AlfalfaGEDB | Msa0522520

M. sativa cultivar zhongmu-4 / Msa0522520

KEGG: -
GO: -
PFAM: PF17123.8

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0522520	XP_003603694.3	95.490	776	22	7	1	771	1	768	0.0	1509

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0522520	sp\|Q9LTA6\|WAV3_ARATH	55.472	795	273	28	3	771	1	740	0.0	729

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0522520	G7J445	95.478	774	22	7	3	771	1	766	0.0	1505

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0136890	Msa0522520	0.807279	5.462152e-50	-8.615850e-47
Msa0434640	Msa0522520	0.843239	1.583267e-58	-8.615850e-47
Msa0477880	Msa0522520	0.804088	2.554908e-49	-8.615850e-47
Msa0478410	Msa0522520	0.847997	8.113744e-60	-8.615850e-47
Msa0522520	Msa1068540	0.807432	5.070430e-50	-8.615850e-47
Msa0522520	Msa1433100	0.805320	1.413038e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0522520	MtrunA17_Chr3g0141061	95.419	764	22	7	1	759	1	756	0.0	1484
Msa0522520	MtrunA17_Chr5g0411491	44.076	785	329	24	3	771	1	691	2.81e-177	525
Msa0522520	MtrunA17_Chr8g0366871	34.264	788	322	22	3	771	1	611	1.03e-114	360
Msa0522520	MtrunA17_Chr2g0326911	31.571	681	346	19	130	756	68	682	1.35e-88	294
Msa0522520	MtrunA17_Chr1g0199601	32.562	648	339	19	109	707	54	652	1.01e-86	289
Msa0522520	MtrunA17_Chr4g0028831	32.528	621	317	21	130	707	69	630	1.07e-80	273
Msa0522520	MtrunA17_Chr2g0290981	29.150	494	264	18	116	593	78	501	1.76e-36	145

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0522520	AT5G49665.1	55.472	795	273	28	3	771	1	740	0.0	729
Msa0522520	AT2G22680.1	41.677	799	320	28	3	771	1	683	1.49e-162	487
Msa0522520	AT4G37890.2	41.519	790	362	22	3	771	1	711	4.20e-155	468
Msa0522520	AT4G37890.1	39.976	818	363	24	3	771	1	739	1.24e-152	463
Msa0522520	AT5G65683.1	38.119	808	370	29	3	771	1	717	4.21e-127	396
Msa0522520	AT2G38970.1	34.105	648	335	21	94	707	32	621	1.39e-95	312
Msa0522520	AT5G60710.1	34.566	622	337	16	109	707	48	622	1.10e-87	291
Msa0522520	AT3G54780.1	33.893	596	293	19	132	701	73	593	1.14e-87	291
Msa0522520	AT3G54780.3	32.759	580	282	17	130	701	72	551	1.91e-82	276
Msa0522520	AT3G54780.2	32.872	578	280	17	132	701	73	550	2.68e-82	275

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Find 227 sgRNAs with CRISPR-Local

Find 263 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAATTGGGGATGGGAA+TGG	0.515689	3_4:-75719024	Msa0522520:three_prime_UTR
AAAAGGGTGCGAAAATATGG+TGG	0.598978	3_4:-75719282	Msa0522520:CDS
AAACTGGTTGAGATTTAGAT+TGG	0.410433	3_4:+75721360	None:intergenic
AAAGAACTTATTATAATTAG+AGG	0.284006	3_4:-75719050	Msa0522520:three_prime_UTR
AAAGCACCACCGCCACTCCG+CGG	0.698878	3_4:-75720451	Msa0522520:CDS
AAAGGAGAGCGAGGAGAAGT+TGG	0.431736	3_4:+75721203	None:intergenic
AAAGGTGACGCAGAAGACAA+AGG	0.649534	3_4:+75721668	None:intergenic
AAATTCCGGTTCATGCGTTT+GGG	0.366480	3_4:-75719976	Msa0522520:CDS
AAATTCTCCCATTTAAAGGT+GGG	0.565551	3_4:+75718933	None:intergenic
AACACACGAAACATACGCTT+TGG	0.429505	3_4:-75720485	Msa0522520:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAAGAAAGAATGGATCTAAA+AGG	+	chr3_4:75719094-75719113	None:intergenic	30.0%
GTTGTTCTTATTATCTGGTA+TGG	+	chr3_4:75719266-75719285	None:intergenic	30.0%
AAACTGGTTGAGATTTAGAT+TGG	+	chr3_4:75719384-75719403	None:intergenic	30.0%
TCAAAAACAGCTTCAAATTC+AGG	-	chr3_4:75719575-75719594	Msa0522520:CDS	30.0%
CAAAAACAGCTTCAAATTCA+GGG	-	chr3_4:75719576-75719595	Msa0522520:CDS	30.0%
AATAAAGTCAGTGACTTACA+TGG	-	chr3_4:75721568-75721587	Msa0522520:five_prime_UTR	30.0%
TTAGAGGAAGAAAACAATTG+GGG	-	chr3_4:75721707-75721726	Msa0522520:five_prime_UTR	30.0%
ATCATCTTCTAATGCACAAA+GGG	+	chr3_4:75721769-75721788	None:intergenic	30.0%
AATCATCTTCTAATGCACAA+AGG	+	chr3_4:75721770-75721789	None:intergenic	30.0%
AAATTCTCCCATTTAAAGGT+GGG	+	chr3_4:75721811-75721830	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_4	gene	75718832	75721931	75718832	ID=Msa0522520;Name=Msa0522520
chr3_4	mRNA	75718832	75721931	75718832	ID=Msa0522520-mRNA-1;Parent=Msa0522520;Name=Msa0522520-mRNA-1;_AED=0.00;_eAED=0.00;_QI=376\|1\|1\|1\|1\|1\|2\|319\|771
chr3_4	exon	75718832	75721076	75718832	ID=Msa0522520-mRNA-1:exon:21725;Parent=Msa0522520-mRNA-1
chr3_4	exon	75721166	75721931	75721166	ID=Msa0522520-mRNA-1:exon:21724;Parent=Msa0522520-mRNA-1
chr3_4	five_prime_UTR	75721556	75721931	75721556	ID=Msa0522520-mRNA-1:five_prime_utr;Parent=Msa0522520-mRNA-1
chr3_4	CDS	75721166	75721555	75721166	ID=Msa0522520-mRNA-1:cds;Parent=Msa0522520-mRNA-1
chr3_4	CDS	75719151	75721076	75719151	ID=Msa0522520-mRNA-1:cds;Parent=Msa0522520-mRNA-1
chr3_4	three_prime_UTR	75718832	75719150	75718832	ID=Msa0522520-mRNA-1:three_prime_utr;Parent=Msa0522520-mRNA-1

Gene Sequence

>Msa0522520

ATGAAAATGGGTACTGGGTGGAGAAGAGCCTTTTGTACACGAGACCCTGAATCTACCATACCAGATAATAAGAACAACAACAACAACGGAAGTCCTAATCCTAGTCCAAGAAGCTGTGCTAGATTGAGTTTTCTTTCTGGTGGTAGTAGTAATCCTTCAACTCCACGTTTGCACCAATCTAAATCTCAACCAGTTTCTAGTCCAAGTCTTCGTTGTCGAACAATAACTGAAGCAGCTTCTCAAATTACAAATGATAGCCCCAGATTTCAAAGTAGCAAAAGTAGCACCCCAAGATCAACAAAGTCTCCTAGAGTTAACTCAATATCAAATCCAACTTCTCCTCGCTCTCCTTTGAAGTTGTCCCTCTTCAAAAACAGCTTCAAATTCAGGAGTAGCTGTGGAATCTGTTTGAACAGCGTGAAAACGGGGCAGGGGAAGGCAATATACACAGCAGAATGTGCACATGCTTTTCATTTCCCTTGCATAGCAGCACATGTACGCAACCATGCTACCCTTGTTTGCCCTGTCTGCAACGCCACATGGAAAGACGTTCCTCTTCTAGCGGCGCACAAGAATCTCTCTTCTTCACAAGAAATTCCCAATGCAATTCCAAACCACAAAATACCTAATCCCTCTCCTGTTATCAGAACCAAGAACGTGGATCAGTCTCAACAAACTAATCATTCTGAATCAATAAGATCCTACGATGATGACGAGCCTCTACTCCTTTCTCCAACTTCTGGTGGTGGAAGGATTAACACTATCCCCGAAGCAGATGAAAATGCAGAGGAAGAAGATGATGACAACTGCGAGTTTCAAGGATTTTTCGTCAACGCAAAACCTACTTCCACCGCTAACAAAACGTACTCCGATGACATTCAAACCAGCGATGGAGTCGGTGATTCAAGAACTGTTCAGGTGAAGTTAATGCCAGAATGTGCCATCGTATCAGTTTCCAGAACACACGAAACATACGCTTTGGTTTTAAAAGTAAAAGCACCACCGCCACTCCGCGGCGGAACCAGCACACTTGATCCTTCACGGCGAGCTCCGATAGATCTTGTCACTGTTCTCGATGTTGGTGGAAGCATGTCCAGCGCAAAGCTTCACATGCTAAAACGCGCGATGCGTTTGGTTATTTCATCTCTTGGTCCTTCTGATAGGCTTTCAATTGTTGCTTTTTCGTCTGTTTCCAAACGGCTCTTACCGTTGAGAAGAATGACGGCTCAGGGTCAACGTTTGGCTCGGCGTATCGTTGACCGGCTTGTTACCGGTGAAGGTAACAGTGTCAGTGAAGCTTTGAGAAAAGCAACTACAGTTTTGGAGGATCGTAGAGAAAGAAACCCGGTTGCTAGCGTCATGCTGTTATCCGACGGTCAAGATGAAAAGGTTAATAACAGTAAGAATCAAAATCAAAATCAACGGAAGATGTACAACCACGCTTCTTCAACCCGGTTCGCTCATATTGAAATTCCGGTTCATGCGTTTGGGTTTGGTAGCAAAAGCGGTTATAGTCATGAACCGGGTGAGGATGCATTTGCAAAATGTGTTGGCGGTCTTTTGAGTGTTGTGGTTCAGGATTTAAGAGTTCAACTGGGCTTTCAATCTGATGATCCGGCCCGGGCCGCCGAGATCAGTGCTATATACTCTTGCAGTGGAAGACCCACGCTGTTGAGCTTAGGAGCCGTACGGCTCGGCGATTTATACGCTGAAGAAGAAAGAGAGCTTTTGGTGGAGATGAGAGTTCCAGCGTCAGCTTTAGGGTACGGGTCCCACCATGTGATGACGGTAAGGTGTCTTTATAAAGACCCCGCAAGTCAAGAGATAGTATACGGCAGAGAACAGGGACTTACAGTGCAACTACCTCAGAATCAATCTCAGAATATACGATCAACTGGGACAAGGATCGAAAGGTTGAGGAATCTTTTCATAACAACACGAGCCATAGCTGAGTCAAGGAGGCTTTTAGATCATAACAGTGACTTCACAAGTGCTCATCATTTACTTGCCTCGGCTCGTGCTCTTTTGATACAATCTGGCTCAACTTCAGCTGAGCAATATGTTCGTGGCTTGGAAGCTGAGCTGGCGGAGCTTCATTGGAGGAGACAGCAAGAACAGGTTCAAGTTGAGGCTCAGCAGCAACAAATGATGATGCAAAGAAGAAAAGGGTGCGAAAATATGGTGGATGAAAATGGTGAGCCACTTACACCTACTTCAGCTTGGAGAGCGGCTGAGAAGCTGGCTAGGATGGCTATGGTCAAGAAGTCTTTGAATAAAGTCAGTGACTTACATGGTTTCGAAAATGCTAGGTTTTGA

Protein sequence

>Msa0522520

MKMGTGWRRAFCTRDPESTIPDNKNNNNNGSPNPSPRSCARLSFLSGGSSNPSTPRLHQSKSQPVSSPSLRCRTITEAASQITNDSPRFQSSKSSTPRSTKSPRVNSISNPTSPRSPLKLSLFKNSFKFRSSCGICLNSVKTGQGKAIYTAECAHAFHFPCIAAHVRNHATLVCPVCNATWKDVPLLAAHKNLSSSQEIPNAIPNHKIPNPSPVIRTKNVDQSQQTNHSESIRSYDDDEPLLLSPTSGGGRINTIPEADENAEEEDDDNCEFQGFFVNAKPTSTANKTYSDDIQTSDGVGDSRTVQVKLMPECAIVSVSRTHETYALVLKVKAPPPLRGGTSTLDPSRRAPIDLVTVLDVGGSMSSAKLHMLKRAMRLVISSLGPSDRLSIVAFSSVSKRLLPLRRMTAQGQRLARRIVDRLVTGEGNSVSEALRKATTVLEDRRERNPVASVMLLSDGQDEKVNNSKNQNQNQRKMYNHASSTRFAHIEIPVHAFGFGSKSGYSHEPGEDAFAKCVGGLLSVVVQDLRVQLGFQSDDPARAAEISAIYSCSGRPTLLSLGAVRLGDLYAEEERELLVEMRVPASALGYGSHHVMTVRCLYKDPASQEIVYGREQGLTVQLPQNQSQNIRSTGTRIERLRNLFITTRAIAESRRLLDHNSDFTSAHHLLASARALLIQSGSTSAEQYVRGLEAELAELHWRRQQEQVQVEAQQQQMMMQRRKGCENMVDENGEPLTPTSAWRAAEKLARMAMVKKSLNKVSDLHGFENARF*