AlfalfaGEDB | Msa1270050

M. sativa cultivar zhongmu-4 / Msa1270050

KEGG: K15095
GO: -
PFAM: PF00106.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1270050	XP_003627594.1	97.222	288	7	1	1	288	1	287	0.0	575

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1270050	sp\|Q9M2E2\|SDR1_ARATH	57.095	296	119	3	1	288	1	296	2.83e-116	339

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1270050	G7LBT2	97.222	288	7	1	1	288	1	287	0.0	575

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0001830	Msa1270050	0.883230	5.348766e-71	-8.615850e-47
Msa0090380	Msa1270050	0.806490	8.021312e-50	-8.615850e-47
Msa0105410	Msa1270050	0.807945	3.944613e-50	-8.615850e-47
Msa0190930	Msa1270050	0.809130	2.203482e-50	-8.615850e-47
Msa0233250	Msa1270050	0.820407	6.971946e-53	-8.615850e-47
Msa0233970	Msa1270050	0.817034	4.064863e-52	-8.615850e-47
Msa0272400	Msa1270050	0.828059	1.109611e-54	-8.615850e-47
Msa0311500	Msa1270050	0.823015	1.738748e-53	-8.615850e-47
Msa0311510	Msa1270050	0.812818	3.501711e-51	-8.615850e-47
Msa0312590	Msa1270050	0.823104	1.657908e-53	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1270050	MtrunA17_Chr8g0349741	97.222	288	7	1	1	288	1	287	0.0	575
Msa1270050	MtrunA17_Chr7g0247011	66.319	288	95	2	1	288	1	286	1.05e-138	392
Msa1270050	MtrunA17_Chr7g0247031	64.211	285	97	4	6	288	7	288	3.68e-127	363
Msa1270050	MtrunA17_Chr8g0349781	58.667	300	110	4	1	288	1	298	4.66e-122	351
Msa1270050	MtrunA17_Chr8g0349811	57.000	300	115	4	1	288	1	298	9.58e-119	342
Msa1270050	MtrunA17_Chr8g0349851	57.432	296	111	4	6	288	6	299	1.49e-118	342
Msa1270050	MtrunA17_Chr8g0349801	56.000	300	118	4	1	288	1	298	7.77e-116	335
Msa1270050	MtrunA17_Chr8g0349761	59.794	291	103	5	7	285	8	296	2.15e-115	334
Msa1270050	MtrunA17_Chr8g0349771	56.667	300	116	4	1	288	1	298	1.34e-114	332
Msa1270050	MtrunA17_Chr7g0247041	57.586	290	114	4	7	288	6	294	1.94e-113	328

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1270050	AT3G61220.1	57.095	296	119	3	1	288	1	296	2.87e-117	339
Msa1270050	AT3G61220.2	57.586	290	115	3	7	288	14	303	4.70e-116	336
Msa1270050	AT3G61220.3	57.586	290	115	3	7	288	38	327	1.34e-115	336
Msa1270050	AT2G24190.1	54.027	298	125	4	1	288	1	296	5.61e-111	323
Msa1270050	AT2G24190.2	54.452	292	121	4	7	288	12	301	3.94e-110	321
Msa1270050	AT1G01800.1	54.730	296	123	4	3	288	1	295	4.24e-107	313
Msa1270050	AT1G01800.2	54.106	207	85	3	91	288	55	260	3.37e-69	215
Msa1270050	AT5G51030.1	40.541	296	146	10	4	286	35	313	5.39e-60	193
Msa1270050	AT3G59710.3	41.489	282	143	7	4	280	16	280	1.06e-58	189
Msa1270050	AT3G59710.1	41.489	282	143	7	4	280	26	290	1.45e-58	189

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Find 58 sgRNAs with CRISPR-Local

Find 143 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATAAACAGCAATGAACAAA+TGG	0.385084	8_3:+3418690	None:intergenic
ACAAGGTTCACTTGAAAACA+AGG	0.549206	8_3:-3419045	Msa1270050:CDS
ACAGATATGAATAGAAACAC+AGG	0.645826	8_3:-3418900	Msa1270050:CDS
ACAGGATCAAATAAAGGTAT+AGG	0.380633	8_3:-3421255	Msa1270050:CDS
ACATATCAAACGAATGGGCT+AGG	0.522049	8_3:-3419145	Msa1270050:CDS
ACCAATGAAGCAATACTAAA+AGG	0.304929	8_3:+3421081	None:intergenic
ACGCAATTAATACATAAGTT+TGG	0.345471	8_3:+3418942	None:intergenic
ACTGAGTCAAACTTATGAGA+TGG	0.614913	8_3:-3420086	Msa1270050:CDS
ACTTCTAGAAATCAGAAAAG+AGG	0.511868	8_3:-3421183	Msa1270050:CDS
AGCGAAGAAGTGTCCAGAAG+TGG	0.605903	8_3:+3418812	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CTATGGCAATTTCAAAAGAA+AGG	-	chr8_3:3418671-3418690	Msa1270050:three_prime_UTR	30.0%
GTTGTTACAGGATCAAATAA+AGG	-	chr8_3:3418792-3418811	Msa1270050:CDS	30.0%
ACCAATGAAGCAATACTAAA+AGG	+	chr8_3:3418975-3418994	None:intergenic	30.0%
TTCTTGATTCTCATTCTCAT+AGG	-	chr8_3:3419277-3419296	Msa1270050:intron	30.0%
TGATAAAAGCTCTTAGCAAA+AGG	+	chr8_3:3419332-3419351	None:intergenic	30.0%
GCACAAACACTTCAAATTAA+AGG	-	chr8_3:3419629-3419648	Msa1270050:intron	30.0%
GTTTATCCAAAGTTTGTAAC+AGG	+	chr8_3:3419679-3419698	None:intergenic	30.0%
GTAAATAATGCAGGAATCAA+TGG	-	chr8_3:3419796-3419815	Msa1270050:intron	30.0%
AGTTCCCTCCAATTAATAAT+TGG	+	chr8_3:3419950-3419969	None:intergenic	30.0%
GAAGAAACATTAACAATCAC+AGG	+	chr8_3:3420076-3420095	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	3418627	3421448	3418627	ID=Msa1270050;Name=Msa1270050
chr8_3	mRNA	3418627	3421448	3418627	ID=Msa1270050-mRNA-1;Parent=Msa1270050;Name=Msa1270050-mRNA-1;_AED=0.25;_eAED=0.25;_QI=46\|1\|1\|1\|1\|1\|5\|161\|288
chr8_3	exon	3418627	3419168	3418627	ID=Msa1270050-mRNA-1:exon:1103;Parent=Msa1270050-mRNA-1
chr8_3	exon	3419961	3420134	3419961	ID=Msa1270050-mRNA-1:exon:1102;Parent=Msa1270050-mRNA-1
chr8_3	exon	3420238	3420279	3420238	ID=Msa1270050-mRNA-1:exon:1101;Parent=Msa1270050-mRNA-1
chr8_3	exon	3421041	3421290	3421041	ID=Msa1270050-mRNA-1:exon:1100;Parent=Msa1270050-mRNA-1
chr8_3	exon	3421383	3421448	3421383	ID=Msa1270050-mRNA-1:exon:1099;Parent=Msa1270050-mRNA-1
chr8_3	five_prime_UTR	3421403	3421448	3421403	ID=Msa1270050-mRNA-1:five_prime_utr;Parent=Msa1270050-mRNA-1
chr8_3	CDS	3421383	3421402	3421383	ID=Msa1270050-mRNA-1:cds;Parent=Msa1270050-mRNA-1
chr8_3	CDS	3421041	3421290	3421041	ID=Msa1270050-mRNA-1:cds;Parent=Msa1270050-mRNA-1
chr8_3	CDS	3420238	3420279	3420238	ID=Msa1270050-mRNA-1:cds;Parent=Msa1270050-mRNA-1
chr8_3	CDS	3419961	3420134	3419961	ID=Msa1270050-mRNA-1:cds;Parent=Msa1270050-mRNA-1
chr8_3	CDS	3418788	3419168	3418788	ID=Msa1270050-mRNA-1:cds;Parent=Msa1270050-mRNA-1
chr8_3	three_prime_UTR	3418627	3418787	3418627	ID=Msa1270050-mRNA-1:three_prime_utr;Parent=Msa1270050-mRNA-1

Gene Sequence

>Msa1270050

ATGGCAATTTCAAAAGAAAGATATGCTGTTGTTACAGGATCAAATAAAGGTATAGGATTGGAAACTGTTAAAAGATTGGCTTCAAATGGAATTAAAGTTGTTCTTACTTCTAGAAATCAGAAAAGAGGGATTCAAGCTTTTGAGAAATTGAAGAAAGAATTTGAATTTTGTAATCTTGTTGTTTTTCATCAACTTGATGTTACTGATCCTTTTAGTATTGCTTCATTGGTTGAATTTGTTAAGACTCAGTTTGGAAGACTTGATATCTTGGTAAATAATGCAGGAATCAATGGTTTCAACACAGACGATATGGTTGAACCAATTATTAATTGGAGGGAACTGAGTCAAACTTATGAGATGGCTGAAAATTGCATAATAACAAATTACTATGGTGGTAAAGAAACTACTGAGGCTTTTCTTCCTCTTCTTCAGTTATCTGATTCACCTGTGATTGTTAATGTTTCTTCTACTGCAGGACTATTAAAGTACATATCAAACGAATGGGCTAGGAGTGTGTTAGACGATACTGAAAATCTATCCGAAGAGCTAATAGACGAGGTACTGAAGGAGTTTCTGAAAGACTTCAAACAAGGTTCACTTGAAAACAAGGGTTGGCCAACATATCTTTGTGCCTATAAACTCTCAAAAGCAGCTGTGAATTCCTACACAAGGCTTCTAGCTTATAGACATCCAAACTTATGTATTAATTGCGTTTGTCCGGGTTTTGTTAAAACAGATATGAATAGAAACACAGGTATATTATCTGTTGAAAATGGTGCTGCTAGTGTTGTCAGATTAGCATTACTTTCGAGTGATTCCACCACTTCTGGACACTTCTTCGCTCGTCAAGATTTGTCTTGCTTTTGA

Protein sequence

>Msa1270050

MAISKERYAVVTGSNKGIGLETVKRLASNGIKVVLTSRNQKRGIQAFEKLKKEFEFCNLVVFHQLDVTDPFSIASLVEFVKTQFGRLDILVNNAGINGFNTDDMVEPIINWRELSQTYEMAENCIITNYYGGKETTEAFLPLLQLSDSPVIVNVSSTAGLLKYISNEWARSVLDDTENLSEELIDEVLKEFLKDFKQGSLENKGWPTYLCAYKLSKAAVNSYTRLLAYRHPNLCINCVCPGFVKTDMNRNTGILSVENGAASVVRLALLSSDSTTSGHFFARQDLSCF*