AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0399210

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0399210 MtrunA17_Chr2g0284681 92.066 605 43 2 1 602 1 603 0.0 1140
Msa0399210 MtrunA17_Chr4g0073741 67.863 613 172 6 1 596 1 605 0.0 852
Msa0399210 MtrunA17_Chr4g0031301 66.667 606 177 6 8 596 9 606 0.0 816
Msa0399210 MtrunA17_Chr4g0031321 66.337 606 179 6 8 596 9 606 0.0 811
Msa0399210 MtrunA17_Chr7g0223181 65.898 607 182 6 8 597 9 607 0.0 807
Msa0399210 MtrunA17_Chr7g0262821 65.512 606 184 6 8 596 9 606 0.0 805
Msa0399210 MtrunA17_Chr7g0223291 65.677 606 183 6 8 596 9 606 0.0 800
Msa0399210 MtrunA17_Chr6g0462681 54.833 600 245 6 8 590 9 599 0.0 662
Msa0399210 MtrunA17_Chr6g0462601 55.500 600 241 6 8 590 9 599 0.0 649
Msa0399210 MtrunA17_Chr8g0388671 55.092 599 238 8 2 581 32 618 0.0 648
Msa0399210 MtrunA17_Chr7g0223741 58.363 562 219 7 5 561 6 557 0.0 648
Msa0399210 MtrunA17_Chr5g0427901 53.920 625 248 9 8 602 9 623 0.0 644
Msa0399210 MtrunA17_Chr8g0388761 54.348 598 242 8 3 581 33 618 0.0 644
Msa0399210 MtrunA17_Chr7g0223751 57.066 559 224 9 8 561 9 556 0.0 639
Msa0399210 MtrunA17_Chr7g0223711 56.584 562 229 7 5 561 6 557 0.0 636
Msa0399210 MtrunA17_Chr7g0221661 54.516 609 248 9 8 597 9 607 0.0 635
Msa0399210 MtrunA17_Chr2g0298851 54.045 618 254 10 5 602 6 613 0.0 632
Msa0399210 MtrunA17_Chr7g0233311 57.194 563 227 6 8 565 9 562 0.0 624
Msa0399210 MtrunA17_Chr2g0293581 53.234 603 253 9 7 589 9 602 0.0 623
Msa0399210 MtrunA17_Chr7g0223731 56.228 562 230 8 5 561 6 556 0.0 623
Msa0399210 MtrunA17_Chr8g0350731 56.887 559 225 8 8 561 37 584 0.0 623
Msa0399210 MtrunA17_Chr7g0223721 52.846 615 260 10 8 602 9 613 0.0 617
Msa0399210 MtrunA17_Chr6g0462771 51.901 605 264 7 5 589 6 603 0.0 615
Msa0399210 MtrunA17_Chr7g0223701 53.084 616 254 9 8 597 9 615 0.0 615
Msa0399210 MtrunA17_Chr7g0223801 52.053 609 261 10 8 597 9 605 0.0 613
Msa0399210 MtrunA17_Chr6g0462711 52.483 604 263 7 5 589 7 605 0.0 613
Msa0399210 MtrunA17_Chr7g0223781 55.300 566 236 8 8 568 9 562 0.0 612
Msa0399210 MtrunA17_Chr6g0462641 52.303 608 266 7 1 589 1 603 0.0 612
Msa0399210 MtrunA17_Chr7g0225671 53.215 622 260 11 1 602 9 619 0.0 611
Msa0399210 MtrunA17_Chr7g0223621 54.804 562 238 8 8 564 9 559 0.0 610
Msa0399210 MtrunA17_Chr7g0223031 54.804 562 238 8 5 561 6 556 0.0 610
Msa0399210 MtrunA17_Chr2g0287631 52.195 615 265 9 8 602 9 614 0.0 603
Msa0399210 MtrunA17_Chr7g0223851 52.504 619 262 11 5 602 15 622 0.0 602
Msa0399210 MtrunA17_Chr7g0248131 55.575 574 229 12 8 573 9 564 0.0 601
Msa0399210 MtrunA17_Chr2g0298861 51.438 626 262 12 9 602 10 625 0.0 601
Msa0399210 MtrunA17_Chr4g0040931 51.707 615 267 10 8 602 9 613 0.0 600
Msa0399210 MtrunA17_Chr7g0223631 54.025 559 227 10 8 561 9 542 0.0 586
Msa0399210 MtrunA17_Chr7g0248151 55.536 560 228 9 8 559 9 555 0.0 586
Msa0399210 MtrunA17_Chr7g0233301 54.270 562 218 6 8 567 9 533 0.0 581
Msa0399210 MtrunA17_Chr2g0296391 55.639 532 222 6 4 530 5 527 0.0 580
Msa0399210 MtrunA17_Chr7g0223821 50.965 622 270 10 5 602 6 616 0.0 571
Msa0399210 MtrunA17_Chr7g0217971 50.749 601 242 10 8 589 9 574 0.0 571
Msa0399210 MtrunA17_Chr7g0215261 52.566 565 217 8 7 565 8 527 0.0 554
Msa0399210 MtrunA17_Chr6g0462611 51.913 549 236 7 59 590 10 547 0.0 548
Msa0399210 MtrunA17_Chr8g0390821 46.818 660 256 13 8 597 11 645 0.0 548
Msa0399210 MtrunA17_Chr4g0012541 49.111 619 274 12 5 602 6 604 0.0 547
Msa0399210 MtrunA17_Chr7g0223831 52.120 566 228 9 5 565 6 533 0.0 545
Msa0399210 MtrunA17_Chr2g0331381 52.037 540 188 8 9 546 7 477 0.0 543
Msa0399210 MtrunA17_Chr7g0223541 53.722 497 217 6 71 564 31 517 8.13e-180 521
Msa0399210 MtrunA17_Chr7g0228281 47.078 616 251 10 7 602 69 629 3.56e-178 519
Msa0399210 MtrunA17_Chr2g0293601 52.907 516 201 7 8 521 9 484 1.96e-177 511
Msa0399210 MtrunA17_Chr7g0223681 54.088 477 205 7 88 561 2 467 9.86e-175 506
Msa0399210 MtrunA17_Chr2g0299921 66.067 389 122 4 8 396 1029 1407 4.41e-167 520
Msa0399210 MtrunA17_Chr2g0299921 65.464 388 123 6 8 394 1413 1790 7.40e-154 484
Msa0399210 MtrunA17_Chr2g0299821 60.391 409 147 6 9 411 10 409 6.57e-166 482
Msa0399210 MtrunA17_Chr2g0299821 41.429 140 59 3 425 541 343 482 8.57e-22 99.0
Msa0399210 MtrunA17_Chr2g0293561 62.032 374 132 4 8 379 9 374 2.07e-164 474
Msa0399210 MtrunA17_Chr2g0299851 63.566 387 131 5 8 394 1038 1414 1.41e-162 501
Msa0399210 MtrunA17_Chr2g0279901 45.868 593 276 16 9 583 57 622 3.24e-161 476
Msa0399210 MtrunA17_Chr4g0049501 44.444 603 295 14 6 591 51 630 2.61e-157 466
Msa0399210 MtrunA17_Chr2g0299791 63.307 387 132 5 8 394 1042 1418 1.16e-155 483
Msa0399210 MtrunA17_Chr6g0462861 46.060 571 262 14 8 563 9 548 2.60e-152 453
Msa0399210 MtrunA17_Chr1g0166551 43.092 608 209 11 8 597 5 493 1.24e-149 441
Msa0399210 MtrunA17_Chr2g0299881 60.614 391 140 6 8 394 1038 1418 7.36e-147 460
Msa0399210 MtrunA17_Chr2g0299881 60.606 66 22 1 184 249 1414 1475 8.69e-16 81.6
Msa0399210 MtrunA17_Chr2g0277021 44.923 581 286 14 7 575 70 628 2.52e-146 439
Msa0399210 MtrunA17_Chr2g0293271 47.640 445 149 6 20 459 43 408 3.54e-130 387
Msa0399210 MtrunA17_Chr1g0151991 61.724 290 105 3 5 292 6 291 3.40e-124 368
Msa0399210 MtrunA17_Chr7g0245521 64.684 269 89 3 8 274 9 273 1.29e-118 353
Msa0399210 MtrunA17_Chr6g0462631 47.941 340 126 5 83 422 9 297 1.64e-95 294
Msa0399210 MtrunA17_Chr6g0462751 42.659 361 140 5 61 419 9 304 1.55e-89 280
Msa0399210 MtrunA17_Chr7g0223761 38.874 373 167 5 240 597 3 329 1.50e-79 254
Msa0399210 MtrunA17_Chr7g0223671 59.375 192 76 1 71 262 8 197 9.51e-78 245
Msa0399210 MtrunA17_Chr1g0188601 61.658 193 70 2 91 283 3 191 1.67e-76 241
Msa0399210 MtrunA17_Chr4g0004331 33.632 446 272 6 9 441 4 438 1.49e-74 254
Msa0399210 MtrunA17_Chr5g0394391 31.755 507 316 12 8 498 29 521 3.21e-74 248
Msa0399210 MtrunA17_Chr2g0331901 31.656 477 300 8 9 470 4 469 1.61e-73 252
Msa0399210 MtrunA17_Chr7g0245531 43.689 309 151 6 306 597 2 304 5.90e-72 234
Msa0399210 MtrunA17_Chr6g0462791 60.000 180 67 3 105 284 1 175 1.28e-67 218
Msa0399210 MtrunA17_Chr5g0444001 31.111 450 290 7 9 452 4 439 1.98e-66 226
Msa0399210 MtrunA17_Chr3g0098761 29.365 504 334 8 9 502 4 495 4.02e-66 230
Msa0399210 MtrunA17_Chr7g0223771 64.286 154 53 1 91 244 2 153 3.51e-64 208
Msa0399210 MtrunA17_Chr1g0152001 40.264 303 157 7 317 602 2 297 3.56e-64 213
Msa0399210 MtrunA17_Chr4g0060241 30.315 508 316 12 9 500 21 506 1.03e-57 202
Msa0399210 MtrunA17_Chr4g0064621 31.879 527 326 13 8 512 140 655 3.04e-57 204
Msa0399210 MtrunA17_Chr1g0188611 46.939 245 121 5 323 564 1 239 1.09e-54 187
Msa0399210 MtrunA17_Chr7g0223861 65.414 133 44 1 75 207 4 134 2.41e-54 181
Msa0399210 MtrunA17_Chr4g0046881 60.403 149 52 3 128 276 10 151 5.55e-51 172
Msa0399210 MtrunA17_Chr3g0120101 26.177 531 348 11 6 502 24 544 2.32e-50 187
Msa0399210 MtrunA17_Chr6g0462701 51.765 170 54 3 102 271 27 168 8.85e-46 159
Msa0399210 MtrunA17_Chr5g0394231 40.260 231 83 5 71 300 5 181 5.83e-45 157
Msa0399210 MtrunA17_Chr5g0442171 32.845 341 189 11 169 491 1 319 9.07e-44 159
Msa0399210 MtrunA17_Chr6g0462691 51.370 146 64 2 323 465 1 142 1.30e-42 150
Msa0399210 MtrunA17_Chr5g0394221 35.556 270 160 6 299 563 68 328 2.83e-42 155
Msa0399210 MtrunA17_Chr6g0481391 53.226 124 53 2 35 158 13 131 1.10e-34 127
Msa0399210 MtrunA17_Chr2g0284661 62.500 96 33 1 360 452 48 143 4.57e-30 115
Msa0399210 MtrunA17_Chr2g0284661 81.818 55 10 0 224 278 5 59 2.83e-23 96.3
Msa0399210 MtrunA17_Chr4g0046871 54.717 106 43 2 336 441 5 105 1.32e-29 112
Msa0399210 MtrunA17_Chr6g0462621 70.423 71 20 1 1 70 1 71 3.50e-28 107
Msa0399210 MtrunA17_Chr7g0245541 34.896 192 107 3 423 597 1 191 1.79e-27 110
Msa0399210 MtrunA17_Chr7g0223691 70.769 65 17 1 8 70 9 73 4.12e-25 99.0
Msa0399210 MtrunA17_Chr6g0481381 62.162 74 28 0 175 248 1 74 6.49e-25 102
Msa0399210 MtrunA17_Chr1g0188591 64.706 68 22 1 5 70 6 73 8.13e-24 95.9
Msa0399210 MtrunA17_Chr7g0223791 52.439 82 31 2 53 134 18 91 2.39e-19 86.3
Msa0399210 MtrunA17_Chr7g0223791 37.975 79 49 0 486 564 90 168 3.21e-12 65.5
Msa0399210 MtrunA17_Chr7g0223881 48.052 77 39 1 289 365 9 84 1.01e-17 78.2
Msa0399210 MtrunA17_Chr6g0462801 51.515 66 31 1 323 387 1 66 2.66e-15 71.2
Msa0399210 MtrunA17_Chr7g0221651 40.698 86 49 1 214 299 1 84 9.20e-15 70.1
Msa0399210 MtrunA17_Chr4g0064651 38.462 91 53 1 404 491 30 120 1.20e-14 70.9
Msa0399210 MtrunA17_Chr2g0299891 63.636 44 16 0 317 360 2 45 8.21e-14 66.6
Msa0399210 MtrunA17_Chr5g0442161 30.065 153 100 4 8 156 28 177 5.74e-13 67.8
Msa0399210 MtrunA17_Chr2g0299941 60.000 55 19 1 405 456 23 77 1.06e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0399210 AT1G16030.1 66.558 613 180 6 1 596 1 605 0.0 827
Msa0399210 AT3G12580.1 66.557 607 178 6 8 597 9 607 0.0 810
Msa0399210 AT5G02500.1 65.733 607 183 6 8 597 9 607 0.0 804
Msa0399210 AT3G09440.1 65.733 607 183 6 8 597 9 607 0.0 796
Msa0399210 AT3G09440.4 65.733 607 183 6 8 597 9 607 0.0 796
Msa0399210 AT3G09440.2 65.733 607 183 6 8 597 9 607 0.0 796
Msa0399210 AT3G09440.3 65.733 607 183 6 8 597 9 607 0.0 796
Msa0399210 AT5G02490.1 64.909 607 188 6 8 597 9 607 0.0 791
Msa0399210 AT1G56410.1 64.628 605 191 7 8 597 9 605 0.0 782
Msa0399210 AT5G42020.1 55.212 614 244 8 2 596 31 632 0.0 666
Msa0399210 AT5G42020.3 55.212 614 244 8 2 596 36 637 0.0 665
Msa0399210 AT5G28540.1 55.049 614 245 8 2 596 31 632 0.0 663
Msa0399210 AT1G09080.1 55.611 606 239 7 9 596 53 646 0.0 648
Msa0399210 AT1G09080.2 55.611 606 239 7 9 596 43 636 0.0 648
Msa0399210 AT5G42020.2 51.333 600 234 11 2 596 31 577 0.0 578
Msa0399210 AT5G02500.2 69.797 394 111 4 8 401 9 394 0.0 566
Msa0399210 AT5G02500.2 45.652 92 36 1 520 597 386 477 8.97e-16 80.9
Msa0399210 AT5G09590.1 45.812 585 278 15 9 577 60 621 2.57e-156 464
Msa0399210 AT4G37910.1 46.140 570 269 13 9 563 55 601 3.76e-151 451
Msa0399210 AT4G37910.2 46.140 570 269 13 9 563 55 601 3.76e-151 451
Msa0399210 AT5G49910.1 44.062 581 291 14 7 575 79 637 1.18e-144 436
Msa0399210 AT4G24280.1 44.062 581 291 14 7 575 79 637 3.90e-144 434
Msa0399210 AT1G79930.2 33.857 446 271 6 9 441 4 438 2.61e-78 264
Msa0399210 AT1G79930.1 33.857 446 271 6 9 441 4 438 6.94e-78 263
Msa0399210 AT1G79920.3 33.857 446 271 6 9 441 4 438 2.12e-77 260
Msa0399210 AT1G79920.2 33.857 446 271 6 9 441 4 438 2.12e-77 260
Msa0399210 AT1G79920.4 33.857 446 271 6 9 441 4 438 2.12e-77 260
Msa0399210 AT1G79920.1 33.857 446 271 6 9 441 4 438 2.50e-77 260
Msa0399210 AT1G11660.4 28.111 651 393 16 9 602 4 636 5.31e-72 246
Msa0399210 AT1G11660.3 28.111 651 393 16 9 602 4 636 5.31e-72 246
Msa0399210 AT1G11660.1 28.111 651 393 16 9 602 4 636 5.31e-72 246
Msa0399210 AT2G32120.1 30.077 522 322 13 8 512 30 525 3.06e-65 224
Msa0399210 AT2G32120.2 30.077 522 322 13 8 512 30 525 3.06e-65 224
Msa0399210 AT1G11660.2 27.922 616 369 16 44 602 2 599 4.68e-63 221
Msa0399210 AT4G16660.1 25.934 455 304 8 5 436 23 467 1.77e-47 179
Msa0399210 AT4G16660.2 25.934 455 304 8 5 436 47 491 1.87e-47 179

Find 119 sgRNAs with CRISPR-Local

Find 288 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAAATTGCTGAAACTTATT+TGG 0.082085 3_2:-21718058 Msa0399210:CDS
CATGGTGTTGTCTAAATTAA+AGG 0.157364 3_2:-21718079 Msa0399210:CDS
TCAAAACAAATATTGATATT+TGG 0.181030 3_2:+21717007 None:intergenic
TTGATATTTGGAACCTTTCT+AGG 0.182799 3_2:+21717019 None:intergenic
ACTGCAGCTGCTATTGCTTA+TGG 0.208608 3_2:-21717907 Msa0399210:CDS
TGGTTTGGAACAACCTTAAA+AGG 0.212043 3_2:+21718174 None:intergenic
TGTTGATGTTAATGGAATTT+TGG 0.222928 3_2:-21716984 Msa0399210:CDS
GTTTCAACACCAAGACTAAA+AGG 0.273894 3_2:+21717220 None:intergenic
CAATCATGGGTTTGTCTTTG+TGG 0.290878 3_2:+21718154 None:intergenic
CAGTGAGAATTCCAAAGCTT+TGG 0.318063 3_2:-21717665 Msa0399210:CDS
CCTGATGAAGCTGTGGCTTA+TGG 0.330474 3_2:-21717319 Msa0399210:CDS
AATGTTCTTGTCTTTGATCT+TGG 0.331932 3_2:-21717850 Msa0399210:CDS
AGTGAGAATTCCAAAGCTTT+GGG 0.334628 3_2:-21717664 Msa0399210:CDS
GAAATTGCTGAAACTTATTT+GGG 0.342870 3_2:-21718057 Msa0399210:CDS
ACACGGTTCCCTTGGTCGTT+TGG 0.354947 3_2:+21718357 None:intergenic
CGAAGGAATGTTCCAGGTTA+AGG 0.357597 3_2:-21717791 Msa0399210:CDS
GAGGGATGCCGAGCGGTATA+AGG 0.359508 3_2:-21716864 Msa0399210:CDS
TTCAGTAGTTGTTGAATCTT+CGG 0.360449 3_2:+21717394 None:intergenic
TTCCAGGTTAAGGCCACTTT+GGG 0.364020 3_2:-21717781 Msa0399210:CDS
ACCGATACTGAAAGATTAAT+AGG 0.373414 3_2:-21718312 Msa0399210:CDS
TTATGTCTTGTTGGACTGAT+TGG 0.374106 3_2:+21718205 None:intergenic
AGCTGCTATTGCTTATGGTT+TGG 0.376546 3_2:-21717902 Msa0399210:CDS
ATGGGTTTGTCTTTGTGGTT+TGG 0.379293 3_2:+21718159 None:intergenic
ACTCATCAATTTCTGCCAAT+TGG 0.383486 3_2:+21716711 None:intergenic
TCCAACAAGACATAAGGTTT+TGG 0.384795 3_2:-21718197 Msa0399210:CDS
AGGCAGAGGATGAGGAGGTT+AGG 0.400721 3_2:-21716844 Msa0399210:CDS
TCTTAACCTCCCCAAAGCTT+TGG 0.400779 3_2:+21717654 None:intergenic
ACTCAACCTTCCCTCCTTGT+TGG 0.402188 3_2:+21716910 None:intergenic
CTTGTCTTTGATCTTGGTGG+TGG 0.402810 3_2:-21717844 Msa0399210:CDS
GCCAAAACCTTATGTCTTGT+TGG 0.404961 3_2:+21718196 None:intergenic
GTTCATGAATTTGTTATTGT+CGG 0.409000 3_2:-21717433 Msa0399210:CDS
TTTGATGCCAAACGTTTGAT+TGG 0.412646 3_2:-21718243 Msa0399210:CDS
GTTCTTGTCTTTGATCTTGG+TGG 0.414303 3_2:-21717847 Msa0399210:CDS
TTTGATCAAAACTTTACCTT+GGG 0.431046 3_2:+21717120 None:intergenic
AGAATTATGTTTATGAAATG+AGG 0.432257 3_2:-21716793 Msa0399210:CDS
GTAGGGATCCTAGTATTCTT+CGG 0.433514 3_2:+21717169 None:intergenic
ATGAAATGAGGGAGAGAGTG+AGG 0.434187 3_2:-21716781 Msa0399210:CDS
TTTGTAATTGACAACAATCA+TGG 0.440479 3_2:+21718140 None:intergenic
CTCCCAAAGTGGCCTTAACC+TGG 0.442345 3_2:+21717779 None:intergenic
TGTTGACGAAGGAATGTTCC+AGG 0.444778 3_2:-21717797 Msa0399210:CDS
GTTCCAGGTTAAGGCCACTT+TGG 0.448544 3_2:-21717782 Msa0399210:CDS
TCGGTGAAGGCAACGTAAGA+AGG 0.448873 3_2:+21718330 None:intergenic
GGATGAGGAGGTTAGGAAGA+AGG 0.449706 3_2:-21716837 Msa0399210:CDS
GATTGAAGGCTTATTGCTGT+TGG 0.451601 3_2:-21717251 Msa0399210:CDS
ATGCTCTTTAGTACCTAGAA+AGG 0.452071 3_2:-21717032 Msa0399210:CDS
CCATAAGCCACAGCTTCATC+AGG 0.453102 3_2:+21717319 None:intergenic
ATCATTCTTCTCATCTCTTG+AGG 0.461303 3_2:+21716887 None:intergenic
AGTCTTGGTGTTGAAACTAA+AGG 0.463181 3_2:-21717214 Msa0399210:CDS
TATGAAATAATTCAACAAGA+TGG 0.465475 3_2:+21717713 None:intergenic
ACTGCTGAAGATAAGACAAA+AGG 0.472237 3_2:-21716956 Msa0399210:CDS
ACCTATTAATCTTTCAGTAT+CGG 0.473250 3_2:+21718311 None:intergenic
GCGGTATAAGGCAGAGGATG+AGG 0.474792 3_2:-21716852 Msa0399210:CDS
CTTTGATCAAAACTTTACCT+TGG 0.475667 3_2:+21717119 None:intergenic
GCGATGACCAATCAAACGTT+TGG 0.484985 3_2:+21718236 None:intergenic
AGGAGAACATGCTAAGACTA+AGG 0.487241 3_2:-21717089 Msa0399210:CDS
GAGGAAGCTTGAAAAGGTAG+TGG 0.489230 3_2:-21716762 Msa0399210:CDS
GAAGGAGACAAGAAGATTGA+AGG 0.489825 3_2:-21717265 Msa0399210:CDS
TGAGTTTGAATTCAAGAAGC+AGG 0.491801 3_2:-21716693 Msa0399210:CDS
TGAATTCAAGAAGCAGGAGT+TGG 0.497584 3_2:-21716687 Msa0399210:CDS
GATCCTCATCACATTAAAAC+CGG 0.502743 3_2:+21717942 None:intergenic
GAGAGTGAGGAAGCTTGAAA+AGG 0.502774 3_2:-21716768 Msa0399210:CDS
GAATTATGTTTATGAAATGA+GGG 0.504803 3_2:-21716792 Msa0399210:CDS
ATGGCTTGACAGAAACCAAT+TGG 0.505887 3_2:-21716726 Msa0399210:CDS
CTGCTGAAGATAAGACAAAA+GGG 0.507869 3_2:-21716955 Msa0399210:CDS
AATCAGTCCAACAAGACATA+AGG 0.516258 3_2:-21718203 Msa0399210:CDS
AAGGGGTGACACGGTTCCCT+TGG 0.517703 3_2:+21718349 None:intergenic
TGTACCAAGGTCAATGCCTA+TGG 0.520221 3_2:+21718428 None:intergenic
CATGAATTTGTTATTGTCGG+AGG 0.522698 3_2:-21717430 Msa0399210:CDS
TGTGAGTTGTTGAAGTAAGC+AGG 0.526143 3_2:+21717997 None:intergenic
TTGTAATTGACAACAATCAT+GGG 0.527199 3_2:+21718141 None:intergenic
CGGTGAAGGCAACGTAAGAA+GGG 0.529846 3_2:+21718331 None:intergenic
AGCTTGATTCACTATGTGAA+GGG 0.532350 3_2:-21717576 Msa0399210:CDS
CAAAACTTTACCTTGGGCTG+TGG 0.533300 3_2:+21717126 None:intergenic
TAGTGGAGGAAAGTATAGAA+TGG 0.539580 3_2:-21716745 Msa0399210:CDS
TCCTTCGTCAACAGTCACTA+AGG 0.546693 3_2:+21717807 None:intergenic
TGCCGAGCGGTATAAGGCAG+AGG 0.550389 3_2:-21716858 Msa0399210:CDS
ACCTCAAGAGATATCTTCCA+TGG 0.553297 3_2:-21718097 Msa0399210:CDS
ATGAATTTGTTATTGTCGGA+GGG 0.553906 3_2:-21717429 Msa0399210:CDS
ATTTCATAGAAAGTACAAGA+AGG 0.557642 3_2:-21717698 Msa0399210:CDS
ATCCTCTGCCTTATACCGCT+CGG 0.563812 3_2:+21716856 None:intergenic
GCAGCAATATTGAATACTGA+AGG 0.571285 3_2:-21717283 Msa0399210:CDS
GAAGATTCAACAACTACTGA+AGG 0.574421 3_2:-21717392 Msa0399210:CDS
GACAAGAAAATGTGGAGAGA+AGG 0.578701 3_2:-21717880 Msa0399210:CDS
ATGATTGTTGTCAATTACAA+AGG 0.584826 3_2:-21718138 Msa0399210:CDS
TAATCTTTCAGTATCGGTGA+AGG 0.586135 3_2:+21718317 None:intergenic
AGACAAGCTACAAAAGATGC+AGG 0.588448 3_2:-21717973 Msa0399210:CDS
ATCATTATCACCAACAAGGA+GGG 0.590966 3_2:-21716920 Msa0399210:CDS
AGAGATGAGAAGAATGATGA+GGG 0.595789 3_2:-21716882 Msa0399210:CDS
GAAGCTTGAAAAGGTAGTGG+AGG 0.595927 3_2:-21716759 Msa0399210:CDS
AAGGAAATGTTTAGTGTCAA+CGG 0.596200 3_2:-21717373 Msa0399210:CDS
TGTGGAGAAGTGTTTGATTG+AGG 0.604035 3_2:-21717476 Msa0399210:CDS
TACAGCTGTAGCTTGTACCA+AGG 0.607846 3_2:+21718415 None:intergenic
AGAATTCCAAAGCTTTGGGG+AGG 0.611200 3_2:-21717660 Msa0399210:CDS
CATTAATCCTGATGAAGCTG+TGG 0.613030 3_2:-21717326 Msa0399210:CDS
TAAGCAATAGCAGCTGCAGT+AGG 0.618882 3_2:+21717910 None:intergenic
ACTTTGGGAGATACTCATGT+GGG 0.620795 3_2:-21717766 Msa0399210:CDS
GTGAGAATTCCAAAGCTTTG+GGG 0.624343 3_2:-21717663 Msa0399210:CDS
AAGAGATGAGAAGAATGATG+AGG 0.628540 3_2:-21716883 Msa0399210:CDS
CAACGTAAGAAGGGGTGACA+CGG 0.629570 3_2:+21718340 None:intergenic
AGATCATTCCAAACGACCAA+GGG 0.630317 3_2:-21718365 Msa0399210:CDS
GAGATCATTCCAAACGACCA+AGG 0.630381 3_2:-21718366 Msa0399210:CDS
ATGGTTTGGACAAGAAAATG+TGG 0.630603 3_2:-21717888 Msa0399210:CDS
CACATGAGTATCTCCCAAAG+TGG 0.631307 3_2:+21717768 None:intergenic
TTATCACCAACAAGGAGGGA+AGG 0.634341 3_2:-21716916 Msa0399210:CDS
GAGCTTGATTCACTATGTGA+AGG 0.634799 3_2:-21717577 Msa0399210:CDS
CACTTTGGGAGATACTCATG+TGG 0.638927 3_2:-21717767 Msa0399210:CDS
AAAGCCATAGGCATTGACCT+TGG 0.638972 3_2:-21718432 Msa0399210:CDS
TCCTTAGTGACTGTTGACGA+AGG 0.640013 3_2:-21717808 Msa0399210:CDS
ACCATGGAAGATATCTCTTG+AGG 0.641800 3_2:+21718096 None:intergenic
GTATAAGGCAGAGGATGAGG+AGG 0.650304 3_2:-21716849 Msa0399210:CDS
CAGCATGCGAGAAAGCAAAG+AGG 0.658762 3_2:-21717630 Msa0399210:CDS
GATCATTATCACCAACAAGG+AGG 0.670278 3_2:-21716921 Msa0399210:CDS
GGTGAAGGCAACGTAAGAAG+GGG 0.671684 3_2:+21718332 None:intergenic
TTGGGAGATACTCATGTGGG+TGG 0.678875 3_2:-21717763 Msa0399210:CDS
CTTTAATTTAGACAACACCA+TGG 0.682894 3_2:+21718080 None:intergenic
CTTGGTGTTGAAACTAAAGG+TGG 0.707841 3_2:-21717211 Msa0399210:CDS
GAATGATGAGGGATGCCGAG+CGG 0.719182 3_2:-21716871 Msa0399210:CDS
GAAGATCATTATCACCAACA+AGG 0.723817 3_2:-21716924 Msa0399210:CDS
GCTACAGCTGTATTGCAGTG+TGG 0.732257 3_2:-21718404 Msa0399210:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCAAAACAAATATTGATATT+TGG + chr3_2:21718110-21718129 None:intergenic 15.0%
!! TCAAAACAAATATTGATATT+TGG + chr3_2:21718110-21718129 None:intergenic 15.0%
!!! TGTTGATTTTGATAACAATT+TGG - chr3_2:21717374-21717393 Msa0399210:CDS 20.0%
!! TATGAAATAATTCAACAAGA+TGG + chr3_2:21717404-21717423 None:intergenic 20.0%
!!! TTTTTTTGAGGAAATAAACA+AGG - chr3_2:21717590-21717609 Msa0399210:CDS 20.0%
!! AGAATTATGTTTATGAAATG+AGG - chr3_2:21718321-21718340 Msa0399210:CDS 20.0%
!! GAATTATGTTTATGAAATGA+GGG - chr3_2:21718322-21718341 Msa0399210:CDS 20.0%
!!! TGTTGATTTTGATAACAATT+TGG - chr3_2:21717374-21717393 Msa0399210:CDS 20.0%
!! TATGAAATAATTCAACAAGA+TGG + chr3_2:21717404-21717423 None:intergenic 20.0%
!!! TTTTTTTGAGGAAATAAACA+AGG - chr3_2:21717590-21717609 Msa0399210:CDS 20.0%
!! AGAATTATGTTTATGAAATG+AGG - chr3_2:21718321-21718340 Msa0399210:CDS 20.0%
!! GAATTATGTTTATGAAATGA+GGG - chr3_2:21718322-21718341 Msa0399210:CDS 20.0%
! ACCTATTAATCTTTCAGTAT+CGG + chr3_2:21716806-21716825 None:intergenic 25.0%
! TTGTAATTGACAACAATCAT+GGG + chr3_2:21716976-21716995 None:intergenic 25.0%
! TTTGTAATTGACAACAATCA+TGG + chr3_2:21716977-21716996 None:intergenic 25.0%
! ATGATTGTTGTCAATTACAA+AGG - chr3_2:21716976-21716995 Msa0399210:CDS 25.0%
! GAAATTGCTGAAACTTATTT+GGG - chr3_2:21717057-21717076 Msa0399210:CDS 25.0%
!! ATTTCATAGAAAGTACAAGA+AGG - chr3_2:21717416-21717435 Msa0399210:CDS 25.0%
!!! GTTCATGAATTTGTTATTGT+CGG - chr3_2:21717681-21717700 Msa0399210:CDS 25.0%
!!! AAACATTCTCCTTTTTTGTA+GGG + chr3_2:21717965-21717984 None:intergenic 25.0%
!!! AAAACATTCTCCTTTTTTGT+AGG + chr3_2:21717966-21717985 None:intergenic 25.0%
! TTTGATCAAAACTTTACCTT+GGG + chr3_2:21717997-21718016 None:intergenic 25.0%
!!! TGTTTTGATGTTGATGTTAA+TGG - chr3_2:21718122-21718141 Msa0399210:CDS 25.0%
!! TGTTGATGTTAATGGAATTT+TGG - chr3_2:21718130-21718149 Msa0399210:CDS 25.0%
! ACCTATTAATCTTTCAGTAT+CGG + chr3_2:21716806-21716825 None:intergenic 25.0%
! TTGTAATTGACAACAATCAT+GGG + chr3_2:21716976-21716995 None:intergenic 25.0%
! TTTGTAATTGACAACAATCA+TGG + chr3_2:21716977-21716996 None:intergenic 25.0%
! ATGATTGTTGTCAATTACAA+AGG - chr3_2:21716976-21716995 Msa0399210:CDS 25.0%
! GAAATTGCTGAAACTTATTT+GGG - chr3_2:21717057-21717076 Msa0399210:CDS 25.0%
!! ATTTCATAGAAAGTACAAGA+AGG - chr3_2:21717416-21717435 Msa0399210:CDS 25.0%
!!! GTTCATGAATTTGTTATTGT+CGG - chr3_2:21717681-21717700 Msa0399210:CDS 25.0%
!!! AAACATTCTCCTTTTTTGTA+GGG + chr3_2:21717965-21717984 None:intergenic 25.0%
!!! AAAACATTCTCCTTTTTTGT+AGG + chr3_2:21717966-21717985 None:intergenic 25.0%
! TTTGATCAAAACTTTACCTT+GGG + chr3_2:21717997-21718016 None:intergenic 25.0%
!!! TGTTTTGATGTTGATGTTAA+TGG - chr3_2:21718122-21718141 Msa0399210:CDS 25.0%
!! TGTTGATGTTAATGGAATTT+TGG - chr3_2:21718130-21718149 Msa0399210:CDS 25.0%
ACCGATACTGAAAGATTAAT+AGG - chr3_2:21716802-21716821 Msa0399210:CDS 30.0%
!!! ATTTTTGGCTGATTGGTTTA+TGG + chr3_2:21716836-21716855 None:intergenic 30.0%
! GAGGTGAAAAATGTTTTTCT+TGG + chr3_2:21717002-21717021 None:intergenic 30.0%
! CTTTAATTTAGACAACACCA+TGG + chr3_2:21717037-21717056 None:intergenic 30.0%
!! CATGGTGTTGTCTAAATTAA+AGG - chr3_2:21717035-21717054 Msa0399210:CDS 30.0%
GGAAATTGCTGAAACTTATT+TGG - chr3_2:21717056-21717075 Msa0399210:CDS 30.0%
AATGTTCTTGTCTTTGATCT+TGG - chr3_2:21717264-21717283 Msa0399210:CDS 30.0%
!!! TGTTACTAGAGCTTTTTTTG+AGG - chr3_2:21717578-21717597 Msa0399210:CDS 30.0%
!! ATGAATTTGTTATTGTCGGA+GGG - chr3_2:21717685-21717704 Msa0399210:CDS 30.0%
TTCAGTAGTTGTTGAATCTT+CGG + chr3_2:21717723-21717742 None:intergenic 30.0%
AAGGAAATGTTTAGTGTCAA+CGG - chr3_2:21717741-21717760 Msa0399210:CDS 30.0%
!! TTTTGATTCCGAAGAATACT+AGG - chr3_2:21717937-21717956 Msa0399210:CDS 30.0%
CTTTGATCAAAACTTTACCT+TGG + chr3_2:21717998-21718017 None:intergenic 30.0%
!! GTTTTGATCAAAGTCTATGA+AGG - chr3_2:21718005-21718024 Msa0399210:CDS 30.0%
! TTGATATTTGGAACCTTTCT+AGG + chr3_2:21718098-21718117 None:intergenic 30.0%
! TGATGTTAATGGAATTTTGG+AGG - chr3_2:21718133-21718152 Msa0399210:CDS 30.0%
ACCGATACTGAAAGATTAAT+AGG - chr3_2:21716802-21716821 Msa0399210:CDS 30.0%
!!! ATTTTTGGCTGATTGGTTTA+TGG + chr3_2:21716836-21716855 None:intergenic 30.0%
! GAGGTGAAAAATGTTTTTCT+TGG + chr3_2:21717002-21717021 None:intergenic 30.0%
! CTTTAATTTAGACAACACCA+TGG + chr3_2:21717037-21717056 None:intergenic 30.0%
!! CATGGTGTTGTCTAAATTAA+AGG - chr3_2:21717035-21717054 Msa0399210:CDS 30.0%
GGAAATTGCTGAAACTTATT+TGG - chr3_2:21717056-21717075 Msa0399210:CDS 30.0%
AATGTTCTTGTCTTTGATCT+TGG - chr3_2:21717264-21717283 Msa0399210:CDS 30.0%
!!! TGTTACTAGAGCTTTTTTTG+AGG - chr3_2:21717578-21717597 Msa0399210:CDS 30.0%
!! ATGAATTTGTTATTGTCGGA+GGG - chr3_2:21717685-21717704 Msa0399210:CDS 30.0%
TTCAGTAGTTGTTGAATCTT+CGG + chr3_2:21717723-21717742 None:intergenic 30.0%
AAGGAAATGTTTAGTGTCAA+CGG - chr3_2:21717741-21717760 Msa0399210:CDS 30.0%
!! TTTTGATTCCGAAGAATACT+AGG - chr3_2:21717937-21717956 Msa0399210:CDS 30.0%
CTTTGATCAAAACTTTACCT+TGG + chr3_2:21717998-21718017 None:intergenic 30.0%
!! GTTTTGATCAAAGTCTATGA+AGG - chr3_2:21718005-21718024 Msa0399210:CDS 30.0%
! TTGATATTTGGAACCTTTCT+AGG + chr3_2:21718098-21718117 None:intergenic 30.0%
! TGATGTTAATGGAATTTTGG+AGG - chr3_2:21718133-21718152 Msa0399210:CDS 30.0%
ACAAGAAAAAGCAAAGCCAT+AGG - chr3_2:21716670-21716689 Msa0399210:CDS 35.0%
!! TAATCTTTCAGTATCGGTGA+AGG + chr3_2:21716800-21716819 None:intergenic 35.0%
CAAACGTTTGGCATCAAAAA+CGG + chr3_2:21716869-21716888 None:intergenic 35.0%
TTTGATGCCAAACGTTTGAT+TGG - chr3_2:21716871-21716890 Msa0399210:CDS 35.0%
! TTATGTCTTGTTGGACTGAT+TGG + chr3_2:21716912-21716931 None:intergenic 35.0%
AATCAGTCCAACAAGACATA+AGG - chr3_2:21716911-21716930 Msa0399210:CDS 35.0%
! TCCAACAAGACATAAGGTTT+TGG - chr3_2:21716917-21716936 Msa0399210:CDS 35.0%
!! CATAAGGTTTTGGCCTTTTA+AGG - chr3_2:21716927-21716946 Msa0399210:CDS 35.0%
! TGGTTTGGAACAACCTTAAA+AGG + chr3_2:21716943-21716962 None:intergenic 35.0%
AAAGATGCAGGAAAAATTGC+CGG - chr3_2:21717153-21717172 Msa0399210:CDS 35.0%
GATCCTCATCACATTAAAAC+CGG + chr3_2:21717175-21717194 None:intergenic 35.0%
! ATGGTTTGGACAAGAAAATG+TGG - chr3_2:21717226-21717245 Msa0399210:CDS 35.0%
AGTGAGAATTCCAAAGCTTT+GGG - chr3_2:21717450-21717469 Msa0399210:CDS 35.0%
AGCTTGATTCACTATGTGAA+GGG - chr3_2:21717538-21717557 Msa0399210:CDS 35.0%
!! CATGAATTTGTTATTGTCGG+AGG - chr3_2:21717684-21717703 Msa0399210:CDS 35.0%
GAAGATTCAACAACTACTGA+AGG - chr3_2:21717722-21717741 Msa0399210:CDS 35.0%
! TCAGGATTAATGCTTTTGCA+AGG + chr3_2:21717780-21717799 None:intergenic 35.0%
GCAGCAATATTGAATACTGA+AGG - chr3_2:21717831-21717850 Msa0399210:CDS 35.0%
! GATGTTATGCCTTTTAGTCT+TGG - chr3_2:21717885-21717904 Msa0399210:CDS 35.0%
GTTTCAACACCAAGACTAAA+AGG + chr3_2:21717897-21717916 None:intergenic 35.0%
!! AGTCTTGGTGTTGAAACTAA+AGG - chr3_2:21717900-21717919 Msa0399210:CDS 35.0%
TACTAGGATCCCTACAAAAA+AGG - chr3_2:21717953-21717972 Msa0399210:CDS 35.0%
! ACTAAGGACAACTTTTTGCT+TGG - chr3_2:21718041-21718060 Msa0399210:CDS 35.0%
ATGCTCTTTAGTACCTAGAA+AGG - chr3_2:21718082-21718101 Msa0399210:CDS 35.0%
ACTGCTGAAGATAAGACAAA+AGG - chr3_2:21718158-21718177 Msa0399210:CDS 35.0%
CTGCTGAAGATAAGACAAAA+GGG - chr3_2:21718159-21718178 Msa0399210:CDS 35.0%
GAAGATCATTATCACCAACA+AGG - chr3_2:21718190-21718209 Msa0399210:CDS 35.0%
ATCATTATCACCAACAAGGA+GGG - chr3_2:21718194-21718213 Msa0399210:CDS 35.0%
ATCATTCTTCTCATCTCTTG+AGG + chr3_2:21718230-21718249 None:intergenic 35.0%
AAGAGATGAGAAGAATGATG+AGG - chr3_2:21718231-21718250 Msa0399210:CDS 35.0%
AGAGATGAGAAGAATGATGA+GGG - chr3_2:21718232-21718251 Msa0399210:CDS 35.0%
! TAGTGGAGGAAAGTATAGAA+TGG - chr3_2:21718369-21718388 Msa0399210:CDS 35.0%
ACTCATCAATTTCTGCCAAT+TGG + chr3_2:21718406-21718425 None:intergenic 35.0%
TGAGTTTGAATTCAAGAAGC+AGG - chr3_2:21718421-21718440 Msa0399210:CDS 35.0%
ACAAGAAAAAGCAAAGCCAT+AGG - chr3_2:21716670-21716689 Msa0399210:CDS 35.0%
!! TAATCTTTCAGTATCGGTGA+AGG + chr3_2:21716800-21716819 None:intergenic 35.0%
CAAACGTTTGGCATCAAAAA+CGG + chr3_2:21716869-21716888 None:intergenic 35.0%
TTTGATGCCAAACGTTTGAT+TGG - chr3_2:21716871-21716890 Msa0399210:CDS 35.0%
! TTATGTCTTGTTGGACTGAT+TGG + chr3_2:21716912-21716931 None:intergenic 35.0%
AATCAGTCCAACAAGACATA+AGG - chr3_2:21716911-21716930 Msa0399210:CDS 35.0%
! TCCAACAAGACATAAGGTTT+TGG - chr3_2:21716917-21716936 Msa0399210:CDS 35.0%
!! CATAAGGTTTTGGCCTTTTA+AGG - chr3_2:21716927-21716946 Msa0399210:CDS 35.0%
! TGGTTTGGAACAACCTTAAA+AGG + chr3_2:21716943-21716962 None:intergenic 35.0%
AAAGATGCAGGAAAAATTGC+CGG - chr3_2:21717153-21717172 Msa0399210:CDS 35.0%
GATCCTCATCACATTAAAAC+CGG + chr3_2:21717175-21717194 None:intergenic 35.0%
! ATGGTTTGGACAAGAAAATG+TGG - chr3_2:21717226-21717245 Msa0399210:CDS 35.0%
AGTGAGAATTCCAAAGCTTT+GGG - chr3_2:21717450-21717469 Msa0399210:CDS 35.0%
AGCTTGATTCACTATGTGAA+GGG - chr3_2:21717538-21717557 Msa0399210:CDS 35.0%
!! CATGAATTTGTTATTGTCGG+AGG - chr3_2:21717684-21717703 Msa0399210:CDS 35.0%
GAAGATTCAACAACTACTGA+AGG - chr3_2:21717722-21717741 Msa0399210:CDS 35.0%
! TCAGGATTAATGCTTTTGCA+AGG + chr3_2:21717780-21717799 None:intergenic 35.0%
GCAGCAATATTGAATACTGA+AGG - chr3_2:21717831-21717850 Msa0399210:CDS 35.0%
! GATGTTATGCCTTTTAGTCT+TGG - chr3_2:21717885-21717904 Msa0399210:CDS 35.0%
GTTTCAACACCAAGACTAAA+AGG + chr3_2:21717897-21717916 None:intergenic 35.0%
!! AGTCTTGGTGTTGAAACTAA+AGG - chr3_2:21717900-21717919 Msa0399210:CDS 35.0%
TACTAGGATCCCTACAAAAA+AGG - chr3_2:21717953-21717972 Msa0399210:CDS 35.0%
! ACTAAGGACAACTTTTTGCT+TGG - chr3_2:21718041-21718060 Msa0399210:CDS 35.0%
ATGCTCTTTAGTACCTAGAA+AGG - chr3_2:21718082-21718101 Msa0399210:CDS 35.0%
ACTGCTGAAGATAAGACAAA+AGG - chr3_2:21718158-21718177 Msa0399210:CDS 35.0%
CTGCTGAAGATAAGACAAAA+GGG - chr3_2:21718159-21718178 Msa0399210:CDS 35.0%
GAAGATCATTATCACCAACA+AGG - chr3_2:21718190-21718209 Msa0399210:CDS 35.0%
ATCATTATCACCAACAAGGA+GGG - chr3_2:21718194-21718213 Msa0399210:CDS 35.0%
ATCATTCTTCTCATCTCTTG+AGG + chr3_2:21718230-21718249 None:intergenic 35.0%
AAGAGATGAGAAGAATGATG+AGG - chr3_2:21718231-21718250 Msa0399210:CDS 35.0%
AGAGATGAGAAGAATGATGA+GGG - chr3_2:21718232-21718251 Msa0399210:CDS 35.0%
! TAGTGGAGGAAAGTATAGAA+TGG - chr3_2:21718369-21718388 Msa0399210:CDS 35.0%
ACTCATCAATTTCTGCCAAT+TGG + chr3_2:21718406-21718425 None:intergenic 35.0%
TGAGTTTGAATTCAAGAAGC+AGG - chr3_2:21718421-21718440 Msa0399210:CDS 35.0%
AGATCATTCCAAACGACCAA+GGG - chr3_2:21716749-21716768 Msa0399210:CDS 40.0%
!!! TGTGTGGATTTTTGGCTGAT+TGG + chr3_2:21716843-21716862 None:intergenic 40.0%
!!! AACGGTGTTGTGTGGATTTT+TGG + chr3_2:21716851-21716870 None:intergenic 40.0%
GCCAAAACCTTATGTCTTGT+TGG + chr3_2:21716921-21716940 None:intergenic 40.0%
ATGGGTTTGTCTTTGTGGTT+TGG + chr3_2:21716958-21716977 None:intergenic 40.0%
CAATCATGGGTTTGTCTTTG+TGG + chr3_2:21716963-21716982 None:intergenic 40.0%
ACCTCAAGAGATATCTTCCA+TGG - chr3_2:21717017-21717036 Msa0399210:CDS 40.0%
ACCATGGAAGATATCTCTTG+AGG + chr3_2:21717021-21717040 None:intergenic 40.0%
TGTGAGTTGTTGAAGTAAGC+AGG + chr3_2:21717120-21717139 None:intergenic 40.0%
AGACAAGCTACAAAAGATGC+AGG - chr3_2:21717141-21717160 Msa0399210:CDS 40.0%
!!! TTGCCGGTTTTAATGTGATG+AGG - chr3_2:21717169-21717188 Msa0399210:CDS 40.0%
AGCTGCTATTGCTTATGGTT+TGG - chr3_2:21717212-21717231 Msa0399210:CDS 40.0%
GACAAGAAAATGTGGAGAGA+AGG - chr3_2:21717234-21717253 Msa0399210:CDS 40.0%
GTTCTTGTCTTTGATCTTGG+TGG - chr3_2:21717267-21717286 Msa0399210:CDS 40.0%
! ACTTTGGGAGATACTCATGT+GGG - chr3_2:21717348-21717367 Msa0399210:CDS 40.0%
CAGTGAGAATTCCAAAGCTT+TGG - chr3_2:21717449-21717468 Msa0399210:CDS 40.0%
GTGAGAATTCCAAAGCTTTG+GGG - chr3_2:21717451-21717470 Msa0399210:CDS 40.0%
GAGCTTGATTCACTATGTGA+AGG - chr3_2:21717537-21717556 Msa0399210:CDS 40.0%
! CAAAAAGTGCATGAAGACTG+TGG - chr3_2:21717620-21717639 Msa0399210:CDS 40.0%
!! TGTGGAGAAGTGTTTGATTG+AGG - chr3_2:21717638-21717657 Msa0399210:CDS 40.0%
CATTAATCCTGATGAAGCTG+TGG - chr3_2:21717788-21717807 Msa0399210:CDS 40.0%
GAAGGAGACAAGAAGATTGA+AGG - chr3_2:21717849-21717868 Msa0399210:CDS 40.0%
GATTGAAGGCTTATTGCTGT+TGG - chr3_2:21717863-21717882 Msa0399210:CDS 40.0%
!! CTTGGTGTTGAAACTAAAGG+TGG - chr3_2:21717903-21717922 Msa0399210:CDS 40.0%
GTAGGGATCCTAGTATTCTT+CGG + chr3_2:21717948-21717967 None:intergenic 40.0%
AGGAGAACATGCTAAGACTA+AGG - chr3_2:21718025-21718044 Msa0399210:CDS 40.0%
GATCATTATCACCAACAAGG+AGG - chr3_2:21718193-21718212 Msa0399210:CDS 40.0%
ATGGCTTGACAGAAACCAAT+TGG - chr3_2:21718388-21718407 Msa0399210:CDS 40.0%
TGAATTCAAGAAGCAGGAGT+TGG - chr3_2:21718427-21718446 Msa0399210:CDS 40.0%
AGATCATTCCAAACGACCAA+GGG - chr3_2:21716749-21716768 Msa0399210:CDS 40.0%
!!! TGTGTGGATTTTTGGCTGAT+TGG + chr3_2:21716843-21716862 None:intergenic 40.0%
!!! AACGGTGTTGTGTGGATTTT+TGG + chr3_2:21716851-21716870 None:intergenic 40.0%
GCCAAAACCTTATGTCTTGT+TGG + chr3_2:21716921-21716940 None:intergenic 40.0%
ATGGGTTTGTCTTTGTGGTT+TGG + chr3_2:21716958-21716977 None:intergenic 40.0%
CAATCATGGGTTTGTCTTTG+TGG + chr3_2:21716963-21716982 None:intergenic 40.0%
ACCTCAAGAGATATCTTCCA+TGG - chr3_2:21717017-21717036 Msa0399210:CDS 40.0%
ACCATGGAAGATATCTCTTG+AGG + chr3_2:21717021-21717040 None:intergenic 40.0%
TGTGAGTTGTTGAAGTAAGC+AGG + chr3_2:21717120-21717139 None:intergenic 40.0%
AGACAAGCTACAAAAGATGC+AGG - chr3_2:21717141-21717160 Msa0399210:CDS 40.0%
!!! TTGCCGGTTTTAATGTGATG+AGG - chr3_2:21717169-21717188 Msa0399210:CDS 40.0%
AGCTGCTATTGCTTATGGTT+TGG - chr3_2:21717212-21717231 Msa0399210:CDS 40.0%
GACAAGAAAATGTGGAGAGA+AGG - chr3_2:21717234-21717253 Msa0399210:CDS 40.0%
GTTCTTGTCTTTGATCTTGG+TGG - chr3_2:21717267-21717286 Msa0399210:CDS 40.0%
! ACTTTGGGAGATACTCATGT+GGG - chr3_2:21717348-21717367 Msa0399210:CDS 40.0%
CAGTGAGAATTCCAAAGCTT+TGG - chr3_2:21717449-21717468 Msa0399210:CDS 40.0%
GTGAGAATTCCAAAGCTTTG+GGG - chr3_2:21717451-21717470 Msa0399210:CDS 40.0%
GAGCTTGATTCACTATGTGA+AGG - chr3_2:21717537-21717556 Msa0399210:CDS 40.0%
! CAAAAAGTGCATGAAGACTG+TGG - chr3_2:21717620-21717639 Msa0399210:CDS 40.0%
!! TGTGGAGAAGTGTTTGATTG+AGG - chr3_2:21717638-21717657 Msa0399210:CDS 40.0%
CATTAATCCTGATGAAGCTG+TGG - chr3_2:21717788-21717807 Msa0399210:CDS 40.0%
GAAGGAGACAAGAAGATTGA+AGG - chr3_2:21717849-21717868 Msa0399210:CDS 40.0%
GATTGAAGGCTTATTGCTGT+TGG - chr3_2:21717863-21717882 Msa0399210:CDS 40.0%
!! CTTGGTGTTGAAACTAAAGG+TGG - chr3_2:21717903-21717922 Msa0399210:CDS 40.0%
GTAGGGATCCTAGTATTCTT+CGG + chr3_2:21717948-21717967 None:intergenic 40.0%
AGGAGAACATGCTAAGACTA+AGG - chr3_2:21718025-21718044 Msa0399210:CDS 40.0%
GATCATTATCACCAACAAGG+AGG - chr3_2:21718193-21718212 Msa0399210:CDS 40.0%
ATGGCTTGACAGAAACCAAT+TGG - chr3_2:21718388-21718407 Msa0399210:CDS 40.0%
TGAATTCAAGAAGCAGGAGT+TGG - chr3_2:21718427-21718446 Msa0399210:CDS 40.0%
!! AAAGCCATAGGCATTGACCT+TGG - chr3_2:21716682-21716701 Msa0399210:CDS 45.0%
TGTACCAAGGTCAATGCCTA+TGG + chr3_2:21716689-21716708 None:intergenic 45.0%
! TACAGCTGTAGCTTGTACCA+AGG + chr3_2:21716702-21716721 None:intergenic 45.0%
GAGATCATTCCAAACGACCA+AGG - chr3_2:21716748-21716767 Msa0399210:CDS 45.0%
!! GCATCAAAAACGGTGTTGTG+TGG + chr3_2:21716859-21716878 None:intergenic 45.0%
GCGATGACCAATCAAACGTT+TGG + chr3_2:21716881-21716900 None:intergenic 45.0%
TAAGCAATAGCAGCTGCAGT+AGG + chr3_2:21717207-21717226 None:intergenic 45.0%
ACTGCAGCTGCTATTGCTTA+TGG - chr3_2:21717207-21717226 Msa0399210:CDS 45.0%
CTTGTCTTTGATCTTGGTGG+TGG - chr3_2:21717270-21717289 Msa0399210:CDS 45.0%
TCCTTAGTGACTGTTGACGA+AGG - chr3_2:21717306-21717325 Msa0399210:CDS 45.0%
TCCTTCGTCAACAGTCACTA+AGG + chr3_2:21717310-21717329 None:intergenic 45.0%
TGTTGACGAAGGAATGTTCC+AGG - chr3_2:21717317-21717336 Msa0399210:CDS 45.0%
CGAAGGAATGTTCCAGGTTA+AGG - chr3_2:21717323-21717342 Msa0399210:CDS 45.0%
! TTCCAGGTTAAGGCCACTTT+GGG - chr3_2:21717333-21717352 Msa0399210:CDS 45.0%
CACATGAGTATCTCCCAAAG+TGG + chr3_2:21717349-21717368 None:intergenic 45.0%
! CACTTTGGGAGATACTCATG+TGG - chr3_2:21717347-21717366 Msa0399210:CDS 45.0%
!! AGAATTCCAAAGCTTTGGGG+AGG - chr3_2:21717454-21717473 Msa0399210:CDS 45.0%
TCTTAACCTCCCCAAAGCTT+TGG + chr3_2:21717463-21717482 None:intergenic 45.0%
! AATGTTTTCTCCACAGCCCA+AGG - chr3_2:21717978-21717997 Msa0399210:CDS 45.0%
CAAAACTTTACCTTGGGCTG+TGG + chr3_2:21717991-21718010 None:intergenic 45.0%
TTATCACCAACAAGGAGGGA+AGG - chr3_2:21718198-21718217 Msa0399210:CDS 45.0%
ATGAAATGAGGGAGAGAGTG+AGG - chr3_2:21718333-21718352 Msa0399210:CDS 45.0%
GAGAGTGAGGAAGCTTGAAA+AGG - chr3_2:21718346-21718365 Msa0399210:CDS 45.0%
GAGGAAGCTTGAAAAGGTAG+TGG - chr3_2:21718352-21718371 Msa0399210:CDS 45.0%
! GAAGCTTGAAAAGGTAGTGG+AGG - chr3_2:21718355-21718374 Msa0399210:CDS 45.0%
!! AAAGCCATAGGCATTGACCT+TGG - chr3_2:21716682-21716701 Msa0399210:CDS 45.0%
TGTACCAAGGTCAATGCCTA+TGG + chr3_2:21716689-21716708 None:intergenic 45.0%
! TACAGCTGTAGCTTGTACCA+AGG + chr3_2:21716702-21716721 None:intergenic 45.0%
GAGATCATTCCAAACGACCA+AGG - chr3_2:21716748-21716767 Msa0399210:CDS 45.0%
!! GCATCAAAAACGGTGTTGTG+TGG + chr3_2:21716859-21716878 None:intergenic 45.0%
GCGATGACCAATCAAACGTT+TGG + chr3_2:21716881-21716900 None:intergenic 45.0%
TAAGCAATAGCAGCTGCAGT+AGG + chr3_2:21717207-21717226 None:intergenic 45.0%
ACTGCAGCTGCTATTGCTTA+TGG - chr3_2:21717207-21717226 Msa0399210:CDS 45.0%
CTTGTCTTTGATCTTGGTGG+TGG - chr3_2:21717270-21717289 Msa0399210:CDS 45.0%
TCCTTAGTGACTGTTGACGA+AGG - chr3_2:21717306-21717325 Msa0399210:CDS 45.0%
TCCTTCGTCAACAGTCACTA+AGG + chr3_2:21717310-21717329 None:intergenic 45.0%
TGTTGACGAAGGAATGTTCC+AGG - chr3_2:21717317-21717336 Msa0399210:CDS 45.0%
CGAAGGAATGTTCCAGGTTA+AGG - chr3_2:21717323-21717342 Msa0399210:CDS 45.0%
! TTCCAGGTTAAGGCCACTTT+GGG - chr3_2:21717333-21717352 Msa0399210:CDS 45.0%
CACATGAGTATCTCCCAAAG+TGG + chr3_2:21717349-21717368 None:intergenic 45.0%
! CACTTTGGGAGATACTCATG+TGG - chr3_2:21717347-21717366 Msa0399210:CDS 45.0%
!! AGAATTCCAAAGCTTTGGGG+AGG - chr3_2:21717454-21717473 Msa0399210:CDS 45.0%
TCTTAACCTCCCCAAAGCTT+TGG + chr3_2:21717463-21717482 None:intergenic 45.0%
! AATGTTTTCTCCACAGCCCA+AGG - chr3_2:21717978-21717997 Msa0399210:CDS 45.0%
CAAAACTTTACCTTGGGCTG+TGG + chr3_2:21717991-21718010 None:intergenic 45.0%
TTATCACCAACAAGGAGGGA+AGG - chr3_2:21718198-21718217 Msa0399210:CDS 45.0%
ATGAAATGAGGGAGAGAGTG+AGG - chr3_2:21718333-21718352 Msa0399210:CDS 45.0%
GAGAGTGAGGAAGCTTGAAA+AGG - chr3_2:21718346-21718365 Msa0399210:CDS 45.0%
GAGGAAGCTTGAAAAGGTAG+TGG - chr3_2:21718352-21718371 Msa0399210:CDS 45.0%
! GAAGCTTGAAAAGGTAGTGG+AGG - chr3_2:21718355-21718374 Msa0399210:CDS 45.0%
GCTACAGCTGTATTGCAGTG+TGG - chr3_2:21716710-21716729 Msa0399210:CDS 50.0%
CAACGTAAGAAGGGGTGACA+CGG + chr3_2:21716777-21716796 None:intergenic 50.0%
GGTGAAGGCAACGTAAGAAG+GGG + chr3_2:21716785-21716804 None:intergenic 50.0%
CGGTGAAGGCAACGTAAGAA+GGG + chr3_2:21716786-21716805 None:intergenic 50.0%
TCGGTGAAGGCAACGTAAGA+AGG + chr3_2:21716787-21716806 None:intergenic 50.0%
GTTCCAGGTTAAGGCCACTT+TGG - chr3_2:21717332-21717351 Msa0399210:CDS 50.0%
TTGGGAGATACTCATGTGGG+TGG - chr3_2:21717351-21717370 Msa0399210:CDS 50.0%
CAGCATGCGAGAAAGCAAAG+AGG - chr3_2:21717484-21717503 Msa0399210:CDS 50.0%
CCATAAGCCACAGCTTCATC+AGG + chr3_2:21717798-21717817 None:intergenic 50.0%
CCTGATGAAGCTGTGGCTTA+TGG - chr3_2:21717795-21717814 Msa0399210:CDS 50.0%
ACTCAACCTTCCCTCCTTGT+TGG + chr3_2:21718207-21718226 None:intergenic 50.0%
ATCCTCTGCCTTATACCGCT+CGG + chr3_2:21718261-21718280 None:intergenic 50.0%
GTATAAGGCAGAGGATGAGG+AGG - chr3_2:21718265-21718284 Msa0399210:CDS 50.0%
GGATGAGGAGGTTAGGAAGA+AGG - chr3_2:21718277-21718296 Msa0399210:CDS 50.0%
GCTACAGCTGTATTGCAGTG+TGG - chr3_2:21716710-21716729 Msa0399210:CDS 50.0%
CAACGTAAGAAGGGGTGACA+CGG + chr3_2:21716777-21716796 None:intergenic 50.0%
GGTGAAGGCAACGTAAGAAG+GGG + chr3_2:21716785-21716804 None:intergenic 50.0%
CGGTGAAGGCAACGTAAGAA+GGG + chr3_2:21716786-21716805 None:intergenic 50.0%
TCGGTGAAGGCAACGTAAGA+AGG + chr3_2:21716787-21716806 None:intergenic 50.0%
GTTCCAGGTTAAGGCCACTT+TGG - chr3_2:21717332-21717351 Msa0399210:CDS 50.0%
TTGGGAGATACTCATGTGGG+TGG - chr3_2:21717351-21717370 Msa0399210:CDS 50.0%
CAGCATGCGAGAAAGCAAAG+AGG - chr3_2:21717484-21717503 Msa0399210:CDS 50.0%
CCATAAGCCACAGCTTCATC+AGG + chr3_2:21717798-21717817 None:intergenic 50.0%
CCTGATGAAGCTGTGGCTTA+TGG - chr3_2:21717795-21717814 Msa0399210:CDS 50.0%
ACTCAACCTTCCCTCCTTGT+TGG + chr3_2:21718207-21718226 None:intergenic 50.0%
ATCCTCTGCCTTATACCGCT+CGG + chr3_2:21718261-21718280 None:intergenic 50.0%
GTATAAGGCAGAGGATGAGG+AGG - chr3_2:21718265-21718284 Msa0399210:CDS 50.0%
GGATGAGGAGGTTAGGAAGA+AGG - chr3_2:21718277-21718296 Msa0399210:CDS 50.0%
ACACGGTTCCCTTGGTCGTT+TGG + chr3_2:21716760-21716779 None:intergenic 55.0%
! CTCCCAAAGTGGCCTTAACC+TGG + chr3_2:21717338-21717357 None:intergenic 55.0%
GAATGATGAGGGATGCCGAG+CGG - chr3_2:21718243-21718262 Msa0399210:CDS 55.0%
GCGGTATAAGGCAGAGGATG+AGG - chr3_2:21718262-21718281 Msa0399210:CDS 55.0%
AGGCAGAGGATGAGGAGGTT+AGG - chr3_2:21718270-21718289 Msa0399210:CDS 55.0%
ACACGGTTCCCTTGGTCGTT+TGG + chr3_2:21716760-21716779 None:intergenic 55.0%
! CTCCCAAAGTGGCCTTAACC+TGG + chr3_2:21717338-21717357 None:intergenic 55.0%
GAATGATGAGGGATGCCGAG+CGG - chr3_2:21718243-21718262 Msa0399210:CDS 55.0%
GCGGTATAAGGCAGAGGATG+AGG - chr3_2:21718262-21718281 Msa0399210:CDS 55.0%
AGGCAGAGGATGAGGAGGTT+AGG - chr3_2:21718270-21718289 Msa0399210:CDS 55.0%
AAGGGGTGACACGGTTCCCT+TGG + chr3_2:21716768-21716787 None:intergenic 60.0%
GAGGGATGCCGAGCGGTATA+AGG - chr3_2:21718250-21718269 Msa0399210:CDS 60.0%
TGCCGAGCGGTATAAGGCAG+AGG - chr3_2:21718256-21718275 Msa0399210:CDS 60.0%
AAGGGGTGACACGGTTCCCT+TGG + chr3_2:21716768-21716787 None:intergenic 60.0%
GAGGGATGCCGAGCGGTATA+AGG - chr3_2:21718250-21718269 Msa0399210:CDS 60.0%
TGCCGAGCGGTATAAGGCAG+AGG - chr3_2:21718256-21718275 Msa0399210:CDS 60.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 21716664 21718472 21716664 ID=Msa0399210;Name=Msa0399210
chr3_2 mRNA 21716664 21718472 21716664 ID=Msa0399210-mRNA-1;Parent=Msa0399210;Name=Msa0399210-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|602
chr3_2 exon 21716664 21718472 21716664 ID=Msa0399210-mRNA-1:exon:4714;Parent=Msa0399210-mRNA-1
chr3_2 CDS 21716664 21718472 21716664 ID=Msa0399210-mRNA-1:cds;Parent=Msa0399210-mRNA-1
Gene Sequence

>Msa0399210

ATGGCAACAAGAAAAAGCAAAGCCATAGGCATTGACCTTGGTACAAGCTACAGCTGTATTGCAGTGTGGCGAAACAATTGTGTTGAGATCATTCCAAACGACCAAGGGAACCGTGTCACCCCTTCTTACGTTGCCTTCACCGATACTGAAAGATTAATAGGTGATGCTGCCATAAACCAATCAGCCAAAAATCCACACAACACCGTTTTTGATGCCAAACGTTTGATTGGTCATCGCTTCTCTGACCAATCAGTCCAACAAGACATAAGGTTTTGGCCTTTTAAGGTTGTTCCAAACCACAAAGACAAACCCATGATTGTTGTCAATTACAAAGGCCAAGAAAAACATTTTTCACCTCAAGAGATATCTTCCATGGTGTTGTCTAAATTAAAGGAAATTGCTGAAACTTATTTGGGTCATGATCATGAAGTGAACAAAGCTGTTATCACTGTTCCTGCTTACTTCAACAACTCACAGAGACAAGCTACAAAAGATGCAGGAAAAATTGCCGGTTTTAATGTGATGAGGATCATCAACGAGCCTACTGCAGCTGCTATTGCTTATGGTTTGGACAAGAAAATGTGGAGAGAAGGCGAGAAGAATGTTCTTGTCTTTGATCTTGGTGGTGGAACTTTTGATGTTTCCTTAGTGACTGTTGACGAAGGAATGTTCCAGGTTAAGGCCACTTTGGGAGATACTCATGTGGGTGGTGTTGATTTTGATAACAATTTGGTGAACCATCTTGTTGAATTATTTCATAGAAAGTACAAGAAGGATTTGAAGATCAGTGAGAATTCCAAAGCTTTGGGGAGGTTAAGATCAGCATGCGAGAAAGCAAAGAGGTTACTTTCTTCATCATCACAAACAGTTGTTGAGCTTGATTCACTATGTGAAGGGATTGATCTACATTTCACTGTTACTAGAGCTTTTTTTGAGGAAATAAACAAGGATTTGTTCAAAAAGTGCATGAAGACTGTGGAGAAGTGTTTGATTGAGGCAAAAATTAGTAAAAGAAAAGTTCATGAATTTGTTATTGTCGGAGGGTCTACTAGAATTCCGAAGATTCAACAACTACTGAAGGAAATGTTTAGTGTCAACGGCGAAGTCAAAGAGCCTTGCAAAAGCATTAATCCTGATGAAGCTGTGGCTTATGGCGCAGCAGTTCAAGCAGCAATATTGAATACTGAAGGAGACAAGAAGATTGAAGGCTTATTGCTGTTGGATGTTATGCCTTTTAGTCTTGGTGTTGAAACTAAAGGTGGTGTCATGTCTGTTTTGATTCCGAAGAATACTAGGATCCCTACAAAAAAGGAGAATGTTTTCTCCACAGCCCAAGGTAAAGTTTTGATCAAAGTCTATGAAGGAGAACATGCTAAGACTAAGGACAACTTTTTGCTTGGTAAATTTGAGCTCTCAGAATGCTCTTTAGTACCTAGAAAGGTTCCAAATATCAATATTTGTTTTGATGTTGATGTTAATGGAATTTTGGAGGTTACTGCTGAAGATAAGACAAAAGGGTTGAAACAGAAGATCATTATCACCAACAAGGAGGGAAGGTTGAGTCCTCAAGAGATGAGAAGAATGATGAGGGATGCCGAGCGGTATAAGGCAGAGGATGAGGAGGTTAGGAAGAAGGTGAAAGCAAAGAATTTGTTTGAGAATTATGTTTATGAAATGAGGGAGAGAGTGAGGAAGCTTGAAAAGGTAGTGGAGGAAAGTATAGAATGGCTTGACAGAAACCAATTGGCAGAAATTGATGAGTTTGAATTCAAGAAGCAGGAGTTGGAAAATAGTGTGAAGTTTCTTTAA
Protein sequence

>Msa0399210

MATRKSKAIGIDLGTSYSCIAVWRNNCVEIIPNDQGNRVTPSYVAFTDTERLIGDAAINQSAKNPHNTVFDAKRLIGHRFSDQSVQQDIRFWPFKVVPNHKDKPMIVVNYKGQEKHFSPQEISSMVLSKLKEIAETYLGHDHEVNKAVITVPAYFNNSQRQATKDAGKIAGFNVMRIINEPTAAAIAYGLDKKMWREGEKNVLVFDLGGGTFDVSLVTVDEGMFQVKATLGDTHVGGVDFDNNLVNHLVELFHRKYKKDLKISENSKALGRLRSACEKAKRLLSSSSQTVVELDSLCEGIDLHFTVTRAFFEEINKDLFKKCMKTVEKCLIEAKISKRKVHEFVIVGGSTRIPKIQQLLKEMFSVNGEVKEPCKSINPDEAVAYGAAVQAAILNTEGDKKIEGLLLLDVMPFSLGVETKGGVMSVLIPKNTRIPTKKENVFSTAQGKVLIKVYEGEHAKTKDNFLLGKFELSECSLVPRKVPNINICFDVDVNGILEVTAEDKTKGLKQKIIITNKEGRLSPQEMRRMMRDAERYKAEDEEVRKKVKAKNLFENYVYEMRERVRKLEKVVEESIEWLDRNQLAEIDEFEFKKQELENSVKFL*