AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1177060

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1177060 MtrunA17_Chr8g0347121 94.587 739 29 1 1 739 1 728 0.0 1414
Msa1177060 MtrunA17_Chr7g0275581 62.760 768 207 7 1 739 1 718 0.0 924
Msa1177060 MtrunA17_Chr3g0126541 56.975 767 248 11 1 739 1 713 0.0 838
Msa1177060 MtrunA17_Chr1g0158861 55.963 763 252 12 2 734 3 711 0.0 813
Msa1177060 MtrunA17_Chr4g0073941 48.902 683 295 8 55 734 94 725 0.0 657
Msa1177060 MtrunA17_Chr2g0326871 47.612 691 303 8 48 734 65 700 0.0 633
Msa1177060 MtrunA17_Chr2g0305771 47.025 706 295 13 57 739 40 689 0.0 625
Msa1177060 MtrunA17_Chr4g0001481 46.531 735 323 12 15 734 57 736 0.0 619
Msa1177060 MtrunA17_Chr4g0028861 47.181 674 292 11 1 666 66 683 0.0 615
Msa1177060 MtrunA17_Chr7g0228181 44.306 720 326 12 33 734 15 677 0.0 600
Msa1177060 MtrunA17_Chr3g0139071 44.193 706 304 9 60 734 73 719 0.0 597
Msa1177060 MtrunA17_Chr3g0125651 44.350 708 308 12 60 736 36 688 0.0 588
Msa1177060 MtrunA17_Chr1g0181121 43.950 719 334 13 34 734 11 678 0.0 587
Msa1177060 MtrunA17_Chr6g0481021 37.586 580 329 11 59 617 122 689 4.86e-116 365
Msa1177060 MtrunA17_Chr6g0480671 36.860 586 322 11 60 619 193 756 1.56e-109 350
Msa1177060 MtrunA17_Chr8g0360821 33.227 629 335 14 59 664 61 627 3.46e-107 341
Msa1177060 MtrunA17_Chr6g0480851 35.377 571 337 10 59 613 183 737 4.85e-106 341
Msa1177060 MtrunA17_Chr6g0480781 32.855 627 383 15 59 668 168 773 5.57e-99 322
Msa1177060 MtrunA17_Chr7g0218341 32.605 641 356 13 44 664 91 675 5.32e-93 305
Msa1177060 MtrunA17_Chr6g0480741 33.274 565 332 11 60 603 115 655 4.02e-88 291
Msa1177060 MtrunA17_Chr6g0480681 28.668 736 387 19 38 710 80 740 5.40e-78 265
Msa1177060 MtrunA17_Chr2g0318561 32.803 503 270 13 176 664 2 450 4.38e-76 254
Msa1177060 MtrunA17_Chr5g0395421 36.677 319 178 6 294 598 45 353 2.80e-50 180
Msa1177060 MtrunA17_Chr2g0326861 42.786 201 95 4 135 326 14 203 2.61e-41 150
Msa1177060 MtrunA17_Chr5g0395411 36.036 222 122 6 55 270 165 372 2.19e-38 147
Msa1177060 MtrunA17_Chr6g0480771 33.193 238 137 6 382 607 12 239 1.45e-28 116
Msa1177060 MtrunA17_Chr6g0480841 37.143 175 91 4 436 598 2 169 2.81e-25 104
Msa1177060 MtrunA17_Chr4g0063141 21.911 429 259 8 113 539 87 441 2.65e-22 102
Msa1177060 MtrunA17_Chr6g0480751 36.220 127 76 1 56 177 89 215 5.34e-22 95.5
Msa1177060 MtrunA17_Chr6g0480791 35.115 131 82 2 55 182 165 295 2.92e-21 95.1
Msa1177060 MtrunA17_Chr2g0277651 25.079 315 198 8 242 539 169 462 3.07e-17 86.3
Msa1177060 MtrunA17_Chr2g0318551 29.114 158 98 3 31 179 26 178 2.56e-15 75.9
Msa1177060 MtrunA17_Chr3g0138671 23.474 213 155 3 332 539 233 442 3.90e-14 76.3
Msa1177060 MtrunA17_Chr3g0138131 21.723 267 192 5 262 517 179 439 1.68e-11 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1177060 AT4G01010.1 57.965 747 229 10 9 734 9 691 0.0 842
Msa1177060 AT4G01010.2 57.965 747 229 10 9 734 9 691 0.0 842
Msa1177060 AT1G01340.2 58.592 710 239 7 32 734 42 703 0.0 827
Msa1177060 AT1G01340.1 58.592 710 239 7 32 734 37 698 0.0 827
Msa1177060 AT5G53130.1 56.955 762 250 11 1 734 1 712 0.0 824
Msa1177060 AT2G46430.1 52.497 741 275 11 23 734 9 701 0.0 743
Msa1177060 AT2G46430.2 52.497 741 275 11 23 734 9 701 0.0 743
Msa1177060 AT2G23980.6 49.017 712 300 10 41 739 59 720 0.0 688
Msa1177060 AT2G23980.9 49.017 712 300 10 41 739 86 747 0.0 687
Msa1177060 AT2G23980.10 49.017 712 300 10 41 739 86 747 0.0 687
Msa1177060 AT2G23980.1 49.017 712 300 10 41 739 86 747 0.0 687
Msa1177060 AT2G23980.4 49.017 712 300 10 41 739 86 747 0.0 687
Msa1177060 AT2G23980.5 49.017 712 300 10 41 739 86 747 0.0 687
Msa1177060 AT4G30560.1 50.366 683 273 10 60 734 103 727 0.0 682
Msa1177060 AT4G30560.4 50.366 683 273 10 60 734 103 727 0.0 682
Msa1177060 AT4G30560.5 50.366 683 273 10 60 734 77 701 0.0 682
Msa1177060 AT4G30560.2 50.366 683 273 10 60 734 77 701 0.0 682
Msa1177060 AT4G30560.3 50.366 683 273 10 60 734 77 701 0.0 682
Msa1177060 AT2G23980.3 47.619 735 299 11 41 739 86 770 0.0 675
Msa1177060 AT2G23980.2 47.619 735 299 11 41 739 86 770 0.0 675
Msa1177060 AT2G23980.8 47.619 735 299 11 41 739 86 770 0.0 675
Msa1177060 AT2G23980.7 47.619 735 299 11 41 739 86 770 0.0 675
Msa1177060 AT5G57940.1 47.066 767 320 12 1 734 1 714 0.0 669
Msa1177060 AT5G57940.2 47.066 767 320 12 1 734 1 714 0.0 669
Msa1177060 AT5G57940.3 50.000 680 282 8 60 734 81 707 0.0 667
Msa1177060 AT1G19780.1 48.857 700 281 9 60 734 97 744 0.0 658
Msa1177060 AT1G15990.1 45.860 785 318 15 1 734 7 735 0.0 657
Msa1177060 AT2G28260.1 47.989 696 281 12 48 737 58 678 0.0 618
Msa1177060 AT2G28260.2 47.989 696 281 12 48 737 18 638 0.0 618
Msa1177060 AT2G24610.1 44.892 744 303 13 39 734 38 722 0.0 617
Msa1177060 AT2G24610.2 44.892 744 303 13 39 734 53 737 0.0 615
Msa1177060 AT4G30360.1 45.106 705 308 13 59 739 70 719 0.0 589
Msa1177060 AT5G14870.1 42.997 714 328 10 57 738 37 703 0.0 585
Msa1177060 AT3G48010.1 42.657 715 322 13 57 734 41 704 0.0 576
Msa1177060 AT2G46440.1 47.634 634 278 9 68 697 37 620 0.0 552
Msa1177060 AT2G46450.4 43.452 672 311 10 68 734 37 644 0.0 536
Msa1177060 AT2G46450.1 43.452 672 311 10 68 734 37 644 0.0 536
Msa1177060 AT1G19780.2 47.945 584 227 9 176 734 2 533 8.14e-174 509
Msa1177060 AT2G46450.2 41.964 672 298 11 68 734 37 621 1.41e-170 504
Msa1177060 AT2G46450.3 41.964 672 298 11 68 734 24 608 1.97e-170 503
Msa1177060 AT2G46440.2 49.388 490 197 8 213 698 1 443 3.33e-144 430
Msa1177060 AT3G17690.1 33.174 627 346 12 57 669 153 720 1.27e-107 344
Msa1177060 AT3G17700.1 35.907 557 332 8 59 604 191 733 7.21e-105 338
Msa1177060 AT3G17690.2 35.685 496 297 8 57 546 153 632 1.55e-101 326
Msa1177060 AT3G17700.2 36.939 490 294 6 59 547 191 666 4.34e-99 321
Msa1177060 AT5G54250.2 31.994 622 354 14 60 664 79 648 6.95e-97 315
Msa1177060 AT5G54250.1 31.994 622 354 14 60 664 79 648 6.95e-97 315
Msa1177060 AT5G54250.4 31.994 622 354 14 60 664 79 648 6.95e-97 315
Msa1177060 AT5G15410.1 32.953 613 349 10 60 658 113 677 8.75e-95 310
Msa1177060 AT5G15410.2 32.953 613 349 10 60 658 112 676 1.09e-94 310
Msa1177060 AT5G54250.3 31.511 622 352 15 60 664 79 643 5.40e-92 302
Msa1177060 AT3G17690.3 32.708 373 236 6 57 423 153 516 1.21e-64 224
Msa1177060 AT2G26650.1 21.379 435 257 10 108 539 89 441 1.04e-16 85.1
Msa1177060 AT4G22200.2 24.402 209 155 2 331 539 258 463 3.44e-14 76.6
Msa1177060 AT2G26650.2 20.874 412 245 9 131 539 3 336 4.19e-14 76.6
Msa1177060 AT4G22200.1 24.402 209 155 2 331 539 258 463 9.09e-14 75.5

Find 138 sgRNAs with CRISPR-Local

Find 271 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTAATAAGACGTCGAAAT+TGG 0.155059 8_1:-9400855 None:intergenic
AACCAATCCTTTGTTATATA+TGG 0.163608 8_1:-9398773 None:intergenic
AAAGATCCTGATGATATTAT+TGG 0.173184 8_1:+9399213 Msa1177060:CDS
ATGTCTCTTCTAAGATCTTT+AGG 0.179678 8_1:-9400365 None:intergenic
GTTCTATTTGCATTACTTAT+AGG 0.188738 8_1:+9399782 Msa1177060:CDS
TTGTTAAGAAAGAGTGTTTA+TGG 0.195691 8_1:-9396665 None:intergenic
GCAATCTTCATTGCTGTCTT+TGG 0.196828 8_1:+9399755 Msa1177060:CDS
GGTGGGGTTTGCGCAATTTA+AGG 0.199611 8_1:+9399334 Msa1177060:CDS
TCAATGTTCCATTCAAATTA+AGG 0.206765 8_1:-9398169 None:intergenic
CAACATGGATAAACAGTTAT+TGG 0.209808 8_1:+9400519 Msa1177060:CDS
ATAAATTCTTCTACTGCTTT+TGG 0.244483 8_1:+9399313 Msa1177060:CDS
CATTTGTAGTCATGATTTAT+AGG 0.252028 8_1:-9397253 None:intergenic
AAGACCTCCAACACACTTTC+AGG 0.252666 8_1:+9400881 Msa1177060:CDS
ATTAGGTTGTTGGAGCATTT+TGG 0.256130 8_1:+9399046 Msa1177060:intron
CTGAAAACAAGCACTTATAT+TGG 0.281584 8_1:+9399719 Msa1177060:CDS
TGTCTCTTCTAAGATCTTTA+GGG 0.282154 8_1:-9400364 None:intergenic
CAATATGATTCTTCAATTGA+TGG 0.309646 8_1:+9397939 Msa1177060:CDS
TGAAAACAAGCACTTATATT+GGG 0.322349 8_1:+9399720 Msa1177060:CDS
ACGAAATGATGTATTATCTA+AGG 0.326371 8_1:-9400926 None:intergenic
AATTCTTCTTCATTCTGCTT+TGG 0.327794 8_1:+9396726 Msa1177060:five_prime_UTR
CGCGGTTCATACAAGTAATA+TGG 0.339598 8_1:+9401321 Msa1177060:CDS
TTCTGTTCAGATTTGAAGAT+TGG 0.339617 8_1:+9397886 Msa1177060:intron
GGAACTGAGCCTTGTGGAAT+TGG 0.348268 8_1:-9398752 None:intergenic
TCAACTGTACTAGTCGAAAT+AGG 0.351889 8_1:-9400772 None:intergenic
CTTCATTGCTGTCTTTGGAT+TGG 0.352622 8_1:+9399760 Msa1177060:CDS
TTTGCTGTTACCTTACCTAT+AGG 0.360777 8_1:-9396751 None:intergenic
TGCTTTACATTACTACTAGA+AGG 0.363681 8_1:+9401806 Msa1177060:three_prime_UTR
ACAGGGATGTAAAAGAATAA+TGG 0.368359 8_1:-9398125 None:intergenic
ATGTGTGATGGCAGTATCTA+TGG 0.374144 8_1:+9398100 Msa1177060:CDS
AAGTAATATGGCGTCGATAC+AGG 0.377837 8_1:+9401333 Msa1177060:CDS
CATTCTGCTTTGGTTCCTAT+AGG 0.377841 8_1:+9396736 Msa1177060:five_prime_UTR
ATCGTCTTTCAGTTTCGAAC+CGG 0.379722 8_1:+9398245 Msa1177060:CDS
GGAAAATGCCTTAATTTGAA+TGG 0.384868 8_1:+9398161 Msa1177060:CDS
GTGATGAAATCTGGCTCATC+TGG 0.387811 8_1:-9401554 None:intergenic
GGTAAGTTTGAAGAGGTTGT+AGG 0.390125 8_1:-9400751 None:intergenic
GTTAATAAGACGTCGAAATT+GGG 0.397762 8_1:-9400854 None:intergenic
ATCAATTCTTCCACTTCCTC+AGG 0.406342 8_1:+9398373 Msa1177060:CDS
CATCCCTGTGATTGATGACC+AGG 0.414426 8_1:+9398139 Msa1177060:CDS
TACTTGTGATATTAGGTTGT+TGG 0.414687 8_1:+9399036 Msa1177060:intron
CTCAAGACCGCCACAACAAA+TGG 0.419365 8_1:+9400646 Msa1177060:CDS
TCTCAAGTGCTTTAATGCAT+TGG 0.421438 8_1:-9401477 None:intergenic
AAGGTCCACTACTTCAGAAA+TGG 0.424933 8_1:+9398057 Msa1177060:CDS
ACAACAAATGGTGGAAGAAC+TGG 0.427072 8_1:+9400658 Msa1177060:CDS
AAATTTCACCTTTGGATTGG+AGG 0.428630 8_1:+9401005 Msa1177060:CDS
GGAGCTTCTAACATGGGCCT+TGG 0.434189 8_1:+9400726 Msa1177060:CDS
ATTCCAAAATTGAAAACTGT+TGG 0.436075 8_1:-9399237 None:intergenic
GAAGTGGAAGAATTGATAGA+AGG 0.438544 8_1:-9398367 None:intergenic
TTCCAAAATTGAAAACTGTT+GGG 0.439910 8_1:-9399236 None:intergenic
GTTGTTACTTGCAGTTCTGT+TGG 0.447707 8_1:+9399689 Msa1177060:intron
AATGAAGTAACAAGCACTTC+TGG 0.448752 8_1:+9398854 Msa1177060:CDS
TATCTCATTGCTATAGCAAA+AGG 0.451371 8_1:-9398320 None:intergenic
TGCTTTGGTTCCTATAGGTA+AGG 0.464653 8_1:+9396741 Msa1177060:five_prime_UTR
TCAGAAATTGTTACGTGAAA+CGG 0.470319 8_1:+9401367 Msa1177060:CDS
GACGGTATGAACAATACAAA+TGG 0.472315 8_1:+9400297 Msa1177060:CDS
TTGAAGAGATGAGAATCAAA+AGG 0.475729 8_1:+9400213 Msa1177060:CDS
TAGTACAGTTGAAACTATTA+CGG 0.479709 8_1:+9400783 Msa1177060:CDS
ATTCTTTGCTTCAAGTGATC+CGG 0.484052 8_1:-9400272 None:intergenic
TTGTTACTTGCAGTTCTGTT+GGG 0.485082 8_1:+9399690 Msa1177060:intron
ATTCCTTGCAATGGAGGACA+TGG 0.488633 8_1:+9401294 Msa1177060:CDS
ATGAAGAGCATTTCGTCCAC+TGG 0.491546 8_1:-9400610 None:intergenic
TTGTTCCATTTCTGAAGTAG+TGG 0.494112 8_1:-9398062 None:intergenic
GAATCGTCCGCAGCTCAGAT+AGG 0.505421 8_1:-9399142 None:intergenic
TTCCATATATAACAAAGGAT+TGG 0.506157 8_1:+9398771 Msa1177060:CDS
ATAGCAAAAGGATCGTCAAC+CGG 0.506487 8_1:-9398308 None:intergenic
TCGTCTTTCAGTTTCGAACC+GGG 0.507612 8_1:+9398246 Msa1177060:CDS
ACAAAATGCTTCTGAAAATG+AGG 0.510501 8_1:+9401400 Msa1177060:CDS
GTTGGGCCAATAATATCATC+AGG 0.511144 8_1:-9399219 None:intergenic
AACTGGTTTCTTTAACTCGA+CGG 0.515613 8_1:+9400675 Msa1177060:CDS
CGAAATAGGTAAGTTTGAAG+AGG 0.517146 8_1:-9400758 None:intergenic
TATTCTGATACCTGAGGAAG+TGG 0.517630 8_1:-9398383 None:intergenic
TTTCCCTGGTCATCAATCAC+AGG 0.520770 8_1:-9398143 None:intergenic
TAGAATCAGAGTTGCGGTGT+TGG 0.522839 8_1:+9399082 Msa1177060:CDS
TTAACTGAGACAGCATGGGC+TGG 0.523523 8_1:+9398881 Msa1177060:CDS
CTTGCCTGAAAGTGTGTTGG+AGG 0.524951 8_1:-9400885 None:intergenic
ATATCATCAGGATCTTTGTA+AGG 0.531313 8_1:-9399207 None:intergenic
AAAAGTTTCGTTGTTTGTGA+AGG 0.533061 8_1:+9400583 Msa1177060:CDS
AGTAATATGGCGTCGATACA+GGG 0.535014 8_1:+9401334 Msa1177060:CDS
TGCTCTAACGTCTCGTGTGG+TGG 0.535752 8_1:+9399269 Msa1177060:CDS
TTCTGAAGTAGTGGACCTTG+TGG 0.543372 8_1:-9398053 None:intergenic
CTCTCATGCATGATGACTTG+AGG 0.543393 8_1:+9400821 Msa1177060:CDS
GTAAGAATGTCTAGTGATTG+TGG 0.545657 8_1:+9401781 Msa1177060:three_prime_UTR
TCTGGTTTCTGAGGCAAAAG+TGG 0.546224 8_1:-9401536 None:intergenic
CTTTAACTCGACGGAACTCA+AGG 0.546626 8_1:+9400684 Msa1177060:CDS
AGATAGGAATCATCAAAGAA+TGG 0.547757 8_1:-9399126 None:intergenic
AAGAGATGAGAATCAAAAGG+AGG 0.548051 8_1:+9400216 Msa1177060:CDS
AGTTCTTCCACCATTTGTTG+TGG 0.548081 8_1:-9400656 None:intergenic
GATGTGCCACCGTATGCTAC+CGG 0.552256 8_1:+9400253 Msa1177060:CDS
GAGTCTTCTTCACCAAGCAT+TGG 0.553818 8_1:+9401425 Msa1177060:CDS
GCATTAAAGCACTTGAGAAG+TGG 0.554111 8_1:+9401482 Msa1177060:CDS
GTATAGATTCGCATAAGTCT+TGG 0.557040 8_1:-9398824 None:intergenic
CTCCAACACACTTTCAGGCA+AGG 0.557764 8_1:+9400886 Msa1177060:CDS
TCCTTGCAATGGAGGACATG+GGG 0.558163 8_1:+9401296 Msa1177060:CDS
AGAACACTGCACCTAGAAGC+TGG 0.559017 8_1:-9398026 None:intergenic
TTTAACTATAGTAGTTGTGT+AGG 0.560518 8_1:+9401678 Msa1177060:three_prime_UTR
AGAGATGAGAATCAAAAGGA+GGG 0.561003 8_1:+9400217 Msa1177060:CDS
TGAAGAGGTTGTAGGATCCA+AGG 0.563224 8_1:-9400743 None:intergenic
TCAAGTGATCCGGTAGCATA+CGG 0.569335 8_1:-9400262 None:intergenic
AACTCGACGGAACTCAAGGC+TGG 0.573776 8_1:+9400688 Msa1177060:CDS
TTCCTTGCAATGGAGGACAT+GGG 0.577231 8_1:+9401295 Msa1177060:CDS
TCCCCATGTCCTCCATTGCA+AGG 0.577850 8_1:-9401297 None:intergenic
TATACAGTGGAACCAATGCT+TGG 0.578069 8_1:-9401437 None:intergenic
GTGGAGAGGAGCTTCTAACA+TGG 0.578654 8_1:+9400719 Msa1177060:CDS
TCTGGCTCATCTGGTTTCTG+AGG 0.580159 8_1:-9401545 None:intergenic
GTTCCACTGTATATGTACAA+AGG 0.580611 8_1:+9401447 Msa1177060:CDS
ATTACTTATAGGAAACATGC+AGG 0.580930 8_1:+9399793 Msa1177060:CDS
CTCTTCTAAGATCTTTAGGG+AGG 0.583580 8_1:-9400361 None:intergenic
ATAAACACTCTTTCTTAACA+AGG 0.586255 8_1:+9396667 Msa1177060:five_prime_UTR
TCTTCCACCATTTGTTGTGG+CGG 0.589608 8_1:-9400653 None:intergenic
CTCAGTTCCATATATAACAA+AGG 0.591567 8_1:+9398766 Msa1177060:CDS
AGCCTTGCCTGAAAGTGTGT+TGG 0.594824 8_1:-9400888 None:intergenic
AAAGGAGGGATGCAGAACAA+TGG 0.601963 8_1:+9400231 Msa1177060:CDS
GTATATTAACTGAGACAGCA+TGG 0.602058 8_1:+9398876 Msa1177060:CDS
CGAAGAAGGTGCAATAAACC+CGG 0.603746 8_1:-9398264 None:intergenic
TGGAGAGGAGCTTCTAACAT+GGG 0.608318 8_1:+9400720 Msa1177060:CDS
GAAAACAAGCACTTATATTG+GGG 0.608622 8_1:+9399721 Msa1177060:CDS
TTCCCTGGTCATCAATCACA+GGG 0.611426 8_1:-9398142 None:intergenic
GATGATTCCTATCTGAGCTG+CGG 0.614958 8_1:+9399135 Msa1177060:CDS
TATATTAACTGAGACAGCAT+GGG 0.622764 8_1:+9398877 Msa1177060:CDS
AATGGAGGACATGGGGAGCG+CGG 0.630933 8_1:+9401303 Msa1177060:CDS
CAAATGAAAAGAAATTTGTG+AGG 0.631583 8_1:+9397270 Msa1177060:CDS
AAGACCGCCACAACAAATGG+TGG 0.633558 8_1:+9400649 Msa1177060:CDS
CTTTGTTATAACATGTGTGA+TGG 0.634441 8_1:+9398088 Msa1177060:CDS
CAGTTACGCCTCCAATCCAA+AGG 0.644171 8_1:-9401013 None:intergenic
TTTCGATAGAATCAGAGTTG+CGG 0.645163 8_1:+9399076 Msa1177060:CDS
AATGTGTGATAAACTGAAGC+CGG 0.650306 8_1:+9400546 Msa1177060:CDS
ACTTGAAGCAAAGAATTCGA+CGG 0.656032 8_1:+9400279 Msa1177060:CDS
AATTTACATTATAAGAAACA+AGG 0.663822 8_1:+9400961 Msa1177060:CDS
TGCAGTGTTCTTGATCCACA+AGG 0.664587 8_1:+9398038 Msa1177060:CDS
GCAATGATTCCAATTCCACA+AGG 0.673079 8_1:+9398743 Msa1177060:CDS
TGATGCTCTAACGTCTCGTG+TGG 0.679720 8_1:+9399266 Msa1177060:CDS
TGTTTGTGAAGGTGATCCAG+TGG 0.682601 8_1:+9400594 Msa1177060:CDS
ATCCCTGTGATTGATGACCA+GGG 0.686356 8_1:+9398140 Msa1177060:CDS
AGGCACAAATAACTTGCTAG+TGG 0.686994 8_1:+9401698 Msa1177060:three_prime_UTR
TACAAATGGCAAGAAAATCG+AGG 0.692084 8_1:+9400311 Msa1177060:CDS
GAAATGCTCTTCATCATGCG+CGG 0.701329 8_1:+9400619 Msa1177060:CDS
AAACCTTTGTACATATACAG+TGG 0.715371 8_1:-9401450 None:intergenic
ATATATGGAACTGAGCCTTG+TGG 0.739194 8_1:-9398758 None:intergenic
AGTGATCCGGTAGCATACGG+TGG 0.739209 8_1:-9400259 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATTAGTTAAAAATATCT+TGG - chr8_1:9396884-9396903 None:intergenic 10.0%
!! TACATAATAAATAATGAAAT+TGG + chr8_1:9397070-9397089 Msa1177060:intron 10.0%
!! AAAATATATGATATATCATA+TGG + chr8_1:9397743-9397762 Msa1177060:intron 10.0%
!! CTAATTAATATTTCTTCTTA+TGG + chr8_1:9396896-9396915 Msa1177060:intron 15.0%
!!! AAAATTCTAATTTTTGAAGA+TGG + chr8_1:9397173-9397192 Msa1177060:intron 15.0%
!! TAAGTTACCAATATATTAAA+AGG + chr8_1:9398500-9398519 Msa1177060:intron 15.0%
!! AATTTACATTATAAGAAACA+AGG + chr8_1:9400961-9400980 Msa1177060:CDS 15.0%
!!! AGTATTTTTATTACTATATG+AGG + chr8_1:9401178-9401197 Msa1177060:intron 15.0%
!! CTGATTAAAAAGAATATGTA+TGG - chr8_1:9397363-9397382 None:intergenic 20.0%
!! ATAAACTAAGCATAAATCTA+TGG + chr8_1:9397770-9397789 Msa1177060:intron 20.0%
!! AAATTGAAAAATAACCAACT+AGG - chr8_1:9397800-9397819 None:intergenic 20.0%
!!! AATGATTCATTATTTTTTGC+TGG - chr8_1:9397999-9398018 None:intergenic 20.0%
!! AGCAAAAAATAATGAATCAT+TGG + chr8_1:9397998-9398017 Msa1177060:CDS 20.0%
!! AGAATATGCTTGAATAAAAA+AGG - chr8_1:9398414-9398433 None:intergenic 20.0%
!!! TTTTATTCAAGCATATTCTA+AGG + chr8_1:9398415-9398434 Msa1177060:intron 20.0%
!!! AAGTTCACCTTTTAATATAT+TGG - chr8_1:9398510-9398529 None:intergenic 20.0%
!! TAAGAGCTTATATAAATGTT+TGG - chr8_1:9399542-9399561 None:intergenic 20.0%
!! TGTCACAAAATATTAAATAG+TGG - chr8_1:9399623-9399642 None:intergenic 20.0%
!! AACTAAACAGGTTAAATTAT+AGG + chr8_1:9399964-9399983 Msa1177060:intron 20.0%
!!! ATATCTTGTTTACTTAAGTA+AGG - chr8_1:9400022-9400041 None:intergenic 20.0%
!!! TTCATACTTTTCTATGTTAT+CGG - chr8_1:9401078-9401097 None:intergenic 20.0%
!! AAAATAGAAGAAAGACAATA+TGG + chr8_1:9401233-9401252 Msa1177060:intron 20.0%
!!! TTGTTAAGAAAGAGTGTTTA+TGG - chr8_1:9396668-9396687 None:intergenic 25.0%
! ATAAACACTCTTTCTTAACA+AGG + chr8_1:9396667-9396686 Msa1177060:five_prime_UTR 25.0%
! CACACATAACTAGAAAAAAA+AGG - chr8_1:9397147-9397166 None:intergenic 25.0%
! CATTTGTAGTCATGATTTAT+AGG - chr8_1:9397256-9397275 None:intergenic 25.0%
! CAAATGAAAAGAAATTTGTG+AGG + chr8_1:9397270-9397289 Msa1177060:CDS 25.0%
!!! TTTGTTTTGTTCAGGAAAAA+AGG + chr8_1:9397397-9397416 Msa1177060:intron 25.0%
! TAAAATTGCCTATACGAATT+CGG + chr8_1:9397459-9397478 Msa1177060:intron 25.0%
! TCAAAATCTGCACTAAAATA+TGG - chr8_1:9397559-9397578 None:intergenic 25.0%
! ACTACGTAAATTCTTACAAT+CGG + chr8_1:9397654-9397673 Msa1177060:intron 25.0%
! TTTAGTCACTCATAGTAATA+TGG - chr8_1:9397726-9397745 None:intergenic 25.0%
! CAGAAATATAATCTCAGTTA+AGG - chr8_1:9397871-9397890 None:intergenic 25.0%
! CAATATGATTCTTCAATTGA+TGG + chr8_1:9397939-9397958 Msa1177060:CDS 25.0%
! TCAATGTTCCATTCAAATTA+AGG - chr8_1:9398172-9398191 None:intergenic 25.0%
! TTCCATATATAACAAAGGAT+TGG + chr8_1:9398771-9398790 Msa1177060:CDS 25.0%
!! AACCAATCCTTTGTTATATA+TGG - chr8_1:9398776-9398795 None:intergenic 25.0%
! AAAACTTAGCTTAGTAAACA+AGG - chr8_1:9398998-9399017 None:intergenic 25.0%
! TCCAAATTACTTGTGATATT+AGG + chr8_1:9399029-9399048 Msa1177060:intron 25.0%
! ACCTAATATCACAAGTAATT+TGG - chr8_1:9399033-9399052 None:intergenic 25.0%
! AAAGATCCTGATGATATTAT+TGG + chr8_1:9399213-9399232 Msa1177060:CDS 25.0%
! TTCCAAAATTGAAAACTGTT+GGG - chr8_1:9399239-9399258 None:intergenic 25.0%
! ATTCCAAAATTGAAAACTGT+TGG - chr8_1:9399240-9399259 None:intergenic 25.0%
!! ATAAATTCTTCTACTGCTTT+TGG + chr8_1:9399313-9399332 Msa1177060:CDS 25.0%
!! TATTTTGTGACATCTTATCA+AGG + chr8_1:9399631-9399650 Msa1177060:intron 25.0%
! TGAAAACAAGCACTTATATT+GGG + chr8_1:9399720-9399739 Msa1177060:CDS 25.0%
! GTTCTATTTGCATTACTTAT+AGG + chr8_1:9399782-9399801 Msa1177060:CDS 25.0%
! AGATAATCGTAGAAATAAGT+CGG + chr8_1:9399841-9399860 Msa1177060:intron 25.0%
!!! ATAAGCTTTGAAAAAAGTCT+TGG - chr8_1:9399942-9399961 None:intergenic 25.0%
! CAAAGCTTATTTAACTAAAC+AGG + chr8_1:9399952-9399971 Msa1177060:intron 25.0%
!! AGGCTATCAAAAAACATTTT+AGG + chr8_1:9399984-9400003 Msa1177060:intron 25.0%
! ATCATGAATCATTTATCTGT+TGG + chr8_1:9400102-9400121 Msa1177060:intron 25.0%
! CATAAACAAGTATAGTTAAG+TGG - chr8_1:9400474-9400493 None:intergenic 25.0%
! TAGTACAGTTGAAACTATTA+CGG + chr8_1:9400783-9400802 Msa1177060:CDS 25.0%
! ACGAAATGATGTATTATCTA+AGG - chr8_1:9400929-9400948 None:intergenic 25.0%
! TCATCTAAAAATTTCACCTT+TGG + chr8_1:9400997-9401016 Msa1177060:CDS 25.0%
! TAAAAATTTCACCTTTGGAT+TGG + chr8_1:9401002-9401021 Msa1177060:CDS 25.0%
!! ACAATAGTAATTTTCGAAAC+TGG - chr8_1:9401146-9401165 None:intergenic 25.0%
! CGAAAATTACTATTGTATTG+TGG + chr8_1:9401150-9401169 Msa1177060:intron 25.0%
!!! TTTTTTTGAGTGATGAAATC+TGG - chr8_1:9401566-9401585 None:intergenic 25.0%
! TTTAACTATAGTAGTTGTGT+AGG + chr8_1:9401678-9401697 Msa1177060:three_prime_UTR 25.0%
AATTCTTCTTCATTCTGCTT+TGG + chr8_1:9396726-9396745 Msa1177060:five_prime_UTR 30.0%
ACATTTATCAAAACCAACCT+TGG + chr8_1:9396787-9396806 Msa1177060:intron 30.0%
GAAATAGTAGAAGGAAAAGA+AGG - chr8_1:9396835-9396854 None:intergenic 30.0%
GATAAGTGTGAAATAGTAGA+AGG - chr8_1:9396844-9396863 None:intergenic 30.0%
!! TTTTTGAAGATGGATTTTGC+AGG + chr8_1:9397183-9397202 Msa1177060:intron 30.0%
!!! GTGATTCATTTGTTTTGTTC+AGG + chr8_1:9397389-9397408 Msa1177060:intron 30.0%
AACATCTGATCTCTATTCAA+AGG + chr8_1:9397617-9397636 Msa1177060:intron 30.0%
! GAACTACTAACTTGTGAATT+CGG - chr8_1:9397694-9397713 None:intergenic 30.0%
! AAATCTATGGTTTTCCTAGT+TGG + chr8_1:9397783-9397802 Msa1177060:intron 30.0%
TTCTGTTCAGATTTGAAGAT+TGG + chr8_1:9397886-9397905 Msa1177060:intron 30.0%
! CTTTGTTATAACATGTGTGA+TGG + chr8_1:9398088-9398107 Msa1177060:CDS 30.0%
ACAGGGATGTAAAAGAATAA+TGG - chr8_1:9398128-9398147 None:intergenic 30.0%
! GGAAAATGCCTTAATTTGAA+TGG + chr8_1:9398161-9398180 Msa1177060:CDS 30.0%
TATCTCATTGCTATAGCAAA+AGG - chr8_1:9398323-9398342 None:intergenic 30.0%
! ATATTCTAAGGAGTTCACTA+AGG + chr8_1:9398427-9398446 Msa1177060:intron 30.0%
TTAACTAATGCTCTTAAGCA+TGG + chr8_1:9398589-9398608 Msa1177060:intron 30.0%
CTCAGTTCCATATATAACAA+AGG + chr8_1:9398766-9398785 Msa1177060:CDS 30.0%
TATATTAACTGAGACAGCAT+GGG + chr8_1:9398877-9398896 Msa1177060:CDS 30.0%
! TACTTGTGATATTAGGTTGT+TGG + chr8_1:9399036-9399055 Msa1177060:intron 30.0%
AGATAGGAATCATCAAAGAA+TGG - chr8_1:9399129-9399148 None:intergenic 30.0%
ATATCATCAGGATCTTTGTA+AGG - chr8_1:9399210-9399229 None:intergenic 30.0%
GTTTACTCTATAAGAACACA+TGG - chr8_1:9399397-9399416 None:intergenic 30.0%
GAGCTTATATAAATGTTTGG+AGG - chr8_1:9399539-9399558 None:intergenic 30.0%
AAACACTGCGAATAAAGTTA+AGG - chr8_1:9399589-9399608 None:intergenic 30.0%
!! CAATATAAGTGCTTGTTTTC+AGG - chr8_1:9399721-9399740 None:intergenic 30.0%
CTGAAAACAAGCACTTATAT+TGG + chr8_1:9399719-9399738 Msa1177060:CDS 30.0%
GAAAACAAGCACTTATATTG+GGG + chr8_1:9399721-9399740 Msa1177060:CDS 30.0%
ATTACTTATAGGAAACATGC+AGG + chr8_1:9399793-9399812 Msa1177060:CDS 30.0%
AGTACACTATATATGTGACT+AGG - chr8_1:9400080-9400099 None:intergenic 30.0%
TTGAAGAGATGAGAATCAAA+AGG + chr8_1:9400213-9400232 Msa1177060:CDS 30.0%
!! TGTCTCTTCTAAGATCTTTA+GGG - chr8_1:9400367-9400386 None:intergenic 30.0%
!! ATGTCTCTTCTAAGATCTTT+AGG - chr8_1:9400368-9400387 None:intergenic 30.0%
TAGTACTTTCATCTTTCACT+TGG + chr8_1:9400436-9400455 Msa1177060:intron 30.0%
TTATCCATGTTGCCAAAAAT+TGG - chr8_1:9400511-9400530 None:intergenic 30.0%
CAACATGGATAAACAGTTAT+TGG + chr8_1:9400519-9400538 Msa1177060:CDS 30.0%
! ACAAACAACGAAACTTTTCT+CGG - chr8_1:9400581-9400600 None:intergenic 30.0%
AAAAGTTTCGTTGTTTGTGA+AGG + chr8_1:9400583-9400602 Msa1177060:CDS 30.0%
GTTAATAAGACGTCGAAATT+GGG - chr8_1:9400857-9400876 None:intergenic 30.0%
TGTTAATAAGACGTCGAAAT+TGG - chr8_1:9400858-9400877 None:intergenic 30.0%
! TTCGAAACTGGTTTATATTG+CGG - chr8_1:9401134-9401153 None:intergenic 30.0%
TCATCCTAATATCTTTACCA+TGG - chr8_1:9401214-9401233 None:intergenic 30.0%
TCAGAAATTGTTACGTGAAA+CGG + chr8_1:9401367-9401386 Msa1177060:CDS 30.0%
! ACAAAATGCTTCTGAAAATG+AGG + chr8_1:9401400-9401419 Msa1177060:CDS 30.0%
AAACCTTTGTACATATACAG+TGG - chr8_1:9401453-9401472 None:intergenic 30.0%
TGCTTTACATTACTACTAGA+AGG + chr8_1:9401806-9401825 Msa1177060:three_prime_UTR 30.0%
TCAATGAGTGCACAAAAAAT+TGG - chr8_1:9401832-9401851 None:intergenic 30.0%
!!! ATTTTTTGTGCACTCATTGA+TGG + chr8_1:9401832-9401851 Msa1177060:three_prime_UTR 30.0%
!! TTTGCTGTTACCTTACCTAT+AGG - chr8_1:9396754-9396773 None:intergenic 35.0%
TTTATCAAAACCAACCTTGG+TGG + chr8_1:9396790-9396809 Msa1177060:intron 35.0%
GTAGAAGGAAAAGAAGGAAA+AGG - chr8_1:9396829-9396848 None:intergenic 35.0%
! GTATAACTATAAGTGCTGCA+TGG - chr8_1:9397111-9397130 None:intergenic 35.0%
TGTATGGCAACTGAAACAAT+AGG - chr8_1:9397347-9397366 None:intergenic 35.0%
GAATAGAGATCAGATGTTTC+TGG - chr8_1:9397615-9397634 None:intergenic 35.0%
AATTTACGTAGTCAGCACAT+CGG - chr8_1:9397646-9397665 None:intergenic 35.0%
TTGTGAATTCGGATTCTTTG+AGG - chr8_1:9397683-9397702 None:intergenic 35.0%
!!! TTTTTTGCTGGAGTAACACT+TGG - chr8_1:9397987-9398006 None:intergenic 35.0%
!! TTGTTCCATTTCTGAAGTAG+TGG - chr8_1:9398065-9398084 None:intergenic 35.0%
!! TCAAATTAAGGCATTTTCCC+TGG - chr8_1:9398160-9398179 None:intergenic 35.0%
GAAGTGGAAGAATTGATAGA+AGG - chr8_1:9398370-9398389 None:intergenic 35.0%
GTACTATATTCTGATACCTG+AGG - chr8_1:9398392-9398411 None:intergenic 35.0%
! CGTAATGATGTGTTGATAGA+TGG + chr8_1:9398712-9398731 Msa1177060:intron 35.0%
GTATAGATTCGCATAAGTCT+TGG - chr8_1:9398827-9398846 None:intergenic 35.0%
AATGAAGTAACAAGCACTTC+TGG + chr8_1:9398854-9398873 Msa1177060:CDS 35.0%
GTATATTAACTGAGACAGCA+TGG + chr8_1:9398876-9398895 Msa1177060:CDS 35.0%
!! ATTAGGTTGTTGGAGCATTT+TGG + chr8_1:9399046-9399065 Msa1177060:intron 35.0%
TTTCGATAGAATCAGAGTTG+CGG + chr8_1:9399076-9399095 Msa1177060:CDS 35.0%
!! AATTCTTCTACTGCTTTTGG+TGG + chr8_1:9399316-9399335 Msa1177060:CDS 35.0%
!! ATTCTTCTACTGCTTTTGGT+GGG + chr8_1:9399317-9399336 Msa1177060:CDS 35.0%
! TTGTTACTTGCAGTTCTGTT+GGG + chr8_1:9399690-9399709 Msa1177060:intron 35.0%
AGGTAATCCTTAGTAAGGAA+GGG + chr8_1:9399813-9399832 Msa1177060:intron 35.0%
! CTTTGAAAAAAGTCTTGGCA+TGG - chr8_1:9399937-9399956 None:intergenic 35.0%
CTTAAGTAAGGTGACTTGAT+AGG - chr8_1:9400010-9400029 None:intergenic 35.0%
AAGAGATGAGAATCAAAAGG+AGG + chr8_1:9400216-9400235 Msa1177060:CDS 35.0%
AGAGATGAGAATCAAAAGGA+GGG + chr8_1:9400217-9400236 Msa1177060:CDS 35.0%
ATTCTTTGCTTCAAGTGATC+CGG - chr8_1:9400275-9400294 None:intergenic 35.0%
ACTTGAAGCAAAGAATTCGA+CGG + chr8_1:9400279-9400298 Msa1177060:CDS 35.0%
GACGGTATGAACAATACAAA+TGG + chr8_1:9400297-9400316 Msa1177060:CDS 35.0%
TACAAATGGCAAGAAAATCG+AGG + chr8_1:9400311-9400330 Msa1177060:CDS 35.0%
ACTTGTTTATGTGTGATGAC+AGG + chr8_1:9400483-9400502 Msa1177060:intron 35.0%
AATGTGTGATAAACTGAAGC+CGG + chr8_1:9400546-9400565 Msa1177060:CDS 35.0%
! CGAAACTTTTCTCGGTATAA+AGG - chr8_1:9400573-9400592 None:intergenic 35.0%
! AACTGGTTTCTTTAACTCGA+CGG + chr8_1:9400675-9400694 Msa1177060:CDS 35.0%
CGAAATAGGTAAGTTTGAAG+AGG - chr8_1:9400761-9400780 None:intergenic 35.0%
TCAACTGTACTAGTCGAAAT+AGG - chr8_1:9400775-9400794 None:intergenic 35.0%
! AAATTTCACCTTTGGATTGG+AGG + chr8_1:9401005-9401024 Msa1177060:CDS 35.0%
GAGACCATGGTAAAGATATT+AGG + chr8_1:9401207-9401226 Msa1177060:intron 35.0%
CTCAAACATTGTCATCAATG+AGG - chr8_1:9401266-9401285 None:intergenic 35.0%
! ATTGATGACAATGTTTGAGC+AGG + chr8_1:9401267-9401286 Msa1177060:intron 35.0%
TGTTACGTGAAACGGAAAAA+AGG + chr8_1:9401375-9401394 Msa1177060:CDS 35.0%
GTTCCACTGTATATGTACAA+AGG + chr8_1:9401447-9401466 Msa1177060:CDS 35.0%
TCTCAAGTGCTTTAATGCAT+TGG - chr8_1:9401480-9401499 None:intergenic 35.0%
GTAAGAATGTCTAGTGATTG+TGG + chr8_1:9401781-9401800 Msa1177060:three_prime_UTR 35.0%
! CATTCTGCTTTGGTTCCTAT+AGG + chr8_1:9396736-9396755 Msa1177060:five_prime_UTR 40.0%
! TGCTTTGGTTCCTATAGGTA+AGG + chr8_1:9396741-9396760 Msa1177060:five_prime_UTR 40.0%
GAAAAACAGTAACACCACCA+AGG - chr8_1:9396807-9396826 None:intergenic 40.0%
TCCATATCAACCTGAGAGAA+GGG - chr8_1:9396937-9396956 None:intergenic 40.0%
CAGGAAATCCGAATTCGTAT+AGG - chr8_1:9397470-9397489 None:intergenic 40.0%
ACTACGTGAATGAGTGCAAT+CGG - chr8_1:9397499-9397518 None:intergenic 40.0%
AAATATGGACCGTTCGATGT+CGG - chr8_1:9397544-9397563 None:intergenic 40.0%
CATTGGAAAAACCAGCTTCT+AGG + chr8_1:9398015-9398034 Msa1177060:CDS 40.0%
AAGGTCCACTACTTCAGAAA+TGG + chr8_1:9398057-9398076 Msa1177060:CDS 40.0%
ATGTGTGATGGCAGTATCTA+TGG + chr8_1:9398100-9398119 Msa1177060:CDS 40.0%
ATCGTCTTTCAGTTTCGAAC+CGG + chr8_1:9398245-9398264 Msa1177060:CDS 40.0%
!! TTCTTCGCGCATTTTTGGAA+GGG + chr8_1:9398280-9398299 Msa1177060:CDS 40.0%
ATAGCAAAAGGATCGTCAAC+CGG - chr8_1:9398311-9398330 None:intergenic 40.0%
ATCAATTCTTCCACTTCCTC+AGG + chr8_1:9398373-9398392 Msa1177060:CDS 40.0%
TATTCTGATACCTGAGGAAG+TGG - chr8_1:9398386-9398405 None:intergenic 40.0%
AGCATGGCTAACAGTATTGT+AGG + chr8_1:9398605-9398624 Msa1177060:intron 40.0%
GCAATGATTCCAATTCCACA+AGG + chr8_1:9398743-9398762 Msa1177060:CDS 40.0%
ATATATGGAACTGAGCCTTG+TGG - chr8_1:9398761-9398780 None:intergenic 40.0%
GTTGGGCCAATAATATCATC+AGG - chr8_1:9399222-9399241 None:intergenic 40.0%
! GGCCCAACAGTTTTCAATTT+TGG + chr8_1:9399234-9399253 Msa1177060:CDS 40.0%
!! TTCTTCTACTGCTTTTGGTG+GGG + chr8_1:9399318-9399337 Msa1177060:CDS 40.0%
GTTGTTGATCTTCTCTGCTT+AGG + chr8_1:9399360-9399379 Msa1177060:intron 40.0%
CTTTATTCGCAGTGTTTCGA+TGG + chr8_1:9399592-9399611 Msa1177060:intron 40.0%
! GTTGTTACTTGCAGTTCTGT+TGG + chr8_1:9399689-9399708 Msa1177060:intron 40.0%
GCAATCTTCATTGCTGTCTT+TGG + chr8_1:9399755-9399774 Msa1177060:CDS 40.0%
CTTCATTGCTGTCTTTGGAT+TGG + chr8_1:9399760-9399779 Msa1177060:CDS 40.0%
CATGCAGGTAATCCTTAGTA+AGG + chr8_1:9399808-9399827 Msa1177060:intron 40.0%
CAGGTAATCCTTAGTAAGGA+AGG + chr8_1:9399812-9399831 Msa1177060:intron 40.0%
! ATAAGTCGGACTGACTTACA+AGG + chr8_1:9399855-9399874 Msa1177060:intron 40.0%
! TAAGTCGGACTGACTTACAA+GGG + chr8_1:9399856-9399875 Msa1177060:intron 40.0%
!! CTCTTCTAAGATCTTTAGGG+AGG - chr8_1:9400364-9400383 None:intergenic 40.0%
! TGATGACAGGTGCCAATTTT+TGG + chr8_1:9400496-9400515 Msa1177060:intron 40.0%
! CTTTTCTCGGTATAAAGGAC+CGG - chr8_1:9400568-9400587 None:intergenic 40.0%
! AGTTCTTCCACCATTTGTTG+TGG - chr8_1:9400659-9400678 None:intergenic 40.0%
! ACAACAAATGGTGGAAGAAC+TGG + chr8_1:9400658-9400677 Msa1177060:CDS 40.0%
GGTAAGTTTGAAGAGGTTGT+AGG - chr8_1:9400754-9400773 None:intergenic 40.0%
TATGAGGAAATGTGAGACCA+TGG + chr8_1:9401194-9401213 Msa1177060:intron 40.0%
!!! GCAGGTTTTATTCCTTGCAA+TGG + chr8_1:9401285-9401304 Msa1177060:intron 40.0%
!!! GGTTTTATTCCTTGCAATGG+AGG + chr8_1:9401288-9401307 Msa1177060:intron 40.0%
CGCGGTTCATACAAGTAATA+TGG + chr8_1:9401321-9401340 Msa1177060:CDS 40.0%
!! AAGTAATATGGCGTCGATAC+AGG + chr8_1:9401333-9401352 Msa1177060:CDS 40.0%
!! AGTAATATGGCGTCGATACA+GGG + chr8_1:9401334-9401353 Msa1177060:CDS 40.0%
TATACAGTGGAACCAATGCT+TGG - chr8_1:9401440-9401459 None:intergenic 40.0%
! GCATTAAAGCACTTGAGAAG+TGG + chr8_1:9401482-9401501 Msa1177060:CDS 40.0%
!! AGGCACAAATAACTTGCTAG+TGG + chr8_1:9401698-9401717 Msa1177060:three_prime_UTR 40.0%
AACAGTAACACCACCAAGGT+TGG - chr8_1:9396803-9396822 None:intergenic 45.0%
TATCAACCTGAGAGAAGGGT+TGG - chr8_1:9396933-9396952 None:intergenic 45.0%
!! ACCCTTCTCTCAGGTTGATA+TGG + chr8_1:9396933-9396952 Msa1177060:intron 45.0%
CTCCATATCAACCTGAGAGA+AGG - chr8_1:9396938-9396957 None:intergenic 45.0%
CATTCACGTAGTCAGCACAT+CGG + chr8_1:9397507-9397526 Msa1177060:intron 45.0%
TGCAGTGTTCTTGATCCACA+AGG + chr8_1:9398038-9398057 Msa1177060:CDS 45.0%
!! TTCTGAAGTAGTGGACCTTG+TGG - chr8_1:9398056-9398075 None:intergenic 45.0%
! ATCCCTGTGATTGATGACCA+GGG + chr8_1:9398140-9398159 Msa1177060:CDS 45.0%
TTCCCTGGTCATCAATCACA+GGG - chr8_1:9398145-9398164 None:intergenic 45.0%
TTTCCCTGGTCATCAATCAC+AGG - chr8_1:9398146-9398165 None:intergenic 45.0%
TCGTCTTTCAGTTTCGAACC+GGG + chr8_1:9398246-9398265 Msa1177060:CDS 45.0%
! CGAAGAAGGTGCAATAAACC+CGG - chr8_1:9398267-9398286 None:intergenic 45.0%
CTTCCAAAAATGCGCGAAGA+AGG - chr8_1:9398281-9398300 None:intergenic 45.0%
!! CTTCTTCGCGCATTTTTGGA+AGG + chr8_1:9398279-9398298 Msa1177060:CDS 45.0%
!! TCTTCGCGCATTTTTGGAAG+GGG + chr8_1:9398281-9398300 Msa1177060:CDS 45.0%
TAGAATCAGAGTTGCGGTGT+TGG + chr8_1:9399082-9399101 Msa1177060:CDS 45.0%
GATGATTCCTATCTGAGCTG+CGG + chr8_1:9399135-9399154 Msa1177060:CDS 45.0%
! GGGTTTGCGCAATTTAAGGT+TGG + chr8_1:9399338-9399357 Msa1177060:intron 45.0%
TCTGATACAGACTAGGTCGT+AGG - chr8_1:9399882-9399901 None:intergenic 45.0%
ACACGTGTCTGATACAGACT+AGG - chr8_1:9399889-9399908 None:intergenic 45.0%
AAAGGAGGGATGCAGAACAA+TGG + chr8_1:9400231-9400250 Msa1177060:CDS 45.0%
TCAAGTGATCCGGTAGCATA+CGG - chr8_1:9400265-9400284 None:intergenic 45.0%
!!! GGTGCCAATTTTTGGCAACA+TGG + chr8_1:9400504-9400523 Msa1177060:intron 45.0%
TGTTTGTGAAGGTGATCCAG+TGG + chr8_1:9400594-9400613 Msa1177060:CDS 45.0%
!! ATGAAGAGCATTTCGTCCAC+TGG - chr8_1:9400613-9400632 None:intergenic 45.0%
GAAATGCTCTTCATCATGCG+CGG + chr8_1:9400619-9400638 Msa1177060:CDS 45.0%
!! TCTTCCACCATTTGTTGTGG+CGG - chr8_1:9400656-9400675 None:intergenic 45.0%
CTTTAACTCGACGGAACTCA+AGG + chr8_1:9400684-9400703 Msa1177060:CDS 45.0%
! CTCAAGGCTGGTGATTTTTG+TGG + chr8_1:9400700-9400719 Msa1177060:CDS 45.0%
TGGAGAGGAGCTTCTAACAT+GGG + chr8_1:9400720-9400739 Msa1177060:CDS 45.0%
TGAAGAGGTTGTAGGATCCA+AGG - chr8_1:9400746-9400765 None:intergenic 45.0%
CTCTCATGCATGATGACTTG+AGG + chr8_1:9400821-9400840 Msa1177060:CDS 45.0%
AAGACCTCCAACACACTTTC+AGG + chr8_1:9400881-9400900 Msa1177060:CDS 45.0%
ATTCCTTGCAATGGAGGACA+TGG + chr8_1:9401294-9401313 Msa1177060:CDS 45.0%
TTCCTTGCAATGGAGGACAT+GGG + chr8_1:9401295-9401314 Msa1177060:CDS 45.0%
! GCGTCGATACAGGGAAAAAA+GGG + chr8_1:9401343-9401362 Msa1177060:CDS 45.0%
GAGTCTTCTTCACCAAGCAT+TGG + chr8_1:9401425-9401444 Msa1177060:CDS 45.0%
!! TCTGGTTTCTGAGGCAAAAG+TGG - chr8_1:9401539-9401558 None:intergenic 45.0%
! GTGATGAAATCTGGCTCATC+TGG - chr8_1:9401557-9401576 None:intergenic 45.0%
!!! TTAATTAATTATTTTACAAA+AGG + chr8_1:9397427-9397446 Msa1177060:intron 5.0%
!! AAAATGATAAAATATATAAT+TGG - chr8_1:9401108-9401127 None:intergenic 5.0%
GTCGTTTGACCGACATCGAA+CGG + chr8_1:9397532-9397551 Msa1177060:intron 50.0%
AGAACACTGCACCTAGAAGC+TGG - chr8_1:9398029-9398048 None:intergenic 50.0%
! CATCCCTGTGATTGATGACC+AGG + chr8_1:9398139-9398158 Msa1177060:CDS 50.0%
! GCACCTTCTTCGCGCATTTT+TGG + chr8_1:9398275-9398294 Msa1177060:CDS 50.0%
!! CATTTTTGGAAGGGGAGAGC+CGG + chr8_1:9398289-9398308 Msa1177060:CDS 50.0%
! GGAACTGAGCCTTGTGGAAT+TGG - chr8_1:9398755-9398774 None:intergenic 50.0%
TTAACTGAGACAGCATGGGC+TGG + chr8_1:9398881-9398900 Msa1177060:CDS 50.0%
TGATGCTCTAACGTCTCGTG+TGG + chr8_1:9399266-9399285 Msa1177060:CDS 50.0%
GGTGGGGTTTGCGCAATTTA+AGG + chr8_1:9399334-9399353 Msa1177060:CDS 50.0%
CTCAGAGCCCTTCCTTACTA+AGG - chr8_1:9399823-9399842 None:intergenic 50.0%
CTCAAGACCGCCACAACAAA+TGG + chr8_1:9400646-9400665 Msa1177060:CDS 50.0%
AAGACCGCCACAACAAATGG+TGG + chr8_1:9400649-9400668 Msa1177060:CDS 50.0%
! GGCTGGTGATTTTTGTGGAG+AGG + chr8_1:9400705-9400724 Msa1177060:CDS 50.0%
GTGGAGAGGAGCTTCTAACA+TGG + chr8_1:9400719-9400738 Msa1177060:CDS 50.0%
! CTTGCCTGAAAGTGTGTTGG+AGG - chr8_1:9400888-9400907 None:intergenic 50.0%
CTCCAACACACTTTCAGGCA+AGG + chr8_1:9400886-9400905 Msa1177060:CDS 50.0%
! AGCCTTGCCTGAAAGTGTGT+TGG - chr8_1:9400891-9400910 None:intergenic 50.0%
CAGTTACGCCTCCAATCCAA+AGG - chr8_1:9401016-9401035 None:intergenic 50.0%
TCCTTGCAATGGAGGACATG+GGG + chr8_1:9401296-9401315 Msa1177060:CDS 50.0%
!! GGCGTCGATACAGGGAAAAA+AGG + chr8_1:9401342-9401361 Msa1177060:CDS 50.0%
! TCTGGCTCATCTGGTTTCTG+AGG - chr8_1:9401548-9401567 None:intergenic 50.0%
GTGAAGCCAACCCTTCTCTC+AGG + chr8_1:9396924-9396943 Msa1177060:intron 55.0%
TGAGTGCAATCGGTGACTGC+AGG - chr8_1:9397489-9397508 None:intergenic 55.0%
TCACGTAGTCAGCACATCGG+TGG + chr8_1:9397510-9397529 Msa1177060:intron 55.0%
GAATCGTCCGCAGCTCAGAT+AGG - chr8_1:9399145-9399164 None:intergenic 55.0%
TGCTCTAACGTCTCGTGTGG+TGG + chr8_1:9399269-9399288 Msa1177060:CDS 55.0%
GATGTGCCACCGTATGCTAC+CGG + chr8_1:9400253-9400272 Msa1177060:CDS 55.0%
AGTGATCCGGTAGCATACGG+TGG - chr8_1:9400262-9400281 None:intergenic 55.0%
AACTCGACGGAACTCAAGGC+TGG + chr8_1:9400688-9400707 Msa1177060:CDS 55.0%
GGAGCTTCTAACATGGGCCT+TGG + chr8_1:9400726-9400745 Msa1177060:CDS 55.0%
TCCCCATGTCCTCCATTGCA+AGG - chr8_1:9401300-9401319 None:intergenic 55.0%
AATGGAGGACATGGGGAGCG+CGG + chr8_1:9401303-9401322 Msa1177060:CDS 60.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 9396651 9401932 9396651 ID=Msa1177060;Name=Msa1177060
chr8_1 mRNA 9396651 9401932 9396651 ID=Msa1177060-mRNA-1;Parent=Msa1177060;Name=Msa1177060-mRNA-1;_AED=0.32;_eAED=0.32;_QI=174|0.87|0.88|1|0.87|0.77|9|343|739
chr8_1 exon 9396651 9396774 9396651 ID=Msa1177060-mRNA-1:exon:3184;Parent=Msa1177060-mRNA-1
chr8_1 exon 9397213 9397291 9397213 ID=Msa1177060-mRNA-1:exon:3185;Parent=Msa1177060-mRNA-1
chr8_1 exon 9397896 9398394 9397896 ID=Msa1177060-mRNA-1:exon:3186;Parent=Msa1177060-mRNA-1
chr8_1 exon 9398731 9398943 9398731 ID=Msa1177060-mRNA-1:exon:3187;Parent=Msa1177060-mRNA-1
chr8_1 exon 9399051 9399355 9399051 ID=Msa1177060-mRNA-1:exon:3188;Parent=Msa1177060-mRNA-1
chr8_1 exon 9399703 9399814 9399703 ID=Msa1177060-mRNA-1:exon:3189;Parent=Msa1177060-mRNA-1
chr8_1 exon 9400185 9400421 9400185 ID=Msa1177060-mRNA-1:exon:3190;Parent=Msa1177060-mRNA-1
chr8_1 exon 9400505 9401028 9400505 ID=Msa1177060-mRNA-1:exon:3191;Parent=Msa1177060-mRNA-1
chr8_1 exon 9401289 9401932 9401289 ID=Msa1177060-mRNA-1:exon:3192;Parent=Msa1177060-mRNA-1
chr8_1 five_prime_UTR 9396651 9396774 9396651 ID=Msa1177060-mRNA-1:five_prime_utr;Parent=Msa1177060-mRNA-1
chr8_1 five_prime_UTR 9397213 9397262 9397213 ID=Msa1177060-mRNA-1:five_prime_utr;Parent=Msa1177060-mRNA-1
chr8_1 CDS 9397263 9397291 9397263 ID=Msa1177060-mRNA-1:cds;Parent=Msa1177060-mRNA-1
chr8_1 CDS 9397896 9398394 9397896 ID=Msa1177060-mRNA-1:cds;Parent=Msa1177060-mRNA-1
chr8_1 CDS 9398731 9398943 9398731 ID=Msa1177060-mRNA-1:cds;Parent=Msa1177060-mRNA-1
chr8_1 CDS 9399051 9399355 9399051 ID=Msa1177060-mRNA-1:cds;Parent=Msa1177060-mRNA-1
chr8_1 CDS 9399703 9399814 9399703 ID=Msa1177060-mRNA-1:cds;Parent=Msa1177060-mRNA-1
chr8_1 CDS 9400185 9400421 9400185 ID=Msa1177060-mRNA-1:cds;Parent=Msa1177060-mRNA-1
chr8_1 CDS 9400505 9401028 9400505 ID=Msa1177060-mRNA-1:cds;Parent=Msa1177060-mRNA-1
chr8_1 CDS 9401289 9401589 9401289 ID=Msa1177060-mRNA-1:cds;Parent=Msa1177060-mRNA-1
chr8_1 three_prime_UTR 9401590 9401932 9401590 ID=Msa1177060-mRNA-1:three_prime_utr;Parent=Msa1177060-mRNA-1
Gene Sequence

>Msa1177060

ATGACTACAAATGAAAAGAAATTTGTGAGATTTGAAGATTGGAAGTCAGAATCATCATCTTTTAATTTTTCACAATATGATTCTTCAATTGATGGATTTGAAAAAAGAAAATCTAAACCAAGTGTTACTCCAGCAAAAAATAATGAATCATTGGAAAAACCAGCTTCTAGGTGCAGTGTTCTTGATCCACAAGGTCCACTACTTCAGAAATGGAACAAGATCTTTGTTATAACATGTGTGATGGCAGTATCTATGGATCCATTATTCTTTTACATCCCTGTGATTGATGACCAGGGAAAATGCCTTAATTTGAATGGAACATTGAAGATTACTGCTAGTGTTCTTCGCACGTTTTTCGATCTTTTCTACATTATTCGCATCGTCTTTCAGTTTCGAACCGGGTTTATTGCACCTTCTTCGCGCATTTTTGGAAGGGGAGAGCCGGTTGACGATCCTTTTGCTATAGCAATGAGATACTTAAGTTCTCACTTCATCATTGACCTTCTATCAATTCTTCCACTTCCTCAGATGGTGATTTTAGCAATGATTCCAATTCCACAAGGCTCAGTTCCATATATAACAAAGGATTGGTTGAAGTATACGATAATAACACAGTACGCGCCAAGACTTATGCGAATCTATACGTTGTTCAATGAAGTAACAAGCACTTCTGGTATATTAACTGAGACAGCATGGGCTGGAGCTGCTTATAATCTCTTTCTTTATATGCTAGCAAGTCATGTTGTTGGAGCATTTTGGTATTTGTTTTCGATAGAATCAGAGTTGCGGTGTTGGCGCAGACGATTGAAGAATACTCCATTCTTTGATGATTCCTATCTGAGCTGCGGACGATTCAATTCGACGCTTTTTTCAATTCTCAACAACTCTACTACTTGTCCTTACAAAGATCCTGATGATATTATTGGCCCAACAGTTTTCAATTTTGGAATATTTATTGATGCTCTAACGTCTCGTGTGGTGGAGTTGAATACAAACTTTCATCATAAATTCTTCTACTGCTTTTGGTGGGGTTTGCGCAATTTAAGTTCTGTTGGGCAGAACCTGAAAACAAGCACTTATATTGGGGAGATAATCTTTGCAATCTTCATTGCTGTCTTTGGATTGGTTCTATTTGCATTACTTATAGGAAACATGCAGAAATATCTACAATCTACAACTGTTAGAGTTGAAGAGATGAGAATCAAAAGGAGGGATGCAGAACAATGGATGTGCCACCGTATGCTACCGGATCACTTGAAGCAAAGAATTCGACGGTATGAACAATACAAATGGCAAGAAAATCGAGGCGTCGAAGAAGAGAAACTAATTTACAACCTCCCTAAAGATCTTAGAAGAGACATAAAGAGACATCTTTGCTTAGATTTACTTAAAAAAGTGCCAATTTTTGGCAACATGGATAAACAGTTATTGGATGCAATGTGTGATAAACTGAAGCCGGTCCTTTATACCGAGAAAAGTTTCGTTGTTTGTGAAGGTGATCCAGTGGACGAAATGCTCTTCATCATGCGCGGAAAACTCAAGACCGCCACAACAAATGGTGGAAGAACTGGTTTCTTTAACTCGACGGAACTCAAGGCTGGTGATTTTTGTGGAGAGGAGCTTCTAACATGGGCCTTGGATCCTACAACCTCTTCAAACTTACCTATTTCGACTAGTACAGTTGAAACTATTACGGAAGTTGAAGCTTTTGCTCTCATGCATGATGACTTGAGGATCGTTGCTTCCCAATTTCGACGTCTTATTAACAGAAAAGACCTCCAACACACTTTCAGGCAAGGCTTGACATATTTTTTCACCTTAGATAATACATCATTTCGTTTTTGTAGAAAAAATTTACATTATAAGAAACAAGGTACATTGCAGAAGTCATCTAAAAATTTCACCTTTGGATTGGAGGCGTTTTATTCCTTGCAATGGAGGACATGGGGAGCGCGGTTCATACAAGTAATATGGCGTCGATACAGGGAAAAAAGGGCTCAGAAATTGTTACGTGAAACGGAAAAAAGGCAACAAAATGCTTCTGAAAATGAGGAAGAGTCTTCTTCACCAAGCATTGGTTCCACTGTATATGTACAAAGGTTTGCATCCAATGCATTAAAGCACTTGAGAAGTGGCAAAAGAGTGACACAGACAAAGAAATTTCTACCACTTTTGCCTCAGAAACCAGATGAGCCAGATTTCATCACTCAAAAAAATTAA
Protein sequence

>Msa1177060

MTTNEKKFVRFEDWKSESSSFNFSQYDSSIDGFEKRKSKPSVTPAKNNESLEKPASRCSVLDPQGPLLQKWNKIFVITCVMAVSMDPLFFYIPVIDDQGKCLNLNGTLKITASVLRTFFDLFYIIRIVFQFRTGFIAPSSRIFGRGEPVDDPFAIAMRYLSSHFIIDLLSILPLPQMVILAMIPIPQGSVPYITKDWLKYTIITQYAPRLMRIYTLFNEVTSTSGILTETAWAGAAYNLFLYMLASHVVGAFWYLFSIESELRCWRRRLKNTPFFDDSYLSCGRFNSTLFSILNNSTTCPYKDPDDIIGPTVFNFGIFIDALTSRVVELNTNFHHKFFYCFWWGLRNLSSVGQNLKTSTYIGEIIFAIFIAVFGLVLFALLIGNMQKYLQSTTVRVEEMRIKRRDAEQWMCHRMLPDHLKQRIRRYEQYKWQENRGVEEEKLIYNLPKDLRRDIKRHLCLDLLKKVPIFGNMDKQLLDAMCDKLKPVLYTEKSFVVCEGDPVDEMLFIMRGKLKTATTNGGRTGFFNSTELKAGDFCGEELLTWALDPTTSSNLPISTSTVETITEVEAFALMHDDLRIVASQFRRLINRKDLQHTFRQGLTYFFTLDNTSFRFCRKNLHYKKQGTLQKSSKNFTFGLEAFYSLQWRTWGARFIQVIWRRYREKRAQKLLRETEKRQQNASENEEESSSPSIGSTVYVQRFASNALKHLRSGKRVTQTKKFLPLLPQKPDEPDFITQKN*