AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0438010


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0438010 MtrunA17_Chr7g0263981 98.828 256 3 0 1 256 1 256 0.0 507
Msa0438010 MtrunA17_Chr4g0029331 59.394 165 63 2 27 188 46 209 4.65e-59 188
Msa0438010 MtrunA17_Chr2g0326431 55.705 149 65 1 40 188 41 188 2.24e-51 168
Msa0438010 MtrunA17_Chr2g0325021 44.889 225 114 5 15 232 10 231 9.09e-50 164
Msa0438010 MtrunA17_Chr2g0284411 41.860 215 104 5 40 246 68 269 4.04e-46 155
Msa0438010 MtrunA17_Chr4g0029501 42.211 199 110 4 32 229 65 259 8.29e-46 155
Msa0438010 MtrunA17_Chr4g0029441 42.211 199 110 4 32 229 65 259 4.25e-45 153
Msa0438010 MtrunA17_Chr2g0284451 39.754 244 129 6 26 256 56 294 6.62e-45 152
Msa0438010 MtrunA17_Chr8g0379621 47.929 169 76 5 29 187 76 242 4.96e-44 151
Msa0438010 MtrunA17_Chr2g0326421 40.090 222 128 4 36 255 65 283 5.05e-44 150
Msa0438010 MtrunA17_Chr8g0379631 49.367 158 73 4 35 187 87 242 7.85e-44 150
Msa0438010 MtrunA17_Chr4g0026611 40.969 227 125 5 32 250 69 294 9.03e-44 149
Msa0438010 MtrunA17_Chr2g0284431 40.639 219 109 5 40 250 89 294 1.14e-43 149
Msa0438010 MtrunA17_Chr8g0379611 38.991 218 128 4 29 245 66 279 3.80e-43 149
Msa0438010 MtrunA17_Chr4g0029431 49.032 155 77 2 36 189 63 216 7.54e-40 139
Msa0438010 MtrunA17_Chr2g0326411 45.205 146 78 2 40 184 69 213 5.27e-37 132
Msa0438010 MtrunA17_Chr5g0448001 40.331 181 94 6 39 215 47 217 6.67e-33 120
Msa0438010 MtrunA17_Chr7g0246041 35.632 174 105 4 32 203 176 344 1.58e-23 98.2
Msa0438010 MtrunA17_Chr2g0296571 28.761 226 145 6 35 249 190 410 2.41e-19 86.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0438010 AT5G03170.1 63.776 196 65 3 10 204 7 197 1.03e-81 245
Msa0438010 AT5G60490.1 62.416 149 56 0 40 188 39 187 2.82e-64 201
Msa0438010 AT1G03870.1 45.789 190 101 2 39 228 38 225 3.52e-52 170
Msa0438010 AT2G20520.1 51.366 183 81 4 25 202 21 200 6.73e-52 169
Msa0438010 AT5G44130.1 43.590 195 109 1 39 232 35 229 1.07e-51 169
Msa0438010 AT2G04780.1 38.562 153 88 2 39 189 45 193 4.76e-27 105
Msa0438010 AT2G04780.2 38.562 153 88 2 39 189 45 193 4.76e-27 105
Msa0438010 AT3G60900.1 31.452 248 141 8 32 255 180 422 6.69e-22 94.4
Msa0438010 AT2G45470.1 32.661 248 133 8 32 253 180 419 3.11e-19 86.7
Msa0438010 AT3G46550.1 31.818 176 110 6 30 198 198 370 3.39e-19 86.7
Msa0438010 AT4G12730.1 26.639 244 166 8 18 256 167 402 5.21e-12 65.5

Find 43 sgRNAs with CRISPR-Local

Find 102 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCTTAATGTTACTACTTC+TGG 0.193003 3_3:-2019516 Msa0438010:CDS
AAGACTTTGGTTATGTTTAA+TGG 0.195747 3_3:+2019828 None:intergenic
CTGTTGGTGCAAAGATTGTT+AGG 0.345141 3_3:+2019709 None:intergenic
CAATCAGCTTGCAGTGTATC+AGG 0.355039 3_3:-2019430 Msa0438010:CDS
CCAGAAGTAGTAACATTAAG+AGG 0.370257 3_3:+2019516 None:intergenic
TTGAATGGCTACATAGTTAA+TGG 0.376862 3_3:-2019249 Msa0438010:CDS
ATGTTGTTGTGTCTTGTTTG+TGG 0.393317 3_3:-2019197 Msa0438010:CDS
TTCTCTGGCTTAGTCGGTGC+AGG 0.396164 3_3:+2019342 None:intergenic
TGATCAAGTGCTTCTTCCTA+TGG 0.415143 3_3:-2019406 Msa0438010:CDS
TCGAGTGCTGTTGGTTTGAA+TGG 0.429163 3_3:-2019264 Msa0438010:CDS
AATCAAGTTAATGTAACAAC+TGG 0.448742 3_3:-2019492 Msa0438010:CDS
GAAACTGTAGAAGTTGAAGT+TGG 0.451256 3_3:+2019631 None:intergenic
TGATGCTCCGAAAGGGTCTT+CGG 0.456324 3_3:-2019304 Msa0438010:CDS
GGACTATCCGAAGACCCTTT+CGG 0.481438 3_3:+2019297 None:intergenic
GATGAATGTTGTGAATTGTC+CGG 0.481489 3_3:+2019797 None:intergenic
AACCCTCTACACACACAAGC+TGG 0.488371 3_3:-2019561 Msa0438010:CDS
GCATTCTCAAGCCTAAAATC+AGG 0.498236 3_3:-2019681 Msa0438010:CDS
TCCGACCGCTGCGCCTGCGG+AGG 0.501128 3_3:-2019367 Msa0438010:CDS
CAACTCAACAACTCAAATCA+AGG 0.503271 3_3:-2019732 Msa0438010:CDS
AGGTCCGACCGCTGCGCCTG+CGG 0.504633 3_3:-2019370 Msa0438010:CDS
CGGGCTTCTGATGCTCCGAA+AGG 0.506709 3_3:-2019312 Msa0438010:CDS
GATGGTGCTGGTGATATAGC+TGG 0.507084 3_3:+2019855 None:intergenic
GCTATATCACCAGCACCATC+AGG 0.510604 3_3:-2019852 Msa0438010:CDS
GTCGGTGCAGGAGCCTCCGC+AGG 0.511376 3_3:+2019354 None:intergenic
GTTATGTTTAATGGTCCTGA+TGG 0.521667 3_3:+2019837 None:intergenic
CAAGCTGGAAACAGTGATGA+TGG 0.526045 3_3:-2019546 Msa0438010:CDS
TTCCAGCTTGTGTGTGTAGA+GGG 0.528322 3_3:+2019559 None:intergenic
CTAAGCCAGAGAAAAGTGTC+CGG 0.530199 3_3:-2019332 Msa0438010:CDS
GGGCTTCTGATGCTCCGAAA+GGG 0.534306 3_3:-2019311 Msa0438010:CDS
GCCTCCGCAGGCGCAGCGGT+CGG 0.537525 3_3:+2019366 None:intergenic
CTTTCGGAGCATCAGAAGCC+CGG 0.539752 3_3:+2019313 None:intergenic
GAAAGGGTCTTCGGATAGTC+CGG 0.540038 3_3:-2019295 Msa0438010:CDS
GCTGATGATTCGAGTGCTGT+TGG 0.549087 3_3:-2019273 Msa0438010:CDS
TAAGCCAGAGAAAAGTGTCC+GGG 0.555324 3_3:-2019331 Msa0438010:CDS
TTTCCAGCTTGTGTGTGTAG+AGG 0.556847 3_3:+2019558 None:intergenic
AGGAGCCTCCGCAGGCGCAG+CGG 0.574666 3_3:+2019362 None:intergenic
TTTAATGGTCCTGATGGTGC+TGG 0.578425 3_3:+2019843 None:intergenic
CTTGAGAATGCATTATCTGT+TGG 0.578528 3_3:+2019693 None:intergenic
CAAACAAGACACAACAACAT+TGG 0.585876 3_3:+2019200 None:intergenic
AAATTGAATAGCTTAGCCAT+TGG 0.595840 3_3:+2019942 None:intergenic
TGTGACATTGTGTAAAGTGT+TGG 0.619737 3_3:+2019600 None:intergenic
ACAGCACTCGAATCATCAGC+CGG 0.625967 3_3:+2019276 None:intergenic
GAGTTGCGAATTGATTCGAT+CGG 0.631407 3_3:+2019749 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AATGCATTGAGAAAAAATAG+AGG + chr3_3:2019218-2019237 None:intergenic 25.0%
! AAGACTTTGGTTATGTTTAA+TGG + chr3_3:2019305-2019324 None:intergenic 25.0%
! AATCAAGTTAATGTAACAAC+TGG - chr3_3:2019638-2019657 Msa0438010:CDS 25.0%
! AATGCATTGAGAAAAAATAG+AGG + chr3_3:2019218-2019237 None:intergenic 25.0%
! AAGACTTTGGTTATGTTTAA+TGG + chr3_3:2019305-2019324 None:intergenic 25.0%
! AATCAAGTTAATGTAACAAC+TGG - chr3_3:2019638-2019657 Msa0438010:CDS 25.0%
! AGTTTAGTGTTCCTGATTTT+AGG + chr3_3:2019463-2019482 None:intergenic 30.0%
TTGAATGGCTACATAGTTAA+TGG - chr3_3:2019881-2019900 Msa0438010:CDS 30.0%
! AGTTTAGTGTTCCTGATTTT+AGG + chr3_3:2019463-2019482 None:intergenic 30.0%
TTGAATGGCTACATAGTTAA+TGG - chr3_3:2019881-2019900 Msa0438010:CDS 30.0%
GTTATGTTTAATGGTCCTGA+TGG + chr3_3:2019296-2019315 None:intergenic 35.0%
ACCAAAGTCTTAGAAAAAGC+CGG - chr3_3:2019314-2019333 Msa0438010:CDS 35.0%
GATGAATGTTGTGAATTGTC+CGG + chr3_3:2019336-2019355 None:intergenic 35.0%
CAACTCAACAACTCAAATCA+AGG - chr3_3:2019398-2019417 Msa0438010:CDS 35.0%
CTTGAGAATGCATTATCTGT+TGG + chr3_3:2019440-2019459 None:intergenic 35.0%
GAAACTGTAGAAGTTGAAGT+TGG + chr3_3:2019502-2019521 None:intergenic 35.0%
TGTGACATTGTGTAAAGTGT+TGG + chr3_3:2019533-2019552 None:intergenic 35.0%
CCAGAAGTAGTAACATTAAG+AGG + chr3_3:2019617-2019636 None:intergenic 35.0%
CCTCTTAATGTTACTACTTC+TGG - chr3_3:2019614-2019633 Msa0438010:CDS 35.0%
CAAACAAGACACAACAACAT+TGG + chr3_3:2019933-2019952 None:intergenic 35.0%
!! ATGTTGTTGTGTCTTGTTTG+TGG - chr3_3:2019933-2019952 Msa0438010:CDS 35.0%
GTTATGTTTAATGGTCCTGA+TGG + chr3_3:2019296-2019315 None:intergenic 35.0%
ACCAAAGTCTTAGAAAAAGC+CGG - chr3_3:2019314-2019333 Msa0438010:CDS 35.0%
GATGAATGTTGTGAATTGTC+CGG + chr3_3:2019336-2019355 None:intergenic 35.0%
CAACTCAACAACTCAAATCA+AGG - chr3_3:2019398-2019417 Msa0438010:CDS 35.0%
CTTGAGAATGCATTATCTGT+TGG + chr3_3:2019440-2019459 None:intergenic 35.0%
GAAACTGTAGAAGTTGAAGT+TGG + chr3_3:2019502-2019521 None:intergenic 35.0%
TGTGACATTGTGTAAAGTGT+TGG + chr3_3:2019533-2019552 None:intergenic 35.0%
CCAGAAGTAGTAACATTAAG+AGG + chr3_3:2019617-2019636 None:intergenic 35.0%
CCTCTTAATGTTACTACTTC+TGG - chr3_3:2019614-2019633 Msa0438010:CDS 35.0%
CAAACAAGACACAACAACAT+TGG + chr3_3:2019933-2019952 None:intergenic 35.0%
!! ATGTTGTTGTGTCTTGTTTG+TGG - chr3_3:2019933-2019952 Msa0438010:CDS 35.0%
!!! TCCGGCTTTTTCTAAGACTT+TGG + chr3_3:2019318-2019337 None:intergenic 40.0%
!! GAGTTGCGAATTGATTCGAT+CGG + chr3_3:2019384-2019403 None:intergenic 40.0%
CTGTTGGTGCAAAGATTGTT+AGG + chr3_3:2019424-2019443 None:intergenic 40.0%
GCATTCTCAAGCCTAAAATC+AGG - chr3_3:2019449-2019468 Msa0438010:CDS 40.0%
TGATCAAGTGCTTCTTCCTA+TGG - chr3_3:2019724-2019743 Msa0438010:CDS 40.0%
! CTTCTTCCTATGGCACTTTT+TGG - chr3_3:2019734-2019753 Msa0438010:CDS 40.0%
!! ACACTTTTCTCTGGCTTAGT+CGG + chr3_3:2019797-2019816 None:intergenic 40.0%
!!! TCCGGCTTTTTCTAAGACTT+TGG + chr3_3:2019318-2019337 None:intergenic 40.0%
!! GAGTTGCGAATTGATTCGAT+CGG + chr3_3:2019384-2019403 None:intergenic 40.0%
CTGTTGGTGCAAAGATTGTT+AGG + chr3_3:2019424-2019443 None:intergenic 40.0%
GCATTCTCAAGCCTAAAATC+AGG - chr3_3:2019449-2019468 Msa0438010:CDS 40.0%
TGATCAAGTGCTTCTTCCTA+TGG - chr3_3:2019724-2019743 Msa0438010:CDS 40.0%
! CTTCTTCCTATGGCACTTTT+TGG - chr3_3:2019734-2019753 Msa0438010:CDS 40.0%
!! ACACTTTTCTCTGGCTTAGT+CGG + chr3_3:2019797-2019816 None:intergenic 40.0%
! TTTAATGGTCCTGATGGTGC+TGG + chr3_3:2019290-2019309 None:intergenic 45.0%
TTCCAGCTTGTGTGTGTAGA+GGG + chr3_3:2019574-2019593 None:intergenic 45.0%
TTTCCAGCTTGTGTGTGTAG+AGG + chr3_3:2019575-2019594 None:intergenic 45.0%
CAAGCTGGAAACAGTGATGA+TGG - chr3_3:2019584-2019603 Msa0438010:CDS 45.0%
CAATCAGCTTGCAGTGTATC+AGG - chr3_3:2019700-2019719 Msa0438010:CDS 45.0%
!! CCTTGTCCAAAAAGTGCCAT+AGG + chr3_3:2019743-2019762 None:intergenic 45.0%
!! CCTATGGCACTTTTTGGACA+AGG - chr3_3:2019740-2019759 Msa0438010:CDS 45.0%
!! CTAAGCCAGAGAAAAGTGTC+CGG - chr3_3:2019798-2019817 Msa0438010:CDS 45.0%
!! TAAGCCAGAGAAAAGTGTCC+GGG - chr3_3:2019799-2019818 Msa0438010:CDS 45.0%
!! TCGAGTGCTGTTGGTTTGAA+TGG - chr3_3:2019866-2019885 Msa0438010:CDS 45.0%
! TTTAATGGTCCTGATGGTGC+TGG + chr3_3:2019290-2019309 None:intergenic 45.0%
TTCCAGCTTGTGTGTGTAGA+GGG + chr3_3:2019574-2019593 None:intergenic 45.0%
TTTCCAGCTTGTGTGTGTAG+AGG + chr3_3:2019575-2019594 None:intergenic 45.0%
CAAGCTGGAAACAGTGATGA+TGG - chr3_3:2019584-2019603 Msa0438010:CDS 45.0%
CAATCAGCTTGCAGTGTATC+AGG - chr3_3:2019700-2019719 Msa0438010:CDS 45.0%
!! CCTTGTCCAAAAAGTGCCAT+AGG + chr3_3:2019743-2019762 None:intergenic 45.0%
!! CCTATGGCACTTTTTGGACA+AGG - chr3_3:2019740-2019759 Msa0438010:CDS 45.0%
!! CTAAGCCAGAGAAAAGTGTC+CGG - chr3_3:2019798-2019817 Msa0438010:CDS 45.0%
!! TAAGCCAGAGAAAAGTGTCC+GGG - chr3_3:2019799-2019818 Msa0438010:CDS 45.0%
!! TCGAGTGCTGTTGGTTTGAA+TGG - chr3_3:2019866-2019885 Msa0438010:CDS 45.0%
!! GATGGTGCTGGTGATATAGC+TGG + chr3_3:2019278-2019297 None:intergenic 50.0%
GCTATATCACCAGCACCATC+AGG - chr3_3:2019278-2019297 Msa0438010:CDS 50.0%
AACCCTCTACACACACAAGC+TGG - chr3_3:2019569-2019588 Msa0438010:CDS 50.0%
TGATGCTCCGAAAGGGTCTT+CGG - chr3_3:2019826-2019845 Msa0438010:CDS 50.0%
GGACTATCCGAAGACCCTTT+CGG + chr3_3:2019836-2019855 None:intergenic 50.0%
GAAAGGGTCTTCGGATAGTC+CGG - chr3_3:2019835-2019854 Msa0438010:CDS 50.0%
ACAGCACTCGAATCATCAGC+CGG + chr3_3:2019857-2019876 None:intergenic 50.0%
! GCTGATGATTCGAGTGCTGT+TGG - chr3_3:2019857-2019876 Msa0438010:CDS 50.0%
!! GATGGTGCTGGTGATATAGC+TGG + chr3_3:2019278-2019297 None:intergenic 50.0%
GCTATATCACCAGCACCATC+AGG - chr3_3:2019278-2019297 Msa0438010:CDS 50.0%
AACCCTCTACACACACAAGC+TGG - chr3_3:2019569-2019588 Msa0438010:CDS 50.0%
TGATGCTCCGAAAGGGTCTT+CGG - chr3_3:2019826-2019845 Msa0438010:CDS 50.0%
GGACTATCCGAAGACCCTTT+CGG + chr3_3:2019836-2019855 None:intergenic 50.0%
GAAAGGGTCTTCGGATAGTC+CGG - chr3_3:2019835-2019854 Msa0438010:CDS 50.0%
ACAGCACTCGAATCATCAGC+CGG + chr3_3:2019857-2019876 None:intergenic 50.0%
! GCTGATGATTCGAGTGCTGT+TGG - chr3_3:2019857-2019876 Msa0438010:CDS 50.0%
! TTCTCTGGCTTAGTCGGTGC+AGG + chr3_3:2019791-2019810 None:intergenic 55.0%
! GAAGCCCGGACACTTTTCTC+TGG + chr3_3:2019806-2019825 None:intergenic 55.0%
! CTTTCGGAGCATCAGAAGCC+CGG + chr3_3:2019820-2019839 None:intergenic 55.0%
GGGCTTCTGATGCTCCGAAA+GGG - chr3_3:2019819-2019838 Msa0438010:CDS 55.0%
! TTCTCTGGCTTAGTCGGTGC+AGG + chr3_3:2019791-2019810 None:intergenic 55.0%
! GAAGCCCGGACACTTTTCTC+TGG + chr3_3:2019806-2019825 None:intergenic 55.0%
! CTTTCGGAGCATCAGAAGCC+CGG + chr3_3:2019820-2019839 None:intergenic 55.0%
GGGCTTCTGATGCTCCGAAA+GGG - chr3_3:2019819-2019838 Msa0438010:CDS 55.0%
CGGGCTTCTGATGCTCCGAA+AGG - chr3_3:2019818-2019837 Msa0438010:CDS 60.0%
CGGGCTTCTGATGCTCCGAA+AGG - chr3_3:2019818-2019837 Msa0438010:CDS 60.0%
AGGTCCGACCGCTGCGCCTG+CGG - chr3_3:2019760-2019779 Msa0438010:CDS 75.0%
AGGAGCCTCCGCAGGCGCAG+CGG + chr3_3:2019771-2019790 None:intergenic 75.0%
GTCGGTGCAGGAGCCTCCGC+AGG + chr3_3:2019779-2019798 None:intergenic 75.0%
AGGTCCGACCGCTGCGCCTG+CGG - chr3_3:2019760-2019779 Msa0438010:CDS 75.0%
AGGAGCCTCCGCAGGCGCAG+CGG + chr3_3:2019771-2019790 None:intergenic 75.0%
GTCGGTGCAGGAGCCTCCGC+AGG + chr3_3:2019779-2019798 None:intergenic 75.0%
! TCCGACCGCTGCGCCTGCGG+AGG - chr3_3:2019763-2019782 Msa0438010:CDS 80.0%
! GCCTCCGCAGGCGCAGCGGT+CGG + chr3_3:2019767-2019786 None:intergenic 80.0%
! TCCGACCGCTGCGCCTGCGG+AGG - chr3_3:2019763-2019782 Msa0438010:CDS 80.0%
! GCCTCCGCAGGCGCAGCGGT+CGG + chr3_3:2019767-2019786 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 2019191 2019961 2019191 ID=Msa0438010;Name=Msa0438010
chr3_3 mRNA 2019191 2019961 2019191 ID=Msa0438010-mRNA-1;Parent=Msa0438010;Name=Msa0438010-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|256
chr3_3 exon 2019191 2019961 2019191 ID=Msa0438010-mRNA-1:exon:464;Parent=Msa0438010-mRNA-1
chr3_3 CDS 2019191 2019961 2019191 ID=Msa0438010-mRNA-1:cds;Parent=Msa0438010-mRNA-1
Gene Sequence

>Msa0438010

ATGGCTAAGCTATTCAATTTCTTTCCTCTATTTTTTCTCAATGCATTCATCATTGTGACACTGTTTCAAAGAATTTCAGCTCAACCAGCTATATCACCAGCACCATCAGGACCATTAAACATAACCAAAGTCTTAGAAAAAGCCGGACAATTCACAACATTCATCAAACTACTAAAAGCAACACAAGTATCCGATCGAATCAATTCGCAACTCAACAACTCAAATCAAGGCCTAACAATCTTTGCACCAACAGATAATGCATTCTCAAGCCTAAAATCAGGAACACTAAACTCAATCAGCACACAAAACCAACTTCAACTTCTACAGTTTCATATTCTACCAACACTTTACACAATGTCACAGTTTCAAACTGCAAGTAACCCTCTACACACACAAGCTGGAAACAGTGATGATGGAGAGTATCCTCTTAATGTTACTACTTCTGGAAATCAAGTTAATGTAACAACTGGTGTGATTGATACAACAGTTTCTAATACTATTTACAGTGACAATCAGCTTGCAGTGTATCAGGTTGATCAAGTGCTTCTTCCTATGGCACTTTTTGGACAAGGTCCGACCGCTGCGCCTGCGGAGGCTCCTGCACCGACTAAGCCAGAGAAAAGTGTCCGGGCTTCTGATGCTCCGAAAGGGTCTTCGGATAGTCCGGCTGATGATTCGAGTGCTGTTGGTTTGAATGGCTACATAGTTAATGGTGCTACATTGTTTGTTGCTGTTTTTGCCAATGTTGTTGTGTCTTGTTTGTGGATGTAA

Protein sequence

>Msa0438010

MAKLFNFFPLFFLNAFIIVTLFQRISAQPAISPAPSGPLNITKVLEKAGQFTTFIKLLKATQVSDRINSQLNNSNQGLTIFAPTDNAFSSLKSGTLNSISTQNQLQLLQFHILPTLYTMSQFQTASNPLHTQAGNSDDGEYPLNVTTSGNQVNVTTGVIDTTVSNTIYSDNQLAVYQVDQVLLPMALFGQGPTAAPAEAPAPTKPEKSVRASDAPKGSSDSPADDSSAVGLNGYIVNGATLFVAVFANVVVSCLWM*