AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0947760


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0947760 MtrunA17_Chr6g0462391 80.282 71 10 2 1 71 1 67 1.40e-27 96.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 18 sgRNAs with CRISPR-Local

Find 146 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCAGAACCTTTGTACTTT+AGG 0.208125 6_3:-30988091 Msa0947760:five_prime_UTR
ATATTTGACTATTATCTCTT+TGG 0.278759 6_3:+30988184 None:intergenic
GTTGACACCACTTCTCAAAA+TGG 0.284964 6_3:-30987844 Msa0947760:CDS
TTAATGATTGAGTTTGTTGT+TGG 0.374228 6_3:+30987926 None:intergenic
TTGAGAGAGAGATGCATCTA+TGG 0.377974 6_3:-30988029 Msa0947760:exon
GAATCTCTCAGATTCTTTGT+TGG 0.383111 6_3:+30988006 None:intergenic
ATCATCAAGCAGTTTCTGTT+GGG 0.399002 6_3:+30987900 None:intergenic
CATCATCAAGCAGTTTCTGT+TGG 0.402774 6_3:+30987899 None:intergenic
TAACAGAAGGTCATGACATA+TGG 0.445027 6_3:+30987810 None:intergenic
AGAGCCTTGTTTGAGAGGTA+AGG 0.487732 6_3:+30986666 None:intergenic
ACCTCTCAAACAAGGCTCTA+GGG 0.512728 6_3:-30986662 Msa0947760:CDS
TACCTCTCAAACAAGGCTCT+AGG 0.528088 6_3:-30986663 Msa0947760:CDS
ACCCTAGAGCCTTGTTTGAG+AGG 0.529926 6_3:+30986661 None:intergenic
TACTGTTGTTTCTGCTGTGG+CGG 0.585711 6_3:-30987772 Msa0947760:intron
TGTTACTGTTGTTTCTGCTG+TGG 0.590386 6_3:-30987775 Msa0947760:CDS
AGCAAGAGAGAAGTAACAGA+AGG 0.609065 6_3:+30987797 None:intergenic
TAAATATACCTAAAGTACAA+AGG 0.648678 6_3:+30988083 None:intergenic
AGAACCTTACCTCTCAAACA+AGG 0.656416 6_3:-30986670 Msa0947760:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAAAAAAGAAGTAAAAAA+AGG + chr6_3:30987105-30987124 None:intergenic 10.0%
!!! TTTTGAAAATTATGAAAATT+TGG - chr6_3:30987460-30987479 Msa0947760:intron 10.0%
!!! TGAATTTTGATAATTTAATT+TGG - chr6_3:30987716-30987735 Msa0947760:intron 10.0%
!! TTAAAAAAAGAAGTAAAAAA+AGG + chr6_3:30987105-30987124 None:intergenic 10.0%
!!! TTTTGAAAATTATGAAAATT+TGG - chr6_3:30987460-30987479 Msa0947760:intron 10.0%
!!! TGAATTTTGATAATTTAATT+TGG - chr6_3:30987716-30987735 Msa0947760:intron 10.0%
!! TTGAATAAAACTATATTATG+TGG - chr6_3:30987273-30987292 Msa0947760:intron 15.0%
!! AAAACATTCAATTATGTATA+TGG - chr6_3:30987422-30987441 Msa0947760:intron 15.0%
!!! TGAAATTGATAATGAAATAA+TGG - chr6_3:30987907-30987926 Msa0947760:CDS 15.0%
!! AACTGAAATATAAATAATTG+AGG + chr6_3:30988033-30988052 None:intergenic 15.0%
!! TTGAATAAAACTATATTATG+TGG - chr6_3:30987273-30987292 Msa0947760:intron 15.0%
!! AAAACATTCAATTATGTATA+TGG - chr6_3:30987422-30987441 Msa0947760:intron 15.0%
!!! TGAAATTGATAATGAAATAA+TGG - chr6_3:30987907-30987926 Msa0947760:CDS 15.0%
!! AACTGAAATATAAATAATTG+AGG + chr6_3:30988033-30988052 None:intergenic 15.0%
!! TAAATATACCTAAAGTACAA+AGG + chr6_3:30986754-30986773 None:intergenic 20.0%
!!! ATTGTTTTATTCGTTTTTAC+TGG - chr6_3:30987157-30987176 Msa0947760:intron 20.0%
!!! TTTTCATAATTTTCAAAACG+TGG + chr6_3:30987458-30987477 None:intergenic 20.0%
!!! TTAATTTAGTTGATTTTGGA+TGG + chr6_3:30987535-30987554 None:intergenic 20.0%
!!! ATGGTTAATTTAGTTGATTT+TGG + chr6_3:30987539-30987558 None:intergenic 20.0%
!! TAGTGTAATAGTGTTTATTA+GGG - chr6_3:30987687-30987706 Msa0947760:intron 20.0%
!! ATGATTATGATTAATCAAAC+TGG - chr6_3:30987779-30987798 Msa0947760:CDS 20.0%
!! TAAATATACCTAAAGTACAA+AGG + chr6_3:30986754-30986773 None:intergenic 20.0%
!!! ATTGTTTTATTCGTTTTTAC+TGG - chr6_3:30987157-30987176 Msa0947760:intron 20.0%
!!! TTTTCATAATTTTCAAAACG+TGG + chr6_3:30987458-30987477 None:intergenic 20.0%
!!! TTAATTTAGTTGATTTTGGA+TGG + chr6_3:30987535-30987554 None:intergenic 20.0%
!!! ATGGTTAATTTAGTTGATTT+TGG + chr6_3:30987539-30987558 None:intergenic 20.0%
!! TAGTGTAATAGTGTTTATTA+GGG - chr6_3:30987687-30987706 Msa0947760:intron 20.0%
!! ATGATTATGATTAATCAAAC+TGG - chr6_3:30987779-30987798 Msa0947760:CDS 20.0%
!!! TTAATGATTGAGTTTGTTGT+TGG + chr6_3:30986911-30986930 None:intergenic 25.0%
! CAACCTAACAATAACAATAT+CGG + chr6_3:30987348-30987367 None:intergenic 25.0%
! GTAGTGTAATAGTGTTTATT+AGG - chr6_3:30987686-30987705 Msa0947760:intron 25.0%
!!! TTAATTTGGTGAAGATGTTT+TGG - chr6_3:30987730-30987749 Msa0947760:intron 25.0%
!!! TTGGTTTTGATTTTTTGTCT+TGG - chr6_3:30987749-30987768 Msa0947760:intron 25.0%
! GAAATAATGGTTTCAAATTG+CGG - chr6_3:30987920-30987939 Msa0947760:CDS 25.0%
! TTATATTTCAGTTGCAATTG+CGG - chr6_3:30988040-30988059 Msa0947760:exon 25.0%
!!! TTAATGATTGAGTTTGTTGT+TGG + chr6_3:30986911-30986930 None:intergenic 25.0%
! CAACCTAACAATAACAATAT+CGG + chr6_3:30987348-30987367 None:intergenic 25.0%
! GTAGTGTAATAGTGTTTATT+AGG - chr6_3:30987686-30987705 Msa0947760:intron 25.0%
!!! TTAATTTGGTGAAGATGTTT+TGG - chr6_3:30987730-30987749 Msa0947760:intron 25.0%
!!! TTGGTTTTGATTTTTTGTCT+TGG - chr6_3:30987749-30987768 Msa0947760:intron 25.0%
! GAAATAATGGTTTCAAATTG+CGG - chr6_3:30987920-30987939 Msa0947760:CDS 25.0%
! TTATATTTCAGTTGCAATTG+CGG - chr6_3:30988040-30988059 Msa0947760:exon 25.0%
ATTCAGAACCTTTGTACTTT+AGG - chr6_3:30986743-30986762 Msa0947760:intron 30.0%
ATCATCATACCTGAAAAAAC+TGG - chr6_3:30986857-30986876 Msa0947760:intron 30.0%
!!! TATTCCATTCCAGTTTTTTC+AGG + chr6_3:30986869-30986888 None:intergenic 30.0%
AACAATATCGGCATTCTTTA+GGG + chr6_3:30987336-30987355 None:intergenic 30.0%
TAACAATATCGGCATTCTTT+AGG + chr6_3:30987337-30987356 None:intergenic 30.0%
! ATGCCGATATTGTTATTGTT+AGG - chr6_3:30987342-30987361 Msa0947760:intron 30.0%
! CGATATTGTTATTGTTAGGT+TGG - chr6_3:30987346-30987365 Msa0947760:intron 30.0%
! TAGTTGTGTGTGTTAGTTTA+AGG - chr6_3:30987382-30987401 Msa0947760:intron 30.0%
TCAATTCACACTCAAAATTG+TGG + chr6_3:30987507-30987526 None:intergenic 30.0%
GTTAATCACGTTCAAAAAGA+TGG + chr6_3:30987558-30987577 None:intergenic 30.0%
TCGCAAATGTAAGGAAAAAT+GGG - chr6_3:30987584-30987603 Msa0947760:intron 30.0%
AATAGTGTTTATTAGGGAAG+TGG - chr6_3:30987693-30987712 Msa0947760:intron 30.0%
AACTGGTATTTATAGAAGAG+AGG - chr6_3:30987796-30987815 Msa0947760:CDS 30.0%
CTGAATTGACCATTTATTGT+GGG - chr6_3:30987844-30987863 Msa0947760:CDS 30.0%
TGAATTGACCATTTATTGTG+GGG - chr6_3:30987845-30987864 Msa0947760:CDS 30.0%
AGATATATCCCCACAATAAA+TGG + chr6_3:30987856-30987875 None:intergenic 30.0%
! TGTGGGGATATATCTATTTT+TGG - chr6_3:30987861-30987880 Msa0947760:CDS 30.0%
!!! GGATATATCTATTTTTGGTG+AGG - chr6_3:30987866-30987885 Msa0947760:CDS 30.0%
AATTGAAATTTCGACTACAC+AGG + chr6_3:30987993-30988012 None:intergenic 30.0%
TAGTCGAAATTTCAATTGTG+AGG - chr6_3:30987996-30988015 Msa0947760:CDS 30.0%
!! AGATACTATCGATTTCCTTA+TGG + chr6_3:30988129-30988148 None:intergenic 30.0%
! CGATAGTATCTTTTCTGTTA+TGG - chr6_3:30988138-30988157 Msa0947760:five_prime_UTR 30.0%
ATTCAGAACCTTTGTACTTT+AGG - chr6_3:30986743-30986762 Msa0947760:intron 30.0%
ATCATCATACCTGAAAAAAC+TGG - chr6_3:30986857-30986876 Msa0947760:intron 30.0%
!!! TATTCCATTCCAGTTTTTTC+AGG + chr6_3:30986869-30986888 None:intergenic 30.0%
AACAATATCGGCATTCTTTA+GGG + chr6_3:30987336-30987355 None:intergenic 30.0%
TAACAATATCGGCATTCTTT+AGG + chr6_3:30987337-30987356 None:intergenic 30.0%
! ATGCCGATATTGTTATTGTT+AGG - chr6_3:30987342-30987361 Msa0947760:intron 30.0%
! CGATATTGTTATTGTTAGGT+TGG - chr6_3:30987346-30987365 Msa0947760:intron 30.0%
! TAGTTGTGTGTGTTAGTTTA+AGG - chr6_3:30987382-30987401 Msa0947760:intron 30.0%
TCAATTCACACTCAAAATTG+TGG + chr6_3:30987507-30987526 None:intergenic 30.0%
GTTAATCACGTTCAAAAAGA+TGG + chr6_3:30987558-30987577 None:intergenic 30.0%
TCGCAAATGTAAGGAAAAAT+GGG - chr6_3:30987584-30987603 Msa0947760:intron 30.0%
AATAGTGTTTATTAGGGAAG+TGG - chr6_3:30987693-30987712 Msa0947760:intron 30.0%
AACTGGTATTTATAGAAGAG+AGG - chr6_3:30987796-30987815 Msa0947760:CDS 30.0%
CTGAATTGACCATTTATTGT+GGG - chr6_3:30987844-30987863 Msa0947760:CDS 30.0%
TGAATTGACCATTTATTGTG+GGG - chr6_3:30987845-30987864 Msa0947760:CDS 30.0%
AGATATATCCCCACAATAAA+TGG + chr6_3:30987856-30987875 None:intergenic 30.0%
! TGTGGGGATATATCTATTTT+TGG - chr6_3:30987861-30987880 Msa0947760:CDS 30.0%
!!! GGATATATCTATTTTTGGTG+AGG - chr6_3:30987866-30987885 Msa0947760:CDS 30.0%
AATTGAAATTTCGACTACAC+AGG + chr6_3:30987993-30988012 None:intergenic 30.0%
TAGTCGAAATTTCAATTGTG+AGG - chr6_3:30987996-30988015 Msa0947760:CDS 30.0%
!! AGATACTATCGATTTCCTTA+TGG + chr6_3:30988129-30988148 None:intergenic 30.0%
! CGATAGTATCTTTTCTGTTA+TGG - chr6_3:30988138-30988157 Msa0947760:five_prime_UTR 30.0%
GAATCTCTCAGATTCTTTGT+TGG + chr6_3:30986831-30986850 None:intergenic 35.0%
CATACCTGAAAAAACTGGAA+TGG - chr6_3:30986862-30986881 Msa0947760:intron 35.0%
ATCATCAAGCAGTTTCTGTT+GGG + chr6_3:30986937-30986956 None:intergenic 35.0%
!! TACAGTTCCATTTTGAGAAG+TGG + chr6_3:30987000-30987019 None:intergenic 35.0%
TAACAGAAGGTCATGACATA+TGG + chr6_3:30987027-30987046 None:intergenic 35.0%
! CTTTTTGAACGTGATTAACC+AGG - chr6_3:30987559-30987578 Msa0947760:intron 35.0%
GTCGCAAATGTAAGGAAAAA+TGG - chr6_3:30987583-30987602 Msa0947760:intron 35.0%
GCTGAATTGACCATTTATTG+TGG - chr6_3:30987843-30987862 Msa0947760:CDS 35.0%
GAACAACTATACCAGAAACT+TGG + chr6_3:30988103-30988122 None:intergenic 35.0%
GTATAGTTGTTCTTGCCATA+AGG - chr6_3:30988111-30988130 Msa0947760:five_prime_UTR 35.0%
GAATCTCTCAGATTCTTTGT+TGG + chr6_3:30986831-30986850 None:intergenic 35.0%
CATACCTGAAAAAACTGGAA+TGG - chr6_3:30986862-30986881 Msa0947760:intron 35.0%
ATCATCAAGCAGTTTCTGTT+GGG + chr6_3:30986937-30986956 None:intergenic 35.0%
!! TACAGTTCCATTTTGAGAAG+TGG + chr6_3:30987000-30987019 None:intergenic 35.0%
TAACAGAAGGTCATGACATA+TGG + chr6_3:30987027-30987046 None:intergenic 35.0%
! CTTTTTGAACGTGATTAACC+AGG - chr6_3:30987559-30987578 Msa0947760:intron 35.0%
GTCGCAAATGTAAGGAAAAA+TGG - chr6_3:30987583-30987602 Msa0947760:intron 35.0%
GCTGAATTGACCATTTATTG+TGG - chr6_3:30987843-30987862 Msa0947760:CDS 35.0%
GAACAACTATACCAGAAACT+TGG + chr6_3:30988103-30988122 None:intergenic 35.0%
GTATAGTTGTTCTTGCCATA+AGG - chr6_3:30988111-30988130 Msa0947760:five_prime_UTR 35.0%
TTGAGAGAGAGATGCATCTA+TGG - chr6_3:30986805-30986824 Msa0947760:intron 40.0%
CATCATCAAGCAGTTTCTGT+TGG + chr6_3:30986938-30986957 None:intergenic 40.0%
GTTGACACCACTTCTCAAAA+TGG - chr6_3:30986990-30987009 Msa0947760:intron 40.0%
AGCAAGAGAGAAGTAACAGA+AGG + chr6_3:30987040-30987059 None:intergenic 40.0%
!! TGTTACTGTTGTTTCTGCTG+TGG - chr6_3:30987059-30987078 Msa0947760:intron 40.0%
CCACATTTCCTGCATTATGA+AGG + chr6_3:30987970-30987989 None:intergenic 40.0%
CCTTCATAATGCAGGAAATG+TGG - chr6_3:30987967-30987986 Msa0947760:CDS 40.0%
!! ACAGAAGTGTTCCAAGTTTC+TGG - chr6_3:30988089-30988108 Msa0947760:five_prime_UTR 40.0%
AGAACCTTACCTCTCAAACA+AGG - chr6_3:30988164-30988183 Msa0947760:five_prime_UTR 40.0%
TTGAGAGAGAGATGCATCTA+TGG - chr6_3:30986805-30986824 Msa0947760:intron 40.0%
CATCATCAAGCAGTTTCTGT+TGG + chr6_3:30986938-30986957 None:intergenic 40.0%
GTTGACACCACTTCTCAAAA+TGG - chr6_3:30986990-30987009 Msa0947760:intron 40.0%
AGCAAGAGAGAAGTAACAGA+AGG + chr6_3:30987040-30987059 None:intergenic 40.0%
!! TGTTACTGTTGTTTCTGCTG+TGG - chr6_3:30987059-30987078 Msa0947760:intron 40.0%
CCACATTTCCTGCATTATGA+AGG + chr6_3:30987970-30987989 None:intergenic 40.0%
CCTTCATAATGCAGGAAATG+TGG - chr6_3:30987967-30987986 Msa0947760:CDS 40.0%
!! ACAGAAGTGTTCCAAGTTTC+TGG - chr6_3:30988089-30988108 Msa0947760:five_prime_UTR 40.0%
AGAACCTTACCTCTCAAACA+AGG - chr6_3:30988164-30988183 Msa0947760:five_prime_UTR 40.0%
!! TACTGTTGTTTCTGCTGTGG+CGG - chr6_3:30987062-30987081 Msa0947760:intron 45.0%
! AACCAGGTGTCGCAAATGTA+AGG - chr6_3:30987575-30987594 Msa0947760:intron 45.0%
TTCCTTACATTTGCGACACC+TGG + chr6_3:30987580-30987599 None:intergenic 45.0%
CTTGCGAACCTTCATAATGC+AGG - chr6_3:30987959-30987978 Msa0947760:CDS 45.0%
TTCAGTTGCAATTGCGGTGT+AGG - chr6_3:30988046-30988065 Msa0947760:five_prime_UTR 45.0%
!! AGAGCCTTGTTTGAGAGGTA+AGG + chr6_3:30988171-30988190 None:intergenic 45.0%
TACCTCTCAAACAAGGCTCT+AGG - chr6_3:30988171-30988190 Msa0947760:five_prime_UTR 45.0%
ACCTCTCAAACAAGGCTCTA+GGG - chr6_3:30988172-30988191 Msa0947760:five_prime_UTR 45.0%
!! TACTGTTGTTTCTGCTGTGG+CGG - chr6_3:30987062-30987081 Msa0947760:intron 45.0%
! AACCAGGTGTCGCAAATGTA+AGG - chr6_3:30987575-30987594 Msa0947760:intron 45.0%
TTCCTTACATTTGCGACACC+TGG + chr6_3:30987580-30987599 None:intergenic 45.0%
CTTGCGAACCTTCATAATGC+AGG - chr6_3:30987959-30987978 Msa0947760:CDS 45.0%
TTCAGTTGCAATTGCGGTGT+AGG - chr6_3:30988046-30988065 Msa0947760:five_prime_UTR 45.0%
!! AGAGCCTTGTTTGAGAGGTA+AGG + chr6_3:30988171-30988190 None:intergenic 45.0%
TACCTCTCAAACAAGGCTCT+AGG - chr6_3:30988171-30988190 Msa0947760:five_prime_UTR 45.0%
ACCTCTCAAACAAGGCTCTA+GGG - chr6_3:30988172-30988191 Msa0947760:five_prime_UTR 45.0%
GAAGGTTCGCAAGTTAACCG+CGG + chr6_3:30987952-30987971 None:intergenic 50.0%
!! ACCCTAGAGCCTTGTTTGAG+AGG + chr6_3:30988176-30988195 None:intergenic 50.0%
GAAGGTTCGCAAGTTAACCG+CGG + chr6_3:30987952-30987971 None:intergenic 50.0%
!! ACCCTAGAGCCTTGTTTGAG+AGG + chr6_3:30988176-30988195 None:intergenic 50.0%
TCAAATTGCGGTTGCAGCCG+CGG - chr6_3:30987932-30987951 Msa0947760:CDS 55.0%
TCAAATTGCGGTTGCAGCCG+CGG - chr6_3:30987932-30987951 Msa0947760:CDS 55.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 30986653 30988203 30986653 ID=Msa0947760;Name=Msa0947760
chr6_3 mRNA 30986653 30988203 30986653 ID=Msa0947760-mRNA-1;Parent=Msa0947760;Name=Msa0947760-mRNA-1;_AED=0.27;_eAED=0.27;_QI=163|0|0.5|0.5|0|0|2|0|101
chr6_3 exon 30986653 30986690 30986653 ID=Msa0947760-mRNA-1:exon:8191;Parent=Msa0947760-mRNA-1
chr6_3 exon 30987773 30988203 30987773 ID=Msa0947760-mRNA-1:exon:8190;Parent=Msa0947760-mRNA-1
chr6_3 five_prime_UTR 30988041 30988203 30988041 ID=Msa0947760-mRNA-1:five_prime_utr;Parent=Msa0947760-mRNA-1
chr6_3 CDS 30987773 30988040 30987773 ID=Msa0947760-mRNA-1:cds;Parent=Msa0947760-mRNA-1
chr6_3 CDS 30986653 30986690 30986653 ID=Msa0947760-mRNA-1:cds;Parent=Msa0947760-mRNA-1
Gene Sequence

>Msa0947760

ATGCATCTATGGCCAACAAAGAATCTGAGAGATTCATTCAAATCATCATACCTGAAAAAACTGGAATGGAATACTCAAAGAATGAAAAACAACCAACAACAAACTCAATCATTAACAACCCAACAGAAACTGCTTGATGATGAAGACAACAACAACAACAACAATAACAACGTTGTTGACACCACTTCTCAAAATGGAACTGTAACTTCCATATGTCATGACCTTCTGTTACTTCTCTCTTGCTGTTACTGTTGTTTCTGCTGTGGCGAACCTTACCTCTCAAACAAGGCTCTAGGGTCTGTTTGA

Protein sequence

>Msa0947760

MHLWPTKNLRDSFKSSYLKKLEWNTQRMKNNQQQTQSLTTQQKLLDDEDNNNNNNNNVVDTTSQNGTVTSICHDLLLLLSCCYCCFCCGEPYLSNKALGSV*