AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0474890

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0474890 MtrunA17_Chr3g0131951 97.078 308 8 1 1 307 63 370 0.0 610
Msa0474890 MtrunA17_Chr8g0386001 64.017 239 86 0 10 248 10 248 8.82e-115 332
Msa0474890 MtrunA17_Chr8g0386011 52.321 237 108 3 1 233 1 236 4.47e-85 256
Msa0474890 MtrunA17_Chr7g0214181 52.941 238 107 2 1 238 1 233 1.31e-81 248
Msa0474890 MtrunA17_Chr7g0214191 46.454 282 133 4 1 282 1 264 3.56e-81 246
Msa0474890 MtrunA17_Chr5g0428281 47.464 276 135 4 1 275 1 267 1.26e-79 243
Msa0474890 MtrunA17_Chr3g0131961 48.052 231 117 3 4 233 5 233 2.03e-75 232
Msa0474890 MtrunA17_Chr7g0215601 50.218 229 112 2 1 228 1 228 2.21e-71 221
Msa0474890 MtrunA17_Chr5g0444031 45.082 244 129 2 8 247 28 270 1.51e-68 214
Msa0474890 MtrunA17_Chr2g0295681 41.148 209 122 1 9 217 5 212 3.02e-49 164
Msa0474890 MtrunA17_Chr2g0278491 40.179 224 126 5 56 275 1 220 1.18e-47 159
Msa0474890 MtrunA17_Chr3g0119951 36.179 246 150 5 11 252 10 252 6.82e-44 150
Msa0474890 MtrunA17_Chr3g0125861 36.161 224 137 4 4 225 2 221 8.27e-42 145
Msa0474890 MtrunA17_Chr8g0388621 33.465 254 160 5 1 251 1 248 1.99e-41 144
Msa0474890 MtrunA17_Chr1g0158931 36.986 219 133 3 8 222 5 222 6.35e-41 142
Msa0474890 MtrunA17_Chr3g0119991 36.481 233 141 5 24 252 2 231 1.26e-40 141
Msa0474890 MtrunA17_Chr6g0450941 38.426 216 127 4 13 223 85 299 3.21e-40 142
Msa0474890 MtrunA17_Chr1g0159431 37.220 223 130 4 10 228 6 222 5.76e-40 140
Msa0474890 MtrunA17_Chr3g0126421 36.255 251 146 9 8 250 5 249 7.74e-37 132
Msa0474890 MtrunA17_Chr3g0126411 35.458 251 148 8 8 250 5 249 3.00e-36 130
Msa0474890 MtrunA17_Chr8g0359261 36.967 211 125 3 11 217 16 222 3.33e-36 130
Msa0474890 MtrunA17_Chr4g0059191 33.014 209 137 2 25 231 1 208 9.89e-36 128
Msa0474890 MtrunA17_Chr6g0450951 39.062 192 113 3 29 217 5 195 1.61e-34 125
Msa0474890 MtrunA17_Chr2g0314571 34.562 217 138 2 11 225 16 230 1.46e-33 123
Msa0474890 MtrunA17_Chr6g0450931 36.000 200 122 4 29 223 5 203 1.70e-32 119
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0474890 AT5G23660.1 55.686 255 93 3 5 259 7 241 1.91e-94 281
Msa0474890 AT5G50800.1 51.014 296 131 6 1 288 1 290 7.64e-94 280
Msa0474890 AT3G48740.1 50.534 281 125 4 9 281 11 285 2.09e-90 271
Msa0474890 AT4G25010.1 50.719 278 131 4 5 279 5 279 6.82e-89 267
Msa0474890 AT5G13170.1 45.695 302 149 6 1 302 4 290 1.84e-87 264
Msa0474890 AT5G50790.1 43.197 294 153 5 1 285 1 289 5.23e-81 247
Msa0474890 AT2G39060.1 42.972 249 122 2 8 237 8 255 3.60e-65 206
Msa0474890 AT4G15920.1 36.967 211 133 0 9 219 5 215 1.42e-51 170
Msa0474890 AT3G16690.3 37.220 223 138 2 2 223 33 254 2.99e-51 170
Msa0474890 AT3G16690.1 36.986 219 136 2 6 223 2 219 2.96e-50 166
Msa0474890 AT1G21460.1 40.408 245 130 6 12 254 8 238 1.49e-47 160
Msa0474890 AT4G10850.1 35.268 224 142 2 3 223 4 227 1.39e-43 150
Msa0474890 AT1G66770.1 32.489 237 158 1 13 247 14 250 1.59e-40 142
Msa0474890 AT5G40260.1 39.631 217 125 5 7 218 6 221 2.27e-39 139
Msa0474890 AT5G53190.1 31.119 286 160 8 8 284 5 262 3.28e-39 139
Msa0474890 AT5G62850.1 33.790 219 139 4 1 216 1 216 5.81e-39 137
Msa0474890 AT1G21460.2 43.243 185 101 3 12 194 8 190 3.50e-37 132
Msa0474890 AT3G14770.1 35.945 217 131 4 13 226 18 229 9.22e-35 126
Msa0474890 AT3G28007.1 38.095 210 123 4 13 218 12 218 1.48e-34 126
Msa0474890 AT5G40260.2 40.838 191 107 5 7 192 6 195 2.80e-33 122
Msa0474890 AT5G62850.2 34.973 183 114 3 13 192 12 192 2.83e-32 119
Msa0474890 AT3G16690.2 32.812 192 113 3 6 182 2 192 3.27e-27 105

Find 74 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCAATTCTAGACAAGTTTA+TGG 0.157039 3_3:+77930355 Msa0474890:three_prime_UTR
GAAGGAAGATTTAATTTAAA+AGG 0.168383 3_3:-77928380 None:intergenic
AAGAAGAGTTTCACCCTTCT+TGG 0.240770 3_3:-77928766 None:intergenic
TCTTTCGCATTTGGAGTTCT+AGG 0.241498 3_3:+77928501 Msa0474890:CDS
GCACTCACAATAATGCTTAA+AGG 0.256613 3_3:-77929249 None:intergenic
TACACGTACCCTAACTTTCT+TGG 0.259331 3_3:-77928856 None:intergenic
TTATGGAAATTATTATCTTA+TGG 0.274592 3_3:+77930372 Msa0474890:three_prime_UTR
GGGCATATATTATTATAATT+AGG 0.291098 3_3:-77930333 None:intergenic
TACAACTTGTGGGTTAATAT+TGG 0.291724 3_3:-77930442 None:intergenic
CTTACTGCCCCAAGAAAGTT+AGG 0.295172 3_3:+77928847 Msa0474890:CDS
ACACGTACCCTAACTTTCTT+GGG 0.302349 3_3:-77928855 None:intergenic
TTGATGAACTTTGTGGGATT+TGG 0.311697 3_3:+77929123 Msa0474890:CDS
TATTGTGACTGTAGAGAAAA+AGG 0.313551 3_3:+77929971 Msa0474890:CDS
AGTTTCACCCTTCTTGGTAT+AGG 0.322134 3_3:-77928760 None:intergenic
AACGTTGTGGGGTTAACATT+TGG 0.325987 3_3:+77929786 Msa0474890:CDS
AGGAAGGAACTCTACACTTT+TGG 0.334515 3_3:-77929402 None:intergenic
TGGTAGCTTCTCATCAATTA+CGG 0.338030 3_3:-77929853 None:intergenic
CACTCACAATAATGCTTAAA+GGG 0.349067 3_3:-77929248 None:intergenic
AGTCATCTATCTTTCGCATT+TGG 0.354656 3_3:+77928492 Msa0474890:CDS
CACTATTTAGTGCAATGCTT+TGG 0.369787 3_3:+77928727 Msa0474890:CDS
CACACAACCGAATGCATTGA+TGG 0.370212 3_3:-77928793 None:intergenic
GGTAGCTTCTCATCAATTAC+GGG 0.374020 3_3:-77929852 None:intergenic
TTGATGTACTCTAAAGATTA+CGG 0.375925 3_3:+77930404 Msa0474890:three_prime_UTR
GAAGGACGTATCAAGCTTCT+TGG 0.381738 3_3:+77929186 Msa0474890:CDS
AATGTTAACCCCACAACGTT+CGG 0.388997 3_3:-77929783 None:intergenic
CATAAACTTGTCTAGAATTG+AGG 0.394159 3_3:-77930354 None:intergenic
ACAATGTCTCCTTTATGTTC+TGG 0.403139 3_3:-77929873 None:intergenic
CTAAATAGTGCAGCCACATA+TGG 0.415521 3_3:-77928714 None:intergenic
CTTATCACCATCAATGCATT+CGG 0.422646 3_3:+77928786 Msa0474890:CDS
AGGAGAAGTGAAAGTGGAAA+AGG 0.437747 3_3:-77929422 None:intergenic
ATGTTAACCCCACAACGTTC+GGG 0.444928 3_3:-77929782 None:intergenic
ATAAACTTGTCTAGAATTGA+GGG 0.446053 3_3:-77930353 None:intergenic
GGACAGCTCCCGAACGTTGT+GGG 0.447921 3_3:+77929774 Msa0474890:intron
TCCTTCTCTCCCTTCTCACA+AGG 0.455581 3_3:+77928397 Msa0474890:five_prime_UTR
CAGCCACATATGGAATTGAT+TGG 0.459983 3_3:-77928704 None:intergenic
TGATGAGAATGAGAAGAAAC+AGG 0.461226 3_3:+77930016 Msa0474890:CDS
TTACAAATTCGATAAAATGT+TGG 0.461818 3_3:-77928660 None:intergenic
TCCTCATAAGCGCGGTTATG+TGG 0.463599 3_3:+77929444 Msa0474890:CDS
GATTGTGTTGATGAACTTTG+TGG 0.471961 3_3:+77929116 Msa0474890:CDS
ATGAGGAGGAGAAGTGAAAG+TGG 0.474274 3_3:-77929428 None:intergenic
TTTCCAATCAATTCCATATG+TGG 0.478701 3_3:+77928701 Msa0474890:CDS
CCCTTGTGAGAAGGGAGAGA+AGG 0.480289 3_3:-77928398 None:intergenic
GCCACATAACCGCGCTTATG+AGG 0.482912 3_3:-77929445 None:intergenic
GAGAAGCTACCAGAACATAA+AGG 0.487420 3_3:+77929864 Msa0474890:CDS
TCTCTGTTTACATCTACTTG+TGG 0.498460 3_3:+77930252 Msa0474890:three_prime_UTR
GTTAGTGGTTGAGAAATAGT+TGG 0.504921 3_3:+77930113 Msa0474890:exon
CTGAATTATCCCTTGTGAGA+AGG 0.505869 3_3:-77928407 None:intergenic
GCGGTTATGTGGCTGTTGTA+TGG 0.506943 3_3:+77929455 Msa0474890:CDS
TGAATTATCCCTTGTGAGAA+GGG 0.510719 3_3:-77928406 None:intergenic
TGGACAGCTCCCGAACGTTG+TGG 0.525233 3_3:+77929773 Msa0474890:intron
AAAACAGAAGAGAAGGTTAG+TGG 0.526127 3_3:+77930098 Msa0474890:CDS
TTACAGTAACATAGATATCT+CGG 0.536034 3_3:-77929487 None:intergenic
ATTGTGTTGATGAACTTTGT+GGG 0.543350 3_3:+77929117 Msa0474890:CDS
CGCATATAAAGGAGAAGAGA+CGG 0.552967 3_3:+77930203 Msa0474890:three_prime_UTR
GACGTATCAAGCTTCTTGGA+TGG 0.557609 3_3:+77929190 Msa0474890:CDS
CCTTCTCTCCCTTCTCACAA+GGG 0.557625 3_3:+77928398 Msa0474890:five_prime_UTR
TATCAACAAAACAGAAGAGA+AGG 0.559044 3_3:+77930091 Msa0474890:CDS
TTCCTAGCAAAACAAGAAGA+AGG 0.574175 3_3:+77929168 Msa0474890:CDS
TGTAAGAAGAAATCAACAGA+AGG 0.576661 3_3:+77928678 Msa0474890:CDS
GTGGTTGAGAAATAGTTGGC+GGG 0.578004 3_3:+77930117 Msa0474890:exon
GACAGCTCCCGAACGTTGTG+GGG 0.582179 3_3:+77929775 Msa0474890:intron
AGTGGTTGAGAAATAGTTGG+CGG 0.598698 3_3:+77930116 Msa0474890:exon
TTATGTAGATGTCAACATTG+AGG 0.602218 3_3:+77929091 Msa0474890:intron
GAAGTGAAAGTGGAAAAGGA+AGG 0.605323 3_3:-77929418 None:intergenic
ATGACTGTGGTGGGACGACA+TGG 0.619238 3_3:-77928475 None:intergenic
ACATAACCGCGCTTATGAGG+AGG 0.633283 3_3:-77929442 None:intergenic
TTACTGCCCCAAGAAAGTTA+GGG 0.638169 3_3:+77928848 Msa0474890:CDS
ACTTCTCCTCCTCATAAGCG+CGG 0.645264 3_3:+77929436 Msa0474890:CDS
CACGTACCCTAACTTTCTTG+GGG 0.660388 3_3:-77928854 None:intergenic
CGAAAGATAGATGACTGTGG+TGG 0.668857 3_3:-77928485 None:intergenic
ACTCACAATAATGCTTAAAG+GGG 0.675502 3_3:-77929247 None:intergenic
GAAAGATAGATGACTGTGGT+GGG 0.688303 3_3:-77928484 None:intergenic
AGTAACTCAAGACAAAACAA+AGG 0.690695 3_3:+77930043 Msa0474890:CDS
ATGCGAAAGATAGATGACTG+TGG 0.805804 3_3:-77928488 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTTACAATTAAATGAAG+TGG - chr3_3:77928590-77928609 None:intergenic 15.0%
!! AAAATAATTAATGTTGATCA+TGG - chr3_3:77929060-77929079 None:intergenic 15.0%
!! AAAATCAATACTACTAAATT+TGG + chr3_3:77929549-77929568 Msa0474890:intron 15.0%
!! CATTTGTATTAATTAATTTC+TGG + chr3_3:77929695-77929714 Msa0474890:intron 15.0%
!!! CAAAATCTTATGTTTTTTTA+TGG + chr3_3:77929753-77929772 Msa0474890:intron 15.0%
!! TTATGGAAATTATTATCTTA+TGG + chr3_3:77930372-77930391 Msa0474890:three_prime_UTR 15.0%
!! AAATTTACAATTAAATGAAG+TGG - chr3_3:77928590-77928609 None:intergenic 15.0%
!! AAAATAATTAATGTTGATCA+TGG - chr3_3:77929060-77929079 None:intergenic 15.0%
!! AAAATCAATACTACTAAATT+TGG + chr3_3:77929549-77929568 Msa0474890:intron 15.0%
!! CATTTGTATTAATTAATTTC+TGG + chr3_3:77929695-77929714 Msa0474890:intron 15.0%
!!! CAAAATCTTATGTTTTTTTA+TGG + chr3_3:77929753-77929772 Msa0474890:intron 15.0%
!! TTATGGAAATTATTATCTTA+TGG + chr3_3:77930372-77930391 Msa0474890:three_prime_UTR 15.0%
!!! GAAGGAAGATTTAATTTAAA+AGG - chr3_3:77928383-77928402 None:intergenic 20.0%
!! TTACAAATTCGATAAAATGT+TGG - chr3_3:77928663-77928682 None:intergenic 20.0%
!! GGGCATATATTATTATAATT+AGG - chr3_3:77930336-77930355 None:intergenic 20.0%
!!! GAAGGAAGATTTAATTTAAA+AGG - chr3_3:77928383-77928402 None:intergenic 20.0%
!! TTACAAATTCGATAAAATGT+TGG - chr3_3:77928663-77928682 None:intergenic 20.0%
!! GGGCATATATTATTATAATT+AGG - chr3_3:77930336-77930355 None:intergenic 20.0%
! ACAAAAATTAAGCTAGAGTT+TGG + chr3_3:77928353-77928372 Msa0474890:five_prime_UTR 25.0%
! ATTATTCTTTCTACTTACAG+TGG - chr3_3:77928558-77928577 None:intergenic 25.0%
!!! ATTTTTTTGACAAAGTTGCT+AGG + chr3_3:77928952-77928971 Msa0474890:intron 25.0%
! TTACAGTAACATAGATATCT+CGG - chr3_3:77929490-77929509 None:intergenic 25.0%
!!! TAGTTGCTATTTTGTCTTTA+TGG + chr3_3:77929718-77929737 Msa0474890:intron 25.0%
!!! ATATTTTGAGACGCATATAA+AGG + chr3_3:77930192-77930211 Msa0474890:three_prime_UTR 25.0%
!! ATAAACTTGTCTAGAATTGA+GGG - chr3_3:77930356-77930375 None:intergenic 25.0%
!! TTGATGTACTCTAAAGATTA+CGG + chr3_3:77930404-77930423 Msa0474890:three_prime_UTR 25.0%
! ACAAAAATTAAGCTAGAGTT+TGG + chr3_3:77928353-77928372 Msa0474890:five_prime_UTR 25.0%
! ATTATTCTTTCTACTTACAG+TGG - chr3_3:77928558-77928577 None:intergenic 25.0%
!!! ATTTTTTTGACAAAGTTGCT+AGG + chr3_3:77928952-77928971 Msa0474890:intron 25.0%
! TTACAGTAACATAGATATCT+CGG - chr3_3:77929490-77929509 None:intergenic 25.0%
!!! TAGTTGCTATTTTGTCTTTA+TGG + chr3_3:77929718-77929737 Msa0474890:intron 25.0%
!!! ATATTTTGAGACGCATATAA+AGG + chr3_3:77930192-77930211 Msa0474890:three_prime_UTR 25.0%
!! ATAAACTTGTCTAGAATTGA+GGG - chr3_3:77930356-77930375 None:intergenic 25.0%
!! TTGATGTACTCTAAAGATTA+CGG + chr3_3:77930404-77930423 Msa0474890:three_prime_UTR 25.0%
TGTAAGAAGAAATCAACAGA+AGG + chr3_3:77928678-77928697 Msa0474890:CDS 30.0%
TTTCCAATCAATTCCATATG+TGG + chr3_3:77928701-77928720 Msa0474890:CDS 30.0%
! TTTTATGCCTATACCAAGAA+GGG + chr3_3:77928753-77928772 Msa0474890:CDS 30.0%
TTTAACATTCCCACAATGTT+GGG - chr3_3:77928925-77928944 None:intergenic 30.0%
ATTTAACATTCCCACAATGT+TGG - chr3_3:77928926-77928945 None:intergenic 30.0%
TTATGTAGATGTCAACATTG+AGG + chr3_3:77929091-77929110 Msa0474890:intron 30.0%
! ATTGTGTTGATGAACTTTGT+GGG + chr3_3:77929117-77929136 Msa0474890:CDS 30.0%
ACTCACAATAATGCTTAAAG+GGG - chr3_3:77929250-77929269 None:intergenic 30.0%
CACTCACAATAATGCTTAAA+GGG - chr3_3:77929251-77929270 None:intergenic 30.0%
AAAAGCTAATTCTAGCATGT+CGG - chr3_3:77929290-77929309 None:intergenic 30.0%
AGGATGATTAAAATCATGCA+TGG - chr3_3:77929362-77929381 None:intergenic 30.0%
!!! TCATCATTTGTTGTTTTGCT+TGG - chr3_3:77929927-77929946 None:intergenic 30.0%
TATTGTGACTGTAGAGAAAA+AGG + chr3_3:77929971-77929990 Msa0474890:CDS 30.0%
!! TTGTTTTGTCTTGAGTTACT+TGG - chr3_3:77930043-77930062 None:intergenic 30.0%
AGTAACTCAAGACAAAACAA+AGG + chr3_3:77930043-77930062 Msa0474890:CDS 30.0%
TATCAACAAAACAGAAGAGA+AGG + chr3_3:77930091-77930110 Msa0474890:CDS 30.0%
! CATAAACTTGTCTAGAATTG+AGG - chr3_3:77930357-77930376 None:intergenic 30.0%
CTCAATTCTAGACAAGTTTA+TGG + chr3_3:77930355-77930374 Msa0474890:three_prime_UTR 30.0%
TGTAAGAAGAAATCAACAGA+AGG + chr3_3:77928678-77928697 Msa0474890:CDS 30.0%
TTTCCAATCAATTCCATATG+TGG + chr3_3:77928701-77928720 Msa0474890:CDS 30.0%
! TTTTATGCCTATACCAAGAA+GGG + chr3_3:77928753-77928772 Msa0474890:CDS 30.0%
TTTAACATTCCCACAATGTT+GGG - chr3_3:77928925-77928944 None:intergenic 30.0%
ATTTAACATTCCCACAATGT+TGG - chr3_3:77928926-77928945 None:intergenic 30.0%
TTATGTAGATGTCAACATTG+AGG + chr3_3:77929091-77929110 Msa0474890:intron 30.0%
! ATTGTGTTGATGAACTTTGT+GGG + chr3_3:77929117-77929136 Msa0474890:CDS 30.0%
ACTCACAATAATGCTTAAAG+GGG - chr3_3:77929250-77929269 None:intergenic 30.0%
CACTCACAATAATGCTTAAA+GGG - chr3_3:77929251-77929270 None:intergenic 30.0%
AAAAGCTAATTCTAGCATGT+CGG - chr3_3:77929290-77929309 None:intergenic 30.0%
AGGATGATTAAAATCATGCA+TGG - chr3_3:77929362-77929381 None:intergenic 30.0%
!!! TCATCATTTGTTGTTTTGCT+TGG - chr3_3:77929927-77929946 None:intergenic 30.0%
TATTGTGACTGTAGAGAAAA+AGG + chr3_3:77929971-77929990 Msa0474890:CDS 30.0%
!! TTGTTTTGTCTTGAGTTACT+TGG - chr3_3:77930043-77930062 None:intergenic 30.0%
AGTAACTCAAGACAAAACAA+AGG + chr3_3:77930043-77930062 Msa0474890:CDS 30.0%
TATCAACAAAACAGAAGAGA+AGG + chr3_3:77930091-77930110 Msa0474890:CDS 30.0%
! CATAAACTTGTCTAGAATTG+AGG - chr3_3:77930357-77930376 None:intergenic 30.0%
CTCAATTCTAGACAAGTTTA+TGG + chr3_3:77930355-77930374 Msa0474890:three_prime_UTR 30.0%
TGAATTATCCCTTGTGAGAA+GGG - chr3_3:77928409-77928428 None:intergenic 35.0%
AGTCATCTATCTTTCGCATT+TGG + chr3_3:77928492-77928511 Msa0474890:CDS 35.0%
CACTATTTAGTGCAATGCTT+TGG + chr3_3:77928727-77928746 Msa0474890:CDS 35.0%
! GTTTTATGCCTATACCAAGA+AGG + chr3_3:77928752-77928771 Msa0474890:CDS 35.0%
CTTATCACCATCAATGCATT+CGG + chr3_3:77928786-77928805 Msa0474890:CDS 35.0%
AGTAAGTGACAAAAACTGCA+AGG - chr3_3:77928833-77928852 None:intergenic 35.0%
! GATTGTGTTGATGAACTTTG+TGG + chr3_3:77929116-77929135 Msa0474890:CDS 35.0%
TTGATGAACTTTGTGGGATT+TGG + chr3_3:77929123-77929142 Msa0474890:CDS 35.0%
TTCCTAGCAAAACAAGAAGA+AGG + chr3_3:77929168-77929187 Msa0474890:CDS 35.0%
GCACTCACAATAATGCTTAA+AGG - chr3_3:77929252-77929271 None:intergenic 35.0%
TGGTAGCTTCTCATCAATTA+CGG - chr3_3:77929856-77929875 None:intergenic 35.0%
ACAATGTCTCCTTTATGTTC+TGG - chr3_3:77929876-77929895 None:intergenic 35.0%
TGATGAGAATGAGAAGAAAC+AGG + chr3_3:77930016-77930035 Msa0474890:CDS 35.0%
AAAACAGAAGAGAAGGTTAG+TGG + chr3_3:77930098-77930117 Msa0474890:CDS 35.0%
GTTAGTGGTTGAGAAATAGT+TGG + chr3_3:77930113-77930132 Msa0474890:exon 35.0%
TCTCTGTTTACATCTACTTG+TGG + chr3_3:77930252-77930271 Msa0474890:three_prime_UTR 35.0%
TGAATTATCCCTTGTGAGAA+GGG - chr3_3:77928409-77928428 None:intergenic 35.0%
AGTCATCTATCTTTCGCATT+TGG + chr3_3:77928492-77928511 Msa0474890:CDS 35.0%
CACTATTTAGTGCAATGCTT+TGG + chr3_3:77928727-77928746 Msa0474890:CDS 35.0%
! GTTTTATGCCTATACCAAGA+AGG + chr3_3:77928752-77928771 Msa0474890:CDS 35.0%
CTTATCACCATCAATGCATT+CGG + chr3_3:77928786-77928805 Msa0474890:CDS 35.0%
AGTAAGTGACAAAAACTGCA+AGG - chr3_3:77928833-77928852 None:intergenic 35.0%
! GATTGTGTTGATGAACTTTG+TGG + chr3_3:77929116-77929135 Msa0474890:CDS 35.0%
TTGATGAACTTTGTGGGATT+TGG + chr3_3:77929123-77929142 Msa0474890:CDS 35.0%
TTCCTAGCAAAACAAGAAGA+AGG + chr3_3:77929168-77929187 Msa0474890:CDS 35.0%
GCACTCACAATAATGCTTAA+AGG - chr3_3:77929252-77929271 None:intergenic 35.0%
TGGTAGCTTCTCATCAATTA+CGG - chr3_3:77929856-77929875 None:intergenic 35.0%
ACAATGTCTCCTTTATGTTC+TGG - chr3_3:77929876-77929895 None:intergenic 35.0%
TGATGAGAATGAGAAGAAAC+AGG + chr3_3:77930016-77930035 Msa0474890:CDS 35.0%
AAAACAGAAGAGAAGGTTAG+TGG + chr3_3:77930098-77930117 Msa0474890:CDS 35.0%
GTTAGTGGTTGAGAAATAGT+TGG + chr3_3:77930113-77930132 Msa0474890:exon 35.0%
TCTCTGTTTACATCTACTTG+TGG + chr3_3:77930252-77930271 Msa0474890:three_prime_UTR 35.0%
CTGAATTATCCCTTGTGAGA+AGG - chr3_3:77928410-77928429 None:intergenic 40.0%
GAAAGATAGATGACTGTGGT+GGG - chr3_3:77928487-77928506 None:intergenic 40.0%
ATGCGAAAGATAGATGACTG+TGG - chr3_3:77928491-77928510 None:intergenic 40.0%
! TCTTTCGCATTTGGAGTTCT+AGG + chr3_3:77928501-77928520 Msa0474890:CDS 40.0%
CAGCCACATATGGAATTGAT+TGG - chr3_3:77928707-77928726 None:intergenic 40.0%
CTAAATAGTGCAGCCACATA+TGG - chr3_3:77928717-77928736 None:intergenic 40.0%
AGTTTCACCCTTCTTGGTAT+AGG - chr3_3:77928763-77928782 None:intergenic 40.0%
AAGAAGAGTTTCACCCTTCT+TGG - chr3_3:77928769-77928788 None:intergenic 40.0%
TTACTGCCCCAAGAAAGTTA+GGG + chr3_3:77928848-77928867 Msa0474890:CDS 40.0%
ACACGTACCCTAACTTTCTT+GGG - chr3_3:77928858-77928877 None:intergenic 40.0%
TACACGTACCCTAACTTTCT+TGG - chr3_3:77928859-77928878 None:intergenic 40.0%
TAGCTAAAGTCCCAACATTG+TGG + chr3_3:77928912-77928931 Msa0474890:intron 40.0%
AGCTAAAGTCCCAACATTGT+GGG + chr3_3:77928913-77928932 Msa0474890:intron 40.0%
!!! GTCCTTCTTCTTGTTTTGCT+AGG - chr3_3:77929173-77929192 None:intergenic 40.0%
! AGGAAGGAACTCTACACTTT+TGG - chr3_3:77929405-77929424 None:intergenic 40.0%
GAAGTGAAAGTGGAAAAGGA+AGG - chr3_3:77929421-77929440 None:intergenic 40.0%
AGGAGAAGTGAAAGTGGAAA+AGG - chr3_3:77929425-77929444 None:intergenic 40.0%
AATGTTAACCCCACAACGTT+CGG - chr3_3:77929786-77929805 None:intergenic 40.0%
AACGTTGTGGGGTTAACATT+TGG + chr3_3:77929786-77929805 Msa0474890:CDS 40.0%
GGTAGCTTCTCATCAATTAC+GGG - chr3_3:77929855-77929874 None:intergenic 40.0%
GAGAAGCTACCAGAACATAA+AGG + chr3_3:77929864-77929883 Msa0474890:CDS 40.0%
AGTGGTTGAGAAATAGTTGG+CGG + chr3_3:77930116-77930135 Msa0474890:exon 40.0%
CGCATATAAAGGAGAAGAGA+CGG + chr3_3:77930203-77930222 Msa0474890:three_prime_UTR 40.0%
CTGAATTATCCCTTGTGAGA+AGG - chr3_3:77928410-77928429 None:intergenic 40.0%
GAAAGATAGATGACTGTGGT+GGG - chr3_3:77928487-77928506 None:intergenic 40.0%
ATGCGAAAGATAGATGACTG+TGG - chr3_3:77928491-77928510 None:intergenic 40.0%
! TCTTTCGCATTTGGAGTTCT+AGG + chr3_3:77928501-77928520 Msa0474890:CDS 40.0%
CAGCCACATATGGAATTGAT+TGG - chr3_3:77928707-77928726 None:intergenic 40.0%
CTAAATAGTGCAGCCACATA+TGG - chr3_3:77928717-77928736 None:intergenic 40.0%
AGTTTCACCCTTCTTGGTAT+AGG - chr3_3:77928763-77928782 None:intergenic 40.0%
AAGAAGAGTTTCACCCTTCT+TGG - chr3_3:77928769-77928788 None:intergenic 40.0%
TTACTGCCCCAAGAAAGTTA+GGG + chr3_3:77928848-77928867 Msa0474890:CDS 40.0%
ACACGTACCCTAACTTTCTT+GGG - chr3_3:77928858-77928877 None:intergenic 40.0%
TACACGTACCCTAACTTTCT+TGG - chr3_3:77928859-77928878 None:intergenic 40.0%
TAGCTAAAGTCCCAACATTG+TGG + chr3_3:77928912-77928931 Msa0474890:intron 40.0%
AGCTAAAGTCCCAACATTGT+GGG + chr3_3:77928913-77928932 Msa0474890:intron 40.0%
!!! GTCCTTCTTCTTGTTTTGCT+AGG - chr3_3:77929173-77929192 None:intergenic 40.0%
! AGGAAGGAACTCTACACTTT+TGG - chr3_3:77929405-77929424 None:intergenic 40.0%
GAAGTGAAAGTGGAAAAGGA+AGG - chr3_3:77929421-77929440 None:intergenic 40.0%
AGGAGAAGTGAAAGTGGAAA+AGG - chr3_3:77929425-77929444 None:intergenic 40.0%
AATGTTAACCCCACAACGTT+CGG - chr3_3:77929786-77929805 None:intergenic 40.0%
AACGTTGTGGGGTTAACATT+TGG + chr3_3:77929786-77929805 Msa0474890:CDS 40.0%
GGTAGCTTCTCATCAATTAC+GGG - chr3_3:77929855-77929874 None:intergenic 40.0%
GAGAAGCTACCAGAACATAA+AGG + chr3_3:77929864-77929883 Msa0474890:CDS 40.0%
AGTGGTTGAGAAATAGTTGG+CGG + chr3_3:77930116-77930135 Msa0474890:exon 40.0%
CGCATATAAAGGAGAAGAGA+CGG + chr3_3:77930203-77930222 Msa0474890:three_prime_UTR 40.0%
CGAAAGATAGATGACTGTGG+TGG - chr3_3:77928488-77928507 None:intergenic 45.0%
CACACAACCGAATGCATTGA+TGG - chr3_3:77928796-77928815 None:intergenic 45.0%
CTTACTGCCCCAAGAAAGTT+AGG + chr3_3:77928847-77928866 Msa0474890:CDS 45.0%
CACGTACCCTAACTTTCTTG+GGG - chr3_3:77928857-77928876 None:intergenic 45.0%
GAAGGACGTATCAAGCTTCT+TGG + chr3_3:77929186-77929205 Msa0474890:CDS 45.0%
GACGTATCAAGCTTCTTGGA+TGG + chr3_3:77929190-77929209 Msa0474890:CDS 45.0%
TGCGAATAACAACTCTCTGC+AGG - chr3_3:77929382-77929401 None:intergenic 45.0%
ATGAGGAGGAGAAGTGAAAG+TGG - chr3_3:77929431-77929450 None:intergenic 45.0%
ATGTTAACCCCACAACGTTC+GGG - chr3_3:77929785-77929804 None:intergenic 45.0%
GTGGTTGAGAAATAGTTGGC+GGG + chr3_3:77930117-77930136 Msa0474890:exon 45.0%
CGAAAGATAGATGACTGTGG+TGG - chr3_3:77928488-77928507 None:intergenic 45.0%
CACACAACCGAATGCATTGA+TGG - chr3_3:77928796-77928815 None:intergenic 45.0%
CTTACTGCCCCAAGAAAGTT+AGG + chr3_3:77928847-77928866 Msa0474890:CDS 45.0%
CACGTACCCTAACTTTCTTG+GGG - chr3_3:77928857-77928876 None:intergenic 45.0%
GAAGGACGTATCAAGCTTCT+TGG + chr3_3:77929186-77929205 Msa0474890:CDS 45.0%
GACGTATCAAGCTTCTTGGA+TGG + chr3_3:77929190-77929209 Msa0474890:CDS 45.0%
TGCGAATAACAACTCTCTGC+AGG - chr3_3:77929382-77929401 None:intergenic 45.0%
ATGAGGAGGAGAAGTGAAAG+TGG - chr3_3:77929431-77929450 None:intergenic 45.0%
ATGTTAACCCCACAACGTTC+GGG - chr3_3:77929785-77929804 None:intergenic 45.0%
GTGGTTGAGAAATAGTTGGC+GGG + chr3_3:77930117-77930136 Msa0474890:exon 45.0%
TCCTTCTCTCCCTTCTCACA+AGG + chr3_3:77928397-77928416 Msa0474890:five_prime_UTR 50.0%
CCTTCTCTCCCTTCTCACAA+GGG + chr3_3:77928398-77928417 Msa0474890:five_prime_UTR 50.0%
ACTTCTCCTCCTCATAAGCG+CGG + chr3_3:77929436-77929455 Msa0474890:CDS 50.0%
ACATAACCGCGCTTATGAGG+AGG - chr3_3:77929445-77929464 None:intergenic 50.0%
TCCTCATAAGCGCGGTTATG+TGG + chr3_3:77929444-77929463 Msa0474890:CDS 50.0%
GCGGTTATGTGGCTGTTGTA+TGG + chr3_3:77929455-77929474 Msa0474890:CDS 50.0%
TCCTTCTCTCCCTTCTCACA+AGG + chr3_3:77928397-77928416 Msa0474890:five_prime_UTR 50.0%
CCTTCTCTCCCTTCTCACAA+GGG + chr3_3:77928398-77928417 Msa0474890:five_prime_UTR 50.0%
ACTTCTCCTCCTCATAAGCG+CGG + chr3_3:77929436-77929455 Msa0474890:CDS 50.0%
ACATAACCGCGCTTATGAGG+AGG - chr3_3:77929445-77929464 None:intergenic 50.0%
TCCTCATAAGCGCGGTTATG+TGG + chr3_3:77929444-77929463 Msa0474890:CDS 50.0%
GCGGTTATGTGGCTGTTGTA+TGG + chr3_3:77929455-77929474 Msa0474890:CDS 50.0%
CCCTTGTGAGAAGGGAGAGA+AGG - chr3_3:77928401-77928420 None:intergenic 55.0%
! ATGACTGTGGTGGGACGACA+TGG - chr3_3:77928478-77928497 None:intergenic 55.0%
GCCACATAACCGCGCTTATG+AGG - chr3_3:77929448-77929467 None:intergenic 55.0%
CCCTTGTGAGAAGGGAGAGA+AGG - chr3_3:77928401-77928420 None:intergenic 55.0%
! ATGACTGTGGTGGGACGACA+TGG - chr3_3:77928478-77928497 None:intergenic 55.0%
GCCACATAACCGCGCTTATG+AGG - chr3_3:77929448-77929467 None:intergenic 55.0%
TGGACAGCTCCCGAACGTTG+TGG + chr3_3:77929773-77929792 Msa0474890:intron 60.0%
GGACAGCTCCCGAACGTTGT+GGG + chr3_3:77929774-77929793 Msa0474890:intron 60.0%
GACAGCTCCCGAACGTTGTG+GGG + chr3_3:77929775-77929794 Msa0474890:intron 60.0%
TGGACAGCTCCCGAACGTTG+TGG + chr3_3:77929773-77929792 Msa0474890:intron 60.0%
GGACAGCTCCCGAACGTTGT+GGG + chr3_3:77929774-77929793 Msa0474890:intron 60.0%
GACAGCTCCCGAACGTTGTG+GGG + chr3_3:77929775-77929794 Msa0474890:intron 60.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 77928336 77930450 77928336 ID=Msa0474890;Name=Msa0474890
chr3_3 mRNA 77928336 77930450 77928336 ID=Msa0474890-mRNA-1;Parent=Msa0474890;Name=Msa0474890-mRNA-1;_AED=0.19;_eAED=0.19;_QI=141|1|1|1|1|1|5|326|307
chr3_3 exon 77928336 77928559 77928336 ID=Msa0474890-mRNA-1:exon:20911;Parent=Msa0474890-mRNA-1
chr3_3 exon 77928659 77928869 77928659 ID=Msa0474890-mRNA-1:exon:20912;Parent=Msa0474890-mRNA-1
chr3_3 exon 77929099 77929263 77929099 ID=Msa0474890-mRNA-1:exon:20913;Parent=Msa0474890-mRNA-1
chr3_3 exon 77929386 77929505 77929386 ID=Msa0474890-mRNA-1:exon:20914;Parent=Msa0474890-mRNA-1
chr3_3 exon 77929780 77930450 77929780 ID=Msa0474890-mRNA-1:exon:20915;Parent=Msa0474890-mRNA-1
chr3_3 five_prime_UTR 77928336 77928476 77928336 ID=Msa0474890-mRNA-1:five_prime_utr;Parent=Msa0474890-mRNA-1
chr3_3 CDS 77928477 77928559 77928477 ID=Msa0474890-mRNA-1:cds;Parent=Msa0474890-mRNA-1
chr3_3 CDS 77928659 77928869 77928659 ID=Msa0474890-mRNA-1:cds;Parent=Msa0474890-mRNA-1
chr3_3 CDS 77929099 77929263 77929099 ID=Msa0474890-mRNA-1:cds;Parent=Msa0474890-mRNA-1
chr3_3 CDS 77929386 77929505 77929386 ID=Msa0474890-mRNA-1:cds;Parent=Msa0474890-mRNA-1
chr3_3 CDS 77929780 77930124 77929780 ID=Msa0474890-mRNA-1:cds;Parent=Msa0474890-mRNA-1
chr3_3 three_prime_UTR 77930125 77930450 77930125 ID=Msa0474890-mRNA-1:three_prime_utr;Parent=Msa0474890-mRNA-1
Gene Sequence

>Msa0474890

ATGTCGTCCCACCACAGTCATCTATCTTTCGCATTTGGAGTTCTAGGCAACATTACATCATTTCTTTGCTTTCTAGCACCACTACCAACATTTTATCGAATTTGTAAGAAGAAATCAACAGAAGGTTTCCAATCAATTCCATATGTGGCTGCACTATTTAGTGCAATGCTTTGGATGTTTTATGCCTATACCAAGAAGGGTGAAACTCTTCTTATCACCATCAATGCATTCGGTTGTGTGATAGAGACAATTTACCTTGCAGTTTTTGTCACTTACTGCCCCAAGAAAGTTAGGATGTCAACATTGAGGATGATTGTGTTGATGAACTTTGTGGGATTTGGCACGATTGTCTTGTTAACTCACTTCCTAGCAAAACAAGAAGAAGGACGTATCAAGCTTCTTGGATGGATTTGTGTAGTTTTTGCAACTAGTGTTTTTGCTGCCCCTTTAAGCATTATTAGAGTTGTTATTCGCACCAAAAGTGTAGAGTTCCTTCCTTTTCCACTTTCACTTCTCCTCCTCATAAGCGCGGTTATGTGGCTGTTGTATGGTCTTTCTCTCCGAGATATCTATGTTACTCTCCCGAACGTTGTGGGGTTAACATTTGGAATAGTTCAGATCACACTCTATGCAATGTACAGAAACAGCAAACCCGTAATTGATGAGAAGCTACCAGAACATAAAGGAGACATTGTCGATAAAGAAATTGAAAACGTAGTTGTTCCAAGCAAAACAACAAATGATGAGAAGAAACTAGAAGTGAATGTTGATATTGTGACTGTAGAGAAAAAGGAAGAGAAACAAGATGAAGAACATGATGAGAATGAGAAGAAACAGGACCAAGTAACTCAAGACAAAACAAAGGTTAAGAATGAAAATGACAACATTAATATCAACAAAACAGAAGAGAAGGTTAGTGGTTGA
Protein sequence

>Msa0474890

MSSHHSHLSFAFGVLGNITSFLCFLAPLPTFYRICKKKSTEGFQSIPYVAALFSAMLWMFYAYTKKGETLLITINAFGCVIETIYLAVFVTYCPKKVRMSTLRMIVLMNFVGFGTIVLLTHFLAKQEEGRIKLLGWICVVFATSVFAAPLSIIRVVIRTKSVEFLPFPLSLLLLISAVMWLLYGLSLRDIYVTLPNVVGLTFGIVQITLYAMYRNSKPVIDEKLPEHKGDIVDKEIENVVVPSKTTNDEKKLEVNVDIVTVEKKEEKQDEEHDENEKKQDQVTQDKTKVKNENDNININKTEEKVSG*