AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0383240

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0383240 MtrunA17_Chr3g0138631 99.381 323 2 0 1 323 1 323 0.0 642
Msa0383240 MtrunA17_Chr5g0436791 91.331 323 28 0 1 323 1 323 0.0 556
Msa0383240 MtrunA17_Chr1g0168061 98.765 81 1 0 243 323 1 81 8.00e-48 155
Msa0383240 MtrunA17_Chr7g0222411 68.269 104 32 1 11 113 7 110 8.94e-42 143
Msa0383240 MtrunA17_Chr7g0272291 68.539 89 27 1 11 98 7 95 2.80e-34 120
Msa0383240 MtrunA17_Chr3g0115971 54.630 108 27 2 63 169 8 94 1.79e-22 92.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0383240 AT2G40010.1 83.801 321 48 1 1 321 1 317 0.0 520
Msa0383240 AT3G09200.1 89.493 276 29 0 3 278 2 277 6.37e-180 500
Msa0383240 AT3G11250.1 88.406 276 32 0 3 278 2 277 4.98e-178 495
Msa0383240 AT3G09200.2 78.623 276 26 1 3 278 2 244 2.36e-149 421
Msa0383240 AT1G25260.1 22.685 216 146 4 10 214 16 221 4.06e-11 62.4

Find 136 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAACTAAGATAGGTATTT+TGG 0.121345 3_1:+86017081 Msa0383240:five_prime_UTR
TCCTCCTTGGCAGCTGCAGC+AGG 0.147360 3_1:-86019597 None:intergenic
CAAACCCAGTGGGCCTAAAA+AGG 0.215339 3_1:+86017193 Msa0383240:five_prime_UTR
ATCTCAGACCTCTTTCTTCC+AGG 0.252116 3_1:+86018965 Msa0383240:CDS
CAGCGTTGACAACAAGGATT+TGG 0.257235 3_1:-86017508 None:intergenic
GCCCCAGCTGCTGCTGCGTC+TGG 0.268454 3_1:+86019567 Msa0383240:CDS
CATGGTCACTTCACTTTCAT+TGG 0.278087 3_1:+86019297 Msa0383240:CDS
CACCAGACGCAGCAGCAGCT+GGG 0.284716 3_1:-86019569 None:intergenic
GTGACTTGTGTTTGCCTCCT+AGG 0.293721 3_1:+86019849 Msa0383240:three_prime_UTR
CCACCAGACGCAGCAGCAGC+TGG 0.308640 3_1:-86019570 None:intergenic
ATCGTAGTTCTGCTTCTTCT+CGG 0.310851 3_1:-86017453 None:intergenic
CACAGTACCCTTGTTAATCT+TGG 0.311903 3_1:-86019071 None:intergenic
GATTTAAGAGAGGAAATTAA+AGG 0.326559 3_1:-86017375 None:intergenic
TTGTAGCGAAGTTTGCTATT+GGG 0.333941 3_1:+86019269 Msa0383240:CDS
TCAAAACTTCAGAATGGTTT+GGG 0.337661 3_1:-86017062 None:intergenic
TTCAAAACTTCAGAATGGTT+TGG 0.345461 3_1:-86017063 None:intergenic
TCTTCATCAGACTCTTCTTC+AGG 0.361468 3_1:-86019636 None:intergenic
GCGTTCGGTAGTCAAATTAC+TGG 0.376848 3_1:+86019888 Msa0383240:three_prime_UTR
GCTGCATTGCTTGCCAAGTT+AGG 0.382522 3_1:+86019151 Msa0383240:CDS
TCCTTAACCTTGTCTGCCTC+AGG 0.389206 3_1:-86019415 None:intergenic
CTTGTAGCGAAGTTTGCTAT+TGG 0.396386 3_1:+86019268 Msa0383240:CDS
GTTGAGAAAAGCATTGTTGC+CGG 0.397292 3_1:-86017648 None:intergenic
GTTCTGCTTCTTCTCGGCTT+TGG 0.398033 3_1:-86017447 None:intergenic
AGCTCCTGCTCGTGTTGGTT+TGG 0.398892 3_1:+86018893 Msa0383240:CDS
CTCCTTGGCAGCTGCAGCAG+GGG 0.398915 3_1:-86019595 None:intergenic
TTCCTTCTTTGCCTCCTCCT+TGG 0.403009 3_1:-86019610 None:intergenic
AAAAGCAAGAACGTTCTTGT+AGG 0.409408 3_1:-86019368 None:intergenic
GGAGGAACAACGACATCAAT+TGG 0.410263 3_1:-86018921 None:intergenic
TGCCCCTGCTGCAGCTGCCA+AGG 0.415195 3_1:+86019593 Msa0383240:CDS
TACCCTTGTTAATCTTGGTT+GGG 0.415505 3_1:-86019066 None:intergenic
CCTCCTTGGCAGCTGCAGCA+GGG 0.416133 3_1:-86019596 None:intergenic
ACAGCTACAGCAAACTTACT+TGG 0.421114 3_1:-86019540 None:intergenic
GACATTGGTTTCGGTCTGTT+TGG 0.422303 3_1:+86019657 Msa0383240:CDS
TCTGATGAAGACATTGGTTT+CGG 0.427250 3_1:+86019648 Msa0383240:CDS
CCAGCTGCTGCTGCGTCTGG+TGG 0.429030 3_1:+86019570 Msa0383240:CDS
GGGTCGAGCCCTGTGTTGCC+TGG 0.431244 3_1:-86018942 None:intergenic
CCTTTCTTAATGAGTTCCAC+AGG 0.432079 3_1:-86019106 None:intergenic
ACATTTCATGAGATCATACT+TGG 0.434128 3_1:+86019733 Msa0383240:three_prime_UTR
TGGTGACTCCGTTGTTCTCA+TGG 0.440885 3_1:+86017577 Msa0383240:CDS
GAGAGAGAGTGAGCGTGAAA+TGG 0.445704 3_1:-86017259 None:intergenic
TGCCTCAGGGAAATAATATT+CGG 0.446178 3_1:-86019401 None:intergenic
TCAGTGAGGTCAAGAACCTC+AGG 0.450763 3_1:-86019238 None:intergenic
AGAGTGAGCGTGAAATGGTT+GGG 0.451243 3_1:-86017254 None:intergenic
GACCACAAGCCCGTATGAAA+AGG 0.451799 3_1:-86019182 None:intergenic
AGCGTTGACAACAAGGATTT+GGG 0.451933 3_1:-86017507 None:intergenic
GAGAGTGAGCGTGAAATGGT+TGG 0.454050 3_1:-86017255 None:intergenic
AGAAAACTAACGACAAGAAG+AGG 0.456056 3_1:-86017677 None:intergenic
TGCTGCAGCTGCCAAGGAGG+AGG 0.457641 3_1:+86019599 Msa0383240:CDS
AAGGAACGAAACGAAGGGCT+TGG 0.460381 3_1:-86017292 None:intergenic
GCGTGAAATGGTTGGGAAGC+GGG 0.460938 3_1:-86017247 None:intergenic
ATTTAAGAGAGGAAATTAAA+GGG 0.465157 3_1:-86017374 None:intergenic
CCCTGAGGCAGACAAGGTTA+AGG 0.465890 3_1:+86019414 Msa0383240:CDS
GATCATACTTGGTTCATTCT+CGG 0.466035 3_1:+86019744 Msa0383240:three_prime_UTR
CGAAACGAAGGGCTTGGGTT+CGG 0.468578 3_1:-86017286 None:intergenic
TTTCACTAAAGGTGACTTGA+AGG 0.469610 3_1:+86018538 Msa0383240:CDS
ATTGATGTCGTTGTTCCTCC+AGG 0.473267 3_1:+86018924 Msa0383240:CDS
TCAGCTACAGAACATTCGTC+AGG 0.474511 3_1:+86017547 Msa0383240:CDS
TGCCAAGGAGGAGGCAAAGA+AGG 0.475094 3_1:+86019608 Msa0383240:CDS
GAGGAGCAGCGGCAAGTGTT+GGG 0.477710 3_1:-86019330 None:intergenic
TGTAGCGAAGTTTGCTATTG+GGG 0.487853 3_1:+86019270 Msa0383240:CDS
AGGAACGAAACGAAGGGCTT+GGG 0.488081 3_1:-86017291 None:intergenic
TGAGGAGCAGCGGCAAGTGT+TGG 0.489867 3_1:-86019331 None:intergenic
TAGTCAAATTACTGGTGATT+CGG 0.490669 3_1:+86019896 Msa0383240:three_prime_UTR
CTTAGTTCAAAACTTCAGAA+TGG 0.491518 3_1:-86017068 None:intergenic
ACCAGACGCAGCAGCAGCTG+GGG 0.494428 3_1:-86019568 None:intergenic
GTCCTCTCTGTTTATGACAA+TGG 0.495884 3_1:+86019202 Msa0383240:CDS
GTTTGCTATTGGGGTTTCCA+TGG 0.497232 3_1:+86019279 Msa0383240:CDS
TGCTTGCCAAGTTAGGCATT+AGG 0.501788 3_1:+86019158 Msa0383240:CDS
CTTGACTGAACTATACGTCT+TGG 0.501972 3_1:+86017351 Msa0383240:five_prime_UTR
GGGTGTATTCATCGATAAGC+TGG 0.506884 3_1:-86017487 None:intergenic
GTACCCTTGTTAATCTTGGT+TGG 0.507788 3_1:-86019067 None:intergenic
AGTCAAACTATAACATGGAA+TGG 0.510064 3_1:-86019789 None:intergenic
GCAACCAAACCAACACGAGC+AGG 0.521300 3_1:-86018897 None:intergenic
CGTATGCACGCTGAGAAAAC+CGG 0.521724 3_1:+86017629 Msa0383240:CDS
AAAGGGCCTAATGCCTAACT+TGG 0.523198 3_1:-86019164 None:intergenic
TTTGGGAGTTAACTCTCAAT+CGG 0.526599 3_1:-86017045 None:intergenic
CCCTGCTGCAGCTGCCAAGG+AGG 0.530721 3_1:+86019596 Msa0383240:CDS
TGCTTCTTCTCGGCTTTGGT+AGG 0.531501 3_1:-86017443 None:intergenic
ACCACAAGCCCGTATGAAAA+GGG 0.531757 3_1:-86019181 None:intergenic
GTTGGAGCTCCTGCTCGTGT+TGG 0.532062 3_1:+86018888 Msa0383240:CDS
TAGGCATTGACGAACATATG+AGG 0.532355 3_1:-86019349 None:intergenic
CAATAGCAAACTTCGCTACA+AGG 0.533756 3_1:-86019267 None:intergenic
AGCGTGAAATGGTTGGGAAG+CGG 0.536246 3_1:-86017248 None:intergenic
TGGAAGAAAGAGGTCTGAGA+TGG 0.537477 3_1:-86018963 None:intergenic
AACGAAGGGCTTGGGTTCGG+TGG 0.553305 3_1:-86017283 None:intergenic
GTGCTGAATCAGGGAAATGT+TGG 0.555601 3_1:+86018509 Msa0383240:intron
TAACGACAAGAAGAGGGATA+AGG 0.557262 3_1:-86017670 None:intergenic
ATTAAGAAAGGAGACAAGGT+TGG 0.560082 3_1:+86019118 Msa0383240:CDS
CCTGTGGAACTCATTAAGAA+AGG 0.562398 3_1:+86019106 Msa0383240:CDS
GTTGTTCCTCCAGGCAACAC+AGG 0.564540 3_1:+86018933 Msa0383240:CDS
CATCCCAACCAAGATTAACA+AGG 0.566179 3_1:+86019063 Msa0383240:CDS
GAAGAGTCTGATGAAGACAT+TGG 0.571608 3_1:+86019642 Msa0383240:CDS
CTTTCTTAATGAGTTCCACA+GGG 0.574196 3_1:-86019105 None:intergenic
CGTTGTCAGCGTTGACAACA+AGG 0.575399 3_1:-86017514 None:intergenic
ATAACAAACCTGGAAGAAAG+AGG 0.579894 3_1:-86018973 None:intergenic
GGTGACTCCGTTGTTCTCAT+GGG 0.580224 3_1:+86017578 Msa0383240:CDS
ACGAACAGATCGCTTCATCA+TGG 0.583200 3_1:-86017609 None:intergenic
CCTTAACCTTGTCTGCCTCA+GGG 0.589860 3_1:-86019414 None:intergenic
GGAAGAAAGAGGTCTGAGAT+GGG 0.590223 3_1:-86018962 None:intergenic
CAAATTAAAAGTTAAAACCT+AGG 0.591614 3_1:-86019866 None:intergenic
CAGCTACAGCAAACTTACTT+GGG 0.593694 3_1:-86019539 None:intergenic
TTATTTCCCTGAGGCAGACA+AGG 0.600567 3_1:+86019408 Msa0383240:CDS
TTTGGAAGGAACGAAACGAA+GGG 0.600757 3_1:-86017297 None:intergenic
GGCAAGTGTTGGGTAAGAGA+TGG 0.602128 3_1:-86019320 None:intergenic
GAAAACTAACGACAAGAAGA+GGG 0.606607 3_1:-86017676 None:intergenic
GGGAGTTAACTCTCAATCGG+TGG 0.609159 3_1:-86017042 None:intergenic
AAAAGAGCAGGTTAAAGTGA+CGG 0.611034 3_1:+86017318 Msa0383240:five_prime_UTR
TTGTTCCTCCAGGCAACACA+GGG 0.611479 3_1:+86018934 Msa0383240:CDS
CAAGATTAACAAGGGTACTG+TGG 0.615021 3_1:+86019072 Msa0383240:CDS
AACAACAACAAAATCAACAA+TGG 0.626124 3_1:+86017412 Msa0383240:exon
TGAAGAAGTTGCTAAATACA+AGG 0.626443 3_1:+86018568 Msa0383240:CDS
GACGAACATATGAGGAGCAG+CGG 0.628131 3_1:-86019341 None:intergenic
CAGCTACAGAACATTCGTCA+GGG 0.630095 3_1:+86017548 Msa0383240:CDS
GTTGTCAACGCTGACAACGT+TGG 0.630374 3_1:+86017518 Msa0383240:CDS
TGGTTCAGTGTTTAGCCCTG+AGG 0.633517 3_1:+86019222 Msa0383240:CDS
ATTAAAAGTTAAAACCTAGG+AGG 0.636784 3_1:-86019863 None:intergenic
GGGGCCGTGAGCAAAAGAAG+GGG 0.640653 3_1:+86017114 Msa0383240:five_prime_UTR
GATAAAGTCAAACTATAACA+TGG 0.656378 3_1:-86019794 None:intergenic
CAATGAAAGTGAAGTGACCA+TGG 0.657879 3_1:-86019296 None:intergenic
CAAGGTTAAGGAGTACCTGA+AGG 0.661074 3_1:+86019426 Msa0383240:CDS
CAAGACGTATAGTTCAGTCA+AGG 0.663107 3_1:-86017350 None:intergenic
GTTCTTTCCCATGAGAACAA+CGG 0.664224 3_1:-86017585 None:intergenic
ATCCCAACCAAGATTAACAA+GGG 0.664581 3_1:+86019064 Msa0383240:CDS
AACATTCGTCAGGGTCTACG+TGG 0.667957 3_1:+86017557 Msa0383240:CDS
TGTGGAAATTATTACCCCTG+TGG 0.672323 3_1:+86019090 Msa0383240:CDS
ACTCATTAAGAAAGGAGACA+AGG 0.674494 3_1:+86019114 Msa0383240:CDS
TCGAGCCCTGTGTTGCCTGG+AGG 0.687408 3_1:-86018939 None:intergenic
CAGTGAGGTCAAGAACCTCA+GGG 0.701495 3_1:-86019237 None:intergenic
TGAGGTTCTTGACCTCACTG+AGG 0.710337 3_1:+86019240 Msa0383240:CDS
AAACACTTAGCAAACCCAGT+GGG 0.712309 3_1:+86017183 Msa0383240:five_prime_UTR
AACCATTGTCATAAACAGAG+AGG 0.717707 3_1:-86019204 None:intergenic
GACGTATAGTTCAGTCAAGG+CGG 0.717823 3_1:-86017347 None:intergenic
TACCGAATATTATTTCCCTG+AGG 0.725295 3_1:+86019399 Msa0383240:CDS
TTTCTTAATGAGTTCCACAG+GGG 0.725748 3_1:-86019104 None:intergenic
CTACAAGGTCATCCTCAGTG+AGG 0.743500 3_1:-86019252 None:intergenic
AAAACACTTAGCAAACCCAG+TGG 0.777128 3_1:+86017182 Msa0383240:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTAAAAAAAAAAAAAAA+AGG + chr3_1:86019956-86019975 Msa0383240:three_prime_UTR 0.0%
!!! AGTCTAACTTTATTTATAAT+GGG + chr3_1:86017897-86017916 Msa0383240:intron 15.0%
!! TATTATTTATAAGCTTATCA+AGG - chr3_1:86018042-86018061 None:intergenic 15.0%
!! TAAGCTTATAAATAATACAT+AGG + chr3_1:86018045-86018064 Msa0383240:intron 15.0%
!! AAGCTTATAAATAATACATA+GGG + chr3_1:86018046-86018065 Msa0383240:intron 15.0%
!! AATCAAAGAATAAGAAAAAT+GGG - chr3_1:86019503-86019522 None:intergenic 15.0%
!! AAATCAAAGAATAAGAAAAA+TGG - chr3_1:86019504-86019523 None:intergenic 15.0%
!! ATTTAAGAGAGGAAATTAAA+GGG - chr3_1:86017377-86017396 None:intergenic 20.0%
!!! CAGTCTAACTTTATTTATAA+TGG + chr3_1:86017896-86017915 Msa0383240:intron 20.0%
!!! GTCTAACTTTATTTATAATG+GGG + chr3_1:86017898-86017917 Msa0383240:intron 20.0%
!! TATGCAAATAAATAAGCTTA+TGG - chr3_1:86018275-86018294 None:intergenic 20.0%
!! TAATTGTCTTAAATCTGAAT+AGG + chr3_1:86018866-86018885 Msa0383240:intron 20.0%
!!! TTCTCGTTTTAAATTCTATA+TGG + chr3_1:86019822-86019841 Msa0383240:three_prime_UTR 20.0%
!! CAAATTAAAAGTTAAAACCT+AGG - chr3_1:86019869-86019888 None:intergenic 20.0%
!!! TTTAACTTTTAATTTGCGTT+CGG + chr3_1:86019873-86019892 Msa0383240:three_prime_UTR 20.0%
!! TTGAACTAAGATAGGTATTT+TGG + chr3_1:86017081-86017100 Msa0383240:five_prime_UTR 25.0%
!! TGAACTAAGATAGGTATTTT+GGG + chr3_1:86017082-86017101 Msa0383240:five_prime_UTR 25.0%
! GATTTAAGAGAGGAAATTAA+AGG - chr3_1:86017378-86017397 None:intergenic 25.0%
!!! TGATTTTGTTGTTGTTGATA+GGG - chr3_1:86017408-86017427 None:intergenic 25.0%
!!! TTGATTTTGTTGTTGTTGAT+AGG - chr3_1:86017409-86017428 None:intergenic 25.0%
! AACAACAACAAAATCAACAA+TGG + chr3_1:86017412-86017431 Msa0383240:exon 25.0%
!! TTTAGTGATTTTGTGTGATT+TGG + chr3_1:86017752-86017771 Msa0383240:intron 25.0%
!!! TTCTGTTTTTTGTTACGTAT+AGG + chr3_1:86017788-86017807 Msa0383240:intron 25.0%
! TCTTTATGTTAATTCACAAG+TGG + chr3_1:86017974-86017993 Msa0383240:intron 25.0%
! CTTTATGTTAATTCACAAGT+GGG + chr3_1:86017975-86017994 Msa0383240:intron 25.0%
! TAGTGAGAACTTATGAATTA+AGG + chr3_1:86018184-86018203 Msa0383240:intron 25.0%
! AATTACTGCACTATATTATG+AGG + chr3_1:86018450-86018469 Msa0383240:intron 25.0%
! ATATGTTATGTTGTTAAGCA+TGG + chr3_1:86018615-86018634 Msa0383240:intron 25.0%
! ATTTACAGAAATTGTAACGA+GGG - chr3_1:86018760-86018779 None:intergenic 25.0%
! TATTTACAGAAATTGTAACG+AGG - chr3_1:86018761-86018780 None:intergenic 25.0%
! TTAAAAGAAGATAACAAACC+TGG - chr3_1:86018986-86019005 None:intergenic 25.0%
! TTATGTTTATTCTCCACTTT+AGG + chr3_1:86019033-86019052 Msa0383240:intron 25.0%
!!! TTCTTTAGTTTTGCTATTAG+TGG + chr3_1:86019691-86019710 Msa0383240:three_prime_UTR 25.0%
! GATAAAGTCAAACTATAACA+TGG - chr3_1:86019797-86019816 None:intergenic 25.0%
! ATTAAAAGTTAAAACCTAGG+AGG - chr3_1:86019866-86019885 None:intergenic 25.0%
TCAAAACTTCAGAATGGTTT+GGG - chr3_1:86017065-86017084 None:intergenic 30.0%
TTCAAAACTTCAGAATGGTT+TGG - chr3_1:86017066-86017085 None:intergenic 30.0%
CTTAGTTCAAAACTTCAGAA+TGG - chr3_1:86017071-86017090 None:intergenic 30.0%
!!! CTGAAGTTTTGAACTAAGAT+AGG + chr3_1:86017073-86017092 Msa0383240:five_prime_UTR 30.0%
! GAACTAAGATAGGTATTTTG+GGG + chr3_1:86017083-86017102 Msa0383240:five_prime_UTR 30.0%
! GGCGCAAAGATTTTATATTA+GGG - chr3_1:86017229-86017248 None:intergenic 30.0%
TTCGCTGTTCGATTTAATAA+TGG + chr3_1:86017719-86017738 Msa0383240:intron 30.0%
AATAATACATAGGGTCTGTT+TGG + chr3_1:86018055-86018074 Msa0383240:intron 30.0%
CAAAATAAGCTCAATCCAAA+CGG + chr3_1:86018247-86018266 Msa0383240:intron 30.0%
AAAATAAGCTCAATCCAAAC+GGG + chr3_1:86018248-86018267 Msa0383240:intron 30.0%
TTTAGCTGTCTATACAAACA+AGG + chr3_1:86018379-86018398 Msa0383240:intron 30.0%
! TATTGTTGTTAACTCTTGTG+AGG + chr3_1:86018482-86018501 Msa0383240:intron 30.0%
!!! GTTGGTTTGATTTTCACTAA+AGG + chr3_1:86018527-86018546 Msa0383240:CDS 30.0%
TGAAGAAGTTGCTAAATACA+AGG + chr3_1:86018568-86018587 Msa0383240:CDS 30.0%
! AATACTTTTCGTTTGATGCA+TGG + chr3_1:86018780-86018799 Msa0383240:intron 30.0%
TGTCTTAAATCTGAATAGGT+TGG + chr3_1:86018870-86018889 Msa0383240:intron 30.0%
ACATTTCATGAGATCATACT+TGG + chr3_1:86019733-86019752 Msa0383240:three_prime_UTR 30.0%
AGTCAAACTATAACATGGAA+TGG - chr3_1:86019792-86019811 None:intergenic 30.0%
TAGTCAAATTACTGGTGATT+CGG + chr3_1:86019896-86019915 Msa0383240:three_prime_UTR 30.0%
TTTGGGAGTTAACTCTCAAT+CGG - chr3_1:86017048-86017067 None:intergenic 35.0%
! GGGCGCAAAGATTTTATATT+AGG - chr3_1:86017230-86017249 None:intergenic 35.0%
! TTTAACCTGCTCTTTTTGGA+AGG - chr3_1:86017314-86017333 None:intergenic 35.0%
! TCACTTTAACCTGCTCTTTT+TGG - chr3_1:86017318-86017337 None:intergenic 35.0%
AAAAGAGCAGGTTAAAGTGA+CGG + chr3_1:86017318-86017337 Msa0383240:five_prime_UTR 35.0%
!!! GGGTTTTGAAGATTTAAGAG+AGG - chr3_1:86017388-86017407 None:intergenic 35.0%
GAAAACTAACGACAAGAAGA+GGG - chr3_1:86017679-86017698 None:intergenic 35.0%
AGAAAACTAACGACAAGAAG+AGG - chr3_1:86017680-86017699 None:intergenic 35.0%
AAATAAGCTCAATCCAAACG+GGG + chr3_1:86018249-86018268 Msa0383240:intron 35.0%
TTTCACTAAAGGTGACTTGA+AGG + chr3_1:86018538-86018557 Msa0383240:CDS 35.0%
CATTCAACAAACAAGACAAG+TGG - chr3_1:86018833-86018852 None:intergenic 35.0%
ATAACAAACCTGGAAGAAAG+AGG - chr3_1:86018976-86018995 None:intergenic 35.0%
ATCCCAACCAAGATTAACAA+GGG + chr3_1:86019064-86019083 Msa0383240:CDS 35.0%
TACCCTTGTTAATCTTGGTT+GGG - chr3_1:86019069-86019088 None:intergenic 35.0%
TTTCTTAATGAGTTCCACAG+GGG - chr3_1:86019107-86019126 None:intergenic 35.0%
CTTTCTTAATGAGTTCCACA+GGG - chr3_1:86019108-86019127 None:intergenic 35.0%
ACTCATTAAGAAAGGAGACA+AGG + chr3_1:86019114-86019133 Msa0383240:CDS 35.0%
ATTAAGAAAGGAGACAAGGT+TGG + chr3_1:86019118-86019137 Msa0383240:CDS 35.0%
AACCATTGTCATAAACAGAG+AGG - chr3_1:86019207-86019226 None:intergenic 35.0%
!! TTGTAGCGAAGTTTGCTATT+GGG + chr3_1:86019269-86019288 Msa0383240:CDS 35.0%
AAAAGCAAGAACGTTCTTGT+AGG - chr3_1:86019371-86019390 None:intergenic 35.0%
TACCGAATATTATTTCCCTG+AGG + chr3_1:86019399-86019418 Msa0383240:CDS 35.0%
TGCCTCAGGGAAATAATATT+CGG - chr3_1:86019404-86019423 None:intergenic 35.0%
ATGTTGAAGTCAATACCTTC+AGG - chr3_1:86019444-86019463 None:intergenic 35.0%
!! CTTTGATTTTTGTTCACTGC+AGG + chr3_1:86019515-86019534 Msa0383240:intron 35.0%
!! TCTGATGAAGACATTGGTTT+CGG + chr3_1:86019648-86019667 Msa0383240:CDS 35.0%
GATCATACTTGGTTCATTCT+CGG + chr3_1:86019744-86019763 Msa0383240:three_prime_UTR 35.0%
!! TAGGTATTTTGGGGAGTTTG+AGG + chr3_1:86017092-86017111 Msa0383240:five_prime_UTR 40.0%
!! AGGTATTTTGGGGAGTTTGA+GGG + chr3_1:86017093-86017112 Msa0383240:five_prime_UTR 40.0%
AAAACACTTAGCAAACCCAG+TGG + chr3_1:86017182-86017201 Msa0383240:five_prime_UTR 40.0%
AAACACTTAGCAAACCCAGT+GGG + chr3_1:86017183-86017202 Msa0383240:five_prime_UTR 40.0%
TTTGGAAGGAACGAAACGAA+GGG - chr3_1:86017300-86017319 None:intergenic 40.0%
!! TTTTGGAAGGAACGAAACGA+AGG - chr3_1:86017301-86017320 None:intergenic 40.0%
CAAGACGTATAGTTCAGTCA+AGG - chr3_1:86017353-86017372 None:intergenic 40.0%
CTTGACTGAACTATACGTCT+TGG + chr3_1:86017351-86017370 Msa0383240:five_prime_UTR 40.0%
! ATCGTAGTTCTGCTTCTTCT+CGG - chr3_1:86017456-86017475 None:intergenic 40.0%
!! AGCGTTGACAACAAGGATTT+GGG - chr3_1:86017510-86017529 None:intergenic 40.0%
GTTCTTTCCCATGAGAACAA+CGG - chr3_1:86017588-86017607 None:intergenic 40.0%
!! GTTGAGAAAAGCATTGTTGC+CGG - chr3_1:86017651-86017670 None:intergenic 40.0%
TAACGACAAGAAGAGGGATA+AGG - chr3_1:86017673-86017692 None:intergenic 40.0%
! ATAATGGGGCGTGTTTGAAT+TGG + chr3_1:86017912-86017931 Msa0383240:intron 40.0%
TGAGGATTTGTGCTGAATCA+GGG + chr3_1:86018500-86018519 Msa0383240:intron 40.0%
! CACTAGAGGTGCAACAAAAA+TGG - chr3_1:86018687-86018706 None:intergenic 40.0%
! TTTTTGTTGCACCTCTAGTG+AGG + chr3_1:86018687-86018706 Msa0383240:intron 40.0%
CATCCCAACCAAGATTAACA+AGG + chr3_1:86019063-86019082 Msa0383240:CDS 40.0%
GTACCCTTGTTAATCTTGGT+TGG - chr3_1:86019070-86019089 None:intergenic 40.0%
CACAGTACCCTTGTTAATCT+TGG - chr3_1:86019074-86019093 None:intergenic 40.0%
!! CAAGATTAACAAGGGTACTG+TGG + chr3_1:86019072-86019091 Msa0383240:CDS 40.0%
TGTGGAAATTATTACCCCTG+TGG + chr3_1:86019090-86019109 Msa0383240:CDS 40.0%
CCTTTCTTAATGAGTTCCAC+AGG - chr3_1:86019109-86019128 None:intergenic 40.0%
CCTGTGGAACTCATTAAGAA+AGG + chr3_1:86019106-86019125 Msa0383240:CDS 40.0%
GTCCTCTCTGTTTATGACAA+TGG + chr3_1:86019202-86019221 Msa0383240:CDS 40.0%
CAATAGCAAACTTCGCTACA+AGG - chr3_1:86019270-86019289 None:intergenic 40.0%
!! CTTGTAGCGAAGTTTGCTAT+TGG + chr3_1:86019268-86019287 Msa0383240:CDS 40.0%
!! TGTAGCGAAGTTTGCTATTG+GGG + chr3_1:86019270-86019289 Msa0383240:CDS 40.0%
CAATGAAAGTGAAGTGACCA+TGG - chr3_1:86019299-86019318 None:intergenic 40.0%
CATGGTCACTTCACTTTCAT+TGG + chr3_1:86019297-86019316 Msa0383240:CDS 40.0%
!! TAGGCATTGACGAACATATG+AGG - chr3_1:86019352-86019371 None:intergenic 40.0%
CAGCTACAGCAAACTTACTT+GGG - chr3_1:86019542-86019561 None:intergenic 40.0%
ACAGCTACAGCAAACTTACT+TGG - chr3_1:86019543-86019562 None:intergenic 40.0%
TCTTCATCAGACTCTTCTTC+AGG - chr3_1:86019639-86019658 None:intergenic 40.0%
GAAGAGTCTGATGAAGACAT+TGG + chr3_1:86019642-86019661 Msa0383240:CDS 40.0%
!! GGTATTTTGGGGAGTTTGAG+GGG + chr3_1:86017094-86017113 Msa0383240:five_prime_UTR 45.0%
!! GTATTTTGGGGAGTTTGAGG+GGG + chr3_1:86017095-86017114 Msa0383240:five_prime_UTR 45.0%
! TAAGCCCCTTCTTTTGCTCA+CGG - chr3_1:86017121-86017140 None:intergenic 45.0%
AAACCCAGTGGGCCTAAAAA+GGG + chr3_1:86017194-86017213 Msa0383240:five_prime_UTR 45.0%
! GGGTTTTCTCTGCCCTTTTT+AGG - chr3_1:86017209-86017228 None:intergenic 45.0%
AGAGTGAGCGTGAAATGGTT+GGG - chr3_1:86017257-86017276 None:intergenic 45.0%
TCGTTCCTTCCAAAAAGAGC+AGG + chr3_1:86017306-86017325 Msa0383240:five_prime_UTR 45.0%
GACGTATAGTTCAGTCAAGG+CGG - chr3_1:86017350-86017369 None:intergenic 45.0%
GGGTGTATTCATCGATAAGC+TGG - chr3_1:86017490-86017509 None:intergenic 45.0%
!! CAGCGTTGACAACAAGGATT+TGG - chr3_1:86017511-86017530 None:intergenic 45.0%
TCAGCTACAGAACATTCGTC+AGG + chr3_1:86017547-86017566 Msa0383240:CDS 45.0%
CAGCTACAGAACATTCGTCA+GGG + chr3_1:86017548-86017567 Msa0383240:CDS 45.0%
ACGAACAGATCGCTTCATCA+TGG - chr3_1:86017612-86017631 None:intergenic 45.0%
AATAAGCTTATGGCCCCGTT+TGG - chr3_1:86018265-86018284 None:intergenic 45.0%
GTGAGGATTTGTGCTGAATC+AGG + chr3_1:86018499-86018518 Msa0383240:intron 45.0%
GTGCTGAATCAGGGAAATGT+TGG + chr3_1:86018509-86018528 Msa0383240:intron 45.0%
GGAGGAACAACGACATCAAT+TGG - chr3_1:86018924-86018943 None:intergenic 45.0%
ATTGATGTCGTTGTTCCTCC+AGG + chr3_1:86018924-86018943 Msa0383240:CDS 45.0%
! GGAAGAAAGAGGTCTGAGAT+GGG - chr3_1:86018965-86018984 None:intergenic 45.0%
TGGAAGAAAGAGGTCTGAGA+TGG - chr3_1:86018966-86018985 None:intergenic 45.0%
ATCTCAGACCTCTTTCTTCC+AGG + chr3_1:86018965-86018984 Msa0383240:CDS 45.0%
TGCTTGCCAAGTTAGGCATT+AGG + chr3_1:86019158-86019177 Msa0383240:CDS 45.0%
AAAGGGCCTAATGCCTAACT+TGG - chr3_1:86019167-86019186 None:intergenic 45.0%
! GGCATTAGGCCCTTTTCATA+CGG + chr3_1:86019172-86019191 Msa0383240:CDS 45.0%
! GCATTAGGCCCTTTTCATAC+GGG + chr3_1:86019173-86019192 Msa0383240:CDS 45.0%
ACCACAAGCCCGTATGAAAA+GGG - chr3_1:86019184-86019203 None:intergenic 45.0%
!! GTTTGCTATTGGGGTTTCCA+TGG + chr3_1:86019279-86019298 Msa0383240:CDS 45.0%
TTATTTCCCTGAGGCAGACA+AGG + chr3_1:86019408-86019427 Msa0383240:CDS 45.0%
!! CAAGGTTAAGGAGTACCTGA+AGG + chr3_1:86019426-86019445 Msa0383240:CDS 45.0%
!! GACATTGGTTTCGGTCTGTT+TGG + chr3_1:86019657-86019676 Msa0383240:CDS 45.0%
GCGTTCGGTAGTCAAATTAC+TGG + chr3_1:86019888-86019907 Msa0383240:three_prime_UTR 45.0%
!! AACTTATAAATAATAAATAA+AGG - chr3_1:86018116-86018135 None:intergenic 5.0%
GGGAGTTAACTCTCAATCGG+TGG - chr3_1:86017045-86017064 None:intergenic 50.0%
CAAACCCAGTGGGCCTAAAA+AGG + chr3_1:86017193-86017212 Msa0383240:five_prime_UTR 50.0%
AGCGTGAAATGGTTGGGAAG+CGG - chr3_1:86017251-86017270 None:intergenic 50.0%
GAGAGTGAGCGTGAAATGGT+TGG - chr3_1:86017258-86017277 None:intergenic 50.0%
GAGAGAGAGTGAGCGTGAAA+TGG - chr3_1:86017262-86017281 None:intergenic 50.0%
AGGAACGAAACGAAGGGCTT+GGG - chr3_1:86017294-86017313 None:intergenic 50.0%
AAGGAACGAAACGAAGGGCT+TGG - chr3_1:86017295-86017314 None:intergenic 50.0%
! TGCTTCTTCTCGGCTTTGGT+AGG - chr3_1:86017446-86017465 None:intergenic 50.0%
GTTCTGCTTCTTCTCGGCTT+TGG - chr3_1:86017450-86017469 None:intergenic 50.0%
!! CGTTGTCAGCGTTGACAACA+AGG - chr3_1:86017517-86017536 None:intergenic 50.0%
GTTGTCAACGCTGACAACGT+TGG + chr3_1:86017518-86017537 Msa0383240:CDS 50.0%
AACATTCGTCAGGGTCTACG+TGG + chr3_1:86017557-86017576 Msa0383240:CDS 50.0%
TGGTGACTCCGTTGTTCTCA+TGG + chr3_1:86017577-86017596 Msa0383240:CDS 50.0%
GGTGACTCCGTTGTTCTCAT+GGG + chr3_1:86017578-86017597 Msa0383240:CDS 50.0%
CGTATGCACGCTGAGAAAAC+CGG + chr3_1:86017629-86017648 Msa0383240:CDS 50.0%
CAACCACAGTACCTCACTAG+AGG - chr3_1:86018701-86018720 None:intergenic 50.0%
GCATGGCAATTGCAGCAATG+TGG + chr3_1:86018797-86018816 Msa0383240:intron 50.0%
TTGTTCCTCCAGGCAACACA+GGG + chr3_1:86018934-86018953 Msa0383240:CDS 50.0%
GGGATGTTGAGCACCTAAAG+TGG - chr3_1:86019049-86019068 None:intergenic 50.0%
GCTGCATTGCTTGCCAAGTT+AGG + chr3_1:86019151-86019170 Msa0383240:CDS 50.0%
GACCACAAGCCCGTATGAAA+AGG - chr3_1:86019185-86019204 None:intergenic 50.0%
TGGTTCAGTGTTTAGCCCTG+AGG + chr3_1:86019222-86019241 Msa0383240:CDS 50.0%
CAGTGAGGTCAAGAACCTCA+GGG - chr3_1:86019240-86019259 None:intergenic 50.0%
TCAGTGAGGTCAAGAACCTC+AGG - chr3_1:86019241-86019260 None:intergenic 50.0%
TGAGGTTCTTGACCTCACTG+AGG + chr3_1:86019240-86019259 Msa0383240:CDS 50.0%
CTACAAGGTCATCCTCAGTG+AGG - chr3_1:86019255-86019274 None:intergenic 50.0%
!! GGCAAGTGTTGGGTAAGAGA+TGG - chr3_1:86019323-86019342 None:intergenic 50.0%
GACGAACATATGAGGAGCAG+CGG - chr3_1:86019344-86019363 None:intergenic 50.0%
CCTTAACCTTGTCTGCCTCA+GGG - chr3_1:86019417-86019436 None:intergenic 50.0%
TCCTTAACCTTGTCTGCCTC+AGG - chr3_1:86019418-86019437 None:intergenic 50.0%
TTCCTTCTTTGCCTCCTCCT+TGG - chr3_1:86019613-86019632 None:intergenic 50.0%
! GTGACTTGTGTTTGCCTCCT+AGG + chr3_1:86019849-86019868 Msa0383240:three_prime_UTR 50.0%
!! CTGCCCTTTTTAGGCCCACT+GGG - chr3_1:86017200-86017219 None:intergenic 55.0%
!! TCTGCCCTTTTTAGGCCCAC+TGG - chr3_1:86017201-86017220 None:intergenic 55.0%
GCGTGAAATGGTTGGGAAGC+GGG - chr3_1:86017250-86017269 None:intergenic 55.0%
CGAAACGAAGGGCTTGGGTT+CGG - chr3_1:86017289-86017308 None:intergenic 55.0%
CTGCGTACGGGCGTTAGAAT+TGG - chr3_1:86018326-86018345 None:intergenic 55.0%
!! GCACCTCTAGTGAGGTACTG+TGG + chr3_1:86018695-86018714 Msa0383240:intron 55.0%
!! AGCTCCTGCTCGTGTTGGTT+TGG + chr3_1:86018893-86018912 Msa0383240:CDS 55.0%
GCAACCAAACCAACACGAGC+AGG - chr3_1:86018900-86018919 None:intergenic 55.0%
GTTGTTCCTCCAGGCAACAC+AGG + chr3_1:86018933-86018952 Msa0383240:CDS 55.0%
! GCCCTTTTCATACGGGCTTG+TGG + chr3_1:86019180-86019199 Msa0383240:CDS 55.0%
CCCTGAGGCAGACAAGGTTA+AGG + chr3_1:86019414-86019433 Msa0383240:CDS 55.0%
TGCCAAGGAGGAGGCAAAGA+AGG + chr3_1:86019608-86019627 Msa0383240:CDS 55.0%
AGGGGGCCGTGAGCAAAAGA+AGG + chr3_1:86017112-86017131 Msa0383240:five_prime_UTR 60.0%
GGGGGCCGTGAGCAAAAGAA+GGG + chr3_1:86017113-86017132 Msa0383240:five_prime_UTR 60.0%
GGGGCCGTGAGCAAAAGAAG+GGG + chr3_1:86017114-86017133 Msa0383240:five_prime_UTR 60.0%
AACGAAGGGCTTGGGTTCGG+TGG - chr3_1:86017286-86017305 None:intergenic 60.0%
AAGCGCGTGCTCACTGCGTA+CGG - chr3_1:86018339-86018358 None:intergenic 60.0%
GTTGGAGCTCCTGCTCGTGT+TGG + chr3_1:86018888-86018907 Msa0383240:CDS 60.0%
GAGGAGCAGCGGCAAGTGTT+GGG - chr3_1:86019333-86019352 None:intergenic 60.0%
TGAGGAGCAGCGGCAAGTGT+TGG - chr3_1:86019334-86019353 None:intergenic 60.0%
AGCGCGTGCTCACTGCGTAC+GGG - chr3_1:86018338-86018357 None:intergenic 65.0%
TCGAGCCCTGTGTTGCCTGG+AGG - chr3_1:86018942-86018961 None:intergenic 65.0%
ACCAGACGCAGCAGCAGCTG+GGG - chr3_1:86019571-86019590 None:intergenic 65.0%
CACCAGACGCAGCAGCAGCT+GGG - chr3_1:86019572-86019591 None:intergenic 65.0%
CTCCTTGGCAGCTGCAGCAG+GGG - chr3_1:86019598-86019617 None:intergenic 65.0%
CCTCCTTGGCAGCTGCAGCA+GGG - chr3_1:86019599-86019618 None:intergenic 65.0%
TCCTCCTTGGCAGCTGCAGC+AGG - chr3_1:86019600-86019619 None:intergenic 65.0%
TGCTGCAGCTGCCAAGGAGG+AGG + chr3_1:86019599-86019618 Msa0383240:CDS 65.0%
GGGTCGAGCCCTGTGTTGCC+TGG - chr3_1:86018945-86018964 None:intergenic 70.0%
CCACCAGACGCAGCAGCAGC+TGG - chr3_1:86019573-86019592 None:intergenic 70.0%
CCAGCTGCTGCTGCGTCTGG+TGG + chr3_1:86019570-86019589 Msa0383240:CDS 70.0%
TGCCCCTGCTGCAGCTGCCA+AGG + chr3_1:86019593-86019612 Msa0383240:CDS 70.0%
CCCTGCTGCAGCTGCCAAGG+AGG + chr3_1:86019596-86019615 Msa0383240:CDS 70.0%
GCCCCAGCTGCTGCTGCGTC+TGG + chr3_1:86019567-86019586 Msa0383240:CDS 75.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 86017039 86019982 86017039 ID=Msa0383240;Name=Msa0383240
chr3_1 mRNA 86017039 86019982 86017039 ID=Msa0383240-mRNA-1;Parent=Msa0383240;Name=Msa0383240-mRNA-1;_AED=0.02;_eAED=0.02;_QI=392|1|1|1|1|1|5|293|323
chr3_1 exon 86017039 86017688 86017039 ID=Msa0383240-mRNA-1:exon:22704;Parent=Msa0383240-mRNA-1
chr3_1 exon 86018521 86018589 86018521 ID=Msa0383240-mRNA-1:exon:22705;Parent=Msa0383240-mRNA-1
chr3_1 exon 86018888 86018986 86018888 ID=Msa0383240-mRNA-1:exon:22706;Parent=Msa0383240-mRNA-1
chr3_1 exon 86019055 86019447 86019055 ID=Msa0383240-mRNA-1:exon:22707;Parent=Msa0383240-mRNA-1
chr3_1 exon 86019537 86019982 86019537 ID=Msa0383240-mRNA-1:exon:22708;Parent=Msa0383240-mRNA-1
chr3_1 five_prime_UTR 86017039 86017430 86017039 ID=Msa0383240-mRNA-1:five_prime_utr;Parent=Msa0383240-mRNA-1
chr3_1 CDS 86017431 86017688 86017431 ID=Msa0383240-mRNA-1:cds;Parent=Msa0383240-mRNA-1
chr3_1 CDS 86018521 86018589 86018521 ID=Msa0383240-mRNA-1:cds;Parent=Msa0383240-mRNA-1
chr3_1 CDS 86018888 86018986 86018888 ID=Msa0383240-mRNA-1:cds;Parent=Msa0383240-mRNA-1
chr3_1 CDS 86019055 86019447 86019055 ID=Msa0383240-mRNA-1:cds;Parent=Msa0383240-mRNA-1
chr3_1 CDS 86019537 86019689 86019537 ID=Msa0383240-mRNA-1:cds;Parent=Msa0383240-mRNA-1
chr3_1 three_prime_UTR 86019690 86019982 86019690 ID=Msa0383240-mRNA-1:three_prime_utr;Parent=Msa0383240-mRNA-1
Gene Sequence

>Msa0383240

ATGGCAGTGAAACCTACCAAAGCCGAGAAGAAGCAGAACTACGATGCTAAGCTTTGCCAGCTTATCGATGAATACACCCAAATCCTTGTTGTCAACGCTGACAACGTTGGATCTAATCAGCTACAGAACATTCGTCAGGGTCTACGTGGTGACTCCGTTGTTCTCATGGGAAAGAACACCATGATGAAGCGATCTGTTCGTATGCACGCTGAGAAAACCGGCAACAATGCTTTTCTCAACCTTATCCCTCTTCTTGTCGGAAATGTTGGTTTGATTTTCACTAAAGGTGACTTGAAGGAAGTGAGTGAAGAAGTTGCTAAATACAAGGTTGGAGCTCCTGCTCGTGTTGGTTTGGTTGCTCCAATTGATGTCGTTGTTCCTCCAGGCAACACAGGGCTCGACCCATCTCAGACCTCTTTCTTCCAGGTGCTCAACATCCCAACCAAGATTAACAAGGGTACTGTGGAAATTATTACCCCTGTGGAACTCATTAAGAAAGGAGACAAGGTTGGATCTTCTGAAGCTGCATTGCTTGCCAAGTTAGGCATTAGGCCCTTTTCATACGGGCTTGTGGTCCTCTCTGTTTATGACAATGGTTCAGTGTTTAGCCCTGAGGTTCTTGACCTCACTGAGGATGACCTTGTAGCGAAGTTTGCTATTGGGGTTTCCATGGTCACTTCACTTTCATTGGCCATCTCTTACCCAACACTTGCCGCTGCTCCTCATATGTTCGTCAATGCCTACAAGAACGTTCTTGCTTTTGCAGTTGCTACCGAATATTATTTCCCTGAGGCAGACAAGGTTAAGGAGTACCTGAAGGACCCAAGTAAGTTTGCTGTAGCTGTTGCTGCCCCAGCTGCTGCTGCGTCTGGTGGTGCCCCTGCTGCAGCTGCCAAGGAGGAGGCAAAGAAGGAAGAGCCTGAAGAAGAGTCTGATGAAGACATTGGTTTCGGTCTGTTTGGTGATGATTAG
Protein sequence

>Msa0383240

MAVKPTKAEKKQNYDAKLCQLIDEYTQILVVNADNVGSNQLQNIRQGLRGDSVVLMGKNTMMKRSVRMHAEKTGNNAFLNLIPLLVGNVGLIFTKGDLKEVSEEVAKYKVGAPARVGLVAPIDVVVPPGNTGLDPSQTSFFQVLNIPTKINKGTVEIITPVELIKKGDKVGSSEAALLAKLGIRPFSYGLVVLSVYDNGSVFSPEVLDLTEDDLVAKFAIGVSMVTSLSLAISYPTLAAAPHMFVNAYKNVLAFAVATEYYFPEADKVKEYLKDPSKFAVAVAAPAAAASGGAPAAAAKEEAKKEEPEEESDEDIGFGLFGDD*