Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1270050 | XP_003627594.1 | 97.222 | 288 | 7 | 1 | 1 | 288 | 1 | 287 | 0.0 | 575 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1270050 | sp|Q9M2E2|SDR1_ARATH | 57.095 | 296 | 119 | 3 | 1 | 288 | 1 | 296 | 2.83e-116 | 339 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1270050 | G7LBT2 | 97.222 | 288 | 7 | 1 | 1 | 288 | 1 | 287 | 0.0 | 575 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0001830 | Msa1270050 | 0.883230 | 5.348766e-71 | -8.615850e-47 |
| Msa0090380 | Msa1270050 | 0.806490 | 8.021312e-50 | -8.615850e-47 |
| Msa0105410 | Msa1270050 | 0.807945 | 3.944613e-50 | -8.615850e-47 |
| Msa0613290 | Msa1270050 | 0.801760 | 7.732346e-49 | -8.615850e-47 |
| Msa0613340 | Msa1270050 | 0.813523 | 2.451956e-51 | -8.615850e-47 |
| Msa0631320 | Msa1270050 | 0.846682 | 1.862845e-59 | -8.615850e-47 |
| Msa0641130 | Msa1270050 | 0.811525 | 6.702770e-51 | -8.615850e-47 |
| Msa0646240 | Msa1270050 | 0.803039 | 4.215946e-49 | -8.615850e-47 |
| Msa0653830 | Msa1270050 | 0.822240 | 2.632899e-53 | -8.615850e-47 |
| Msa0679670 | Msa1270050 | 0.816218 | 6.194859e-52 | -8.615850e-47 |
| Msa0711150 | Msa1270050 | 0.809843 | 1.548933e-50 | -8.615850e-47 |
| Msa0744640 | Msa1270050 | 0.818496 | 1.901402e-52 | -8.615850e-47 |
| Msa0795730 | Msa1270050 | 0.801179 | 1.017233e-48 | -8.615850e-47 |
| Msa0802240 | Msa1270050 | 0.810343 | 1.208164e-50 | -8.615850e-47 |
| Msa0812530 | Msa1270050 | 0.802875 | 4.557454e-49 | -8.615850e-47 |
| Msa0822500 | Msa1270050 | 0.807312 | 5.375982e-50 | -8.615850e-47 |
| Msa0841730 | Msa1270050 | 0.818342 | 2.061000e-52 | -8.615850e-47 |
| Msa0190930 | Msa1270050 | 0.809130 | 2.203482e-50 | -8.615850e-47 |
| Msa0233250 | Msa1270050 | 0.820407 | 6.971946e-53 | -8.615850e-47 |
| Msa0233970 | Msa1270050 | 0.817034 | 4.064863e-52 | -8.615850e-47 |
| Msa1231710 | Msa1270050 | 0.803803 | 2.928393e-49 | -8.615850e-47 |
| Msa1231840 | Msa1270050 | 0.815497 | 8.971169e-52 | -8.615850e-47 |
| Msa1236470 | Msa1270050 | 0.808578 | 2.891447e-50 | -8.615850e-47 |
| Msa1238600 | Msa1270050 | 0.812605 | 3.897127e-51 | -8.615850e-47 |
| Msa1256810 | Msa1270050 | 0.810013 | 1.423644e-50 | -8.615850e-47 |
| Msa1270050 | Msa1271130 | 0.807133 | 5.866406e-50 | -8.615850e-47 |
| Msa1270050 | Msa1277780 | 0.812300 | 4.543668e-51 | -8.615850e-47 |
| Msa1270050 | Msa1307160 | 0.806656 | 7.397853e-50 | -8.615850e-47 |
| Msa1270050 | Msa1312440 | 0.811593 | 6.477797e-51 | -8.615850e-47 |
| Msa1270050 | Msa1316360 | 0.804952 | 1.687019e-49 | -8.615850e-47 |
| Msa1270050 | Msa1355890 | 0.825080 | 5.695800e-54 | -8.615850e-47 |
| Msa1270050 | Msa1380220 | 0.817491 | 3.208237e-52 | -8.615850e-47 |
| Msa1270050 | Msa1386000 | 0.825718 | 4.024317e-54 | -8.615850e-47 |
| Msa1270050 | Msa1407760 | 0.811899 | 5.558697e-51 | -8.615850e-47 |
| Msa1270050 | Msa1433410 | 0.805437 | 1.335401e-49 | -8.615850e-47 |
| Msa1270050 | Msa1437730 | 0.807722 | 4.398413e-50 | -8.615850e-47 |
| Msa1270050 | Msa1443620 | 0.804499 | 2.097761e-49 | -8.615850e-47 |
| Msa1270050 | Msa1443640 | 0.802598 | 5.201017e-49 | -8.615850e-47 |
| Msa1270050 | Msa1454450 | 0.810181 | 1.309830e-50 | -8.615850e-47 |
| Msa0389210 | Msa1270050 | 0.855924 | 4.550018e-62 | -8.615850e-47 |
| Msa0469620 | Msa1270050 | -0.807229 | 5.598670e-50 | -8.615850e-47 |
| Msa0481400 | Msa1270050 | 0.893157 | 8.164985e-75 | -8.615850e-47 |
| Msa1023310 | Msa1270050 | 0.802634 | 5.111706e-49 | -8.615850e-47 |
| Msa1029050 | Msa1270050 | 0.815693 | 8.113413e-52 | -8.615850e-47 |
| Msa1059470 | Msa1270050 | 0.828748 | 7.565417e-55 | -8.615850e-47 |
| Msa1062040 | Msa1270050 | 0.820553 | 6.454406e-53 | -8.615850e-47 |
| Msa0272400 | Msa1270050 | 0.828059 | 1.109611e-54 | -8.615850e-47 |
| Msa0311500 | Msa1270050 | 0.823015 | 1.738748e-53 | -8.615850e-47 |
| Msa0311510 | Msa1270050 | 0.812818 | 3.501711e-51 | -8.615850e-47 |
| Msa0312590 | Msa1270050 | 0.823104 | 1.657908e-53 | -8.615850e-47 |
| Msa0332190 | Msa1270050 | 0.814215 | 1.725325e-51 | -8.615850e-47 |
| Msa0340380 | Msa1270050 | 0.807766 | 4.305639e-50 | -8.615850e-47 |
| Msa1107200 | Msa1270050 | 0.829713 | 4.412887e-55 | -8.615850e-47 |
| Msa1109090 | Msa1270050 | 0.816946 | 4.254349e-52 | -8.615850e-47 |
| Msa1122310 | Msa1270050 | -0.800019 | 1.753602e-48 | -8.615850e-47 |
| Msa1150780 | Msa1270050 | 0.802482 | 5.493635e-49 | -8.615850e-47 |
| Msa1176170 | Msa1270050 | 0.803163 | 3.973948e-49 | -8.615850e-47 |
| Msa1176380 | Msa1270050 | 0.800677 | 1.288057e-48 | -8.615850e-47 |
| Msa1180720 | Msa1270050 | 0.807854 | 4.123538e-50 | -8.615850e-47 |
| Msa1184690 | Msa1270050 | 0.821947 | 3.079057e-53 | -8.615850e-47 |
| Msa1191440 | Msa1270050 | 0.977403 | 1.035174e-143 | -8.615850e-47 |
| Msa1206270 | Msa1270050 | 0.816320 | 5.875907e-52 | -8.615850e-47 |
| Msa1206290 | Msa1270050 | 0.803442 | 3.479674e-49 | -8.615850e-47 |
| Msa0534870 | Msa1270050 | 0.840918 | 6.511101e-58 | -8.615850e-47 |
| Msa0545050 | Msa1270050 | 0.828376 | 9.304696e-55 | -8.615850e-47 |
| Msa0574930 | Msa1270050 | 0.829018 | 6.507116e-55 | -8.615850e-47 |
| Msa0600590 | Msa1270050 | 0.835929 | 1.260037e-56 | -8.615850e-47 |
| Msa0870510 | Msa1270050 | 0.828347 | 9.455785e-55 | -8.615850e-47 |
| Msa0894710 | Msa1270050 | 0.806119 | 9.604662e-50 | -8.615850e-47 |
| Msa0930740 | Msa1270050 | 0.865318 | 6.440042e-65 | -8.615850e-47 |
| Msa0944610 | Msa1270050 | 0.811183 | 7.953841e-51 | -8.615850e-47 |
| Msa0945130 | Msa1270050 | 0.821739 | 3.439453e-53 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1270050 | MtrunA17_Chr8g0349741 | 97.222 | 288 | 7 | 1 | 1 | 288 | 1 | 287 | 0.0 | 575 |
| Msa1270050 | MtrunA17_Chr7g0247011 | 66.319 | 288 | 95 | 2 | 1 | 288 | 1 | 286 | 1.05e-138 | 392 |
| Msa1270050 | MtrunA17_Chr7g0247031 | 64.211 | 285 | 97 | 4 | 6 | 288 | 7 | 288 | 3.68e-127 | 363 |
| Msa1270050 | MtrunA17_Chr8g0349781 | 58.667 | 300 | 110 | 4 | 1 | 288 | 1 | 298 | 4.66e-122 | 351 |
| Msa1270050 | MtrunA17_Chr8g0349811 | 57.000 | 300 | 115 | 4 | 1 | 288 | 1 | 298 | 9.58e-119 | 342 |
| Msa1270050 | MtrunA17_Chr8g0349851 | 57.432 | 296 | 111 | 4 | 6 | 288 | 6 | 299 | 1.49e-118 | 342 |
| Msa1270050 | MtrunA17_Chr8g0349801 | 56.000 | 300 | 118 | 4 | 1 | 288 | 1 | 298 | 7.77e-116 | 335 |
| Msa1270050 | MtrunA17_Chr8g0349761 | 59.794 | 291 | 103 | 5 | 7 | 285 | 8 | 296 | 2.15e-115 | 334 |
| Msa1270050 | MtrunA17_Chr8g0349771 | 56.667 | 300 | 116 | 4 | 1 | 288 | 1 | 298 | 1.34e-114 | 332 |
| Msa1270050 | MtrunA17_Chr7g0247041 | 57.586 | 290 | 114 | 4 | 7 | 288 | 6 | 294 | 1.94e-113 | 328 |
| Msa1270050 | MtrunA17_Chr8g0349831 | 55.743 | 296 | 116 | 4 | 6 | 288 | 7 | 300 | 3.46e-111 | 323 |
| Msa1270050 | MtrunA17_Chr8g0349671 | 56.000 | 300 | 117 | 6 | 1 | 288 | 1 | 297 | 3.39e-109 | 318 |
| Msa1270050 | MtrunA17_Chr8g0349701 | 54.576 | 295 | 125 | 5 | 1 | 288 | 1 | 293 | 5.96e-109 | 317 |
| Msa1270050 | MtrunA17_Chr8g0349751 | 54.455 | 303 | 119 | 5 | 1 | 288 | 51 | 349 | 3.62e-108 | 317 |
| Msa1270050 | MtrunA17_Chr8g0349721 | 54.237 | 295 | 126 | 5 | 1 | 288 | 1 | 293 | 3.98e-108 | 315 |
| Msa1270050 | MtrunA17_Chr8g0349641 | 54.333 | 300 | 123 | 4 | 1 | 288 | 1 | 298 | 6.06e-106 | 310 |
| Msa1270050 | MtrunA17_Chr5g0425391 | 50.000 | 298 | 136 | 5 | 1 | 288 | 1 | 295 | 5.38e-98 | 290 |
| Msa1270050 | MtrunA17_Chr8g0349651 | 54.340 | 265 | 107 | 6 | 35 | 288 | 14 | 275 | 3.97e-91 | 271 |
| Msa1270050 | MtrunA17_Chr3g0118511 | 49.485 | 291 | 126 | 6 | 7 | 279 | 3 | 290 | 5.93e-82 | 249 |
| Msa1270050 | MtrunA17_Chr7g0267881 | 51.515 | 231 | 91 | 2 | 7 | 233 | 9 | 222 | 5.01e-76 | 231 |
| Msa1270050 | MtrunA17_Chr3g0131521 | 42.808 | 292 | 138 | 8 | 4 | 283 | 32 | 306 | 2.47e-62 | 199 |
| Msa1270050 | MtrunA17_Chr1g0160511 | 43.345 | 293 | 135 | 9 | 4 | 283 | 37 | 311 | 2.47e-58 | 189 |
| Msa1270050 | MtrunA17_Chr8g0385581 | 38.983 | 295 | 148 | 9 | 4 | 283 | 34 | 311 | 1.38e-51 | 171 |
| Msa1270050 | MtrunA17_Chr5g0437701 | 53.488 | 129 | 59 | 1 | 160 | 288 | 1 | 128 | 3.66e-44 | 146 |
| Msa1270050 | MtrunA17_Chr4g0045691 | 35.465 | 172 | 100 | 3 | 127 | 288 | 3 | 173 | 7.40e-29 | 108 |
| Msa1270050 | MtrunA17_Chr4g0045711 | 46.739 | 92 | 45 | 2 | 4 | 91 | 19 | 110 | 8.08e-15 | 69.7 |
| Msa1270050 | MtrunA17_Chr7g0270461 | 36.957 | 92 | 53 | 1 | 7 | 98 | 49 | 135 | 4.71e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1270050 | AT3G61220.1 | 57.095 | 296 | 119 | 3 | 1 | 288 | 1 | 296 | 2.87e-117 | 339 |
| Msa1270050 | AT3G61220.2 | 57.586 | 290 | 115 | 3 | 7 | 288 | 14 | 303 | 4.70e-116 | 336 |
| Msa1270050 | AT3G61220.3 | 57.586 | 290 | 115 | 3 | 7 | 288 | 38 | 327 | 1.34e-115 | 336 |
| Msa1270050 | AT2G24190.1 | 54.027 | 298 | 125 | 4 | 1 | 288 | 1 | 296 | 5.61e-111 | 323 |
| Msa1270050 | AT2G24190.2 | 54.452 | 292 | 121 | 4 | 7 | 288 | 12 | 301 | 3.94e-110 | 321 |
| Msa1270050 | AT1G01800.1 | 54.730 | 296 | 123 | 4 | 3 | 288 | 1 | 295 | 4.24e-107 | 313 |
| Msa1270050 | AT1G01800.2 | 54.106 | 207 | 85 | 3 | 91 | 288 | 55 | 260 | 3.37e-69 | 215 |
| Msa1270050 | AT5G51030.1 | 40.541 | 296 | 146 | 10 | 4 | 286 | 35 | 313 | 5.39e-60 | 193 |
| Msa1270050 | AT3G59710.3 | 41.489 | 282 | 143 | 7 | 4 | 280 | 16 | 280 | 1.06e-58 | 189 |
| Msa1270050 | AT3G59710.1 | 41.489 | 282 | 143 | 7 | 4 | 280 | 26 | 290 | 1.45e-58 | 189 |
| Msa1270050 | AT5G61830.1 | 40.690 | 290 | 146 | 6 | 6 | 283 | 36 | 311 | 7.40e-58 | 188 |
| Msa1270050 | AT5G61830.2 | 40.690 | 290 | 146 | 6 | 6 | 283 | 36 | 311 | 7.40e-58 | 188 |
| Msa1270050 | AT3G59710.2 | 41.489 | 282 | 143 | 7 | 4 | 280 | 45 | 309 | 7.79e-58 | 188 |
| Msa1270050 | AT5G50700.1 | 30.519 | 154 | 95 | 3 | 7 | 160 | 48 | 189 | 4.10e-13 | 69.3 |
| Msa1270050 | AT5G50600.1 | 30.519 | 154 | 95 | 3 | 7 | 160 | 48 | 189 | 4.10e-13 | 69.3 |
| Msa1270050 | AT4G24050.2 | 29.344 | 259 | 118 | 12 | 9 | 250 | 37 | 247 | 1.77e-12 | 66.6 |
| Msa1270050 | AT4G24050.1 | 29.344 | 259 | 118 | 12 | 9 | 250 | 37 | 247 | 1.91e-12 | 67.0 |
| Msa1270050 | AT5G50130.8 | 25.517 | 290 | 169 | 10 | 6 | 281 | 3 | 259 | 4.75e-12 | 65.9 |
| Msa1270050 | AT5G50130.9 | 26.141 | 241 | 145 | 8 | 9 | 249 | 39 | 246 | 9.02e-11 | 61.6 |
Find 58 sgRNAs with CRISPR-Local
Find 143 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTACTGCAGGACTATTAA+AGG | 0.190820 | 8_3:-3419960 | Msa1270050:intron |
| TTCAGATATGCTGTTGTTAC+AGG | 0.201198 | 8_3:-3421273 | Msa1270050:intron |
| AGTTCCCTCCAATTAATAAT+TGG | 0.237001 | 8_3:+3420106 | None:intergenic |
| TCGAGTGATTCCACCACTTC+TGG | 0.273816 | 8_3:-3418825 | Msa1270050:CDS |
| ACCAATGAAGCAATACTAAA+AGG | 0.304929 | 8_3:+3421081 | None:intergenic |
| AGGTTGAACCAATTATTAAT+TGG | 0.308846 | 8_3:-3420114 | Msa1270050:CDS |
| GGTATATTATCTGTTGAAAA+TGG | 0.316053 | 8_3:-3418879 | Msa1270050:CDS |
| TAAAATTAATCATCTATCTA+TGG | 0.342933 | 8_3:-3421399 | Msa1270050:exon |
| ACGCAATTAATACATAAGTT+TGG | 0.345471 | 8_3:+3418942 | None:intergenic |
| ATGTATTAATTGCGTTTGTC+CGG | 0.349424 | 8_3:-3418934 | Msa1270050:CDS |
| GTTTGGAAGACTTGATATCT+TGG | 0.353617 | 8_3:-3421040 | Msa1270050:intron |
| ATTGGAAACTGTTAAAAGAT+TGG | 0.358632 | 8_3:-3421232 | Msa1270050:CDS |
| TTTAATTGAATCTAAATGAA+TGG | 0.373999 | 8_3:+3421418 | None:intergenic |
| ACAGGATCAAATAAAGGTAT+AGG | 0.380633 | 8_3:-3421255 | Msa1270050:CDS |
| TGGTTTCAACACAGACGATA+TGG | 0.383538 | 8_3:-3420237 | Msa1270050:intron |
| AATAAACAGCAATGAACAAA+TGG | 0.385084 | 8_3:+3418690 | None:intergenic |
| GTTAAAAGATTGGCTTCAAA+TGG | 0.389901 | 8_3:-3421222 | Msa1270050:CDS |
| GAATTTGTTAAGACTCAGTT+TGG | 0.409572 | 8_3:-3421057 | Msa1270050:CDS |
| TGCATAATAACAAATTACTA+TGG | 0.411597 | 8_3:-3420055 | Msa1270050:CDS |
| GTAAATAATGCAGGAATCAA+TGG | 0.443701 | 8_3:-3420257 | Msa1270050:CDS |
| GTTAATGTTTCTTCTACTGC+AGG | 0.448645 | 8_3:-3419971 | Msa1270050:CDS |
| ATCAAATAAAGGTATAGGAT+TGG | 0.463443 | 8_3:-3421250 | Msa1270050:CDS |
| ATATGATAGGATATGTGTCT+TGG | 0.480820 | 8_3:-3418737 | Msa1270050:three_prime_UTR |
| GTTGTTACAGGATCAAATAA+AGG | 0.492642 | 8_3:-3421261 | Msa1270050:CDS |
| TGTATTAATTGCGTTTGTCC+GGG | 0.494685 | 8_3:-3418933 | Msa1270050:CDS |
| CTATGGCAATTTCAAAAGAA+AGG | 0.498850 | 8_3:-3421382 | Msa1270050:intron |
| TTGAACCAATTATTAATTGG+AGG | 0.500877 | 8_3:-3420111 | Msa1270050:CDS |
| TACCTCGTCTATTAGCTCTT+CGG | 0.500938 | 8_3:+3419094 | None:intergenic |
| TTATTAATCAAATGAAATGT+GGG | 0.504860 | 8_3:-3418672 | Msa1270050:three_prime_UTR |
| TTCAGTACATATCAAACGAA+TGG | 0.506130 | 8_3:-3419151 | Msa1270050:intron |
| ACTTCTAGAAATCAGAAAAG+AGG | 0.511868 | 8_3:-3421183 | Msa1270050:CDS |
| TTTATTAATCAAATGAAATG+TGG | 0.512170 | 8_3:-3418673 | Msa1270050:three_prime_UTR |
| CATGTGCAGGTAAATAATGC+AGG | 0.516920 | 8_3:-3420266 | Msa1270050:intron |
| CTTCTAGAAATCAGAAAAGA+GGG | 0.518381 | 8_3:-3421182 | Msa1270050:CDS |
| ACATATCAAACGAATGGGCT+AGG | 0.522049 | 8_3:-3419145 | Msa1270050:CDS |
| TGAACCAATTATTAATTGGA+GGG | 0.526022 | 8_3:-3420110 | Msa1270050:CDS |
| GAATCAGATAACTGAAGAAG+AGG | 0.526731 | 8_3:+3420004 | None:intergenic |
| TCAGTACATATCAAACGAAT+GGG | 0.528974 | 8_3:-3419150 | Msa1270050:intron |
| GAAGAAACATTAACAATCAC+AGG | 0.532332 | 8_3:+3419980 | None:intergenic |
| TTATAGGCACAAAGATATGT+TGG | 0.548863 | 8_3:+3419017 | None:intergenic |
| ACAAGGTTCACTTGAAAACA+AGG | 0.549206 | 8_3:-3419045 | Msa1270050:CDS |
| ATAAACAGCAATGAACAAAT+GGG | 0.555758 | 8_3:+3418691 | None:intergenic |
| GCTAATAGACGAGGTACTGA+AGG | 0.563423 | 8_3:-3419087 | Msa1270050:CDS |
| TTTCAATGTATTAAAATGGG+GGG | 0.578258 | 8_3:-3418760 | Msa1270050:three_prime_UTR |
| GATAGGATATGTGTCTTGGA+TGG | 0.587395 | 8_3:-3418733 | Msa1270050:three_prime_UTR |
| AGCGAAGAAGTGTCCAGAAG+TGG | 0.605903 | 8_3:+3418812 | None:intergenic |
| GTTCACTTGAAAACAAGGGT+TGG | 0.606906 | 8_3:-3419040 | Msa1270050:CDS |
| ACTGAGTCAAACTTATGAGA+TGG | 0.614913 | 8_3:-3420086 | Msa1270050:CDS |
| TATTAATCAAATGAAATGTG+GGG | 0.615096 | 8_3:-3418671 | Msa1270050:three_prime_UTR |
| ATAAGCTAGAAGCCTTGTGT+AGG | 0.622776 | 8_3:+3418971 | None:intergenic |
| ATAATAACAAATTACTATGG+TGG | 0.628363 | 8_3:-3420052 | Msa1270050:CDS |
| CAGCTGTGAATTCCTACACA+AGG | 0.629636 | 8_3:-3418983 | Msa1270050:CDS |
| TTTCTGAAAGACTTCAAACA+AGG | 0.631673 | 8_3:-3419062 | Msa1270050:CDS |
| ACAGATATGAATAGAAACAC+AGG | 0.645826 | 8_3:-3418900 | Msa1270050:CDS |
| GAAGAAGTGTCCAGAAGTGG+TGG | 0.653264 | 8_3:+3418815 | None:intergenic |
| CAAGGTTCACTTGAAAACAA+GGG | 0.660578 | 8_3:-3419044 | Msa1270050:CDS |
| ATCCGAAGAGCTAATAGACG+AGG | 0.706099 | 8_3:-3419096 | Msa1270050:CDS |
| TGGTGGTAAAGAAACTACTG+AGG | 0.726056 | 8_3:-3420035 | Msa1270050:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATATATTTTTTTAGTTATCT+TGG | - | chr8_3:3419240-3419259 | Msa1270050:intron | 10.0% |
| !! | TTTAATTGAATCTAAATGAA+TGG | + | chr8_3:3418638-3418657 | None:intergenic | 15.0% |
| !! | TAAAATTAATCATCTATCTA+TGG | - | chr8_3:3418654-3418673 | Msa1270050:three_prime_UTR | 15.0% |
| !! | CTTTGATAAAAGAAATTAAA+GGG | + | chr8_3:3418737-3418756 | None:intergenic | 15.0% |
| !! | ATTATGAATTACCATTTATT+TGG | - | chr8_3:3419152-3419171 | Msa1270050:intron | 15.0% |
| !! | CTCAAAAAATTATTGTATAA+AGG | - | chr8_3:3420283-3420302 | Msa1270050:intron | 15.0% |
| !! | TTAATTTCTCAAATTATTAG+TGG | + | chr8_3:3420468-3420487 | None:intergenic | 15.0% |
| !!! | ATGTTTTTCATTAGTTAATT+TGG | + | chr8_3:3420704-3420723 | None:intergenic | 15.0% |
| !! | TTTATTAATCAAATGAAATG+TGG | - | chr8_3:3421380-3421399 | Msa1270050:intron | 15.0% |
| !! | TTATTAATCAAATGAAATGT+GGG | - | chr8_3:3421381-3421400 | Msa1270050:intron | 15.0% |
| !!! | AATTTCTACTAAATTTTAGT+TGG | - | chr8_3:3421424-3421443 | Msa1270050:five_prime_UTR | 15.0% |
| !! | GCTTTGATAAAAGAAATTAA+AGG | + | chr8_3:3418738-3418757 | None:intergenic | 20.0% |
| !! | TTCTTGATTCTTAATTATCT+AGG | - | chr8_3:3419213-3419232 | Msa1270050:intron | 20.0% |
| !! | CTAAGTTATTTATCAAAACA+AGG | + | chr8_3:3419303-3419322 | None:intergenic | 20.0% |
| !!! | ATTATCACAAATTTTAGTCA+TGG | + | chr8_3:3419730-3419749 | None:intergenic | 20.0% |
| !! | AAAATTCAAGGCTATTATTA+TGG | + | chr8_3:3419767-3419786 | None:intergenic | 20.0% |
| !!! | TTTTTTGTATATGACTATTC+AGG | - | chr8_3:3419919-3419938 | Msa1270050:intron | 20.0% |
| !! | TGCATAATAACAAATTACTA+TGG | - | chr8_3:3419998-3420017 | Msa1270050:CDS | 20.0% |
| !! | ATAATAACAAATTACTATGG+TGG | - | chr8_3:3420001-3420020 | Msa1270050:CDS | 20.0% |
| !!! | CAATATTTTTCGTTTTTTAG+AGG | + | chr8_3:3420204-3420223 | None:intergenic | 20.0% |
| !!! | AATTGGTACTTTTCATTTTT+CGG | - | chr8_3:3420342-3420361 | Msa1270050:intron | 20.0% |
| !! | AAAAAATGATGAACTCTATA+TGG | + | chr8_3:3420371-3420390 | None:intergenic | 20.0% |
| !! | TTAAGTGTAATTATATGTGT+TGG | - | chr8_3:3420484-3420503 | Msa1270050:intron | 20.0% |
| !!! | AGGTTTTCAATGTATTAAAA+TGG | - | chr8_3:3421289-3421308 | Msa1270050:intron | 20.0% |
| !!! | GGTTTTCAATGTATTAAAAT+GGG | - | chr8_3:3421290-3421309 | Msa1270050:intron | 20.0% |
| !!! | GTTTTCAATGTATTAAAATG+GGG | - | chr8_3:3421291-3421310 | Msa1270050:intron | 20.0% |
| !!! | TTTTCAATGTATTAAAATGG+GGG | - | chr8_3:3421292-3421311 | Msa1270050:intron | 20.0% |
| !! | TATTAATCAAATGAAATGTG+GGG | - | chr8_3:3421382-3421401 | Msa1270050:intron | 20.0% |
| !! | AGGTACCTTTTATGTTTATT+TGG | - | chr8_3:3418691-3418710 | Msa1270050:three_prime_UTR | 25.0% |
| ! | TTGGTCCAAATAAACATAAA+AGG | + | chr8_3:3418699-3418718 | None:intergenic | 25.0% |
| !! | AGGGATCTTTTAACATTTAT+TGG | + | chr8_3:3418718-3418737 | None:intergenic | 25.0% |
| !! | ATCAAATAAAGGTATAGGAT+TGG | - | chr8_3:3418803-3418822 | Msa1270050:CDS | 25.0% |
| ! | ATTGGAAACTGTTAAAAGAT+TGG | - | chr8_3:3418821-3418840 | Msa1270050:CDS | 25.0% |
| !!! | AGAAAATTGGCTTTACATTA+AGG | + | chr8_3:3419052-3419071 | None:intergenic | 25.0% |
| !! | TTAATGTAAAGCCAATTTTC+TGG | - | chr8_3:3419051-3419070 | Msa1270050:CDS | 25.0% |
| ! | ATCATAATGATCCAGAAAAT+TGG | + | chr8_3:3419065-3419084 | None:intergenic | 25.0% |
| ! | TTTATCCAAGTGTTCAAAAA+TGG | + | chr8_3:3419379-3419398 | None:intergenic | 25.0% |
| ! | CTGTTTGAAAGAACTTATAA+GGG | + | chr8_3:3419496-3419515 | None:intergenic | 25.0% |
| ! | ACTGTTTGAAAGAACTTATA+AGG | + | chr8_3:3419497-3419516 | None:intergenic | 25.0% |
| ! | ACAAACTTTGGATAAACTAA+TGG | - | chr8_3:3419682-3419701 | Msa1270050:intron | 25.0% |
| !!! | CTTGTTTTCTTTAACTGTTT+GGG | - | chr8_3:3419847-3419866 | Msa1270050:intron | 25.0% |
| ! | AAAAAACAAAGCTTAATCAG+AGG | + | chr8_3:3419903-3419922 | None:intergenic | 25.0% |
| ! | AGGTTGAACCAATTATTAAT+TGG | - | chr8_3:3419939-3419958 | Msa1270050:intron | 25.0% |
| ! | TTGAACCAATTATTAATTGG+AGG | - | chr8_3:3419942-3419961 | Msa1270050:intron | 25.0% |
| ! | TGAACCAATTATTAATTGGA+GGG | - | chr8_3:3419943-3419962 | Msa1270050:intron | 25.0% |
| !! | AAGTCTTTGAATTTTTCTGA+AGG | + | chr8_3:3420174-3420193 | None:intergenic | 25.0% |
| !!! | ATTGGTACTTTTCATTTTTC+GGG | - | chr8_3:3420343-3420362 | Msa1270050:intron | 25.0% |
| ! | ACTAACATAAGACAGATATA+AGG | + | chr8_3:3420638-3420657 | None:intergenic | 25.0% |
| ! | TTGATGTGTATTTCATTAGT+AGG | + | chr8_3:3420677-3420696 | None:intergenic | 25.0% |
| ! | ACTAATGAAATACACATCAA+AGG | - | chr8_3:3420677-3420696 | Msa1270050:intron | 25.0% |
| ! | ACAGTTTATTTATGTTGTTC+AGG | + | chr8_3:3420865-3420884 | None:intergenic | 25.0% |
| ! | ACGCAATTAATACATAAGTT+TGG | + | chr8_3:3421114-3421133 | None:intergenic | 25.0% |
| !!! | ATATCTGTTTTAACAAAACC+CGG | + | chr8_3:3421141-3421160 | None:intergenic | 25.0% |
| ! | GGTATATTATCTGTTGAAAA+TGG | - | chr8_3:3421174-3421193 | Msa1270050:CDS | 25.0% |
| ! | TTTCAATGTATTAAAATGGG+GGG | - | chr8_3:3421293-3421312 | Msa1270050:intron | 25.0% |
| ! | ATAAACAGCAATGAACAAAT+GGG | + | chr8_3:3421365-3421384 | None:intergenic | 25.0% |
| ! | AATAAACAGCAATGAACAAA+TGG | + | chr8_3:3421366-3421385 | None:intergenic | 25.0% |
| CTATGGCAATTTCAAAAGAA+AGG | - | chr8_3:3418671-3418690 | Msa1270050:three_prime_UTR | 30.0% | |
| GTTGTTACAGGATCAAATAA+AGG | - | chr8_3:3418792-3418811 | Msa1270050:CDS | 30.0% | |
| ! | ACAGGATCAAATAAAGGTAT+AGG | - | chr8_3:3418798-3418817 | Msa1270050:CDS | 30.0% |
| !! | GTTAAAAGATTGGCTTCAAA+TGG | - | chr8_3:3418831-3418850 | Msa1270050:CDS | 30.0% |
| ! | ACTTCTAGAAATCAGAAAAG+AGG | - | chr8_3:3418870-3418889 | Msa1270050:CDS | 30.0% |
| ! | CTTCTAGAAATCAGAAAAGA+GGG | - | chr8_3:3418871-3418890 | Msa1270050:CDS | 30.0% |
| ! | TCCTTTTAGTATTGCTTCAT+TGG | - | chr8_3:3418971-3418990 | Msa1270050:CDS | 30.0% |
| ACCAATGAAGCAATACTAAA+AGG | + | chr8_3:3418975-3418994 | None:intergenic | 30.0% | |
| !! | GAATTTGTTAAGACTCAGTT+TGG | - | chr8_3:3418996-3419015 | Msa1270050:CDS | 30.0% |
| ! | TACCATTTATTTGGTGAATC+TGG | - | chr8_3:3419161-3419180 | Msa1270050:intron | 30.0% |
| TTCTTGATTCTCATTCTCAT+AGG | - | chr8_3:3419277-3419296 | Msa1270050:intron | 30.0% | |
| TGATAAAAGCTCTTAGCAAA+AGG | + | chr8_3:3419332-3419351 | None:intergenic | 30.0% | |
| !!! | ATAAGCCATTTTTGAACACT+TGG | - | chr8_3:3419371-3419390 | Msa1270050:intron | 30.0% |
| !!! | ACTTATTTTAGCTGATCTAC+TGG | + | chr8_3:3419536-3419555 | None:intergenic | 30.0% |
| GCACAAACACTTCAAATTAA+AGG | - | chr8_3:3419629-3419648 | Msa1270050:intron | 30.0% | |
| GTTTATCCAAAGTTTGTAAC+AGG | + | chr8_3:3419679-3419698 | None:intergenic | 30.0% | |
| GTAAATAATGCAGGAATCAA+TGG | - | chr8_3:3419796-3419815 | Msa1270050:intron | 30.0% | |
| !! | GCTTGTTTTCTTTAACTGTT+TGG | - | chr8_3:3419846-3419865 | Msa1270050:intron | 30.0% |
| ! | ACTGTTTGGGATGATAATAT+TGG | - | chr8_3:3419860-3419879 | Msa1270050:intron | 30.0% |
| AGTTCCCTCCAATTAATAAT+TGG | + | chr8_3:3419950-3419969 | None:intergenic | 30.0% | |
| GAAGAAACATTAACAATCAC+AGG | + | chr8_3:3420076-3420095 | None:intergenic | 30.0% | |
| CTATCAGAGATTATTTAGAC+TGG | + | chr8_3:3420251-3420270 | None:intergenic | 30.0% | |
| !! | TTGGTACTTTTCATTTTTCG+GGG | - | chr8_3:3420344-3420363 | Msa1270050:intron | 30.0% |
| ! | TCATAGGTGAAAACACATTT+TGG | + | chr8_3:3420400-3420419 | None:intergenic | 30.0% |
| TCTCAAATTATTAGTGGTGT+TGG | + | chr8_3:3420462-3420481 | None:intergenic | 30.0% | |
| ! | GTAATTATATGTGTTGGTGT+CGG | - | chr8_3:3420490-3420509 | Msa1270050:intron | 30.0% |
| ACATACAAGAAAAACATTCG+AGG | - | chr8_3:3420741-3420760 | Msa1270050:intron | 30.0% | |
| !! | CGAGGATGTTTTTATAAGTT+TGG | - | chr8_3:3420759-3420778 | Msa1270050:intron | 30.0% |
| GGTTAATTCAGAGTCTATAA+CGG | - | chr8_3:3420780-3420799 | Msa1270050:intron | 30.0% | |
| CTGATGTAAAGTGGTAATTT+CGG | + | chr8_3:3420832-3420851 | None:intergenic | 30.0% | |
| ACATTGTAACTGATGTAAAG+TGG | + | chr8_3:3420841-3420860 | None:intergenic | 30.0% | |
| TTCAGTACATATCAAACGAA+TGG | - | chr8_3:3420902-3420921 | Msa1270050:intron | 30.0% | |
| TCAGTACATATCAAACGAAT+GGG | - | chr8_3:3420903-3420922 | Msa1270050:intron | 30.0% | |
| ! | TTTCTGAAAGACTTCAAACA+AGG | - | chr8_3:3420991-3421010 | Msa1270050:intron | 30.0% |
| TTATAGGCACAAAGATATGT+TGG | + | chr8_3:3421039-3421058 | None:intergenic | 30.0% | |
| ATGTATTAATTGCGTTTGTC+CGG | - | chr8_3:3421119-3421138 | Msa1270050:CDS | 30.0% | |
| ACAGATATGAATAGAAACAC+AGG | - | chr8_3:3421153-3421172 | Msa1270050:CDS | 30.0% | |
| !! | AGATTTGTCTTGCTTTTGAA+AGG | - | chr8_3:3421269-3421288 | Msa1270050:CDS | 30.0% |
| ATATGATAGGATATGTGTCT+TGG | - | chr8_3:3421316-3421335 | Msa1270050:intron | 30.0% | |
| TTCAGATATGCTGTTGTTAC+AGG | - | chr8_3:3418780-3418799 | Msa1270050:exon | 35.0% | |
| GTTTGGAAGACTTGATATCT+TGG | - | chr8_3:3419013-3419032 | Msa1270050:CDS | 35.0% | |
| CACCAGATTCACCAAATAAA+TGG | + | chr8_3:3419166-3419185 | None:intergenic | 35.0% | |
| ! | TTATTTGGTGAATCTGGTGA+AGG | - | chr8_3:3419167-3419186 | Msa1270050:intron | 35.0% |
| ! | CTTCAAATTAAAGGCGTGTT+TGG | - | chr8_3:3419638-3419657 | Msa1270050:intron | 35.0% |
| TCCAAAGTTTGTAACAGGTT+GGG | + | chr8_3:3419674-3419693 | None:intergenic | 35.0% | |
| ATCCAAAGTTTGTAACAGGT+TGG | + | chr8_3:3419675-3419694 | None:intergenic | 35.0% | |
| TACCTGCACATGAAAATTCA+AGG | + | chr8_3:3419779-3419798 | None:intergenic | 35.0% | |
| ACTGAGTCAAACTTATGAGA+TGG | - | chr8_3:3419967-3419986 | Msa1270050:CDS | 35.0% | |
| GAATCAGATAACTGAAGAAG+AGG | + | chr8_3:3420052-3420071 | None:intergenic | 35.0% | |
| GTTAATGTTTCTTCTACTGC+AGG | - | chr8_3:3420082-3420101 | Msa1270050:CDS | 35.0% | |
| TTCTACTGCAGGACTATTAA+AGG | - | chr8_3:3420093-3420112 | Msa1270050:CDS | 35.0% | |
| CCTCAAAAGTCTCAAATATC+AGG | + | chr8_3:3420318-3420337 | None:intergenic | 35.0% | |
| !! | CCTGATATTTGAGACTTTTG+AGG | - | chr8_3:3420315-3420334 | Msa1270050:intron | 35.0% |
| !! | GAGACTTTTGAGGACTAAAT+TGG | - | chr8_3:3420325-3420344 | Msa1270050:intron | 35.0% |
| ACAAGGTTCACTTGAAAACA+AGG | - | chr8_3:3421008-3421027 | Msa1270050:intron | 35.0% | |
| CAAGGTTCACTTGAAAACAA+GGG | - | chr8_3:3421009-3421028 | Msa1270050:intron | 35.0% | |
| !!! | AGCTGCTTTTGAGAGTTTAT+AGG | + | chr8_3:3421055-3421074 | None:intergenic | 35.0% |
| TGTATTAATTGCGTTTGTCC+GGG | - | chr8_3:3421120-3421139 | Msa1270050:CDS | 35.0% | |
| TTAAAATGGGGGGATATGAT+AGG | - | chr8_3:3421303-3421322 | Msa1270050:intron | 35.0% | |
| ! | TTTGTAACAGGTTGGGACAA+CGG | + | chr8_3:3419667-3419686 | None:intergenic | 40.0% |
| TCCCAACCTGTTACAAACTT+TGG | - | chr8_3:3419670-3419689 | Msa1270050:intron | 40.0% | |
| ! | AGCCTTGAATTTTCATGTGC+AGG | - | chr8_3:3419774-3419793 | Msa1270050:intron | 40.0% |
| CATGTGCAGGTAAATAATGC+AGG | - | chr8_3:3419787-3419806 | Msa1270050:intron | 40.0% | |
| TGGTTTCAACACAGACGATA+TGG | - | chr8_3:3419816-3419835 | Msa1270050:intron | 40.0% | |
| TGGTGGTAAAGAAACTACTG+AGG | - | chr8_3:3420018-3420037 | Msa1270050:CDS | 40.0% | |
| CTGCAGGACTATTAAAGGTA+AGG | - | chr8_3:3420098-3420117 | Msa1270050:CDS | 40.0% | |
| ! | GTGTTTTCACCTATGAAGCA+TGG | - | chr8_3:3420404-3420423 | Msa1270050:intron | 40.0% |
| TCACCTATGAAGCATGGATA+CGG | - | chr8_3:3420410-3420429 | Msa1270050:intron | 40.0% | |
| GCACGAACACTTATCAGATT+AGG | + | chr8_3:3420545-3420564 | None:intergenic | 40.0% | |
| ! | ACATATCAAACGAATGGGCT+AGG | - | chr8_3:3420908-3420927 | Msa1270050:intron | 40.0% |
| ! | TACCTCGTCTATTAGCTCTT+CGG | + | chr8_3:3420962-3420981 | None:intergenic | 40.0% |
| GTTCACTTGAAAACAAGGGT+TGG | - | chr8_3:3421013-3421032 | Msa1270050:intron | 40.0% | |
| ! | ATAAGCTAGAAGCCTTGTGT+AGG | + | chr8_3:3421085-3421104 | None:intergenic | 40.0% |
| GATAGGATATGTGTCTTGGA+TGG | - | chr8_3:3421320-3421339 | Msa1270050:intron | 40.0% | |
| TGTCCGTATCCATGCTTCAT+AGG | + | chr8_3:3420416-3420435 | None:intergenic | 45.0% | |
| ATGAAGCATGGATACGGACA+CGG | - | chr8_3:3420416-3420435 | Msa1270050:intron | 45.0% | |
| TTATCAGATTAGGCGTGTCC+AGG | + | chr8_3:3420535-3420554 | None:intergenic | 45.0% | |
| ATCCGAAGAGCTAATAGACG+AGG | - | chr8_3:3420957-3420976 | Msa1270050:intron | 45.0% | |
| !! | GCTAATAGACGAGGTACTGA+AGG | - | chr8_3:3420966-3420985 | Msa1270050:intron | 45.0% |
| CAGCTGTGAATTCCTACACA+AGG | - | chr8_3:3421070-3421089 | Msa1270050:CDS | 45.0% | |
| TCGAGTGATTCCACCACTTC+TGG | - | chr8_3:3421228-3421247 | Msa1270050:CDS | 50.0% | |
| !! | GAAGAAGTGTCCAGAAGTGG+TGG | + | chr8_3:3421241-3421260 | None:intergenic | 50.0% |
| !! | AGCGAAGAAGTGTCCAGAAG+TGG | + | chr8_3:3421244-3421263 | None:intergenic | 50.0% |
| TGTTGGCGTCTCAGTGTGTC+TGG | + | chr8_3:3420445-3420464 | None:intergenic | 55.0% | |
| GATTAGGCGTGTCCAGGTGT+CGG | + | chr8_3:3420529-3420548 | None:intergenic | 55.0% | |
| CACGTGTCGTGTCCGACACC+TGG | - | chr8_3:3420514-3420533 | Msa1270050:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_3 | gene | 3418627 | 3421448 | 3418627 | ID=Msa1270050;Name=Msa1270050 |
| chr8_3 | mRNA | 3418627 | 3421448 | 3418627 | ID=Msa1270050-mRNA-1;Parent=Msa1270050;Name=Msa1270050-mRNA-1;_AED=0.25;_eAED=0.25;_QI=46|1|1|1|1|1|5|161|288 |
| chr8_3 | exon | 3418627 | 3419168 | 3418627 | ID=Msa1270050-mRNA-1:exon:1103;Parent=Msa1270050-mRNA-1 |
| chr8_3 | exon | 3419961 | 3420134 | 3419961 | ID=Msa1270050-mRNA-1:exon:1102;Parent=Msa1270050-mRNA-1 |
| chr8_3 | exon | 3420238 | 3420279 | 3420238 | ID=Msa1270050-mRNA-1:exon:1101;Parent=Msa1270050-mRNA-1 |
| chr8_3 | exon | 3421041 | 3421290 | 3421041 | ID=Msa1270050-mRNA-1:exon:1100;Parent=Msa1270050-mRNA-1 |
| chr8_3 | exon | 3421383 | 3421448 | 3421383 | ID=Msa1270050-mRNA-1:exon:1099;Parent=Msa1270050-mRNA-1 |
| chr8_3 | five_prime_UTR | 3421403 | 3421448 | 3421403 | ID=Msa1270050-mRNA-1:five_prime_utr;Parent=Msa1270050-mRNA-1 |
| chr8_3 | CDS | 3421383 | 3421402 | 3421383 | ID=Msa1270050-mRNA-1:cds;Parent=Msa1270050-mRNA-1 |
| chr8_3 | CDS | 3421041 | 3421290 | 3421041 | ID=Msa1270050-mRNA-1:cds;Parent=Msa1270050-mRNA-1 |
| chr8_3 | CDS | 3420238 | 3420279 | 3420238 | ID=Msa1270050-mRNA-1:cds;Parent=Msa1270050-mRNA-1 |
| chr8_3 | CDS | 3419961 | 3420134 | 3419961 | ID=Msa1270050-mRNA-1:cds;Parent=Msa1270050-mRNA-1 |
| chr8_3 | CDS | 3418788 | 3419168 | 3418788 | ID=Msa1270050-mRNA-1:cds;Parent=Msa1270050-mRNA-1 |
| chr8_3 | three_prime_UTR | 3418627 | 3418787 | 3418627 | ID=Msa1270050-mRNA-1:three_prime_utr;Parent=Msa1270050-mRNA-1 |
| Gene Sequence |
| Protein sequence |
