AlfalfaGEDB | Msa0127230

M. sativa cultivar zhongmu-4 / Msa0127230

KEGG: K02873
GO: -
PFAM: PF01294.21

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0127230	sp\|P41129\|RL132_BRANA	85.437	206	30	0	2	207	1	206	2.68e-130	367

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0127230	B7FGS0	100.000	207	0	0	1	207	1	207	1.20e-148	420

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0003030	Msa0127230	0.853817	1.859007e-61	-8.615850e-47
Msa0003330	Msa0127230	0.825826	3.792962e-54	-8.615850e-47
Msa0026270	Msa0127230	0.819288	1.256458e-52	-8.615850e-47
Msa0042510	Msa0127230	0.992059	4.535867e-191	-8.615850e-47
Msa0079560	Msa0127230	0.998228	2.505728e-259	-8.615850e-47
Msa0086160	Msa0127230	0.841540	4.466219e-58	-8.615850e-47
Msa0116600	Msa0127230	0.819747	9.875690e-53	-8.615850e-47
Msa0116690	Msa0127230	0.805573	1.250340e-49	-8.615850e-47
Msa0127230	Msa0146910	0.851712	7.425426e-61	-8.615850e-47
Msa0127230	Msa0167860	0.813754	2.180902e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0127230	MtrunA17_Chr1g0155011	100.000	207	0	1	207	1	207	2.31e-152	420
Msa0127230	MtrunA17_Chr7g0271401	97.585	207	5	1	207	1	207	7.32e-150	414
Msa0127230	MtrunA17_Chr5g0409231	97.101	207	6	1	207	1	207	2.85e-149	412
Msa0127230	MtrunA17_Chr8g0369561	90.196	51	5	134	184	7	57	2.82e-27	98.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0127230	AT3G49010.3	87.435	191	24	0	2	192	1	191	5.83e-126	354
Msa0127230	AT3G49010.6	87.435	191	24	0	2	192	1	191	5.83e-126	354
Msa0127230	AT3G49010.1	87.435	191	24	0	2	192	1	191	5.83e-126	354
Msa0127230	AT3G49010.2	87.435	191	24	0	2	192	1	191	5.83e-126	354
Msa0127230	AT3G49010.7	87.435	191	24	0	2	192	1	191	5.83e-126	354
Msa0127230	AT3G49010.4	86.911	191	23	1	2	192	1	189	1.57e-123	348
Msa0127230	AT5G23900.1	84.946	186	28	0	2	187	1	186	1.19e-118	335
Msa0127230	AT3G48960.1	77.083	192	44	0	2	193	1	192	9.33e-109	310
Msa0127230	AT3G49010.5	62.304	191	19	1	2	192	1	138	3.84e-79	233

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Find 94 sgRNAs with CRISPR-Local

Find 180 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGTCCAGCGGTTGGCCG+GGG	0.418246	1_3:-56485621	None:intergenic
AAATCTTCACTGCCTTCTTT+TGG	0.308723	1_3:-56485596	None:intergenic
AACATTGTTGTGCTTCACCA+TGG	0.595442	1_3:-56485109	None:intergenic
AACGGCGGTACCTGGGAGAG+AGG	0.594400	1_3:-56485001	None:intergenic
AAGAACGGTTCTTGCGGCGA+TGG	0.520566	1_3:-56486677	None:intergenic
AAGAAGCTTGCTCCAACCAT+TGG	0.507390	1_3:+56486642	Msa0127230:CDS
AAGGCGCGCACGCAAGACCA+AGG	0.626116	1_3:+56486764	Msa0127230:CDS
AAGGTCCAGCGGTTGGCCGG+GGG	0.569118	1_3:-56485620	None:intergenic
AAGTGAAGGAATGGCAATTA+GGG	0.375512	1_3:-56484971	None:intergenic
AATGGTTGGAGCAAGCTTCT+TGG	0.375579	1_3:-56486640	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACCAGATCAGTTCAATATTT+AGG	+	chr1_3:56485027-56485046	Msa0127230:intron	30.0%
CCAGATCAGTTCAATATTTA+GGG	+	chr1_3:56485028-56485047	Msa0127230:intron	30.0%
CAAACATGTCAAAAAACATG+AGG	-	chr1_3:56485303-56485322	None:intergenic	30.0%
CAAACAAACAATTTAAGCCA+CGG	-	chr1_3:56485394-56485413	None:intergenic	30.0%
CCGAAAATAGATTAAACTGT+AGG	+	chr1_3:56486047-56486066	Msa0127230:intron	30.0%
TACTCTCAAGTTACTAAACA+TGG	-	chr1_3:56486117-56486136	None:intergenic	30.0%
TGCATATGACACTTAAATGA+TGG	+	chr1_3:56486229-56486248	Msa0127230:intron	30.0%
GCATATGACACTTAAATGAT+GGG	+	chr1_3:56486230-56486249	Msa0127230:intron	30.0%
AATAGCCATATGCAAAAACT+TGG	-	chr1_3:56486449-56486468	None:intergenic	30.0%
TAGTTGAAGACTATAAAGTC+TGG	-	chr1_3:56486548-56486567	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_3	gene	56484855	56487427	56484855	ID=Msa0127230;Name=Msa0127230
chr1_3	mRNA	56484855	56487427	56484855	ID=Msa0127230-mRNA-1;Parent=Msa0127230;Name=Msa0127230-mRNA-1;_AED=0.12;_eAED=0.12;_QI=165\|1\|1\|1\|0.75\|0.6\|5\|287\|207
chr1_3	exon	56484855	56485012	56484855	ID=Msa0127230-mRNA-1:exon:17784;Parent=Msa0127230-mRNA-1
chr1_3	exon	56485104	56485219	56485104	ID=Msa0127230-mRNA-1:exon:17785;Parent=Msa0127230-mRNA-1
chr1_3	exon	56485592	56485725	56485592	ID=Msa0127230-mRNA-1:exon:17786;Parent=Msa0127230-mRNA-1
chr1_3	exon	56486627	56486785	56486627	ID=Msa0127230-mRNA-1:exon:17787;Parent=Msa0127230-mRNA-1
chr1_3	exon	56486919	56487427	56486919	ID=Msa0127230-mRNA-1:exon:17788;Parent=Msa0127230-mRNA-1
chr1_3	five_prime_UTR	56484855	56485012	56484855	ID=Msa0127230-mRNA-1:five_prime_utr;Parent=Msa0127230-mRNA-1
chr1_3	five_prime_UTR	56485104	56485110	56485104	ID=Msa0127230-mRNA-1:five_prime_utr;Parent=Msa0127230-mRNA-1
chr1_3	CDS	56485111	56485219	56485111	ID=Msa0127230-mRNA-1:cds;Parent=Msa0127230-mRNA-1
chr1_3	CDS	56485592	56485725	56485592	ID=Msa0127230-mRNA-1:cds;Parent=Msa0127230-mRNA-1
chr1_3	CDS	56486627	56486785	56486627	ID=Msa0127230-mRNA-1:cds;Parent=Msa0127230-mRNA-1
chr1_3	CDS	56486919	56487140	56486919	ID=Msa0127230-mRNA-1:cds;Parent=Msa0127230-mRNA-1
chr1_3	three_prime_UTR	56487141	56487427	56487141	ID=Msa0127230-mRNA-1:three_prime_utr;Parent=Msa0127230-mRNA-1

Gene Sequence

>Msa0127230

ATGGTGAAGCACAACAATGTTATCCCAAATGAACACTTCCGTAAGCATTGGCAGAACTATGTCAAAACATGGTTCAATCAACCAGCTAGGAAGACCAGGAGACGCTTGGCCCGCCAAAAGAAGGCAGTGAAGATTTTCCCCCGGCCAACCGCTGGACCTTTGAGGCCAATTGTTCACGGTCAAACTGCTAAGTACAACATGAAACTTCGTGCCGGGAAAGGATTTTCTCTTGAAGAGTTGAAGGCTGCCGGTATCCCCAAGAAGCTTGCTCCAACCATTGGCATTTCTGTTGACCATCGCCGCAAGAACCGTTCTTTGGAGGGTCTGCAAGCTAATGTGCAGAGGCTGAAAACATACAAGGCCAAGTTGGTTGTTTTCCCAAGGCGCGCACGCAAGACCAAGGCTGGGGACTCTACACCTGAGGAACTTGCTAATGCCACACAAGTCCAAGGATCATACTTGCCGATTGTGAGGGAGAAGCCAACTGTTGAACTTGTTAAGGTTACAGATGAAATGAAGGCATTCAAAGCTTATTACAAGCTTCGCCTTGAGCGCACCAACAAGCGTCATTTGGGTGCTAGGCTCAAGAGGGCTGCTGAGGCAGAAAAGGAAGACAAGAAGTGA

Protein sequence

>Msa0127230

MVKHNNVIPNEHFRKHWQNYVKTWFNQPARKTRRRLARQKKAVKIFPRPTAGPLRPIVHGQTAKYNMKLRAGKGFSLEELKAAGIPKKLAPTIGISVDHRRKNRSLEGLQANVQRLKTYKAKLVVFPRRARKTKAGDSTPEELANATQVQGSYLPIVREKPTVELVKVTDEMKAFKAYYKLRLERTNKRHLGARLKRAAEAEKEDKK*