AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0352220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0352220 MtrunA17_Chr2g0284681 92.231 605 42 2 1 602 1 603 0.0 1142
Msa0352220 MtrunA17_Chr4g0073741 68.352 613 169 6 1 596 1 605 0.0 856
Msa0352220 MtrunA17_Chr4g0031301 66.997 606 175 6 8 596 9 606 0.0 820
Msa0352220 MtrunA17_Chr4g0031321 66.667 606 177 6 8 596 9 606 0.0 815
Msa0352220 MtrunA17_Chr7g0223181 66.227 607 180 6 8 597 9 607 0.0 810
Msa0352220 MtrunA17_Chr7g0262821 65.842 606 182 6 8 596 9 606 0.0 806
Msa0352220 MtrunA17_Chr7g0223291 66.172 606 180 6 8 596 9 606 0.0 805
Msa0352220 MtrunA17_Chr6g0462681 55.167 600 243 6 8 590 9 599 0.0 665
Msa0352220 MtrunA17_Chr6g0462601 55.833 600 239 6 8 590 9 599 0.0 652
Msa0352220 MtrunA17_Chr8g0388671 55.593 599 235 8 2 581 32 618 0.0 652
Msa0352220 MtrunA17_Chr7g0223741 58.541 562 218 7 5 561 6 557 0.0 650
Msa0352220 MtrunA17_Chr8g0388761 54.849 598 239 8 3 581 33 618 0.0 649
Msa0352220 MtrunA17_Chr5g0427901 54.355 620 243 9 8 597 9 618 0.0 643
Msa0352220 MtrunA17_Chr7g0223751 56.940 562 226 9 8 564 9 559 0.0 639
Msa0352220 MtrunA17_Chr7g0223711 56.762 562 228 7 5 561 6 557 0.0 638
Msa0352220 MtrunA17_Chr7g0221661 54.844 609 246 9 8 597 9 607 0.0 638
Msa0352220 MtrunA17_Chr2g0298851 54.207 618 253 10 5 602 6 613 0.0 635
Msa0352220 MtrunA17_Chr2g0293581 52.932 614 260 9 7 600 9 613 0.0 628
Msa0352220 MtrunA17_Chr7g0233311 57.549 563 225 6 8 565 9 562 0.0 627
Msa0352220 MtrunA17_Chr8g0350731 57.245 559 223 8 8 561 37 584 0.0 626
Msa0352220 MtrunA17_Chr7g0223731 56.406 562 229 8 5 561 6 556 0.0 624
Msa0352220 MtrunA17_Chr6g0462771 52.231 605 262 7 5 589 6 603 0.0 619
Msa0352220 MtrunA17_Chr7g0223721 53.008 615 259 10 8 602 9 613 0.0 619
Msa0352220 MtrunA17_Chr7g0223801 52.381 609 259 10 8 597 9 605 0.0 617
Msa0352220 MtrunA17_Chr7g0223701 53.247 616 253 9 8 597 9 615 0.0 616
Msa0352220 MtrunA17_Chr7g0223781 55.654 566 234 8 8 568 9 562 0.0 616
Msa0352220 MtrunA17_Chr6g0462711 52.815 604 261 7 5 589 7 605 0.0 616
Msa0352220 MtrunA17_Chr6g0462641 52.467 608 265 7 1 589 1 603 0.0 614
Msa0352220 MtrunA17_Chr7g0223621 55.160 562 236 8 8 564 9 559 0.0 613
Msa0352220 MtrunA17_Chr7g0225671 53.376 622 259 11 1 602 9 619 0.0 613
Msa0352220 MtrunA17_Chr7g0223031 54.982 562 237 8 5 561 6 556 0.0 612
Msa0352220 MtrunA17_Chr2g0287631 52.520 615 263 9 8 602 9 614 0.0 607
Msa0352220 MtrunA17_Chr7g0223851 52.666 619 261 11 5 602 15 622 0.0 604
Msa0352220 MtrunA17_Chr7g0248131 55.923 574 227 12 8 573 9 564 0.0 604
Msa0352220 MtrunA17_Chr2g0298861 51.597 626 261 12 9 602 10 625 0.0 603
Msa0352220 MtrunA17_Chr4g0040931 51.870 615 266 10 8 602 9 613 0.0 602
Msa0352220 MtrunA17_Chr7g0248151 55.893 560 226 9 8 559 9 555 0.0 589
Msa0352220 MtrunA17_Chr7g0223631 54.204 559 226 10 8 561 9 542 0.0 588
Msa0352220 MtrunA17_Chr7g0233301 54.626 562 216 6 8 567 9 533 0.0 584
Msa0352220 MtrunA17_Chr2g0296391 56.015 532 220 6 4 530 5 527 0.0 583
Msa0352220 MtrunA17_Chr7g0217971 51.082 601 240 10 8 589 9 574 0.0 574
Msa0352220 MtrunA17_Chr7g0223821 51.125 622 269 10 5 602 6 616 0.0 573
Msa0352220 MtrunA17_Chr7g0215261 52.920 565 215 8 7 565 8 527 0.0 557
Msa0352220 MtrunA17_Chr8g0390821 47.121 660 254 13 8 597 11 645 0.0 551
Msa0352220 MtrunA17_Chr6g0462611 52.277 549 234 7 59 590 10 547 0.0 551
Msa0352220 MtrunA17_Chr4g0012541 49.435 619 272 12 5 602 6 604 0.0 551
Msa0352220 MtrunA17_Chr7g0223831 52.473 566 226 9 5 565 6 533 0.0 548
Msa0352220 MtrunA17_Chr2g0331381 52.407 540 186 8 9 546 7 477 0.0 546
Msa0352220 MtrunA17_Chr7g0223541 54.125 497 215 6 71 564 31 517 0.0 523
Msa0352220 MtrunA17_Chr7g0228281 47.403 616 249 10 7 602 69 629 1.33e-179 522
Msa0352220 MtrunA17_Chr2g0293601 53.295 516 199 7 8 521 9 484 9.10e-179 514
Msa0352220 MtrunA17_Chr7g0223681 54.298 477 204 7 88 561 2 467 2.68e-175 508
Msa0352220 MtrunA17_Chr2g0299921 66.581 389 120 4 8 396 1029 1407 2.54e-168 523
Msa0352220 MtrunA17_Chr2g0299921 65.979 388 121 6 8 394 1413 1790 4.47e-155 488
Msa0352220 MtrunA17_Chr2g0299821 60.880 409 145 6 9 411 10 409 1.04e-166 484
Msa0352220 MtrunA17_Chr2g0299821 41.429 140 59 3 425 541 343 482 8.04e-22 99.0
Msa0352220 MtrunA17_Chr2g0293561 62.567 374 130 4 8 379 9 374 2.06e-165 477
Msa0352220 MtrunA17_Chr2g0299851 63.824 387 130 5 8 394 1038 1414 1.31e-162 501
Msa0352220 MtrunA17_Chr2g0279901 46.037 593 275 16 9 583 57 622 2.12e-161 477
Msa0352220 MtrunA17_Chr4g0049501 44.610 603 294 14 6 591 51 630 2.15e-157 467
Msa0352220 MtrunA17_Chr2g0299791 63.824 387 130 5 8 394 1042 1418 5.32e-157 486
Msa0352220 MtrunA17_Chr6g0462861 46.235 571 261 14 8 563 9 548 2.32e-153 456
Msa0352220 MtrunA17_Chr1g0166551 43.257 608 208 11 8 597 5 493 1.14e-150 443
Msa0352220 MtrunA17_Chr2g0299881 60.870 391 139 6 8 394 1038 1418 1.36e-147 462
Msa0352220 MtrunA17_Chr2g0299881 62.121 66 21 1 184 249 1414 1475 3.55e-16 82.8
Msa0352220 MtrunA17_Chr2g0277021 45.095 581 285 14 7 575 70 628 2.86e-146 439
Msa0352220 MtrunA17_Chr2g0293271 47.865 445 148 6 20 459 43 408 7.14e-131 389
Msa0352220 MtrunA17_Chr1g0151991 62.069 290 104 3 5 292 6 291 8.98e-125 369
Msa0352220 MtrunA17_Chr7g0245521 65.056 269 88 3 8 274 9 273 4.22e-119 354
Msa0352220 MtrunA17_Chr6g0462631 48.529 340 124 5 83 422 9 297 1.49e-96 297
Msa0352220 MtrunA17_Chr6g0462751 42.936 361 139 5 61 419 9 304 3.32e-90 281
Msa0352220 MtrunA17_Chr7g0223761 38.889 378 170 5 240 602 3 334 2.94e-80 256
Msa0352220 MtrunA17_Chr7g0223671 59.896 192 75 1 71 262 8 197 3.31e-78 246
Msa0352220 MtrunA17_Chr1g0188601 62.176 193 69 2 91 283 3 191 4.37e-77 243
Msa0352220 MtrunA17_Chr4g0004331 33.857 446 271 6 9 441 4 438 4.76e-75 256
Msa0352220 MtrunA17_Chr5g0394391 31.755 507 316 12 8 498 29 521 3.31e-74 248
Msa0352220 MtrunA17_Chr2g0331901 31.866 477 299 8 9 470 4 469 8.57e-74 253
Msa0352220 MtrunA17_Chr7g0245531 44.013 309 150 6 306 597 2 304 8.78e-73 236
Msa0352220 MtrunA17_Chr6g0462791 60.556 180 66 3 105 284 1 175 3.17e-68 219
Msa0352220 MtrunA17_Chr5g0444001 31.111 450 290 7 9 452 4 439 2.17e-66 226
Msa0352220 MtrunA17_Chr3g0098761 29.365 504 334 8 9 502 4 495 5.23e-66 230
Msa0352220 MtrunA17_Chr1g0152001 40.594 303 156 7 317 602 2 297 4.80e-65 216
Msa0352220 MtrunA17_Chr7g0223771 64.935 154 52 1 91 244 2 153 1.02e-64 209
Msa0352220 MtrunA17_Chr4g0060241 30.315 508 316 12 9 500 21 506 1.82e-57 202
Msa0352220 MtrunA17_Chr4g0064621 31.879 527 326 13 8 512 140 655 3.26e-57 204
Msa0352220 MtrunA17_Chr1g0188611 47.347 245 120 5 323 564 1 239 1.65e-55 189
Msa0352220 MtrunA17_Chr7g0223861 65.414 133 44 1 75 207 4 134 2.44e-54 181
Msa0352220 MtrunA17_Chr3g0120101 26.554 531 346 11 6 502 24 544 1.58e-51 190
Msa0352220 MtrunA17_Chr4g0046881 61.074 149 51 3 128 276 10 151 1.58e-51 174
Msa0352220 MtrunA17_Chr6g0462701 52.353 170 53 3 102 271 27 168 2.72e-46 160
Msa0352220 MtrunA17_Chr5g0394231 40.693 231 82 5 71 300 5 181 1.87e-45 159
Msa0352220 MtrunA17_Chr5g0442171 32.845 341 189 11 169 491 1 319 1.63e-43 158
Msa0352220 MtrunA17_Chr6g0462691 52.055 146 63 2 323 465 1 142 2.47e-43 152
Msa0352220 MtrunA17_Chr5g0394221 35.556 270 160 6 299 563 68 328 5.82e-42 155
Msa0352220 MtrunA17_Chr6g0481391 53.226 124 53 2 35 158 13 131 1.12e-34 127
Msa0352220 MtrunA17_Chr2g0284661 62.500 96 33 1 360 452 48 143 4.62e-30 115
Msa0352220 MtrunA17_Chr2g0284661 83.636 55 9 0 224 278 5 59 9.63e-24 97.4
Msa0352220 MtrunA17_Chr4g0046871 54.717 106 43 2 336 441 5 105 7.54e-30 113
Msa0352220 MtrunA17_Chr6g0462621 70.423 71 20 1 1 70 1 71 2.88e-28 107
Msa0352220 MtrunA17_Chr7g0245541 34.896 192 107 3 423 597 1 191 1.77e-27 110
Msa0352220 MtrunA17_Chr6g0481381 63.514 74 27 0 175 248 1 74 2.34e-25 103
Msa0352220 MtrunA17_Chr7g0223691 70.769 65 17 1 8 70 9 73 4.12e-25 99.0
Msa0352220 MtrunA17_Chr1g0188591 64.706 68 22 1 5 70 6 73 8.13e-24 95.9
Msa0352220 MtrunA17_Chr7g0223791 52.439 82 31 2 53 134 18 91 2.37e-19 86.3
Msa0352220 MtrunA17_Chr7g0223791 37.975 79 49 0 486 564 90 168 3.34e-12 65.5
Msa0352220 MtrunA17_Chr7g0223881 50.667 75 36 1 289 363 9 82 4.30e-18 79.3
Msa0352220 MtrunA17_Chr6g0462801 53.030 66 30 1 323 387 1 66 6.72e-16 72.8
Msa0352220 MtrunA17_Chr7g0221651 40.698 86 49 1 214 299 1 84 6.35e-15 70.5
Msa0352220 MtrunA17_Chr4g0064651 38.462 91 53 1 404 491 30 120 1.25e-14 70.9
Msa0352220 MtrunA17_Chr2g0299891 65.909 44 15 0 317 360 2 45 5.61e-14 67.4
Msa0352220 MtrunA17_Chr5g0442161 30.065 153 100 4 8 156 28 177 6.38e-13 67.4
Msa0352220 MtrunA17_Chr2g0299941 60.000 55 19 1 405 456 23 77 1.14e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0352220 AT1G16030.1 67.047 613 177 6 1 596 1 605 0.0 832
Msa0352220 AT3G12580.1 67.051 607 175 6 8 597 9 607 0.0 814
Msa0352220 AT5G02500.1 66.227 607 180 6 8 597 9 607 0.0 808
Msa0352220 AT3G09440.1 66.063 607 181 6 8 597 9 607 0.0 800
Msa0352220 AT3G09440.4 66.063 607 181 6 8 597 9 607 0.0 800
Msa0352220 AT3G09440.2 66.063 607 181 6 8 597 9 607 0.0 800
Msa0352220 AT3G09440.3 66.063 607 181 6 8 597 9 607 0.0 800
Msa0352220 AT5G02490.1 65.404 607 185 6 8 597 9 607 0.0 795
Msa0352220 AT1G56410.1 65.124 605 188 7 8 597 9 605 0.0 787
Msa0352220 AT5G42020.1 55.863 614 240 8 2 596 31 632 0.0 672
Msa0352220 AT5G42020.3 55.863 614 240 8 2 596 36 637 0.0 672
Msa0352220 AT5G28540.1 55.700 614 241 8 2 596 31 632 0.0 669
Msa0352220 AT1G09080.2 55.776 606 238 7 9 596 43 636 0.0 651
Msa0352220 AT1G09080.1 55.776 606 238 7 9 596 53 646 0.0 651
Msa0352220 AT5G42020.2 52.000 600 230 11 2 596 31 577 0.0 585
Msa0352220 AT5G02500.2 70.558 394 108 4 8 401 9 394 0.0 571
Msa0352220 AT5G02500.2 45.652 92 36 1 520 597 386 477 8.66e-16 80.9
Msa0352220 AT5G09590.1 45.983 585 277 15 9 577 60 621 1.60e-156 465
Msa0352220 AT4G37910.1 46.316 570 268 13 9 563 55 601 2.15e-151 452
Msa0352220 AT4G37910.2 46.316 570 268 13 9 563 55 601 2.15e-151 452
Msa0352220 AT5G49910.1 44.234 581 290 14 7 575 79 637 1.19e-144 436
Msa0352220 AT4G24280.1 43.688 602 297 15 7 599 79 647 2.40e-144 435
Msa0352220 AT1G79930.2 34.081 446 270 6 9 441 4 438 1.35e-78 265
Msa0352220 AT1G79930.1 34.081 446 270 6 9 441 4 438 2.68e-78 265
Msa0352220 AT1G79920.1 34.081 446 270 6 9 441 4 438 7.55e-78 261
Msa0352220 AT1G79920.3 34.081 446 270 6 9 441 4 438 1.01e-77 261
Msa0352220 AT1G79920.2 34.081 446 270 6 9 441 4 438 1.01e-77 261
Msa0352220 AT1G79920.4 34.081 446 270 6 9 441 4 438 1.01e-77 261
Msa0352220 AT1G11660.4 28.418 651 391 16 9 602 4 636 4.75e-73 249
Msa0352220 AT1G11660.3 28.418 651 391 16 9 602 4 636 4.75e-73 249
Msa0352220 AT1G11660.1 28.418 651 391 16 9 602 4 636 4.75e-73 249
Msa0352220 AT2G32120.1 30.077 522 322 13 8 512 30 525 2.94e-65 224
Msa0352220 AT2G32120.2 30.077 522 322 13 8 512 30 525 2.94e-65 224
Msa0352220 AT1G11660.2 28.247 616 367 16 44 602 2 599 4.14e-64 224
Msa0352220 AT4G16660.1 26.154 455 303 8 5 436 23 467 4.54e-48 181
Msa0352220 AT4G16660.2 26.154 455 303 8 5 436 47 491 4.96e-48 181

Find 123 sgRNAs with CRISPR-Local

Find 294 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAAATTGCTGAAACTTATT+TGG 0.082085 3_1:-20601714 Msa0352220:CDS
CATGGTGTTGTCTAAATTAA+AGG 0.157364 3_1:-20601735 Msa0352220:CDS
TCAAAACAAATATTGATATT+TGG 0.181030 3_1:+20600663 None:intergenic
TTGATATTTGGAACCTTTCT+AGG 0.182799 3_1:+20600675 None:intergenic
ACTGCAGCTGCTATTGCTTA+TGG 0.208608 3_1:-20601563 Msa0352220:CDS
TGGTTTGGAACAACCTTAAA+AGG 0.212043 3_1:+20601830 None:intergenic
TGTTGATGTTAATGGAATTT+TGG 0.222928 3_1:-20600640 Msa0352220:CDS
GTTTCAACACCAAGACTAAA+AGG 0.273894 3_1:+20600876 None:intergenic
CAATCATGGGTTTGTCTTTG+TGG 0.290878 3_1:+20601810 None:intergenic
CAGTGAGAATTCCAAAGCTT+TGG 0.318063 3_1:-20601321 Msa0352220:CDS
AGAAGCAGGAGTTGGAAAAT+AGG 0.326412 3_1:-20600335 Msa0352220:CDS
CCTGATGAAGCTGTGGCTTA+TGG 0.330474 3_1:-20600975 Msa0352220:CDS
AATGTTCTTGTCTTTGATCT+TGG 0.331932 3_1:-20601506 Msa0352220:CDS
AGTGAGAATTCCAAAGCTTT+GGG 0.334628 3_1:-20601320 Msa0352220:CDS
GAAATTGCTGAAACTTATTT+GGG 0.342870 3_1:-20601713 Msa0352220:CDS
ACACGGTTCCCTTGGTCGTT+TGG 0.354947 3_1:+20602013 None:intergenic
CGAAGGAATGTTCCAGGTTA+AGG 0.357597 3_1:-20601447 Msa0352220:CDS
GAGGGATGCCGAGCGGTATA+AGG 0.359508 3_1:-20600520 Msa0352220:CDS
TTCAGTAGTTGTTGAATCTT+CGG 0.360449 3_1:+20601050 None:intergenic
ACTTTGGGAGATACTCATTT+GGG 0.360641 3_1:-20601422 Msa0352220:CDS
TTCCAGGTTAAGGCCACTTT+GGG 0.364020 3_1:-20601437 Msa0352220:CDS
ACCGATACTGAAAGATTAAT+AGG 0.373414 3_1:-20601968 Msa0352220:CDS
TTATGTCTTGTTGGACTGAT+TGG 0.374106 3_1:+20601861 None:intergenic
AGCTGCTATTGCTTATGGTT+TGG 0.376546 3_1:-20601558 Msa0352220:CDS
CACTTTGGGAGATACTCATT+TGG 0.379211 3_1:-20601423 Msa0352220:CDS
ATGGGTTTGTCTTTGTGGTT+TGG 0.379293 3_1:+20601815 None:intergenic
ACTCATCAATTTCTGCCAAT+TGG 0.383486 3_1:+20600367 None:intergenic
TCCAACAAGACATAAGGTTT+TGG 0.384795 3_1:-20601853 Msa0352220:CDS
AGGCAGAGGATGAGGAGGTT+AGG 0.400721 3_1:-20600500 Msa0352220:CDS
TCTTAACCTCCCCAAAGCTT+TGG 0.400779 3_1:+20601310 None:intergenic
ACTCAACCTTCCCTCCTTGT+TGG 0.402188 3_1:+20600566 None:intergenic
CAATAACAAATTCATGAACT+TGG 0.402514 3_1:+20601093 None:intergenic
CTTGTCTTTGATCTTGGTGG+TGG 0.402810 3_1:-20601500 Msa0352220:CDS
GCCAAAACCTTATGTCTTGT+TGG 0.404961 3_1:+20601852 None:intergenic
GAAGCAGGAGTTGGAAAATA+GGG 0.411652 3_1:-20600334 Msa0352220:CDS
TTTGATGCCAAACGTTTGAT+TGG 0.412646 3_1:-20601899 Msa0352220:CDS
GTTCTTGTCTTTGATCTTGG+TGG 0.414303 3_1:-20601503 Msa0352220:CDS
GTTCATGAATTTGTTATTGT+CGG 0.430114 3_1:-20601089 Msa0352220:CDS
TTTGATCAAAACTTTACCTT+GGG 0.431046 3_1:+20600776 None:intergenic
AGAATTATGTTTATGAAATG+AGG 0.432257 3_1:-20600449 Msa0352220:CDS
GTAGGGATCCTAGTATTCTT+CGG 0.433514 3_1:+20600825 None:intergenic
ATGAAATGAGGGAGAGAGTG+AGG 0.434187 3_1:-20600437 Msa0352220:CDS
TTTGTAATTGACAACAATCA+TGG 0.440479 3_1:+20601796 None:intergenic
CTCCCAAAGTGGCCTTAACC+TGG 0.442345 3_1:+20601435 None:intergenic
TGTTGACGAAGGAATGTTCC+AGG 0.444778 3_1:-20601453 Msa0352220:CDS
GTTCCAGGTTAAGGCCACTT+TGG 0.448544 3_1:-20601438 Msa0352220:CDS
TCGGTGAAGGCAACGTAAGA+AGG 0.448873 3_1:+20601986 None:intergenic
GGATGAGGAGGTTAGGAAGA+AGG 0.449706 3_1:-20600493 Msa0352220:CDS
GATTGAAGGCTTATTGCTGT+TGG 0.451601 3_1:-20600907 Msa0352220:CDS
ATGCTCTTTAGTACCTAGAA+AGG 0.452071 3_1:-20600688 Msa0352220:CDS
CCATAAGCCACAGCTTCATC+AGG 0.453102 3_1:+20600975 None:intergenic
ATCATTCTTCTCATCTCTTG+AGG 0.461303 3_1:+20600543 None:intergenic
AGTCTTGGTGTTGAAACTAA+AGG 0.463181 3_1:-20600870 Msa0352220:CDS
TATGAAATAATTCAACAAGA+TGG 0.465475 3_1:+20601369 None:intergenic
ACTGCTGAAGATAAGACAAA+AGG 0.472237 3_1:-20600612 Msa0352220:CDS
ACCTATTAATCTTTCAGTAT+CGG 0.473250 3_1:+20601967 None:intergenic
GCGGTATAAGGCAGAGGATG+AGG 0.474792 3_1:-20600508 Msa0352220:CDS
CTTTGATCAAAACTTTACCT+TGG 0.475667 3_1:+20600775 None:intergenic
GCGATGACCAATCAAACGTT+TGG 0.484985 3_1:+20601892 None:intergenic
AGGAGAACATGCTAAGACTA+AGG 0.487241 3_1:-20600745 Msa0352220:CDS
GAGGAAGCTTGAAAAGGTAG+TGG 0.489230 3_1:-20600418 Msa0352220:CDS
GAAGGAGACAAGAAGATTGA+AGG 0.489825 3_1:-20600921 Msa0352220:CDS
TGAGTTTGAATTCAAGAAGC+AGG 0.491801 3_1:-20600349 Msa0352220:CDS
TGAATTCAAGAAGCAGGAGT+TGG 0.497584 3_1:-20600343 Msa0352220:CDS
GATCCTCATCACATTAAAAC+CGG 0.502743 3_1:+20601598 None:intergenic
GAGAGTGAGGAAGCTTGAAA+AGG 0.502774 3_1:-20600424 Msa0352220:CDS
GAATTATGTTTATGAAATGA+GGG 0.504803 3_1:-20600448 Msa0352220:CDS
ATGGCTTGACAGAAACCAAT+TGG 0.505887 3_1:-20600382 Msa0352220:CDS
CTGCTGAAGATAAGACAAAA+GGG 0.507869 3_1:-20600611 Msa0352220:CDS
AATCAGTCCAACAAGACATA+AGG 0.516258 3_1:-20601859 Msa0352220:CDS
AAGGGGTGACACGGTTCCCT+TGG 0.517703 3_1:+20602005 None:intergenic
TGTACCAAGGTCAATGCCTA+TGG 0.520221 3_1:+20602084 None:intergenic
CATGAATTTGTTATTGTCGG+AGG 0.522698 3_1:-20601086 Msa0352220:CDS
TGTGAGTTGTTGAAGTAAGC+AGG 0.526143 3_1:+20601653 None:intergenic
TTGTAATTGACAACAATCAT+GGG 0.527199 3_1:+20601797 None:intergenic
CGGTGAAGGCAACGTAAGAA+GGG 0.529846 3_1:+20601987 None:intergenic
AGCTTGATTCACTATGTGAA+GGG 0.532350 3_1:-20601232 Msa0352220:CDS
CAAAACTTTACCTTGGGCTG+TGG 0.533300 3_1:+20600782 None:intergenic
TAGTGGAGGAAAGTATAGAA+TGG 0.539580 3_1:-20600401 Msa0352220:CDS
TCCTTCGTCAACAGTCACTA+AGG 0.546693 3_1:+20601463 None:intergenic
TGCCGAGCGGTATAAGGCAG+AGG 0.550389 3_1:-20600514 Msa0352220:CDS
ACCTCAAGAGATATCTTCCA+TGG 0.553297 3_1:-20601753 Msa0352220:CDS
ATGAATTTGTTATTGTCGGA+GGG 0.553906 3_1:-20601085 Msa0352220:CDS
ATTTCATAGAAAGTACAAGA+AGG 0.557642 3_1:-20601354 Msa0352220:CDS
ATCCTCTGCCTTATACCGCT+CGG 0.563812 3_1:+20600512 None:intergenic
GCAGCAATATTGAATACTGA+AGG 0.571285 3_1:-20600939 Msa0352220:CDS
TTGGGAGATACTCATTTGGG+TGG 0.571754 3_1:-20601419 Msa0352220:CDS
GAAGATTCAACAACTACTGA+AGG 0.574421 3_1:-20601048 Msa0352220:CDS
GACAAGAAAATGTGGAGAGA+AGG 0.578701 3_1:-20601536 Msa0352220:CDS
ATGATTGTTGTCAATTACAA+AGG 0.584826 3_1:-20601794 Msa0352220:CDS
TAATCTTTCAGTATCGGTGA+AGG 0.586135 3_1:+20601973 None:intergenic
AGACAAGCTACAAAAGATGC+AGG 0.588448 3_1:-20601629 Msa0352220:CDS
ATCATTATCACCAACAAGGA+GGG 0.590966 3_1:-20600576 Msa0352220:CDS
AGAGATGAGAAGAATGATGA+GGG 0.595789 3_1:-20600538 Msa0352220:CDS
GAAGCTTGAAAAGGTAGTGG+AGG 0.595927 3_1:-20600415 Msa0352220:CDS
AAGGAAATGTTTAGTGTCAA+CGG 0.596200 3_1:-20601029 Msa0352220:CDS
TGTGGAGAAGTGTTTGATTG+AGG 0.604035 3_1:-20601132 Msa0352220:CDS
TACAGCTGTAGCTTGTACCA+AGG 0.607846 3_1:+20602071 None:intergenic
AGAATTCCAAAGCTTTGGGG+AGG 0.611200 3_1:-20601316 Msa0352220:CDS
CATTAATCCTGATGAAGCTG+TGG 0.613030 3_1:-20600982 Msa0352220:CDS
TAAGCAATAGCAGCTGCAGT+AGG 0.618882 3_1:+20601566 None:intergenic
GTGAGAATTCCAAAGCTTTG+GGG 0.624343 3_1:-20601319 Msa0352220:CDS
AAGAGATGAGAAGAATGATG+AGG 0.628540 3_1:-20600539 Msa0352220:CDS
CAACGTAAGAAGGGGTGACA+CGG 0.629570 3_1:+20601996 None:intergenic
AGATCATTCCAAACGACCAA+GGG 0.630317 3_1:-20602021 Msa0352220:CDS
GAGATCATTCCAAACGACCA+AGG 0.630381 3_1:-20602022 Msa0352220:CDS
ATGGTTTGGACAAGAAAATG+TGG 0.630603 3_1:-20601544 Msa0352220:CDS
TTATCACCAACAAGGAGGGA+AGG 0.634341 3_1:-20600572 Msa0352220:CDS
GAGCTTGATTCACTATGTGA+AGG 0.634799 3_1:-20601233 Msa0352220:CDS
CAAATGAGTATCTCCCAAAG+TGG 0.637746 3_1:+20601424 None:intergenic
AAAGCCATAGGCATTGACCT+TGG 0.638972 3_1:-20602088 Msa0352220:CDS
TCCTTAGTGACTGTTGACGA+AGG 0.640013 3_1:-20601464 Msa0352220:CDS
ACCATGGAAGATATCTCTTG+AGG 0.641800 3_1:+20601752 None:intergenic
GTATAAGGCAGAGGATGAGG+AGG 0.650304 3_1:-20600505 Msa0352220:CDS
CAGCATGCGAGAAAGCAAAG+AGG 0.658762 3_1:-20601286 Msa0352220:CDS
GATCATTATCACCAACAAGG+AGG 0.670278 3_1:-20600577 Msa0352220:CDS
GGTGAAGGCAACGTAAGAAG+GGG 0.671684 3_1:+20601988 None:intergenic
CTTTAATTTAGACAACACCA+TGG 0.682894 3_1:+20601736 None:intergenic
CAGAAAGTGCATGAAGACTG+TGG 0.701540 3_1:-20601150 Msa0352220:CDS
CTTGGTGTTGAAACTAAAGG+TGG 0.707841 3_1:-20600867 Msa0352220:CDS
GAATGATGAGGGATGCCGAG+CGG 0.719182 3_1:-20600527 Msa0352220:CDS
GAAGATCATTATCACCAACA+AGG 0.723817 3_1:-20600580 Msa0352220:CDS
GCTACAGCTGTATTGCAGTG+TGG 0.732257 3_1:-20602060 Msa0352220:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCAAAACAAATATTGATATT+TGG + chr3_1:20601766-20601785 None:intergenic 15.0%
!! TCAAAACAAATATTGATATT+TGG + chr3_1:20601766-20601785 None:intergenic 15.0%
!!! TGTTGATTTTGATAACAATT+TGG - chr3_1:20601030-20601049 Msa0352220:CDS 20.0%
!! TATGAAATAATTCAACAAGA+TGG + chr3_1:20601060-20601079 None:intergenic 20.0%
!!! TTTTTTTGAGGAAATAAACA+AGG - chr3_1:20601246-20601265 Msa0352220:CDS 20.0%
!! AGAATTATGTTTATGAAATG+AGG - chr3_1:20601977-20601996 Msa0352220:CDS 20.0%
!! GAATTATGTTTATGAAATGA+GGG - chr3_1:20601978-20601997 Msa0352220:CDS 20.0%
!!! TGTTGATTTTGATAACAATT+TGG - chr3_1:20601030-20601049 Msa0352220:CDS 20.0%
!! TATGAAATAATTCAACAAGA+TGG + chr3_1:20601060-20601079 None:intergenic 20.0%
!!! TTTTTTTGAGGAAATAAACA+AGG - chr3_1:20601246-20601265 Msa0352220:CDS 20.0%
!! AGAATTATGTTTATGAAATG+AGG - chr3_1:20601977-20601996 Msa0352220:CDS 20.0%
!! GAATTATGTTTATGAAATGA+GGG - chr3_1:20601978-20601997 Msa0352220:CDS 20.0%
! ACCTATTAATCTTTCAGTAT+CGG + chr3_1:20600462-20600481 None:intergenic 25.0%
! TTGTAATTGACAACAATCAT+GGG + chr3_1:20600632-20600651 None:intergenic 25.0%
! TTTGTAATTGACAACAATCA+TGG + chr3_1:20600633-20600652 None:intergenic 25.0%
! ATGATTGTTGTCAATTACAA+AGG - chr3_1:20600632-20600651 Msa0352220:CDS 25.0%
! GAAATTGCTGAAACTTATTT+GGG - chr3_1:20600713-20600732 Msa0352220:CDS 25.0%
!! ATTTCATAGAAAGTACAAGA+AGG - chr3_1:20601072-20601091 Msa0352220:CDS 25.0%
! CAATAACAAATTCATGAACT+TGG + chr3_1:20601336-20601355 None:intergenic 25.0%
!!! GTTCATGAATTTGTTATTGT+CGG - chr3_1:20601337-20601356 Msa0352220:CDS 25.0%
!!! AAACATTCTCCTTTTTTGTA+GGG + chr3_1:20601621-20601640 None:intergenic 25.0%
!!! AAAACATTCTCCTTTTTTGT+AGG + chr3_1:20601622-20601641 None:intergenic 25.0%
! TTTGATCAAAACTTTACCTT+GGG + chr3_1:20601653-20601672 None:intergenic 25.0%
!!! TGTTTTGATGTTGATGTTAA+TGG - chr3_1:20601778-20601797 Msa0352220:CDS 25.0%
!! TGTTGATGTTAATGGAATTT+TGG - chr3_1:20601786-20601805 Msa0352220:CDS 25.0%
! ACCTATTAATCTTTCAGTAT+CGG + chr3_1:20600462-20600481 None:intergenic 25.0%
! TTGTAATTGACAACAATCAT+GGG + chr3_1:20600632-20600651 None:intergenic 25.0%
! TTTGTAATTGACAACAATCA+TGG + chr3_1:20600633-20600652 None:intergenic 25.0%
! ATGATTGTTGTCAATTACAA+AGG - chr3_1:20600632-20600651 Msa0352220:CDS 25.0%
! GAAATTGCTGAAACTTATTT+GGG - chr3_1:20600713-20600732 Msa0352220:CDS 25.0%
!! ATTTCATAGAAAGTACAAGA+AGG - chr3_1:20601072-20601091 Msa0352220:CDS 25.0%
! CAATAACAAATTCATGAACT+TGG + chr3_1:20601336-20601355 None:intergenic 25.0%
!!! GTTCATGAATTTGTTATTGT+CGG - chr3_1:20601337-20601356 Msa0352220:CDS 25.0%
!!! AAACATTCTCCTTTTTTGTA+GGG + chr3_1:20601621-20601640 None:intergenic 25.0%
!!! AAAACATTCTCCTTTTTTGT+AGG + chr3_1:20601622-20601641 None:intergenic 25.0%
! TTTGATCAAAACTTTACCTT+GGG + chr3_1:20601653-20601672 None:intergenic 25.0%
!!! TGTTTTGATGTTGATGTTAA+TGG - chr3_1:20601778-20601797 Msa0352220:CDS 25.0%
!! TGTTGATGTTAATGGAATTT+TGG - chr3_1:20601786-20601805 Msa0352220:CDS 25.0%
ACCGATACTGAAAGATTAAT+AGG - chr3_1:20600458-20600477 Msa0352220:CDS 30.0%
!!! ATTTTTGGCTGATTGGTTTA+TGG + chr3_1:20600492-20600511 None:intergenic 30.0%
! GAGGTGAAAAATGTTTTTCT+TGG + chr3_1:20600658-20600677 None:intergenic 30.0%
! CTTTAATTTAGACAACACCA+TGG + chr3_1:20600693-20600712 None:intergenic 30.0%
!! CATGGTGTTGTCTAAATTAA+AGG - chr3_1:20600691-20600710 Msa0352220:CDS 30.0%
GGAAATTGCTGAAACTTATT+TGG - chr3_1:20600712-20600731 Msa0352220:CDS 30.0%
AATGTTCTTGTCTTTGATCT+TGG - chr3_1:20600920-20600939 Msa0352220:CDS 30.0%
!!! TGTTACTAGAGCTTTTTTTG+AGG - chr3_1:20601234-20601253 Msa0352220:CDS 30.0%
!! ATGAATTTGTTATTGTCGGA+GGG - chr3_1:20601341-20601360 Msa0352220:CDS 30.0%
TTCAGTAGTTGTTGAATCTT+CGG + chr3_1:20601379-20601398 None:intergenic 30.0%
AAGGAAATGTTTAGTGTCAA+CGG - chr3_1:20601397-20601416 Msa0352220:CDS 30.0%
!! TTTTGATTCCGAAGAATACT+AGG - chr3_1:20601593-20601612 Msa0352220:CDS 30.0%
CTTTGATCAAAACTTTACCT+TGG + chr3_1:20601654-20601673 None:intergenic 30.0%
!! GTTTTGATCAAAGTCTATGA+AGG - chr3_1:20601661-20601680 Msa0352220:CDS 30.0%
! TTGATATTTGGAACCTTTCT+AGG + chr3_1:20601754-20601773 None:intergenic 30.0%
! TGATGTTAATGGAATTTTGG+AGG - chr3_1:20601789-20601808 Msa0352220:CDS 30.0%
ACCGATACTGAAAGATTAAT+AGG - chr3_1:20600458-20600477 Msa0352220:CDS 30.0%
!!! ATTTTTGGCTGATTGGTTTA+TGG + chr3_1:20600492-20600511 None:intergenic 30.0%
! GAGGTGAAAAATGTTTTTCT+TGG + chr3_1:20600658-20600677 None:intergenic 30.0%
! CTTTAATTTAGACAACACCA+TGG + chr3_1:20600693-20600712 None:intergenic 30.0%
!! CATGGTGTTGTCTAAATTAA+AGG - chr3_1:20600691-20600710 Msa0352220:CDS 30.0%
GGAAATTGCTGAAACTTATT+TGG - chr3_1:20600712-20600731 Msa0352220:CDS 30.0%
AATGTTCTTGTCTTTGATCT+TGG - chr3_1:20600920-20600939 Msa0352220:CDS 30.0%
!!! TGTTACTAGAGCTTTTTTTG+AGG - chr3_1:20601234-20601253 Msa0352220:CDS 30.0%
!! ATGAATTTGTTATTGTCGGA+GGG - chr3_1:20601341-20601360 Msa0352220:CDS 30.0%
TTCAGTAGTTGTTGAATCTT+CGG + chr3_1:20601379-20601398 None:intergenic 30.0%
AAGGAAATGTTTAGTGTCAA+CGG - chr3_1:20601397-20601416 Msa0352220:CDS 30.0%
!! TTTTGATTCCGAAGAATACT+AGG - chr3_1:20601593-20601612 Msa0352220:CDS 30.0%
CTTTGATCAAAACTTTACCT+TGG + chr3_1:20601654-20601673 None:intergenic 30.0%
!! GTTTTGATCAAAGTCTATGA+AGG - chr3_1:20601661-20601680 Msa0352220:CDS 30.0%
! TTGATATTTGGAACCTTTCT+AGG + chr3_1:20601754-20601773 None:intergenic 30.0%
! TGATGTTAATGGAATTTTGG+AGG - chr3_1:20601789-20601808 Msa0352220:CDS 30.0%
ACAAGAAAAAGCAAAGCCAT+AGG - chr3_1:20600326-20600345 Msa0352220:CDS 35.0%
!! TAATCTTTCAGTATCGGTGA+AGG + chr3_1:20600456-20600475 None:intergenic 35.0%
CAAACGTTTGGCATCAAAAA+CGG + chr3_1:20600525-20600544 None:intergenic 35.0%
TTTGATGCCAAACGTTTGAT+TGG - chr3_1:20600527-20600546 Msa0352220:CDS 35.0%
! TTATGTCTTGTTGGACTGAT+TGG + chr3_1:20600568-20600587 None:intergenic 35.0%
AATCAGTCCAACAAGACATA+AGG - chr3_1:20600567-20600586 Msa0352220:CDS 35.0%
! TCCAACAAGACATAAGGTTT+TGG - chr3_1:20600573-20600592 Msa0352220:CDS 35.0%
!! CATAAGGTTTTGGCCTTTTA+AGG - chr3_1:20600583-20600602 Msa0352220:CDS 35.0%
! TGGTTTGGAACAACCTTAAA+AGG + chr3_1:20600599-20600618 None:intergenic 35.0%
AAAGATGCAGGAAAAATTGC+CGG - chr3_1:20600809-20600828 Msa0352220:CDS 35.0%
GATCCTCATCACATTAAAAC+CGG + chr3_1:20600831-20600850 None:intergenic 35.0%
! ATGGTTTGGACAAGAAAATG+TGG - chr3_1:20600882-20600901 Msa0352220:CDS 35.0%
! ACTTTGGGAGATACTCATTT+GGG - chr3_1:20601004-20601023 Msa0352220:CDS 35.0%
AGTGAGAATTCCAAAGCTTT+GGG - chr3_1:20601106-20601125 Msa0352220:CDS 35.0%
AGCTTGATTCACTATGTGAA+GGG - chr3_1:20601194-20601213 Msa0352220:CDS 35.0%
!! CATGAATTTGTTATTGTCGG+AGG - chr3_1:20601340-20601359 Msa0352220:CDS 35.0%
GAAGATTCAACAACTACTGA+AGG - chr3_1:20601378-20601397 Msa0352220:CDS 35.0%
! TCAGGATTAATGCTTTTGCA+AGG + chr3_1:20601436-20601455 None:intergenic 35.0%
GCAGCAATATTGAATACTGA+AGG - chr3_1:20601487-20601506 Msa0352220:CDS 35.0%
! GATGTTATGCCTTTTAGTCT+TGG - chr3_1:20601541-20601560 Msa0352220:CDS 35.0%
GTTTCAACACCAAGACTAAA+AGG + chr3_1:20601553-20601572 None:intergenic 35.0%
!! AGTCTTGGTGTTGAAACTAA+AGG - chr3_1:20601556-20601575 Msa0352220:CDS 35.0%
TACTAGGATCCCTACAAAAA+AGG - chr3_1:20601609-20601628 Msa0352220:CDS 35.0%
! ACTAAGGACAACTTTTTGCT+TGG - chr3_1:20601697-20601716 Msa0352220:CDS 35.0%
ATGCTCTTTAGTACCTAGAA+AGG - chr3_1:20601738-20601757 Msa0352220:CDS 35.0%
ACTGCTGAAGATAAGACAAA+AGG - chr3_1:20601814-20601833 Msa0352220:CDS 35.0%
CTGCTGAAGATAAGACAAAA+GGG - chr3_1:20601815-20601834 Msa0352220:CDS 35.0%
GAAGATCATTATCACCAACA+AGG - chr3_1:20601846-20601865 Msa0352220:CDS 35.0%
ATCATTATCACCAACAAGGA+GGG - chr3_1:20601850-20601869 Msa0352220:CDS 35.0%
ATCATTCTTCTCATCTCTTG+AGG + chr3_1:20601886-20601905 None:intergenic 35.0%
AAGAGATGAGAAGAATGATG+AGG - chr3_1:20601887-20601906 Msa0352220:CDS 35.0%
AGAGATGAGAAGAATGATGA+GGG - chr3_1:20601888-20601907 Msa0352220:CDS 35.0%
! TAGTGGAGGAAAGTATAGAA+TGG - chr3_1:20602025-20602044 Msa0352220:CDS 35.0%
ACTCATCAATTTCTGCCAAT+TGG + chr3_1:20602062-20602081 None:intergenic 35.0%
TGAGTTTGAATTCAAGAAGC+AGG - chr3_1:20602077-20602096 Msa0352220:CDS 35.0%
ACAAGAAAAAGCAAAGCCAT+AGG - chr3_1:20600326-20600345 Msa0352220:CDS 35.0%
!! TAATCTTTCAGTATCGGTGA+AGG + chr3_1:20600456-20600475 None:intergenic 35.0%
CAAACGTTTGGCATCAAAAA+CGG + chr3_1:20600525-20600544 None:intergenic 35.0%
TTTGATGCCAAACGTTTGAT+TGG - chr3_1:20600527-20600546 Msa0352220:CDS 35.0%
! TTATGTCTTGTTGGACTGAT+TGG + chr3_1:20600568-20600587 None:intergenic 35.0%
AATCAGTCCAACAAGACATA+AGG - chr3_1:20600567-20600586 Msa0352220:CDS 35.0%
! TCCAACAAGACATAAGGTTT+TGG - chr3_1:20600573-20600592 Msa0352220:CDS 35.0%
!! CATAAGGTTTTGGCCTTTTA+AGG - chr3_1:20600583-20600602 Msa0352220:CDS 35.0%
! TGGTTTGGAACAACCTTAAA+AGG + chr3_1:20600599-20600618 None:intergenic 35.0%
AAAGATGCAGGAAAAATTGC+CGG - chr3_1:20600809-20600828 Msa0352220:CDS 35.0%
GATCCTCATCACATTAAAAC+CGG + chr3_1:20600831-20600850 None:intergenic 35.0%
! ATGGTTTGGACAAGAAAATG+TGG - chr3_1:20600882-20600901 Msa0352220:CDS 35.0%
! ACTTTGGGAGATACTCATTT+GGG - chr3_1:20601004-20601023 Msa0352220:CDS 35.0%
AGTGAGAATTCCAAAGCTTT+GGG - chr3_1:20601106-20601125 Msa0352220:CDS 35.0%
AGCTTGATTCACTATGTGAA+GGG - chr3_1:20601194-20601213 Msa0352220:CDS 35.0%
!! CATGAATTTGTTATTGTCGG+AGG - chr3_1:20601340-20601359 Msa0352220:CDS 35.0%
GAAGATTCAACAACTACTGA+AGG - chr3_1:20601378-20601397 Msa0352220:CDS 35.0%
! TCAGGATTAATGCTTTTGCA+AGG + chr3_1:20601436-20601455 None:intergenic 35.0%
GCAGCAATATTGAATACTGA+AGG - chr3_1:20601487-20601506 Msa0352220:CDS 35.0%
! GATGTTATGCCTTTTAGTCT+TGG - chr3_1:20601541-20601560 Msa0352220:CDS 35.0%
GTTTCAACACCAAGACTAAA+AGG + chr3_1:20601553-20601572 None:intergenic 35.0%
!! AGTCTTGGTGTTGAAACTAA+AGG - chr3_1:20601556-20601575 Msa0352220:CDS 35.0%
TACTAGGATCCCTACAAAAA+AGG - chr3_1:20601609-20601628 Msa0352220:CDS 35.0%
! ACTAAGGACAACTTTTTGCT+TGG - chr3_1:20601697-20601716 Msa0352220:CDS 35.0%
ATGCTCTTTAGTACCTAGAA+AGG - chr3_1:20601738-20601757 Msa0352220:CDS 35.0%
ACTGCTGAAGATAAGACAAA+AGG - chr3_1:20601814-20601833 Msa0352220:CDS 35.0%
CTGCTGAAGATAAGACAAAA+GGG - chr3_1:20601815-20601834 Msa0352220:CDS 35.0%
GAAGATCATTATCACCAACA+AGG - chr3_1:20601846-20601865 Msa0352220:CDS 35.0%
ATCATTATCACCAACAAGGA+GGG - chr3_1:20601850-20601869 Msa0352220:CDS 35.0%
ATCATTCTTCTCATCTCTTG+AGG + chr3_1:20601886-20601905 None:intergenic 35.0%
AAGAGATGAGAAGAATGATG+AGG - chr3_1:20601887-20601906 Msa0352220:CDS 35.0%
AGAGATGAGAAGAATGATGA+GGG - chr3_1:20601888-20601907 Msa0352220:CDS 35.0%
! TAGTGGAGGAAAGTATAGAA+TGG - chr3_1:20602025-20602044 Msa0352220:CDS 35.0%
ACTCATCAATTTCTGCCAAT+TGG + chr3_1:20602062-20602081 None:intergenic 35.0%
TGAGTTTGAATTCAAGAAGC+AGG - chr3_1:20602077-20602096 Msa0352220:CDS 35.0%
AGATCATTCCAAACGACCAA+GGG - chr3_1:20600405-20600424 Msa0352220:CDS 40.0%
!!! TGTGTGGATTTTTGGCTGAT+TGG + chr3_1:20600499-20600518 None:intergenic 40.0%
!!! AACGGTGTTGTGTGGATTTT+TGG + chr3_1:20600507-20600526 None:intergenic 40.0%
GCCAAAACCTTATGTCTTGT+TGG + chr3_1:20600577-20600596 None:intergenic 40.0%
ATGGGTTTGTCTTTGTGGTT+TGG + chr3_1:20600614-20600633 None:intergenic 40.0%
CAATCATGGGTTTGTCTTTG+TGG + chr3_1:20600619-20600638 None:intergenic 40.0%
ACCTCAAGAGATATCTTCCA+TGG - chr3_1:20600673-20600692 Msa0352220:CDS 40.0%
ACCATGGAAGATATCTCTTG+AGG + chr3_1:20600677-20600696 None:intergenic 40.0%
TGTGAGTTGTTGAAGTAAGC+AGG + chr3_1:20600776-20600795 None:intergenic 40.0%
AGACAAGCTACAAAAGATGC+AGG - chr3_1:20600797-20600816 Msa0352220:CDS 40.0%
!!! TTGCCGGTTTTAATGTGATG+AGG - chr3_1:20600825-20600844 Msa0352220:CDS 40.0%
AGCTGCTATTGCTTATGGTT+TGG - chr3_1:20600868-20600887 Msa0352220:CDS 40.0%
GACAAGAAAATGTGGAGAGA+AGG - chr3_1:20600890-20600909 Msa0352220:CDS 40.0%
GTTCTTGTCTTTGATCTTGG+TGG - chr3_1:20600923-20600942 Msa0352220:CDS 40.0%
CAAATGAGTATCTCCCAAAG+TGG + chr3_1:20601005-20601024 None:intergenic 40.0%
! CACTTTGGGAGATACTCATT+TGG - chr3_1:20601003-20601022 Msa0352220:CDS 40.0%
CAGTGAGAATTCCAAAGCTT+TGG - chr3_1:20601105-20601124 Msa0352220:CDS 40.0%
GTGAGAATTCCAAAGCTTTG+GGG - chr3_1:20601107-20601126 Msa0352220:CDS 40.0%
GAGCTTGATTCACTATGTGA+AGG - chr3_1:20601193-20601212 Msa0352220:CDS 40.0%
!! TGTGGAGAAGTGTTTGATTG+AGG - chr3_1:20601294-20601313 Msa0352220:CDS 40.0%
CATTAATCCTGATGAAGCTG+TGG - chr3_1:20601444-20601463 Msa0352220:CDS 40.0%
GAAGGAGACAAGAAGATTGA+AGG - chr3_1:20601505-20601524 Msa0352220:CDS 40.0%
GATTGAAGGCTTATTGCTGT+TGG - chr3_1:20601519-20601538 Msa0352220:CDS 40.0%
!! CTTGGTGTTGAAACTAAAGG+TGG - chr3_1:20601559-20601578 Msa0352220:CDS 40.0%
GTAGGGATCCTAGTATTCTT+CGG + chr3_1:20601604-20601623 None:intergenic 40.0%
AGGAGAACATGCTAAGACTA+AGG - chr3_1:20601681-20601700 Msa0352220:CDS 40.0%
GATCATTATCACCAACAAGG+AGG - chr3_1:20601849-20601868 Msa0352220:CDS 40.0%
ATGGCTTGACAGAAACCAAT+TGG - chr3_1:20602044-20602063 Msa0352220:CDS 40.0%
TGAATTCAAGAAGCAGGAGT+TGG - chr3_1:20602083-20602102 Msa0352220:CDS 40.0%
AGAAGCAGGAGTTGGAAAAT+AGG - chr3_1:20602091-20602110 Msa0352220:CDS 40.0%
GAAGCAGGAGTTGGAAAATA+GGG - chr3_1:20602092-20602111 Msa0352220:CDS 40.0%
AGATCATTCCAAACGACCAA+GGG - chr3_1:20600405-20600424 Msa0352220:CDS 40.0%
!!! TGTGTGGATTTTTGGCTGAT+TGG + chr3_1:20600499-20600518 None:intergenic 40.0%
!!! AACGGTGTTGTGTGGATTTT+TGG + chr3_1:20600507-20600526 None:intergenic 40.0%
GCCAAAACCTTATGTCTTGT+TGG + chr3_1:20600577-20600596 None:intergenic 40.0%
ATGGGTTTGTCTTTGTGGTT+TGG + chr3_1:20600614-20600633 None:intergenic 40.0%
CAATCATGGGTTTGTCTTTG+TGG + chr3_1:20600619-20600638 None:intergenic 40.0%
ACCTCAAGAGATATCTTCCA+TGG - chr3_1:20600673-20600692 Msa0352220:CDS 40.0%
ACCATGGAAGATATCTCTTG+AGG + chr3_1:20600677-20600696 None:intergenic 40.0%
TGTGAGTTGTTGAAGTAAGC+AGG + chr3_1:20600776-20600795 None:intergenic 40.0%
AGACAAGCTACAAAAGATGC+AGG - chr3_1:20600797-20600816 Msa0352220:CDS 40.0%
!!! TTGCCGGTTTTAATGTGATG+AGG - chr3_1:20600825-20600844 Msa0352220:CDS 40.0%
AGCTGCTATTGCTTATGGTT+TGG - chr3_1:20600868-20600887 Msa0352220:CDS 40.0%
GACAAGAAAATGTGGAGAGA+AGG - chr3_1:20600890-20600909 Msa0352220:CDS 40.0%
GTTCTTGTCTTTGATCTTGG+TGG - chr3_1:20600923-20600942 Msa0352220:CDS 40.0%
CAAATGAGTATCTCCCAAAG+TGG + chr3_1:20601005-20601024 None:intergenic 40.0%
! CACTTTGGGAGATACTCATT+TGG - chr3_1:20601003-20601022 Msa0352220:CDS 40.0%
CAGTGAGAATTCCAAAGCTT+TGG - chr3_1:20601105-20601124 Msa0352220:CDS 40.0%
GTGAGAATTCCAAAGCTTTG+GGG - chr3_1:20601107-20601126 Msa0352220:CDS 40.0%
GAGCTTGATTCACTATGTGA+AGG - chr3_1:20601193-20601212 Msa0352220:CDS 40.0%
!! TGTGGAGAAGTGTTTGATTG+AGG - chr3_1:20601294-20601313 Msa0352220:CDS 40.0%
CATTAATCCTGATGAAGCTG+TGG - chr3_1:20601444-20601463 Msa0352220:CDS 40.0%
GAAGGAGACAAGAAGATTGA+AGG - chr3_1:20601505-20601524 Msa0352220:CDS 40.0%
GATTGAAGGCTTATTGCTGT+TGG - chr3_1:20601519-20601538 Msa0352220:CDS 40.0%
!! CTTGGTGTTGAAACTAAAGG+TGG - chr3_1:20601559-20601578 Msa0352220:CDS 40.0%
GTAGGGATCCTAGTATTCTT+CGG + chr3_1:20601604-20601623 None:intergenic 40.0%
AGGAGAACATGCTAAGACTA+AGG - chr3_1:20601681-20601700 Msa0352220:CDS 40.0%
GATCATTATCACCAACAAGG+AGG - chr3_1:20601849-20601868 Msa0352220:CDS 40.0%
ATGGCTTGACAGAAACCAAT+TGG - chr3_1:20602044-20602063 Msa0352220:CDS 40.0%
TGAATTCAAGAAGCAGGAGT+TGG - chr3_1:20602083-20602102 Msa0352220:CDS 40.0%
AGAAGCAGGAGTTGGAAAAT+AGG - chr3_1:20602091-20602110 Msa0352220:CDS 40.0%
GAAGCAGGAGTTGGAAAATA+GGG - chr3_1:20602092-20602111 Msa0352220:CDS 40.0%
!! AAAGCCATAGGCATTGACCT+TGG - chr3_1:20600338-20600357 Msa0352220:CDS 45.0%
TGTACCAAGGTCAATGCCTA+TGG + chr3_1:20600345-20600364 None:intergenic 45.0%
! TACAGCTGTAGCTTGTACCA+AGG + chr3_1:20600358-20600377 None:intergenic 45.0%
GAGATCATTCCAAACGACCA+AGG - chr3_1:20600404-20600423 Msa0352220:CDS 45.0%
!! GCATCAAAAACGGTGTTGTG+TGG + chr3_1:20600515-20600534 None:intergenic 45.0%
GCGATGACCAATCAAACGTT+TGG + chr3_1:20600537-20600556 None:intergenic 45.0%
TAAGCAATAGCAGCTGCAGT+AGG + chr3_1:20600863-20600882 None:intergenic 45.0%
ACTGCAGCTGCTATTGCTTA+TGG - chr3_1:20600863-20600882 Msa0352220:CDS 45.0%
CTTGTCTTTGATCTTGGTGG+TGG - chr3_1:20600926-20600945 Msa0352220:CDS 45.0%
TCCTTAGTGACTGTTGACGA+AGG - chr3_1:20600962-20600981 Msa0352220:CDS 45.0%
TCCTTCGTCAACAGTCACTA+AGG + chr3_1:20600966-20600985 None:intergenic 45.0%
TGTTGACGAAGGAATGTTCC+AGG - chr3_1:20600973-20600992 Msa0352220:CDS 45.0%
CGAAGGAATGTTCCAGGTTA+AGG - chr3_1:20600979-20600998 Msa0352220:CDS 45.0%
! TTCCAGGTTAAGGCCACTTT+GGG - chr3_1:20600989-20601008 Msa0352220:CDS 45.0%
TTGGGAGATACTCATTTGGG+TGG - chr3_1:20601007-20601026 Msa0352220:CDS 45.0%
!! AGAATTCCAAAGCTTTGGGG+AGG - chr3_1:20601110-20601129 Msa0352220:CDS 45.0%
TCTTAACCTCCCCAAAGCTT+TGG + chr3_1:20601119-20601138 None:intergenic 45.0%
! CAGAAAGTGCATGAAGACTG+TGG - chr3_1:20601276-20601295 Msa0352220:CDS 45.0%
! AATGTTTTCTCCACAGCCCA+AGG - chr3_1:20601634-20601653 Msa0352220:CDS 45.0%
CAAAACTTTACCTTGGGCTG+TGG + chr3_1:20601647-20601666 None:intergenic 45.0%
TTATCACCAACAAGGAGGGA+AGG - chr3_1:20601854-20601873 Msa0352220:CDS 45.0%
ATGAAATGAGGGAGAGAGTG+AGG - chr3_1:20601989-20602008 Msa0352220:CDS 45.0%
GAGAGTGAGGAAGCTTGAAA+AGG - chr3_1:20602002-20602021 Msa0352220:CDS 45.0%
GAGGAAGCTTGAAAAGGTAG+TGG - chr3_1:20602008-20602027 Msa0352220:CDS 45.0%
! GAAGCTTGAAAAGGTAGTGG+AGG - chr3_1:20602011-20602030 Msa0352220:CDS 45.0%
!! AAAGCCATAGGCATTGACCT+TGG - chr3_1:20600338-20600357 Msa0352220:CDS 45.0%
TGTACCAAGGTCAATGCCTA+TGG + chr3_1:20600345-20600364 None:intergenic 45.0%
! TACAGCTGTAGCTTGTACCA+AGG + chr3_1:20600358-20600377 None:intergenic 45.0%
GAGATCATTCCAAACGACCA+AGG - chr3_1:20600404-20600423 Msa0352220:CDS 45.0%
!! GCATCAAAAACGGTGTTGTG+TGG + chr3_1:20600515-20600534 None:intergenic 45.0%
GCGATGACCAATCAAACGTT+TGG + chr3_1:20600537-20600556 None:intergenic 45.0%
TAAGCAATAGCAGCTGCAGT+AGG + chr3_1:20600863-20600882 None:intergenic 45.0%
ACTGCAGCTGCTATTGCTTA+TGG - chr3_1:20600863-20600882 Msa0352220:CDS 45.0%
CTTGTCTTTGATCTTGGTGG+TGG - chr3_1:20600926-20600945 Msa0352220:CDS 45.0%
TCCTTAGTGACTGTTGACGA+AGG - chr3_1:20600962-20600981 Msa0352220:CDS 45.0%
TCCTTCGTCAACAGTCACTA+AGG + chr3_1:20600966-20600985 None:intergenic 45.0%
TGTTGACGAAGGAATGTTCC+AGG - chr3_1:20600973-20600992 Msa0352220:CDS 45.0%
CGAAGGAATGTTCCAGGTTA+AGG - chr3_1:20600979-20600998 Msa0352220:CDS 45.0%
! TTCCAGGTTAAGGCCACTTT+GGG - chr3_1:20600989-20601008 Msa0352220:CDS 45.0%
TTGGGAGATACTCATTTGGG+TGG - chr3_1:20601007-20601026 Msa0352220:CDS 45.0%
!! AGAATTCCAAAGCTTTGGGG+AGG - chr3_1:20601110-20601129 Msa0352220:CDS 45.0%
TCTTAACCTCCCCAAAGCTT+TGG + chr3_1:20601119-20601138 None:intergenic 45.0%
! CAGAAAGTGCATGAAGACTG+TGG - chr3_1:20601276-20601295 Msa0352220:CDS 45.0%
! AATGTTTTCTCCACAGCCCA+AGG - chr3_1:20601634-20601653 Msa0352220:CDS 45.0%
CAAAACTTTACCTTGGGCTG+TGG + chr3_1:20601647-20601666 None:intergenic 45.0%
TTATCACCAACAAGGAGGGA+AGG - chr3_1:20601854-20601873 Msa0352220:CDS 45.0%
ATGAAATGAGGGAGAGAGTG+AGG - chr3_1:20601989-20602008 Msa0352220:CDS 45.0%
GAGAGTGAGGAAGCTTGAAA+AGG - chr3_1:20602002-20602021 Msa0352220:CDS 45.0%
GAGGAAGCTTGAAAAGGTAG+TGG - chr3_1:20602008-20602027 Msa0352220:CDS 45.0%
! GAAGCTTGAAAAGGTAGTGG+AGG - chr3_1:20602011-20602030 Msa0352220:CDS 45.0%
GCTACAGCTGTATTGCAGTG+TGG - chr3_1:20600366-20600385 Msa0352220:CDS 50.0%
CAACGTAAGAAGGGGTGACA+CGG + chr3_1:20600433-20600452 None:intergenic 50.0%
GGTGAAGGCAACGTAAGAAG+GGG + chr3_1:20600441-20600460 None:intergenic 50.0%
CGGTGAAGGCAACGTAAGAA+GGG + chr3_1:20600442-20600461 None:intergenic 50.0%
TCGGTGAAGGCAACGTAAGA+AGG + chr3_1:20600443-20600462 None:intergenic 50.0%
GTTCCAGGTTAAGGCCACTT+TGG - chr3_1:20600988-20601007 Msa0352220:CDS 50.0%
CAGCATGCGAGAAAGCAAAG+AGG - chr3_1:20601140-20601159 Msa0352220:CDS 50.0%
CCATAAGCCACAGCTTCATC+AGG + chr3_1:20601454-20601473 None:intergenic 50.0%
CCTGATGAAGCTGTGGCTTA+TGG - chr3_1:20601451-20601470 Msa0352220:CDS 50.0%
ACTCAACCTTCCCTCCTTGT+TGG + chr3_1:20601863-20601882 None:intergenic 50.0%
ATCCTCTGCCTTATACCGCT+CGG + chr3_1:20601917-20601936 None:intergenic 50.0%
GTATAAGGCAGAGGATGAGG+AGG - chr3_1:20601921-20601940 Msa0352220:CDS 50.0%
GGATGAGGAGGTTAGGAAGA+AGG - chr3_1:20601933-20601952 Msa0352220:CDS 50.0%
GCTACAGCTGTATTGCAGTG+TGG - chr3_1:20600366-20600385 Msa0352220:CDS 50.0%
CAACGTAAGAAGGGGTGACA+CGG + chr3_1:20600433-20600452 None:intergenic 50.0%
GGTGAAGGCAACGTAAGAAG+GGG + chr3_1:20600441-20600460 None:intergenic 50.0%
CGGTGAAGGCAACGTAAGAA+GGG + chr3_1:20600442-20600461 None:intergenic 50.0%
TCGGTGAAGGCAACGTAAGA+AGG + chr3_1:20600443-20600462 None:intergenic 50.0%
GTTCCAGGTTAAGGCCACTT+TGG - chr3_1:20600988-20601007 Msa0352220:CDS 50.0%
CAGCATGCGAGAAAGCAAAG+AGG - chr3_1:20601140-20601159 Msa0352220:CDS 50.0%
CCATAAGCCACAGCTTCATC+AGG + chr3_1:20601454-20601473 None:intergenic 50.0%
CCTGATGAAGCTGTGGCTTA+TGG - chr3_1:20601451-20601470 Msa0352220:CDS 50.0%
ACTCAACCTTCCCTCCTTGT+TGG + chr3_1:20601863-20601882 None:intergenic 50.0%
ATCCTCTGCCTTATACCGCT+CGG + chr3_1:20601917-20601936 None:intergenic 50.0%
GTATAAGGCAGAGGATGAGG+AGG - chr3_1:20601921-20601940 Msa0352220:CDS 50.0%
GGATGAGGAGGTTAGGAAGA+AGG - chr3_1:20601933-20601952 Msa0352220:CDS 50.0%
ACACGGTTCCCTTGGTCGTT+TGG + chr3_1:20600416-20600435 None:intergenic 55.0%
! CTCCCAAAGTGGCCTTAACC+TGG + chr3_1:20600994-20601013 None:intergenic 55.0%
GAATGATGAGGGATGCCGAG+CGG - chr3_1:20601899-20601918 Msa0352220:CDS 55.0%
GCGGTATAAGGCAGAGGATG+AGG - chr3_1:20601918-20601937 Msa0352220:CDS 55.0%
AGGCAGAGGATGAGGAGGTT+AGG - chr3_1:20601926-20601945 Msa0352220:CDS 55.0%
ACACGGTTCCCTTGGTCGTT+TGG + chr3_1:20600416-20600435 None:intergenic 55.0%
! CTCCCAAAGTGGCCTTAACC+TGG + chr3_1:20600994-20601013 None:intergenic 55.0%
GAATGATGAGGGATGCCGAG+CGG - chr3_1:20601899-20601918 Msa0352220:CDS 55.0%
GCGGTATAAGGCAGAGGATG+AGG - chr3_1:20601918-20601937 Msa0352220:CDS 55.0%
AGGCAGAGGATGAGGAGGTT+AGG - chr3_1:20601926-20601945 Msa0352220:CDS 55.0%
AAGGGGTGACACGGTTCCCT+TGG + chr3_1:20600424-20600443 None:intergenic 60.0%
GAGGGATGCCGAGCGGTATA+AGG - chr3_1:20601906-20601925 Msa0352220:CDS 60.0%
TGCCGAGCGGTATAAGGCAG+AGG - chr3_1:20601912-20601931 Msa0352220:CDS 60.0%
AAGGGGTGACACGGTTCCCT+TGG + chr3_1:20600424-20600443 None:intergenic 60.0%
GAGGGATGCCGAGCGGTATA+AGG - chr3_1:20601906-20601925 Msa0352220:CDS 60.0%
TGCCGAGCGGTATAAGGCAG+AGG - chr3_1:20601912-20601931 Msa0352220:CDS 60.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 20600320 20602128 20600320 ID=Msa0352220;Name=Msa0352220
chr3_1 mRNA 20600320 20602128 20600320 ID=Msa0352220-mRNA-1;Parent=Msa0352220;Name=Msa0352220-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|602
chr3_1 exon 20600320 20602128 20600320 ID=Msa0352220-mRNA-1:exon:4723;Parent=Msa0352220-mRNA-1
chr3_1 CDS 20600320 20602128 20600320 ID=Msa0352220-mRNA-1:cds;Parent=Msa0352220-mRNA-1
Gene Sequence

>Msa0352220

ATGGCAACAAGAAAAAGCAAAGCCATAGGCATTGACCTTGGTACAAGCTACAGCTGTATTGCAGTGTGGCGAAACAATTGTGTTGAGATCATTCCAAACGACCAAGGGAACCGTGTCACCCCTTCTTACGTTGCCTTCACCGATACTGAAAGATTAATAGGTGATGCTGCCATAAACCAATCAGCCAAAAATCCACACAACACCGTTTTTGATGCCAAACGTTTGATTGGTCATCGCTTCTCTGACCAATCAGTCCAACAAGACATAAGGTTTTGGCCTTTTAAGGTTGTTCCAAACCACAAAGACAAACCCATGATTGTTGTCAATTACAAAGGCCAAGAAAAACATTTTTCACCTCAAGAGATATCTTCCATGGTGTTGTCTAAATTAAAGGAAATTGCTGAAACTTATTTGGGTCATGATCATGAAGTGAACAAAGCTGTTATCACTGTTCCTGCTTACTTCAACAACTCACAGAGACAAGCTACAAAAGATGCAGGAAAAATTGCCGGTTTTAATGTGATGAGGATCATCAACGAGCCTACTGCAGCTGCTATTGCTTATGGTTTGGACAAGAAAATGTGGAGAGAAGGCGAGAAGAATGTTCTTGTCTTTGATCTTGGTGGTGGAACTTTTGATGTTTCCTTAGTGACTGTTGACGAAGGAATGTTCCAGGTTAAGGCCACTTTGGGAGATACTCATTTGGGTGGTGTTGATTTTGATAACAATTTGGTGAACCATCTTGTTGAATTATTTCATAGAAAGTACAAGAAGGATTTGAAGATCAGTGAGAATTCCAAAGCTTTGGGGAGGTTAAGATCAGCATGCGAGAAAGCAAAGAGGTTACTTTCTTCATCATCACAAACAGTTGTTGAGCTTGATTCACTATGTGAAGGGATTGATCTACATTTCACTGTTACTAGAGCTTTTTTTGAGGAAATAAACAAGGATTTGTTCAGAAAGTGCATGAAGACTGTGGAGAAGTGTTTGATTGAGGCAAAAATTAGTAAAAGCCAAGTTCATGAATTTGTTATTGTCGGAGGGTCTACTAGAATTCCGAAGATTCAACAACTACTGAAGGAAATGTTTAGTGTCAACGGCGAAGTCAAAGAGCCTTGCAAAAGCATTAATCCTGATGAAGCTGTGGCTTATGGCGCAGCAGTTCAAGCAGCAATATTGAATACTGAAGGAGACAAGAAGATTGAAGGCTTATTGCTGTTGGATGTTATGCCTTTTAGTCTTGGTGTTGAAACTAAAGGTGGTGTCATGTCTGTTTTGATTCCGAAGAATACTAGGATCCCTACAAAAAAGGAGAATGTTTTCTCCACAGCCCAAGGTAAAGTTTTGATCAAAGTCTATGAAGGAGAACATGCTAAGACTAAGGACAACTTTTTGCTTGGTAAATTTGAGCTCTCAGAATGCTCTTTAGTACCTAGAAAGGTTCCAAATATCAATATTTGTTTTGATGTTGATGTTAATGGAATTTTGGAGGTTACTGCTGAAGATAAGACAAAAGGGTTGAAACAGAAGATCATTATCACCAACAAGGAGGGAAGGTTGAGTCCTCAAGAGATGAGAAGAATGATGAGGGATGCCGAGCGGTATAAGGCAGAGGATGAGGAGGTTAGGAAGAAGGTGAAAGCAAAGAATTTGTTTGAGAATTATGTTTATGAAATGAGGGAGAGAGTGAGGAAGCTTGAAAAGGTAGTGGAGGAAAGTATAGAATGGCTTGACAGAAACCAATTGGCAGAAATTGATGAGTTTGAATTCAAGAAGCAGGAGTTGGAAAATAGGGTGAAGTTTCTTTAA
Protein sequence

>Msa0352220

MATRKSKAIGIDLGTSYSCIAVWRNNCVEIIPNDQGNRVTPSYVAFTDTERLIGDAAINQSAKNPHNTVFDAKRLIGHRFSDQSVQQDIRFWPFKVVPNHKDKPMIVVNYKGQEKHFSPQEISSMVLSKLKEIAETYLGHDHEVNKAVITVPAYFNNSQRQATKDAGKIAGFNVMRIINEPTAAAIAYGLDKKMWREGEKNVLVFDLGGGTFDVSLVTVDEGMFQVKATLGDTHLGGVDFDNNLVNHLVELFHRKYKKDLKISENSKALGRLRSACEKAKRLLSSSSQTVVELDSLCEGIDLHFTVTRAFFEEINKDLFRKCMKTVEKCLIEAKISKSQVHEFVIVGGSTRIPKIQQLLKEMFSVNGEVKEPCKSINPDEAVAYGAAVQAAILNTEGDKKIEGLLLLDVMPFSLGVETKGGVMSVLIPKNTRIPTKKENVFSTAQGKVLIKVYEGEHAKTKDNFLLGKFELSECSLVPRKVPNINICFDVDVNGILEVTAEDKTKGLKQKIIITNKEGRLSPQEMRRMMRDAERYKAEDEEVRKKVKAKNLFENYVYEMRERVRKLEKVVEESIEWLDRNQLAEIDEFEFKKQELENRVKFL*