AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0474870

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0474870 MtrunA17_Chr3g0131951 96.753 308 9 1 1 307 63 370 0.0 609
Msa0474870 MtrunA17_Chr8g0386001 64.017 239 86 0 10 248 10 248 6.71e-115 333
Msa0474870 MtrunA17_Chr8g0386011 52.321 237 108 3 1 233 1 236 3.60e-85 256
Msa0474870 MtrunA17_Chr7g0214181 52.941 238 107 2 1 238 1 233 1.46e-81 248
Msa0474870 MtrunA17_Chr7g0214191 46.454 282 133 4 1 282 1 264 2.87e-81 247
Msa0474870 MtrunA17_Chr5g0428281 47.464 276 135 4 1 275 1 267 1.17e-79 243
Msa0474870 MtrunA17_Chr3g0131961 48.052 231 117 3 4 233 5 233 2.09e-75 232
Msa0474870 MtrunA17_Chr7g0215601 50.218 229 112 2 1 228 1 228 2.14e-71 221
Msa0474870 MtrunA17_Chr5g0444031 45.082 244 129 2 8 247 28 270 1.65e-68 214
Msa0474870 MtrunA17_Chr2g0295681 41.148 209 122 1 9 217 5 212 2.74e-49 164
Msa0474870 MtrunA17_Chr2g0278491 40.179 224 126 5 56 275 1 220 1.38e-47 159
Msa0474870 MtrunA17_Chr3g0119951 36.179 246 150 5 11 252 10 252 6.61e-44 150
Msa0474870 MtrunA17_Chr3g0125861 36.161 224 137 4 4 225 2 221 7.27e-42 145
Msa0474870 MtrunA17_Chr8g0388621 33.465 254 160 5 1 251 1 248 2.39e-41 144
Msa0474870 MtrunA17_Chr1g0158931 36.986 219 133 3 8 222 5 222 7.69e-41 142
Msa0474870 MtrunA17_Chr3g0119991 36.481 233 141 5 24 252 2 231 1.07e-40 142
Msa0474870 MtrunA17_Chr6g0450941 38.426 216 127 4 13 223 85 299 2.17e-40 143
Msa0474870 MtrunA17_Chr1g0159431 37.220 223 130 4 10 228 6 222 5.64e-40 140
Msa0474870 MtrunA17_Chr3g0126421 36.255 251 146 9 8 250 5 249 7.90e-37 132
Msa0474870 MtrunA17_Chr3g0126411 35.458 251 148 8 8 250 5 249 2.67e-36 130
Msa0474870 MtrunA17_Chr8g0359261 36.967 211 125 3 11 217 16 222 3.74e-36 130
Msa0474870 MtrunA17_Chr4g0059191 33.014 209 137 2 25 231 1 208 1.20e-35 127
Msa0474870 MtrunA17_Chr6g0450951 39.062 192 113 3 29 217 5 195 1.73e-34 125
Msa0474870 MtrunA17_Chr2g0314571 34.562 217 138 2 11 225 16 230 1.49e-33 123
Msa0474870 MtrunA17_Chr6g0450931 36.000 200 122 4 29 223 5 203 1.73e-32 119
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0474870 AT5G23660.1 55.686 255 93 3 5 259 7 241 1.98e-94 281
Msa0474870 AT5G50800.1 51.014 296 131 6 1 288 1 290 7.89e-94 280
Msa0474870 AT3G48740.1 49.827 289 134 4 9 296 11 289 1.80e-90 271
Msa0474870 AT4G25010.1 50.719 278 131 4 5 279 5 279 6.82e-89 267
Msa0474870 AT5G13170.1 45.724 304 148 6 1 304 4 290 1.48e-87 264
Msa0474870 AT5G50790.1 43.197 294 153 5 1 285 1 289 4.44e-81 247
Msa0474870 AT2G39060.1 42.972 249 122 2 8 237 8 255 3.72e-65 206
Msa0474870 AT4G15920.1 36.967 211 133 0 9 219 5 215 1.97e-51 170
Msa0474870 AT3G16690.3 37.220 223 138 2 2 223 33 254 3.40e-51 170
Msa0474870 AT3G16690.1 36.986 219 136 2 6 223 2 219 2.52e-50 166
Msa0474870 AT1G21460.1 40.408 245 130 6 12 254 8 238 1.66e-47 160
Msa0474870 AT4G10850.1 35.268 224 142 2 3 223 4 227 1.13e-43 150
Msa0474870 AT1G66770.1 32.489 237 158 1 13 247 14 250 1.66e-40 142
Msa0474870 AT5G40260.1 39.631 217 125 5 7 218 6 221 2.04e-39 139
Msa0474870 AT5G53190.1 31.119 286 160 8 8 284 5 262 2.98e-39 139
Msa0474870 AT5G62850.1 33.790 219 139 4 1 216 1 216 5.45e-39 137
Msa0474870 AT1G21460.2 43.243 185 101 3 12 194 8 190 3.70e-37 132
Msa0474870 AT3G14770.1 35.945 217 131 4 13 226 18 229 8.75e-35 126
Msa0474870 AT3G28007.1 38.095 210 123 4 13 218 12 218 1.49e-34 126
Msa0474870 AT5G40260.2 40.838 191 107 5 7 192 6 195 3.08e-33 122
Msa0474870 AT5G62850.2 34.973 183 114 3 13 192 12 192 2.83e-32 119
Msa0474870 AT3G16690.2 32.812 192 113 3 6 182 2 192 3.48e-27 105

Find 76 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACATCATTTCTTTGCTTTC+TGG 0.098038 3_3:+77910114 Msa0474870:CDS
CTCAATTCTAGACAAGTTTA+TGG 0.157039 3_3:+77911940 Msa0474870:three_prime_UTR
GAAGGAAGATTTAATTTAAA+AGG 0.168383 3_3:-77909966 None:intergenic
AAGAAGAGTTTCACCCTTCT+TGG 0.240770 3_3:-77910350 None:intergenic
GCACTCACAATAATGCTTAA+AGG 0.256613 3_3:-77910834 None:intergenic
TACACGTACCCTAACTTTCT+TGG 0.259331 3_3:-77910440 None:intergenic
TTATGGAAATTATTATCTTA+TGG 0.274592 3_3:+77911957 Msa0474870:three_prime_UTR
GGGCATATATTATTATAATT+AGG 0.291098 3_3:-77911918 None:intergenic
TACAACTTGTGGGTTAATAT+TGG 0.291724 3_3:-77912023 None:intergenic
CTTACTGCCCCAAGAAAGTT+AGG 0.295172 3_3:+77910431 Msa0474870:CDS
TCTTTCGCCTTTGGAGTTCT+AGG 0.295855 3_3:+77910085 Msa0474870:CDS
ACACGTACCCTAACTTTCTT+GGG 0.302349 3_3:-77910439 None:intergenic
TTGATGAACTTTGTGGGATT+TGG 0.311697 3_3:+77910708 Msa0474870:CDS
TATTGTGACTGTAGAGAAAA+AGG 0.313551 3_3:+77911556 Msa0474870:CDS
AGTTTCACCCTTCTTGGTAT+AGG 0.322134 3_3:-77910344 None:intergenic
AACGTTGTGGGGTTAACATT+TGG 0.325987 3_3:+77911371 Msa0474870:CDS
AGGAAGGAACTCTACACTTT+TGG 0.334515 3_3:-77910987 None:intergenic
TGGTAGCTTCTCATCAATTA+CGG 0.338030 3_3:-77911438 None:intergenic
CACTCACAATAATGCTTAAA+GGG 0.349067 3_3:-77910833 None:intergenic
AGTCATCTATCTTTCGCCTT+TGG 0.350886 3_3:+77910076 Msa0474870:CDS
CACTATTTAGTGCAATGCTT+TGG 0.369787 3_3:+77910311 Msa0474870:CDS
CACACAACCGAATGCATTGA+TGG 0.370212 3_3:-77910377 None:intergenic
GGTAGCTTCTCATCAATTAC+GGG 0.374020 3_3:-77911437 None:intergenic
TTGATGTACTCTAAAGATTA+CGG 0.375925 3_3:+77911985 Msa0474870:three_prime_UTR
GAAGGACGTATCAAGCTTCT+TGG 0.381738 3_3:+77910771 Msa0474870:CDS
AATGTTAACCCCACAACGTT+CGG 0.388997 3_3:-77911368 None:intergenic
CATAAACTTGTCTAGAATTG+AGG 0.394159 3_3:-77911939 None:intergenic
ACAATGTCTCCTTTATGTTC+TGG 0.402350 3_3:-77911458 None:intergenic
CTAAATAGTGCAGCCACATA+TGG 0.415521 3_3:-77910298 None:intergenic
CTTATCACCATCAATGCATT+CGG 0.422646 3_3:+77910370 Msa0474870:CDS
AGGAGAAGTGAAAGTGGAAA+AGG 0.437747 3_3:-77911007 None:intergenic
ATGTTAACCCCACAACGTTC+GGG 0.444928 3_3:-77911367 None:intergenic
ATAAACTTGTCTAGAATTGA+GGG 0.446053 3_3:-77911938 None:intergenic
GGACAGCTCCCGAACGTTGT+GGG 0.447921 3_3:+77911359 Msa0474870:intron
TCCTTCTCTCCCTTCTCACA+AGG 0.455581 3_3:+77909983 Msa0474870:five_prime_UTR
CAGCCACATATGGAATTGAT+TGG 0.459983 3_3:-77910288 None:intergenic
TGATGAGAATGAGAAGAAAC+AGG 0.461226 3_3:+77911601 Msa0474870:CDS
TTACAAATTCGATAAAATGT+TGG 0.461818 3_3:-77910244 None:intergenic
TCCTCATAAGCGCGGTTATG+TGG 0.463599 3_3:+77911029 Msa0474870:CDS
GATTGTGTTGATGAACTTTG+TGG 0.471961 3_3:+77910701 Msa0474870:CDS
ATGAGGAGGAGAAGTGAAAG+TGG 0.474274 3_3:-77911013 None:intergenic
TTTCCAATCAATTCCATATG+TGG 0.478701 3_3:+77910285 Msa0474870:CDS
CCCTTGTGAGAAGGGAGAGA+AGG 0.480289 3_3:-77909984 None:intergenic
GCCACATAACCGCGCTTATG+AGG 0.482912 3_3:-77911030 None:intergenic
GAGAAGCTACCAGAACATAA+AGG 0.487420 3_3:+77911449 Msa0474870:CDS
TCTCTGTTTACATCTACTTG+TGG 0.498460 3_3:+77911837 Msa0474870:three_prime_UTR
GTTAGTGGTTGAGAAATAGT+TGG 0.504921 3_3:+77911698 Msa0474870:exon
CTGAATTATCCCTTGTGAGA+AGG 0.505869 3_3:-77909993 None:intergenic
GCGGTTATGTGGCTGTTGTA+TGG 0.506943 3_3:+77911040 Msa0474870:CDS
TGAATTATCCCTTGTGAGAA+GGG 0.510719 3_3:-77909992 None:intergenic
TGGACAGCTCCCGAACGTTG+TGG 0.525233 3_3:+77911358 Msa0474870:intron
AAAACAGAAGAGAAGGTTAG+TGG 0.526127 3_3:+77911683 Msa0474870:CDS
TTACAGTAACATAGATATCT+CGG 0.536034 3_3:-77911072 None:intergenic
ATTGTGTTGATGAACTTTGT+GGG 0.543350 3_3:+77910702 Msa0474870:CDS
CGCATATAAAGGAGAAGAGA+CGG 0.552967 3_3:+77911788 Msa0474870:three_prime_UTR
GACGTATCAAGCTTCTTGGA+TGG 0.557609 3_3:+77910775 Msa0474870:CDS
CCTTCTCTCCCTTCTCACAA+GGG 0.557625 3_3:+77909984 Msa0474870:five_prime_UTR
TATCAACAAAACAGAAGAGA+AGG 0.559044 3_3:+77911676 Msa0474870:CDS
TTCCTAGCAAAACAAGAAGA+AGG 0.574175 3_3:+77910753 Msa0474870:CDS
TGTAAGAAGAAATCAACAGA+AGG 0.576661 3_3:+77910262 Msa0474870:CDS
GTGGTTGAGAAATAGTTGGC+GGG 0.578004 3_3:+77911702 Msa0474870:exon
GACAGCTCCCGAACGTTGTG+GGG 0.582179 3_3:+77911360 Msa0474870:intron
AGTGGTTGAGAAATAGTTGG+CGG 0.598698 3_3:+77911701 Msa0474870:exon
TTATGTAGATGTCAACATTG+AGG 0.602218 3_3:+77910676 Msa0474870:intron
GAAGTGAAAGTGGAAAAGGA+AGG 0.605323 3_3:-77911003 None:intergenic
GAAAGATAGATGACTGTGGT+GGG 0.607987 3_3:-77910068 None:intergenic
CGAAAGATAGATGACTGTGG+TGG 0.609394 3_3:-77910069 None:intergenic
ATGACTGTGGTGGGACGACA+TGG 0.619238 3_3:-77910059 None:intergenic
ACATAACCGCGCTTATGAGG+AGG 0.633283 3_3:-77911027 None:intergenic
TTACTGCCCCAAGAAAGTTA+GGG 0.638169 3_3:+77910432 Msa0474870:CDS
ACTTCTCCTCCTCATAAGCG+CGG 0.645264 3_3:+77911021 Msa0474870:CDS
AATGTTGCCTAGAACTCCAA+AGG 0.654062 3_3:-77910092 None:intergenic
CACGTACCCTAACTTTCTTG+GGG 0.660388 3_3:-77910438 None:intergenic
ACTCACAATAATGCTTAAAG+GGG 0.675502 3_3:-77910832 None:intergenic
AGTAACTCAAGACAAAACAA+AGG 0.690695 3_3:+77911628 Msa0474870:CDS
AGGCGAAAGATAGATGACTG+TGG 0.756668 3_3:-77910072 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTTACAATTAAATGAAG+TGG - chr3_3:77910174-77910193 None:intergenic 15.0%
!! AAAATAATTAATGTTGATCA+TGG - chr3_3:77910645-77910664 None:intergenic 15.0%
!! AAAATCAATACTACTAAATT+TGG + chr3_3:77911134-77911153 Msa0474870:intron 15.0%
!! CATTTGTATTAATTAATTTC+TGG + chr3_3:77911280-77911299 Msa0474870:intron 15.0%
!!! CAAAATCTTATGTTTTTTTA+TGG + chr3_3:77911338-77911357 Msa0474870:intron 15.0%
!! TTATGGAAATTATTATCTTA+TGG + chr3_3:77911957-77911976 Msa0474870:three_prime_UTR 15.0%
!! AAATTTACAATTAAATGAAG+TGG - chr3_3:77910174-77910193 None:intergenic 15.0%
!! AAAATAATTAATGTTGATCA+TGG - chr3_3:77910645-77910664 None:intergenic 15.0%
!! AAAATCAATACTACTAAATT+TGG + chr3_3:77911134-77911153 Msa0474870:intron 15.0%
!! CATTTGTATTAATTAATTTC+TGG + chr3_3:77911280-77911299 Msa0474870:intron 15.0%
!!! CAAAATCTTATGTTTTTTTA+TGG + chr3_3:77911338-77911357 Msa0474870:intron 15.0%
!! TTATGGAAATTATTATCTTA+TGG + chr3_3:77911957-77911976 Msa0474870:three_prime_UTR 15.0%
!!! GAAGGAAGATTTAATTTAAA+AGG - chr3_3:77909969-77909988 None:intergenic 20.0%
!! TTACAAATTCGATAAAATGT+TGG - chr3_3:77910247-77910266 None:intergenic 20.0%
!! GGGCATATATTATTATAATT+AGG - chr3_3:77911921-77911940 None:intergenic 20.0%
!!! GAAGGAAGATTTAATTTAAA+AGG - chr3_3:77909969-77909988 None:intergenic 20.0%
!! TTACAAATTCGATAAAATGT+TGG - chr3_3:77910247-77910266 None:intergenic 20.0%
!! GGGCATATATTATTATAATT+AGG - chr3_3:77911921-77911940 None:intergenic 20.0%
! ACAAAAATTAAGCTAGAGTT+TGG + chr3_3:77909939-77909958 Msa0474870:five_prime_UTR 25.0%
! ATTATTCTTTCTACTTACAG+TGG - chr3_3:77910142-77910161 None:intergenic 25.0%
! TTTAACATTCCAACAATGTT+GGG - chr3_3:77910509-77910528 None:intergenic 25.0%
! ATTTAACATTCCAACAATGT+TGG - chr3_3:77910510-77910529 None:intergenic 25.0%
!!! TTTTTTTTGACAAAGTTGCT+AGG + chr3_3:77910537-77910556 Msa0474870:intron 25.0%
! TTACAGTAACATAGATATCT+CGG - chr3_3:77911075-77911094 None:intergenic 25.0%
!!! TAGTTGCTATTTTGTCTTTA+TGG + chr3_3:77911303-77911322 Msa0474870:intron 25.0%
!!! ATATTTTGAGACGCATATAA+AGG + chr3_3:77911777-77911796 Msa0474870:three_prime_UTR 25.0%
!! ATAAACTTGTCTAGAATTGA+GGG - chr3_3:77911941-77911960 None:intergenic 25.0%
!! TTGATGTACTCTAAAGATTA+CGG + chr3_3:77911985-77912004 Msa0474870:three_prime_UTR 25.0%
! ACAAAAATTAAGCTAGAGTT+TGG + chr3_3:77909939-77909958 Msa0474870:five_prime_UTR 25.0%
! ATTATTCTTTCTACTTACAG+TGG - chr3_3:77910142-77910161 None:intergenic 25.0%
! TTTAACATTCCAACAATGTT+GGG - chr3_3:77910509-77910528 None:intergenic 25.0%
! ATTTAACATTCCAACAATGT+TGG - chr3_3:77910510-77910529 None:intergenic 25.0%
!!! TTTTTTTTGACAAAGTTGCT+AGG + chr3_3:77910537-77910556 Msa0474870:intron 25.0%
! TTACAGTAACATAGATATCT+CGG - chr3_3:77911075-77911094 None:intergenic 25.0%
!!! TAGTTGCTATTTTGTCTTTA+TGG + chr3_3:77911303-77911322 Msa0474870:intron 25.0%
!!! ATATTTTGAGACGCATATAA+AGG + chr3_3:77911777-77911796 Msa0474870:three_prime_UTR 25.0%
!! ATAAACTTGTCTAGAATTGA+GGG - chr3_3:77911941-77911960 None:intergenic 25.0%
!! TTGATGTACTCTAAAGATTA+CGG + chr3_3:77911985-77912004 Msa0474870:three_prime_UTR 25.0%
TACATCATTTCTTTGCTTTC+TGG + chr3_3:77910114-77910133 Msa0474870:CDS 30.0%
TGTAAGAAGAAATCAACAGA+AGG + chr3_3:77910262-77910281 Msa0474870:CDS 30.0%
TTTCCAATCAATTCCATATG+TGG + chr3_3:77910285-77910304 Msa0474870:CDS 30.0%
! TTTTATGCCTATACCAAGAA+GGG + chr3_3:77910337-77910356 Msa0474870:CDS 30.0%
TTATGTAGATGTCAACATTG+AGG + chr3_3:77910676-77910695 Msa0474870:intron 30.0%
! ATTGTGTTGATGAACTTTGT+GGG + chr3_3:77910702-77910721 Msa0474870:CDS 30.0%
ACTCACAATAATGCTTAAAG+GGG - chr3_3:77910835-77910854 None:intergenic 30.0%
CACTCACAATAATGCTTAAA+GGG - chr3_3:77910836-77910855 None:intergenic 30.0%
AAAAGCTAATTCTAGCATGT+CGG - chr3_3:77910875-77910894 None:intergenic 30.0%
AGGATGATTAAAATCATGCA+TGG - chr3_3:77910947-77910966 None:intergenic 30.0%
!!! TCATCATTTGTTGTTTTGCT+TGG - chr3_3:77911512-77911531 None:intergenic 30.0%
TATTGTGACTGTAGAGAAAA+AGG + chr3_3:77911556-77911575 Msa0474870:CDS 30.0%
!! TTGTTTTGTCTTGAGTTACT+TGG - chr3_3:77911628-77911647 None:intergenic 30.0%
AGTAACTCAAGACAAAACAA+AGG + chr3_3:77911628-77911647 Msa0474870:CDS 30.0%
TATCAACAAAACAGAAGAGA+AGG + chr3_3:77911676-77911695 Msa0474870:CDS 30.0%
! CATAAACTTGTCTAGAATTG+AGG - chr3_3:77911942-77911961 None:intergenic 30.0%
CTCAATTCTAGACAAGTTTA+TGG + chr3_3:77911940-77911959 Msa0474870:three_prime_UTR 30.0%
TACATCATTTCTTTGCTTTC+TGG + chr3_3:77910114-77910133 Msa0474870:CDS 30.0%
TGTAAGAAGAAATCAACAGA+AGG + chr3_3:77910262-77910281 Msa0474870:CDS 30.0%
TTTCCAATCAATTCCATATG+TGG + chr3_3:77910285-77910304 Msa0474870:CDS 30.0%
! TTTTATGCCTATACCAAGAA+GGG + chr3_3:77910337-77910356 Msa0474870:CDS 30.0%
TTATGTAGATGTCAACATTG+AGG + chr3_3:77910676-77910695 Msa0474870:intron 30.0%
! ATTGTGTTGATGAACTTTGT+GGG + chr3_3:77910702-77910721 Msa0474870:CDS 30.0%
ACTCACAATAATGCTTAAAG+GGG - chr3_3:77910835-77910854 None:intergenic 30.0%
CACTCACAATAATGCTTAAA+GGG - chr3_3:77910836-77910855 None:intergenic 30.0%
AAAAGCTAATTCTAGCATGT+CGG - chr3_3:77910875-77910894 None:intergenic 30.0%
AGGATGATTAAAATCATGCA+TGG - chr3_3:77910947-77910966 None:intergenic 30.0%
!!! TCATCATTTGTTGTTTTGCT+TGG - chr3_3:77911512-77911531 None:intergenic 30.0%
TATTGTGACTGTAGAGAAAA+AGG + chr3_3:77911556-77911575 Msa0474870:CDS 30.0%
!! TTGTTTTGTCTTGAGTTACT+TGG - chr3_3:77911628-77911647 None:intergenic 30.0%
AGTAACTCAAGACAAAACAA+AGG + chr3_3:77911628-77911647 Msa0474870:CDS 30.0%
TATCAACAAAACAGAAGAGA+AGG + chr3_3:77911676-77911695 Msa0474870:CDS 30.0%
! CATAAACTTGTCTAGAATTG+AGG - chr3_3:77911942-77911961 None:intergenic 30.0%
CTCAATTCTAGACAAGTTTA+TGG + chr3_3:77911940-77911959 Msa0474870:three_prime_UTR 30.0%
TGAATTATCCCTTGTGAGAA+GGG - chr3_3:77909995-77910014 None:intergenic 35.0%
CACTATTTAGTGCAATGCTT+TGG + chr3_3:77910311-77910330 Msa0474870:CDS 35.0%
! GTTTTATGCCTATACCAAGA+AGG + chr3_3:77910336-77910355 Msa0474870:CDS 35.0%
CTTATCACCATCAATGCATT+CGG + chr3_3:77910370-77910389 Msa0474870:CDS 35.0%
AGTAAGTGACAAAAACTGCA+AGG - chr3_3:77910417-77910436 None:intergenic 35.0%
! GATTGTGTTGATGAACTTTG+TGG + chr3_3:77910701-77910720 Msa0474870:CDS 35.0%
TTGATGAACTTTGTGGGATT+TGG + chr3_3:77910708-77910727 Msa0474870:CDS 35.0%
TTCCTAGCAAAACAAGAAGA+AGG + chr3_3:77910753-77910772 Msa0474870:CDS 35.0%
GCACTCACAATAATGCTTAA+AGG - chr3_3:77910837-77910856 None:intergenic 35.0%
TGGTAGCTTCTCATCAATTA+CGG - chr3_3:77911441-77911460 None:intergenic 35.0%
ACAATGTCTCCTTTATGTTC+TGG - chr3_3:77911461-77911480 None:intergenic 35.0%
TGATGAGAATGAGAAGAAAC+AGG + chr3_3:77911601-77911620 Msa0474870:CDS 35.0%
AAAACAGAAGAGAAGGTTAG+TGG + chr3_3:77911683-77911702 Msa0474870:CDS 35.0%
GTTAGTGGTTGAGAAATAGT+TGG + chr3_3:77911698-77911717 Msa0474870:exon 35.0%
TCTCTGTTTACATCTACTTG+TGG + chr3_3:77911837-77911856 Msa0474870:three_prime_UTR 35.0%
TGAATTATCCCTTGTGAGAA+GGG - chr3_3:77909995-77910014 None:intergenic 35.0%
CACTATTTAGTGCAATGCTT+TGG + chr3_3:77910311-77910330 Msa0474870:CDS 35.0%
! GTTTTATGCCTATACCAAGA+AGG + chr3_3:77910336-77910355 Msa0474870:CDS 35.0%
CTTATCACCATCAATGCATT+CGG + chr3_3:77910370-77910389 Msa0474870:CDS 35.0%
AGTAAGTGACAAAAACTGCA+AGG - chr3_3:77910417-77910436 None:intergenic 35.0%
! GATTGTGTTGATGAACTTTG+TGG + chr3_3:77910701-77910720 Msa0474870:CDS 35.0%
TTGATGAACTTTGTGGGATT+TGG + chr3_3:77910708-77910727 Msa0474870:CDS 35.0%
TTCCTAGCAAAACAAGAAGA+AGG + chr3_3:77910753-77910772 Msa0474870:CDS 35.0%
GCACTCACAATAATGCTTAA+AGG - chr3_3:77910837-77910856 None:intergenic 35.0%
TGGTAGCTTCTCATCAATTA+CGG - chr3_3:77911441-77911460 None:intergenic 35.0%
ACAATGTCTCCTTTATGTTC+TGG - chr3_3:77911461-77911480 None:intergenic 35.0%
TGATGAGAATGAGAAGAAAC+AGG + chr3_3:77911601-77911620 Msa0474870:CDS 35.0%
AAAACAGAAGAGAAGGTTAG+TGG + chr3_3:77911683-77911702 Msa0474870:CDS 35.0%
GTTAGTGGTTGAGAAATAGT+TGG + chr3_3:77911698-77911717 Msa0474870:exon 35.0%
TCTCTGTTTACATCTACTTG+TGG + chr3_3:77911837-77911856 Msa0474870:three_prime_UTR 35.0%
CTGAATTATCCCTTGTGAGA+AGG - chr3_3:77909996-77910015 None:intergenic 40.0%
GAAAGATAGATGACTGTGGT+GGG - chr3_3:77910071-77910090 None:intergenic 40.0%
AGTCATCTATCTTTCGCCTT+TGG + chr3_3:77910076-77910095 Msa0474870:CDS 40.0%
AATGTTGCCTAGAACTCCAA+AGG - chr3_3:77910095-77910114 None:intergenic 40.0%
CAGCCACATATGGAATTGAT+TGG - chr3_3:77910291-77910310 None:intergenic 40.0%
CTAAATAGTGCAGCCACATA+TGG - chr3_3:77910301-77910320 None:intergenic 40.0%
AGTTTCACCCTTCTTGGTAT+AGG - chr3_3:77910347-77910366 None:intergenic 40.0%
AAGAAGAGTTTCACCCTTCT+TGG - chr3_3:77910353-77910372 None:intergenic 40.0%
TTACTGCCCCAAGAAAGTTA+GGG + chr3_3:77910432-77910451 Msa0474870:CDS 40.0%
ACACGTACCCTAACTTTCTT+GGG - chr3_3:77910442-77910461 None:intergenic 40.0%
TACACGTACCCTAACTTTCT+TGG - chr3_3:77910443-77910462 None:intergenic 40.0%
AGCTAAAGTCCCAACATTGT+TGG + chr3_3:77910497-77910516 Msa0474870:intron 40.0%
!!! GTCCTTCTTCTTGTTTTGCT+AGG - chr3_3:77910758-77910777 None:intergenic 40.0%
! AGGAAGGAACTCTACACTTT+TGG - chr3_3:77910990-77911009 None:intergenic 40.0%
GAAGTGAAAGTGGAAAAGGA+AGG - chr3_3:77911006-77911025 None:intergenic 40.0%
AGGAGAAGTGAAAGTGGAAA+AGG - chr3_3:77911010-77911029 None:intergenic 40.0%
AATGTTAACCCCACAACGTT+CGG - chr3_3:77911371-77911390 None:intergenic 40.0%
AACGTTGTGGGGTTAACATT+TGG + chr3_3:77911371-77911390 Msa0474870:CDS 40.0%
GGTAGCTTCTCATCAATTAC+GGG - chr3_3:77911440-77911459 None:intergenic 40.0%
GAGAAGCTACCAGAACATAA+AGG + chr3_3:77911449-77911468 Msa0474870:CDS 40.0%
AGTGGTTGAGAAATAGTTGG+CGG + chr3_3:77911701-77911720 Msa0474870:exon 40.0%
CGCATATAAAGGAGAAGAGA+CGG + chr3_3:77911788-77911807 Msa0474870:three_prime_UTR 40.0%
CTGAATTATCCCTTGTGAGA+AGG - chr3_3:77909996-77910015 None:intergenic 40.0%
GAAAGATAGATGACTGTGGT+GGG - chr3_3:77910071-77910090 None:intergenic 40.0%
AGTCATCTATCTTTCGCCTT+TGG + chr3_3:77910076-77910095 Msa0474870:CDS 40.0%
AATGTTGCCTAGAACTCCAA+AGG - chr3_3:77910095-77910114 None:intergenic 40.0%
CAGCCACATATGGAATTGAT+TGG - chr3_3:77910291-77910310 None:intergenic 40.0%
CTAAATAGTGCAGCCACATA+TGG - chr3_3:77910301-77910320 None:intergenic 40.0%
AGTTTCACCCTTCTTGGTAT+AGG - chr3_3:77910347-77910366 None:intergenic 40.0%
AAGAAGAGTTTCACCCTTCT+TGG - chr3_3:77910353-77910372 None:intergenic 40.0%
TTACTGCCCCAAGAAAGTTA+GGG + chr3_3:77910432-77910451 Msa0474870:CDS 40.0%
ACACGTACCCTAACTTTCTT+GGG - chr3_3:77910442-77910461 None:intergenic 40.0%
TACACGTACCCTAACTTTCT+TGG - chr3_3:77910443-77910462 None:intergenic 40.0%
AGCTAAAGTCCCAACATTGT+TGG + chr3_3:77910497-77910516 Msa0474870:intron 40.0%
!!! GTCCTTCTTCTTGTTTTGCT+AGG - chr3_3:77910758-77910777 None:intergenic 40.0%
! AGGAAGGAACTCTACACTTT+TGG - chr3_3:77910990-77911009 None:intergenic 40.0%
GAAGTGAAAGTGGAAAAGGA+AGG - chr3_3:77911006-77911025 None:intergenic 40.0%
AGGAGAAGTGAAAGTGGAAA+AGG - chr3_3:77911010-77911029 None:intergenic 40.0%
AATGTTAACCCCACAACGTT+CGG - chr3_3:77911371-77911390 None:intergenic 40.0%
AACGTTGTGGGGTTAACATT+TGG + chr3_3:77911371-77911390 Msa0474870:CDS 40.0%
GGTAGCTTCTCATCAATTAC+GGG - chr3_3:77911440-77911459 None:intergenic 40.0%
GAGAAGCTACCAGAACATAA+AGG + chr3_3:77911449-77911468 Msa0474870:CDS 40.0%
AGTGGTTGAGAAATAGTTGG+CGG + chr3_3:77911701-77911720 Msa0474870:exon 40.0%
CGCATATAAAGGAGAAGAGA+CGG + chr3_3:77911788-77911807 Msa0474870:three_prime_UTR 40.0%
CGAAAGATAGATGACTGTGG+TGG - chr3_3:77910072-77910091 None:intergenic 45.0%
AGGCGAAAGATAGATGACTG+TGG - chr3_3:77910075-77910094 None:intergenic 45.0%
!! TCTTTCGCCTTTGGAGTTCT+AGG + chr3_3:77910085-77910104 Msa0474870:CDS 45.0%
CACACAACCGAATGCATTGA+TGG - chr3_3:77910380-77910399 None:intergenic 45.0%
CTTACTGCCCCAAGAAAGTT+AGG + chr3_3:77910431-77910450 Msa0474870:CDS 45.0%
CACGTACCCTAACTTTCTTG+GGG - chr3_3:77910441-77910460 None:intergenic 45.0%
GAAGGACGTATCAAGCTTCT+TGG + chr3_3:77910771-77910790 Msa0474870:CDS 45.0%
GACGTATCAAGCTTCTTGGA+TGG + chr3_3:77910775-77910794 Msa0474870:CDS 45.0%
TGCGAATAACAACTCTCTGC+AGG - chr3_3:77910967-77910986 None:intergenic 45.0%
ATGAGGAGGAGAAGTGAAAG+TGG - chr3_3:77911016-77911035 None:intergenic 45.0%
ATGTTAACCCCACAACGTTC+GGG - chr3_3:77911370-77911389 None:intergenic 45.0%
GTGGTTGAGAAATAGTTGGC+GGG + chr3_3:77911702-77911721 Msa0474870:exon 45.0%
CGAAAGATAGATGACTGTGG+TGG - chr3_3:77910072-77910091 None:intergenic 45.0%
AGGCGAAAGATAGATGACTG+TGG - chr3_3:77910075-77910094 None:intergenic 45.0%
!! TCTTTCGCCTTTGGAGTTCT+AGG + chr3_3:77910085-77910104 Msa0474870:CDS 45.0%
CACACAACCGAATGCATTGA+TGG - chr3_3:77910380-77910399 None:intergenic 45.0%
CTTACTGCCCCAAGAAAGTT+AGG + chr3_3:77910431-77910450 Msa0474870:CDS 45.0%
CACGTACCCTAACTTTCTTG+GGG - chr3_3:77910441-77910460 None:intergenic 45.0%
GAAGGACGTATCAAGCTTCT+TGG + chr3_3:77910771-77910790 Msa0474870:CDS 45.0%
GACGTATCAAGCTTCTTGGA+TGG + chr3_3:77910775-77910794 Msa0474870:CDS 45.0%
TGCGAATAACAACTCTCTGC+AGG - chr3_3:77910967-77910986 None:intergenic 45.0%
ATGAGGAGGAGAAGTGAAAG+TGG - chr3_3:77911016-77911035 None:intergenic 45.0%
ATGTTAACCCCACAACGTTC+GGG - chr3_3:77911370-77911389 None:intergenic 45.0%
GTGGTTGAGAAATAGTTGGC+GGG + chr3_3:77911702-77911721 Msa0474870:exon 45.0%
TCCTTCTCTCCCTTCTCACA+AGG + chr3_3:77909983-77910002 Msa0474870:five_prime_UTR 50.0%
CCTTCTCTCCCTTCTCACAA+GGG + chr3_3:77909984-77910003 Msa0474870:five_prime_UTR 50.0%
ACTTCTCCTCCTCATAAGCG+CGG + chr3_3:77911021-77911040 Msa0474870:CDS 50.0%
ACATAACCGCGCTTATGAGG+AGG - chr3_3:77911030-77911049 None:intergenic 50.0%
TCCTCATAAGCGCGGTTATG+TGG + chr3_3:77911029-77911048 Msa0474870:CDS 50.0%
GCGGTTATGTGGCTGTTGTA+TGG + chr3_3:77911040-77911059 Msa0474870:CDS 50.0%
TCCTTCTCTCCCTTCTCACA+AGG + chr3_3:77909983-77910002 Msa0474870:five_prime_UTR 50.0%
CCTTCTCTCCCTTCTCACAA+GGG + chr3_3:77909984-77910003 Msa0474870:five_prime_UTR 50.0%
ACTTCTCCTCCTCATAAGCG+CGG + chr3_3:77911021-77911040 Msa0474870:CDS 50.0%
ACATAACCGCGCTTATGAGG+AGG - chr3_3:77911030-77911049 None:intergenic 50.0%
TCCTCATAAGCGCGGTTATG+TGG + chr3_3:77911029-77911048 Msa0474870:CDS 50.0%
GCGGTTATGTGGCTGTTGTA+TGG + chr3_3:77911040-77911059 Msa0474870:CDS 50.0%
CCCTTGTGAGAAGGGAGAGA+AGG - chr3_3:77909987-77910006 None:intergenic 55.0%
! ATGACTGTGGTGGGACGACA+TGG - chr3_3:77910062-77910081 None:intergenic 55.0%
GCCACATAACCGCGCTTATG+AGG - chr3_3:77911033-77911052 None:intergenic 55.0%
CCCTTGTGAGAAGGGAGAGA+AGG - chr3_3:77909987-77910006 None:intergenic 55.0%
! ATGACTGTGGTGGGACGACA+TGG - chr3_3:77910062-77910081 None:intergenic 55.0%
GCCACATAACCGCGCTTATG+AGG - chr3_3:77911033-77911052 None:intergenic 55.0%
TGGACAGCTCCCGAACGTTG+TGG + chr3_3:77911358-77911377 Msa0474870:intron 60.0%
GGACAGCTCCCGAACGTTGT+GGG + chr3_3:77911359-77911378 Msa0474870:intron 60.0%
GACAGCTCCCGAACGTTGTG+GGG + chr3_3:77911360-77911379 Msa0474870:intron 60.0%
TGGACAGCTCCCGAACGTTG+TGG + chr3_3:77911358-77911377 Msa0474870:intron 60.0%
GGACAGCTCCCGAACGTTGT+GGG + chr3_3:77911359-77911378 Msa0474870:intron 60.0%
GACAGCTCCCGAACGTTGTG+GGG + chr3_3:77911360-77911379 Msa0474870:intron 60.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 77909922 77912031 77909922 ID=Msa0474870;Name=Msa0474870
chr3_3 mRNA 77909922 77912031 77909922 ID=Msa0474870-mRNA-1;Parent=Msa0474870;Name=Msa0474870-mRNA-1;_AED=0.19;_eAED=0.19;_QI=139|1|1|1|1|1|5|322|307
chr3_3 exon 77909922 77910143 77909922 ID=Msa0474870-mRNA-1:exon:20906;Parent=Msa0474870-mRNA-1
chr3_3 exon 77910243 77910453 77910243 ID=Msa0474870-mRNA-1:exon:20907;Parent=Msa0474870-mRNA-1
chr3_3 exon 77910684 77910848 77910684 ID=Msa0474870-mRNA-1:exon:20908;Parent=Msa0474870-mRNA-1
chr3_3 exon 77910971 77911090 77910971 ID=Msa0474870-mRNA-1:exon:20909;Parent=Msa0474870-mRNA-1
chr3_3 exon 77911365 77912031 77911365 ID=Msa0474870-mRNA-1:exon:20910;Parent=Msa0474870-mRNA-1
chr3_3 five_prime_UTR 77909922 77910060 77909922 ID=Msa0474870-mRNA-1:five_prime_utr;Parent=Msa0474870-mRNA-1
chr3_3 CDS 77910061 77910143 77910061 ID=Msa0474870-mRNA-1:cds;Parent=Msa0474870-mRNA-1
chr3_3 CDS 77910243 77910453 77910243 ID=Msa0474870-mRNA-1:cds;Parent=Msa0474870-mRNA-1
chr3_3 CDS 77910684 77910848 77910684 ID=Msa0474870-mRNA-1:cds;Parent=Msa0474870-mRNA-1
chr3_3 CDS 77910971 77911090 77910971 ID=Msa0474870-mRNA-1:cds;Parent=Msa0474870-mRNA-1
chr3_3 CDS 77911365 77911709 77911365 ID=Msa0474870-mRNA-1:cds;Parent=Msa0474870-mRNA-1
chr3_3 three_prime_UTR 77911710 77912031 77911710 ID=Msa0474870-mRNA-1:three_prime_utr;Parent=Msa0474870-mRNA-1
Gene Sequence

>Msa0474870

ATGTCGTCCCACCACAGTCATCTATCTTTCGCCTTTGGAGTTCTAGGCAACATTACATCATTTCTTTGCTTTCTGGCACCACTACCAACATTTTATCGAATTTGTAAGAAGAAATCAACAGAAGGTTTCCAATCAATTCCATATGTGGCTGCACTATTTAGTGCAATGCTTTGGATGTTTTATGCCTATACCAAGAAGGGTGAAACTCTTCTTATCACCATCAATGCATTCGGTTGTGTGATAGAGACAATTTACCTTGCAGTTTTTGTCACTTACTGCCCCAAGAAAGTTAGGATGTCAACATTGAGGATGATTGTGTTGATGAACTTTGTGGGATTTGGCACGATTGTCTTGTTAACTCACTTCCTAGCAAAACAAGAAGAAGGACGTATCAAGCTTCTTGGATGGATTTGTGTAGTTTTTGCAACTAGTGTTTTTGCTGCCCCTTTAAGCATTATTAGAGTTGTTATTCGCACCAAAAGTGTAGAGTTCCTTCCTTTTCCACTTTCACTTCTCCTCCTCATAAGCGCGGTTATGTGGCTGTTGTATGGTCTTTCTCTCCGAGATATCTATGTTACTCTCCCGAACGTTGTGGGGTTAACATTTGGAATAGTTCAGATCACACTCTATGCAATGTACAGAAACAGCAAACCCGTAATTGATGAGAAGCTACCAGAACATAAAGGAGACATTGTCGACAAAGAAATTGAAAACGTAGTTGTTCCAAGCAAAACAACAAATGATGAGAAGAAACTAGAAGTGAATGTTGATATTGTGACTGTAGAGAAAAAGGAAGAGAAACAAGATGAAGAACATGATGAGAATGAGAAGAAACAGGACCAAGTAACTCAAGACAAAACAAAGGTTAAGAATGAAAATGACAACACTAATATCAACAAAACAGAAGAGAAGGTTAGTGGTTGA
Protein sequence

>Msa0474870

MSSHHSHLSFAFGVLGNITSFLCFLAPLPTFYRICKKKSTEGFQSIPYVAALFSAMLWMFYAYTKKGETLLITINAFGCVIETIYLAVFVTYCPKKVRMSTLRMIVLMNFVGFGTIVLLTHFLAKQEEGRIKLLGWICVVFATSVFAAPLSIIRVVIRTKSVEFLPFPLSLLLLISAVMWLLYGLSLRDIYVTLPNVVGLTFGIVQITLYAMYRNSKPVIDEKLPEHKGDIVDKEIENVVVPSKTTNDEKKLEVNVDIVTVEKKEEKQDEEHDENEKKQDQVTQDKTKVKNENDNTNINKTEEKVSG*