AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0372110

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0372110 MtrunA17_Chr1g0209681 77.372 137 7 2 1 114 1 136 2.90e-60 193
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0372110 AT1G57600.1 46.479 142 52 2 1 119 1 141 7.83e-35 126
Msa0372110 AT1G57600.2 46.479 142 52 2 1 119 1 141 7.83e-35 126
Msa0372110 AT1G57600.3 46.479 142 52 2 1 119 1 141 7.83e-35 126

Find 103 sgRNAs with CRISPR-Local

Find 183 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTTGTGTGTTATTTATTT+AGG 0.200548 3_1:+70424372 Msa0372110:three_prime_UTR
CTTTGGAATCTTTACACTTC+TGG 0.211962 3_1:+70423067 Msa0372110:CDS
TGGTTGGTCAACAAATTAAA+AGG 0.250958 3_1:+70423854 Msa0372110:three_prime_UTR
AAAGAACTCTTTCTGCTTCC+AGG 0.261759 3_1:-70422640 None:intergenic
CTCTCTTGCGATTCCACGAT+TGG 0.265352 3_1:+70422523 Msa0372110:five_prime_UTR
CCTGTTCTTACACTTGTCTT+TGG 0.273288 3_1:+70423050 Msa0372110:CDS
GCTTAGTCTGAGATTAGTTT+TGG 0.281512 3_1:+70424704 Msa0372110:three_prime_UTR
TTCTTCTTCCTTCGCGAATT+CGG 0.299784 3_1:-70421967 None:intergenic
TATCCCCTTGAGACAATCTA+TGG 0.313160 3_1:-70424441 None:intergenic
CAGAAGCACAAAGCTGAGTT+TGG 0.350409 3_1:+70421899 Msa0372110:five_prime_UTR
TATTGTAACTTTGGATTTGT+TGG 0.353906 3_1:+70424979 Msa0372110:three_prime_UTR
GCATCTGAGGGGAAACTAAA+TGG 0.355710 3_1:+70423834 Msa0372110:exon
GAAGAAAGCCCTCATCAAAT+TGG 0.359772 3_1:-70423090 None:intergenic
AATCTCAGACTAAGCTGAAT+TGG 0.361937 3_1:-70424696 None:intergenic
ATTCGGTTACCTTTGCTGTT+TGG 0.369402 3_1:-70421950 None:intergenic
TCAGCTTTGTGCTTCTGACT+TGG 0.372127 3_1:-70421893 None:intergenic
AAAATGTAATTAAAGGAGAT+AGG 0.375459 3_1:+70424652 Msa0372110:three_prime_UTR
GAAAAGGGAAAAGGGAAAAG+TGG 0.376759 3_1:-70421694 None:intergenic
GAGAGATCAGACTTCTTAGT+TGG 0.380941 3_1:-70422447 None:intergenic
TCTCTTGCGATTCCACGATT+GGG 0.383180 3_1:+70422524 Msa0372110:five_prime_UTR
TACAAAATCATAGAATTATA+AGG 0.389269 3_1:-70424915 None:intergenic
CAGCTTTGTGCTTCTGACTT+GGG 0.392786 3_1:-70421892 None:intergenic
GAAGGGGAGAAAAGGGAAAA+GGG 0.395097 3_1:-70421722 None:intergenic
AAATAACACACAAATTCATT+AGG 0.412866 3_1:-70424365 None:intergenic
CTAATCAGCAACTCAATTCA+AGG 0.416062 3_1:-70422568 None:intergenic
TGAAGGGGAGAAAAGGGAAA+AGG 0.420446 3_1:-70421723 None:intergenic
TCGATTGAGAACGGTTGGTT+TGG 0.423465 3_1:-70421759 None:intergenic
AGGGAAAGTGAAAAGGGAAA+AGG 0.433908 3_1:-70421703 None:intergenic
TTGGATATTGTAACAATGAC+AGG 0.433916 3_1:+70424998 Msa0372110:three_prime_UTR
ACAATTTAATGAGAAGTGAT+AGG 0.441583 3_1:+70424572 Msa0372110:three_prime_UTR
AGGGAAAAGGGAAAGTGAAA+AGG 0.448159 3_1:-70421710 None:intergenic
GGGAAAGTGAAAAGGGAAAA+GGG 0.449050 3_1:-70421702 None:intergenic
TCACGAGTGAAGGGGAGAAA+AGG 0.466417 3_1:-70421730 None:intergenic
GGGAAAAGGGAAAGTGAAAA+GGG 0.467752 3_1:-70421709 None:intergenic
GCCTACCTATCTTATTTGCA+TGG 0.471564 3_1:+70423161 Msa0372110:CDS
CCTCTGTTCTTCCACGAAAA+CGG 0.471630 3_1:-70422084 None:intergenic
AAGTATCTGAACTTTGAAGT+AGG 0.473094 3_1:+70424104 Msa0372110:three_prime_UTR
ATGCTCCATGCAAATAAGAT+AGG 0.474131 3_1:-70423166 None:intergenic
TTGGGAGATCGAAGGAAGAC+AGG 0.474221 3_1:-70422428 None:intergenic
TGAGAGGAATGTCCGTTGTC+TGG 0.481624 3_1:+70423123 Msa0372110:CDS
AGAGATCAGACTTCTTAGTT+GGG 0.483449 3_1:-70422446 None:intergenic
TTGGAGAGTTTCACGAGTGA+AGG 0.487075 3_1:-70421740 None:intergenic
AGACTTGGTGAAGGTTGAAT+CGG 0.487863 3_1:-70421805 None:intergenic
AATAGACTAAGTTGTGAGAC+TGG 0.497263 3_1:+70424727 Msa0372110:three_prime_UTR
CCAAAGACAAGTGTAAGAAC+AGG 0.498674 3_1:-70423050 None:intergenic
AGTATCTGAACTTTGAAGTA+GGG 0.499224 3_1:+70424105 Msa0372110:three_prime_UTR
AGGAGCTAGGCTAAACCATC+AGG 0.501207 3_1:+70424592 Msa0372110:three_prime_UTR
CTGTACTCAAACCTGGAAAA+CGG 0.505341 3_1:+70422486 Msa0372110:five_prime_UTR
AAGAACTCTTTCTGCTTCCA+GGG 0.506003 3_1:-70422639 None:intergenic
TTCTCATTAAATTGCCGAGA+TGG 0.510011 3_1:-70424539 None:intergenic
ATTGTTCGATTGAGAACGGT+TGG 0.512641 3_1:-70421764 None:intergenic
AAAAGGGAAAAGGGAAAAGT+GGG 0.520337 3_1:-70421693 None:intergenic
TGACTTGGGTTTGATATGAA+CGG 0.521217 3_1:-70421878 None:intergenic
AAATGTAATTAAAGGAGATA+GGG 0.523741 3_1:+70424653 Msa0372110:three_prime_UTR
TTCTCATTAAATTGTCGAGA+TGG 0.524264 3_1:-70424564 None:intergenic
CACTTCTGGCCAATTTGATG+AGG 0.526184 3_1:+70423081 Msa0372110:CDS
CTCTTGCGATTCCACGATTG+GGG 0.530968 3_1:+70422525 Msa0372110:five_prime_UTR
TTCTTCAATTTCAATGTGAG+AGG 0.534160 3_1:+70423107 Msa0372110:CDS
AACATAGCCTATGCACTGCC+AGG 0.534676 3_1:-70424880 None:intergenic
AAGAAATTCCACCTCCATCT+CGG 0.534799 3_1:+70424525 Msa0372110:three_prime_UTR
CATCATCAGGCGCTCCCTTG+CGG 0.543631 3_1:+70422698 Msa0372110:CDS
TGGAGAGTTTCACGAGTGAA+GGG 0.546915 3_1:-70421739 None:intergenic
CACGAGTGAAGGGGAGAAAA+GGG 0.548165 3_1:-70421729 None:intergenic
TTAAATTGCCGAGATGGAGG+TGG 0.549539 3_1:-70424533 None:intergenic
CTCTTAGCCTGGCAGTGCAT+AGG 0.552112 3_1:+70424873 Msa0372110:three_prime_UTR
TCTGATGTATAAGCTCCTGA+TGG 0.558906 3_1:-70424607 None:intergenic
ACTTCTGGCCAATTTGATGA+GGG 0.561165 3_1:+70423082 Msa0372110:CDS
ATAAATTGTTCGATTGAGAA+CGG 0.563409 3_1:-70421768 None:intergenic
AGGGAAAAGGGAAAAGTGGG+AGG 0.567293 3_1:-70421690 None:intergenic
CTGAGGGGAAACTAAATGGT+TGG 0.568184 3_1:+70423838 Msa0372110:exon
ACAGTATCCGACAGCATCTG+AGG 0.568896 3_1:+70423821 Msa0372110:CDS
AATGAGAAGTGATAGGAGCT+AGG 0.574062 3_1:+70424579 Msa0372110:three_prime_UTR
TATCTTATTTGCATGGAGCA+TGG 0.574992 3_1:+70423168 Msa0372110:CDS
GCAGCTCGAATCCGAAGAAG+AGG 0.577668 3_1:+70422049 Msa0372110:five_prime_UTR
TCATTAAATTGCCGAGATGG+AGG 0.583358 3_1:-70424536 None:intergenic
TCGTGGAATCGCAAGAGAGA+AGG 0.584250 3_1:-70422519 None:intergenic
TCAACAACTGTACTCAAACC+TGG 0.585004 3_1:+70422479 Msa0372110:five_prime_UTR
TACTCCCATAGATTGTCTCA+AGG 0.588702 3_1:+70424436 Msa0372110:three_prime_UTR
TGTGAATAGCGCCCCAATCG+TGG 0.589285 3_1:-70422536 None:intergenic
CAGTATCCGACAGCATCTGA+GGG 0.590060 3_1:+70423822 Msa0372110:CDS
AGTTTCCCCTCAGATGCTGT+CGG 0.590938 3_1:-70423828 None:intergenic
GGAGAGTTTCACGAGTGAAG+GGG 0.598071 3_1:-70421738 None:intergenic
ACTCCCATAGATTGTCTCAA+GGG 0.598235 3_1:+70424437 Msa0372110:three_prime_UTR
CTTCTTAGTTGGGAGATCGA+AGG 0.610786 3_1:-70422436 None:intergenic
TCAAACCTGGAAAACGGACG+CGG 0.612446 3_1:+70422492 Msa0372110:five_prime_UTR
CTCCCATAGATTGTCTCAAG+GGG 0.617721 3_1:+70424438 Msa0372110:three_prime_UTR
GGCGAAAAGAACGAAGACAT+AGG 0.621764 3_1:+70424393 Msa0372110:three_prime_UTR
ATCCCCTTGAGACAATCTAT+GGG 0.622438 3_1:-70424440 None:intergenic
CAACAACAAACACAAGCTGT+CGG 0.626988 3_1:+70422024 Msa0372110:five_prime_UTR
ATGTCTCAGATGCTCAGTGG+AGG 0.637896 3_1:+70423009 Msa0372110:CDS
AAAGGTAACCGAATTCGCGA+AGG 0.643245 3_1:+70421959 Msa0372110:five_prime_UTR
TGAATCGAACCAAACAGCAA+AGG 0.643380 3_1:+70421941 Msa0372110:five_prime_UTR
GGGAAAAGGGAAAAGTGGGA+GGG 0.644660 3_1:-70421689 None:intergenic
GTTGAAATCAGACTTGGTGA+AGG 0.644985 3_1:-70421814 None:intergenic
GATGTTGTTGAAATCAGACT+TGG 0.654774 3_1:-70421820 None:intergenic
ACAGTACATTGCGATGAACA+AGG 0.655075 3_1:+70422596 Msa0372110:exon
AAAGAAGAAACATACCGCAA+GGG 0.662935 3_1:-70422712 None:intergenic
AGAAAACAAAAGCCAGACAA+CGG 0.663004 3_1:-70423135 None:intergenic
TCCATGCAAATAAGATAGGT+AGG 0.665647 3_1:-70423162 None:intergenic
ACTATAAGAAGAGTGCACCC+TGG 0.671406 3_1:+70422622 Msa0372110:CDS
AGGATGTCTCAGATGCTCAG+TGG 0.679281 3_1:+70423006 Msa0372110:intron
ATGCTAAATACACTTGAACA+CGG 0.697896 3_1:+70424155 Msa0372110:three_prime_UTR
AGTATCCGACAGCATCTGAG+GGG 0.723113 3_1:+70423823 Msa0372110:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAAAATAAAACTTAAATA+TGG - chr3_1:70423941-70423960 None:intergenic 10.0%
!!! ATTTGTTTTTTATTTATCGT+CGG + chr3_1:70422796-70422815 Msa0372110:intron 15.0%
!!! TATTAATTTTCTTCTTGTTA+AGG + chr3_1:70423305-70423324 Msa0372110:CDS 15.0%
!! AAACAATGAAAATTAATCTT+TGG - chr3_1:70423669-70423688 None:intergenic 15.0%
!!! AAAGATTAATTTTCATTGTT+TGG + chr3_1:70423668-70423687 Msa0372110:intron 15.0%
!!! ATTTTAAAACAAGTTTTAAG+TGG - chr3_1:70424042-70424061 None:intergenic 15.0%
!!! AATTTGTGTGTTATTTATTT+AGG + chr3_1:70424372-70424391 Msa0372110:three_prime_UTR 15.0%
!! TGCAAAAAAAATGTAATTAA+AGG + chr3_1:70424645-70424664 Msa0372110:three_prime_UTR 15.0%
!! TACAAAATCATAGAATTATA+AGG - chr3_1:70424918-70424937 None:intergenic 15.0%
!!! ATTTTATGTTATTGTAACTT+TGG + chr3_1:70424970-70424989 Msa0372110:three_prime_UTR 15.0%
!!! TGATTTTGTTTCATAGATTT+AGG + chr3_1:70422118-70422137 Msa0372110:intron 20.0%
!!! ATTTTCTTCAAAGTTTTTCA+CGG - chr3_1:70422211-70422230 None:intergenic 20.0%
!!! AATATTTCTATGTAATGTGT+TGG + chr3_1:70423363-70423382 Msa0372110:intron 20.0%
!! GATATAGAAATCTAGTTAAA+AGG + chr3_1:70423462-70423481 Msa0372110:intron 20.0%
!! AAACAATGAAAATTCAACTA+TGG - chr3_1:70423750-70423769 None:intergenic 20.0%
!!! ATAGTTGAATTTTCATTGTT+TGG + chr3_1:70423749-70423768 Msa0372110:intron 20.0%
!!! ATTAGAAACTTATTTTGTCA+TGG + chr3_1:70424075-70424094 Msa0372110:intron 20.0%
!! AAATAACACACAAATTCATT+AGG - chr3_1:70424368-70424387 None:intergenic 20.0%
!! AAAATGTAATTAAAGGAGAT+AGG + chr3_1:70424652-70424671 Msa0372110:three_prime_UTR 20.0%
!! AAATGTAATTAAAGGAGATA+GGG + chr3_1:70424653-70424672 Msa0372110:three_prime_UTR 20.0%
!!! TTTTTTGTTTTGTTTTAGTG+TGG + chr3_1:70424801-70424820 Msa0372110:three_prime_UTR 20.0%
! ATAAATTGTTCGATTGAGAA+CGG - chr3_1:70421771-70421790 None:intergenic 25.0%
!! AAAATAAGAGTTTGAAACTC+CGG + chr3_1:70422226-70422245 Msa0372110:intron 25.0%
! GCAAAAAAGGAAGAAAAAAA+TGG + chr3_1:70422392-70422411 Msa0372110:five_prime_UTR 25.0%
!! TTTTCTATGTTATCATCATC+AGG + chr3_1:70422685-70422704 Msa0372110:CDS 25.0%
! CTGAAATACTTACAAAGAAA+TGG - chr3_1:70422916-70422935 None:intergenic 25.0%
! TTACACATAACAAACAGAAT+AGG - chr3_1:70422986-70423005 None:intergenic 25.0%
!!! ATTCTGTTTGTTATGTGTAA+AGG + chr3_1:70422986-70423005 Msa0372110:intron 25.0%
! GTTTGTGACAAAAATAACAT+TGG - chr3_1:70423565-70423584 None:intergenic 25.0%
! TTAATCTTTGGCATTTGTTT+CGG - chr3_1:70423657-70423676 None:intergenic 25.0%
!! TTATTGACACGTTTTTCTTT+TGG + chr3_1:70423700-70423719 Msa0372110:intron 25.0%
!! TTATTGACATGTTCTTCTTT+TGG + chr3_1:70423781-70423800 Msa0372110:intron 25.0%
!! AATATTTATCTTTCAAGTCC+TGG + chr3_1:70423907-70423926 Msa0372110:intron 25.0%
! ATAAAACTTAAATATGGCAC+AGG - chr3_1:70423935-70423954 None:intergenic 25.0%
! ACAATTTAATGAGAAGTGAT+AGG + chr3_1:70424572-70424591 Msa0372110:three_prime_UTR 25.0%
! AAACAAAAAAACTCAGAACA+AGG - chr3_1:70424791-70424810 None:intergenic 25.0%
!!! GGTTGTAGTTCTTTTATTTT+AGG + chr3_1:70424822-70424841 Msa0372110:three_prime_UTR 25.0%
! TATTGTAACTTTGGATTTGT+TGG + chr3_1:70424979-70424998 Msa0372110:three_prime_UTR 25.0%
! TCTATTCTTTTCCTCTTCTT+CGG - chr3_1:70422063-70422082 None:intergenic 30.0%
GAAATGAGTTTAGAAAGTGA+AGG + chr3_1:70422175-70422194 Msa0372110:intron 30.0%
CTCGTTGGATAAATTTACAT+TGG + chr3_1:70422291-70422310 Msa0372110:intron 30.0%
CTACATAAACAGGTTATACA+TGG - chr3_1:70422745-70422764 None:intergenic 30.0%
GTCATATCATCTACATAAAC+AGG - chr3_1:70422755-70422774 None:intergenic 30.0%
GATCACTACAAACAACTATT+CGG + chr3_1:70422824-70422843 Msa0372110:intron 30.0%
AATCATACATTGAGGTGATT+AGG - chr3_1:70422872-70422891 None:intergenic 30.0%
TTCTTCAATTTCAATGTGAG+AGG + chr3_1:70423107-70423126 Msa0372110:CDS 30.0%
TTATCTCTAACAGCAAGATA+AGG - chr3_1:70423587-70423606 None:intergenic 30.0%
TCTAAATTGAACAAGTGAGA+AGG - chr3_1:70423620-70423639 None:intergenic 30.0%
! TGGTTGGTCAACAAATTAAA+AGG + chr3_1:70423854-70423873 Msa0372110:three_prime_UTR 30.0%
TTCGAGTGACAAATGAAAAT+AGG - chr3_1:70423889-70423908 None:intergenic 30.0%
! CATGAATGTTTGGAATTACA+AGG - chr3_1:70423968-70423987 None:intergenic 30.0%
TGACAACTTTCATGAATGTT+TGG - chr3_1:70423978-70423997 None:intergenic 30.0%
!! AAGTATCTGAACTTTGAAGT+AGG + chr3_1:70424104-70424123 Msa0372110:three_prime_UTR 30.0%
!! AGTATCTGAACTTTGAAGTA+GGG + chr3_1:70424105-70424124 Msa0372110:three_prime_UTR 30.0%
ATGCTAAATACACTTGAACA+CGG + chr3_1:70424155-70424174 Msa0372110:three_prime_UTR 30.0%
!! TTTTGCATTTTAGTGGAACA+CGG + chr3_1:70424179-70424198 Msa0372110:three_prime_UTR 30.0%
TTCTCATTAAATTGTCGAGA+TGG - chr3_1:70424567-70424586 None:intergenic 30.0%
TTGGATATTGTAACAATGAC+AGG + chr3_1:70424998-70425017 Msa0372110:three_prime_UTR 30.0%
AAAAGGGAAAAGGGAAAAGT+GGG - chr3_1:70421696-70421715 None:intergenic 35.0%
GATGTTGTTGAAATCAGACT+TGG - chr3_1:70421823-70421842 None:intergenic 35.0%
! TGACTTGGGTTTGATATGAA+CGG - chr3_1:70421881-70421900 None:intergenic 35.0%
AAACAACAGAAGGGCAAAAA+AGG + chr3_1:70422379-70422398 Msa0372110:intron 35.0%
AGAGATCAGACTTCTTAGTT+GGG - chr3_1:70422449-70422468 None:intergenic 35.0%
CTAATCAGCAACTCAATTCA+AGG - chr3_1:70422571-70422590 None:intergenic 35.0%
AAAGAAGAAACATACCGCAA+GGG - chr3_1:70422715-70422734 None:intergenic 35.0%
AAGAGCAGAATCATACATTG+AGG - chr3_1:70422880-70422899 None:intergenic 35.0%
!! CTTTGGAATCTTTACACTTC+TGG + chr3_1:70423067-70423086 Msa0372110:CDS 35.0%
AGAAAACAAAAGCCAGACAA+CGG - chr3_1:70423138-70423157 None:intergenic 35.0%
TCCATGCAAATAAGATAGGT+AGG - chr3_1:70423165-70423184 None:intergenic 35.0%
ATGCTCCATGCAAATAAGAT+AGG - chr3_1:70423169-70423188 None:intergenic 35.0%
! TATCTTATTTGCATGGAGCA+TGG + chr3_1:70423168-70423187 Msa0372110:CDS 35.0%
! ACACGGATTTTGCATTTTAG+TGG + chr3_1:70424172-70424191 Msa0372110:three_prime_UTR 35.0%
!! TTTGCATTTTAGTGGAACAC+GGG + chr3_1:70424180-70424199 Msa0372110:three_prime_UTR 35.0%
TTCTCATTAAATTGCCGAGA+TGG - chr3_1:70424542-70424561 None:intergenic 35.0%
AATCTCAGACTAAGCTGAAT+TGG - chr3_1:70424699-70424718 None:intergenic 35.0%
!!! GCTTAGTCTGAGATTAGTTT+TGG + chr3_1:70424704-70424723 Msa0372110:three_prime_UTR 35.0%
AATAGACTAAGTTGTGAGAC+TGG + chr3_1:70424727-70424746 Msa0372110:three_prime_UTR 35.0%
!!! TAAGTTGTGAGACTGGTTTT+TGG + chr3_1:70424734-70424753 Msa0372110:three_prime_UTR 35.0%
! TTTTAGTCTAACTCTTAGCC+TGG + chr3_1:70424862-70424881 Msa0372110:three_prime_UTR 35.0%
! GCATAGGCTATGTTTTACTT+TGG + chr3_1:70424889-70424908 Msa0372110:three_prime_UTR 35.0%
GAAAAGGGAAAAGGGAAAAG+TGG - chr3_1:70421697-70421716 None:intergenic 40.0%
GGGAAAGTGAAAAGGGAAAA+GGG - chr3_1:70421705-70421724 None:intergenic 40.0%
AGGGAAAGTGAAAAGGGAAA+AGG - chr3_1:70421706-70421725 None:intergenic 40.0%
GGGAAAAGGGAAAGTGAAAA+GGG - chr3_1:70421712-70421731 None:intergenic 40.0%
AGGGAAAAGGGAAAGTGAAA+AGG - chr3_1:70421713-70421732 None:intergenic 40.0%
ATTGTTCGATTGAGAACGGT+TGG - chr3_1:70421767-70421786 None:intergenic 40.0%
!! AGACTTGGTGAAGGTTGAAT+CGG - chr3_1:70421808-70421827 None:intergenic 40.0%
!! GTTGAAATCAGACTTGGTGA+AGG - chr3_1:70421817-70421836 None:intergenic 40.0%
TGAATCGAACCAAACAGCAA+AGG + chr3_1:70421941-70421960 Msa0372110:five_prime_UTR 40.0%
!! ATTCGGTTACCTTTGCTGTT+TGG - chr3_1:70421953-70421972 None:intergenic 40.0%
TTCTTCTTCCTTCGCGAATT+CGG - chr3_1:70421970-70421989 None:intergenic 40.0%
CAACAACAAACACAAGCTGT+CGG + chr3_1:70422024-70422043 Msa0372110:five_prime_UTR 40.0%
!! AAAGAATAGAGCCGTTTTCG+TGG + chr3_1:70422073-70422092 Msa0372110:five_prime_UTR 40.0%
!! TCCAACGAGATTTGCTATTC+CGG - chr3_1:70422280-70422299 None:intergenic 40.0%
GCTGCTTACAAACAACAGAA+GGG + chr3_1:70422370-70422389 Msa0372110:intron 40.0%
GAGAGATCAGACTTCTTAGT+TGG - chr3_1:70422450-70422469 None:intergenic 40.0%
TCAACAACTGTACTCAAACC+TGG + chr3_1:70422479-70422498 Msa0372110:five_prime_UTR 40.0%
CTGTACTCAAACCTGGAAAA+CGG + chr3_1:70422486-70422505 Msa0372110:five_prime_UTR 40.0%
ACAGTACATTGCGATGAACA+AGG + chr3_1:70422596-70422615 Msa0372110:exon 40.0%
AAGAACTCTTTCTGCTTCCA+GGG - chr3_1:70422642-70422661 None:intergenic 40.0%
AAAGAACTCTTTCTGCTTCC+AGG - chr3_1:70422643-70422662 None:intergenic 40.0%
GAAAGAAGAAACATACCGCA+AGG - chr3_1:70422716-70422735 None:intergenic 40.0%
CCAAAGACAAGTGTAAGAAC+AGG - chr3_1:70423053-70423072 None:intergenic 40.0%
CCTGTTCTTACACTTGTCTT+TGG + chr3_1:70423050-70423069 Msa0372110:CDS 40.0%
ACTTCTGGCCAATTTGATGA+GGG + chr3_1:70423082-70423101 Msa0372110:CDS 40.0%
GAAGAAAGCCCTCATCAAAT+TGG - chr3_1:70423093-70423112 None:intergenic 40.0%
! GCCTACCTATCTTATTTGCA+TGG + chr3_1:70423161-70423180 Msa0372110:CDS 40.0%
TATTTGCATGGAGCATGGTA+TGG + chr3_1:70423173-70423192 Msa0372110:intron 40.0%
TTAAATATGGCACAGGAACC+AGG - chr3_1:70423928-70423947 None:intergenic 40.0%
TACTCCCATAGATTGTCTCA+AGG + chr3_1:70424436-70424455 Msa0372110:three_prime_UTR 40.0%
ACTCCCATAGATTGTCTCAA+GGG + chr3_1:70424437-70424456 Msa0372110:three_prime_UTR 40.0%
ATCCCCTTGAGACAATCTAT+GGG - chr3_1:70424443-70424462 None:intergenic 40.0%
TATCCCCTTGAGACAATCTA+TGG - chr3_1:70424444-70424463 None:intergenic 40.0%
AAGAAATTCCACCTCCATCT+CGG + chr3_1:70424525-70424544 Msa0372110:three_prime_UTR 40.0%
TCATTAAATTGCCGAGATGG+AGG - chr3_1:70424539-70424558 None:intergenic 40.0%
AATGAGAAGTGATAGGAGCT+AGG + chr3_1:70424579-70424598 Msa0372110:three_prime_UTR 40.0%
TCTGATGTATAAGCTCCTGA+TGG - chr3_1:70424610-70424629 None:intergenic 40.0%
GAAGGGGAGAAAAGGGAAAA+GGG - chr3_1:70421725-70421744 None:intergenic 45.0%
TGAAGGGGAGAAAAGGGAAA+AGG - chr3_1:70421726-70421745 None:intergenic 45.0%
TGGAGAGTTTCACGAGTGAA+GGG - chr3_1:70421742-70421761 None:intergenic 45.0%
TTGGAGAGTTTCACGAGTGA+AGG - chr3_1:70421743-70421762 None:intergenic 45.0%
! TCGATTGAGAACGGTTGGTT+TGG - chr3_1:70421762-70421781 None:intergenic 45.0%
! CAGCTTTGTGCTTCTGACTT+GGG - chr3_1:70421895-70421914 None:intergenic 45.0%
! TCAGCTTTGTGCTTCTGACT+TGG - chr3_1:70421896-70421915 None:intergenic 45.0%
! CAGAAGCACAAAGCTGAGTT+TGG + chr3_1:70421899-70421918 Msa0372110:five_prime_UTR 45.0%
AAAGGTAACCGAATTCGCGA+AGG + chr3_1:70421959-70421978 Msa0372110:five_prime_UTR 45.0%
CCTCTGTTCTTCCACGAAAA+CGG - chr3_1:70422087-70422106 None:intergenic 45.0%
AGCAGCGATGGTTTCATAAG+AGG - chr3_1:70422356-70422375 None:intergenic 45.0%
! CTGTTGTTTGTAAGCAGCGA+TGG - chr3_1:70422368-70422387 None:intergenic 45.0%
CGCTGCTTACAAACAACAGA+AGG + chr3_1:70422369-70422388 Msa0372110:intron 45.0%
CTTCTTAGTTGGGAGATCGA+AGG - chr3_1:70422439-70422458 None:intergenic 45.0%
TCTCTTGCGATTCCACGATT+GGG + chr3_1:70422524-70422543 Msa0372110:five_prime_UTR 45.0%
! ACTATAAGAAGAGTGCACCC+TGG + chr3_1:70422622-70422641 Msa0372110:CDS 45.0%
ACAACTATTCGGTTTACGCC+CGG + chr3_1:70422835-70422854 Msa0372110:intron 45.0%
TGATTAGGTATGAGCCATCC+CGG - chr3_1:70422857-70422876 None:intergenic 45.0%
CACTTCTGGCCAATTTGATG+AGG + chr3_1:70423081-70423100 Msa0372110:CDS 45.0%
GCATCTGAGGGGAAACTAAA+TGG + chr3_1:70423834-70423853 Msa0372110:exon 45.0%
CTGAGGGGAAACTAAATGGT+TGG + chr3_1:70423838-70423857 Msa0372110:exon 45.0%
GGCGAAAAGAACGAAGACAT+AGG + chr3_1:70424393-70424412 Msa0372110:three_prime_UTR 45.0%
CTCCCATAGATTGTCTCAAG+GGG + chr3_1:70424438-70424457 Msa0372110:three_prime_UTR 45.0%
TTAAATTGCCGAGATGGAGG+TGG - chr3_1:70424536-70424555 None:intergenic 45.0%
!!! GTGAGACTGGTTTTTGGAGT+AGG + chr3_1:70424740-70424759 Msa0372110:three_prime_UTR 45.0%
!!! AGACTGGTTTTTGGAGTAGG+TGG + chr3_1:70424743-70424762 Msa0372110:three_prime_UTR 45.0%
!!! GGTTTTTGGAGTAGGTGGTT+CGG + chr3_1:70424748-70424767 Msa0372110:three_prime_UTR 45.0%
GGGAAAAGGGAAAAGTGGGA+GGG - chr3_1:70421692-70421711 None:intergenic 50.0%
AGGGAAAAGGGAAAAGTGGG+AGG - chr3_1:70421693-70421712 None:intergenic 50.0%
CACGAGTGAAGGGGAGAAAA+GGG - chr3_1:70421732-70421751 None:intergenic 50.0%
TCACGAGTGAAGGGGAGAAA+AGG - chr3_1:70421733-70421752 None:intergenic 50.0%
GGAGAGTTTCACGAGTGAAG+GGG - chr3_1:70421741-70421760 None:intergenic 50.0%
! CCGTTTTCGTGGAAGAACAG+AGG + chr3_1:70422084-70422103 Msa0372110:five_prime_UTR 50.0%
! AAGAGTTTGAAACTCCGGCG+CGG + chr3_1:70422231-70422250 Msa0372110:intron 50.0%
GCCGGAATAGCAAATCTCGT+TGG + chr3_1:70422276-70422295 Msa0372110:intron 50.0%
TTGGGAGATCGAAGGAAGAC+AGG - chr3_1:70422431-70422450 None:intergenic 50.0%
TCAAACCTGGAAAACGGACG+CGG + chr3_1:70422492-70422511 Msa0372110:five_prime_UTR 50.0%
TCGTGGAATCGCAAGAGAGA+AGG - chr3_1:70422522-70422541 None:intergenic 50.0%
CTCTCTTGCGATTCCACGAT+TGG + chr3_1:70422523-70422542 Msa0372110:five_prime_UTR 50.0%
CTCTTGCGATTCCACGATTG+GGG + chr3_1:70422525-70422544 Msa0372110:five_prime_UTR 50.0%
CAACTATTCGGTTTACGCCC+GGG + chr3_1:70422836-70422855 Msa0372110:intron 50.0%
GATTAGGTATGAGCCATCCC+GGG - chr3_1:70422856-70422875 None:intergenic 50.0%
AGGATGTCTCAGATGCTCAG+TGG + chr3_1:70423006-70423025 Msa0372110:intron 50.0%
ATGTCTCAGATGCTCAGTGG+AGG + chr3_1:70423009-70423028 Msa0372110:CDS 50.0%
! GCTCAGTGGAGGAATTTTCG+AGG + chr3_1:70423020-70423039 Msa0372110:CDS 50.0%
TGAGAGGAATGTCCGTTGTC+TGG + chr3_1:70423123-70423142 Msa0372110:CDS 50.0%
ACAGTATCCGACAGCATCTG+AGG + chr3_1:70423821-70423840 Msa0372110:CDS 50.0%
CAGTATCCGACAGCATCTGA+GGG + chr3_1:70423822-70423841 Msa0372110:CDS 50.0%
AGTATCCGACAGCATCTGAG+GGG + chr3_1:70423823-70423842 Msa0372110:CDS 50.0%
AGTTTCCCCTCAGATGCTGT+CGG - chr3_1:70423831-70423850 None:intergenic 50.0%
AGGAGCTAGGCTAAACCATC+AGG + chr3_1:70424592-70424611 Msa0372110:three_prime_UTR 50.0%
AACATAGCCTATGCACTGCC+AGG - chr3_1:70424883-70424902 None:intergenic 50.0%
GCAGCTCGAATCCGAAGAAG+AGG + chr3_1:70422049-70422068 Msa0372110:five_prime_UTR 55.0%
!! GATTTGCTATTCCGGCGAGG+TGG - chr3_1:70422272-70422291 None:intergenic 55.0%
!! CGAGATTTGCTATTCCGGCG+AGG - chr3_1:70422275-70422294 None:intergenic 55.0%
TGTGAATAGCGCCCCAATCG+TGG - chr3_1:70422539-70422558 None:intergenic 55.0%
TATTCGGTTTACGCCCGGGA+TGG + chr3_1:70422840-70422859 Msa0372110:intron 55.0%
CTCTTAGCCTGGCAGTGCAT+AGG + chr3_1:70424873-70424892 Msa0372110:three_prime_UTR 55.0%
! AGTTTGAAACTCCGGCGCGG+TGG + chr3_1:70422234-70422253 Msa0372110:intron 60.0%
! GAACACCGCGTCCGTTTTCC+AGG - chr3_1:70422500-70422519 None:intergenic 60.0%
CATCATCAGGCGCTCCCTTG+CGG + chr3_1:70422698-70422717 Msa0372110:CDS 60.0%
TGCTATTCCGGCGAGGTGGC+CGG - chr3_1:70422268-70422287 None:intergenic 65.0%
! CGGTGATGGCACCACCGCGC+CGG - chr3_1:70422248-70422267 None:intergenic 75.0%
!! CGGCGCGGTGGTGCCATCAC+CGG + chr3_1:70422246-70422265 Msa0372110:intron 75.0%
GCCATCACCGGCCACCTCGC+CGG + chr3_1:70422258-70422277 Msa0372110:intron 75.0%
TCCGGCGAGGTGGCCGGTGA+TGG - chr3_1:70422262-70422281 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 70421668 70425038 70421668 ID=Msa0372110;Name=Msa0372110
chr3_1 mRNA 70421668 70425038 70421668 ID=Msa0372110-mRNA-1;Parent=Msa0372110;Name=Msa0372110-mRNA-1;_AED=0.14;_eAED=0.14;_QI=659|1|1|1|0.6|0.5|6|966|127
chr3_1 exon 70421668 70422105 70421668 ID=Msa0372110-mRNA-1:exon:15303;Parent=Msa0372110-mRNA-1
chr3_1 exon 70422388 70422719 70422388 ID=Msa0372110-mRNA-1:exon:15304;Parent=Msa0372110-mRNA-1
chr3_1 exon 70423008 70423189 70423008 ID=Msa0372110-mRNA-1:exon:15305;Parent=Msa0372110-mRNA-1
chr3_1 exon 70423278 70423326 70423278 ID=Msa0372110-mRNA-1:exon:15306;Parent=Msa0372110-mRNA-1
chr3_1 exon 70423811 70423875 70423811 ID=Msa0372110-mRNA-1:exon:15307;Parent=Msa0372110-mRNA-1
chr3_1 exon 70424096 70425038 70424096 ID=Msa0372110-mRNA-1:exon:15308;Parent=Msa0372110-mRNA-1
chr3_1 five_prime_UTR 70421668 70422105 70421668 ID=Msa0372110-mRNA-1:five_prime_utr;Parent=Msa0372110-mRNA-1
chr3_1 five_prime_UTR 70422388 70422608 70422388 ID=Msa0372110-mRNA-1:five_prime_utr;Parent=Msa0372110-mRNA-1
chr3_1 CDS 70422609 70422719 70422609 ID=Msa0372110-mRNA-1:cds;Parent=Msa0372110-mRNA-1
chr3_1 CDS 70423008 70423189 70423008 ID=Msa0372110-mRNA-1:cds;Parent=Msa0372110-mRNA-1
chr3_1 CDS 70423278 70423326 70423278 ID=Msa0372110-mRNA-1:cds;Parent=Msa0372110-mRNA-1
chr3_1 CDS 70423811 70423852 70423811 ID=Msa0372110-mRNA-1:cds;Parent=Msa0372110-mRNA-1
chr3_1 three_prime_UTR 70423853 70423875 70423853 ID=Msa0372110-mRNA-1:three_prime_utr;Parent=Msa0372110-mRNA-1
chr3_1 three_prime_UTR 70424096 70425038 70424096 ID=Msa0372110-mRNA-1:three_prime_utr;Parent=Msa0372110-mRNA-1
Gene Sequence

>Msa0372110

ATGAACAAGGACAACTATAAGAAGAGTGCACCCTGGAAGCAGAAAGAGTTCTTTTTTCTCGTCACTTATGCATTCATTTTCTATGTTATCATCATCAGGCGCTCCCTTGCGGATGTCTCAGATGCTCAGTGGAGGAATTTTCGAGGAAATTTACCTGTTCTTACACTTGTCTTTGGAATCTTTACACTTCTGGCCAATTTGATGAGGGCTTTCTTCAATTTCAATGTGAGAGGAATGTCCGTTGTCTGGCTTTTGTTTTCTTTCGCCTACCTATCTTATTTGCATGGAGCATGCGTCATATTTGTGCTCTCAATAGCTACTATTAATTTTCTTCTTGTTAAGTCATTGTCGAACAGTATCCGACAGCATCTGAGGGGAAACTAA
Protein sequence

>Msa0372110

MNKDNYKKSAPWKQKEFFFLVTYAFIFYVIIIRRSLADVSDAQWRNFRGNLPVLTLVFGIFTLLANLMRAFFNFNVRGMSVVWLLFSFAYLSYLHGACVIFVLSIATINFLLVKSLSNSIRQHLRGN*