AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0493850

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0493850 MtrunA17_Chr2g0284681 92.231 605 42 2 1 602 1 603 0.0 1143
Msa0493850 MtrunA17_Chr4g0073741 68.352 613 169 6 1 596 1 605 0.0 857
Msa0493850 MtrunA17_Chr4g0031301 66.997 606 175 6 8 596 9 606 0.0 819
Msa0493850 MtrunA17_Chr4g0031321 66.667 606 177 6 8 596 9 606 0.0 814
Msa0493850 MtrunA17_Chr7g0223181 66.227 607 180 6 8 597 9 607 0.0 810
Msa0493850 MtrunA17_Chr7g0262821 65.842 606 182 6 8 596 9 606 0.0 806
Msa0493850 MtrunA17_Chr7g0223291 66.172 606 180 6 8 596 9 606 0.0 805
Msa0493850 MtrunA17_Chr6g0462681 55.333 600 242 6 8 590 9 599 0.0 668
Msa0493850 MtrunA17_Chr6g0462601 56.000 600 238 6 8 590 9 599 0.0 655
Msa0493850 MtrunA17_Chr8g0388671 55.426 599 236 8 2 581 32 618 0.0 650
Msa0493850 MtrunA17_Chr7g0223741 58.363 562 219 7 5 561 6 557 0.0 650
Msa0493850 MtrunA17_Chr8g0388761 54.682 598 240 8 3 581 33 618 0.0 647
Msa0493850 MtrunA17_Chr5g0427901 53.920 625 248 9 8 602 9 623 0.0 644
Msa0493850 MtrunA17_Chr7g0223751 56.940 562 226 9 8 564 9 559 0.0 639
Msa0493850 MtrunA17_Chr7g0223711 56.762 562 228 7 5 561 6 557 0.0 636
Msa0493850 MtrunA17_Chr7g0221661 54.844 609 246 9 8 597 9 607 0.0 636
Msa0493850 MtrunA17_Chr2g0298851 54.045 618 254 10 5 602 6 613 0.0 632
Msa0493850 MtrunA17_Chr8g0350731 57.245 559 223 8 8 561 37 584 0.0 625
Msa0493850 MtrunA17_Chr2g0293581 53.566 603 251 9 7 589 9 602 0.0 625
Msa0493850 MtrunA17_Chr7g0233311 57.549 563 225 6 8 565 9 562 0.0 625
Msa0493850 MtrunA17_Chr7g0223731 56.406 562 229 8 5 561 6 556 0.0 624
Msa0493850 MtrunA17_Chr6g0462771 52.231 605 262 7 5 589 6 603 0.0 620
Msa0493850 MtrunA17_Chr7g0223721 53.008 615 259 10 8 602 9 613 0.0 619
Msa0493850 MtrunA17_Chr6g0462711 52.815 604 261 7 5 589 7 605 0.0 617
Msa0493850 MtrunA17_Chr7g0223801 52.217 609 260 10 8 597 9 605 0.0 616
Msa0493850 MtrunA17_Chr6g0462641 52.632 608 264 7 1 589 1 603 0.0 616
Msa0493850 MtrunA17_Chr7g0223701 53.247 616 253 9 8 597 9 615 0.0 615
Msa0493850 MtrunA17_Chr7g0223781 55.654 566 234 8 8 568 9 562 0.0 615
Msa0493850 MtrunA17_Chr7g0225671 53.537 622 258 11 1 602 9 619 0.0 615
Msa0493850 MtrunA17_Chr7g0223621 55.160 562 236 8 8 564 9 559 0.0 612
Msa0493850 MtrunA17_Chr7g0223031 54.982 562 237 8 5 561 6 556 0.0 612
Msa0493850 MtrunA17_Chr2g0287631 52.520 615 263 9 8 602 9 614 0.0 607
Msa0493850 MtrunA17_Chr7g0223851 52.666 619 261 11 5 602 15 622 0.0 605
Msa0493850 MtrunA17_Chr7g0248131 55.923 574 227 12 8 573 9 564 0.0 604
Msa0493850 MtrunA17_Chr2g0298861 51.597 626 261 12 9 602 10 625 0.0 602
Msa0493850 MtrunA17_Chr4g0040931 52.033 615 265 10 8 602 9 613 0.0 602
Msa0493850 MtrunA17_Chr7g0223631 54.383 559 225 10 8 561 9 542 0.0 588
Msa0493850 MtrunA17_Chr7g0248151 55.893 560 226 9 8 559 9 555 0.0 588
Msa0493850 MtrunA17_Chr2g0296391 56.015 532 220 6 4 530 5 527 0.0 582
Msa0493850 MtrunA17_Chr7g0233301 54.626 562 216 6 8 567 9 533 0.0 582
Msa0493850 MtrunA17_Chr7g0217971 51.248 601 239 10 8 589 9 574 0.0 575
Msa0493850 MtrunA17_Chr7g0223821 51.286 622 268 10 5 602 6 616 0.0 575
Msa0493850 MtrunA17_Chr7g0215261 52.920 565 215 8 7 565 8 527 0.0 557
Msa0493850 MtrunA17_Chr6g0462611 52.459 549 233 7 59 590 10 547 0.0 555
Msa0493850 MtrunA17_Chr4g0012541 49.435 619 272 12 5 602 6 604 0.0 552
Msa0493850 MtrunA17_Chr8g0390821 47.121 660 254 13 8 597 11 645 0.0 550
Msa0493850 MtrunA17_Chr7g0223831 52.473 566 226 9 5 565 6 533 0.0 547
Msa0493850 MtrunA17_Chr2g0331381 52.407 540 186 8 9 546 7 477 0.0 545
Msa0493850 MtrunA17_Chr7g0223541 54.125 497 215 6 71 564 31 517 0.0 525
Msa0493850 MtrunA17_Chr7g0228281 47.890 616 246 11 7 602 69 629 1.38e-180 525
Msa0493850 MtrunA17_Chr2g0293601 53.295 516 199 7 8 521 9 484 2.35e-178 513
Msa0493850 MtrunA17_Chr7g0223681 54.298 477 204 7 88 561 2 467 3.33e-175 507
Msa0493850 MtrunA17_Chr2g0299921 66.581 389 120 4 8 396 1029 1407 7.66e-168 522
Msa0493850 MtrunA17_Chr2g0299921 65.979 388 121 6 8 394 1413 1790 1.36e-154 486
Msa0493850 MtrunA17_Chr2g0299821 60.880 409 145 6 9 411 10 409 2.67e-166 483
Msa0493850 MtrunA17_Chr2g0299821 41.429 140 59 3 425 541 343 482 1.03e-21 98.6
Msa0493850 MtrunA17_Chr2g0293561 62.567 374 130 4 8 379 9 374 4.13e-165 476
Msa0493850 MtrunA17_Chr2g0299851 63.824 387 130 5 8 394 1038 1414 3.42e-162 500
Msa0493850 MtrunA17_Chr2g0279901 46.037 593 275 16 9 583 57 622 2.88e-161 477
Msa0493850 MtrunA17_Chr4g0049501 44.610 603 294 14 6 591 51 630 2.10e-157 467
Msa0493850 MtrunA17_Chr2g0299791 63.824 387 130 5 8 394 1042 1418 1.26e-156 485
Msa0493850 MtrunA17_Chr6g0462861 46.410 571 260 14 8 563 9 548 8.22e-155 459
Msa0493850 MtrunA17_Chr1g0166551 43.092 608 209 11 8 597 5 493 1.09e-149 441
Msa0493850 MtrunA17_Chr2g0299881 60.870 391 139 6 8 394 1038 1418 3.05e-147 461
Msa0493850 MtrunA17_Chr2g0299881 62.121 66 21 1 184 249 1414 1475 3.67e-16 82.8
Msa0493850 MtrunA17_Chr2g0277021 44.923 581 286 14 7 575 70 628 1.42e-145 437
Msa0493850 MtrunA17_Chr2g0293271 47.865 445 148 6 20 459 43 408 3.73e-131 390
Msa0493850 MtrunA17_Chr1g0151991 62.069 290 104 3 5 292 6 291 8.88e-125 369
Msa0493850 MtrunA17_Chr7g0245521 65.056 269 88 3 8 274 9 273 3.51e-119 354
Msa0493850 MtrunA17_Chr6g0462631 48.529 340 124 5 83 422 9 297 1.54e-96 297
Msa0493850 MtrunA17_Chr6g0462751 42.936 361 139 5 61 419 9 304 1.88e-90 282
Msa0493850 MtrunA17_Chr7g0223761 39.142 373 166 5 240 597 3 329 1.41e-80 257
Msa0493850 MtrunA17_Chr7g0223671 59.896 192 75 1 71 262 8 197 3.10e-78 246
Msa0493850 MtrunA17_Chr1g0188601 62.176 193 69 2 91 283 3 191 5.58e-77 243
Msa0493850 MtrunA17_Chr4g0004331 34.081 446 270 6 9 441 4 438 5.03e-76 258
Msa0493850 MtrunA17_Chr5g0394391 31.953 507 315 12 8 498 29 521 4.71e-75 250
Msa0493850 MtrunA17_Chr2g0331901 32.075 477 298 8 9 470 4 469 8.07e-75 255
Msa0493850 MtrunA17_Chr7g0245531 44.013 309 150 6 306 597 2 304 1.50e-73 238
Msa0493850 MtrunA17_Chr6g0462791 60.556 180 66 3 105 284 1 175 3.20e-68 219
Msa0493850 MtrunA17_Chr5g0444001 31.333 450 289 7 9 452 4 439 2.11e-67 229
Msa0493850 MtrunA17_Chr3g0098761 29.365 504 334 8 9 502 4 495 2.53e-66 231
Msa0493850 MtrunA17_Chr1g0152001 40.924 303 155 7 317 602 2 297 1.68e-65 217
Msa0493850 MtrunA17_Chr7g0223771 64.935 154 52 1 91 244 2 153 9.69e-65 209
Msa0493850 MtrunA17_Chr4g0060241 30.512 508 315 12 9 500 21 506 4.19e-58 204
Msa0493850 MtrunA17_Chr4g0064621 32.068 527 325 13 8 512 140 655 4.58e-58 206
Msa0493850 MtrunA17_Chr1g0188611 48.133 241 116 5 323 560 1 235 1.57e-54 187
Msa0493850 MtrunA17_Chr7g0223861 65.414 133 44 1 75 207 4 134 2.65e-54 181
Msa0493850 MtrunA17_Chr4g0046881 61.074 149 51 3 128 276 10 151 1.63e-51 174
Msa0493850 MtrunA17_Chr3g0120101 26.554 531 346 11 6 502 24 544 3.05e-51 189
Msa0493850 MtrunA17_Chr6g0462701 52.353 170 53 3 102 271 27 168 2.69e-46 160
Msa0493850 MtrunA17_Chr5g0394231 40.693 231 82 5 71 300 5 181 1.91e-45 159
Msa0493850 MtrunA17_Chr6g0462691 52.740 146 62 2 323 465 1 142 1.73e-44 155
Msa0493850 MtrunA17_Chr5g0442171 33.138 341 188 11 169 491 1 319 2.95e-44 160
Msa0493850 MtrunA17_Chr5g0394221 36.431 269 157 6 300 563 69 328 1.28e-42 157
Msa0493850 MtrunA17_Chr6g0481391 53.226 124 53 2 35 158 13 131 1.08e-34 127
Msa0493850 MtrunA17_Chr2g0284661 62.766 94 32 1 362 452 50 143 1.10e-29 114
Msa0493850 MtrunA17_Chr2g0284661 83.636 55 9 0 224 278 5 59 9.08e-24 97.4
Msa0493850 MtrunA17_Chr4g0046871 54.717 106 43 2 336 441 5 105 1.26e-29 112
Msa0493850 MtrunA17_Chr6g0462621 70.423 71 20 1 1 70 1 71 4.10e-28 107
Msa0493850 MtrunA17_Chr7g0245541 34.896 192 107 3 423 597 1 191 1.52e-27 110
Msa0493850 MtrunA17_Chr6g0481381 63.514 74 27 0 175 248 1 74 2.34e-25 103
Msa0493850 MtrunA17_Chr7g0223691 70.769 65 17 1 8 70 9 73 6.14e-25 98.6
Msa0493850 MtrunA17_Chr1g0188591 64.706 68 22 1 5 70 6 73 9.50e-24 95.5
Msa0493850 MtrunA17_Chr7g0223791 52.439 82 31 2 53 134 18 91 2.28e-19 86.3
Msa0493850 MtrunA17_Chr7g0223791 37.975 79 49 0 486 564 90 168 3.50e-12 65.5
Msa0493850 MtrunA17_Chr7g0223881 50.667 75 36 1 289 363 9 82 9.64e-18 78.6
Msa0493850 MtrunA17_Chr6g0462801 53.030 66 30 1 323 387 1 66 3.46e-16 73.6
Msa0493850 MtrunA17_Chr7g0221651 40.698 86 49 1 214 299 1 84 5.76e-15 70.5
Msa0493850 MtrunA17_Chr4g0064651 38.462 91 53 1 404 491 30 120 8.89e-15 71.2
Msa0493850 MtrunA17_Chr2g0299891 69.048 42 13 0 317 358 2 43 5.50e-14 67.4
Msa0493850 MtrunA17_Chr5g0442161 30.065 153 100 4 8 156 28 177 6.44e-13 67.4
Msa0493850 MtrunA17_Chr2g0299941 60.000 55 19 1 405 456 23 77 7.33e-12 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0493850 AT1G16030.1 67.047 613 177 6 1 596 1 605 0.0 832
Msa0493850 AT3G12580.1 67.051 607 175 6 8 597 9 607 0.0 815
Msa0493850 AT5G02500.1 66.227 607 180 6 8 597 9 607 0.0 808
Msa0493850 AT3G09440.1 66.063 607 181 6 8 597 9 607 0.0 801
Msa0493850 AT3G09440.4 66.063 607 181 6 8 597 9 607 0.0 801
Msa0493850 AT3G09440.2 66.063 607 181 6 8 597 9 607 0.0 801
Msa0493850 AT3G09440.3 66.063 607 181 6 8 597 9 607 0.0 801
Msa0493850 AT5G02490.1 65.404 607 185 6 8 597 9 607 0.0 795
Msa0493850 AT1G56410.1 65.124 605 188 7 8 597 9 605 0.0 787
Msa0493850 AT5G42020.1 55.700 614 241 8 2 596 31 632 0.0 670
Msa0493850 AT5G42020.3 55.700 614 241 8 2 596 36 637 0.0 670
Msa0493850 AT5G28540.1 55.537 614 242 8 2 596 31 632 0.0 667
Msa0493850 AT1G09080.1 55.611 606 239 7 9 596 53 646 0.0 649
Msa0493850 AT1G09080.2 55.611 606 239 7 9 596 43 636 0.0 649
Msa0493850 AT5G42020.2 51.833 600 231 11 2 596 31 577 0.0 583
Msa0493850 AT5G02500.2 70.558 394 108 4 8 401 9 394 0.0 570
Msa0493850 AT5G02500.2 45.652 92 36 1 520 597 386 477 8.58e-16 80.9
Msa0493850 AT5G09590.1 45.983 585 277 15 9 577 60 621 2.07e-156 465
Msa0493850 AT4G37910.1 46.316 570 268 13 9 563 55 601 1.79e-151 452
Msa0493850 AT4G37910.2 46.316 570 268 13 9 563 55 601 1.79e-151 452
Msa0493850 AT5G49910.1 44.234 581 290 14 7 575 79 637 4.30e-144 434
Msa0493850 AT4G24280.1 44.578 581 288 15 7 575 79 637 5.69e-144 434
Msa0493850 AT1G79930.2 34.305 446 269 6 9 441 4 438 1.48e-79 267
Msa0493850 AT1G79930.1 34.305 446 269 6 9 441 4 438 4.19e-79 267
Msa0493850 AT1G79920.1 34.305 446 269 6 9 441 4 438 1.06e-78 264
Msa0493850 AT1G79920.3 34.305 446 269 6 9 441 4 438 1.37e-78 263
Msa0493850 AT1G79920.2 34.305 446 269 6 9 441 4 438 1.37e-78 263
Msa0493850 AT1G79920.4 34.305 446 269 6 9 441 4 438 1.37e-78 263
Msa0493850 AT1G11660.4 28.418 651 391 16 9 602 4 636 2.18e-73 250
Msa0493850 AT1G11660.3 28.418 651 391 16 9 602 4 636 2.18e-73 250
Msa0493850 AT1G11660.1 28.418 651 391 16 9 602 4 636 2.18e-73 250
Msa0493850 AT2G32120.1 30.077 522 322 13 8 512 30 525 2.52e-65 224
Msa0493850 AT2G32120.2 30.077 522 322 13 8 512 30 525 2.52e-65 224
Msa0493850 AT1G11660.2 28.247 616 367 16 44 602 2 599 2.47e-64 225
Msa0493850 AT4G16660.2 26.154 455 303 8 5 436 47 491 6.83e-48 180
Msa0493850 AT4G16660.1 26.154 455 303 8 5 436 23 467 7.93e-48 180

Find 120 sgRNAs with CRISPR-Local

Find 288 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAAATTGCTGAAACTTATT+TGG 0.082085 3_4:-20152026 Msa0493850:CDS
CATGGTGTTGTCTAAATTAA+AGG 0.157364 3_4:-20152047 Msa0493850:CDS
TCAAAACAAATATTGATATT+TGG 0.181030 3_4:+20150975 None:intergenic
TTGATATTTGGAACCTTTCT+AGG 0.182799 3_4:+20150987 None:intergenic
ACTGCAGCTGCTATTGCTTA+TGG 0.208608 3_4:-20151875 Msa0493850:CDS
TGGTTTGGAACAACCTTAAA+AGG 0.212043 3_4:+20152142 None:intergenic
TGTTGATGTTAATGGAATTT+TGG 0.222928 3_4:-20150952 Msa0493850:CDS
GTTTCAACACCAAGACTAAA+AGG 0.273894 3_4:+20151188 None:intergenic
CAATCATGGGTTTGTCTTTG+TGG 0.290878 3_4:+20152122 None:intergenic
CAGTGAGAATTCCAAAGCTT+TGG 0.318063 3_4:-20151633 Msa0493850:CDS
CCTGATGAAGCTGTGGCTTA+TGG 0.330474 3_4:-20151287 Msa0493850:CDS
AATGTTCTTGTCTTTGATCT+TGG 0.331932 3_4:-20151818 Msa0493850:CDS
AGTGAGAATTCCAAAGCTTT+GGG 0.334628 3_4:-20151632 Msa0493850:CDS
GAAATTGCTGAAACTTATTT+GGG 0.342870 3_4:-20152025 Msa0493850:CDS
ACACGGTTCCCTTGGTCGTT+TGG 0.354947 3_4:+20152325 None:intergenic
CGAAGGAATGTTCCAGGTTA+AGG 0.357597 3_4:-20151759 Msa0493850:CDS
GAGGGATGCCGAGCGGTATA+AGG 0.359508 3_4:-20150832 Msa0493850:CDS
ACTTTGGGAGATACTCATTT+GGG 0.360641 3_4:-20151734 Msa0493850:CDS
TTCCAGGTTAAGGCCACTTT+GGG 0.364020 3_4:-20151749 Msa0493850:CDS
TTATGTCTTGTTGGACTGAT+TGG 0.374106 3_4:+20152173 None:intergenic
AGCTGCTATTGCTTATGGTT+TGG 0.376546 3_4:-20151870 Msa0493850:CDS
CACTTTGGGAGATACTCATT+TGG 0.379211 3_4:-20151735 Msa0493850:CDS
ATGGGTTTGTCTTTGTGGTT+TGG 0.379293 3_4:+20152127 None:intergenic
ACTCATCAATTTCTGCCAAT+TGG 0.383486 3_4:+20150679 None:intergenic
GTCAGTAGTTGTTGAATCTT+CGG 0.384350 3_4:+20151362 None:intergenic
TCCAACAAGACATAAGGTTT+TGG 0.384795 3_4:-20152165 Msa0493850:CDS
ACCGACACTGAAAGATTAAT+AGG 0.390230 3_4:-20152280 Msa0493850:CDS
AGGCAGAGGATGAGGAGGTT+AGG 0.400721 3_4:-20150812 Msa0493850:CDS
TCTTAACCTCCCCAAAGCTT+TGG 0.400779 3_4:+20151622 None:intergenic
ACTCAACCTTCCCTCCTTGT+TGG 0.402188 3_4:+20150878 None:intergenic
CAATAACAAATTCATGAACT+TGG 0.402514 3_4:+20151405 None:intergenic
CTTGTCTTTGATCTTGGTGG+TGG 0.402810 3_4:-20151812 Msa0493850:CDS
GCCAAAACCTTATGTCTTGT+TGG 0.404961 3_4:+20152164 None:intergenic
TTTGATGCCAAACGTTTGAT+TGG 0.412646 3_4:-20152211 Msa0493850:CDS
GTTCTTGTCTTTGATCTTGG+TGG 0.414303 3_4:-20151815 Msa0493850:CDS
GTTCATGAATTTGTTATTGT+CGG 0.430114 3_4:-20151401 Msa0493850:CDS
AGAATTATGTTTATGAAATG+AGG 0.432257 3_4:-20150761 Msa0493850:CDS
GTAGGGATCCTAGTATTCTT+CGG 0.433514 3_4:+20151137 None:intergenic
ATGAAATGAGGGAGAGAGTG+AGG 0.434187 3_4:-20150749 Msa0493850:CDS
TTTGTAATTGACAACAATCA+TGG 0.440479 3_4:+20152108 None:intergenic
CTCCCAAAGTGGCCTTAACC+TGG 0.442345 3_4:+20151747 None:intergenic
TGTTGACGAAGGAATGTTCC+AGG 0.444778 3_4:-20151765 Msa0493850:CDS
GTTCCAGGTTAAGGCCACTT+TGG 0.448544 3_4:-20151750 Msa0493850:CDS
TCGGTGAAGGCAACGTAAGA+AGG 0.448873 3_4:+20152298 None:intergenic
GGATGAGGAGGTTAGGAAGA+AGG 0.449706 3_4:-20150805 Msa0493850:CDS
GATTGAAGGCTTATTGCTGT+TGG 0.451601 3_4:-20151219 Msa0493850:CDS
ATGCTCTTTAGTACCTAGAA+AGG 0.452071 3_4:-20151000 Msa0493850:CDS
TTTGATCAAAACATTACCTT+GGG 0.452396 3_4:+20151088 None:intergenic
CCATAAGCCACAGCTTCATC+AGG 0.453102 3_4:+20151287 None:intergenic
ATCATTCTTCTCATCTCTTG+AGG 0.461303 3_4:+20150855 None:intergenic
AGTCTTGGTGTTGAAACTAA+AGG 0.463181 3_4:-20151182 Msa0493850:CDS
TATGAAATAATTCAACAAGA+TGG 0.465475 3_4:+20151681 None:intergenic
ACTGCTGAAGATAAGACAAA+AGG 0.472237 3_4:-20150924 Msa0493850:CDS
GCGGTATAAGGCAGAGGATG+AGG 0.474792 3_4:-20150820 Msa0493850:CDS
CTTTGATCAAAACATTACCT+TGG 0.475667 3_4:+20151087 None:intergenic
GCGATGACCAATCAAACGTT+TGG 0.484985 3_4:+20152204 None:intergenic
AGGAGAACATGCTAAGACTA+AGG 0.487241 3_4:-20151057 Msa0493850:CDS
GAGGAAGCTTGAAAAGGTAG+TGG 0.489230 3_4:-20150730 Msa0493850:CDS
GAAGGAGACAAGAAGATTGA+AGG 0.489825 3_4:-20151233 Msa0493850:CDS
TGAGTTTGAATTCAAGAAGC+AGG 0.491801 3_4:-20150661 Msa0493850:CDS
TGAATTCAAGAAGCAGGAGT+TGG 0.497584 3_4:-20150655 Msa0493850:CDS
GATCCTCATCACATTAAAAC+CGG 0.502743 3_4:+20151910 None:intergenic
GAGAGTGAGGAAGCTTGAAA+AGG 0.502774 3_4:-20150736 Msa0493850:CDS
GAATTATGTTTATGAAATGA+GGG 0.504803 3_4:-20150760 Msa0493850:CDS
ATGGCTTGACAGAAACCAAT+TGG 0.505887 3_4:-20150694 Msa0493850:CDS
CTGCTGAAGATAAGACAAAA+GGG 0.507869 3_4:-20150923 Msa0493850:CDS
AATCAGTCCAACAAGACATA+AGG 0.516258 3_4:-20152171 Msa0493850:CDS
AAGGGGTGACACGGTTCCCT+TGG 0.517703 3_4:+20152317 None:intergenic
TGTACCAAGGTCAATGCCTA+TGG 0.520221 3_4:+20152396 None:intergenic
ACCTATTAATCTTTCAGTGT+CGG 0.522436 3_4:+20152279 None:intergenic
CATGAATTTGTTATTGTCGG+AGG 0.522698 3_4:-20151398 Msa0493850:CDS
TGTGAGTTGTTGAAGTAAGC+AGG 0.526143 3_4:+20151965 None:intergenic
TTGTAATTGACAACAATCAT+GGG 0.527199 3_4:+20152109 None:intergenic
AGCTTGATTCACTATGTGAA+GGG 0.532350 3_4:-20151544 Msa0493850:CDS
CGGTGAAGGCAACGTAAGAA+GGG 0.538770 3_4:+20152299 None:intergenic
TAGTGGAGGAAAGTATAGAA+TGG 0.539580 3_4:-20150713 Msa0493850:CDS
TCCTTCGTCAACAGTCACTA+AGG 0.546693 3_4:+20151775 None:intergenic
TGCCGAGCGGTATAAGGCAG+AGG 0.550389 3_4:-20150826 Msa0493850:CDS
ACCTCAAGAGATATCTTCCA+TGG 0.553297 3_4:-20152065 Msa0493850:CDS
ATGAATTTGTTATTGTCGGA+GGG 0.553906 3_4:-20151397 Msa0493850:CDS
ATTTCATAGAAAGTACAAGA+AGG 0.557642 3_4:-20151666 Msa0493850:CDS
ATCCTCTGCCTTATACCGCT+CGG 0.563812 3_4:+20150824 None:intergenic
TAATCTTTCAGTGTCGGTGA+AGG 0.565703 3_4:+20152285 None:intergenic
GCAGCAATATTGAATACTGA+AGG 0.571285 3_4:-20151251 Msa0493850:CDS
TTGGGAGATACTCATTTGGG+TGG 0.571754 3_4:-20151731 Msa0493850:CDS
GACAAGAAAATGTGGAGAGA+AGG 0.578701 3_4:-20151848 Msa0493850:CDS
ATGATTGTTGTCAATTACAA+AGG 0.584826 3_4:-20152106 Msa0493850:CDS
AGACAAGCTACAAAAGATGC+AGG 0.588448 3_4:-20151941 Msa0493850:CDS
ATCATTATCACCAACAAGGA+GGG 0.590966 3_4:-20150888 Msa0493850:CDS
AGAGATGAGAAGAATGATGA+GGG 0.595789 3_4:-20150850 Msa0493850:CDS
GAAGCTTGAAAAGGTAGTGG+AGG 0.595927 3_4:-20150727 Msa0493850:CDS
TGTGGAGAAGTGTTTGATTG+AGG 0.604035 3_4:-20151444 Msa0493850:CDS
TACAGCTGTAGCTTGTACCA+AGG 0.607846 3_4:+20152383 None:intergenic
AGAATTCCAAAGCTTTGGGG+AGG 0.611200 3_4:-20151628 Msa0493850:CDS
CATTAATCCTGATGAAGCTG+TGG 0.613030 3_4:-20151294 Msa0493850:CDS
TAAGCAATAGCAGCTGCAGT+AGG 0.618882 3_4:+20151878 None:intergenic
GAAGATTCAACAACTACTGA+CGG 0.621485 3_4:-20151360 Msa0493850:CDS
ACGGAAATGTTTAGTGTCAA+CGG 0.621948 3_4:-20151341 Msa0493850:CDS
GTGAGAATTCCAAAGCTTTG+GGG 0.624343 3_4:-20151631 Msa0493850:CDS
AAGAGATGAGAAGAATGATG+AGG 0.628540 3_4:-20150851 Msa0493850:CDS
CAACGTAAGAAGGGGTGACA+CGG 0.629570 3_4:+20152308 None:intergenic
AGATCATTCCAAACGACCAA+GGG 0.630317 3_4:-20152333 Msa0493850:CDS
GAGATCATTCCAAACGACCA+AGG 0.630381 3_4:-20152334 Msa0493850:CDS
ATGGTTTGGACAAGAAAATG+TGG 0.630603 3_4:-20151856 Msa0493850:CDS
TTATCACCAACAAGGAGGGA+AGG 0.634341 3_4:-20150884 Msa0493850:CDS
GAGCTTGATTCACTATGTGA+AGG 0.634799 3_4:-20151545 Msa0493850:CDS
CAAATGAGTATCTCCCAAAG+TGG 0.637746 3_4:+20151736 None:intergenic
AAAGCCATAGGCATTGACCT+TGG 0.638972 3_4:-20152400 Msa0493850:CDS
TCCTTAGTGACTGTTGACGA+AGG 0.640013 3_4:-20151776 Msa0493850:CDS
ACCATGGAAGATATCTCTTG+AGG 0.641800 3_4:+20152064 None:intergenic
GTATAAGGCAGAGGATGAGG+AGG 0.650304 3_4:-20150817 Msa0493850:CDS
CAGCATGCGAGAAAGCAAAG+AGG 0.658762 3_4:-20151598 Msa0493850:CDS
GGTGAAGGCAACGTAAGAAG+GGG 0.669245 3_4:+20152300 None:intergenic
GATCATTATCACCAACAAGG+AGG 0.670278 3_4:-20150889 Msa0493850:CDS
CTTTAATTTAGACAACACCA+TGG 0.682894 3_4:+20152048 None:intergenic
CAGAAAGTGCATGAAGACTG+TGG 0.701540 3_4:-20151462 Msa0493850:CDS
CTTGGTGTTGAAACTAAAGG+TGG 0.707841 3_4:-20151179 Msa0493850:CDS
GAATGATGAGGGATGCCGAG+CGG 0.719182 3_4:-20150839 Msa0493850:CDS
GAAGATCATTATCACCAACA+AGG 0.723817 3_4:-20150892 Msa0493850:CDS
GCTACAGCTGTATTGCAGTG+TGG 0.732257 3_4:-20152372 Msa0493850:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCAAAACAAATATTGATATT+TGG + chr3_4:20152078-20152097 None:intergenic 15.0%
!! TCAAAACAAATATTGATATT+TGG + chr3_4:20152078-20152097 None:intergenic 15.0%
!!! TGTTGATTTTGATAACAATT+TGG - chr3_4:20151342-20151361 Msa0493850:CDS 20.0%
!! TATGAAATAATTCAACAAGA+TGG + chr3_4:20151372-20151391 None:intergenic 20.0%
!!! TTTTTTTGAGGAAATAAACA+AGG - chr3_4:20151558-20151577 Msa0493850:CDS 20.0%
!! AGAATTATGTTTATGAAATG+AGG - chr3_4:20152289-20152308 Msa0493850:CDS 20.0%
!! GAATTATGTTTATGAAATGA+GGG - chr3_4:20152290-20152309 Msa0493850:CDS 20.0%
!!! TGTTGATTTTGATAACAATT+TGG - chr3_4:20151342-20151361 Msa0493850:CDS 20.0%
!! TATGAAATAATTCAACAAGA+TGG + chr3_4:20151372-20151391 None:intergenic 20.0%
!!! TTTTTTTGAGGAAATAAACA+AGG - chr3_4:20151558-20151577 Msa0493850:CDS 20.0%
!! AGAATTATGTTTATGAAATG+AGG - chr3_4:20152289-20152308 Msa0493850:CDS 20.0%
!! GAATTATGTTTATGAAATGA+GGG - chr3_4:20152290-20152309 Msa0493850:CDS 20.0%
! TTGTAATTGACAACAATCAT+GGG + chr3_4:20150944-20150963 None:intergenic 25.0%
! TTTGTAATTGACAACAATCA+TGG + chr3_4:20150945-20150964 None:intergenic 25.0%
! ATGATTGTTGTCAATTACAA+AGG - chr3_4:20150944-20150963 Msa0493850:CDS 25.0%
! GAAATTGCTGAAACTTATTT+GGG - chr3_4:20151025-20151044 Msa0493850:CDS 25.0%
!! ATTTCATAGAAAGTACAAGA+AGG - chr3_4:20151384-20151403 Msa0493850:CDS 25.0%
! CAATAACAAATTCATGAACT+TGG + chr3_4:20151648-20151667 None:intergenic 25.0%
!!! GTTCATGAATTTGTTATTGT+CGG - chr3_4:20151649-20151668 Msa0493850:CDS 25.0%
!!! AAACATTCTCCTTTTTTGTA+GGG + chr3_4:20151933-20151952 None:intergenic 25.0%
!!! AAAACATTCTCCTTTTTTGT+AGG + chr3_4:20151934-20151953 None:intergenic 25.0%
! TTTGATCAAAACATTACCTT+GGG + chr3_4:20151965-20151984 None:intergenic 25.0%
!!! TGTTTTGATGTTGATGTTAA+TGG - chr3_4:20152090-20152109 Msa0493850:CDS 25.0%
!! TGTTGATGTTAATGGAATTT+TGG - chr3_4:20152098-20152117 Msa0493850:CDS 25.0%
! TTGTAATTGACAACAATCAT+GGG + chr3_4:20150944-20150963 None:intergenic 25.0%
! TTTGTAATTGACAACAATCA+TGG + chr3_4:20150945-20150964 None:intergenic 25.0%
! ATGATTGTTGTCAATTACAA+AGG - chr3_4:20150944-20150963 Msa0493850:CDS 25.0%
! GAAATTGCTGAAACTTATTT+GGG - chr3_4:20151025-20151044 Msa0493850:CDS 25.0%
!! ATTTCATAGAAAGTACAAGA+AGG - chr3_4:20151384-20151403 Msa0493850:CDS 25.0%
! CAATAACAAATTCATGAACT+TGG + chr3_4:20151648-20151667 None:intergenic 25.0%
!!! GTTCATGAATTTGTTATTGT+CGG - chr3_4:20151649-20151668 Msa0493850:CDS 25.0%
!!! AAACATTCTCCTTTTTTGTA+GGG + chr3_4:20151933-20151952 None:intergenic 25.0%
!!! AAAACATTCTCCTTTTTTGT+AGG + chr3_4:20151934-20151953 None:intergenic 25.0%
! TTTGATCAAAACATTACCTT+GGG + chr3_4:20151965-20151984 None:intergenic 25.0%
!!! TGTTTTGATGTTGATGTTAA+TGG - chr3_4:20152090-20152109 Msa0493850:CDS 25.0%
!! TGTTGATGTTAATGGAATTT+TGG - chr3_4:20152098-20152117 Msa0493850:CDS 25.0%
ACCTATTAATCTTTCAGTGT+CGG + chr3_4:20150774-20150793 None:intergenic 30.0%
!!! ATTTTTGGCTGATTGGTTTA+TGG + chr3_4:20150804-20150823 None:intergenic 30.0%
! GAGGTGAAAAATGTTTTTCT+TGG + chr3_4:20150970-20150989 None:intergenic 30.0%
! CTTTAATTTAGACAACACCA+TGG + chr3_4:20151005-20151024 None:intergenic 30.0%
!! CATGGTGTTGTCTAAATTAA+AGG - chr3_4:20151003-20151022 Msa0493850:CDS 30.0%
GGAAATTGCTGAAACTTATT+TGG - chr3_4:20151024-20151043 Msa0493850:CDS 30.0%
AATGTTCTTGTCTTTGATCT+TGG - chr3_4:20151232-20151251 Msa0493850:CDS 30.0%
!!! TGTTACTAGAGCTTTTTTTG+AGG - chr3_4:20151546-20151565 Msa0493850:CDS 30.0%
!! ATGAATTTGTTATTGTCGGA+GGG - chr3_4:20151653-20151672 Msa0493850:CDS 30.0%
!! TTTTGATTCCGAAGAATACT+AGG - chr3_4:20151905-20151924 Msa0493850:CDS 30.0%
CTTTGATCAAAACATTACCT+TGG + chr3_4:20151966-20151985 None:intergenic 30.0%
!! GTTTTGATCAAAGTCTATGA+AGG - chr3_4:20151973-20151992 Msa0493850:CDS 30.0%
! TTGATATTTGGAACCTTTCT+AGG + chr3_4:20152066-20152085 None:intergenic 30.0%
! TGATGTTAATGGAATTTTGG+AGG - chr3_4:20152101-20152120 Msa0493850:CDS 30.0%
ACCTATTAATCTTTCAGTGT+CGG + chr3_4:20150774-20150793 None:intergenic 30.0%
!!! ATTTTTGGCTGATTGGTTTA+TGG + chr3_4:20150804-20150823 None:intergenic 30.0%
! GAGGTGAAAAATGTTTTTCT+TGG + chr3_4:20150970-20150989 None:intergenic 30.0%
! CTTTAATTTAGACAACACCA+TGG + chr3_4:20151005-20151024 None:intergenic 30.0%
!! CATGGTGTTGTCTAAATTAA+AGG - chr3_4:20151003-20151022 Msa0493850:CDS 30.0%
GGAAATTGCTGAAACTTATT+TGG - chr3_4:20151024-20151043 Msa0493850:CDS 30.0%
AATGTTCTTGTCTTTGATCT+TGG - chr3_4:20151232-20151251 Msa0493850:CDS 30.0%
!!! TGTTACTAGAGCTTTTTTTG+AGG - chr3_4:20151546-20151565 Msa0493850:CDS 30.0%
!! ATGAATTTGTTATTGTCGGA+GGG - chr3_4:20151653-20151672 Msa0493850:CDS 30.0%
!! TTTTGATTCCGAAGAATACT+AGG - chr3_4:20151905-20151924 Msa0493850:CDS 30.0%
CTTTGATCAAAACATTACCT+TGG + chr3_4:20151966-20151985 None:intergenic 30.0%
!! GTTTTGATCAAAGTCTATGA+AGG - chr3_4:20151973-20151992 Msa0493850:CDS 30.0%
! TTGATATTTGGAACCTTTCT+AGG + chr3_4:20152066-20152085 None:intergenic 30.0%
! TGATGTTAATGGAATTTTGG+AGG - chr3_4:20152101-20152120 Msa0493850:CDS 30.0%
ACAAGAAAAAGCAAAGCCAT+AGG - chr3_4:20150638-20150657 Msa0493850:CDS 35.0%
ACCGACACTGAAAGATTAAT+AGG - chr3_4:20150770-20150789 Msa0493850:CDS 35.0%
CAAACGTTTGGCATCAAAAA+CGG + chr3_4:20150837-20150856 None:intergenic 35.0%
TTTGATGCCAAACGTTTGAT+TGG - chr3_4:20150839-20150858 Msa0493850:CDS 35.0%
! TTATGTCTTGTTGGACTGAT+TGG + chr3_4:20150880-20150899 None:intergenic 35.0%
AATCAGTCCAACAAGACATA+AGG - chr3_4:20150879-20150898 Msa0493850:CDS 35.0%
! TCCAACAAGACATAAGGTTT+TGG - chr3_4:20150885-20150904 Msa0493850:CDS 35.0%
!! CATAAGGTTTTGGCCTTTTA+AGG - chr3_4:20150895-20150914 Msa0493850:CDS 35.0%
! TGGTTTGGAACAACCTTAAA+AGG + chr3_4:20150911-20150930 None:intergenic 35.0%
AAAGATGCAGGAAAAATTGC+CGG - chr3_4:20151121-20151140 Msa0493850:CDS 35.0%
GATCCTCATCACATTAAAAC+CGG + chr3_4:20151143-20151162 None:intergenic 35.0%
! ATGGTTTGGACAAGAAAATG+TGG - chr3_4:20151194-20151213 Msa0493850:CDS 35.0%
! ACTTTGGGAGATACTCATTT+GGG - chr3_4:20151316-20151335 Msa0493850:CDS 35.0%
AGTGAGAATTCCAAAGCTTT+GGG - chr3_4:20151418-20151437 Msa0493850:CDS 35.0%
AGCTTGATTCACTATGTGAA+GGG - chr3_4:20151506-20151525 Msa0493850:CDS 35.0%
!! CATGAATTTGTTATTGTCGG+AGG - chr3_4:20151652-20151671 Msa0493850:CDS 35.0%
GTCAGTAGTTGTTGAATCTT+CGG + chr3_4:20151691-20151710 None:intergenic 35.0%
GAAGATTCAACAACTACTGA+CGG - chr3_4:20151690-20151709 Msa0493850:CDS 35.0%
ACGGAAATGTTTAGTGTCAA+CGG - chr3_4:20151709-20151728 Msa0493850:CDS 35.0%
! TCAGGATTAATGCTTTTGCA+AGG + chr3_4:20151748-20151767 None:intergenic 35.0%
GCAGCAATATTGAATACTGA+AGG - chr3_4:20151799-20151818 Msa0493850:CDS 35.0%
! GATGTTATGCCTTTTAGTCT+TGG - chr3_4:20151853-20151872 Msa0493850:CDS 35.0%
GTTTCAACACCAAGACTAAA+AGG + chr3_4:20151865-20151884 None:intergenic 35.0%
!! AGTCTTGGTGTTGAAACTAA+AGG - chr3_4:20151868-20151887 Msa0493850:CDS 35.0%
TACTAGGATCCCTACAAAAA+AGG - chr3_4:20151921-20151940 Msa0493850:CDS 35.0%
! ACTAAGGACAACTTTTTGCT+TGG - chr3_4:20152009-20152028 Msa0493850:CDS 35.0%
ATGCTCTTTAGTACCTAGAA+AGG - chr3_4:20152050-20152069 Msa0493850:CDS 35.0%
ACTGCTGAAGATAAGACAAA+AGG - chr3_4:20152126-20152145 Msa0493850:CDS 35.0%
CTGCTGAAGATAAGACAAAA+GGG - chr3_4:20152127-20152146 Msa0493850:CDS 35.0%
GAAGATCATTATCACCAACA+AGG - chr3_4:20152158-20152177 Msa0493850:CDS 35.0%
ATCATTATCACCAACAAGGA+GGG - chr3_4:20152162-20152181 Msa0493850:CDS 35.0%
ATCATTCTTCTCATCTCTTG+AGG + chr3_4:20152198-20152217 None:intergenic 35.0%
AAGAGATGAGAAGAATGATG+AGG - chr3_4:20152199-20152218 Msa0493850:CDS 35.0%
AGAGATGAGAAGAATGATGA+GGG - chr3_4:20152200-20152219 Msa0493850:CDS 35.0%
! TAGTGGAGGAAAGTATAGAA+TGG - chr3_4:20152337-20152356 Msa0493850:CDS 35.0%
ACTCATCAATTTCTGCCAAT+TGG + chr3_4:20152374-20152393 None:intergenic 35.0%
TGAGTTTGAATTCAAGAAGC+AGG - chr3_4:20152389-20152408 Msa0493850:CDS 35.0%
ACAAGAAAAAGCAAAGCCAT+AGG - chr3_4:20150638-20150657 Msa0493850:CDS 35.0%
ACCGACACTGAAAGATTAAT+AGG - chr3_4:20150770-20150789 Msa0493850:CDS 35.0%
CAAACGTTTGGCATCAAAAA+CGG + chr3_4:20150837-20150856 None:intergenic 35.0%
TTTGATGCCAAACGTTTGAT+TGG - chr3_4:20150839-20150858 Msa0493850:CDS 35.0%
! TTATGTCTTGTTGGACTGAT+TGG + chr3_4:20150880-20150899 None:intergenic 35.0%
AATCAGTCCAACAAGACATA+AGG - chr3_4:20150879-20150898 Msa0493850:CDS 35.0%
! TCCAACAAGACATAAGGTTT+TGG - chr3_4:20150885-20150904 Msa0493850:CDS 35.0%
!! CATAAGGTTTTGGCCTTTTA+AGG - chr3_4:20150895-20150914 Msa0493850:CDS 35.0%
! TGGTTTGGAACAACCTTAAA+AGG + chr3_4:20150911-20150930 None:intergenic 35.0%
AAAGATGCAGGAAAAATTGC+CGG - chr3_4:20151121-20151140 Msa0493850:CDS 35.0%
GATCCTCATCACATTAAAAC+CGG + chr3_4:20151143-20151162 None:intergenic 35.0%
! ATGGTTTGGACAAGAAAATG+TGG - chr3_4:20151194-20151213 Msa0493850:CDS 35.0%
! ACTTTGGGAGATACTCATTT+GGG - chr3_4:20151316-20151335 Msa0493850:CDS 35.0%
AGTGAGAATTCCAAAGCTTT+GGG - chr3_4:20151418-20151437 Msa0493850:CDS 35.0%
AGCTTGATTCACTATGTGAA+GGG - chr3_4:20151506-20151525 Msa0493850:CDS 35.0%
!! CATGAATTTGTTATTGTCGG+AGG - chr3_4:20151652-20151671 Msa0493850:CDS 35.0%
GTCAGTAGTTGTTGAATCTT+CGG + chr3_4:20151691-20151710 None:intergenic 35.0%
GAAGATTCAACAACTACTGA+CGG - chr3_4:20151690-20151709 Msa0493850:CDS 35.0%
ACGGAAATGTTTAGTGTCAA+CGG - chr3_4:20151709-20151728 Msa0493850:CDS 35.0%
! TCAGGATTAATGCTTTTGCA+AGG + chr3_4:20151748-20151767 None:intergenic 35.0%
GCAGCAATATTGAATACTGA+AGG - chr3_4:20151799-20151818 Msa0493850:CDS 35.0%
! GATGTTATGCCTTTTAGTCT+TGG - chr3_4:20151853-20151872 Msa0493850:CDS 35.0%
GTTTCAACACCAAGACTAAA+AGG + chr3_4:20151865-20151884 None:intergenic 35.0%
!! AGTCTTGGTGTTGAAACTAA+AGG - chr3_4:20151868-20151887 Msa0493850:CDS 35.0%
TACTAGGATCCCTACAAAAA+AGG - chr3_4:20151921-20151940 Msa0493850:CDS 35.0%
! ACTAAGGACAACTTTTTGCT+TGG - chr3_4:20152009-20152028 Msa0493850:CDS 35.0%
ATGCTCTTTAGTACCTAGAA+AGG - chr3_4:20152050-20152069 Msa0493850:CDS 35.0%
ACTGCTGAAGATAAGACAAA+AGG - chr3_4:20152126-20152145 Msa0493850:CDS 35.0%
CTGCTGAAGATAAGACAAAA+GGG - chr3_4:20152127-20152146 Msa0493850:CDS 35.0%
GAAGATCATTATCACCAACA+AGG - chr3_4:20152158-20152177 Msa0493850:CDS 35.0%
ATCATTATCACCAACAAGGA+GGG - chr3_4:20152162-20152181 Msa0493850:CDS 35.0%
ATCATTCTTCTCATCTCTTG+AGG + chr3_4:20152198-20152217 None:intergenic 35.0%
AAGAGATGAGAAGAATGATG+AGG - chr3_4:20152199-20152218 Msa0493850:CDS 35.0%
AGAGATGAGAAGAATGATGA+GGG - chr3_4:20152200-20152219 Msa0493850:CDS 35.0%
! TAGTGGAGGAAAGTATAGAA+TGG - chr3_4:20152337-20152356 Msa0493850:CDS 35.0%
ACTCATCAATTTCTGCCAAT+TGG + chr3_4:20152374-20152393 None:intergenic 35.0%
TGAGTTTGAATTCAAGAAGC+AGG - chr3_4:20152389-20152408 Msa0493850:CDS 35.0%
AGATCATTCCAAACGACCAA+GGG - chr3_4:20150717-20150736 Msa0493850:CDS 40.0%
!! TAATCTTTCAGTGTCGGTGA+AGG + chr3_4:20150768-20150787 None:intergenic 40.0%
!!! TGTGTGGATTTTTGGCTGAT+TGG + chr3_4:20150811-20150830 None:intergenic 40.0%
!!! AACGGTGTTGTGTGGATTTT+TGG + chr3_4:20150819-20150838 None:intergenic 40.0%
GCCAAAACCTTATGTCTTGT+TGG + chr3_4:20150889-20150908 None:intergenic 40.0%
ATGGGTTTGTCTTTGTGGTT+TGG + chr3_4:20150926-20150945 None:intergenic 40.0%
CAATCATGGGTTTGTCTTTG+TGG + chr3_4:20150931-20150950 None:intergenic 40.0%
ACCTCAAGAGATATCTTCCA+TGG - chr3_4:20150985-20151004 Msa0493850:CDS 40.0%
ACCATGGAAGATATCTCTTG+AGG + chr3_4:20150989-20151008 None:intergenic 40.0%
TGTGAGTTGTTGAAGTAAGC+AGG + chr3_4:20151088-20151107 None:intergenic 40.0%
AGACAAGCTACAAAAGATGC+AGG - chr3_4:20151109-20151128 Msa0493850:CDS 40.0%
!!! TTGCCGGTTTTAATGTGATG+AGG - chr3_4:20151137-20151156 Msa0493850:CDS 40.0%
AGCTGCTATTGCTTATGGTT+TGG - chr3_4:20151180-20151199 Msa0493850:CDS 40.0%
GACAAGAAAATGTGGAGAGA+AGG - chr3_4:20151202-20151221 Msa0493850:CDS 40.0%
GTTCTTGTCTTTGATCTTGG+TGG - chr3_4:20151235-20151254 Msa0493850:CDS 40.0%
CAAATGAGTATCTCCCAAAG+TGG + chr3_4:20151317-20151336 None:intergenic 40.0%
! CACTTTGGGAGATACTCATT+TGG - chr3_4:20151315-20151334 Msa0493850:CDS 40.0%
CAGTGAGAATTCCAAAGCTT+TGG - chr3_4:20151417-20151436 Msa0493850:CDS 40.0%
GTGAGAATTCCAAAGCTTTG+GGG - chr3_4:20151419-20151438 Msa0493850:CDS 40.0%
GAGCTTGATTCACTATGTGA+AGG - chr3_4:20151505-20151524 Msa0493850:CDS 40.0%
!! TGTGGAGAAGTGTTTGATTG+AGG - chr3_4:20151606-20151625 Msa0493850:CDS 40.0%
CATTAATCCTGATGAAGCTG+TGG - chr3_4:20151756-20151775 Msa0493850:CDS 40.0%
GAAGGAGACAAGAAGATTGA+AGG - chr3_4:20151817-20151836 Msa0493850:CDS 40.0%
GATTGAAGGCTTATTGCTGT+TGG - chr3_4:20151831-20151850 Msa0493850:CDS 40.0%
!! CTTGGTGTTGAAACTAAAGG+TGG - chr3_4:20151871-20151890 Msa0493850:CDS 40.0%
GTAGGGATCCTAGTATTCTT+CGG + chr3_4:20151916-20151935 None:intergenic 40.0%
AGGAGAACATGCTAAGACTA+AGG - chr3_4:20151993-20152012 Msa0493850:CDS 40.0%
GATCATTATCACCAACAAGG+AGG - chr3_4:20152161-20152180 Msa0493850:CDS 40.0%
ATGGCTTGACAGAAACCAAT+TGG - chr3_4:20152356-20152375 Msa0493850:CDS 40.0%
TGAATTCAAGAAGCAGGAGT+TGG - chr3_4:20152395-20152414 Msa0493850:CDS 40.0%
AGATCATTCCAAACGACCAA+GGG - chr3_4:20150717-20150736 Msa0493850:CDS 40.0%
!! TAATCTTTCAGTGTCGGTGA+AGG + chr3_4:20150768-20150787 None:intergenic 40.0%
!!! TGTGTGGATTTTTGGCTGAT+TGG + chr3_4:20150811-20150830 None:intergenic 40.0%
!!! AACGGTGTTGTGTGGATTTT+TGG + chr3_4:20150819-20150838 None:intergenic 40.0%
GCCAAAACCTTATGTCTTGT+TGG + chr3_4:20150889-20150908 None:intergenic 40.0%
ATGGGTTTGTCTTTGTGGTT+TGG + chr3_4:20150926-20150945 None:intergenic 40.0%
CAATCATGGGTTTGTCTTTG+TGG + chr3_4:20150931-20150950 None:intergenic 40.0%
ACCTCAAGAGATATCTTCCA+TGG - chr3_4:20150985-20151004 Msa0493850:CDS 40.0%
ACCATGGAAGATATCTCTTG+AGG + chr3_4:20150989-20151008 None:intergenic 40.0%
TGTGAGTTGTTGAAGTAAGC+AGG + chr3_4:20151088-20151107 None:intergenic 40.0%
AGACAAGCTACAAAAGATGC+AGG - chr3_4:20151109-20151128 Msa0493850:CDS 40.0%
!!! TTGCCGGTTTTAATGTGATG+AGG - chr3_4:20151137-20151156 Msa0493850:CDS 40.0%
AGCTGCTATTGCTTATGGTT+TGG - chr3_4:20151180-20151199 Msa0493850:CDS 40.0%
GACAAGAAAATGTGGAGAGA+AGG - chr3_4:20151202-20151221 Msa0493850:CDS 40.0%
GTTCTTGTCTTTGATCTTGG+TGG - chr3_4:20151235-20151254 Msa0493850:CDS 40.0%
CAAATGAGTATCTCCCAAAG+TGG + chr3_4:20151317-20151336 None:intergenic 40.0%
! CACTTTGGGAGATACTCATT+TGG - chr3_4:20151315-20151334 Msa0493850:CDS 40.0%
CAGTGAGAATTCCAAAGCTT+TGG - chr3_4:20151417-20151436 Msa0493850:CDS 40.0%
GTGAGAATTCCAAAGCTTTG+GGG - chr3_4:20151419-20151438 Msa0493850:CDS 40.0%
GAGCTTGATTCACTATGTGA+AGG - chr3_4:20151505-20151524 Msa0493850:CDS 40.0%
!! TGTGGAGAAGTGTTTGATTG+AGG - chr3_4:20151606-20151625 Msa0493850:CDS 40.0%
CATTAATCCTGATGAAGCTG+TGG - chr3_4:20151756-20151775 Msa0493850:CDS 40.0%
GAAGGAGACAAGAAGATTGA+AGG - chr3_4:20151817-20151836 Msa0493850:CDS 40.0%
GATTGAAGGCTTATTGCTGT+TGG - chr3_4:20151831-20151850 Msa0493850:CDS 40.0%
!! CTTGGTGTTGAAACTAAAGG+TGG - chr3_4:20151871-20151890 Msa0493850:CDS 40.0%
GTAGGGATCCTAGTATTCTT+CGG + chr3_4:20151916-20151935 None:intergenic 40.0%
AGGAGAACATGCTAAGACTA+AGG - chr3_4:20151993-20152012 Msa0493850:CDS 40.0%
GATCATTATCACCAACAAGG+AGG - chr3_4:20152161-20152180 Msa0493850:CDS 40.0%
ATGGCTTGACAGAAACCAAT+TGG - chr3_4:20152356-20152375 Msa0493850:CDS 40.0%
TGAATTCAAGAAGCAGGAGT+TGG - chr3_4:20152395-20152414 Msa0493850:CDS 40.0%
!! AAAGCCATAGGCATTGACCT+TGG - chr3_4:20150650-20150669 Msa0493850:CDS 45.0%
TGTACCAAGGTCAATGCCTA+TGG + chr3_4:20150657-20150676 None:intergenic 45.0%
! TACAGCTGTAGCTTGTACCA+AGG + chr3_4:20150670-20150689 None:intergenic 45.0%
GAGATCATTCCAAACGACCA+AGG - chr3_4:20150716-20150735 Msa0493850:CDS 45.0%
!! GCATCAAAAACGGTGTTGTG+TGG + chr3_4:20150827-20150846 None:intergenic 45.0%
GCGATGACCAATCAAACGTT+TGG + chr3_4:20150849-20150868 None:intergenic 45.0%
TAAGCAATAGCAGCTGCAGT+AGG + chr3_4:20151175-20151194 None:intergenic 45.0%
ACTGCAGCTGCTATTGCTTA+TGG - chr3_4:20151175-20151194 Msa0493850:CDS 45.0%
CTTGTCTTTGATCTTGGTGG+TGG - chr3_4:20151238-20151257 Msa0493850:CDS 45.0%
TCCTTAGTGACTGTTGACGA+AGG - chr3_4:20151274-20151293 Msa0493850:CDS 45.0%
TCCTTCGTCAACAGTCACTA+AGG + chr3_4:20151278-20151297 None:intergenic 45.0%
TGTTGACGAAGGAATGTTCC+AGG - chr3_4:20151285-20151304 Msa0493850:CDS 45.0%
CGAAGGAATGTTCCAGGTTA+AGG - chr3_4:20151291-20151310 Msa0493850:CDS 45.0%
! TTCCAGGTTAAGGCCACTTT+GGG - chr3_4:20151301-20151320 Msa0493850:CDS 45.0%
TTGGGAGATACTCATTTGGG+TGG - chr3_4:20151319-20151338 Msa0493850:CDS 45.0%
!! AGAATTCCAAAGCTTTGGGG+AGG - chr3_4:20151422-20151441 Msa0493850:CDS 45.0%
TCTTAACCTCCCCAAAGCTT+TGG + chr3_4:20151431-20151450 None:intergenic 45.0%
! CAGAAAGTGCATGAAGACTG+TGG - chr3_4:20151588-20151607 Msa0493850:CDS 45.0%
! AATGTTTTCTCGACAGCCCA+AGG - chr3_4:20151946-20151965 Msa0493850:CDS 45.0%
TTATCACCAACAAGGAGGGA+AGG - chr3_4:20152166-20152185 Msa0493850:CDS 45.0%
ATGAAATGAGGGAGAGAGTG+AGG - chr3_4:20152301-20152320 Msa0493850:CDS 45.0%
GAGAGTGAGGAAGCTTGAAA+AGG - chr3_4:20152314-20152333 Msa0493850:CDS 45.0%
GAGGAAGCTTGAAAAGGTAG+TGG - chr3_4:20152320-20152339 Msa0493850:CDS 45.0%
! GAAGCTTGAAAAGGTAGTGG+AGG - chr3_4:20152323-20152342 Msa0493850:CDS 45.0%
!! AAAGCCATAGGCATTGACCT+TGG - chr3_4:20150650-20150669 Msa0493850:CDS 45.0%
TGTACCAAGGTCAATGCCTA+TGG + chr3_4:20150657-20150676 None:intergenic 45.0%
! TACAGCTGTAGCTTGTACCA+AGG + chr3_4:20150670-20150689 None:intergenic 45.0%
GAGATCATTCCAAACGACCA+AGG - chr3_4:20150716-20150735 Msa0493850:CDS 45.0%
!! GCATCAAAAACGGTGTTGTG+TGG + chr3_4:20150827-20150846 None:intergenic 45.0%
GCGATGACCAATCAAACGTT+TGG + chr3_4:20150849-20150868 None:intergenic 45.0%
TAAGCAATAGCAGCTGCAGT+AGG + chr3_4:20151175-20151194 None:intergenic 45.0%
ACTGCAGCTGCTATTGCTTA+TGG - chr3_4:20151175-20151194 Msa0493850:CDS 45.0%
CTTGTCTTTGATCTTGGTGG+TGG - chr3_4:20151238-20151257 Msa0493850:CDS 45.0%
TCCTTAGTGACTGTTGACGA+AGG - chr3_4:20151274-20151293 Msa0493850:CDS 45.0%
TCCTTCGTCAACAGTCACTA+AGG + chr3_4:20151278-20151297 None:intergenic 45.0%
TGTTGACGAAGGAATGTTCC+AGG - chr3_4:20151285-20151304 Msa0493850:CDS 45.0%
CGAAGGAATGTTCCAGGTTA+AGG - chr3_4:20151291-20151310 Msa0493850:CDS 45.0%
! TTCCAGGTTAAGGCCACTTT+GGG - chr3_4:20151301-20151320 Msa0493850:CDS 45.0%
TTGGGAGATACTCATTTGGG+TGG - chr3_4:20151319-20151338 Msa0493850:CDS 45.0%
!! AGAATTCCAAAGCTTTGGGG+AGG - chr3_4:20151422-20151441 Msa0493850:CDS 45.0%
TCTTAACCTCCCCAAAGCTT+TGG + chr3_4:20151431-20151450 None:intergenic 45.0%
! CAGAAAGTGCATGAAGACTG+TGG - chr3_4:20151588-20151607 Msa0493850:CDS 45.0%
! AATGTTTTCTCGACAGCCCA+AGG - chr3_4:20151946-20151965 Msa0493850:CDS 45.0%
TTATCACCAACAAGGAGGGA+AGG - chr3_4:20152166-20152185 Msa0493850:CDS 45.0%
ATGAAATGAGGGAGAGAGTG+AGG - chr3_4:20152301-20152320 Msa0493850:CDS 45.0%
GAGAGTGAGGAAGCTTGAAA+AGG - chr3_4:20152314-20152333 Msa0493850:CDS 45.0%
GAGGAAGCTTGAAAAGGTAG+TGG - chr3_4:20152320-20152339 Msa0493850:CDS 45.0%
! GAAGCTTGAAAAGGTAGTGG+AGG - chr3_4:20152323-20152342 Msa0493850:CDS 45.0%
GCTACAGCTGTATTGCAGTG+TGG - chr3_4:20150678-20150697 Msa0493850:CDS 50.0%
CAACGTAAGAAGGGGTGACA+CGG + chr3_4:20150745-20150764 None:intergenic 50.0%
GGTGAAGGCAACGTAAGAAG+GGG + chr3_4:20150753-20150772 None:intergenic 50.0%
CGGTGAAGGCAACGTAAGAA+GGG + chr3_4:20150754-20150773 None:intergenic 50.0%
TCGGTGAAGGCAACGTAAGA+AGG + chr3_4:20150755-20150774 None:intergenic 50.0%
GTTCCAGGTTAAGGCCACTT+TGG - chr3_4:20151300-20151319 Msa0493850:CDS 50.0%
CAGCATGCGAGAAAGCAAAG+AGG - chr3_4:20151452-20151471 Msa0493850:CDS 50.0%
CCATAAGCCACAGCTTCATC+AGG + chr3_4:20151766-20151785 None:intergenic 50.0%
CCTGATGAAGCTGTGGCTTA+TGG - chr3_4:20151763-20151782 Msa0493850:CDS 50.0%
ACTCAACCTTCCCTCCTTGT+TGG + chr3_4:20152175-20152194 None:intergenic 50.0%
ATCCTCTGCCTTATACCGCT+CGG + chr3_4:20152229-20152248 None:intergenic 50.0%
GTATAAGGCAGAGGATGAGG+AGG - chr3_4:20152233-20152252 Msa0493850:CDS 50.0%
GGATGAGGAGGTTAGGAAGA+AGG - chr3_4:20152245-20152264 Msa0493850:CDS 50.0%
GCTACAGCTGTATTGCAGTG+TGG - chr3_4:20150678-20150697 Msa0493850:CDS 50.0%
CAACGTAAGAAGGGGTGACA+CGG + chr3_4:20150745-20150764 None:intergenic 50.0%
GGTGAAGGCAACGTAAGAAG+GGG + chr3_4:20150753-20150772 None:intergenic 50.0%
CGGTGAAGGCAACGTAAGAA+GGG + chr3_4:20150754-20150773 None:intergenic 50.0%
TCGGTGAAGGCAACGTAAGA+AGG + chr3_4:20150755-20150774 None:intergenic 50.0%
GTTCCAGGTTAAGGCCACTT+TGG - chr3_4:20151300-20151319 Msa0493850:CDS 50.0%
CAGCATGCGAGAAAGCAAAG+AGG - chr3_4:20151452-20151471 Msa0493850:CDS 50.0%
CCATAAGCCACAGCTTCATC+AGG + chr3_4:20151766-20151785 None:intergenic 50.0%
CCTGATGAAGCTGTGGCTTA+TGG - chr3_4:20151763-20151782 Msa0493850:CDS 50.0%
ACTCAACCTTCCCTCCTTGT+TGG + chr3_4:20152175-20152194 None:intergenic 50.0%
ATCCTCTGCCTTATACCGCT+CGG + chr3_4:20152229-20152248 None:intergenic 50.0%
GTATAAGGCAGAGGATGAGG+AGG - chr3_4:20152233-20152252 Msa0493850:CDS 50.0%
GGATGAGGAGGTTAGGAAGA+AGG - chr3_4:20152245-20152264 Msa0493850:CDS 50.0%
ACACGGTTCCCTTGGTCGTT+TGG + chr3_4:20150728-20150747 None:intergenic 55.0%
! CTCCCAAAGTGGCCTTAACC+TGG + chr3_4:20151306-20151325 None:intergenic 55.0%
GAATGATGAGGGATGCCGAG+CGG - chr3_4:20152211-20152230 Msa0493850:CDS 55.0%
GCGGTATAAGGCAGAGGATG+AGG - chr3_4:20152230-20152249 Msa0493850:CDS 55.0%
AGGCAGAGGATGAGGAGGTT+AGG - chr3_4:20152238-20152257 Msa0493850:CDS 55.0%
ACACGGTTCCCTTGGTCGTT+TGG + chr3_4:20150728-20150747 None:intergenic 55.0%
! CTCCCAAAGTGGCCTTAACC+TGG + chr3_4:20151306-20151325 None:intergenic 55.0%
GAATGATGAGGGATGCCGAG+CGG - chr3_4:20152211-20152230 Msa0493850:CDS 55.0%
GCGGTATAAGGCAGAGGATG+AGG - chr3_4:20152230-20152249 Msa0493850:CDS 55.0%
AGGCAGAGGATGAGGAGGTT+AGG - chr3_4:20152238-20152257 Msa0493850:CDS 55.0%
AAGGGGTGACACGGTTCCCT+TGG + chr3_4:20150736-20150755 None:intergenic 60.0%
GAGGGATGCCGAGCGGTATA+AGG - chr3_4:20152218-20152237 Msa0493850:CDS 60.0%
TGCCGAGCGGTATAAGGCAG+AGG - chr3_4:20152224-20152243 Msa0493850:CDS 60.0%
AAGGGGTGACACGGTTCCCT+TGG + chr3_4:20150736-20150755 None:intergenic 60.0%
GAGGGATGCCGAGCGGTATA+AGG - chr3_4:20152218-20152237 Msa0493850:CDS 60.0%
TGCCGAGCGGTATAAGGCAG+AGG - chr3_4:20152224-20152243 Msa0493850:CDS 60.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 20150632 20152440 20150632 ID=Msa0493850;Name=Msa0493850
chr3_4 mRNA 20150632 20152440 20150632 ID=Msa0493850-mRNA-1;Parent=Msa0493850;Name=Msa0493850-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|602
chr3_4 exon 20150632 20152440 20150632 ID=Msa0493850-mRNA-1:exon:5322;Parent=Msa0493850-mRNA-1
chr3_4 CDS 20150632 20152440 20150632 ID=Msa0493850-mRNA-1:cds;Parent=Msa0493850-mRNA-1
Gene Sequence

>Msa0493850

ATGGCAACAAGAAAAAGCAAAGCCATAGGCATTGACCTTGGTACAAGCTACAGCTGTATTGCAGTGTGGCGAAACAATTGTGTTGAGATCATTCCAAACGACCAAGGGAACCGTGTCACCCCTTCTTACGTTGCCTTCACCGACACTGAAAGATTAATAGGTGATGCTGCCATAAACCAATCAGCCAAAAATCCACACAACACCGTTTTTGATGCCAAACGTTTGATTGGTCATCGCTTCTCTGACCAATCAGTCCAACAAGACATAAGGTTTTGGCCTTTTAAGGTTGTTCCAAACCACAAAGACAAACCCATGATTGTTGTCAATTACAAAGGCCAAGAAAAACATTTTTCACCTCAAGAGATATCTTCCATGGTGTTGTCTAAATTAAAGGAAATTGCTGAAACTTATTTGGGTCATGATCATGAAGTGAACAAAGCTGTTATCACTGTTCCTGCTTACTTCAACAACTCACAGAGACAAGCTACAAAAGATGCAGGAAAAATTGCCGGTTTTAATGTGATGAGGATCATCAACGAGCCTACTGCAGCTGCTATTGCTTATGGTTTGGACAAGAAAATGTGGAGAGAAGGCGAGAAGAATGTTCTTGTCTTTGATCTTGGTGGTGGAACTTTTGATGTTTCCTTAGTGACTGTTGACGAAGGAATGTTCCAGGTTAAGGCCACTTTGGGAGATACTCATTTGGGTGGTGTTGATTTTGATAACAATTTGGTGAACCATCTTGTTGAATTATTTCATAGAAAGTACAAGAAGGATTTGAAGATCAGTGAGAATTCCAAAGCTTTGGGGAGGTTAAGATCAGCATGCGAGAAAGCAAAGAGGTTACTTTCTTCATCATCACAAACAGTTGTTGAGCTTGATTCACTATGTGAAGGGATTGATCTACATTTCACTGTTACTAGAGCTTTTTTTGAGGAAATAAACAAGGATTTGTTCAGAAAGTGCATGAAGACTGTGGAGAAGTGTTTGATTGAGGCAAAAATTAGTAAAAGCCAAGTTCATGAATTTGTTATTGTCGGAGGGTCTACTAGAATTCCGAAGATTCAACAACTACTGACGGAAATGTTTAGTGTCAACGGCGAAGTCAAAGAGCCTTGCAAAAGCATTAATCCTGATGAAGCTGTGGCTTATGGCGCAGCAGTTCAAGCAGCAATATTGAATACTGAAGGAGACAAGAAGATTGAAGGCTTATTGCTGTTGGATGTTATGCCTTTTAGTCTTGGTGTTGAAACTAAAGGTGGTGTCATGTCTGTTTTGATTCCGAAGAATACTAGGATCCCTACAAAAAAGGAGAATGTTTTCTCGACAGCCCAAGGTAATGTTTTGATCAAAGTCTATGAAGGAGAACATGCTAAGACTAAGGACAACTTTTTGCTTGGTAAATTTGAGCTCTCAGAATGCTCTTTAGTACCTAGAAAGGTTCCAAATATCAATATTTGTTTTGATGTTGATGTTAATGGAATTTTGGAGGTTACTGCTGAAGATAAGACAAAAGGGTTGAAACAGAAGATCATTATCACCAACAAGGAGGGAAGGTTGAGTCCTCAAGAGATGAGAAGAATGATGAGGGATGCCGAGCGGTATAAGGCAGAGGATGAGGAGGTTAGGAAGAAGGTGAAAGCAAAGAATTTGTTTGAGAATTATGTTTATGAAATGAGGGAGAGAGTGAGGAAGCTTGAAAAGGTAGTGGAGGAAAGTATAGAATGGCTTGACAGAAACCAATTGGCAGAAATTGATGAGTTTGAATTCAAGAAGCAGGAGTTGGAAAATAGTGTGAAGTTTCTTTAA
Protein sequence

>Msa0493850

MATRKSKAIGIDLGTSYSCIAVWRNNCVEIIPNDQGNRVTPSYVAFTDTERLIGDAAINQSAKNPHNTVFDAKRLIGHRFSDQSVQQDIRFWPFKVVPNHKDKPMIVVNYKGQEKHFSPQEISSMVLSKLKEIAETYLGHDHEVNKAVITVPAYFNNSQRQATKDAGKIAGFNVMRIINEPTAAAIAYGLDKKMWREGEKNVLVFDLGGGTFDVSLVTVDEGMFQVKATLGDTHLGGVDFDNNLVNHLVELFHRKYKKDLKISENSKALGRLRSACEKAKRLLSSSSQTVVELDSLCEGIDLHFTVTRAFFEEINKDLFRKCMKTVEKCLIEAKISKSQVHEFVIVGGSTRIPKIQQLLTEMFSVNGEVKEPCKSINPDEAVAYGAAVQAAILNTEGDKKIEGLLLLDVMPFSLGVETKGGVMSVLIPKNTRIPTKKENVFSTAQGNVLIKVYEGEHAKTKDNFLLGKFELSECSLVPRKVPNINICFDVDVNGILEVTAEDKTKGLKQKIIITNKEGRLSPQEMRRMMRDAERYKAEDEEVRKKVKAKNLFENYVYEMRERVRKLEKVVEESIEWLDRNQLAEIDEFEFKKQELENSVKFL*