAGGTCAATGATAA+AGG | - | chr2_4:61487562-61487581 | Msa0340180:CDS | 30.0% | |
| TGAAAGGATTCTATAAGGAT+TGG | - | chr2_4:61487594-61487613 | Msa0340180:intron | 30.0% | |
| !! | TGATCCTAATATCGATTTAC+AGG | - | chr2_4:61487827-61487846 | Msa0340180:intron | 30.0% |
| ! | AATTCACATTTCCCATTTTC+TGG | - | chr2_4:61487857-61487876 | Msa0340180:intron | 30.0% |
| TTTCATGGCTTCTAATTATG+AGG | + | chr2_4:61487911-61487930 | None:intergenic | 30.0% | |
| ! | ACCTATATGCTATGTTTTCA+TGG | + | chr2_4:61487926-61487945 | None:intergenic | 30.0% |
| ! | TAGTTTGCAAGATTTAAAGC+TGG | - | chr2_4:61488054-61488073 | Msa0340180:intron | 30.0% |
| TTCAATCAACAATGAACATG+TGG | + | chr2_4:61488357-61488376 | None:intergenic | 30.0% | |
| TTTATCATTGACCTCAATGT+AGG | + | chr2_4:61487563-61487582 | None:intergenic | 30.0% | |
| TACATTGAGGTCAATGATAA+AGG | - | chr2_4:61487562-61487581 | Msa0340180:CDS | 30.0% | |
| TGAAAGGATTCTATAAGGAT+TGG | - | chr2_4:61487594-61487613 | Msa0340180:intron | 30.0% | |
| !! | TGATCCTAATATCGATTTAC+AGG | - | chr2_4:61487827-61487846 | Msa0340180:intron | 30.0% |
| ! | AATTCACATTTCCCATTTTC+TGG | - | chr2_4:61487857-61487876 | Msa0340180:intron | 30.0% |
| TTTCATGGCTTCTAATTATG+AGG | + | chr2_4:61487911-61487930 | None:intergenic | 30.0% | |
| ! | ACCTATATGCTATGTTTTCA+TGG | + | chr2_4:61487926-61487945 | None:intergenic | 30.0% |
| ! | TAGTTTGCAAGATTTAAAGC+TGG | - | chr2_4:61488054-61488073 | Msa0340180:intron | 30.0% |
| TTCAATCAACAATGAACATG+TGG | + | chr2_4:61488357-61488376 | None:intergenic | 30.0% | |
| TAAGGATTGGAAAGCAGATT+TGG | - | chr2_4:61487607-61487626 | Msa0340180:intron | 35.0% | |
| TTCATATATGCCAACATTGC+TGG | + | chr2_4:61487689-61487708 | None:intergenic | 35.0% | |
| CCAACCTGTAAATCGATATT+AGG | + | chr2_4:61487834-61487853 | None:intergenic | 35.0% | |
| !! | CCTAATATCGATTTACAGGT+TGG | - | chr2_4:61487831-61487850 | Msa0340180:intron | 35.0% |
| GCCATGAAAACATAGCATAT+AGG | - | chr2_4:61487922-61487941 | Msa0340180:CDS | 35.0% | |
| CAACAACAACAACAAGATCA+GGG | + | chr2_4:61488386-61488405 | None:intergenic | 35.0% | |
| ACAACAACAACAACAAGATC+AGG | + | chr2_4:61488387-61488406 | None:intergenic | 35.0% | |
| TAAGGATTGGAAAGCAGATT+TGG | - | chr2_4:61487607-61487626 | Msa0340180:intron | 35.0% | |
| TTCATATATGCCAACATTGC+TGG | + | chr2_4:61487689-61487708 | None:intergenic | 35.0% | |
| CCAACCTGTAAATCGATATT+AGG | + | chr2_4:61487834-61487853 | None:intergenic | 35.0% | |
| !! | CCTAATATCGATTTACAGGT+TGG | - | chr2_4:61487831-61487850 | Msa0340180:intron | 35.0% |
| GCCATGAAAACATAGCATAT+AGG | - | chr2_4:61487922-61487941 | Msa0340180:CDS | 35.0% | |
| CAACAACAACAACAAGATCA+GGG | + | chr2_4:61488386-61488405 | None:intergenic | 35.0% | |
| ACAACAACAACAACAAGATC+AGG | + | chr2_4:61488387-61488406 | None:intergenic | 35.0% | |
| GAATTTCGCGAGATCATTGT+GGG | - | chr2_4:61487656-61487675 | Msa0340180:intron | 40.0% | |
| TCAGAGAGGTCAGTATAATC+AGG | + | chr2_4:61487725-61487744 | None:intergenic | 40.0% | |
| TTATACTGACCTCTCTGATG+TGG | - | chr2_4:61487727-61487746 | Msa0340180:intron | 40.0% | |
| ! | TCTTGATAACAAGTCGTGCA+AGG | - | chr2_4:61488148-61488167 | Msa0340180:intron | 40.0% |
| ACAACAACAAGATCAGGGTT+TGG | + | chr2_4:61488381-61488400 | None:intergenic | 40.0% | |
| GAATTTCGCGAGATCATTGT+GGG | - | chr2_4:61487656-61487675 | Msa0340180:intron | 40.0% | |
| TCAGAGAGGTCAGTATAATC+AGG | + | chr2_4:61487725-61487744 | None:intergenic | 40.0% | |
| TTATACTGACCTCTCTGATG+TGG | - | chr2_4:61487727-61487746 | Msa0340180:intron | 40.0% | |
| ! | TCTTGATAACAAGTCGTGCA+AGG | - | chr2_4:61488148-61488167 | Msa0340180:intron | 40.0% |
| ACAACAACAAGATCAGGGTT+TGG | + | chr2_4:61488381-61488400 | None:intergenic | 40.0% | |
| GGAATTTCGCGAGATCATTG+TGG | - | chr2_4:61487655-61487674 | Msa0340180:intron | 45.0% | |
| GGAATTTCGCGAGATCATTG+TGG | - | chr2_4:61487655-61487674 | Msa0340180:intron | 45.0% | |
| ! | GGGTCTGAGACCAGCAATGT+TGG | - | chr2_4:61487676-61487695 | Msa0340180:intron | 55.0% |
| ! | GACCTCTCTGATGTGGACTG+TGG | - | chr2_4:61487734-61487753 | Msa0340180:intron | 55.0% |
| CTCCACAGTCCACATCAGAG+AGG | + | chr2_4:61487739-61487758 | None:intergenic | 55.0% | |
| ! | GGGTCTGAGACCAGCAATGT+TGG | - | chr2_4:61487676-61487695 | Msa0340180:intron | 55.0% |
| ! | GACCTCTCTGATGTGGACTG+TGG | - | chr2_4:61487734-61487753 | Msa0340180:intron | 55.0% |
| CTCCACAGTCCACATCAGAG+AGG | + | chr2_4:61487739-61487758 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_4 | gene | 61487557 | 61488428 | 61487557 | ID=Msa0340180;Name=Msa0340180 |
| chr2_4 | mRNA | 61487557 | 61488428 | 61487557 | ID=Msa0340180-mRNA-1;Parent=Msa0340180;Name=Msa0340180-mRNA-1;_AED=0.66;_eAED=0.71;_QI=0|0|0|0.66|1|1|3|0|119 |
| chr2_4 | exon | 61488199 | 61488428 | 61488199 | ID=Msa0340180-mRNA-1:exon:19411;Parent=Msa0340180-mRNA-1 |
| chr2_4 | exon | 61487868 | 61487946 | 61487868 | ID=Msa0340180-mRNA-1:exon:19410;Parent=Msa0340180-mRNA-1 |
| chr2_4 | exon | 61487557 | 61487607 | 61487557 | ID=Msa0340180-mRNA-1:exon:19409;Parent=Msa0340180-mRNA-1 |
| chr2_4 | CDS | 61488199 | 61488428 | 61488199 | ID=Msa0340180-mRNA-1:cds;Parent=Msa0340180-mRNA-1 |
| chr2_4 | CDS | 61487868 | 61487946 | 61487868 | ID=Msa0340180-mRNA-1:cds;Parent=Msa0340180-mRNA-1 |
| chr2_4 | CDS | 61487557 | 61487607 | 61487557 | ID=Msa0340180-mRNA-1:cds;Parent=Msa0340180-mRNA-1 |
| Gene Sequence |
| Protein sequence |
