AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1448630


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1448630 MtrunA17_Chr7g0240411 92.405 79 6 0 1 79 1 79 5.24e-49 149
Msa1448630 MtrunA17_Chr4g0008691 62.963 81 28 2 1 79 1 81 2.30e-32 107
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 38 sgRNAs with CRISPR-Local

Find 117 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTACCTTGGTTTGAAATTA+AGG 0.129246 tig0032236:+36993 None:intergenic
GAGTTCGATATTATGTTAAA+TGG 0.198013 tig0032236:+36184 None:intergenic
CACTTTGATTTACAAAATTA+TGG 0.210495 tig0032236:-36280 Msa1448630:three_prime_UTR
TTCTCTAAACTTTGATATAT+AGG 0.300426 tig0032236:+36700 None:intergenic
ATGAGAACATGAGGCTTGTT+TGG 0.314452 tig0032236:+37081 None:intergenic
ATGAATTGTTACACAATTCA+TGG 0.321299 tig0032236:-36250 Msa1448630:three_prime_UTR
CTAAAGAGTTTAGAAGGATA+AGG 0.353995 tig0032236:-36414 Msa1448630:three_prime_UTR
CTTGAACCTCTCTAACTTTG+AGG 0.354710 tig0032236:-37162 Msa1448630:five_prime_UTR
AGTTGACTAAAGAGTTTAGA+AGG 0.373568 tig0032236:-36420 Msa1448630:three_prime_UTR
GAGTTGGGAGATATATAAAA+AGG 0.385399 tig0032236:+37202 None:intergenic
TTTGTGTCTGCTAGTGCTAA+TGG 0.389664 tig0032236:-36648 Msa1448630:three_prime_UTR
GAGGTACTTGCACTAGTACT+TGG 0.403825 tig0032236:-36808 Msa1448630:CDS
CTTTAGTCAACTTTAGTTGA+AGG 0.419390 tig0032236:+36431 None:intergenic
TAAAGAGTTTAGAAGGATAA+GGG 0.425247 tig0032236:-36413 Msa1448630:three_prime_UTR
ATGAATTGTGTAACAATTCA+TGG 0.427262 tig0032236:+36252 None:intergenic
AAGGATAAGGGATGGATATC+CGG 0.440238 tig0032236:-36401 Msa1448630:three_prime_UTR
TTTGGTTTGAAACAAGTCAA+TGG 0.446404 tig0032236:-36581 Msa1448630:three_prime_UTR
TGCAAATGTTGTGATGGAAT+TGG 0.452852 tig0032236:-36830 Msa1448630:CDS
TTGGTTTGAAACAAGTCAAT+GGG 0.459688 tig0032236:-36580 Msa1448630:three_prime_UTR
TTAGAGAGGTTCAAGAGCTA+TGG 0.483018 tig0032236:+37170 None:intergenic
TTGTGTCTGCTAGTGCTAAT+GGG 0.495526 tig0032236:-36647 Msa1448630:three_prime_UTR
GCTATGGAGCACTGATGAGT+TGG 0.497002 tig0032236:+37186 None:intergenic
CTATGGAGCACTGATGAGTT+GGG 0.500776 tig0032236:+37187 None:intergenic
GTCCTTGCTTCTATAAGTGA+TGG 0.503145 tig0032236:+37021 None:intergenic
AGGATAGGAAACTTGTAGGA+TGG 0.515633 tig0032236:+37222 None:intergenic
GGATAGGAAACTTGTAGGAT+GGG 0.533450 tig0032236:+37223 None:intergenic
GTTGTTTGCAAATGTTGTGA+TGG 0.534057 tig0032236:-36836 Msa1448630:CDS
AATAACTAGTGTGATTTCCA+AGG 0.557652 tig0032236:+36755 None:intergenic
GAGTTTAGAAGGATAAGGGA+TGG 0.574430 tig0032236:-36409 Msa1448630:three_prime_UTR
CTTTCCTTAATTTCAAACCA+AGG 0.576150 tig0032236:-36997 Msa1448630:intron
ATAGGAAACTTGTAGGATGG+GGG 0.578714 tig0032236:+37225 None:intergenic
AACCATCACTTATAGAAGCA+AGG 0.587548 tig0032236:-37023 Msa1448630:CDS
ATAACTTAAAGTGTTCACCT+TGG 0.595348 tig0032236:-36772 Msa1448630:CDS
ATGAAACCTCAAAGTTAGAG+AGG 0.596758 tig0032236:+37156 None:intergenic
AAATGTTGTGATGGAATTGG+AGG 0.597103 tig0032236:-36827 Msa1448630:CDS
ATTAGTAATATGAGAACATG+AGG 0.602881 tig0032236:+37072 None:intergenic
GATAGGAAACTTGTAGGATG+GGG 0.605112 tig0032236:+37224 None:intergenic
AACAAGTTGCAATAGACATG+TGG 0.663724 tig0032236:+36531 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATTATAATTAATTACCT+TGG + tig0032236:36453-36472 None:intergenic 10.0%
!! ATTATTATAATTAATTACCT+TGG + tig0032236:36453-36472 None:intergenic 10.0%
!! AAATAAAAAATTAGATCTAG+TGG + tig0032236:36517-36536 None:intergenic 15.0%
!!! TTATTTTGTTATGTATTTTC+AGG - tig0032236:36543-36562 Msa1448630:three_prime_UTR 15.0%
!!! TATTTTGTTATGTATTTTCA+GGG - tig0032236:36544-36563 Msa1448630:three_prime_UTR 15.0%
!!! TGTGTAATTTTATTTTGTTT+TGG - tig0032236:37074-37093 Msa1448630:CDS 15.0%
!!! GTGTAATTTTATTTTGTTTT+GGG - tig0032236:37075-37094 Msa1448630:CDS 15.0%
!! AAATAAAAAATTAGATCTAG+TGG + tig0032236:36517-36536 None:intergenic 15.0%
!!! TTATTTTGTTATGTATTTTC+AGG - tig0032236:36543-36562 Msa1448630:three_prime_UTR 15.0%
!!! TATTTTGTTATGTATTTTCA+GGG - tig0032236:36544-36563 Msa1448630:three_prime_UTR 15.0%
!!! TGTGTAATTTTATTTTGTTT+TGG - tig0032236:37074-37093 Msa1448630:CDS 15.0%
!!! GTGTAATTTTATTTTGTTTT+GGG - tig0032236:37075-37094 Msa1448630:CDS 15.0%
!! ATATAAAAACATCAATCATG+TGG - tig0032236:36160-36179 Msa1448630:three_prime_UTR 20.0%
!!! AGTTATTTTTGTGATTTTTC+TGG - tig0032236:36691-36710 Msa1448630:three_prime_UTR 20.0%
!! TTCTCTAAACTTTGATATAT+AGG + tig0032236:36733-36752 None:intergenic 20.0%
!!! ATGATTTTTCATTGATGATA+AGG - tig0032236:36965-36984 Msa1448630:intron 20.0%
!!! TGATTTTTCATTGATGATAA+GGG - tig0032236:36966-36985 Msa1448630:intron 20.0%
!!! CACTTTGATTTACAAAATTA+TGG - tig0032236:37150-37169 Msa1448630:five_prime_UTR 20.0%
!!! TATGAAGCATTTTTAACTTT+TGG - tig0032236:37209-37228 Msa1448630:five_prime_UTR 20.0%
!!! ATGAAGCATTTTTAACTTTT+GGG - tig0032236:37210-37229 Msa1448630:five_prime_UTR 20.0%
!! ATATAAAAACATCAATCATG+TGG - tig0032236:36160-36179 Msa1448630:three_prime_UTR 20.0%
!!! AGTTATTTTTGTGATTTTTC+TGG - tig0032236:36691-36710 Msa1448630:three_prime_UTR 20.0%
!! TTCTCTAAACTTTGATATAT+AGG + tig0032236:36733-36752 None:intergenic 20.0%
!!! ATGATTTTTCATTGATGATA+AGG - tig0032236:36965-36984 Msa1448630:intron 20.0%
!!! TGATTTTTCATTGATGATAA+GGG - tig0032236:36966-36985 Msa1448630:intron 20.0%
!!! CACTTTGATTTACAAAATTA+TGG - tig0032236:37150-37169 Msa1448630:five_prime_UTR 20.0%
!!! TATGAAGCATTTTTAACTTT+TGG - tig0032236:37209-37228 Msa1448630:five_prime_UTR 20.0%
!!! ATGAAGCATTTTTAACTTTT+GGG - tig0032236:37210-37229 Msa1448630:five_prime_UTR 20.0%
! ATTAGTAATATGAGAACATG+AGG + tig0032236:36361-36380 None:intergenic 25.0%
!! ATTACCTTGGTTTGAAATTA+AGG + tig0032236:36440-36459 None:intergenic 25.0%
!!! TATATGTGTCCATAGTTTTT+TGG - tig0032236:36831-36850 Msa1448630:CDS 25.0%
! TGTTTCAAACCAAAAAACTA+TGG + tig0032236:36843-36862 None:intergenic 25.0%
! TAAAGAGTTTAGAAGGATAA+GGG - tig0032236:37017-37036 Msa1448630:CDS 25.0%
! ATGAATTGTGTAACAATTCA+TGG + tig0032236:37181-37200 None:intergenic 25.0%
! ATGAATTGTTACACAATTCA+TGG - tig0032236:37180-37199 Msa1448630:five_prime_UTR 25.0%
! GAGTTCGATATTATGTTAAA+TGG + tig0032236:37249-37268 None:intergenic 25.0%
! ATTAGTAATATGAGAACATG+AGG + tig0032236:36361-36380 None:intergenic 25.0%
!! ATTACCTTGGTTTGAAATTA+AGG + tig0032236:36440-36459 None:intergenic 25.0%
!!! TATATGTGTCCATAGTTTTT+TGG - tig0032236:36831-36850 Msa1448630:CDS 25.0%
! TGTTTCAAACCAAAAAACTA+TGG + tig0032236:36843-36862 None:intergenic 25.0%
! TAAAGAGTTTAGAAGGATAA+GGG - tig0032236:37017-37036 Msa1448630:CDS 25.0%
! ATGAATTGTGTAACAATTCA+TGG + tig0032236:37181-37200 None:intergenic 25.0%
! ATGAATTGTTACACAATTCA+TGG - tig0032236:37180-37199 Msa1448630:five_prime_UTR 25.0%
! GAGTTCGATATTATGTTAAA+TGG + tig0032236:37249-37268 None:intergenic 25.0%
AAAAAGGATAGGAAACTTGT+AGG + tig0032236:36215-36234 None:intergenic 30.0%
GGGAGATATATAAAAAGGAT+AGG + tig0032236:36226-36245 None:intergenic 30.0%
GAGTTGGGAGATATATAAAA+AGG + tig0032236:36231-36250 None:intergenic 30.0%
CTTTCCTTAATTTCAAACCA+AGG - tig0032236:36433-36452 Msa1448630:three_prime_UTR 30.0%
! TCAAAGATTTTTGCAACACT+TGG - tig0032236:36570-36589 Msa1448630:three_prime_UTR 30.0%
! ATAACTTAAAGTGTTCACCT+TGG - tig0032236:36658-36677 Msa1448630:three_prime_UTR 30.0%
AATAACTAGTGTGATTTCCA+AGG + tig0032236:36678-36697 None:intergenic 30.0%
!! TTTGGTTTGAAACAAGTCAA+TGG - tig0032236:36849-36868 Msa1448630:CDS 30.0%
! TTGGTTTGAAACAAGTCAAT+GGG - tig0032236:36850-36869 Msa1448630:CDS 30.0%
CTTTAGTCAACTTTAGTTGA+AGG + tig0032236:37002-37021 None:intergenic 30.0%
AGTTGACTAAAGAGTTTAGA+AGG - tig0032236:37010-37029 Msa1448630:CDS 30.0%
CTAAAGAGTTTAGAAGGATA+AGG - tig0032236:37016-37035 Msa1448630:CDS 30.0%
AAAAAGGATAGGAAACTTGT+AGG + tig0032236:36215-36234 None:intergenic 30.0%
GGGAGATATATAAAAAGGAT+AGG + tig0032236:36226-36245 None:intergenic 30.0%
GAGTTGGGAGATATATAAAA+AGG + tig0032236:36231-36250 None:intergenic 30.0%
CTTTCCTTAATTTCAAACCA+AGG - tig0032236:36433-36452 Msa1448630:three_prime_UTR 30.0%
! TCAAAGATTTTTGCAACACT+TGG - tig0032236:36570-36589 Msa1448630:three_prime_UTR 30.0%
! ATAACTTAAAGTGTTCACCT+TGG - tig0032236:36658-36677 Msa1448630:three_prime_UTR 30.0%
AATAACTAGTGTGATTTCCA+AGG + tig0032236:36678-36697 None:intergenic 30.0%
!! TTTGGTTTGAAACAAGTCAA+TGG - tig0032236:36849-36868 Msa1448630:CDS 30.0%
! TTGGTTTGAAACAAGTCAAT+GGG - tig0032236:36850-36869 Msa1448630:CDS 30.0%
CTTTAGTCAACTTTAGTTGA+AGG + tig0032236:37002-37021 None:intergenic 30.0%
AGTTGACTAAAGAGTTTAGA+AGG - tig0032236:37010-37029 Msa1448630:CDS 30.0%
CTAAAGAGTTTAGAAGGATA+AGG - tig0032236:37016-37035 Msa1448630:CDS 30.0%
ATGAAACCTCAAAGTTAGAG+AGG + tig0032236:36277-36296 None:intergenic 35.0%
AACCATCACTTATAGAAGCA+AGG - tig0032236:36407-36426 Msa1448630:three_prime_UTR 35.0%
AATTAGATCTAGTGGAAGAG+AGG + tig0032236:36509-36528 None:intergenic 35.0%
GTTGTTTGCAAATGTTGTGA+TGG - tig0032236:36594-36613 Msa1448630:three_prime_UTR 35.0%
! TGCAAATGTTGTGATGGAAT+TGG - tig0032236:36600-36619 Msa1448630:three_prime_UTR 35.0%
! AAATGTTGTGATGGAATTGG+AGG - tig0032236:36603-36622 Msa1448630:three_prime_UTR 35.0%
AACAAGTTGCAATAGACATG+TGG + tig0032236:36902-36921 None:intergenic 35.0%
! AGCATCTTTTGAGCTAAAAC+CGG + tig0032236:37051-37070 None:intergenic 35.0%
ATGAAACCTCAAAGTTAGAG+AGG + tig0032236:36277-36296 None:intergenic 35.0%
AACCATCACTTATAGAAGCA+AGG - tig0032236:36407-36426 Msa1448630:three_prime_UTR 35.0%
AATTAGATCTAGTGGAAGAG+AGG + tig0032236:36509-36528 None:intergenic 35.0%
GTTGTTTGCAAATGTTGTGA+TGG - tig0032236:36594-36613 Msa1448630:three_prime_UTR 35.0%
! TGCAAATGTTGTGATGGAAT+TGG - tig0032236:36600-36619 Msa1448630:three_prime_UTR 35.0%
! AAATGTTGTGATGGAATTGG+AGG - tig0032236:36603-36622 Msa1448630:three_prime_UTR 35.0%
AACAAGTTGCAATAGACATG+TGG + tig0032236:36902-36921 None:intergenic 35.0%
! AGCATCTTTTGAGCTAAAAC+CGG + tig0032236:37051-37070 None:intergenic 35.0%
ATAGGAAACTTGTAGGATGG+GGG + tig0032236:36208-36227 None:intergenic 40.0%
GATAGGAAACTTGTAGGATG+GGG + tig0032236:36209-36228 None:intergenic 40.0%
GGATAGGAAACTTGTAGGAT+GGG + tig0032236:36210-36229 None:intergenic 40.0%
AGGATAGGAAACTTGTAGGA+TGG + tig0032236:36211-36230 None:intergenic 40.0%
TTAGAGAGGTTCAAGAGCTA+TGG + tig0032236:36263-36282 None:intergenic 40.0%
CTTGAACCTCTCTAACTTTG+AGG - tig0032236:36268-36287 Msa1448630:three_prime_UTR 40.0%
! ATGAGAACATGAGGCTTGTT+TGG + tig0032236:36352-36371 None:intergenic 40.0%
GTCCTTGCTTCTATAAGTGA+TGG + tig0032236:36412-36431 None:intergenic 40.0%
TTTGTGTCTGCTAGTGCTAA+TGG - tig0032236:36782-36801 Msa1448630:CDS 40.0%
TTGTGTCTGCTAGTGCTAAT+GGG - tig0032236:36783-36802 Msa1448630:CDS 40.0%
GAGTTTAGAAGGATAAGGGA+TGG - tig0032236:37021-37040 Msa1448630:CDS 40.0%
AAGGATAAGGGATGGATATC+CGG - tig0032236:37029-37048 Msa1448630:CDS 40.0%
ATAGGAAACTTGTAGGATGG+GGG + tig0032236:36208-36227 None:intergenic 40.0%
GATAGGAAACTTGTAGGATG+GGG + tig0032236:36209-36228 None:intergenic 40.0%
GGATAGGAAACTTGTAGGAT+GGG + tig0032236:36210-36229 None:intergenic 40.0%
AGGATAGGAAACTTGTAGGA+TGG + tig0032236:36211-36230 None:intergenic 40.0%
TTAGAGAGGTTCAAGAGCTA+TGG + tig0032236:36263-36282 None:intergenic 40.0%
CTTGAACCTCTCTAACTTTG+AGG - tig0032236:36268-36287 Msa1448630:three_prime_UTR 40.0%
! ATGAGAACATGAGGCTTGTT+TGG + tig0032236:36352-36371 None:intergenic 40.0%
GTCCTTGCTTCTATAAGTGA+TGG + tig0032236:36412-36431 None:intergenic 40.0%
TTTGTGTCTGCTAGTGCTAA+TGG - tig0032236:36782-36801 Msa1448630:CDS 40.0%
TTGTGTCTGCTAGTGCTAAT+GGG - tig0032236:36783-36802 Msa1448630:CDS 40.0%
GAGTTTAGAAGGATAAGGGA+TGG - tig0032236:37021-37040 Msa1448630:CDS 40.0%
AAGGATAAGGGATGGATATC+CGG - tig0032236:37029-37048 Msa1448630:CDS 40.0%
!! CTATGGAGCACTGATGAGTT+GGG + tig0032236:36246-36265 None:intergenic 45.0%
! GAGGTACTTGCACTAGTACT+TGG - tig0032236:36622-36641 Msa1448630:three_prime_UTR 45.0%
!! CTATGGAGCACTGATGAGTT+GGG + tig0032236:36246-36265 None:intergenic 45.0%
! GAGGTACTTGCACTAGTACT+TGG - tig0032236:36622-36641 Msa1448630:three_prime_UTR 45.0%
!!! ATGTTTTTATATTATTAATT+AGG + tig0032236:36152-36171 None:intergenic 5.0%
!! GCTATGGAGCACTGATGAGT+TGG + tig0032236:36247-36266 None:intergenic 50.0%
!! GCTATGGAGCACTGATGAGT+TGG + tig0032236:36247-36266 None:intergenic 50.0%
GTAGGATGGGGGATGCTGCT+TGG + tig0032236:36197-36216 None:intergenic 60.0%
GTAGGATGGGGGATGCTGCT+TGG + tig0032236:36197-36216 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
tig0032236 gene 36152 37300 36152 ID=Msa1448630;Name=Msa1448630
tig0032236 mRNA 36152 37300 36152 ID=Msa1448630-mRNA-1;Parent=Msa1448630;Name=Msa1448630-mRNA-1;_AED=0.04;_eAED=0.04;_QI=191|1|1|1|1|1|2|608|79
tig0032236 exon 36152 36887 36152 ID=Msa1448630-mRNA-1:exon:16488;Parent=Msa1448630-mRNA-1
tig0032236 exon 36998 37300 36998 ID=Msa1448630-mRNA-1:exon:16487;Parent=Msa1448630-mRNA-1
tig0032236 five_prime_UTR 37110 37300 37110 ID=Msa1448630-mRNA-1:five_prime_utr;Parent=Msa1448630-mRNA-1
tig0032236 CDS 36998 37109 36998 ID=Msa1448630-mRNA-1:cds;Parent=Msa1448630-mRNA-1
tig0032236 CDS 36760 36887 36760 ID=Msa1448630-mRNA-1:cds;Parent=Msa1448630-mRNA-1
tig0032236 three_prime_UTR 36152 36759 36152 ID=Msa1448630-mRNA-1:three_prime_utr;Parent=Msa1448630-mRNA-1
Gene Sequence

>Msa1448630

ATGAGTCCAAACAAGCCTCATGTTCTCATATTACTAATCATCATGCTTGTTTCAGTTTCTAATAAACCATCACTTATAGAAGCAAGGACACTTTCCTTAATTTCAAACCAAGGGTACTCAAAGATTTTTGCAACACTTGGAGTTGTTTGCAAATGTTGTGATGGAATTGGAGGTACTTGCACTAGTACTTGGACACAATCTTGTAATAACTTAAAGTGTTCACCTTGGAAATCACACTAG

Protein sequence

>Msa1448630

MSPNKPHVLILLIIMLVSVSNKPSLIEARTLSLISNQGYSKIFATLGVVCKCCDGIGGTCTSTWTQSCNNLKCSPWKSH*