Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0483660 | AES86526.1 | 96.842 | 95 | 3 | 0 | 1 | 95 | 1 | 95 | 9.74e-57 | 194 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0483660 | sp|P0C897|Y3264_ARATH | 50.633 | 79 | 38 | 1 | 16 | 93 | 16 | 94 | 1.13e-19 | 85.5 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0483660 | G7JFR7 | 96.842 | 95 | 3 | 0 | 1 | 95 | 1 | 95 | 4.65e-57 | 194 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0483650 | Msa0483660 | 0.871006 | 9.492798e-67 | -8.615850e-47 |
| Msa0483660 | Msa0586910 | 0.888246 | 6.998530e-73 | -8.615850e-47 |
| Msa0483660 | Msa0626720 | 0.809793 | 1.587134e-50 | -8.615850e-47 |
| Msa0483660 | Msa0664850 | 0.883705 | 3.579003e-71 | -8.615850e-47 |
| Msa0483660 | Msa0868020 | 0.888827 | 4.180506e-73 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0483660 | MtrunA17_Chr4g0002911 | 96.842 | 95 | 3 | 0 | 1 | 95 | 1 | 95 | 1.03e-60 | 194 |
| Msa0483660 | MtrunA17_Chr4g0002901 | 81.053 | 95 | 18 | 0 | 1 | 95 | 1 | 95 | 1.51e-45 | 154 |
| Msa0483660 | MtrunA17_Chr4g0002861 | 84.211 | 95 | 15 | 0 | 1 | 95 | 1 | 95 | 1.17e-44 | 151 |
| Msa0483660 | MtrunA17_Chr7g0220521 | 61.053 | 95 | 36 | 1 | 1 | 95 | 1 | 94 | 1.29e-36 | 129 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0483660 | AT3G02645.1 | 50.633 | 79 | 38 | 1 | 16 | 93 | 16 | 94 | 1.15e-20 | 85.5 |
Find 18 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTCCTCAAACTCTTCTTCT+AGG | 0.153796 | 3_4:+342502 | None:intergenic |
| CAACCTTTGATGAGAATCTT+TGG | 0.179806 | 3_4:-342545 | Msa0483660:CDS |
| TGTTGTGGAACATAAGAATT+TGG | 0.183132 | 3_4:+342420 | None:intergenic |
| AAGCTTTGGCACATTGAAAA+TGG | 0.249135 | 3_4:+342458 | None:intergenic |
| CAATGTGCCAAAGCTTCTTA+TGG | 0.260296 | 3_4:-342451 | Msa0483660:CDS |
| CAATTGGTCCCTACCATTAT+TGG | 0.314543 | 3_4:-342392 | Msa0483660:CDS |
| GAAGAGTTTGAGGAGGAAAA+TGG | 0.326776 | 3_4:-342495 | Msa0483660:CDS |
| AACCTTTGATGAGAATCTTT+GGG | 0.378384 | 3_4:-342544 | Msa0483660:CDS |
| CTCCTCAAACTCTTCTTCTA+GGG | 0.397644 | 3_4:+342503 | None:intergenic |
| AGAGTTCTTGACGCCAATAA+TGG | 0.405296 | 3_4:+342379 | None:intergenic |
| TCACTAGCCATAAGAAGCTT+TGG | 0.455256 | 3_4:+342444 | None:intergenic |
| GTTCCACAACAAGTTGCAAT+TGG | 0.462687 | 3_4:-342408 | Msa0483660:CDS |
| AACCCTAGAAGAAGAGTTTG+AGG | 0.465593 | 3_4:-342505 | Msa0483660:CDS |
| GGACCAATTGCAACTTGTTG+TGG | 0.498583 | 3_4:+342405 | None:intergenic |
| CACCCAAAGATTCTCATCAA+AGG | 0.543342 | 3_4:+342542 | None:intergenic |
| TTCTTGACGCCAATAATGGT+AGG | 0.599841 | 3_4:+342383 | None:intergenic |
| CCTAGAAGAAGAGTTTGAGG+AGG | 0.616622 | 3_4:-342502 | Msa0483660:CDS |
| TCTTGACGCCAATAATGGTA+GGG | 0.634295 | 3_4:+342384 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGTTTTAGAATTATATGACA+TGG | + | chr3_4:342330-342349 | None:intergenic | 20.0% |
| AACCTTTGATGAGAATCTTT+GGG | - | chr3_4:342349-342368 | Msa0483660:CDS | 30.0% | |
| TGTTGTGGAACATAAGAATT+TGG | + | chr3_4:342476-342495 | None:intergenic | 30.0% | |
| !! | CTTGACATTGTAGTTGTTTT+TGG | + | chr3_4:342580-342599 | None:intergenic | 30.0% |
| CAACCTTTGATGAGAATCTT+TGG | - | chr3_4:342348-342367 | Msa0483660:CDS | 35.0% | |
| ! | AAGCTTTGGCACATTGAAAA+TGG | + | chr3_4:342438-342457 | None:intergenic | 35.0% |
| CACCCAAAGATTCTCATCAA+AGG | + | chr3_4:342354-342373 | None:intergenic | 40.0% | |
| AACCCTAGAAGAAGAGTTTG+AGG | - | chr3_4:342388-342407 | Msa0483660:CDS | 40.0% | |
| CTCCTCAAACTCTTCTTCTA+GGG | + | chr3_4:342393-342412 | None:intergenic | 40.0% | |
| GAAGAGTTTGAGGAGGAAAA+TGG | - | chr3_4:342398-342417 | Msa0483660:CDS | 40.0% | |
| CAATGTGCCAAAGCTTCTTA+TGG | - | chr3_4:342442-342461 | Msa0483660:CDS | 40.0% | |
| TCACTAGCCATAAGAAGCTT+TGG | + | chr3_4:342452-342471 | None:intergenic | 40.0% | |
| GTTCCACAACAAGTTGCAAT+TGG | - | chr3_4:342485-342504 | Msa0483660:CDS | 40.0% | |
| CAATTGGTCCCTACCATTAT+TGG | - | chr3_4:342501-342520 | Msa0483660:CDS | 40.0% | |
| TCTTGACGCCAATAATGGTA+GGG | + | chr3_4:342512-342531 | None:intergenic | 40.0% | |
| TTCTTGACGCCAATAATGGT+AGG | + | chr3_4:342513-342532 | None:intergenic | 40.0% | |
| AGAGTTCTTGACGCCAATAA+TGG | + | chr3_4:342517-342536 | None:intergenic | 40.0% | |
| CCTCCTCAAACTCTTCTTCT+AGG | + | chr3_4:342394-342413 | None:intergenic | 45.0% | |
| CCTAGAAGAAGAGTTTGAGG+AGG | - | chr3_4:342391-342410 | Msa0483660:CDS | 45.0% | |
| !! | GGACCAATTGCAACTTGTTG+TGG | + | chr3_4:342491-342510 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_4 | gene | 342308 | 342607 | 342308 | ID=Msa0483660;Name=Msa0483660 |
| chr3_4 | mRNA | 342308 | 342607 | 342308 | ID=Msa0483660-mRNA-1;Parent=Msa0483660;Name=Msa0483660-mRNA-1;_AED=0.41;_eAED=0.43;_QI=0|-1|0|1|-1|1|1|0|99 |
| chr3_4 | exon | 342308 | 342607 | 342308 | ID=Msa0483660-mRNA-1:exon:93;Parent=Msa0483660-mRNA-1 |
| chr3_4 | CDS | 342308 | 342607 | 342308 | ID=Msa0483660-mRNA-1:cds;Parent=Msa0483660-mRNA-1 |
| Gene Sequence |
| Protein sequence |
