Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0382610 | AES73668.1 | 96.078 | 153 | 3 | 2 | 1 | 153 | 1 | 150 | 2.26e-102 | 300 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0382610 | sp|Q9ZV52|EGC2_ARATH | 37.879 | 132 | 68 | 5 | 23 | 151 | 7 | 127 | 2.01e-18 | 79.0 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0382610 | G7JB40 | 96.078 | 153 | 3 | 2 | 1 | 153 | 1 | 150 | 1.08e-102 | 300 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0059880 | Msa0382610 | 0.807747 | 4.346288e-50 | -8.615850e-47 |
| Msa0143710 | Msa0382610 | 0.801611 | 8.299809e-49 | -8.615850e-47 |
| Msa0230140 | Msa0382610 | 0.972814 | 2.197172e-135 | -8.615850e-47 |
| Msa0382610 | Msa0480400 | 0.941984 | 1.600372e-101 | -8.615850e-47 |
| Msa0382610 | Msa0524930 | 0.873226 | 1.732870e-67 | -8.615850e-47 |
| Msa0382610 | Msa0646210 | 0.804699 | 1.905984e-49 | -8.615850e-47 |
| Msa0382610 | Msa1109890 | 0.801407 | 9.135676e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0382610 | MtrunA17_Chr3g0137931 | 96.078 | 153 | 3 | 2 | 1 | 153 | 1 | 150 | 2.08e-106 | 300 |
| Msa0382610 | MtrunA17_Chr6g0477991 | 93.750 | 96 | 6 | 0 | 58 | 153 | 7 | 102 | 5.70e-65 | 193 |
| Msa0382610 | MtrunA17_Chr6g0476161 | 87.879 | 66 | 8 | 0 | 76 | 141 | 10 | 75 | 1.15e-38 | 127 |
| Msa0382610 | MtrunA17_Chr3g0138201 | 61.538 | 52 | 20 | 0 | 43 | 94 | 28 | 79 | 4.17e-19 | 76.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0382610 | AT2G18660.1 | 37.879 | 132 | 68 | 5 | 23 | 151 | 7 | 127 | 2.05e-19 | 79.0 |
| Msa0382610 | AT4G30380.1 | 33.333 | 132 | 68 | 6 | 27 | 152 | 5 | 122 | 5.29e-11 | 57.0 |
Find 31 sgRNAs with CRISPR-Local
Find 112 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTGGTACTGCTTCTTCTTA+TGG | 0.173763 | 3_1:-85270515 | Msa0382610:CDS |
| CAACATTGATATTTGCATTA+GGG | 0.294856 | 3_1:+85269712 | None:intergenic |
| TGCTCATTTGTTTGAGCAAT+AGG | 0.304172 | 3_1:+85270552 | None:intergenic |
| TCAACATTGATATTTGCATT+AGG | 0.306005 | 3_1:+85269711 | None:intergenic |
| ACTGCCACAAATATATTTCC+TGG | 0.404012 | 3_1:+85269930 | None:intergenic |
| CTACAGCTTGTGATGGGAAT+AGG | 0.406360 | 3_1:-85269968 | Msa0382610:CDS |
| TGGGTGTACTCACGTATATA+TGG | 0.432788 | 3_1:+85270488 | None:intergenic |
| AATGAAGGGTTGTGGGATAA+TGG | 0.441192 | 3_1:-85269906 | Msa0382610:CDS |
| AGCATTATTCTTGCAGATGT+TGG | 0.481138 | 3_1:-85270533 | Msa0382610:CDS |
| TGGCAGTGAATGAAGGGTTG+TGG | 0.485625 | 3_1:-85269914 | Msa0382610:CDS |
| ATATTTGTGGCAGTGAATGA+AGG | 0.490865 | 3_1:-85269921 | Msa0382610:CDS |
| TACAGAGTAAGGTGTGTAAG+TGG | 0.500626 | 3_1:-85269864 | Msa0382610:CDS |
| GGCAGTGAATGAAGGGTTGT+GGG | 0.502397 | 3_1:-85269913 | Msa0382610:CDS |
| ATCAATAAGCCATGCAAAGG+TGG | 0.523686 | 3_1:-85269840 | Msa0382610:CDS |
| TCCACCAGGAAATATATTTG+TGG | 0.549161 | 3_1:-85269934 | Msa0382610:CDS |
| AGTTGACATGTGGAAAGTGT+GGG | 0.573051 | 3_1:+85269761 | None:intergenic |
| CAGTTGACATGTGGAAAGTG+TGG | 0.576339 | 3_1:+85269760 | None:intergenic |
| ATACTACTACCACCTTTGCA+TGG | 0.578652 | 3_1:+85269831 | None:intergenic |
| GTGGAAGGAGATACAGAGTA+AGG | 0.582766 | 3_1:-85269875 | Msa0382610:CDS |
| TGGGATAATGGTGCAGCATG+TGG | 0.603337 | 3_1:-85269894 | Msa0382610:CDS |
| GGAATCAATAAGCCATGCAA+AGG | 0.623535 | 3_1:-85269843 | Msa0382610:CDS |
| TATTTGTGGCAGTGAATGAA+GGG | 0.626127 | 3_1:-85269920 | Msa0382610:CDS |
| TGTGCAGCTACAGCTTGTGA+TGG | 0.627923 | 3_1:-85269975 | Msa0382610:intron |
| GTGCAGCTACAGCTTGTGAT+GGG | 0.628864 | 3_1:-85269974 | Msa0382610:intron |
| GCCACAAATATATTTCCTGG+TGG | 0.638508 | 3_1:+85269933 | None:intergenic |
| CAAATGCTTCAGTTGACATG+TGG | 0.644023 | 3_1:+85269751 | None:intergenic |
| ATAATGGTGCAGCATGTGGA+AGG | 0.648736 | 3_1:-85269890 | Msa0382610:CDS |
| AGGAGACAACAGTTTCCACC+AGG | 0.667613 | 3_1:-85269948 | Msa0382610:CDS |
| GTGTACTCACGTATATATGG+AGG | 0.706847 | 3_1:+85270491 | None:intergenic |
| TTGACATGTGGAAAGTGTGG+GGG | 0.720155 | 3_1:+85269763 | None:intergenic |
| GTTGACATGTGGAAAGTGTG+GGG | 0.728941 | 3_1:+85269762 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGAAATATTGAATGAATAAA+TGG | + | chr3_1:85269883-85269902 | None:intergenic | 15.0% |
| !! | AGAAATATTGAATGAATAAA+TGG | + | chr3_1:85269883-85269902 | None:intergenic | 15.0% |
| !!! | AACTATAATTTTTTGCTTGA+TGG | + | chr3_1:85269706-85269725 | None:intergenic | 20.0% |
| !! | ATATTGAATGAATAAATGGT+GGG | + | chr3_1:85269879-85269898 | None:intergenic | 20.0% |
| !! | AATATTGAATGAATAAATGG+TGG | + | chr3_1:85269880-85269899 | None:intergenic | 20.0% |
| !! | ATTTAACGTGGAAAAAAAAT+TGG | + | chr3_1:85269941-85269960 | None:intergenic | 20.0% |
| !!! | GTTCCAAACATTTTAAAAAT+GGG | + | chr3_1:85270193-85270212 | None:intergenic | 20.0% |
| !! | ATTAATATGTGAAATTTGTG+TGG | + | chr3_1:85270215-85270234 | None:intergenic | 20.0% |
| !!! | AACTATAATTTTTTGCTTGA+TGG | + | chr3_1:85269706-85269725 | None:intergenic | 20.0% |
| !! | ATATTGAATGAATAAATGGT+GGG | + | chr3_1:85269879-85269898 | None:intergenic | 20.0% |
| !! | AATATTGAATGAATAAATGG+TGG | + | chr3_1:85269880-85269899 | None:intergenic | 20.0% |
| !! | ATTTAACGTGGAAAAAAAAT+TGG | + | chr3_1:85269941-85269960 | None:intergenic | 20.0% |
| !!! | GTTCCAAACATTTTAAAAAT+GGG | + | chr3_1:85270193-85270212 | None:intergenic | 20.0% |
| !! | ATTAATATGTGAAATTTGTG+TGG | + | chr3_1:85270215-85270234 | None:intergenic | 20.0% |
| ! | TAGCTCAAAAACAAATAATG+AGG | - | chr3_1:85269741-85269760 | Msa0382610:CDS | 25.0% |
| ! | ATGATATATCATGTATACGT+AGG | + | chr3_1:85269910-85269929 | None:intergenic | 25.0% |
| ! | AGTGTTAAACTAAATGTTGA+TGG | + | chr3_1:85270125-85270144 | None:intergenic | 25.0% |
| !!! | AGTCCCATTTTTAAAATGTT+TGG | - | chr3_1:85270187-85270206 | Msa0382610:intron | 25.0% |
| !!! | GGTTCCAAACATTTTAAAAA+TGG | + | chr3_1:85270194-85270213 | None:intergenic | 25.0% |
| ! | CAACATTGATATTTGCATTA+GGG | + | chr3_1:85270636-85270655 | None:intergenic | 25.0% |
| ! | TCAACATTGATATTTGCATT+AGG | + | chr3_1:85270637-85270656 | None:intergenic | 25.0% |
| ! | TAGCTCAAAAACAAATAATG+AGG | - | chr3_1:85269741-85269760 | Msa0382610:CDS | 25.0% |
| ! | ATGATATATCATGTATACGT+AGG | + | chr3_1:85269910-85269929 | None:intergenic | 25.0% |
| ! | AGTGTTAAACTAAATGTTGA+TGG | + | chr3_1:85270125-85270144 | None:intergenic | 25.0% |
| !!! | AGTCCCATTTTTAAAATGTT+TGG | - | chr3_1:85270187-85270206 | Msa0382610:intron | 25.0% |
| !!! | GGTTCCAAACATTTTAAAAA+TGG | + | chr3_1:85270194-85270213 | None:intergenic | 25.0% |
| ! | CAACATTGATATTTGCATTA+GGG | + | chr3_1:85270636-85270655 | None:intergenic | 25.0% |
| ! | TCAACATTGATATTTGCATT+AGG | + | chr3_1:85270637-85270656 | None:intergenic | 25.0% |
| !! | AAAGAGATTAGTCTCCTATA+AGG | + | chr3_1:85269995-85270014 | None:intergenic | 30.0% |
| AACCAATCGGTATCATTATA+GGG | + | chr3_1:85270024-85270043 | None:intergenic | 30.0% | |
| !! | AAAGAGATTAGTCTCCTATA+AGG | + | chr3_1:85269995-85270014 | None:intergenic | 30.0% |
| AACCAATCGGTATCATTATA+GGG | + | chr3_1:85270024-85270043 | None:intergenic | 30.0% | |
| TGCTCATTTGTTTGAGCAAT+AGG | + | chr3_1:85269796-85269815 | None:intergenic | 35.0% | |
| AGCATTATTCTTGCAGATGT+TGG | - | chr3_1:85269812-85269831 | Msa0382610:CDS | 35.0% | |
| !! | AGAGGTGTTGATATTTAACG+TGG | + | chr3_1:85269953-85269972 | None:intergenic | 35.0% |
| AGTTTAGTGCATCACCTTAT+AGG | - | chr3_1:85269978-85269997 | Msa0382610:intron | 35.0% | |
| ACCAATCGGTATCATTATAG+GGG | + | chr3_1:85270023-85270042 | None:intergenic | 35.0% | |
| CAACCAATCGGTATCATTAT+AGG | + | chr3_1:85270025-85270044 | None:intergenic | 35.0% | |
| ! | CTATAATGATACCGATTGGT+TGG | - | chr3_1:85270023-85270042 | Msa0382610:intron | 35.0% |
| TCCACCAGGAAATATATTTG+TGG | - | chr3_1:85270411-85270430 | Msa0382610:intron | 35.0% | |
| ACTGCCACAAATATATTTCC+TGG | + | chr3_1:85270418-85270437 | None:intergenic | 35.0% | |
| ATATTTGTGGCAGTGAATGA+AGG | - | chr3_1:85270424-85270443 | Msa0382610:intron | 35.0% | |
| TATTTGTGGCAGTGAATGAA+GGG | - | chr3_1:85270425-85270444 | Msa0382610:intron | 35.0% | |
| TGCTCATTTGTTTGAGCAAT+AGG | + | chr3_1:85269796-85269815 | None:intergenic | 35.0% | |
| AGCATTATTCTTGCAGATGT+TGG | - | chr3_1:85269812-85269831 | Msa0382610:CDS | 35.0% | |
| !! | AGAGGTGTTGATATTTAACG+TGG | + | chr3_1:85269953-85269972 | None:intergenic | 35.0% |
| AGTTTAGTGCATCACCTTAT+AGG | - | chr3_1:85269978-85269997 | Msa0382610:intron | 35.0% | |
| ACCAATCGGTATCATTATAG+GGG | + | chr3_1:85270023-85270042 | None:intergenic | 35.0% | |
| CAACCAATCGGTATCATTAT+AGG | + | chr3_1:85270025-85270044 | None:intergenic | 35.0% | |
| ! | CTATAATGATACCGATTGGT+TGG | - | chr3_1:85270023-85270042 | Msa0382610:intron | 35.0% |
| TCCACCAGGAAATATATTTG+TGG | - | chr3_1:85270411-85270430 | Msa0382610:intron | 35.0% | |
| ACTGCCACAAATATATTTCC+TGG | + | chr3_1:85270418-85270437 | None:intergenic | 35.0% | |
| ATATTTGTGGCAGTGAATGA+AGG | - | chr3_1:85270424-85270443 | Msa0382610:intron | 35.0% | |
| TATTTGTGGCAGTGAATGAA+GGG | - | chr3_1:85270425-85270444 | Msa0382610:intron | 35.0% | |
| GTTGGTACTGCTTCTTCTTA+TGG | - | chr3_1:85269830-85269849 | Msa0382610:CDS | 40.0% | |
| GTGTACTCACGTATATATGG+AGG | + | chr3_1:85269857-85269876 | None:intergenic | 40.0% | |
| TGGGTGTACTCACGTATATA+TGG | + | chr3_1:85269860-85269879 | None:intergenic | 40.0% | |
| GATGCACTAAACTACAAGAG+AGG | + | chr3_1:85269971-85269990 | None:intergenic | 40.0% | |
| ACCCCTATAATGATACCGAT+TGG | - | chr3_1:85270019-85270038 | Msa0382610:intron | 40.0% | |
| ! | TAATGATACCGATTGGTTGG+AGG | - | chr3_1:85270026-85270045 | Msa0382610:intron | 40.0% |
| ! | TTTTACCACCTCCAACCAAT+CGG | + | chr3_1:85270037-85270056 | None:intergenic | 40.0% |
| GCCACAAATATATTTCCTGG+TGG | + | chr3_1:85270415-85270434 | None:intergenic | 40.0% | |
| AATGAAGGGTTGTGGGATAA+TGG | - | chr3_1:85270439-85270458 | Msa0382610:intron | 40.0% | |
| TACAGAGTAAGGTGTGTAAG+TGG | - | chr3_1:85270481-85270500 | Msa0382610:intron | 40.0% | |
| GGAATCAATAAGCCATGCAA+AGG | - | chr3_1:85270502-85270521 | Msa0382610:CDS | 40.0% | |
| ATCAATAAGCCATGCAAAGG+TGG | - | chr3_1:85270505-85270524 | Msa0382610:CDS | 40.0% | |
| ATACTACTACCACCTTTGCA+TGG | + | chr3_1:85270517-85270536 | None:intergenic | 40.0% | |
| ! | AGTTGACATGTGGAAAGTGT+GGG | + | chr3_1:85270587-85270606 | None:intergenic | 40.0% |
| CAAATGCTTCAGTTGACATG+TGG | + | chr3_1:85270597-85270616 | None:intergenic | 40.0% | |
| GTTGGTACTGCTTCTTCTTA+TGG | - | chr3_1:85269830-85269849 | Msa0382610:CDS | 40.0% | |
| GTGTACTCACGTATATATGG+AGG | + | chr3_1:85269857-85269876 | None:intergenic | 40.0% | |
| TGGGTGTACTCACGTATATA+TGG | + | chr3_1:85269860-85269879 | None:intergenic | 40.0% | |
| GATGCACTAAACTACAAGAG+AGG | + | chr3_1:85269971-85269990 | None:intergenic | 40.0% | |
| ACCCCTATAATGATACCGAT+TGG | - | chr3_1:85270019-85270038 | Msa0382610:intron | 40.0% | |
| ! | TAATGATACCGATTGGTTGG+AGG | - | chr3_1:85270026-85270045 | Msa0382610:intron | 40.0% |
| ! | TTTTACCACCTCCAACCAAT+CGG | + | chr3_1:85270037-85270056 | None:intergenic | 40.0% |
| GCCACAAATATATTTCCTGG+TGG | + | chr3_1:85270415-85270434 | None:intergenic | 40.0% | |
| AATGAAGGGTTGTGGGATAA+TGG | - | chr3_1:85270439-85270458 | Msa0382610:intron | 40.0% | |
| TACAGAGTAAGGTGTGTAAG+TGG | - | chr3_1:85270481-85270500 | Msa0382610:intron | 40.0% | |
| GGAATCAATAAGCCATGCAA+AGG | - | chr3_1:85270502-85270521 | Msa0382610:CDS | 40.0% | |
| ATCAATAAGCCATGCAAAGG+TGG | - | chr3_1:85270505-85270524 | Msa0382610:CDS | 40.0% | |
| ATACTACTACCACCTTTGCA+TGG | + | chr3_1:85270517-85270536 | None:intergenic | 40.0% | |
| ! | AGTTGACATGTGGAAAGTGT+GGG | + | chr3_1:85270587-85270606 | None:intergenic | 40.0% |
| CAAATGCTTCAGTTGACATG+TGG | + | chr3_1:85270597-85270616 | None:intergenic | 40.0% | |
| ! | CTACAGCTTGTGATGGGAAT+AGG | - | chr3_1:85270377-85270396 | Msa0382610:intron | 45.0% |
| ATAATGGTGCAGCATGTGGA+AGG | - | chr3_1:85270455-85270474 | Msa0382610:intron | 45.0% | |
| GTGGAAGGAGATACAGAGTA+AGG | - | chr3_1:85270470-85270489 | Msa0382610:intron | 45.0% | |
| ! | TTGACATGTGGAAAGTGTGG+GGG | + | chr3_1:85270585-85270604 | None:intergenic | 45.0% |
| ! | GTTGACATGTGGAAAGTGTG+GGG | + | chr3_1:85270586-85270605 | None:intergenic | 45.0% |
| CAGTTGACATGTGGAAAGTG+TGG | + | chr3_1:85270588-85270607 | None:intergenic | 45.0% | |
| ! | CTACAGCTTGTGATGGGAAT+AGG | - | chr3_1:85270377-85270396 | Msa0382610:intron | 45.0% |
| ATAATGGTGCAGCATGTGGA+AGG | - | chr3_1:85270455-85270474 | Msa0382610:intron | 45.0% | |
| GTGGAAGGAGATACAGAGTA+AGG | - | chr3_1:85270470-85270489 | Msa0382610:intron | 45.0% | |
| ! | TTGACATGTGGAAAGTGTGG+GGG | + | chr3_1:85270585-85270604 | None:intergenic | 45.0% |
| ! | GTTGACATGTGGAAAGTGTG+GGG | + | chr3_1:85270586-85270605 | None:intergenic | 45.0% |
| CAGTTGACATGTGGAAAGTG+TGG | + | chr3_1:85270588-85270607 | None:intergenic | 45.0% | |
| ! | TGATACCGATTGGTTGGAGG+TGG | - | chr3_1:85270029-85270048 | Msa0382610:intron | 50.0% |
| TGTGCAGCTACAGCTTGTGA+TGG | - | chr3_1:85270370-85270389 | Msa0382610:intron | 50.0% | |
| GTGCAGCTACAGCTTGTGAT+GGG | - | chr3_1:85270371-85270390 | Msa0382610:intron | 50.0% | |
| AGGAGACAACAGTTTCCACC+AGG | - | chr3_1:85270397-85270416 | Msa0382610:intron | 50.0% | |
| TGGCAGTGAATGAAGGGTTG+TGG | - | chr3_1:85270431-85270450 | Msa0382610:intron | 50.0% | |
| GGCAGTGAATGAAGGGTTGT+GGG | - | chr3_1:85270432-85270451 | Msa0382610:intron | 50.0% | |
| ! | TGGGATAATGGTGCAGCATG+TGG | - | chr3_1:85270451-85270470 | Msa0382610:intron | 50.0% |
| ! | TGATACCGATTGGTTGGAGG+TGG | - | chr3_1:85270029-85270048 | Msa0382610:intron | 50.0% |
| TGTGCAGCTACAGCTTGTGA+TGG | - | chr3_1:85270370-85270389 | Msa0382610:intron | 50.0% | |
| GTGCAGCTACAGCTTGTGAT+GGG | - | chr3_1:85270371-85270390 | Msa0382610:intron | 50.0% | |
| AGGAGACAACAGTTTCCACC+AGG | - | chr3_1:85270397-85270416 | Msa0382610:intron | 50.0% | |
| TGGCAGTGAATGAAGGGTTG+TGG | - | chr3_1:85270431-85270450 | Msa0382610:intron | 50.0% | |
| GGCAGTGAATGAAGGGTTGT+GGG | - | chr3_1:85270432-85270451 | Msa0382610:intron | 50.0% | |
| ! | TGGGATAATGGTGCAGCATG+TGG | - | chr3_1:85270451-85270470 | Msa0382610:intron | 50.0% |
| ! | GTGGAAAGTGTGGGGGCATG+AGG | + | chr3_1:85270578-85270597 | None:intergenic | 60.0% |
| ! | GTGGAAAGTGTGGGGGCATG+AGG | + | chr3_1:85270578-85270597 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_1 | gene | 85269698 | 85270669 | 85269698 | ID=Msa0382610;Name=Msa0382610 |
| chr3_1 | mRNA | 85269698 | 85270669 | 85269698 | ID=Msa0382610-mRNA-1;Parent=Msa0382610;Name=Msa0382610-mRNA-1;_AED=0.00;_eAED=0.01;_QI=0|0|0|1|1|1|2|0|153 |
| chr3_1 | exon | 85270501 | 85270669 | 85270501 | ID=Msa0382610-mRNA-1:exon:22324;Parent=Msa0382610-mRNA-1 |
| chr3_1 | exon | 85269698 | 85269990 | 85269698 | ID=Msa0382610-mRNA-1:exon:22323;Parent=Msa0382610-mRNA-1 |
| chr3_1 | CDS | 85270501 | 85270669 | 85270501 | ID=Msa0382610-mRNA-1:cds;Parent=Msa0382610-mRNA-1 |
| chr3_1 | CDS | 85269698 | 85269990 | 85269698 | ID=Msa0382610-mRNA-1:cds;Parent=Msa0382610-mRNA-1 |
| Gene Sequence |
| Protein sequence |
