AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0428530

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0428530 MtrunA17_Chr3g0131951 96.104 308 11 1 1 307 63 370 0.0 582
Msa0428530 MtrunA17_Chr8g0386001 64.017 239 86 0 10 248 10 248 1.57e-114 332
Msa0428530 MtrunA17_Chr8g0386011 52.743 237 107 3 1 233 1 236 1.16e-85 257
Msa0428530 MtrunA17_Chr7g0214181 50.195 257 118 3 1 257 1 247 1.88e-81 247
Msa0428530 MtrunA17_Chr7g0214191 46.454 282 133 4 1 282 1 264 3.41e-81 246
Msa0428530 MtrunA17_Chr5g0428281 48.188 276 133 4 1 275 1 267 1.09e-80 245
Msa0428530 MtrunA17_Chr3g0131961 48.485 231 116 3 4 233 5 233 7.71e-76 233
Msa0428530 MtrunA17_Chr7g0215601 50.218 229 112 2 1 228 1 228 3.56e-71 220
Msa0428530 MtrunA17_Chr5g0444031 45.082 244 129 2 8 247 28 270 3.33e-68 213
Msa0428530 MtrunA17_Chr2g0295681 41.627 209 121 1 9 217 5 212 8.60e-50 165
Msa0428530 MtrunA17_Chr2g0278491 41.071 224 124 5 56 275 1 220 1.14e-47 159
Msa0428530 MtrunA17_Chr3g0119951 36.694 248 146 6 11 252 10 252 5.87e-44 150
Msa0428530 MtrunA17_Chr3g0125861 36.161 224 137 4 4 225 2 221 1.29e-41 144
Msa0428530 MtrunA17_Chr8g0388621 34.426 244 153 4 1 241 1 240 1.48e-41 144
Msa0428530 MtrunA17_Chr1g0158931 36.986 219 133 3 8 222 5 222 4.19e-41 143
Msa0428530 MtrunA17_Chr3g0119991 37.021 235 137 6 24 252 2 231 9.84e-41 142
Msa0428530 MtrunA17_Chr6g0450941 38.426 216 127 4 13 223 85 299 1.81e-40 143
Msa0428530 MtrunA17_Chr1g0159431 37.220 223 130 4 10 228 6 222 1.01e-39 139
Msa0428530 MtrunA17_Chr8g0359261 37.441 211 124 3 11 217 16 222 1.03e-36 131
Msa0428530 MtrunA17_Chr3g0126421 35.565 239 144 7 8 239 5 240 2.73e-36 130
Msa0428530 MtrunA17_Chr4g0059191 33.014 209 137 2 25 231 1 208 7.19e-36 128
Msa0428530 MtrunA17_Chr3g0126411 35.060 251 149 8 8 250 5 249 8.27e-36 129
Msa0428530 MtrunA17_Chr6g0450951 39.062 192 113 3 29 217 5 195 9.86e-35 125
Msa0428530 MtrunA17_Chr2g0314571 34.562 217 138 2 11 225 16 230 8.16e-34 124
Msa0428530 MtrunA17_Chr6g0450931 36.000 200 122 4 29 223 5 203 1.03e-32 120
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0428530 AT5G50800.1 50.512 293 137 5 1 288 1 290 2.01e-93 279
Msa0428530 AT5G23660.1 58.475 236 89 2 5 232 7 241 1.32e-92 276
Msa0428530 AT3G48740.1 51.246 281 123 5 9 281 11 285 1.82e-90 271
Msa0428530 AT4G25010.1 51.079 278 130 4 5 279 5 279 1.81e-89 268
Msa0428530 AT5G13170.1 45.695 302 149 6 1 302 4 290 1.10e-87 264
Msa0428530 AT5G50790.1 43.945 289 144 6 1 278 1 282 1.09e-80 246
Msa0428530 AT2G39060.1 42.972 249 122 2 8 237 8 255 5.67e-65 205
Msa0428530 AT4G15920.1 37.441 211 132 0 9 219 5 215 4.86e-52 171
Msa0428530 AT3G16690.3 37.668 223 137 2 2 223 33 254 8.34e-52 171
Msa0428530 AT3G16690.1 37.443 219 135 2 6 223 2 219 6.86e-51 168
Msa0428530 AT1G21460.1 40.637 251 130 6 12 254 8 247 2.93e-48 162
Msa0428530 AT4G10850.1 35.268 224 142 2 3 223 4 227 7.02e-44 151
Msa0428530 AT1G66770.1 32.489 237 158 1 13 247 14 250 7.49e-41 143
Msa0428530 AT5G40260.1 40.092 217 124 5 7 218 6 221 6.96e-40 140
Msa0428530 AT5G62850.1 33.790 219 139 4 1 216 1 216 3.34e-39 138
Msa0428530 AT5G53190.1 30.769 286 161 8 8 284 5 262 1.30e-38 137
Msa0428530 AT1G21460.2 43.784 185 100 3 12 194 8 190 1.30e-37 133
Msa0428530 AT3G28007.1 38.095 210 123 4 13 218 12 218 9.37e-35 127
Msa0428530 AT3G14770.1 35.945 217 131 4 13 226 18 229 1.38e-34 126
Msa0428530 AT5G40260.2 41.361 191 106 5 7 192 6 195 8.76e-34 123
Msa0428530 AT5G62850.2 34.973 183 114 3 13 192 12 192 1.69e-32 120
Msa0428530 AT3G16690.2 33.333 192 112 3 6 182 2 192 9.80e-28 107

Find 75 sgRNAs with CRISPR-Local

Find 202 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACATCATTTCTTTGCTTTC+TGG 0.098038 3_2:+79446830 Msa0428530:CDS
GAAGGAAGGTTTAATTTAAA+AGG 0.166644 3_2:-79446686 None:intergenic
AAGAAGAGTTTCACCCTTCT+TGG 0.240770 3_2:-79447066 None:intergenic
GCACTCACAATAATGCTTAA+AGG 0.256613 3_2:-79447555 None:intergenic
TACACGTACCCTAACTTTCT+TGG 0.259331 3_2:-79447156 None:intergenic
TTATGGAAATTATTATCTTA+TGG 0.274592 3_2:+79448674 Msa0428530:three_prime_UTR
CTTACTGCCCCAAGAAAGTT+AGG 0.280598 3_2:+79447147 Msa0428530:CDS
TATTGTGACTGTGGAGAAAA+AGG 0.285828 3_2:+79448272 Msa0428530:CDS
GGGCATATATTATTATAATT+AGG 0.291098 3_2:-79448634 None:intergenic
TACAACTTGTGGGTTAATAT+TGG 0.294196 3_2:-79448740 None:intergenic
TCTTTCGCCTTTGGAGTTCT+AGG 0.295855 3_2:+79446801 Msa0428530:CDS
ACACGTACCCTAACTTTCTT+GGG 0.302349 3_2:-79447155 None:intergenic
TTGATGAACTTTGTGGGATT+TGG 0.311697 3_2:+79447429 Msa0428530:CDS
AGTTTCACCCTTCTTGGTAT+AGG 0.322134 3_2:-79447060 None:intergenic
AACGTTGTGGGGTTAACATT+TGG 0.325987 3_2:+79448087 Msa0428530:CDS
AGGAAGGAACTCTACACTTT+TGG 0.334515 3_2:-79447708 None:intergenic
TGGTAGCTTCTCATCAATTA+CGG 0.338030 3_2:-79448154 None:intergenic
CACTCACAATAATGCTTAAA+GGG 0.349067 3_2:-79447554 None:intergenic
AGTCATCTATCTTTCGCCTT+TGG 0.350886 3_2:+79446792 Msa0428530:CDS
CACTATTTAGTGCAATGCTT+TGG 0.369787 3_2:+79447027 Msa0428530:CDS
CACACAACCGAATGCATTGA+TGG 0.370212 3_2:-79447093 None:intergenic
GGTAGCTTCTCATCAATTAC+GGG 0.374020 3_2:-79448153 None:intergenic
TTGATGTACTCTAAAGATTA+CGG 0.375925 3_2:+79448702 Msa0428530:three_prime_UTR
GAAGGACGTATCAAGCTTCT+TGG 0.381738 3_2:+79447492 Msa0428530:CDS
AATGTTAACCCCACAACGTT+CGG 0.388997 3_2:-79448084 None:intergenic
ACAATGTCTCCTTTATGTTC+TGG 0.402350 3_2:-79448174 None:intergenic
GGGTTTGCTGTTTCTGTACA+TGG 0.407593 3_2:-79448133 None:intergenic
CTAAATAGTGCAGCCACATA+TGG 0.415521 3_2:-79447014 None:intergenic
CTTATCACCATCAATGCATT+CGG 0.422646 3_2:+79447086 Msa0428530:CDS
AGGAGAAGTGAAAGTGGAAA+AGG 0.437747 3_2:-79447728 None:intergenic
ATGTTAACCCCACAACGTTC+GGG 0.444928 3_2:-79448083 None:intergenic
GGACAGCTCCCGAACGTTGT+GGG 0.447921 3_2:+79448075 Msa0428530:intron
TCCTTCTCTCCCTTCTCACA+AGG 0.448599 3_2:+79446703 Msa0428530:five_prime_UTR
CAGCCACATATGGAATTGAT+TGG 0.459983 3_2:-79447004 None:intergenic
TGATGAGAATGAGAAGAAAC+AGG 0.461226 3_2:+79448317 Msa0428530:CDS
TTACAAATTCGATAAAATGT+TGG 0.461818 3_2:-79446960 None:intergenic
TCCTCATAAGCGCGGTTATG+TGG 0.463599 3_2:+79447750 Msa0428530:CDS
GATTGTGTTGATGAACTTTG+TGG 0.471961 3_2:+79447422 Msa0428530:CDS
ATGAGGAGGAGAAGTGAAAG+TGG 0.474274 3_2:-79447734 None:intergenic
TTTCCAATCAATTCCATATG+TGG 0.478701 3_2:+79447001 Msa0428530:CDS
CCCTTGTGAGAAGGGAGAGA+AGG 0.480289 3_2:-79446704 None:intergenic
GCCACATAACCGCGCTTATG+AGG 0.482912 3_2:-79447751 None:intergenic
GAGAAGCTACCAGAACATAA+AGG 0.487420 3_2:+79448165 Msa0428530:CDS
TCTCTGTTTACATCTACTTG+TGG 0.498460 3_2:+79448553 Msa0428530:three_prime_UTR
GTTAGTGGTTGAGAAATAGT+TGG 0.504921 3_2:+79448414 Msa0428530:exon
CTGAATTATCCCTTGTGAGA+AGG 0.505869 3_2:-79446713 None:intergenic
GCGGTTATGTGGCTGTTGTA+TGG 0.506943 3_2:+79447761 Msa0428530:CDS
TGAATTATCCCTTGTGAGAA+GGG 0.510719 3_2:-79446712 None:intergenic
TGGACAGCTCCCGAACGTTG+TGG 0.525233 3_2:+79448074 Msa0428530:intron
AAAACAGAAGAGAAGGTTAG+TGG 0.526127 3_2:+79448399 Msa0428530:CDS
ATTGTGTTGATGAACTTTGT+GGG 0.543350 3_2:+79447423 Msa0428530:CDS
CGCATATAAAGGAGAAGAGA+CGG 0.552967 3_2:+79448504 Msa0428530:three_prime_UTR
GACGTATCAAGCTTCTTGGA+TGG 0.557609 3_2:+79447496 Msa0428530:CDS
CCTTCTCTCCCTTCTCACAA+GGG 0.557625 3_2:+79446704 Msa0428530:five_prime_UTR
TATCAACAAAACAGAAGAGA+AGG 0.559044 3_2:+79448392 Msa0428530:CDS
TGTGAGAAGGGAGAGAAGGA+AGG 0.572340 3_2:-79446700 None:intergenic
TTCCTAGCAAAACAAGAAGA+AGG 0.574175 3_2:+79447474 Msa0428530:CDS
TGTAAGAAGAAATCAACAGA+AGG 0.576661 3_2:+79446978 Msa0428530:CDS
GTGGTTGAGAAATAGTTGGC+GGG 0.578004 3_2:+79448418 Msa0428530:exon
GACAGCTCCCGAACGTTGTG+GGG 0.582179 3_2:+79448076 Msa0428530:intron
AGTGGTTGAGAAATAGTTGG+CGG 0.598698 3_2:+79448417 Msa0428530:exon
TTATGTAGATGTCAACATTG+AGG 0.602218 3_2:+79447397 Msa0428530:intron
GAAGTGAAAGTGGAAAAGGA+AGG 0.605323 3_2:-79447724 None:intergenic
GAAAGATAGATGACTGTGGT+GGG 0.607987 3_2:-79446784 None:intergenic
CGAAAGATAGATGACTGTGG+TGG 0.609394 3_2:-79446785 None:intergenic
ATGACTGTGGTGGGACGACA+TGG 0.619238 3_2:-79446775 None:intergenic
GAATGTTAATATTGTGACTG+TGG 0.633029 3_2:+79448263 Msa0428530:CDS
ACATAACCGCGCTTATGAGG+AGG 0.633283 3_2:-79447748 None:intergenic
TTACTGCCCCAAGAAAGTTA+GGG 0.638169 3_2:+79447148 Msa0428530:CDS
ACTTCTCCTCCTCATAAGCG+CGG 0.645264 3_2:+79447742 Msa0428530:CDS
AATGTTGCCTAGAACTCCAA+AGG 0.654062 3_2:-79446808 None:intergenic
CACGTACCCTAACTTTCTTG+GGG 0.660388 3_2:-79447154 None:intergenic
ACTCACAATAATGCTTAAAG+GGG 0.675502 3_2:-79447553 None:intergenic
AGTAACTCAAGACAAAACAA+AGG 0.690695 3_2:+79448344 Msa0428530:CDS
AGGCGAAAGATAGATGACTG+TGG 0.756668 3_2:-79446788 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTTACAATTAAATGAAG+TGG - chr3_2:79446890-79446909 None:intergenic 15.0%
!! AAAAAGATCAATACTAAATT+TGG + chr3_2:79447853-79447872 Msa0428530:intron 15.0%
!! TAAAAAAGTTTAAACAACAT+AGG - chr3_2:79447900-79447919 None:intergenic 15.0%
!!! CAAAATCTTATGTTTTTTTA+TGG + chr3_2:79448054-79448073 Msa0428530:intron 15.0%
!! TTATGGAAATTATTATCTTA+TGG + chr3_2:79448674-79448693 Msa0428530:three_prime_UTR 15.0%
!! AAATTTACAATTAAATGAAG+TGG - chr3_2:79446890-79446909 None:intergenic 15.0%
!! AAAAAGATCAATACTAAATT+TGG + chr3_2:79447853-79447872 Msa0428530:intron 15.0%
!! TAAAAAAGTTTAAACAACAT+AGG - chr3_2:79447900-79447919 None:intergenic 15.0%
!!! CAAAATCTTATGTTTTTTTA+TGG + chr3_2:79448054-79448073 Msa0428530:intron 15.0%
!! TTATGGAAATTATTATCTTA+TGG + chr3_2:79448674-79448693 Msa0428530:three_prime_UTR 15.0%
!! TTACAAATTCGATAAAATGT+TGG - chr3_2:79446963-79446982 None:intergenic 20.0%
!! AAAATAATTCATGTTGATCA+TGG - chr3_2:79447366-79447385 None:intergenic 20.0%
!!! TTTGCGTTTTTATTAATTTC+TGG + chr3_2:79447996-79448015 Msa0428530:intron 20.0%
!! GGGCATATATTATTATAATT+AGG - chr3_2:79448637-79448656 None:intergenic 20.0%
!!! TAAACTTTTTCTAGAATTGA+GGG - chr3_2:79448657-79448676 None:intergenic 20.0%
!!! ATAAACTTTTTCTAGAATTG+AGG - chr3_2:79448658-79448677 None:intergenic 20.0%
!!! TCAATTCTAGAAAAAGTTTA+TGG + chr3_2:79448657-79448676 Msa0428530:three_prime_UTR 20.0%
!! TTACAAATTCGATAAAATGT+TGG - chr3_2:79446963-79446982 None:intergenic 20.0%
!! AAAATAATTCATGTTGATCA+TGG - chr3_2:79447366-79447385 None:intergenic 20.0%
!!! TTTGCGTTTTTATTAATTTC+TGG + chr3_2:79447996-79448015 Msa0428530:intron 20.0%
!! GGGCATATATTATTATAATT+AGG - chr3_2:79448637-79448656 None:intergenic 20.0%
!!! TAAACTTTTTCTAGAATTGA+GGG - chr3_2:79448657-79448676 None:intergenic 20.0%
!!! ATAAACTTTTTCTAGAATTG+AGG - chr3_2:79448658-79448677 None:intergenic 20.0%
!!! TCAATTCTAGAAAAAGTTTA+TGG + chr3_2:79448657-79448676 Msa0428530:three_prime_UTR 20.0%
! ACAAAAATTAAGCTAGAGTT+TGG + chr3_2:79446659-79446678 Msa0428530:five_prime_UTR 25.0%
!! GAAGGAAGGTTTAATTTAAA+AGG - chr3_2:79446689-79446708 None:intergenic 25.0%
! ATTATTCTTTCTACTTACAG+TGG - chr3_2:79446858-79446877 None:intergenic 25.0%
! TTTAACATTCCAACAATGTT+GGG - chr3_2:79447225-79447244 None:intergenic 25.0%
! ATTTAACATTCCAACAATGT+TGG - chr3_2:79447226-79447245 None:intergenic 25.0%
!!! ATTTTTTGACAAAAGTTGCT+AGG + chr3_2:79447258-79447277 Msa0428530:intron 25.0%
!!! TAGTTGCTATTTTGTCTTAA+TGG + chr3_2:79448019-79448038 Msa0428530:intron 25.0%
!!! ATATTTTGAGACGCATATAA+AGG + chr3_2:79448493-79448512 Msa0428530:three_prime_UTR 25.0%
!! TTGATGTACTCTAAAGATTA+CGG + chr3_2:79448702-79448721 Msa0428530:three_prime_UTR 25.0%
! ACAAAAATTAAGCTAGAGTT+TGG + chr3_2:79446659-79446678 Msa0428530:five_prime_UTR 25.0%
!! GAAGGAAGGTTTAATTTAAA+AGG - chr3_2:79446689-79446708 None:intergenic 25.0%
! ATTATTCTTTCTACTTACAG+TGG - chr3_2:79446858-79446877 None:intergenic 25.0%
! TTTAACATTCCAACAATGTT+GGG - chr3_2:79447225-79447244 None:intergenic 25.0%
! ATTTAACATTCCAACAATGT+TGG - chr3_2:79447226-79447245 None:intergenic 25.0%
!!! ATTTTTTGACAAAAGTTGCT+AGG + chr3_2:79447258-79447277 Msa0428530:intron 25.0%
!!! TAGTTGCTATTTTGTCTTAA+TGG + chr3_2:79448019-79448038 Msa0428530:intron 25.0%
!!! ATATTTTGAGACGCATATAA+AGG + chr3_2:79448493-79448512 Msa0428530:three_prime_UTR 25.0%
!! TTGATGTACTCTAAAGATTA+CGG + chr3_2:79448702-79448721 Msa0428530:three_prime_UTR 25.0%
TACATCATTTCTTTGCTTTC+TGG + chr3_2:79446830-79446849 Msa0428530:CDS 30.0%
TGTAAGAAGAAATCAACAGA+AGG + chr3_2:79446978-79446997 Msa0428530:CDS 30.0%
TTTCCAATCAATTCCATATG+TGG + chr3_2:79447001-79447020 Msa0428530:CDS 30.0%
! TTTTATGCCTATACCAAGAA+GGG + chr3_2:79447053-79447072 Msa0428530:CDS 30.0%
TTATGTAGATGTCAACATTG+AGG + chr3_2:79447397-79447416 Msa0428530:intron 30.0%
! ATTGTGTTGATGAACTTTGT+GGG + chr3_2:79447423-79447442 Msa0428530:CDS 30.0%
ACTCACAATAATGCTTAAAG+GGG - chr3_2:79447556-79447575 None:intergenic 30.0%
CACTCACAATAATGCTTAAA+GGG - chr3_2:79447557-79447576 None:intergenic 30.0%
AAAAGCTAATTCTAGCATGT+CGG - chr3_2:79447596-79447615 None:intergenic 30.0%
ATAAACTAATCATCCATGCA+TGG + chr3_2:79447652-79447671 Msa0428530:intron 30.0%
AAGATGATTAAAACCATGCA+TGG - chr3_2:79447668-79447687 None:intergenic 30.0%
!!! TCATCATTTGTTGTTTTGCT+TGG - chr3_2:79448228-79448247 None:intergenic 30.0%
GAATGTTAATATTGTGACTG+TGG + chr3_2:79448263-79448282 Msa0428530:CDS 30.0%
!! TTGTTTTGTCTTGAGTTACT+TGG - chr3_2:79448344-79448363 None:intergenic 30.0%
AGTAACTCAAGACAAAACAA+AGG + chr3_2:79448344-79448363 Msa0428530:CDS 30.0%
TATCAACAAAACAGAAGAGA+AGG + chr3_2:79448392-79448411 Msa0428530:CDS 30.0%
TACATCATTTCTTTGCTTTC+TGG + chr3_2:79446830-79446849 Msa0428530:CDS 30.0%
TGTAAGAAGAAATCAACAGA+AGG + chr3_2:79446978-79446997 Msa0428530:CDS 30.0%
TTTCCAATCAATTCCATATG+TGG + chr3_2:79447001-79447020 Msa0428530:CDS 30.0%
! TTTTATGCCTATACCAAGAA+GGG + chr3_2:79447053-79447072 Msa0428530:CDS 30.0%
TTATGTAGATGTCAACATTG+AGG + chr3_2:79447397-79447416 Msa0428530:intron 30.0%
! ATTGTGTTGATGAACTTTGT+GGG + chr3_2:79447423-79447442 Msa0428530:CDS 30.0%
ACTCACAATAATGCTTAAAG+GGG - chr3_2:79447556-79447575 None:intergenic 30.0%
CACTCACAATAATGCTTAAA+GGG - chr3_2:79447557-79447576 None:intergenic 30.0%
AAAAGCTAATTCTAGCATGT+CGG - chr3_2:79447596-79447615 None:intergenic 30.0%
ATAAACTAATCATCCATGCA+TGG + chr3_2:79447652-79447671 Msa0428530:intron 30.0%
AAGATGATTAAAACCATGCA+TGG - chr3_2:79447668-79447687 None:intergenic 30.0%
!!! TCATCATTTGTTGTTTTGCT+TGG - chr3_2:79448228-79448247 None:intergenic 30.0%
GAATGTTAATATTGTGACTG+TGG + chr3_2:79448263-79448282 Msa0428530:CDS 30.0%
!! TTGTTTTGTCTTGAGTTACT+TGG - chr3_2:79448344-79448363 None:intergenic 30.0%
AGTAACTCAAGACAAAACAA+AGG + chr3_2:79448344-79448363 Msa0428530:CDS 30.0%
TATCAACAAAACAGAAGAGA+AGG + chr3_2:79448392-79448411 Msa0428530:CDS 30.0%
TGAATTATCCCTTGTGAGAA+GGG - chr3_2:79446715-79446734 None:intergenic 35.0%
CACTATTTAGTGCAATGCTT+TGG + chr3_2:79447027-79447046 Msa0428530:CDS 35.0%
! GTTTTATGCCTATACCAAGA+AGG + chr3_2:79447052-79447071 Msa0428530:CDS 35.0%
CTTATCACCATCAATGCATT+CGG + chr3_2:79447086-79447105 Msa0428530:CDS 35.0%
! GATTGTGTTGATGAACTTTG+TGG + chr3_2:79447422-79447441 Msa0428530:CDS 35.0%
TTGATGAACTTTGTGGGATT+TGG + chr3_2:79447429-79447448 Msa0428530:CDS 35.0%
TTCCTAGCAAAACAAGAAGA+AGG + chr3_2:79447474-79447493 Msa0428530:CDS 35.0%
GCACTCACAATAATGCTTAA+AGG - chr3_2:79447558-79447577 None:intergenic 35.0%
TGGTAGCTTCTCATCAATTA+CGG - chr3_2:79448157-79448176 None:intergenic 35.0%
ACAATGTCTCCTTTATGTTC+TGG - chr3_2:79448177-79448196 None:intergenic 35.0%
TATTGTGACTGTGGAGAAAA+AGG + chr3_2:79448272-79448291 Msa0428530:CDS 35.0%
TGATGAGAATGAGAAGAAAC+AGG + chr3_2:79448317-79448336 Msa0428530:CDS 35.0%
AAAACAGAAGAGAAGGTTAG+TGG + chr3_2:79448399-79448418 Msa0428530:CDS 35.0%
GTTAGTGGTTGAGAAATAGT+TGG + chr3_2:79448414-79448433 Msa0428530:exon 35.0%
TCTCTGTTTACATCTACTTG+TGG + chr3_2:79448553-79448572 Msa0428530:three_prime_UTR 35.0%
TGAATTATCCCTTGTGAGAA+GGG - chr3_2:79446715-79446734 None:intergenic 35.0%
CACTATTTAGTGCAATGCTT+TGG + chr3_2:79447027-79447046 Msa0428530:CDS 35.0%
! GTTTTATGCCTATACCAAGA+AGG + chr3_2:79447052-79447071 Msa0428530:CDS 35.0%
CTTATCACCATCAATGCATT+CGG + chr3_2:79447086-79447105 Msa0428530:CDS 35.0%
! GATTGTGTTGATGAACTTTG+TGG + chr3_2:79447422-79447441 Msa0428530:CDS 35.0%
TTGATGAACTTTGTGGGATT+TGG + chr3_2:79447429-79447448 Msa0428530:CDS 35.0%
TTCCTAGCAAAACAAGAAGA+AGG + chr3_2:79447474-79447493 Msa0428530:CDS 35.0%
GCACTCACAATAATGCTTAA+AGG - chr3_2:79447558-79447577 None:intergenic 35.0%
TGGTAGCTTCTCATCAATTA+CGG - chr3_2:79448157-79448176 None:intergenic 35.0%
ACAATGTCTCCTTTATGTTC+TGG - chr3_2:79448177-79448196 None:intergenic 35.0%
TATTGTGACTGTGGAGAAAA+AGG + chr3_2:79448272-79448291 Msa0428530:CDS 35.0%
TGATGAGAATGAGAAGAAAC+AGG + chr3_2:79448317-79448336 Msa0428530:CDS 35.0%
AAAACAGAAGAGAAGGTTAG+TGG + chr3_2:79448399-79448418 Msa0428530:CDS 35.0%
GTTAGTGGTTGAGAAATAGT+TGG + chr3_2:79448414-79448433 Msa0428530:exon 35.0%
TCTCTGTTTACATCTACTTG+TGG + chr3_2:79448553-79448572 Msa0428530:three_prime_UTR 35.0%
CTGAATTATCCCTTGTGAGA+AGG - chr3_2:79446716-79446735 None:intergenic 40.0%
GAAAGATAGATGACTGTGGT+GGG - chr3_2:79446787-79446806 None:intergenic 40.0%
AGTCATCTATCTTTCGCCTT+TGG + chr3_2:79446792-79446811 Msa0428530:CDS 40.0%
AATGTTGCCTAGAACTCCAA+AGG - chr3_2:79446811-79446830 None:intergenic 40.0%
CAGCCACATATGGAATTGAT+TGG - chr3_2:79447007-79447026 None:intergenic 40.0%
CTAAATAGTGCAGCCACATA+TGG - chr3_2:79447017-79447036 None:intergenic 40.0%
AGTTTCACCCTTCTTGGTAT+AGG - chr3_2:79447063-79447082 None:intergenic 40.0%
AAGAAGAGTTTCACCCTTCT+TGG - chr3_2:79447069-79447088 None:intergenic 40.0%
AGTAAGTGAGAAAAACTGCG+AGG - chr3_2:79447133-79447152 None:intergenic 40.0%
TTACTGCCCCAAGAAAGTTA+GGG + chr3_2:79447148-79447167 Msa0428530:CDS 40.0%
ACACGTACCCTAACTTTCTT+GGG - chr3_2:79447158-79447177 None:intergenic 40.0%
TACACGTACCCTAACTTTCT+TGG - chr3_2:79447159-79447178 None:intergenic 40.0%
AGCTAAAGTCCCAACATTGT+TGG + chr3_2:79447213-79447232 Msa0428530:intron 40.0%
!!! GTCCTTCTTCTTGTTTTGCT+AGG - chr3_2:79447479-79447498 None:intergenic 40.0%
! AGGAAGGAACTCTACACTTT+TGG - chr3_2:79447711-79447730 None:intergenic 40.0%
GAAGTGAAAGTGGAAAAGGA+AGG - chr3_2:79447727-79447746 None:intergenic 40.0%
AGGAGAAGTGAAAGTGGAAA+AGG - chr3_2:79447731-79447750 None:intergenic 40.0%
AATGTTAACCCCACAACGTT+CGG - chr3_2:79448087-79448106 None:intergenic 40.0%
AACGTTGTGGGGTTAACATT+TGG + chr3_2:79448087-79448106 Msa0428530:CDS 40.0%
GGTAGCTTCTCATCAATTAC+GGG - chr3_2:79448156-79448175 None:intergenic 40.0%
GAGAAGCTACCAGAACATAA+AGG + chr3_2:79448165-79448184 Msa0428530:CDS 40.0%
AGTGGTTGAGAAATAGTTGG+CGG + chr3_2:79448417-79448436 Msa0428530:exon 40.0%
CGCATATAAAGGAGAAGAGA+CGG + chr3_2:79448504-79448523 Msa0428530:three_prime_UTR 40.0%
CTGAATTATCCCTTGTGAGA+AGG - chr3_2:79446716-79446735 None:intergenic 40.0%
GAAAGATAGATGACTGTGGT+GGG - chr3_2:79446787-79446806 None:intergenic 40.0%
AGTCATCTATCTTTCGCCTT+TGG + chr3_2:79446792-79446811 Msa0428530:CDS 40.0%
AATGTTGCCTAGAACTCCAA+AGG - chr3_2:79446811-79446830 None:intergenic 40.0%
CAGCCACATATGGAATTGAT+TGG - chr3_2:79447007-79447026 None:intergenic 40.0%
CTAAATAGTGCAGCCACATA+TGG - chr3_2:79447017-79447036 None:intergenic 40.0%
AGTTTCACCCTTCTTGGTAT+AGG - chr3_2:79447063-79447082 None:intergenic 40.0%
AAGAAGAGTTTCACCCTTCT+TGG - chr3_2:79447069-79447088 None:intergenic 40.0%
AGTAAGTGAGAAAAACTGCG+AGG - chr3_2:79447133-79447152 None:intergenic 40.0%
TTACTGCCCCAAGAAAGTTA+GGG + chr3_2:79447148-79447167 Msa0428530:CDS 40.0%
ACACGTACCCTAACTTTCTT+GGG - chr3_2:79447158-79447177 None:intergenic 40.0%
TACACGTACCCTAACTTTCT+TGG - chr3_2:79447159-79447178 None:intergenic 40.0%
AGCTAAAGTCCCAACATTGT+TGG + chr3_2:79447213-79447232 Msa0428530:intron 40.0%
!!! GTCCTTCTTCTTGTTTTGCT+AGG - chr3_2:79447479-79447498 None:intergenic 40.0%
! AGGAAGGAACTCTACACTTT+TGG - chr3_2:79447711-79447730 None:intergenic 40.0%
GAAGTGAAAGTGGAAAAGGA+AGG - chr3_2:79447727-79447746 None:intergenic 40.0%
AGGAGAAGTGAAAGTGGAAA+AGG - chr3_2:79447731-79447750 None:intergenic 40.0%
AATGTTAACCCCACAACGTT+CGG - chr3_2:79448087-79448106 None:intergenic 40.0%
AACGTTGTGGGGTTAACATT+TGG + chr3_2:79448087-79448106 Msa0428530:CDS 40.0%
GGTAGCTTCTCATCAATTAC+GGG - chr3_2:79448156-79448175 None:intergenic 40.0%
GAGAAGCTACCAGAACATAA+AGG + chr3_2:79448165-79448184 Msa0428530:CDS 40.0%
AGTGGTTGAGAAATAGTTGG+CGG + chr3_2:79448417-79448436 Msa0428530:exon 40.0%
CGCATATAAAGGAGAAGAGA+CGG + chr3_2:79448504-79448523 Msa0428530:three_prime_UTR 40.0%
CGAAAGATAGATGACTGTGG+TGG - chr3_2:79446788-79446807 None:intergenic 45.0%
AGGCGAAAGATAGATGACTG+TGG - chr3_2:79446791-79446810 None:intergenic 45.0%
!! TCTTTCGCCTTTGGAGTTCT+AGG + chr3_2:79446801-79446820 Msa0428530:CDS 45.0%
CACACAACCGAATGCATTGA+TGG - chr3_2:79447096-79447115 None:intergenic 45.0%
CTTACTGCCCCAAGAAAGTT+AGG + chr3_2:79447147-79447166 Msa0428530:CDS 45.0%
CACGTACCCTAACTTTCTTG+GGG - chr3_2:79447157-79447176 None:intergenic 45.0%
GAAGGACGTATCAAGCTTCT+TGG + chr3_2:79447492-79447511 Msa0428530:CDS 45.0%
GACGTATCAAGCTTCTTGGA+TGG + chr3_2:79447496-79447515 Msa0428530:CDS 45.0%
ATGAGGAGGAGAAGTGAAAG+TGG - chr3_2:79447737-79447756 None:intergenic 45.0%
ATGTTAACCCCACAACGTTC+GGG - chr3_2:79448086-79448105 None:intergenic 45.0%
!! GGGTTTGCTGTTTCTGTACA+TGG - chr3_2:79448136-79448155 None:intergenic 45.0%
GTGGTTGAGAAATAGTTGGC+GGG + chr3_2:79448418-79448437 Msa0428530:exon 45.0%
CGAAAGATAGATGACTGTGG+TGG - chr3_2:79446788-79446807 None:intergenic 45.0%
AGGCGAAAGATAGATGACTG+TGG - chr3_2:79446791-79446810 None:intergenic 45.0%
!! TCTTTCGCCTTTGGAGTTCT+AGG + chr3_2:79446801-79446820 Msa0428530:CDS 45.0%
CACACAACCGAATGCATTGA+TGG - chr3_2:79447096-79447115 None:intergenic 45.0%
CTTACTGCCCCAAGAAAGTT+AGG + chr3_2:79447147-79447166 Msa0428530:CDS 45.0%
CACGTACCCTAACTTTCTTG+GGG - chr3_2:79447157-79447176 None:intergenic 45.0%
GAAGGACGTATCAAGCTTCT+TGG + chr3_2:79447492-79447511 Msa0428530:CDS 45.0%
GACGTATCAAGCTTCTTGGA+TGG + chr3_2:79447496-79447515 Msa0428530:CDS 45.0%
ATGAGGAGGAGAAGTGAAAG+TGG - chr3_2:79447737-79447756 None:intergenic 45.0%
ATGTTAACCCCACAACGTTC+GGG - chr3_2:79448086-79448105 None:intergenic 45.0%
!! GGGTTTGCTGTTTCTGTACA+TGG - chr3_2:79448136-79448155 None:intergenic 45.0%
GTGGTTGAGAAATAGTTGGC+GGG + chr3_2:79448418-79448437 Msa0428530:exon 45.0%
!!! CTTTTTAATTATTATTTTTA+TGG + chr3_2:79447611-79447630 Msa0428530:intron 5.0%
!!! CTTTTTAATTATTATTTTTA+TGG + chr3_2:79447611-79447630 Msa0428530:intron 5.0%
TGTGAGAAGGGAGAGAAGGA+AGG - chr3_2:79446703-79446722 None:intergenic 50.0%
TCCTTCTCTCCCTTCTCACA+AGG + chr3_2:79446703-79446722 Msa0428530:five_prime_UTR 50.0%
CCTTCTCTCCCTTCTCACAA+GGG + chr3_2:79446704-79446723 Msa0428530:five_prime_UTR 50.0%
ACTTCTCCTCCTCATAAGCG+CGG + chr3_2:79447742-79447761 Msa0428530:CDS 50.0%
ACATAACCGCGCTTATGAGG+AGG - chr3_2:79447751-79447770 None:intergenic 50.0%
TCCTCATAAGCGCGGTTATG+TGG + chr3_2:79447750-79447769 Msa0428530:CDS 50.0%
GCGGTTATGTGGCTGTTGTA+TGG + chr3_2:79447761-79447780 Msa0428530:CDS 50.0%
TGTGAGAAGGGAGAGAAGGA+AGG - chr3_2:79446703-79446722 None:intergenic 50.0%
TCCTTCTCTCCCTTCTCACA+AGG + chr3_2:79446703-79446722 Msa0428530:five_prime_UTR 50.0%
CCTTCTCTCCCTTCTCACAA+GGG + chr3_2:79446704-79446723 Msa0428530:five_prime_UTR 50.0%
ACTTCTCCTCCTCATAAGCG+CGG + chr3_2:79447742-79447761 Msa0428530:CDS 50.0%
ACATAACCGCGCTTATGAGG+AGG - chr3_2:79447751-79447770 None:intergenic 50.0%
TCCTCATAAGCGCGGTTATG+TGG + chr3_2:79447750-79447769 Msa0428530:CDS 50.0%
GCGGTTATGTGGCTGTTGTA+TGG + chr3_2:79447761-79447780 Msa0428530:CDS 50.0%
CCCTTGTGAGAAGGGAGAGA+AGG - chr3_2:79446707-79446726 None:intergenic 55.0%
! ATGACTGTGGTGGGACGACA+TGG - chr3_2:79446778-79446797 None:intergenic 55.0%
GCCACATAACCGCGCTTATG+AGG - chr3_2:79447754-79447773 None:intergenic 55.0%
CCCTTGTGAGAAGGGAGAGA+AGG - chr3_2:79446707-79446726 None:intergenic 55.0%
! ATGACTGTGGTGGGACGACA+TGG - chr3_2:79446778-79446797 None:intergenic 55.0%
GCCACATAACCGCGCTTATG+AGG - chr3_2:79447754-79447773 None:intergenic 55.0%
TGGACAGCTCCCGAACGTTG+TGG + chr3_2:79448074-79448093 Msa0428530:intron 60.0%
GGACAGCTCCCGAACGTTGT+GGG + chr3_2:79448075-79448094 Msa0428530:intron 60.0%
GACAGCTCCCGAACGTTGTG+GGG + chr3_2:79448076-79448095 Msa0428530:intron 60.0%
TGGACAGCTCCCGAACGTTG+TGG + chr3_2:79448074-79448093 Msa0428530:intron 60.0%
GGACAGCTCCCGAACGTTGT+GGG + chr3_2:79448075-79448094 Msa0428530:intron 60.0%
GACAGCTCCCGAACGTTGTG+GGG + chr3_2:79448076-79448095 Msa0428530:intron 60.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 79446642 79448748 79446642 ID=Msa0428530;Name=Msa0428530
chr3_2 mRNA 79446642 79448748 79446642 ID=Msa0428530-mRNA-1;Parent=Msa0428530;Name=Msa0428530-mRNA-1;_AED=0.04;_eAED=0.04;_QI=135|1|1|1|1|1|5|323|307
chr3_2 exon 79446642 79446859 79446642 ID=Msa0428530-mRNA-1:exon:21416;Parent=Msa0428530-mRNA-1
chr3_2 exon 79446959 79447169 79446959 ID=Msa0428530-mRNA-1:exon:21417;Parent=Msa0428530-mRNA-1
chr3_2 exon 79447405 79447569 79447405 ID=Msa0428530-mRNA-1:exon:21418;Parent=Msa0428530-mRNA-1
chr3_2 exon 79447692 79447811 79447692 ID=Msa0428530-mRNA-1:exon:21419;Parent=Msa0428530-mRNA-1
chr3_2 exon 79448081 79448748 79448081 ID=Msa0428530-mRNA-1:exon:21420;Parent=Msa0428530-mRNA-1
chr3_2 five_prime_UTR 79446642 79446776 79446642 ID=Msa0428530-mRNA-1:five_prime_utr;Parent=Msa0428530-mRNA-1
chr3_2 CDS 79446777 79446859 79446777 ID=Msa0428530-mRNA-1:cds;Parent=Msa0428530-mRNA-1
chr3_2 CDS 79446959 79447169 79446959 ID=Msa0428530-mRNA-1:cds;Parent=Msa0428530-mRNA-1
chr3_2 CDS 79447405 79447569 79447405 ID=Msa0428530-mRNA-1:cds;Parent=Msa0428530-mRNA-1
chr3_2 CDS 79447692 79447811 79447692 ID=Msa0428530-mRNA-1:cds;Parent=Msa0428530-mRNA-1
chr3_2 CDS 79448081 79448425 79448081 ID=Msa0428530-mRNA-1:cds;Parent=Msa0428530-mRNA-1
chr3_2 three_prime_UTR 79448426 79448748 79448426 ID=Msa0428530-mRNA-1:three_prime_utr;Parent=Msa0428530-mRNA-1
Gene Sequence

>Msa0428530

ATGTCGTCCCACCACAGTCATCTATCTTTCGCCTTTGGAGTTCTAGGCAACATTACATCATTTCTTTGCTTTCTGGCACCACTACCAACATTTTATCGAATTTGTAAGAAGAAATCAACAGAAGGTTTCCAATCAATTCCATATGTGGCTGCACTATTTAGTGCAATGCTTTGGATGTTTTATGCCTATACCAAGAAGGGTGAAACTCTTCTTATCACCATCAATGCATTCGGTTGTGTGATAGAGACAATTTACCTCGCAGTTTTTCTCACTTACTGCCCCAAGAAAGTTAGGATGTCAACATTGAGGATGATTGTGTTGATGAACTTTGTGGGATTTGGCACGATTGTCTTGTTAACTCACTTCCTAGCAAAACAAGAAGAAGGACGTATCAAGCTTCTTGGATGGATTTGTGTAGTTTTTGCAACTAGTGTTTTTGCTGCCCCTTTAAGCATTATTAGAGTTGTTATTCGCACCAAAAGTGTAGAGTTCCTTCCTTTTCCACTTTCACTTCTCCTCCTCATAAGCGCGGTTATGTGGCTGTTGTATGGTCTTTCTCTCAGAGATATCTATGTCACTCTCCCGAACGTTGTGGGGTTAACATTTGGAATAGTTCAGATCACACTATATGCCATGTACAGAAACAGCAAACCCGTAATTGATGAGAAGCTACCAGAACATAAAGGAGACATTGTCGACAAAAAAATTGAAAACGTAGTTGTTCCAAGCAAAACAACAAATGATGAGAAGAAACTAGAAGTGAATGTTAATATTGTGACTGTGGAGAAAAAGGAAGAGAAACAAGATGAAGAACATGATGAGAATGAGAAGAAACAGGACCAAGTAACTCAAGACAAAACAAAGGTTAAGAATGAAAATGACAACATTAATATCAACAAAACAGAAGAGAAGGTTAGTGGTTGA
Protein sequence

>Msa0428530

MSSHHSHLSFAFGVLGNITSFLCFLAPLPTFYRICKKKSTEGFQSIPYVAALFSAMLWMFYAYTKKGETLLITINAFGCVIETIYLAVFLTYCPKKVRMSTLRMIVLMNFVGFGTIVLLTHFLAKQEEGRIKLLGWICVVFATSVFAAPLSIIRVVIRTKSVEFLPFPLSLLLLISAVMWLLYGLSLRDIYVTLPNVVGLTFGIVQITLYAMYRNSKPVIDEKLPEHKGDIVDKKIENVVVPSKTTNDEKKLEVNVNIVTVEKKEEKQDEEHDENEKKQDQVTQDKTKVKNENDNININKTEEKVSG*