AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0358420

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 145 sgRNAs with CRISPR-Local

Find 199 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTGTGAAATTGAACTTTA+TGG 0.192348 3_1:+43321073 Msa0358420:three_prime_UTR
CTGAAACTTGTTCATGATTT+GGG 0.195292 3_1:-43320286 None:intergenic
TGTATTTCTTCTGATGTTGT+AGG 0.215421 3_1:+43320941 Msa0358420:three_prime_UTR
CCTGAAACTTGTTCATGATT+TGG 0.219185 3_1:-43320287 None:intergenic
TTTGTTGAGCGTAGGGAATT+TGG 0.221510 3_1:+43320855 Msa0358420:three_prime_UTR
TTGTGAACTTTGTTGTTTAA+AGG 0.226167 3_1:-43318379 None:intergenic
CAATCTTCGTCTCAATGTTT+TGG 0.227735 3_1:+43319650 Msa0358420:three_prime_UTR
CTTCTGATGTTGTAGGTTAC+AGG 0.233561 3_1:+43320948 Msa0358420:three_prime_UTR
CTTTCTCTAGTTTACCATTC+TGG 0.234179 3_1:-43319782 None:intergenic
CCAAATCATGAACAAGTTTC+AGG 0.245310 3_1:+43320287 Msa0358420:three_prime_UTR
TTAATGGTTTCGGAATATTT+TGG 0.254413 3_1:+43320026 Msa0358420:three_prime_UTR
GTCTCTACTAAGACCATTAA+TGG 0.256641 3_1:+43319600 Msa0358420:three_prime_UTR
GATCTGTTTCATTCTCCTTC+AGG 0.261707 3_1:+43319825 Msa0358420:three_prime_UTR
TGGTCTTCGTTTGCAATCTT+AGG 0.278614 3_1:-43320002 None:intergenic
TGCAAACGAAGACCACTTAA+TGG 0.284606 3_1:+43320010 Msa0358420:three_prime_UTR
ATTGACTTCAATGTCCTTAG+AGG 0.297181 3_1:-43319427 None:intergenic
GTTCCTTCCACCTCCATTAA+TGG 0.307297 3_1:-43319613 None:intergenic
TGAGTCATTGGCCAGAGATT+TGG 0.310125 3_1:+43319959 Msa0358420:three_prime_UTR
TGTGAACTTTGTTGTTTAAA+GGG 0.318556 3_1:-43318378 None:intergenic
CATTGACATTTAGATCAAAA+AGG 0.329626 3_1:-43321051 None:intergenic
CAATTACTGTTGTATGATCA+TGG 0.343347 3_1:+43319305 Msa0358420:exon
TTGTTGAGCGTAGGGAATTT+GGG 0.349514 3_1:+43320856 Msa0358420:three_prime_UTR
TGCTGAACCATCTGCGGCTC+TGG 0.358153 3_1:-43320416 None:intergenic
CTTTGAAACAACCGAAGTTC+AGG 0.363435 3_1:+43318493 Msa0358420:CDS
CGGTGCTACACCTGATGTTA+TGG 0.365739 3_1:+43319875 Msa0358420:three_prime_UTR
CGAAGACCACTTAATGGTTT+CGG 0.367450 3_1:+43320016 Msa0358420:three_prime_UTR
TCCTTGGTTTAAGGGAATAC+AGG 0.374155 3_1:+43320632 Msa0358420:three_prime_UTR
TCGTTTGCAATCTTAGGATC+TGG 0.377435 3_1:-43319996 None:intergenic
AAGCAACGGTCCTTCCTCTA+AGG 0.393297 3_1:+43319413 Msa0358420:three_prime_UTR
CCAAGCAGTGTGTCAACAAA+TGG 0.396904 3_1:+43320200 Msa0358420:three_prime_UTR
GAAAGTGAACCAACTACTTC+AGG 0.397702 3_1:+43320518 Msa0358420:three_prime_UTR
GAGAGAAATGAATGATATGT+AGG 0.403699 3_1:-43318324 None:intergenic
CTTGAATTTCCTGAAGTAGT+TGG 0.407339 3_1:-43320527 None:intergenic
CCCTTTAGAACCTCAGTCTT+GGG 0.408315 3_1:+43319351 Msa0358420:three_prime_UTR
CTCGGTCATTAGATGAAGAT+TGG 0.425115 3_1:+43320721 Msa0358420:three_prime_UTR
TTAGTAGAGACACTGTTCTC+TGG 0.427254 3_1:-43319588 None:intergenic
TTCACAGTCACAACAGGCTT+AGG 0.428362 3_1:-43320386 None:intergenic
TCTAAGGACATTGAAGTCAA+TGG 0.431544 3_1:+43319429 Msa0358420:three_prime_UTR
CATGTGTTCTAGTGATAATA+TGG 0.437417 3_1:+43321114 Msa0358420:three_prime_UTR
GTGAGTTATGGATGCTTAGC+AGG 0.442760 3_1:+43319694 Msa0358420:three_prime_UTR
CTCGGAAACATGTGAGTCAT+TGG 0.443554 3_1:+43319947 Msa0358420:three_prime_UTR
TTGTTCATGATTTGGGGACT+TGG 0.443994 3_1:-43320279 None:intergenic
CATCTCTCAAATGTGAGTTA+TGG 0.448295 3_1:+43319682 Msa0358420:three_prime_UTR
TGTTCTCTGGATTACTTGTC+AGG 0.449342 3_1:-43319575 None:intergenic
CCCAAGACTGAGGTTCTAAA+GGG 0.451045 3_1:-43319351 None:intergenic
CAAGCAGTGTGTCAACAAAT+GGG 0.452081 3_1:+43320201 Msa0358420:three_prime_UTR
ATTCATGACTGCGTGACTAC+AGG 0.454594 3_1:-43320698 None:intergenic
CACACATGGGATCTGATCAT+TGG 0.456050 3_1:-43320818 None:intergenic
GCTCGATGTTTATATACATC+AGG 0.456152 3_1:+43319494 Msa0358420:three_prime_UTR
GACTCAGTGCTCTGATAGCT+TGG 0.460570 3_1:+43320064 Msa0358420:three_prime_UTR
TATCTTGAAGATCAGTTGCT+TGG 0.461888 3_1:+43319726 Msa0358420:three_prime_UTR
CTAAGGACATTGAAGTCAAT+GGG 0.464490 3_1:+43319430 Msa0358420:three_prime_UTR
ACCCTTTAGAACCTCAGTCT+TGG 0.466024 3_1:+43319350 Msa0358420:three_prime_UTR
AGGGAAAGGAGGATGGATAT+AGG 0.467871 3_1:-43318359 None:intergenic
TCAATATTCACAGTCACAAC+AGG 0.469854 3_1:-43320392 None:intergenic
ACCCAAGACTGAGGTTCTAA+AGG 0.472225 3_1:-43319352 None:intergenic
AGATCAGAACAATTGTATCT+TGG 0.479517 3_1:-43320791 None:intergenic
GGAAGCTGGTGTTCGTCTTG+TGG 0.480983 3_1:+43320115 Msa0358420:three_prime_UTR
TGATGCTGAAAATCATAGTT+CGG 0.482073 3_1:+43320094 Msa0358420:three_prime_UTR
ATCTGCTATGCCGGGGAAAG+TGG 0.488327 3_1:+43319902 Msa0358420:three_prime_UTR
TGTGCAAGTGGAACCTCCTA+AGG 0.488557 3_1:+43320166 Msa0358420:three_prime_UTR
TATGGCAATATCTGCTATGC+CGG 0.490303 3_1:+43319893 Msa0358420:three_prime_UTR
TAGAACCTCAGTCTTGGGTG+AGG 0.492450 3_1:+43319356 Msa0358420:three_prime_UTR
GGCTTAGGGAATAAATCATT+CGG 0.497719 3_1:-43320371 None:intergenic
CTTGGTGGAGACTCAACCTT+AGG 0.499060 3_1:-43320182 None:intergenic
GCAGATATTGCCATAACATC+AGG 0.499230 3_1:-43319885 None:intergenic
GAGCGTAGGGAATTTGGGTC+CGG 0.500295 3_1:+43320861 Msa0358420:three_prime_UTR
ATGGCAATATCTGCTATGCC+GGG 0.503138 3_1:+43319894 Msa0358420:three_prime_UTR
TGTTTAAAGGGAAAGGAGGA+TGG 0.505064 3_1:-43318366 None:intergenic
TCACAGTCACAACAGGCTTA+GGG 0.508245 3_1:-43320385 None:intergenic
CCATTTGTTGACACACTGCT+TGG 0.508653 3_1:-43320200 None:intergenic
AGAGAAATGAATGATATGTA+GGG 0.511401 3_1:-43318323 None:intergenic
AGAGCCGCAGATGGTTCAGC+AGG 0.512099 3_1:+43320418 Msa0358420:three_prime_UTR
AATATTCCGAAACCATTAAG+TGG 0.512567 3_1:-43320022 None:intergenic
GAAAATCATAGTTCGGAAGC+TGG 0.519872 3_1:+43320101 Msa0358420:three_prime_UTR
AGCGAAAATGAAGCTTCCTA+TGG 0.519913 3_1:+43320490 Msa0358420:three_prime_UTR
GGGTTAGCGTTCTTAGTTTG+CGG 0.519999 3_1:-43320566 None:intergenic
AGGGTGATACAACAGATTGT+GGG 0.523722 3_1:-43319332 None:intergenic
ACTACCTCGGTTCAATCACC+TGG 0.526489 3_1:+43318423 Msa0358420:CDS
AAGGGTGATACAACAGATTG+TGG 0.527553 3_1:-43319333 None:intergenic
ACTAAGACCATTAATGGAGG+TGG 0.529485 3_1:+43319606 Msa0358420:three_prime_UTR
TTCTGATGTTGTAGGTTACA+GGG 0.533539 3_1:+43320949 Msa0358420:three_prime_UTR
ACATGTGTAGCCACTTTCCC+CGG 0.538816 3_1:-43319912 None:intergenic
TTCATGACTGCGTGACTACA+GGG 0.539476 3_1:-43320697 None:intergenic
GGCTACACATGTCACTCAGC+AGG 0.540185 3_1:+43319923 Msa0358420:three_prime_UTR
TTCTTTCTGTAACACTACCT+CGG 0.540301 3_1:+43318410 Msa0358420:CDS
ATGTTTATATACATCAGGCT+AGG 0.540326 3_1:+43319499 Msa0358420:three_prime_UTR
CTCTGCTTCCATAATAGACA+CGG 0.542849 3_1:-43320589 None:intergenic
ACTTTGTTGTTTAAAGGGAA+AGG 0.544154 3_1:-43318373 None:intergenic
GGTGGAGACTCAACCTTAGG+AGG 0.548492 3_1:-43320179 None:intergenic
TGGTTTAAGGGAATACAGGC+AGG 0.549356 3_1:+43320636 Msa0358420:three_prime_UTR
TGAAACTTGTTCATGATTTG+GGG 0.550211 3_1:-43320285 None:intergenic
TTGTTGTTTAAAGGGAAAGG+AGG 0.550854 3_1:-43318370 None:intergenic
AGCACACGGTCACACACACA+TGG 0.553151 3_1:-43320832 None:intergenic
GGTTCACTTTCAATGTCCAT+AGG 0.554954 3_1:-43320506 None:intergenic
TCTGAGCTTTCATGAGCAGA+AGG 0.558176 3_1:-43320233 None:intergenic
CGAAGATTGTCGTTAAACAC+AGG 0.561622 3_1:-43319636 None:intergenic
TTTGTTGACACACTGCTTGG+TGG 0.562603 3_1:-43320197 None:intergenic
TAGCATAAACATCTTGCTTG+TGG 0.568663 3_1:-43319545 None:intergenic
GCCTGTATTCCCTTAAACCA+AGG 0.572851 3_1:-43320633 None:intergenic
GAGCACTCCAACGCATTCCT+CGG 0.573851 3_1:-43319475 None:intergenic
ACTTCAATGTCCTTAGAGGA+AGG 0.576019 3_1:-43319423 None:intergenic
TGTAGCACCGGTGTAAGCAA+TGG 0.580116 3_1:-43319862 None:intergenic
CAATTGTCCATTGCTTACAC+CGG 0.580933 3_1:+43319855 Msa0358420:three_prime_UTR
TGTTTATATACATCAGGCTA+GGG 0.582616 3_1:+43319500 Msa0358420:three_prime_UTR
GGAAGGACCGTTGCTTTGTG+TGG 0.589066 3_1:-43319406 None:intergenic
AGAACCTCAGTCTTGGGTGA+GGG 0.590332 3_1:+43319357 Msa0358420:three_prime_UTR
ACAGAATCAGAGCATGACCC+AGG 0.591826 3_1:-43318441 None:intergenic
CAGTGGTGAGTCTGTGCAAG+TGG 0.593745 3_1:+43320154 Msa0358420:three_prime_UTR
TAGTGTTCACCATGTGAATG+TGG 0.594090 3_1:-43321139 None:intergenic
AACTATCATGTCAAATGCCG+AGG 0.594132 3_1:+43319458 Msa0358420:three_prime_UTR
ACATGTCACTCAGCAGGACT+CGG 0.594812 3_1:+43319929 Msa0358420:three_prime_UTR
TCAAATGCCGAGGAATGCGT+TGG 0.596916 3_1:+43319468 Msa0358420:three_prime_UTR
GGACATTGAAGTCAATGGGA+AGG 0.601234 3_1:+43319434 Msa0358420:three_prime_UTR
CATAACATCAGGTGTAGCAC+CGG 0.601546 3_1:-43319874 None:intergenic
TCAGAAGTGCTAGTCCAGAA+TGG 0.601594 3_1:+43319768 Msa0358420:three_prime_UTR
TCTGCTTCCATAATAGACAC+GGG 0.601885 3_1:-43320588 None:intergenic
GACAATTGAACAAAACCTGA+AGG 0.605270 3_1:-43319840 None:intergenic
TATTGAGCCAGAGCCGCAGA+TGG 0.613301 3_1:+43320409 Msa0358420:three_prime_UTR
ATATCCTGCTGAACCATCTG+CGG 0.615970 3_1:-43320422 None:intergenic
ATCATTCGGAACAACACTTG+AGG 0.622134 3_1:-43320357 None:intergenic
AACATCAGAAGAAATACAGA+AGG 0.623513 3_1:-43320936 None:intergenic
TGGACTAGCACTTCTGACAA+TGG 0.624686 3_1:-43319762 None:intergenic
AGTCACGCAGTCATGAATCT+CGG 0.624964 3_1:+43320703 Msa0358420:three_prime_UTR
CTGCTTCCATAATAGACACG+GGG 0.629788 3_1:-43320587 None:intergenic
GGGTGATACAACAGATTGTG+GGG 0.631149 3_1:-43319331 None:intergenic
AGACCATTAATGGAGGTGGA+AGG 0.648853 3_1:+43319610 Msa0358420:three_prime_UTR
AGTGTTCACCATGTGAATGT+GGG 0.655357 3_1:-43321138 None:intergenic
AATATGGTCCCACATTCACA+TGG 0.659059 3_1:+43321130 Msa0358420:three_prime_UTR
TGGCAATATCTGCTATGCCG+GGG 0.667680 3_1:+43319895 Msa0358420:three_prime_UTR
CTACCTCGGTTCAATCACCT+GGG 0.670426 3_1:+43318424 Msa0358420:CDS
CTACGCTCAACAAAAGCACA+CGG 0.671598 3_1:-43320846 None:intergenic
CTCACCCTCACCCAAGACTG+AGG 0.674424 3_1:-43319361 None:intergenic
TACAGAAGGAGAAACCACAC+TGG 0.675767 3_1:-43320922 None:intergenic
AAGCAGTGTGTCAACAAATG+GGG 0.677994 3_1:+43320202 Msa0358420:three_prime_UTR
GATGTTACCACACAAAGCAA+CGG 0.679929 3_1:+43319399 Msa0358420:three_prime_UTR
GCACACGGTCACACACACAT+GGG 0.680521 3_1:-43320831 None:intergenic
GAAGATTGTCGTTAAACACA+GGG 0.680691 3_1:-43319635 None:intergenic
TCTACTAAGACCATTAATGG+AGG 0.690612 3_1:+43319603 Msa0358420:three_prime_UTR
GAAAGCTTCTCAGCATGCAG+TGG 0.698296 3_1:+43320137 Msa0358420:three_prime_UTR
TGAGTTATGGATGCTTAGCA+GGG 0.699595 3_1:+43319695 Msa0358420:three_prime_UTR
AAATAATGTAGTATCCAGTG+TGG 0.711608 3_1:+43320908 Msa0358420:three_prime_UTR
TGACCCAGGTGATTGAACCG+AGG 0.745081 3_1:-43318427 None:intergenic
TGCTTCCATAATAGACACGG+GGG 0.772529 3_1:-43320586 None:intergenic
ATGATATGTAGGGATCTGTG+AGG 0.817113 3_1:-43318313 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAGATTTTAGATTAAAAACT+GGG + chr3_1:43320665-43320684 Msa0358420:three_prime_UTR 15.0%
!!! ATTTAATTTTGAAGTCAATT+TGG + chr3_1:43320762-43320781 Msa0358420:three_prime_UTR 15.0%
!!! ATTAAAGTTTGTAGCTTTAT+TGG + chr3_1:43318774-43318793 Msa0358420:intron 20.0%
!!! AGTTATGTTAGTTTCTTTTT+TGG + chr3_1:43319048-43319067 Msa0358420:intron 20.0%
!!! AGTTATGTTAGTTTCTTTTT+TGG + chr3_1:43319048-43319067 Msa0358420:intron 20.0%
!!! AATATATGTGTTTTTAGTAC+TGG + chr3_1:43319139-43319158 Msa0358420:intron 20.0%
!!! GTAGATTTTAGATTAAAAAC+TGG + chr3_1:43320664-43320683 Msa0358420:three_prime_UTR 20.0%
! AGAGAAATGAATGATATGTA+GGG - chr3_1:43318326-43318345 None:intergenic 25.0%
!!! TGTGAACTTTGTTGTTTAAA+GGG - chr3_1:43318381-43318400 None:intergenic 25.0%
!!! TTGTGAACTTTGTTGTTTAA+AGG - chr3_1:43318382-43318401 None:intergenic 25.0%
! GTAATAATTGAAGCAAAACT+AGG - chr3_1:43318603-43318622 None:intergenic 25.0%
! ATTTGATGAGGAATTTAAAG+TGG + chr3_1:43318644-43318663 Msa0358420:intron 25.0%
!! TTTGATGAGGAATTTAAAGT+GGG + chr3_1:43318645-43318664 Msa0358420:intron 25.0%
! ACTAGCCAAAATAATCAAAA+GGG - chr3_1:43318822-43318841 None:intergenic 25.0%
! AACTAGCCAAAATAATCAAA+AGG - chr3_1:43318823-43318842 None:intergenic 25.0%
!! TTTGGACAATTCTTGTATTT+TGG + chr3_1:43318850-43318869 Msa0358420:intron 25.0%
!! TTGAAAGTTTCAATTTTGAG+AGG + chr3_1:43318883-43318902 Msa0358420:intron 25.0%
!!! TTCAATTTTGAGAGGTTATT+AGG + chr3_1:43318891-43318910 Msa0358420:intron 25.0%
! ATTAACTGTCCTAAAATCTA+GGG - chr3_1:43319122-43319141 None:intergenic 25.0%
! TATTAACTGTCCTAAAATCT+AGG - chr3_1:43319123-43319142 None:intergenic 25.0%
!! TTTTGTGTTTTGCTAATACT+AGG + chr3_1:43319171-43319190 Msa0358420:intron 25.0%
! GTGTTAAAACTTGTATGAAT+TGG + chr3_1:43319249-43319268 Msa0358420:intron 25.0%
!! TTAATGGTTTCGGAATATTT+TGG + chr3_1:43320026-43320045 Msa0358420:three_prime_UTR 25.0%
! AACCAAAGAAGTAAAAAAAG+TGG + chr3_1:43320310-43320329 Msa0358420:three_prime_UTR 25.0%
! TTAGATTAAAAACTGGGATT+TGG + chr3_1:43320671-43320690 Msa0358420:three_prime_UTR 25.0%
! CATTGACATTTAGATCAAAA+AGG - chr3_1:43321054-43321073 None:intergenic 25.0%
! GATTGTGAAATTGAACTTTA+TGG + chr3_1:43321073-43321092 Msa0358420:three_prime_UTR 25.0%
GAGAGAAATGAATGATATGT+AGG - chr3_1:43318327-43318346 None:intergenic 30.0%
!! ACTTTGTTGTTTAAAGGGAA+AGG - chr3_1:43318376-43318395 None:intergenic 30.0%
!!! ATGCTTTTTTACCTGAACTT+CGG - chr3_1:43318507-43318526 None:intergenic 30.0%
!!! CTTTTTTGTGTGATTTGATG+AGG + chr3_1:43318632-43318651 Msa0358420:intron 30.0%
! AATTTAAAGTGGGTTTGTGT+TGG + chr3_1:43318655-43318674 Msa0358420:intron 30.0%
!! TGTGACCCTTTTGATTATTT+TGG + chr3_1:43318814-43318833 Msa0358420:intron 30.0%
!!! TTTGGCTAGTTTTTAAGCTT+TGG + chr3_1:43318832-43318851 Msa0358420:intron 30.0%
! TTTGAGAGGTTATTAGGTTT+TGG + chr3_1:43318897-43318916 Msa0358420:intron 30.0%
! ATTCTGAATTTGCTTCTCAA+AGG + chr3_1:43318955-43318974 Msa0358420:intron 30.0%
!! TTAGCTAGTCTTAAGAATCT+TGG + chr3_1:43319198-43319217 Msa0358420:intron 30.0%
!! TAGCTAGTCTTAAGAATCTT+GGG + chr3_1:43319199-43319218 Msa0358420:intron 30.0%
CAATTACTGTTGTATGATCA+TGG + chr3_1:43319305-43319324 Msa0358420:exon 30.0%
ATGTTTATATACATCAGGCT+AGG + chr3_1:43319499-43319518 Msa0358420:three_prime_UTR 30.0%
TGTTTATATACATCAGGCTA+GGG + chr3_1:43319500-43319519 Msa0358420:three_prime_UTR 30.0%
! CTCAATTTTATCCAAATCTC+TGG - chr3_1:43319973-43319992 None:intergenic 30.0%
AATATTCCGAAACCATTAAG+TGG - chr3_1:43320025-43320044 None:intergenic 30.0%
TGATGCTGAAAATCATAGTT+CGG + chr3_1:43320094-43320113 Msa0358420:three_prime_UTR 30.0%
! TGAAACTTGTTCATGATTTG+GGG - chr3_1:43320288-43320307 None:intergenic 30.0%
! CTGAAACTTGTTCATGATTT+GGG - chr3_1:43320289-43320308 None:intergenic 30.0%
!!! GTCCACTTTTTTTACTTCTT+TGG - chr3_1:43320315-43320334 None:intergenic 30.0%
AGATCAGAACAATTGTATCT+TGG - chr3_1:43320794-43320813 None:intergenic 30.0%
AAATAATGTAGTATCCAGTG+TGG + chr3_1:43320908-43320927 Msa0358420:three_prime_UTR 30.0%
AACATCAGAAGAAATACAGA+AGG - chr3_1:43320939-43320958 None:intergenic 30.0%
TGTATTTCTTCTGATGTTGT+AGG + chr3_1:43320941-43320960 Msa0358420:three_prime_UTR 30.0%
TCAAAAAGGACAAAGAAAGA+AGG - chr3_1:43321040-43321059 None:intergenic 30.0%
CATGTGTTCTAGTGATAATA+TGG + chr3_1:43321114-43321133 Msa0358420:three_prime_UTR 30.0%
!! TTGTTGTTTAAAGGGAAAGG+AGG - chr3_1:43318373-43318392 None:intergenic 35.0%
TTCTTTCTGTAACACTACCT+CGG + chr3_1:43318410-43318429 Msa0358420:CDS 35.0%
TTGTGTTGGAATTGTGTCAT+AGG + chr3_1:43318669-43318688 Msa0358420:intron 35.0%
AATTGTGTCATAGGTAGCAT+AGG + chr3_1:43318678-43318697 Msa0358420:intron 35.0%
!! TTTTCCTGTTTTTCCTCTCT+TGG + chr3_1:43318718-43318737 Msa0358420:intron 35.0%
! TTGTTCCTCTTGTGTTTTGT+TGG + chr3_1:43318920-43318939 Msa0358420:intron 35.0%
CAAAACCAACAAAACACAAG+AGG - chr3_1:43318928-43318947 None:intergenic 35.0%
! GAATTTGCTTCTCAAAGGTT+TGG + chr3_1:43318960-43318979 Msa0358420:intron 35.0%
! TCTGCTTAACCCTAGATTTT+AGG + chr3_1:43319110-43319129 Msa0358420:intron 35.0%
! ATTGACTTCAATGTCCTTAG+AGG - chr3_1:43319430-43319449 None:intergenic 35.0%
TCTAAGGACATTGAAGTCAA+TGG + chr3_1:43319429-43319448 Msa0358420:three_prime_UTR 35.0%
CTAAGGACATTGAAGTCAAT+GGG + chr3_1:43319430-43319449 Msa0358420:three_prime_UTR 35.0%
GCTCGATGTTTATATACATC+AGG + chr3_1:43319494-43319513 Msa0358420:three_prime_UTR 35.0%
TAGCATAAACATCTTGCTTG+TGG - chr3_1:43319548-43319567 None:intergenic 35.0%
GTCTCTACTAAGACCATTAA+TGG + chr3_1:43319600-43319619 Msa0358420:three_prime_UTR 35.0%
TCTACTAAGACCATTAATGG+AGG + chr3_1:43319603-43319622 Msa0358420:three_prime_UTR 35.0%
GAAGATTGTCGTTAAACACA+GGG - chr3_1:43319638-43319657 None:intergenic 35.0%
! CAATCTTCGTCTCAATGTTT+TGG + chr3_1:43319650-43319669 Msa0358420:three_prime_UTR 35.0%
CATCTCTCAAATGTGAGTTA+TGG + chr3_1:43319682-43319701 Msa0358420:three_prime_UTR 35.0%
TATCTTGAAGATCAGTTGCT+TGG + chr3_1:43319726-43319745 Msa0358420:three_prime_UTR 35.0%
! CTTTCTCTAGTTTACCATTC+TGG - chr3_1:43319785-43319804 None:intergenic 35.0%
GACAATTGAACAAAACCTGA+AGG - chr3_1:43319843-43319862 None:intergenic 35.0%
! CCTGAAACTTGTTCATGATT+TGG - chr3_1:43320290-43320309 None:intergenic 35.0%
CCAAATCATGAACAAGTTTC+AGG + chr3_1:43320287-43320306 Msa0358420:three_prime_UTR 35.0%
AAAAAAGTGGACGTCAAAGA+CGG + chr3_1:43320323-43320342 Msa0358420:three_prime_UTR 35.0%
GGCTTAGGGAATAAATCATT+CGG - chr3_1:43320374-43320393 None:intergenic 35.0%
TCAATATTCACAGTCACAAC+AGG - chr3_1:43320395-43320414 None:intergenic 35.0%
CTTGAATTTCCTGAAGTAGT+TGG - chr3_1:43320530-43320549 None:intergenic 35.0%
! CTTTTGCTTCCTTGGTTTAA+GGG + chr3_1:43320624-43320643 Msa0358420:three_prime_UTR 35.0%
! CACTGCTATTATCAGTTTTC+CGG - chr3_1:43320883-43320902 None:intergenic 35.0%
TTCTGATGTTGTAGGTTACA+GGG + chr3_1:43320949-43320968 Msa0358420:three_prime_UTR 35.0%
ATGATATGTAGGGATCTGTG+AGG - chr3_1:43318316-43318335 None:intergenic 40.0%
TGTTTAAAGGGAAAGGAGGA+TGG - chr3_1:43318369-43318388 None:intergenic 40.0%
!! CTTTGAAACAACCGAAGTTC+AGG + chr3_1:43318493-43318512 Msa0358420:CDS 40.0%
GAATCCAAGAGAGGAAAAAC+AGG - chr3_1:43318725-43318744 None:intergenic 40.0%
! TTTTCCTCTCTTGGATTCTG+TGG + chr3_1:43318727-43318746 Msa0358420:intron 40.0%
TTTGCTTCTCAAAGGTTTGG+AGG + chr3_1:43318963-43318982 Msa0358420:intron 40.0%
!! TGTAGCTGTTTTGCTTTGAC+AGG + chr3_1:43319278-43319297 Msa0358420:intron 40.0%
AGGGTGATACAACAGATTGT+GGG - chr3_1:43319335-43319354 None:intergenic 40.0%
AAGGGTGATACAACAGATTG+TGG - chr3_1:43319336-43319355 None:intergenic 40.0%
GATGTTACCACACAAAGCAA+CGG + chr3_1:43319399-43319418 Msa0358420:three_prime_UTR 40.0%
ACTTCAATGTCCTTAGAGGA+AGG - chr3_1:43319426-43319445 None:intergenic 40.0%
AACTATCATGTCAAATGCCG+AGG + chr3_1:43319458-43319477 Msa0358420:three_prime_UTR 40.0%
TGTTCTCTGGATTACTTGTC+AGG - chr3_1:43319578-43319597 None:intergenic 40.0%
TTAGTAGAGACACTGTTCTC+TGG - chr3_1:43319591-43319610 None:intergenic 40.0%
ACTAAGACCATTAATGGAGG+TGG + chr3_1:43319606-43319625 Msa0358420:three_prime_UTR 40.0%
CGAAGATTGTCGTTAAACAC+AGG - chr3_1:43319639-43319658 None:intergenic 40.0%
TGAGTTATGGATGCTTAGCA+GGG + chr3_1:43319695-43319714 Msa0358420:three_prime_UTR 40.0%
!!! TCAGTTGCTTGGTTTTGCTT+TGG + chr3_1:43319737-43319756 Msa0358420:three_prime_UTR 40.0%
GATCTGTTTCATTCTCCTTC+AGG + chr3_1:43319825-43319844 Msa0358420:three_prime_UTR 40.0%
CAATTGTCCATTGCTTACAC+CGG + chr3_1:43319855-43319874 Msa0358420:three_prime_UTR 40.0%
GCAGATATTGCCATAACATC+AGG - chr3_1:43319888-43319907 None:intergenic 40.0%
TATGGCAATATCTGCTATGC+CGG + chr3_1:43319893-43319912 Msa0358420:three_prime_UTR 40.0%
TCGTTTGCAATCTTAGGATC+TGG - chr3_1:43319999-43320018 None:intergenic 40.0%
TGGTCTTCGTTTGCAATCTT+AGG - chr3_1:43320005-43320024 None:intergenic 40.0%
TGCAAACGAAGACCACTTAA+TGG + chr3_1:43320010-43320029 Msa0358420:three_prime_UTR 40.0%
CGAAGACCACTTAATGGTTT+CGG + chr3_1:43320016-43320035 Msa0358420:three_prime_UTR 40.0%
!!! ATATTTTGGCATTTCGTGCG+AGG + chr3_1:43320040-43320059 Msa0358420:three_prime_UTR 40.0%
GAAAATCATAGTTCGGAAGC+TGG + chr3_1:43320101-43320120 Msa0358420:three_prime_UTR 40.0%
CAAGCAGTGTGTCAACAAAT+GGG + chr3_1:43320201-43320220 Msa0358420:three_prime_UTR 40.0%
AAGCAGTGTGTCAACAAATG+GGG + chr3_1:43320202-43320221 Msa0358420:three_prime_UTR 40.0%
! TTGTTCATGATTTGGGGACT+TGG - chr3_1:43320282-43320301 None:intergenic 40.0%
ATCATTCGGAACAACACTTG+AGG - chr3_1:43320360-43320379 None:intergenic 40.0%
AGCGAAAATGAAGCTTCCTA+TGG + chr3_1:43320490-43320509 Msa0358420:three_prime_UTR 40.0%
GGTTCACTTTCAATGTCCAT+AGG - chr3_1:43320509-43320528 None:intergenic 40.0%
GAAAGTGAACCAACTACTTC+AGG + chr3_1:43320518-43320537 Msa0358420:three_prime_UTR 40.0%
TCTGCTTCCATAATAGACAC+GGG - chr3_1:43320591-43320610 None:intergenic 40.0%
CTCTGCTTCCATAATAGACA+CGG - chr3_1:43320592-43320611 None:intergenic 40.0%
! GCTTTTGCTTCCTTGGTTTA+AGG + chr3_1:43320623-43320642 Msa0358420:three_prime_UTR 40.0%
TCCTTGGTTTAAGGGAATAC+AGG + chr3_1:43320632-43320651 Msa0358420:three_prime_UTR 40.0%
CTCGGTCATTAGATGAAGAT+TGG + chr3_1:43320721-43320740 Msa0358420:three_prime_UTR 40.0%
TTTGTTGAGCGTAGGGAATT+TGG + chr3_1:43320855-43320874 Msa0358420:three_prime_UTR 40.0%
TTGTTGAGCGTAGGGAATTT+GGG + chr3_1:43320856-43320875 Msa0358420:three_prime_UTR 40.0%
CTTCTGATGTTGTAGGTTAC+AGG + chr3_1:43320948-43320967 Msa0358420:three_prime_UTR 40.0%
AATATGGTCCCACATTCACA+TGG + chr3_1:43321130-43321149 Msa0358420:three_prime_UTR 40.0%
AGGGAAAGGAGGATGGATAT+AGG - chr3_1:43318362-43318381 None:intergenic 45.0%
GTCAAAGTTGTGTGTGTGTG+TGG - chr3_1:43318474-43318493 None:intergenic 45.0%
ACTTCCACAGAATCCAAGAG+AGG - chr3_1:43318734-43318753 None:intergenic 45.0%
GGGTGATACAACAGATTGTG+GGG - chr3_1:43319334-43319353 None:intergenic 45.0%
!! ACCCTTTAGAACCTCAGTCT+TGG + chr3_1:43319350-43319369 Msa0358420:three_prime_UTR 45.0%
!! CCCAAGACTGAGGTTCTAAA+GGG - chr3_1:43319354-43319373 None:intergenic 45.0%
!! CCCTTTAGAACCTCAGTCTT+GGG + chr3_1:43319351-43319370 Msa0358420:three_prime_UTR 45.0%
! ACCCAAGACTGAGGTTCTAA+AGG - chr3_1:43319355-43319374 None:intergenic 45.0%
GGACATTGAAGTCAATGGGA+AGG + chr3_1:43319434-43319453 Msa0358420:three_prime_UTR 45.0%
AGACCATTAATGGAGGTGGA+AGG + chr3_1:43319610-43319629 Msa0358420:three_prime_UTR 45.0%
GTTCCTTCCACCTCCATTAA+TGG - chr3_1:43319616-43319635 None:intergenic 45.0%
GTGAGTTATGGATGCTTAGC+AGG + chr3_1:43319694-43319713 Msa0358420:three_prime_UTR 45.0%
!! TGGACTAGCACTTCTGACAA+TGG - chr3_1:43319765-43319784 None:intergenic 45.0%
!! TCAGAAGTGCTAGTCCAGAA+TGG + chr3_1:43319768-43319787 Msa0358420:three_prime_UTR 45.0%
CATAACATCAGGTGTAGCAC+CGG - chr3_1:43319877-43319896 None:intergenic 45.0%
ATGGCAATATCTGCTATGCC+GGG + chr3_1:43319894-43319913 Msa0358420:three_prime_UTR 45.0%
CTCGGAAACATGTGAGTCAT+TGG + chr3_1:43319947-43319966 Msa0358420:three_prime_UTR 45.0%
TGAGTCATTGGCCAGAGATT+TGG + chr3_1:43319959-43319978 Msa0358420:three_prime_UTR 45.0%
TTTGTTGACACACTGCTTGG+TGG - chr3_1:43320200-43320219 None:intergenic 45.0%
! CCATTTGTTGACACACTGCT+TGG - chr3_1:43320203-43320222 None:intergenic 45.0%
CCAAGCAGTGTGTCAACAAA+TGG + chr3_1:43320200-43320219 Msa0358420:three_prime_UTR 45.0%
TCTGAGCTTTCATGAGCAGA+AGG - chr3_1:43320236-43320255 None:intergenic 45.0%
TCACAGTCACAACAGGCTTA+GGG - chr3_1:43320388-43320407 None:intergenic 45.0%
TTCACAGTCACAACAGGCTT+AGG - chr3_1:43320389-43320408 None:intergenic 45.0%
ATATCCTGCTGAACCATCTG+CGG - chr3_1:43320425-43320444 None:intergenic 45.0%
! GGGTTAGCGTTCTTAGTTTG+CGG - chr3_1:43320569-43320588 None:intergenic 45.0%
TGCTTCCATAATAGACACGG+GGG - chr3_1:43320589-43320608 None:intergenic 45.0%
CTGCTTCCATAATAGACACG+GGG - chr3_1:43320590-43320609 None:intergenic 45.0%
! CTTCTGAGCTTTTGCTTCCT+TGG + chr3_1:43320616-43320635 Msa0358420:three_prime_UTR 45.0%
GCCTGTATTCCCTTAAACCA+AGG - chr3_1:43320636-43320655 None:intergenic 45.0%
TGGTTTAAGGGAATACAGGC+AGG + chr3_1:43320636-43320655 Msa0358420:three_prime_UTR 45.0%
TTCATGACTGCGTGACTACA+GGG - chr3_1:43320700-43320719 None:intergenic 45.0%
ATTCATGACTGCGTGACTAC+AGG - chr3_1:43320701-43320720 None:intergenic 45.0%
AGTCACGCAGTCATGAATCT+CGG + chr3_1:43320703-43320722 Msa0358420:three_prime_UTR 45.0%
CACACATGGGATCTGATCAT+TGG - chr3_1:43320821-43320840 None:intergenic 45.0%
! CTACGCTCAACAAAAGCACA+CGG - chr3_1:43320849-43320868 None:intergenic 45.0%
! GTGTGCTTTTGTTGAGCGTA+GGG + chr3_1:43320848-43320867 Msa0358420:three_prime_UTR 45.0%
TACAGAAGGAGAAACCACAC+TGG - chr3_1:43320925-43320944 None:intergenic 45.0%
ACTACCTCGGTTCAATCACC+TGG + chr3_1:43318423-43318442 Msa0358420:CDS 50.0%
CTACCTCGGTTCAATCACCT+GGG + chr3_1:43318424-43318443 Msa0358420:CDS 50.0%
ACAGAATCAGAGCATGACCC+AGG - chr3_1:43318444-43318463 None:intergenic 50.0%
TAGAACCTCAGTCTTGGGTG+AGG + chr3_1:43319356-43319375 Msa0358420:three_prime_UTR 50.0%
AGAACCTCAGTCTTGGGTGA+GGG + chr3_1:43319357-43319376 Msa0358420:three_prime_UTR 50.0%
AAGCAACGGTCCTTCCTCTA+AGG + chr3_1:43319413-43319432 Msa0358420:three_prime_UTR 50.0%
TCAAATGCCGAGGAATGCGT+TGG + chr3_1:43319468-43319487 Msa0358420:three_prime_UTR 50.0%
!! TGTAGCACCGGTGTAAGCAA+TGG - chr3_1:43319865-43319884 None:intergenic 50.0%
!! CGGTGCTACACCTGATGTTA+TGG + chr3_1:43319875-43319894 Msa0358420:three_prime_UTR 50.0%
TGGCAATATCTGCTATGCCG+GGG + chr3_1:43319895-43319914 Msa0358420:three_prime_UTR 50.0%
! ACATGTGTAGCCACTTTCCC+CGG - chr3_1:43319915-43319934 None:intergenic 50.0%
ACATGTCACTCAGCAGGACT+CGG + chr3_1:43319929-43319948 Msa0358420:three_prime_UTR 50.0%
GACTCAGTGCTCTGATAGCT+TGG + chr3_1:43320064-43320083 Msa0358420:three_prime_UTR 50.0%
GAAAGCTTCTCAGCATGCAG+TGG + chr3_1:43320137-43320156 Msa0358420:three_prime_UTR 50.0%
TGTGCAAGTGGAACCTCCTA+AGG + chr3_1:43320166-43320185 Msa0358420:three_prime_UTR 50.0%
CTTGGTGGAGACTCAACCTT+AGG - chr3_1:43320185-43320204 None:intergenic 50.0%
GCTAACCCCCGTGTCTATTA+TGG + chr3_1:43320581-43320600 Msa0358420:three_prime_UTR 50.0%
! CGTGTGCTTTTGTTGAGCGT+AGG + chr3_1:43320847-43320866 Msa0358420:three_prime_UTR 50.0%
TGACCCAGGTGATTGAACCG+AGG - chr3_1:43318430-43318449 None:intergenic 55.0%
! GGAAGGACCGTTGCTTTGTG+TGG - chr3_1:43319409-43319428 None:intergenic 55.0%
GAGCACTCCAACGCATTCCT+CGG - chr3_1:43319478-43319497 None:intergenic 55.0%
ATCTGCTATGCCGGGGAAAG+TGG + chr3_1:43319902-43319921 Msa0358420:three_prime_UTR 55.0%
GGCTACACATGTCACTCAGC+AGG + chr3_1:43319923-43319942 Msa0358420:three_prime_UTR 55.0%
GGAAGCTGGTGTTCGTCTTG+TGG + chr3_1:43320115-43320134 Msa0358420:three_prime_UTR 55.0%
CAGTGGTGAGTCTGTGCAAG+TGG + chr3_1:43320154-43320173 Msa0358420:three_prime_UTR 55.0%
GGTGGAGACTCAACCTTAGG+AGG - chr3_1:43320182-43320201 None:intergenic 55.0%
TATTGAGCCAGAGCCGCAGA+TGG + chr3_1:43320409-43320428 Msa0358420:three_prime_UTR 55.0%
GCACACGGTCACACACACAT+GGG - chr3_1:43320834-43320853 None:intergenic 55.0%
AGCACACGGTCACACACACA+TGG - chr3_1:43320835-43320854 None:intergenic 55.0%
GAGCGTAGGGAATTTGGGTC+CGG + chr3_1:43320861-43320880 Msa0358420:three_prime_UTR 55.0%
CTCACCCTCACCCAAGACTG+AGG - chr3_1:43319364-43319383 None:intergenic 60.0%
TGCTGAACCATCTGCGGCTC+TGG - chr3_1:43320419-43320438 None:intergenic 60.0%
AGAGCCGCAGATGGTTCAGC+AGG + chr3_1:43320418-43320437 Msa0358420:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 43318294 43321159 43318294 ID=Msa0358420;Name=Msa0358420
chr3_1 mRNA 43318294 43321159 43318294 ID=Msa0358420-mRNA-1;Parent=Msa0358420;Name=Msa0358420-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0|1|0.5|1|0|1|2|1838|80
chr3_1 exon 43318294 43318514 43318294 ID=Msa0358420-mRNA-1:exon:7696;Parent=Msa0358420-mRNA-1
chr3_1 exon 43319300 43321159 43319300 ID=Msa0358420-mRNA-1:exon:7697;Parent=Msa0358420-mRNA-1
chr3_1 CDS 43318294 43318514 43318294 ID=Msa0358420-mRNA-1:cds;Parent=Msa0358420-mRNA-1
chr3_1 CDS 43319300 43319321 43319300 ID=Msa0358420-mRNA-1:cds;Parent=Msa0358420-mRNA-1
chr3_1 three_prime_UTR 43319322 43321159 43319322 ID=Msa0358420-mRNA-1:three_prime_utr;Parent=Msa0358420-mRNA-1
Gene Sequence

>Msa0358420

TTTCTTGTATATGTAATTTCCTCACAGATCCCTACATATCATTCATTTCTCTCTCTCATCAATCACCTATATCCATCCTCCTTTCCCTTTAAACAACAAAGTTCACAACTTTTTTGTTCTTTCTGTAACACTACCTCGGTTCAATCACCTGGGTCATGCTCTGATTCTGTCTTTGAGCCACACACACACACAACTTTGACTTTGAAACAACCGAAGTTCAGGTTCTCAATTACTGTTGTATGA
Protein sequence

>Msa0358420

FLVYVISSQIPTYHSFLSLINHLYPSSFPFKQQSSQLFCSFCNTTSVQSPGSCSDSVFEPHTHTTLTLKQPKFRFSITVV*