AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0481400

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0481400 MtrunA17_Chr8g0349741 98.462 195 3 0 17 211 1 195 1.22e-140 394
Msa0481400 MtrunA17_Chr7g0247011 66.667 195 64 1 17 211 1 194 2.09e-87 259
Msa0481400 MtrunA17_Chr7g0247041 60.914 197 69 3 23 211 6 202 7.88e-77 233
Msa0481400 MtrunA17_Chr8g0349851 59.113 203 69 3 22 211 6 207 4.50e-76 231
Msa0481400 MtrunA17_Chr8g0349811 57.971 207 74 3 17 211 1 206 6.39e-76 230
Msa0481400 MtrunA17_Chr8g0349781 58.454 207 73 3 17 211 1 206 2.23e-75 229
Msa0481400 MtrunA17_Chr8g0349761 61.194 201 65 4 23 211 8 207 5.92e-75 228
Msa0481400 MtrunA17_Chr7g0247031 61.979 192 69 3 22 211 7 196 3.74e-74 226
Msa0481400 MtrunA17_Chr8g0349801 56.522 207 77 3 17 211 1 206 5.26e-73 223
Msa0481400 MtrunA17_Chr8g0349701 56.931 202 79 4 17 211 1 201 1.16e-72 222
Msa0481400 MtrunA17_Chr8g0349721 56.436 202 80 4 17 211 1 201 2.39e-72 221
Msa0481400 MtrunA17_Chr8g0349771 56.039 207 78 3 17 211 1 206 4.94e-72 220
Msa0481400 MtrunA17_Chr8g0349751 55.450 211 77 3 15 211 49 256 1.58e-71 221
Msa0481400 MtrunA17_Chr8g0349671 56.585 205 78 3 17 211 1 204 7.35e-70 215
Msa0481400 MtrunA17_Chr8g0349831 56.650 203 74 3 22 211 7 208 1.98e-69 214
Msa0481400 MtrunA17_Chr8g0349641 55.610 205 80 3 17 211 1 204 5.02e-67 207
Msa0481400 MtrunA17_Chr5g0425391 51.220 205 89 4 17 211 1 204 2.25e-63 198
Msa0481400 MtrunA17_Chr7g0267881 51.295 193 73 2 23 211 9 184 1.16e-60 189
Msa0481400 MtrunA17_Chr3g0118511 51.208 207 80 6 23 211 3 206 1.78e-52 171
Msa0481400 MtrunA17_Chr8g0349651 52.632 171 70 4 51 211 14 183 2.19e-49 162
Msa0481400 MtrunA17_Chr3g0131521 43.878 196 88 5 20 209 32 211 2.14e-37 132
Msa0481400 MtrunA17_Chr1g0160511 41.905 210 98 6 7 209 24 216 8.67e-34 122
Msa0481400 MtrunA17_Chr8g0385581 38.384 198 97 6 20 208 34 215 1.26e-26 103
Msa0481400 MtrunA17_Chr4g0045711 47.826 92 44 2 20 107 19 110 1.60e-15 70.1
Msa0481400 MtrunA17_Chr4g0058731 32.143 112 73 3 14 124 40 149 2.73e-11 62.0
Msa0481400 MtrunA17_Chr7g0270461 36.957 92 53 1 23 114 49 135 4.34e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0481400 AT3G61220.1 57.143 203 79 3 17 211 1 203 4.08e-74 226
Msa0481400 AT3G61220.2 57.868 197 75 3 23 211 14 210 3.21e-73 224
Msa0481400 AT3G61220.3 57.868 197 75 3 23 211 38 234 1.00e-72 223
Msa0481400 AT2G24190.1 53.202 203 87 3 17 211 1 203 8.44e-71 218
Msa0481400 AT2G24190.2 53.807 197 83 3 23 211 12 208 4.64e-70 216
Msa0481400 AT1G01800.1 53.695 203 84 3 19 211 1 203 1.82e-68 211
Msa0481400 AT5G61830.1 43.077 195 91 5 20 208 34 214 1.77e-34 125
Msa0481400 AT5G61830.2 43.077 195 91 5 20 208 34 214 1.77e-34 125
Msa0481400 AT5G51030.1 38.776 196 98 6 20 209 35 214 2.79e-33 121
Msa0481400 AT3G59710.3 43.299 194 90 6 20 210 16 192 5.99e-33 120
Msa0481400 AT3G59710.1 43.299 194 90 6 20 210 26 202 8.38e-33 120
Msa0481400 AT3G59710.2 43.299 194 90 6 20 210 45 221 2.23e-32 119
Msa0481400 AT1G01800.2 50.877 114 47 2 107 211 55 168 2.69e-30 112
Msa0481400 AT5G50700.1 31.788 151 91 3 26 176 51 189 3.69e-14 70.5
Msa0481400 AT5G50600.1 31.788 151 91 3 26 176 51 189 3.69e-14 70.5

Find 56 sgRNAs with CRISPR-Local

Find 143 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTACTGCAGGACTATTAA+AGG 0.190820 3_3:+87467872 Msa0481400:CDS
TTCAGATATGCTGTTGTTAC+AGG 0.201198 3_3:+87466563 Msa0481400:intron
AGTTCTCTCCAATTAATAAT+TGG 0.222426 3_3:-87467726 None:intergenic
TCGAGTGATTCCACCACTTC+TGG 0.273816 3_3:+87469007 Msa0481400:three_prime_UTR
ACCAATGAAGCAATACTAAA+AGG 0.302446 3_3:-87466755 None:intergenic
AGGTTGAACCAATTATTAAT+TGG 0.308846 3_3:+87467718 Msa0481400:intron
GGTATATTATCTGTTGAAAA+TGG 0.316053 3_3:+87468953 Msa0481400:three_prime_UTR
TAAAATTAATCATCTATCTA+TGG 0.342933 3_3:+87466437 Msa0481400:CDS
ACGCAATTAATACATAAGTT+TGG 0.345471 3_3:-87468890 None:intergenic
ATGTATTAATTGCGTTTGTC+CGG 0.349424 3_3:+87468898 Msa0481400:three_prime_UTR
GTTTGGAAGACTTGATATCT+TGG 0.353617 3_3:+87466796 Msa0481400:CDS
ATTGGAAACTGTTAAAAGAT+TGG 0.358632 3_3:+87466604 Msa0481400:CDS
TTTAATTGAATCTAAATGAA+TGG 0.373999 3_3:-87466418 None:intergenic
ACAGGATCAAATAAAGGTAT+AGG 0.380633 3_3:+87466581 Msa0481400:CDS
TGGTTTCAACACAGACGATA+TGG 0.383538 3_3:+87467598 Msa0481400:CDS
AATAAACAGCAATGAACAAA+TGG 0.385084 3_3:-87469136 None:intergenic
GTTAAAAGATTGGCTTCAAA+TGG 0.389901 3_3:+87466614 Msa0481400:CDS
TGCATAATAACAAATTACTA+TGG 0.411597 3_3:+87467777 Msa0481400:CDS
GTAAATAATGCAGGAATCAA+TGG 0.443701 3_3:+87467578 Msa0481400:CDS
GTTAATGTTTCTTCTACTGC+AGG 0.448645 3_3:+87467861 Msa0481400:CDS
GAGTTTGTTAAGACTCAGTT+TGG 0.448827 3_3:+87466779 Msa0481400:CDS
ATCAAATAAAGGTATAGGAT+TGG 0.463443 3_3:+87466586 Msa0481400:CDS
ATATGATAGGATATGTGTCT+TGG 0.480820 3_3:+87469095 Msa0481400:three_prime_UTR
GTTGTTACAGGATCAAATAA+AGG 0.492642 3_3:+87466575 Msa0481400:CDS
TGTATTAATTGCGTTTGTCC+GGG 0.494685 3_3:+87468899 Msa0481400:three_prime_UTR
CTATGGCAATTTCAAAAGAA+AGG 0.498850 3_3:+87466454 Msa0481400:CDS
TACCTCGTCTATTAGCTCTT+CGG 0.500938 3_3:-87468738 None:intergenic
TTATTAATCAAATGAAATGT+GGG 0.504860 3_3:+87469154 Msa0481400:three_prime_UTR
TTCAGTACATATCAAACGAA+TGG 0.506130 3_3:+87468681 Msa0481400:intron
TTTATTAATCAAATGAAATG+TGG 0.512170 3_3:+87469153 Msa0481400:three_prime_UTR
CATGTGCAGGTAAATAATGC+AGG 0.516920 3_3:+87467569 Msa0481400:intron
ACAGCTAGAAATCAGAAAAG+AGG 0.518413 3_3:+87466653 Msa0481400:CDS
ACATATCAAACGAATGGGCT+AGG 0.522049 3_3:+87468687 Msa0481400:CDS
GAATCAGATAACTGAAGAAG+AGG 0.526731 3_3:-87467828 None:intergenic
TCAGTACATATCAAACGAAT+GGG 0.528974 3_3:+87468682 Msa0481400:intron
GAAGAAACATTAACAATCAC+AGG 0.532332 3_3:-87467852 None:intergenic
TTATAGGCACAAAGATATGT+TGG 0.548863 3_3:-87468815 None:intergenic
ACAAGGTTCACTTGAAAACA+AGG 0.553399 3_3:+87468787 Msa0481400:exon
ATAAACAGCAATGAACAAAT+GGG 0.555758 3_3:-87469135 None:intergenic
CAGCTAGAAATCAGAAAAGA+GGG 0.559811 3_3:+87466654 Msa0481400:CDS
GCTAATAGACGAGGTACTGA+AGG 0.563423 3_3:+87468745 Msa0481400:CDS
TTTCAATGTATTAAAATGGG+GGG 0.578258 3_3:+87469072 Msa0481400:three_prime_UTR
GATAGGATATGTGTCTTGGA+TGG 0.587395 3_3:+87469099 Msa0481400:three_prime_UTR
ACTGAGTCAAACTTATGAGA+TGG 0.603605 3_3:+87467746 Msa0481400:CDS
AGCGAAGAAGTGTCCAGAAG+TGG 0.605903 3_3:-87469020 None:intergenic
GTTCACTTGAAAACAAGGGT+TGG 0.606906 3_3:+87468792 Msa0481400:three_prime_UTR
TATTAATCAAATGAAATGTG+GGG 0.615096 3_3:+87469155 Msa0481400:three_prime_UTR
ATAAGCTAGAAGCCTTGTGT+AGG 0.622776 3_3:-87468861 None:intergenic
ATAATAACAAATTACTATGG+TGG 0.628363 3_3:+87467780 Msa0481400:CDS
TTTCTGAAAGACTTCTAACA+AGG 0.629519 3_3:+87468770 Msa0481400:exon
CAGCTGTGAATTCCTACACA+AGG 0.629636 3_3:+87468849 Msa0481400:three_prime_UTR
CAAGGTTCACTTGAAAACAA+GGG 0.637160 3_3:+87468788 Msa0481400:three_prime_UTR
ACAGATATGAATAGAAACAC+AGG 0.645826 3_3:+87468932 Msa0481400:three_prime_UTR
GAAGAAGTGTCCAGAAGTGG+TGG 0.653264 3_3:-87469017 None:intergenic
ATCCGAAGAGCTAATAGACG+AGG 0.706099 3_3:+87468736 Msa0481400:CDS
TGGTGGTAAAGAAACTACTG+AGG 0.726056 3_3:+87467797 Msa0481400:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATATTTTTTTAGTTATCT+TGG + chr3_3:87467023-87467042 Msa0481400:intron 10.0%
!! TTTAATTGAATCTAAATGAA+TGG - chr3_3:87466421-87466440 None:intergenic 15.0%
!! TAAAATTAATCATCTATCTA+TGG + chr3_3:87466437-87466456 Msa0481400:CDS 15.0%
!! CTTTGATAAAAGAAATTAAA+GGG - chr3_3:87466520-87466539 None:intergenic 15.0%
!!! ATGTTTTTCATTAGTTAATT+TGG - chr3_3:87468483-87468502 None:intergenic 15.0%
!! TTTATTAATCAAATGAAATG+TGG + chr3_3:87469153-87469172 Msa0481400:three_prime_UTR 15.0%
!! TTATTAATCAAATGAAATGT+GGG + chr3_3:87469154-87469173 Msa0481400:three_prime_UTR 15.0%
!!! AATTTCTACTAAATTTTAGT+TGG + chr3_3:87469197-87469216 Msa0481400:three_prime_UTR 15.0%
!! GCTTTGATAAAAGAAATTAA+AGG - chr3_3:87466521-87466540 None:intergenic 20.0%
!! ATTCTGAATTACCATTTATT+TGG + chr3_3:87466935-87466954 Msa0481400:intron 20.0%
!! TTCTTGATTCTTAATTATCT+AGG + chr3_3:87466996-87467015 Msa0481400:intron 20.0%
!! CTAAGTTATTTATCAAAACA+AGG - chr3_3:87467086-87467105 None:intergenic 20.0%
!!! ATTATCACAAATTTTAGTCA+TGG - chr3_3:87467512-87467531 None:intergenic 20.0%
!! AAAATTCAAGGCTATTATTA+TGG - chr3_3:87467549-87467568 None:intergenic 20.0%
!!! TTTCTTTGTTATGAATATTC+AGG + chr3_3:87467698-87467717 Msa0481400:intron 20.0%
!! TGCATAATAACAAATTACTA+TGG + chr3_3:87467777-87467796 Msa0481400:CDS 20.0%
!! ATAATAACAAATTACTATGG+TGG + chr3_3:87467780-87467799 Msa0481400:CDS 20.0%
!!! CAATATTTTTCGTTTTTTAG+AGG - chr3_3:87467983-87468002 None:intergenic 20.0%
!! CTCAAAAAATTATCCTATAA+AGG + chr3_3:87468062-87468081 Msa0481400:intron 20.0%
!!! AATTGGTACTTTTCATTTTT+CGG + chr3_3:87468121-87468140 Msa0481400:intron 20.0%
!! AAAAAATGATGAACTCTATA+TGG - chr3_3:87468150-87468169 None:intergenic 20.0%
!! TGAATTTCTCAAATTATTAG+TGG - chr3_3:87468247-87468266 None:intergenic 20.0%
!!! AGGTTTTCAATGTATTAAAA+TGG + chr3_3:87469068-87469087 Msa0481400:three_prime_UTR 20.0%
!!! GGTTTTCAATGTATTAAAAT+GGG + chr3_3:87469069-87469088 Msa0481400:three_prime_UTR 20.0%
!!! GTTTTCAATGTATTAAAATG+GGG + chr3_3:87469070-87469089 Msa0481400:three_prime_UTR 20.0%
!!! TTTTCAATGTATTAAAATGG+GGG + chr3_3:87469071-87469090 Msa0481400:three_prime_UTR 20.0%
!! TATTAATCAAATGAAATGTG+GGG + chr3_3:87469155-87469174 Msa0481400:three_prime_UTR 20.0%
!! AGGTACCTTTTATGTTTATT+TGG + chr3_3:87466474-87466493 Msa0481400:intron 25.0%
! TTGGTCCAAATAAACATAAA+AGG - chr3_3:87466482-87466501 None:intergenic 25.0%
!! AGGGATCTTTTAACATTTAT+TGG - chr3_3:87466501-87466520 None:intergenic 25.0%
!! ATCAAATAAAGGTATAGGAT+TGG + chr3_3:87466586-87466605 Msa0481400:CDS 25.0%
! ATTGGAAACTGTTAAAAGAT+TGG + chr3_3:87466604-87466623 Msa0481400:CDS 25.0%
!!! AGAAAATTGGCTTTACATTA+AGG - chr3_3:87466835-87466854 None:intergenic 25.0%
!! TTAATGTAAAGCCAATTTTC+TGG + chr3_3:87466834-87466853 Msa0481400:intron 25.0%
! ATCATAATGATCCAGAAAAT+TGG - chr3_3:87466848-87466867 None:intergenic 25.0%
! TTTATCCAAGTGTTCAAAAA+TGG - chr3_3:87467162-87467181 None:intergenic 25.0%
! ACTGTTTGAAAGAACTTATA+GGG - chr3_3:87467279-87467298 None:intergenic 25.0%
! ACAAACTTTGGATAAACTAA+TGG + chr3_3:87467464-87467483 Msa0481400:intron 25.0%
!! TTGTTTTCTTTAAACTGTCT+GGG + chr3_3:87467630-87467649 Msa0481400:intron 25.0%
! AGGTTGAACCAATTATTAAT+TGG + chr3_3:87467718-87467737 Msa0481400:intron 25.0%
! AGTTCTCTCCAATTAATAAT+TGG - chr3_3:87467729-87467748 None:intergenic 25.0%
!! AAGTCTTTGAATTTTTCTGA+AGG - chr3_3:87467953-87467972 None:intergenic 25.0%
!!! ATTGGTACTTTTCATTTTTC+GGG + chr3_3:87468122-87468141 Msa0481400:intron 25.0%
! TTCAGTGTAATTATATGTGT+TGG + chr3_3:87468263-87468282 Msa0481400:intron 25.0%
! ACTAACATAAGACAGATATA+AGG - chr3_3:87468417-87468436 None:intergenic 25.0%
! TTGATGTGTATTTCATTAGT+CGG - chr3_3:87468456-87468475 None:intergenic 25.0%
! ACTAATGAAATACACATCAA+AGG + chr3_3:87468456-87468475 Msa0481400:intron 25.0%
! ACAGTTTATTTATGTTGTTC+AGG - chr3_3:87468644-87468663 None:intergenic 25.0%
! ACGCAATTAATACATAAGTT+TGG - chr3_3:87468893-87468912 None:intergenic 25.0%
!!! ATATCTGTTTTAACAAAACC+CGG - chr3_3:87468920-87468939 None:intergenic 25.0%
! GGTATATTATCTGTTGAAAA+TGG + chr3_3:87468953-87468972 Msa0481400:three_prime_UTR 25.0%
! TTTCAATGTATTAAAATGGG+GGG + chr3_3:87469072-87469091 Msa0481400:three_prime_UTR 25.0%
! ATAAACAGCAATGAACAAAT+GGG - chr3_3:87469138-87469157 None:intergenic 25.0%
! AATAAACAGCAATGAACAAA+TGG - chr3_3:87469139-87469158 None:intergenic 25.0%
CTATGGCAATTTCAAAAGAA+AGG + chr3_3:87466454-87466473 Msa0481400:CDS 30.0%
GTTGTTACAGGATCAAATAA+AGG + chr3_3:87466575-87466594 Msa0481400:CDS 30.0%
! ACAGGATCAAATAAAGGTAT+AGG + chr3_3:87466581-87466600 Msa0481400:CDS 30.0%
!! GTTAAAAGATTGGCTTCAAA+TGG + chr3_3:87466614-87466633 Msa0481400:CDS 30.0%
! TCCTTTTAGTATTGCTTCAT+TGG + chr3_3:87466754-87466773 Msa0481400:CDS 30.0%
ACCAATGAAGCAATACTAAA+AGG - chr3_3:87466758-87466777 None:intergenic 30.0%
! TACCATTTATTTGGTGAATC+TGG + chr3_3:87466944-87466963 Msa0481400:intron 30.0%
TTCTTCATTCTCATTCTCAT+AGG + chr3_3:87467060-87467079 Msa0481400:intron 30.0%
TGATAAAAGCTCTTAGCAAA+AGG - chr3_3:87467115-87467134 None:intergenic 30.0%
!!! ATAAGCCATTTTTGAACACT+TGG + chr3_3:87467154-87467173 Msa0481400:intron 30.0%
GACTGTTTGAAAGAACTTAT+AGG - chr3_3:87467280-87467299 None:intergenic 30.0%
!!! ACTTATTTTAGCTGATCTAC+TGG - chr3_3:87467318-87467337 None:intergenic 30.0%
GTTTATCCAAAGTTTGTAAC+AGG - chr3_3:87467461-87467480 None:intergenic 30.0%
GTAAATAATGCAGGAATCAA+TGG + chr3_3:87467578-87467597 Msa0481400:CDS 30.0%
!! CTTGTTTTCTTTAAACTGTC+TGG + chr3_3:87467629-87467648 Msa0481400:intron 30.0%
AATAGCCTTTGATTAAGCTT+TGG + chr3_3:87467675-87467694 Msa0481400:intron 30.0%
AGAAACCAAAGCTTAATCAA+AGG - chr3_3:87467683-87467702 None:intergenic 30.0%
GAAGAAACATTAACAATCAC+AGG - chr3_3:87467855-87467874 None:intergenic 30.0%
CTATCAGAGATTATTTAGAC+TGG - chr3_3:87468030-87468049 None:intergenic 30.0%
!! TTGGTACTTTTCATTTTTCG+GGG + chr3_3:87468123-87468142 Msa0481400:intron 30.0%
TCATAGGTGAAAACACATTT+CGG - chr3_3:87468179-87468198 None:intergenic 30.0%
TCTCAAATTATTAGTGGTGT+TGG - chr3_3:87468241-87468260 None:intergenic 30.0%
! GTAATTATATGTGTTGGTGT+CGG + chr3_3:87468269-87468288 Msa0481400:intron 30.0%
ACATACAAGAAAAACATTCG+AGG + chr3_3:87468520-87468539 Msa0481400:intron 30.0%
!! CGAGGATGTTTTTATAAGTT+TGG + chr3_3:87468538-87468557 Msa0481400:intron 30.0%
GGTTAATTCAGAGTCTATAA+CGG + chr3_3:87468559-87468578 Msa0481400:intron 30.0%
CTGATGTAAAGTGGTAATTT+CGG - chr3_3:87468611-87468630 None:intergenic 30.0%
ACATTGTAACTGATGTAAAG+TGG - chr3_3:87468620-87468639 None:intergenic 30.0%
TTCAGTACATATCAAACGAA+TGG + chr3_3:87468681-87468700 Msa0481400:intron 30.0%
TCAGTACATATCAAACGAAT+GGG + chr3_3:87468682-87468701 Msa0481400:intron 30.0%
! TTTCTGAAAGACTTCTAACA+AGG + chr3_3:87468770-87468789 Msa0481400:exon 30.0%
TTATAGGCACAAAGATATGT+TGG - chr3_3:87468818-87468837 None:intergenic 30.0%
ATGTATTAATTGCGTTTGTC+CGG + chr3_3:87468898-87468917 Msa0481400:three_prime_UTR 30.0%
ACAGATATGAATAGAAACAC+AGG + chr3_3:87468932-87468951 Msa0481400:three_prime_UTR 30.0%
!! AGATTTGTCTTGCTTTTGAA+AGG + chr3_3:87469048-87469067 Msa0481400:three_prime_UTR 30.0%
ATATGATAGGATATGTGTCT+TGG + chr3_3:87469095-87469114 Msa0481400:three_prime_UTR 30.0%
TTCAGATATGCTGTTGTTAC+AGG + chr3_3:87466563-87466582 Msa0481400:intron 35.0%
ACAGCTAGAAATCAGAAAAG+AGG + chr3_3:87466653-87466672 Msa0481400:CDS 35.0%
CAGCTAGAAATCAGAAAAGA+GGG + chr3_3:87466654-87466673 Msa0481400:CDS 35.0%
!! GAGTTTGTTAAGACTCAGTT+TGG + chr3_3:87466779-87466798 Msa0481400:CDS 35.0%
GTTTGGAAGACTTGATATCT+TGG + chr3_3:87466796-87466815 Msa0481400:CDS 35.0%
CACCAGATTCACCAAATAAA+TGG - chr3_3:87466949-87466968 None:intergenic 35.0%
! TTATTTGGTGAATCTGGTGA+AGG + chr3_3:87466950-87466969 Msa0481400:intron 35.0%
GCACAAACACTTCAGATTAA+AGG + chr3_3:87467411-87467430 Msa0481400:intron 35.0%
TCCAAAGTTTGTAACAGGTT+GGG - chr3_3:87467456-87467475 None:intergenic 35.0%
ATCCAAAGTTTGTAACAGGT+TGG - chr3_3:87467457-87467476 None:intergenic 35.0%
TACCTGCACATGAAAATTCA+AGG - chr3_3:87467561-87467580 None:intergenic 35.0%
! TAAACTGTCTGGGATGATAA+TGG + chr3_3:87467640-87467659 Msa0481400:intron 35.0%
ACTGAGTCAAACTTATGAGA+TGG + chr3_3:87467746-87467765 Msa0481400:CDS 35.0%
GAATCAGATAACTGAAGAAG+AGG - chr3_3:87467831-87467850 None:intergenic 35.0%
GTTAATGTTTCTTCTACTGC+AGG + chr3_3:87467861-87467880 Msa0481400:CDS 35.0%
TTCTACTGCAGGACTATTAA+AGG + chr3_3:87467872-87467891 Msa0481400:CDS 35.0%
CAGGCAAATTAGTCCTTTAT+AGG - chr3_3:87468078-87468097 None:intergenic 35.0%
CCTCAAAAGTCTCAAATATC+AGG - chr3_3:87468097-87468116 None:intergenic 35.0%
!! CCTGATATTTGAGACTTTTG+AGG + chr3_3:87468094-87468113 Msa0481400:intron 35.0%
!! GAGACTTTTGAGGACTAAAT+TGG + chr3_3:87468104-87468123 Msa0481400:intron 35.0%
ACAAGGTTCACTTGAAAACA+AGG + chr3_3:87468787-87468806 Msa0481400:exon 35.0%
CAAGGTTCACTTGAAAACAA+GGG + chr3_3:87468788-87468807 Msa0481400:three_prime_UTR 35.0%
!!! AGCTGCTTTTGAGAGTTTAT+AGG - chr3_3:87468834-87468853 None:intergenic 35.0%
TGTATTAATTGCGTTTGTCC+GGG + chr3_3:87468899-87468918 Msa0481400:three_prime_UTR 35.0%
TTAAAATGGGGGGATATGAT+AGG + chr3_3:87469082-87469101 Msa0481400:three_prime_UTR 35.0%
! CTTCAGATTAAAGGCGTGTT+TGG + chr3_3:87467420-87467439 Msa0481400:intron 40.0%
! TTTGTAACAGGTTGGGACAA+CGG - chr3_3:87467449-87467468 None:intergenic 40.0%
TCCCAACCTGTTACAAACTT+TGG + chr3_3:87467452-87467471 Msa0481400:intron 40.0%
! AGCCTTGAATTTTCATGTGC+AGG + chr3_3:87467556-87467575 Msa0481400:intron 40.0%
CATGTGCAGGTAAATAATGC+AGG + chr3_3:87467569-87467588 Msa0481400:intron 40.0%
TGGTTTCAACACAGACGATA+TGG + chr3_3:87467598-87467617 Msa0481400:CDS 40.0%
TGGTGGTAAAGAAACTACTG+AGG + chr3_3:87467797-87467816 Msa0481400:CDS 40.0%
CTGCAGGACTATTAAAGGTA+AGG + chr3_3:87467877-87467896 Msa0481400:intron 40.0%
! GTGTTTTCACCTATGAAGCA+TGG + chr3_3:87468183-87468202 Msa0481400:intron 40.0%
TCACCTATGAAGCATGGATA+CGG + chr3_3:87468189-87468208 Msa0481400:intron 40.0%
GCACGAACACTTATCAGATT+AGG - chr3_3:87468324-87468343 None:intergenic 40.0%
! ACATATCAAACGAATGGGCT+AGG + chr3_3:87468687-87468706 Msa0481400:CDS 40.0%
! TACCTCGTCTATTAGCTCTT+CGG - chr3_3:87468741-87468760 None:intergenic 40.0%
GTTCACTTGAAAACAAGGGT+TGG + chr3_3:87468792-87468811 Msa0481400:three_prime_UTR 40.0%
! ATAAGCTAGAAGCCTTGTGT+AGG - chr3_3:87468864-87468883 None:intergenic 40.0%
GATAGGATATGTGTCTTGGA+TGG + chr3_3:87469099-87469118 Msa0481400:three_prime_UTR 40.0%
TGTCCGTATCCATGCTTCAT+AGG - chr3_3:87468195-87468214 None:intergenic 45.0%
ATGAAGCATGGATACGGACA+CGG + chr3_3:87468195-87468214 Msa0481400:intron 45.0%
TTATCAGATTAGGCGTGTCC+AGG - chr3_3:87468314-87468333 None:intergenic 45.0%
ATCCGAAGAGCTAATAGACG+AGG + chr3_3:87468736-87468755 Msa0481400:CDS 45.0%
!! GCTAATAGACGAGGTACTGA+AGG + chr3_3:87468745-87468764 Msa0481400:CDS 45.0%
CAGCTGTGAATTCCTACACA+AGG + chr3_3:87468849-87468868 Msa0481400:three_prime_UTR 45.0%
TCGAGTGATTCCACCACTTC+TGG + chr3_3:87469007-87469026 Msa0481400:three_prime_UTR 50.0%
!! GAAGAAGTGTCCAGAAGTGG+TGG - chr3_3:87469020-87469039 None:intergenic 50.0%
!! AGCGAAGAAGTGTCCAGAAG+TGG - chr3_3:87469023-87469042 None:intergenic 50.0%
TGTTGGCGTCTCAGTGTGTC+TGG - chr3_3:87468224-87468243 None:intergenic 55.0%
GATTAGGCGTGTCCAGGTGT+CGG - chr3_3:87468308-87468327 None:intergenic 55.0%
CACGACACGTGTCCGACACC+TGG + chr3_3:87468293-87468312 Msa0481400:intron 65.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 87466408 87469221 87466408 ID=Msa0481400;Name=Msa0481400
chr3_3 mRNA 87466408 87469221 87466408 ID=Msa0481400-mRNA-1;Parent=Msa0481400;Name=Msa0481400-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0|1|0.8|1|1|1|5|434|211
chr3_3 exon 87466408 87466475 87466408 ID=Msa0481400-mRNA-1:exon:25393;Parent=Msa0481400-mRNA-1
chr3_3 exon 87466568 87466817 87466568 ID=Msa0481400-mRNA-1:exon:25394;Parent=Msa0481400-mRNA-1
chr3_3 exon 87467578 87467619 87467578 ID=Msa0481400-mRNA-1:exon:25395;Parent=Msa0481400-mRNA-1
chr3_3 exon 87467720 87467893 87467720 ID=Msa0481400-mRNA-1:exon:25396;Parent=Msa0481400-mRNA-1
chr3_3 exon 87468686 87469221 87468686 ID=Msa0481400-mRNA-1:exon:25397;Parent=Msa0481400-mRNA-1
chr3_3 CDS 87466408 87466475 87466408 ID=Msa0481400-mRNA-1:cds;Parent=Msa0481400-mRNA-1
chr3_3 CDS 87466568 87466817 87466568 ID=Msa0481400-mRNA-1:cds;Parent=Msa0481400-mRNA-1
chr3_3 CDS 87467578 87467619 87467578 ID=Msa0481400-mRNA-1:cds;Parent=Msa0481400-mRNA-1
chr3_3 CDS 87467720 87467893 87467720 ID=Msa0481400-mRNA-1:cds;Parent=Msa0481400-mRNA-1
chr3_3 CDS 87468686 87468787 87468686 ID=Msa0481400-mRNA-1:cds;Parent=Msa0481400-mRNA-1
chr3_3 three_prime_UTR 87468788 87469221 87468788 ID=Msa0481400-mRNA-1:three_prime_utr;Parent=Msa0481400-mRNA-1
Gene Sequence

>Msa0481400

TTGTGCACATCCATTCATTTAGATTCAATTAAAATTAATCATCTATCTATGGCAATTTCAAAAGAAAGATATGCTGTTGTTACAGGATCAAATAAAGGTATAGGATTGGAAACTGTTAAAAGATTGGCTTCAAATGGAATTAAAGTTGTTCTTACAGCTAGAAATCAGAAAAGAGGGATTCAAGCTTTTGAGAAATTGAAGAAAGAATTTGAATTTTGTAATCTTGTTGTTTTTCATCAACTTGATGTTACTGATCCTTTTAGTATTGCTTCATTGGTTGAGTTTGTTAAGACTCAGTTTGGAAGACTTGATATCTTGGTAAATAATGCAGGAATCAATGGTTTCAACACAGACGATATGGTTGAACCAATTATTAATTGGAGAGAACTGAGTCAAACTTATGAGATGGCAGAAAATTGCATAATAACAAATTACTATGGTGGTAAAGAAACTACTGAGGCTTTTCTTCCTCTTCTTCAGTTATCTGATTCACCTGTGATTGTTAATGTTTCTTCTACTGCAGGACTATTAAAGTACATATCAAACGAATGGGCTAGGAGTGTGTTAGACGATACTGAAAATCTATCCGAAGAGCTAATAGACGAGGTACTGAAGGAGTTTCTGAAAGACTTCTAA
Protein sequence

>Msa0481400

LCTSIHLDSIKINHLSMAISKERYAVVTGSNKGIGLETVKRLASNGIKVVLTARNQKRGIQAFEKLKKEFEFCNLVVFHQLDVTDPFSIASLVEFVKTQFGRLDILVNNAGINGFNTDDMVEPIINWRELSQTYEMAENCIITNYYGGKETTEAFLPLLQLSDSPVIVNVSSTAGLLKYISNEWARSVLDDTENLSEELIDEVLKEFLKDF*