AlfalfaGEDB | Msa0440600

M. sativa cultivar zhongmu-4 / Msa0440600

KEGG: -
GO: GO:0002376 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0009314 GO:0009416 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009814 GO:0009816 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044093 GO:0044424 GO:0044444 GO:0044464 GO:0045087 GO:0048518 GO:0050789 GO:0050794 GO:0050896 GO:0051090 GO:0051091 GO:0051171 GO:0051252 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0065009 GO:0071214 GO:0071478 GO:0071482 GO:0080090 GO:0098542 GO:0104004 GO:1903506 GO:2000112 GO:2001141
PFAM: PF05678.17

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0440600	XP_003598460.2	96.875	128	4	0	1	128	8	135	7.23e-86	256

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0440600	I3S4V2	96.875	128	4	0	1	128	8	135	3.45e-86	256

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0433530	Msa0440600	0.812550	4.007922e-51	-8.615850e-47
Msa0440600	Msa0477260	0.808013	3.814883e-50	-8.615850e-47
Msa0440600	Msa0731180	0.817354	3.444882e-52	-8.615850e-47
Msa0440600	Msa0771580	0.811187	7.934608e-51	-8.615850e-47
Msa0440600	Msa0947760	0.809521	1.816433e-50	-8.615850e-47
Msa0440600	Msa1023180	0.814788	1.288870e-51	-8.615850e-47
Msa0440600	Msa1043050	0.810847	9.404553e-51	-8.615850e-47
Msa0440600	Msa1150710	0.811674	6.222583e-51	-8.615850e-47
Msa0440600	Msa1306750	0.802636	5.107121e-49	-8.615850e-47
Msa0440600	Msa1348600	0.804545	2.051756e-49	-8.615850e-47
Msa0440600	Msa1448630	0.819112	1.377826e-52	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0440600	MtrunA17_Chr3g0082031	96.875	128	4	0	1	128	8	135	6.65e-90	256
Msa0440600	MtrunA17_Chr8g0353491	49.194	124	53	3	8	127	2	119	1.94e-29	103

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0440600	AT3G56710.2	55.102	49	22	0	4	52	22	70	7.73e-11	56.6
Msa0440600	AT3G56710.1	55.102	49	22	0	4	52	22	70	7.73e-11	56.6

Find 15 sgRNAs with CRISPR-Local

Find 16 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATTGAAGCTGTCCATTTAA+TGG	0.171403	3_3:-8084903	None:intergenic
TCCTCAGTGGAGCCATTAAA+TGG	0.317195	3_3:+8084891	Msa0440600:CDS
GCACTAGTCTTCACTTTCAT+AGG	0.354513	3_3:-8084654	None:intergenic
TTATGCCTGATTACTCTACT+TGG	0.443981	3_3:+8084838	Msa0440600:CDS
TTCATCCAAGTAGAGTAATC+AGG	0.456712	3_3:-8084843	None:intergenic
CAATGTTGCTGATATGTTTG+TGG	0.462429	3_3:+8084722	Msa0440600:CDS
CACAAACATATCAGCAACAT+TGG	0.506499	3_3:-8084721	None:intergenic
AGTGATAATAATTCTAGTGA+AGG	0.551796	3_3:+8084813	Msa0440600:CDS
TGTTAACAACCATGTGATGA+AGG	0.575242	3_3:+8084575	Msa0440600:CDS
AAACAAATTCAATTCAACAA+TGG	0.630769	3_3:+8084787	Msa0440600:CDS
GCACTTGTACAAGAGCTCAC+AGG	0.635402	3_3:+8084690	Msa0440600:CDS
AAAAGTGAGATAAACATGCT+TGG	0.646394	3_3:+8084552	None:intergenic
GTTAACAACCATGTGATGAA+GGG	0.652734	3_3:+8084576	Msa0440600:CDS
TTACAATTTGAGATCCTCAG+TGG	0.673563	3_3:+8084878	Msa0440600:CDS
TCCATTTAATGGCTCCACTG+AGG	0.684082	3_3:-8084892	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAACAAATTCAATTCAACAA+TGG	+	chr3_3:8084787-8084806	Msa0440600:CDS	20.0%
!	GAACAAAAAGAACAAAAAAG+AGG	+	chr3_3:8084608-8084627	Msa0440600:CDS	25.0%
!	AGTGATAATAATTCTAGTGA+AGG	+	chr3_3:8084813-8084832	Msa0440600:CDS	25.0%
	TATTGAAGCTGTCCATTTAA+TGG	-	chr3_3:8084906-8084925	None:intergenic	30.0%
	TGTTAACAACCATGTGATGA+AGG	+	chr3_3:8084575-8084594	Msa0440600:CDS	35.0%
	GTTAACAACCATGTGATGAA+GGG	+	chr3_3:8084576-8084595	Msa0440600:CDS	35.0%
	CACAAACATATCAGCAACAT+TGG	-	chr3_3:8084724-8084743	None:intergenic	35.0%
	CAATGTTGCTGATATGTTTG+TGG	+	chr3_3:8084722-8084741	Msa0440600:CDS	35.0%
	TTATGCCTGATTACTCTACT+TGG	+	chr3_3:8084838-8084857	Msa0440600:CDS	35.0%
	TTCATCCAAGTAGAGTAATC+AGG	-	chr3_3:8084846-8084865	None:intergenic	35.0%
!	TTACAATTTGAGATCCTCAG+TGG	+	chr3_3:8084878-8084897	Msa0440600:CDS	35.0%
!!!	GCTTTTTTCCCTTCATCACA+TGG	-	chr3_3:8084587-8084606	None:intergenic	40.0%
!!	GCACTAGTCTTCACTTTCAT+AGG	-	chr3_3:8084657-8084676	None:intergenic	40.0%
	TCCTCAGTGGAGCCATTAAA+TGG	+	chr3_3:8084891-8084910	Msa0440600:CDS	45.0%
	TCCATTTAATGGCTCCACTG+AGG	-	chr3_3:8084895-8084914	None:intergenic	45.0%
	GCACTTGTACAAGAGCTCAC+AGG	+	chr3_3:8084690-8084709	Msa0440600:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr3_3	gene	8084567	8084953	8084567	ID=Msa0440600;Name=Msa0440600
chr3_3	mRNA	8084567	8084953	8084567	ID=Msa0440600-mRNA-1;Parent=Msa0440600;Name=Msa0440600-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|128
chr3_3	exon	8084567	8084953	8084567	ID=Msa0440600-mRNA-1:exon:1675;Parent=Msa0440600-mRNA-1
chr3_3	CDS	8084567	8084953	8084567	ID=Msa0440600-mRNA-1:cds;Parent=Msa0440600-mRNA-1

Gene Sequence

>Msa0440600

ATGCTTGGTGTTAACAACCATGTGATGAAGGGAAAAAAGCAGAACAAAAAGAACAAAAAAGAGGTAAAAGTTACATATATTTCAAGTCCTATGAAAGTGAAGACTAGTGCTTCAAATTTTAGAGCACTTGTACAAGAGCTCACAGGTCAAGACTCCAATGTTGCTGATATGTTTGTGGAAGTTAATGATTTTGTTCATGTTGATGATGTTCAAAATCAACAAACAAATTCAATTCAACAATGGAGTAGTGATAATAATTCTAGTGAAGGTTTTATGCCTGATTACTCTACTTGGATGAAACTTGAAGATGATTACAATTTGAGATCCTCAGTGGAGCCATTAAATGGACAGCTTCAATATGATTTTTTGAGTTTTGATATGATATAG

Protein sequence

>Msa0440600

MLGVNNHVMKGKKQNKKNKKEVKVTYISSPMKVKTSASNFRALVQELTGQDSNVADMFVEVNDFVHVDDVQNQQTNSIQQWSSDNNSSEGFMPDYSTWMKLEDDYNLRSSVEPLNGQLQYDFLSFDMI*