AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0522520

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0522520 MtrunA17_Chr3g0141061 95.419 764 22 7 1 759 1 756 0.0 1484
Msa0522520 MtrunA17_Chr5g0411491 44.076 785 329 24 3 771 1 691 2.81e-177 525
Msa0522520 MtrunA17_Chr8g0366871 34.264 788 322 22 3 771 1 611 1.03e-114 360
Msa0522520 MtrunA17_Chr2g0326911 31.571 681 346 19 130 756 68 682 1.35e-88 294
Msa0522520 MtrunA17_Chr1g0199601 32.562 648 339 19 109 707 54 652 1.01e-86 289
Msa0522520 MtrunA17_Chr4g0028831 32.528 621 317 21 130 707 69 630 1.07e-80 273
Msa0522520 MtrunA17_Chr2g0290981 29.150 494 264 18 116 593 78 501 1.76e-36 145
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0522520 AT5G49665.1 55.472 795 273 28 3 771 1 740 0.0 729
Msa0522520 AT2G22680.1 41.677 799 320 28 3 771 1 683 1.49e-162 487
Msa0522520 AT4G37890.2 41.519 790 362 22 3 771 1 711 4.20e-155 468
Msa0522520 AT4G37890.1 39.976 818 363 24 3 771 1 739 1.24e-152 463
Msa0522520 AT5G65683.1 38.119 808 370 29 3 771 1 717 4.21e-127 396
Msa0522520 AT2G38970.1 34.105 648 335 21 94 707 32 621 1.39e-95 312
Msa0522520 AT5G60710.1 34.566 622 337 16 109 707 48 622 1.10e-87 291
Msa0522520 AT3G54780.1 33.893 596 293 19 132 701 73 593 1.14e-87 291
Msa0522520 AT3G54780.3 32.759 580 282 17 130 701 72 551 1.91e-82 276
Msa0522520 AT3G54780.2 32.872 578 280 17 132 701 73 550 2.68e-82 275
Msa0522520 AT3G54780.4 32.759 580 282 17 130 701 121 600 6.36e-82 276
Msa0522520 AT3G54780.5 32.872 578 280 17 132 701 122 599 9.30e-82 275
Msa0522520 AT1G08050.1 30.808 594 291 17 133 701 69 567 3.92e-78 265

Find 227 sgRNAs with CRISPR-Local

Find 263 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCAAATTCTCCCATTTAA+AGG 0.203174 3_4:+75718929 None:intergenic
AAGACAAAGGAATTTAGTTT+AGG 0.233441 3_4:+75721681 None:intergenic
GTGTATGGCCCACCTTTAAA+TGG 0.258034 3_4:-75718941 Msa0522520:three_prime_UTR
CTCAATCTAGCACAGCTTCT+TGG 0.275682 3_4:+75721428 None:intergenic
AGCGTATGTTTCGTGTGTTC+TGG 0.277675 3_4:+75720489 None:intergenic
TAGGAGACTTTGTTGATCTT+GGG 0.283485 3_4:+75721246 None:intergenic
TTGAGACTGATCCACGTTCT+TGG 0.283557 3_4:+75720795 None:intergenic
AAAGAACTTATTATAATTAG+AGG 0.284006 3_4:-75719050 Msa0522520:three_prime_UTR
GAGAAAAGCAACTACAGTTT+TGG 0.284089 3_4:-75720143 Msa0522520:CDS
AGACAAAGGAATTTAGTTTA+GGG 0.294259 3_4:+75721682 None:intergenic
CATCTCTTGGTCCTTCTGAT+AGG 0.296073 3_4:-75720303 Msa0522520:CDS
CTACTCCTTTCTCCAACTTC+TGG 0.298621 3_4:-75720724 Msa0522520:CDS
AGAGTTCCAGCGTCAGCTTT+AGG 0.303683 3_4:-75719704 Msa0522520:CDS
TTATCTGGTATGGTAGATTC+AGG 0.306675 3_4:+75721488 None:intergenic
CGTTTGGTTATTTCATCTCT+TGG 0.311758 3_4:-75720316 Msa0522520:CDS
GAAATTCCGGTTCATGCGTT+TGG 0.312433 3_4:-75719977 Msa0522520:CDS
GAAGAAAGAATGGATCTAAA+AGG 0.313399 3_4:+75721650 None:intergenic
AAGAAAAGGGTGCGAAAATA+TGG 0.315590 3_4:-75719285 Msa0522520:CDS
CTAGGAGACTTTGTTGATCT+TGG 0.320258 3_4:+75721245 None:intergenic
TTGTTGTTGTTCTTATTATC+TGG 0.323779 3_4:+75721473 None:intergenic
TATACTATCTCTTGACTTGC+GGG 0.335850 3_4:+75719635 None:intergenic
CTGAGCTGGCGGAGCTTCAT+TGG 0.336931 3_4:-75719367 Msa0522520:CDS
GAGTTCCAGCGTCAGCTTTA+GGG 0.338033 3_4:-75719703 Msa0522520:CDS
ACGTTCTTGGTTCTGATAAC+AGG 0.345062 3_4:+75720808 None:intergenic
TTCCATGTGGCGTTGCAGAC+AGG 0.346447 3_4:+75720922 None:intergenic
ATCGTTGACCGGCTTGTTAC+CGG 0.348514 3_4:-75720193 Msa0522520:CDS
GATAGTATACGGCAGAGAAC+AGG 0.349583 3_4:-75719621 Msa0522520:CDS
TCAGGATTTAAGAGTTCAAC+TGG 0.352337 3_4:-75719870 Msa0522520:CDS
GTTCGCTCATATTGAAATTC+CGG 0.357741 3_4:-75719990 Msa0522520:CDS
CAGCTTCTTGGACTAGGATT+AGG 0.364630 3_4:+75721440 None:intergenic
AAATTCCGGTTCATGCGTTT+GGG 0.366480 3_4:-75719976 Msa0522520:CDS
TCTCAGAATATACGATCAAC+TGG 0.375463 3_4:-75719569 Msa0522520:CDS
AATTAGAGGAAGAAAACAAT+TGG 0.378258 3_4:-75719036 Msa0522520:three_prime_UTR
CCGGTTCATGCGTTTGGGTT+TGG 0.382763 3_4:-75719971 Msa0522520:CDS
ACTGATACGATGGCACATTC+TGG 0.382845 3_4:+75720514 None:intergenic
CGGTGATTCAAGAACTGTTC+AGG 0.383564 3_4:-75720548 Msa0522520:CDS
GCTAAAACGCGCGATGCGTT+TGG 0.384841 3_4:-75720332 Msa0522520:CDS
CTGTTTGAACAGCGTGAAAA+CGG 0.390254 3_4:-75721040 Msa0522520:CDS
TGAAGAAGAAAGAGAGCTTT+TGG 0.391859 3_4:-75719735 Msa0522520:CDS
TTTAAATGGGAGAATTTGAT+TGG 0.394069 3_4:-75718927 Msa0522520:three_prime_UTR
ATTAGAGGAAGAAAACAATT+GGG 0.396402 3_4:-75719035 Msa0522520:three_prime_UTR
ATGATGATGCAAAGAAGAAA+AGG 0.398008 3_4:-75719299 Msa0522520:CDS
CTGATAACAGGAGAGGGATT+AGG 0.400878 3_4:+75720820 None:intergenic
TGTTTGAACAGCGTGAAAAC+GGG 0.401641 3_4:-75721039 Msa0522520:CDS
AACAGCATGACGCTAGCAAC+CGG 0.405649 3_4:+75720100 None:intergenic
CGAAGACTTGGACTAGAAAC+TGG 0.406962 3_4:+75721344 None:intergenic
AAACTGGTTGAGATTTAGAT+TGG 0.410433 3_4:+75721360 None:intergenic
TTCTCAAAGTCTGAAACAAA+AGG 0.415025 3_4:-75721585 Msa0522520:five_prime_UTR
GAAAATATGGTGGATGAAAA+TGG 0.415794 3_4:-75719272 Msa0522520:CDS
GATGACAACTGCGAGTTTCA+AGG 0.418436 3_4:-75720646 Msa0522520:CDS
CATGGACTATGATCTTGAAA+AGG 0.419602 3_4:+75718830 None:intergenic
CTAGCACAGCTTCTTGGACT+AGG 0.420558 3_4:+75721434 None:intergenic
TGCTCATCATTTACTTGCCT+CGG 0.421096 3_4:-75719453 Msa0522520:CDS
CTCTCGAGGCGTTTAACTGC+AGG 0.422107 3_4:-75721859 Msa0522520:five_prime_UTR
AAGCGGTTATAGTCATGAAC+CGG 0.424642 3_4:-75719942 Msa0522520:CDS
TCGTGGCTTGGAAGCTGAGC+TGG 0.424913 3_4:-75719381 Msa0522520:CDS
CTGATCTCGGCGGCCCGGGC+CGG 0.425651 3_4:+75719830 None:intergenic
CTCCAACTTCTGGTGGTGGA+AGG 0.428818 3_4:-75720714 Msa0522520:CDS
AACACACGAAACATACGCTT+TGG 0.429505 3_4:-75720485 Msa0522520:CDS
AATGGGAGAATTTGATTGGT+TGG 0.430331 3_4:-75718923 Msa0522520:three_prime_UTR
AAAGGAGAGCGAGGAGAAGT+TGG 0.431736 3_4:+75721203 None:intergenic
GACGGCTCAGGGTCAACGTT+TGG 0.433573 3_4:-75720224 Msa0522520:CDS
TTCAATCTGATGATCCGGCC+CGG 0.444036 3_4:-75719844 Msa0522520:CDS
TCTCAACGGTAAGAGCCGTT+TGG 0.445243 3_4:+75720252 None:intergenic
CCAGCGTCAGCTTTAGGGTA+CGG 0.445543 3_4:-75719698 Msa0522520:CDS
GCAAGTCAAGAGATAGTATA+CGG 0.447444 3_4:-75719632 Msa0522520:CDS
CCAAACCCAAACGCATGAAC+CGG 0.450222 3_4:+75719971 None:intergenic
CTTTCTCCAACTTCTGGTGG+TGG 0.453090 3_4:-75720718 Msa0522520:CDS
GAATTTCAATATGAGCGAAC+CGG 0.453411 3_4:+75719993 None:intergenic
TTGGAGAGCGGCTGAGAAGC+TGG 0.453516 3_4:-75719225 Msa0522520:CDS
TTGTGGTTTGGAATTGCATT+GGG 0.453923 3_4:+75720847 None:intergenic
CAGCGTCAGCTTTAGGGTAC+GGG 0.456057 3_4:-75719697 Msa0522520:CDS
TGGTGCAAACGTGGAGTTGA+AGG 0.458578 3_4:+75721380 None:intergenic
GGAATTTAGTTTAGGGAGCA+TGG 0.462544 3_4:+75721689 None:intergenic
GTTCCGCCGCGGAGTGGCGG+TGG 0.462679 3_4:+75720445 None:intergenic
CGTTGCAGACAGGGCAAACA+AGG 0.468208 3_4:+75720932 None:intergenic
TCGTGAATGAAAATGGGTAC+TGG 0.469774 3_4:-75721539 Msa0522520:exon
CTTGTCACTGTTCTCGATGT+TGG 0.470383 3_4:-75720385 Msa0522520:CDS
GTTGTTCTTATTATCTGGTA+TGG 0.471581 3_4:+75721478 None:intergenic
TTTGATATTGAGTTAACTCT+AGG 0.472211 3_4:+75721227 None:intergenic
TTGGAGGAGACAGCAAGAAC+AGG 0.474369 3_4:-75719348 Msa0522520:CDS
GGGCTTTCAATCTGATGATC+CGG 0.476077 3_4:-75719849 Msa0522520:CDS
CGTGAAAACGGGGCAGGGGA+AGG 0.476652 3_4:-75721028 Msa0522520:CDS
GGCTGAGAAGCTGGCTAGGA+TGG 0.477540 3_4:-75719216 Msa0522520:CDS
ATCCTTCCACCACCAGAAGT+TGG 0.478748 3_4:+75720712 None:intergenic
TGGCTCGGCGTATCGTTGAC+CGG 0.478939 3_4:-75720204 Msa0522520:CDS
ACTGACACTGTTACCTTCAC+CGG 0.479335 3_4:+75720174 None:intergenic
AGACCCACGCTGTTGAGCTT+AGG 0.482490 3_4:-75719785 Msa0522520:CDS
TTCAGGGTCTCGTGTACAAA+AGG 0.483863 3_4:+75721505 None:intergenic
GTTGAGAAGAATGACGGCTC+AGG 0.487316 3_4:-75720236 Msa0522520:CDS
AGCACTGATCTCGGCGGCCC+GGG 0.490522 3_4:+75719826 None:intergenic
ATCATCTTCTAATGCACAAA+GGG 0.491038 3_4:+75718975 None:intergenic
ATCCGACGGTCAAGATGAAA+AGG 0.492993 3_4:-75720077 Msa0522520:CDS
CTCAGGGTCAACGTTTGGCT+CGG 0.493080 3_4:-75720219 Msa0522520:CDS
AGGAGACTTTGTTGATCTTG+GGG 0.496143 3_4:+75721247 None:intergenic
TAGCACTGATCTCGGCGGCC+CGG 0.497684 3_4:+75719825 None:intergenic
TCAGCTGAGCAATATGTTCG+TGG 0.499359 3_4:-75719398 Msa0522520:CDS
GAATTTAGTTTAGGGAGCAT+GGG 0.504116 3_4:+75721690 None:intergenic
GTATACTATCTCTTGACTTG+CGG 0.504508 3_4:+75719634 None:intergenic
GACCGGCTTGTTACCGGTGA+AGG 0.505460 3_4:-75720187 Msa0522520:CDS
TGAATCACCGACTCCATCGC+TGG 0.506205 3_4:+75720561 None:intergenic
GAACAGCGTGAAAACGGGGC+AGG 0.506795 3_4:-75721034 Msa0522520:CDS
CTCCTTTCTCCAACTTCTGG+TGG 0.508187 3_4:-75720721 Msa0522520:CDS
GTCACTGTTCTCGATGTTGG+TGG 0.508779 3_4:-75720382 Msa0522520:CDS
CAAGCTGAAGTAGGTGTAAG+TGG 0.509486 3_4:+75719245 None:intergenic
ATGGACTATGATCTTGAAAA+GGG 0.509533 3_4:+75718831 None:intergenic
TGTATGGCCCACCTTTAAAT+GGG 0.510023 3_4:-75718940 Msa0522520:three_prime_UTR
TCAATCTGATGATCCGGCCC+GGG 0.510856 3_4:-75719843 Msa0522520:CDS
AAAACAATTGGGGATGGGAA+TGG 0.515689 3_4:-75719024 Msa0522520:three_prime_UTR
TCCATGTGGCGTTGCAGACA+GGG 0.516401 3_4:+75720923 None:intergenic
TATCTGGTATGGTAGATTCA+GGG 0.519491 3_4:+75721489 None:intergenic
AGCTGGCGGAGCTTCATTGG+AGG 0.520138 3_4:-75719364 Msa0522520:CDS
TTTGTGGTTTGGAATTGCAT+TGG 0.521882 3_4:+75720846 None:intergenic
AATAAAGTCAGTGACTTACA+TGG 0.522082 3_4:-75719173 Msa0522520:CDS
AAGTTCAACGAACTAGACTT+TGG 0.524651 3_4:+75721804 None:intergenic
AGCTTAGGAGCCGTACGGCT+CGG 0.525452 3_4:-75719770 Msa0522520:CDS
TCTCAAAGTCTGAAACAAAA+GGG 0.527985 3_4:-75721584 Msa0522520:five_prime_UTR
TGATTATGTGATGGAGTGTA+TGG 0.529524 3_4:-75718956 Msa0522520:three_prime_UTR
CCAGCACACTTGATCCTTCA+CGG 0.529684 3_4:-75720423 Msa0522520:CDS
TTCTTGTGCGCCGCTAGAAG+AGG 0.529764 3_4:+75720892 None:intergenic
ATTGTTCGACAACGAAGACT+TGG 0.533284 3_4:+75721332 None:intergenic
TTTGCAAATGCATCCTCACC+CGG 0.533505 3_4:+75719923 None:intergenic
TGAGCAATATGTTCGTGGCT+TGG 0.534296 3_4:-75719393 Msa0522520:CDS
TTGAAGAGGGACAACTTCAA+AGG 0.534296 3_4:+75721185 None:intergenic
ATCGCTGGTTTGAATGTCAT+CGG 0.535321 3_4:+75720576 None:intergenic
GAGCGGCTGAGAAGCTGGCT+AGG 0.536165 3_4:-75719220 Msa0522520:CDS
TGATGATGCAAAGAAGAAAA+GGG 0.537102 3_4:-75719298 Msa0522520:CDS
AACAGCGTGAAAACGGGGCA+GGG 0.537796 3_4:-75721033 Msa0522520:CDS
CCCACCATGTGATGACGGTA+AGG 0.540505 3_4:-75719673 Msa0522520:CDS
GTGCTGGTTCCGCCGCGGAG+TGG 0.541711 3_4:+75720439 None:intergenic
AGGAAGAAAACAATTGGGGA+TGG 0.542265 3_4:-75719030 Msa0522520:three_prime_UTR
AATTTCAATATGAGCGAACC+GGG 0.543870 3_4:+75719994 None:intergenic
CACCACCAGAAGTTGGAGAA+AGG 0.546899 3_4:+75720719 None:intergenic
TCTATCGGAGCTCGCCGTGA+AGG 0.548944 3_4:+75720409 None:intergenic
CACTTACACCTACTTCAGCT+TGG 0.550476 3_4:-75719244 Msa0522520:CDS
CAAATTCTCCCATTTAAAGG+TGG 0.550723 3_4:+75718932 None:intergenic
CCGTGAAGGATCAAGTGTGC+TGG 0.551020 3_4:+75720423 None:intergenic
GCCCTGTCTGCAACGCCACA+TGG 0.554667 3_4:-75720924 Msa0522520:CDS
GGACAAGGATCGAAAGGTTG+AGG 0.556824 3_4:-75719547 Msa0522520:CDS
AGAGTATATAGCACTGATCT+CGG 0.558479 3_4:+75719817 None:intergenic
TGTTTGATTCAGAGTAGCTG+TGG 0.558848 3_4:-75721066 Msa0522520:intron
TACATGGTTTCGAAAATGCT+AGG 0.561353 3_4:-75719157 Msa0522520:CDS
CGTACATGTGCTGCTATGCA+AGG 0.562258 3_4:+75720967 None:intergenic
AAATTCTCCCATTTAAAGGT+GGG 0.565551 3_4:+75718933 None:intergenic
ACAGCGTGAAAACGGGGCAG+GGG 0.566472 3_4:-75721032 Msa0522520:CDS
TTGGTTCTGATAACAGGAGA+GGG 0.567897 3_4:+75720814 None:intergenic
TTATAGTCATGAACCGGGTG+AGG 0.569511 3_4:-75719936 Msa0522520:CDS
GAAGCTGGCTAGGATGGCTA+TGG 0.570165 3_4:-75719210 Msa0522520:CDS
TGGCTTGGAAGCTGAGCTGG+CGG 0.571971 3_4:-75719378 Msa0522520:CDS
TAGCATGTGAAGCTTTGCGC+TGG 0.574375 3_4:+75720351 None:intergenic
GGAAGAAAACAATTGGGGAT+GGG 0.575040 3_4:-75719029 Msa0522520:three_prime_UTR
ATATTCTGAGATTGATTCTG+AGG 0.576073 3_4:+75719581 None:intergenic
CAGGGCAAACAAGGGTAGCA+TGG 0.577383 3_4:+75720941 None:intergenic
AAGCCTCCTTGACTCAGCTA+TGG 0.578141 3_4:+75719502 None:intergenic
TTGAGAAGAATGACGGCTCA+GGG 0.581626 3_4:-75720235 Msa0522520:CDS
GATGCATTTGCAAAATGTGT+TGG 0.582126 3_4:-75719914 Msa0522520:CDS
CGTGAATGAAAATGGGTACT+GGG 0.582287 3_4:-75721538 Msa0522520:exon
TACACAAAATACAAACAGCC+AGG 0.582719 3_4:-75721921 None:intergenic
GCATTTGCAAAATGTGTTGG+CGG 0.583323 3_4:-75719911 Msa0522520:CDS
GTATAAATCGCCGAGCCGTA+CGG 0.585496 3_4:+75719760 None:intergenic
CTGGTTCCGCCGCGGAGTGG+CGG 0.587009 3_4:+75720442 None:intergenic
ACAACCACGCTTCTTCAACC+CGG 0.593144 3_4:-75720012 Msa0522520:CDS
CGGGTCCCACCATGTGATGA+CGG 0.594441 3_4:-75719678 Msa0522520:CDS
TTTGGGTTTGGTAGCAAAAG+CGG 0.594571 3_4:-75719959 Msa0522520:CDS
GAAGTTGGAGAAAGGAGTAG+AGG 0.596574 3_4:+75720727 None:intergenic
AGAATCAAAATCAAAATCAA+CGG 0.596605 3_4:-75720042 Msa0522520:CDS
ATAGTATACGGCAGAGAACA+GGG 0.598674 3_4:-75719620 Msa0522520:CDS
AAAAGGGTGCGAAAATATGG+TGG 0.598978 3_4:-75719282 Msa0522520:CDS
TACCTTCACCGGTAACAAGC+CGG 0.599061 3_4:+75720185 None:intergenic
AATCATCTTCTAATGCACAA+AGG 0.599824 3_4:+75718974 None:intergenic
TGTTCTGGAAACTGATACGA+TGG 0.602317 3_4:+75720504 None:intergenic
GGAGACAAGGTGGTAGCAGA+CGG 0.603225 3_4:-75719003 Msa0522520:three_prime_UTR
GATTTAGATTGGTGCAAACG+TGG 0.604999 3_4:+75721371 None:intergenic
AGAACAGTGACAAGATCTAT+CGG 0.606607 3_4:+75720394 None:intergenic
TAGAGGCTCGTCATCATCGT+AGG 0.609610 3_4:+75720744 None:intergenic
CAACTGGGACAAGGATCGAA+AGG 0.612419 3_4:-75719553 Msa0522520:CDS
GCCGCTCTCCAAGCTGAAGT+AGG 0.612752 3_4:+75719236 None:intergenic
TTAGAGGAAGAAAACAATTG+GGG 0.614763 3_4:-75719034 Msa0522520:three_prime_UTR
TGGGGATGGGAATGGAGACA+AGG 0.616259 3_4:-75719016 Msa0522520:three_prime_UTR
TCTCGAGGCGTTTAACTGCA+GGG 0.616353 3_4:-75721858 Msa0522520:five_prime_UTR
ACCTACTTCAGCTTGGAGAG+CGG 0.616379 3_4:-75719237 Msa0522520:CDS
GCAAGAACAGGTTCAAGTTG+AGG 0.620780 3_4:-75719336 Msa0522520:CDS
GATGACATTCAAACCAGCGA+TGG 0.621098 3_4:-75720574 Msa0522520:CDS
GACAACTTCAAAGGAGAGCG+AGG 0.621814 3_4:+75721194 None:intergenic
CTTGGTTCTGATAACAGGAG+AGG 0.622597 3_4:+75720813 None:intergenic
GGATGGGAATGGAGACAAGG+TGG 0.622694 3_4:-75719013 Msa0522520:three_prime_UTR
CACGAGCCATAGCTGAGTCA+AGG 0.623356 3_4:-75719508 Msa0522520:CDS
CTCAGAATATACGATCAACT+GGG 0.623485 3_4:-75719568 Msa0522520:CDS
TATCAAAAGAGCACGAGCCG+AGG 0.628477 3_4:+75719436 None:intergenic
CAGGATTTAAGAGTTCAACT+GGG 0.628767 3_4:-75719869 Msa0522520:CDS
GGCTCCTAAGCTCAACAGCG+TGG 0.629591 3_4:+75719781 None:intergenic
AGCGTCATGCTGTTATCCGA+CGG 0.629723 3_4:-75720091 Msa0522520:CDS
ACAGCATGACGCTAGCAACC+GGG 0.631559 3_4:+75720101 None:intergenic
GCTCCTAAGCTCAACAGCGT+GGG 0.636528 3_4:+75719782 None:intergenic
GACACCTTACCGTCATCACA+TGG 0.636879 3_4:+75719669 None:intergenic
ACAATTGAAAGCCTATCAGA+AGG 0.637171 3_4:+75720292 None:intergenic
CCTTACCGTCATCACATGGT+GGG 0.641950 3_4:+75719673 None:intergenic
ATTCAAACCAGCGATGGAGT+CGG 0.646816 3_4:-75720568 Msa0522520:CDS
AGTGCTATATACTCTTGCAG+TGG 0.648460 3_4:-75719809 Msa0522520:CDS
CCGTACCCTAAAGCTGACGC+TGG 0.648963 3_4:+75719698 None:intergenic
AAAGGTGACGCAGAAGACAA+AGG 0.649534 3_4:+75721668 None:intergenic
CGAAGCAGATGAAAATGCAG+AGG 0.652962 3_4:-75720677 Msa0522520:CDS
CTTACCGTTGAGAAGAATGA+CGG 0.653691 3_4:-75720242 Msa0522520:CDS
AATAAGAACAACAACAACAA+CGG 0.659208 3_4:-75721467 Msa0522520:CDS
GCACCACCGCCACTCCGCGG+CGG 0.660211 3_4:-75720448 Msa0522520:CDS
ATATACGATCAACTGGGACA+AGG 0.662088 3_4:-75719562 Msa0522520:CDS
AGCGGTTATAGTCATGAACC+GGG 0.663417 3_4:-75719941 Msa0522520:CDS
GTTTGAACAGCGTGAAAACG+GGG 0.664529 3_4:-75721038 Msa0522520:CDS
TGAGCCGTCATTCTTCTCAA+CGG 0.667424 3_4:+75720238 None:intergenic
ATACTATCTCTTGACTTGCG+GGG 0.673555 3_4:+75719636 None:intergenic
ACCTTACCGTCATCACATGG+TGG 0.676357 3_4:+75719672 None:intergenic
TGTTGAGCTTAGGAGCCGTA+CGG 0.693392 3_4:-75719775 Msa0522520:CDS
AAAGCACCACCGCCACTCCG+CGG 0.698878 3_4:-75720451 Msa0522520:CDS
ATTAGAAGATGATTATGTGA+TGG 0.699218 3_4:-75718965 Msa0522520:three_prime_UTR
TGTTATCAGAACCAAGAACG+TGG 0.700341 3_4:-75720806 Msa0522520:CDS
CAAGTGTGCTGGTTCCGCCG+CGG 0.700524 3_4:+75720434 None:intergenic
GGGAGAATAGATCTCTCTCG+AGG 0.702517 3_4:-75721873 Msa0522520:five_prime_UTR
GAGCCATAGCTGAGTCAAGG+AGG 0.706855 3_4:-75719505 Msa0522520:CDS
GTTGCAGACAGGGCAAACAA+GGG 0.707906 3_4:+75720933 None:intergenic
AAGAGGAACGTCTTTCCATG+TGG 0.708069 3_4:+75720909 None:intergenic
GTACATGTGCTGCTATGCAA+GGG 0.711015 3_4:+75720968 None:intergenic
CGAACCGGGTTGAAGAAGCG+TGG 0.713159 3_4:+75720008 None:intergenic
GAAAGACGTTCCTCTTCTAG+CGG 0.718880 3_4:-75720902 Msa0522520:CDS
GGATCGTAGAGAAAGAAACC+CGG 0.722882 3_4:-75720119 Msa0522520:CDS
GTATATAGCACTGATCTCGG+CGG 0.740345 3_4:+75719820 None:intergenic
GAATGAAAATGGGTACTGGG+TGG 0.746218 3_4:-75721535 Msa0522520:CDS
CAAGGTGGTAGCAGACGGCG+CGG 0.753167 3_4:-75718998 Msa0522520:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATAAAATAGAATAGAA+TGG + chr3_4:75718968-75718987 None:intergenic 10.0%
!!! TTATTTTTATTATCTTGATA+TGG - chr3_4:75718992-75719011 Msa0522520:three_prime_UTR 10.0%
!! AATAACATATATAGTAAAAA+AGG + chr3_4:75719627-75719646 None:intergenic 10.0%
!! ATAATATCAGTATCATTATT+TGG + chr3_4:75719655-75719674 None:intergenic 15.0%
!! AAAGAACTTATTATAATTAG+AGG - chr3_4:75721691-75721710 Msa0522520:five_prime_UTR 15.0%
!! AGAATCAAAATCAAAATCAA+CGG - chr3_4:75720699-75720718 Msa0522520:CDS 20.0%
!! AGACAAAGGAATTTAGTTTA+GGG + chr3_4:75719062-75719081 None:intergenic 25.0%
!! AAGACAAAGGAATTTAGTTT+AGG + chr3_4:75719063-75719082 None:intergenic 25.0%
!!! TGTTTGTTTTGAAGAAAGAA+TGG + chr3_4:75719104-75719123 None:intergenic 25.0%
!! TTGTTGTTGTTCTTATTATC+TGG + chr3_4:75719271-75719290 None:intergenic 25.0%
! AATAAGAACAACAACAACAA+CGG - chr3_4:75719274-75719293 Msa0522520:CDS 25.0%
! TTTGATATTGAGTTAACTCT+AGG + chr3_4:75719517-75719536 None:intergenic 25.0%
! AATTAGAGGAAGAAAACAAT+TGG - chr3_4:75721705-75721724 Msa0522520:five_prime_UTR 25.0%
! ATTAGAGGAAGAAAACAATT+GGG - chr3_4:75721706-75721725 Msa0522520:five_prime_UTR 25.0%
! ATTAGAAGATGATTATGTGA+TGG - chr3_4:75721776-75721795 Msa0522520:five_prime_UTR 25.0%
! AATCAAATTCTCCCATTTAA+AGG + chr3_4:75721815-75721834 None:intergenic 25.0%
! TTTAAATGGGAGAATTTGAT+TGG - chr3_4:75721814-75721833 Msa0522520:five_prime_UTR 25.0%
GAAGAAAGAATGGATCTAAA+AGG + chr3_4:75719094-75719113 None:intergenic 30.0%
! TTCTCAAAGTCTGAAACAAA+AGG - chr3_4:75719156-75719175 Msa0522520:CDS 30.0%
! TCTCAAAGTCTGAAACAAAA+GGG - chr3_4:75719157-75719176 Msa0522520:CDS 30.0%
! TACCTTTTCGTGAATGAAAA+TGG - chr3_4:75719195-75719214 Msa0522520:CDS 30.0%
! ACCTTTTCGTGAATGAAAAT+GGG - chr3_4:75719196-75719215 Msa0522520:CDS 30.0%
GTTGTTCTTATTATCTGGTA+TGG + chr3_4:75719266-75719285 None:intergenic 30.0%
AAACTGGTTGAGATTTAGAT+TGG + chr3_4:75719384-75719403 None:intergenic 30.0%
!!! TTTTGCTACTTTGAAATCTG+GGG + chr3_4:75719468-75719487 None:intergenic 30.0%
!!! CTTTTGCTACTTTGAAATCT+GGG + chr3_4:75719469-75719488 None:intergenic 30.0%
!!! ACTTTTGCTACTTTGAAATC+TGG + chr3_4:75719470-75719489 None:intergenic 30.0%
!!! TTTGAAGCTGTTTTTGAAGA+GGG + chr3_4:75719572-75719591 None:intergenic 30.0%
!!! ATTTGAAGCTGTTTTTGAAG+AGG + chr3_4:75719573-75719592 None:intergenic 30.0%
TCAAAAACAGCTTCAAATTC+AGG - chr3_4:75719575-75719594 Msa0522520:CDS 30.0%
CAAAAACAGCTTCAAATTCA+GGG - chr3_4:75719576-75719595 Msa0522520:CDS 30.0%
!! ATATTCTGAGATTGATTCTG+AGG + chr3_4:75721163-75721182 None:intergenic 30.0%
! ATGATGATGCAAAGAAGAAA+AGG - chr3_4:75721442-75721461 Msa0522520:CDS 30.0%
! TGATGATGCAAAGAAGAAAA+GGG - chr3_4:75721443-75721462 Msa0522520:CDS 30.0%
!! GAAAATATGGTGGATGAAAA+TGG - chr3_4:75721469-75721488 Msa0522520:CDS 30.0%
AATAAAGTCAGTGACTTACA+TGG - chr3_4:75721568-75721587 Msa0522520:five_prime_UTR 30.0%
TTAGAGGAAGAAAACAATTG+GGG - chr3_4:75721707-75721726 Msa0522520:five_prime_UTR 30.0%
ATCATCTTCTAATGCACAAA+GGG + chr3_4:75721769-75721788 None:intergenic 30.0%
AATCATCTTCTAATGCACAA+AGG + chr3_4:75721770-75721789 None:intergenic 30.0%
AAATTCTCCCATTTAAAGGT+GGG + chr3_4:75721811-75721830 None:intergenic 30.0%
GAGAAGACAAAAAGATATGA+CGG + chr3_4:75721849-75721868 None:intergenic 30.0%
AAGTTCAACGAACTAGACTT+TGG + chr3_4:75718940-75718959 None:intergenic 35.0%
! GAATTTAGTTTAGGGAGCAT+GGG + chr3_4:75719054-75719073 None:intergenic 35.0%
! ACCCATTTTCATTCACGAAA+AGG + chr3_4:75719200-75719219 None:intergenic 35.0%
TATCTGGTATGGTAGATTCA+GGG + chr3_4:75719255-75719274 None:intergenic 35.0%
TTATCTGGTATGGTAGATTC+AGG + chr3_4:75719256-75719275 None:intergenic 35.0%
! GCTAGATTGAGTTTTCTTTC+TGG - chr3_4:75719325-75719344 Msa0522520:CDS 35.0%
! AGATTGAGTTTTCTTTCTGG+TGG - chr3_4:75719328-75719347 Msa0522520:CDS 35.0%
TAGGAGACTTTGTTGATCTT+GGG + chr3_4:75719498-75719517 None:intergenic 35.0%
! TTGTGGTTTGGAATTGCATT+GGG + chr3_4:75719897-75719916 None:intergenic 35.0%
! TTTGTGGTTTGGAATTGCAT+TGG + chr3_4:75719898-75719917 None:intergenic 35.0%
! GGATTAGGTATTTTGTGGTT+TGG + chr3_4:75719909-75719928 None:intergenic 35.0%
AGAACAGTGACAAGATCTAT+CGG + chr3_4:75720350-75720369 None:intergenic 35.0%
! CGTTTGGTTATTTCATCTCT+TGG - chr3_4:75720425-75720444 Msa0522520:CDS 35.0%
ACAATTGAAAGCCTATCAGA+AGG + chr3_4:75720452-75720471 None:intergenic 35.0%
!! CTTTTTCGTCTGTTTCCAAA+CGG - chr3_4:75720474-75720493 Msa0522520:CDS 35.0%
! GAGAAAAGCAACTACAGTTT+TGG - chr3_4:75720598-75720617 Msa0522520:CDS 35.0%
!! AAAAGCAACTACAGTTTTGG+AGG - chr3_4:75720601-75720620 Msa0522520:CDS 35.0%
AATTTCAATATGAGCGAACC+GGG + chr3_4:75720750-75720769 None:intergenic 35.0%
GAATTTCAATATGAGCGAAC+CGG + chr3_4:75720751-75720770 None:intergenic 35.0%
GTTCGCTCATATTGAAATTC+CGG - chr3_4:75720751-75720770 Msa0522520:CDS 35.0%
GATGCATTTGCAAAATGTGT+TGG - chr3_4:75720827-75720846 Msa0522520:CDS 35.0%
TCAGGATTTAAGAGTTCAAC+TGG - chr3_4:75720871-75720890 Msa0522520:CDS 35.0%
CAGGATTTAAGAGTTCAACT+GGG - chr3_4:75720872-75720891 Msa0522520:CDS 35.0%
!! AGAGTATATAGCACTGATCT+CGG + chr3_4:75720927-75720946 None:intergenic 35.0%
! TGAAGAAGAAAGAGAGCTTT+TGG - chr3_4:75721006-75721025 Msa0522520:CDS 35.0%
!! TATACTATCTCTTGACTTGC+GGG + chr3_4:75721109-75721128 None:intergenic 35.0%
! GTATACTATCTCTTGACTTG+CGG + chr3_4:75721110-75721129 None:intergenic 35.0%
GCAAGTCAAGAGATAGTATA+CGG - chr3_4:75721109-75721128 Msa0522520:intron 35.0%
TCTCAGAATATACGATCAAC+TGG - chr3_4:75721172-75721191 Msa0522520:CDS 35.0%
CTCAGAATATACGATCAACT+GGG - chr3_4:75721173-75721192 Msa0522520:CDS 35.0%
! AAGAAAAGGGTGCGAAAATA+TGG - chr3_4:75721456-75721475 Msa0522520:CDS 35.0%
TACATGGTTTCGAAAATGCT+AGG - chr3_4:75721584-75721603 Msa0522520:five_prime_UTR 35.0%
! TGATTATGTGATGGAGTGTA+TGG - chr3_4:75721785-75721804 Msa0522520:five_prime_UTR 35.0%
CAAATTCTCCCATTTAAAGG+TGG + chr3_4:75721812-75721831 None:intergenic 35.0%
AATGGGAGAATTTGATTGGT+TGG - chr3_4:75721818-75721837 Msa0522520:five_prime_UTR 35.0%
! GGAATTTAGTTTAGGGAGCA+TGG + chr3_4:75719055-75719074 None:intergenic 40.0%
TCGTGAATGAAAATGGGTAC+TGG - chr3_4:75719202-75719221 Msa0522520:CDS 40.0%
CGTGAATGAAAATGGGTACT+GGG - chr3_4:75719203-75719222 Msa0522520:CDS 40.0%
GATTTAGATTGGTGCAAACG+TGG + chr3_4:75719373-75719392 None:intergenic 40.0%
ATTGTTCGACAACGAAGACT+TGG + chr3_4:75719412-75719431 None:intergenic 40.0%
AGGAGACTTTGTTGATCTTG+GGG + chr3_4:75719497-75719516 None:intergenic 40.0%
CTAGGAGACTTTGTTGATCT+TGG + chr3_4:75719499-75719518 None:intergenic 40.0%
TTGAAGAGGGACAACTTCAA+AGG + chr3_4:75719559-75719578 None:intergenic 40.0%
TGTTTGATTCAGAGTAGCTG+TGG - chr3_4:75719675-75719694 Msa0522520:CDS 40.0%
CTGTTTGAACAGCGTGAAAA+CGG - chr3_4:75719701-75719720 Msa0522520:CDS 40.0%
TGTTTGAACAGCGTGAAAAC+GGG - chr3_4:75719702-75719721 Msa0522520:CDS 40.0%
! GAGAGGGATTAGGTATTTTG+TGG + chr3_4:75719914-75719933 None:intergenic 40.0%
!! TTGGTTCTGATAACAGGAGA+GGG + chr3_4:75719930-75719949 None:intergenic 40.0%
!! ACGTTCTTGGTTCTGATAAC+AGG + chr3_4:75719936-75719955 None:intergenic 40.0%
TGTTATCAGAACCAAGAACG+TGG - chr3_4:75719935-75719954 Msa0522520:CDS 40.0%
! TCTGCATTTTCATCTGCTTC+GGG + chr3_4:75720065-75720084 None:intergenic 40.0%
! CTCTGCATTTTCATCTGCTT+CGG + chr3_4:75720066-75720085 None:intergenic 40.0%
!! ATCGGAGTACGTTTTGTTAG+CGG + chr3_4:75720150-75720169 None:intergenic 40.0%
!! ATCGCTGGTTTGAATGTCAT+CGG + chr3_4:75720168-75720187 None:intergenic 40.0%
!! TGTTCTGGAAACTGATACGA+TGG + chr3_4:75720240-75720259 None:intergenic 40.0%
AACACACGAAACATACGCTT+TGG - chr3_4:75720256-75720275 Msa0522520:CDS 40.0%
CTTACCGTTGAGAAGAATGA+CGG - chr3_4:75720499-75720518 Msa0522520:CDS 40.0%
! AACCTTTTCATCTTGACCGT+CGG + chr3_4:75720669-75720688 None:intergenic 40.0%
AAATTCCGGTTCATGCGTTT+GGG - chr3_4:75720765-75720784 Msa0522520:CDS 40.0%
! TTTGGGTTTGGTAGCAAAAG+CGG - chr3_4:75720782-75720801 Msa0522520:CDS 40.0%
AAGCGGTTATAGTCATGAAC+CGG - chr3_4:75720799-75720818 Msa0522520:CDS 40.0%
GCATTTGCAAAATGTGTTGG+CGG - chr3_4:75720830-75720849 Msa0522520:CDS 40.0%
!! TCTTTTGAGTGTTGTGGTTC+AGG - chr3_4:75720853-75720872 Msa0522520:CDS 40.0%
AGTGCTATATACTCTTGCAG+TGG - chr3_4:75720932-75720951 Msa0522520:CDS 40.0%
! AGAAGAAAGAGAGCTTTTGG+TGG - chr3_4:75721009-75721028 Msa0522520:CDS 40.0%
!! ATACTATCTCTTGACTTGCG+GGG + chr3_4:75721108-75721127 None:intergenic 40.0%
ATAGTATACGGCAGAGAACA+GGG - chr3_4:75721121-75721140 Msa0522520:intron 40.0%
ATATACGATCAACTGGGACA+AGG - chr3_4:75721179-75721198 Msa0522520:CDS 40.0%
TGCTCATCATTTACTTGCCT+CGG - chr3_4:75721288-75721307 Msa0522520:CDS 40.0%
! CGTGCTCTTTTGATACAATC+TGG - chr3_4:75721313-75721332 Msa0522520:CDS 40.0%
! AAAAGGGTGCGAAAATATGG+TGG - chr3_4:75721459-75721478 Msa0522520:CDS 40.0%
AGGAAGAAAACAATTGGGGA+TGG - chr3_4:75721711-75721730 Msa0522520:five_prime_UTR 40.0%
GGAAGAAAACAATTGGGGAT+GGG - chr3_4:75721712-75721731 Msa0522520:five_prime_UTR 40.0%
AAAACAATTGGGGATGGGAA+TGG - chr3_4:75721717-75721736 Msa0522520:five_prime_UTR 40.0%
TGTATGGCCCACCTTTAAAT+GGG - chr3_4:75721801-75721820 Msa0522520:five_prime_UTR 40.0%
AAAGGTGACGCAGAAGACAA+AGG + chr3_4:75719076-75719095 None:intergenic 45.0%
!! GAATGAAAATGGGTACTGGG+TGG - chr3_4:75719206-75719225 Msa0522520:CDS 45.0%
TTCAGGGTCTCGTGTACAAA+AGG + chr3_4:75719239-75719258 None:intergenic 45.0%
! CAGCTTCTTGGACTAGGATT+AGG + chr3_4:75719304-75719323 None:intergenic 45.0%
CTCAATCTAGCACAGCTTCT+TGG + chr3_4:75719316-75719335 None:intergenic 45.0%
! CGAAGACTTGGACTAGAAAC+TGG + chr3_4:75719400-75719419 None:intergenic 45.0%
GTTTGAACAGCGTGAAAACG+GGG - chr3_4:75719703-75719722 Msa0522520:CDS 45.0%
! GTACATGTGCTGCTATGCAA+GGG + chr3_4:75719776-75719795 None:intergenic 45.0%
AAGAGGAACGTCTTTCCATG+TGG + chr3_4:75719835-75719854 None:intergenic 45.0%
GAAAGACGTTCCTCTTCTAG+CGG - chr3_4:75719839-75719858 Msa0522520:CDS 45.0%
CTGATAACAGGAGAGGGATT+AGG + chr3_4:75719924-75719943 None:intergenic 45.0%
!! CTTGGTTCTGATAACAGGAG+AGG + chr3_4:75719931-75719950 None:intergenic 45.0%
TTGAGACTGATCCACGTTCT+TGG + chr3_4:75719949-75719968 None:intergenic 45.0%
GAAGTTGGAGAAAGGAGTAG+AGG + chr3_4:75720017-75720036 None:intergenic 45.0%
CTACTCCTTTCTCCAACTTC+TGG - chr3_4:75720017-75720036 Msa0522520:CDS 45.0%
! CTGCATTTTCATCTGCTTCG+GGG + chr3_4:75720064-75720083 None:intergenic 45.0%
CGAAGCAGATGAAAATGCAG+AGG - chr3_4:75720064-75720083 Msa0522520:CDS 45.0%
GATGACAACTGCGAGTTTCA+AGG - chr3_4:75720095-75720114 Msa0522520:CDS 45.0%
!! GTTTTGTTAGCGGTGGAAGT+AGG + chr3_4:75720140-75720159 None:intergenic 45.0%
GATGACATTCAAACCAGCGA+TGG - chr3_4:75720167-75720186 Msa0522520:CDS 45.0%
ATTCAAACCAGCGATGGAGT+CGG - chr3_4:75720173-75720192 Msa0522520:CDS 45.0%
CGGTGATTCAAGAACTGTTC+AGG - chr3_4:75720193-75720212 Msa0522520:CDS 45.0%
ACTGATACGATGGCACATTC+TGG + chr3_4:75720230-75720249 None:intergenic 45.0%
AGCGTATGTTTCGTGTGTTC+TGG + chr3_4:75720255-75720274 None:intergenic 45.0%
CTTGTCACTGTTCTCGATGT+TGG - chr3_4:75720356-75720375 Msa0522520:CDS 45.0%
CATCTCTTGGTCCTTCTGAT+AGG - chr3_4:75720438-75720457 Msa0522520:CDS 45.0%
TGAGCCGTCATTCTTCTCAA+CGG + chr3_4:75720506-75720525 None:intergenic 45.0%
TTGAGAAGAATGACGGCTCA+GGG - chr3_4:75720506-75720525 Msa0522520:CDS 45.0%
ACTGACACTGTTACCTTCAC+CGG + chr3_4:75720570-75720589 None:intergenic 45.0%
GGATCGTAGAGAAAGAAACC+CGG - chr3_4:75720622-75720641 Msa0522520:CDS 45.0%
ATCCGACGGTCAAGATGAAA+AGG - chr3_4:75720664-75720683 Msa0522520:CDS 45.0%
GAAATTCCGGTTCATGCGTT+TGG - chr3_4:75720764-75720783 Msa0522520:CDS 45.0%
AGCGGTTATAGTCATGAACC+GGG - chr3_4:75720800-75720819 Msa0522520:CDS 45.0%
TTATAGTCATGAACCGGGTG+AGG - chr3_4:75720805-75720824 Msa0522520:CDS 45.0%
TTTGCAAATGCATCCTCACC+CGG + chr3_4:75720821-75720840 None:intergenic 45.0%
GGGCTTTCAATCTGATGATC+CGG - chr3_4:75720892-75720911 Msa0522520:CDS 45.0%
!! GTATATAGCACTGATCTCGG+CGG + chr3_4:75720924-75720943 None:intergenic 45.0%
GATAGTATACGGCAGAGAAC+AGG - chr3_4:75721120-75721139 Msa0522520:intron 45.0%
TCAGCTGAGCAATATGTTCG+TGG - chr3_4:75721343-75721362 Msa0522520:CDS 45.0%
TGAGCAATATGTTCGTGGCT+TGG - chr3_4:75721348-75721367 Msa0522520:CDS 45.0%
!! GCAAGAACAGGTTCAAGTTG+AGG - chr3_4:75721405-75721424 Msa0522520:CDS 45.0%
CAAGCTGAAGTAGGTGTAAG+TGG + chr3_4:75721499-75721518 None:intergenic 45.0%
CACTTACACCTACTTCAGCT+TGG - chr3_4:75721497-75721516 Msa0522520:CDS 45.0%
GTGTATGGCCCACCTTTAAA+TGG - chr3_4:75721800-75721819 Msa0522520:five_prime_UTR 45.0%
!!! ATGTTTTTTTTTTTTTTTAT+TGG - chr3_4:75721617-75721636 Msa0522520:five_prime_UTR 5.0%
!!! TGTTTTTTTTTTTTTTTATT+GGG - chr3_4:75721618-75721637 Msa0522520:five_prime_UTR 5.0%
TCTCTCTCTCTCTCTCTACC+TGG + chr3_4:75718841-75718860 None:intergenic 50.0%
GGGAGAATAGATCTCTCTCG+AGG - chr3_4:75718868-75718887 Msa0522520:three_prime_UTR 50.0%
! TCTCGAGGCGTTTAACTGCA+GGG - chr3_4:75718883-75718902 Msa0522520:three_prime_UTR 50.0%
CTAGCACAGCTTCTTGGACT+AGG + chr3_4:75719310-75719329 None:intergenic 50.0%
TGGTGCAAACGTGGAGTTGA+AGG + chr3_4:75719364-75719383 None:intergenic 50.0%
AAAGGAGAGCGAGGAGAAGT+TGG + chr3_4:75719541-75719560 None:intergenic 50.0%
GACAACTTCAAAGGAGAGCG+AGG + chr3_4:75719550-75719569 None:intergenic 50.0%
! CGTACATGTGCTGCTATGCA+AGG + chr3_4:75719777-75719796 None:intergenic 50.0%
GTTGCAGACAGGGCAAACAA+GGG + chr3_4:75719811-75719830 None:intergenic 50.0%
TAGAGGCTCGTCATCATCGT+AGG + chr3_4:75720000-75720019 None:intergenic 50.0%
CTCCTTTCTCCAACTTCTGG+TGG - chr3_4:75720020-75720039 Msa0522520:CDS 50.0%
CACCACCAGAAGTTGGAGAA+AGG + chr3_4:75720025-75720044 None:intergenic 50.0%
CTTTCTCCAACTTCTGGTGG+TGG - chr3_4:75720023-75720042 Msa0522520:CDS 50.0%
ATCCTTCCACCACCAGAAGT+TGG + chr3_4:75720032-75720051 None:intergenic 50.0%
!! GGAGTACGTTTTGTTAGCGG+TGG + chr3_4:75720147-75720166 None:intergenic 50.0%
CCAGCACACTTGATCCTTCA+CGG - chr3_4:75720318-75720337 Msa0522520:CDS 50.0%
GTCACTGTTCTCGATGTTGG+TGG - chr3_4:75720359-75720378 Msa0522520:CDS 50.0%
TAGCATGTGAAGCTTTGCGC+TGG + chr3_4:75720393-75720412 None:intergenic 50.0%
TCTCAACGGTAAGAGCCGTT+TGG + chr3_4:75720492-75720511 None:intergenic 50.0%
GTTGAGAAGAATGACGGCTC+AGG - chr3_4:75720505-75720524 Msa0522520:CDS 50.0%
!! ATCGTTGACCGGCTTGTTAC+CGG - chr3_4:75720548-75720567 Msa0522520:CDS 50.0%
TACCTTCACCGGTAACAAGC+CGG + chr3_4:75720559-75720578 None:intergenic 50.0%
AACAGCATGACGCTAGCAAC+CGG + chr3_4:75720644-75720663 None:intergenic 50.0%
AGCGTCATGCTGTTATCCGA+CGG - chr3_4:75720650-75720669 Msa0522520:CDS 50.0%
ACAACCACGCTTCTTCAACC+CGG - chr3_4:75720729-75720748 Msa0522520:CDS 50.0%
CCAAACCCAAACGCATGAAC+CGG + chr3_4:75720773-75720792 None:intergenic 50.0%
!! TGGCGGTCTTTTGAGTGTTG+TGG - chr3_4:75720847-75720866 Msa0522520:CDS 50.0%
TTCAATCTGATGATCCGGCC+CGG - chr3_4:75720897-75720916 Msa0522520:CDS 50.0%
TGTTGAGCTTAGGAGCCGTA+CGG - chr3_4:75720966-75720985 Msa0522520:CDS 50.0%
GTATAAATCGCCGAGCCGTA+CGG + chr3_4:75720984-75721003 None:intergenic 50.0%
AGAGTTCCAGCGTCAGCTTT+AGG - chr3_4:75721037-75721056 Msa0522520:CDS 50.0%
GAGTTCCAGCGTCAGCTTTA+GGG - chr3_4:75721038-75721057 Msa0522520:CDS 50.0%
CCTTACCGTCATCACATGGT+GGG + chr3_4:75721071-75721090 None:intergenic 50.0%
ACCTTACCGTCATCACATGG+TGG + chr3_4:75721072-75721091 None:intergenic 50.0%
GACACCTTACCGTCATCACA+TGG + chr3_4:75721075-75721094 None:intergenic 50.0%
CAACTGGGACAAGGATCGAA+AGG - chr3_4:75721188-75721207 Msa0522520:CDS 50.0%
GGACAAGGATCGAAAGGTTG+AGG - chr3_4:75721194-75721213 Msa0522520:CDS 50.0%
AAGCCTCCTTGACTCAGCTA+TGG + chr3_4:75721242-75721261 None:intergenic 50.0%
! TATCAAAAGAGCACGAGCCG+AGG + chr3_4:75721308-75721327 None:intergenic 50.0%
TTGGAGGAGACAGCAAGAAC+AGG - chr3_4:75721393-75721412 Msa0522520:CDS 50.0%
ACCTACTTCAGCTTGGAGAG+CGG - chr3_4:75721504-75721523 Msa0522520:CDS 50.0%
AGAGAGAGAGAGAGAGAGCG+AGG - chr3_4:75718846-75718865 Msa0522520:three_prime_UTR 55.0%
GAGAGAGAGAGAGAGAGCGA+GGG - chr3_4:75718847-75718866 Msa0522520:three_prime_UTR 55.0%
AGAGAGAGAGAGAGAGCGAG+GGG - chr3_4:75718848-75718867 Msa0522520:three_prime_UTR 55.0%
! CTCTCGAGGCGTTTAACTGC+AGG - chr3_4:75718882-75718901 Msa0522520:three_prime_UTR 55.0%
AACAGCGTGAAAACGGGGCA+GGG - chr3_4:75719708-75719727 Msa0522520:CDS 55.0%
CAGGGCAAACAAGGGTAGCA+TGG + chr3_4:75719803-75719822 None:intergenic 55.0%
CGTTGCAGACAGGGCAAACA+AGG + chr3_4:75719812-75719831 None:intergenic 55.0%
TCCATGTGGCGTTGCAGACA+GGG + chr3_4:75719821-75719840 None:intergenic 55.0%
TTCCATGTGGCGTTGCAGAC+AGG + chr3_4:75719822-75719841 None:intergenic 55.0%
TTCTTGTGCGCCGCTAGAAG+AGG + chr3_4:75719852-75719871 None:intergenic 55.0%
!! CTCCAACTTCTGGTGGTGGA+AGG - chr3_4:75720027-75720046 Msa0522520:CDS 55.0%
!! TGAATCACCGACTCCATCGC+TGG + chr3_4:75720183-75720202 None:intergenic 55.0%
CCGTGAAGGATCAAGTGTGC+TGG + chr3_4:75720321-75720340 None:intergenic 55.0%
GCTAAAACGCGCGATGCGTT+TGG - chr3_4:75720409-75720428 Msa0522520:CDS 55.0%
CTCAGGGTCAACGTTTGGCT+CGG - chr3_4:75720522-75720541 Msa0522520:CDS 55.0%
ACAGCATGACGCTAGCAACC+GGG + chr3_4:75720643-75720662 None:intergenic 55.0%
! CCGGTTCATGCGTTTGGGTT+TGG - chr3_4:75720770-75720789 Msa0522520:CDS 55.0%
TCAATCTGATGATCCGGCCC+GGG - chr3_4:75720898-75720917 Msa0522520:CDS 55.0%
AGACCCACGCTGTTGAGCTT+AGG - chr3_4:75720956-75720975 Msa0522520:CDS 55.0%
GCTCCTAAGCTCAACAGCGT+GGG + chr3_4:75720962-75720981 None:intergenic 55.0%
!! CCAGCGTCAGCTTTAGGGTA+CGG - chr3_4:75721043-75721062 Msa0522520:CDS 55.0%
!! CAGCGTCAGCTTTAGGGTAC+GGG - chr3_4:75721044-75721063 Msa0522520:CDS 55.0%
!! CCCACCATGTGATGACGGTA+AGG - chr3_4:75721068-75721087 Msa0522520:intron 55.0%
CACGAGCCATAGCTGAGTCA+AGG - chr3_4:75721233-75721252 Msa0522520:CDS 55.0%
GAGCCATAGCTGAGTCAAGG+AGG - chr3_4:75721236-75721255 Msa0522520:CDS 55.0%
GAAGCTGGCTAGGATGGCTA+TGG - chr3_4:75721531-75721550 Msa0522520:CDS 55.0%
TGGGGATGGGAATGGAGACA+AGG - chr3_4:75721725-75721744 Msa0522520:five_prime_UTR 55.0%
GGATGGGAATGGAGACAAGG+TGG - chr3_4:75721728-75721747 Msa0522520:five_prime_UTR 55.0%
GGAGACAAGGTGGTAGCAGA+CGG - chr3_4:75721738-75721757 Msa0522520:five_prime_UTR 55.0%
GAACAGCGTGAAAACGGGGC+AGG - chr3_4:75719707-75719726 Msa0522520:CDS 60.0%
ACAGCGTGAAAACGGGGCAG+GGG - chr3_4:75719709-75719728 Msa0522520:CDS 60.0%
TCTATCGGAGCTCGCCGTGA+AGG + chr3_4:75720335-75720354 None:intergenic 60.0%
GACGGCTCAGGGTCAACGTT+TGG - chr3_4:75720517-75720536 Msa0522520:CDS 60.0%
! TGGCTCGGCGTATCGTTGAC+CGG - chr3_4:75720537-75720556 Msa0522520:CDS 60.0%
!! GACCGGCTTGTTACCGGTGA+AGG - chr3_4:75720554-75720573 Msa0522520:CDS 60.0%
! CGAACCGGGTTGAAGAAGCG+TGG + chr3_4:75720736-75720755 None:intergenic 60.0%
GGCTCCTAAGCTCAACAGCG+TGG + chr3_4:75720963-75720982 None:intergenic 60.0%
AGCTTAGGAGCCGTACGGCT+CGG - chr3_4:75720971-75720990 Msa0522520:CDS 60.0%
CCGTACCCTAAAGCTGACGC+TGG + chr3_4:75721046-75721065 None:intergenic 60.0%
CGGGTCCCACCATGTGATGA+CGG - chr3_4:75721063-75721082 Msa0522520:intron 60.0%
! TCGTGGCTTGGAAGCTGAGC+TGG - chr3_4:75721360-75721379 Msa0522520:CDS 60.0%
! TGGCTTGGAAGCTGAGCTGG+CGG - chr3_4:75721363-75721382 Msa0522520:CDS 60.0%
CTGAGCTGGCGGAGCTTCAT+TGG - chr3_4:75721374-75721393 Msa0522520:CDS 60.0%
AGCTGGCGGAGCTTCATTGG+AGG - chr3_4:75721377-75721396 Msa0522520:CDS 60.0%
GCCGCTCTCCAAGCTGAAGT+AGG + chr3_4:75721508-75721527 None:intergenic 60.0%
TTGGAGAGCGGCTGAGAAGC+TGG - chr3_4:75721516-75721535 Msa0522520:CDS 60.0%
GGCTGAGAAGCTGGCTAGGA+TGG - chr3_4:75721525-75721544 Msa0522520:CDS 60.0%
CGTGAAAACGGGGCAGGGGA+AGG - chr3_4:75719713-75719732 Msa0522520:CDS 65.0%
GCCCTGTCTGCAACGCCACA+TGG - chr3_4:75719817-75719836 Msa0522520:CDS 65.0%
! AAAGCACCACCGCCACTCCG+CGG - chr3_4:75720290-75720309 Msa0522520:CDS 65.0%
!! CAAGTGTGCTGGTTCCGCCG+CGG + chr3_4:75720310-75720329 None:intergenic 65.0%
!! TAGCACTGATCTCGGCGGCC+CGG + chr3_4:75720919-75720938 None:intergenic 65.0%
GAGCGGCTGAGAAGCTGGCT+AGG - chr3_4:75721521-75721540 Msa0522520:CDS 65.0%
CAAGGTGGTAGCAGACGGCG+CGG - chr3_4:75721743-75721762 Msa0522520:five_prime_UTR 65.0%
!! AGCACTGATCTCGGCGGCCC+GGG + chr3_4:75720918-75720937 None:intergenic 70.0%
CTGGTTCCGCCGCGGAGTGG+CGG + chr3_4:75720302-75720321 None:intergenic 75.0%
!! GTGCTGGTTCCGCCGCGGAG+TGG + chr3_4:75720305-75720324 None:intergenic 75.0%
! GCACCACCGCCACTCCGCGG+CGG - chr3_4:75720293-75720312 Msa0522520:CDS 80.0%
! GTTCCGCCGCGGAGTGGCGG+TGG + chr3_4:75720299-75720318 None:intergenic 80.0%
! CTGATCTCGGCGGCCCGGGC+CGG + chr3_4:75720914-75720933 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 75718832 75721931 75718832 ID=Msa0522520;Name=Msa0522520
chr3_4 mRNA 75718832 75721931 75718832 ID=Msa0522520-mRNA-1;Parent=Msa0522520;Name=Msa0522520-mRNA-1;_AED=0.00;_eAED=0.00;_QI=376|1|1|1|1|1|2|319|771
chr3_4 exon 75718832 75721076 75718832 ID=Msa0522520-mRNA-1:exon:21725;Parent=Msa0522520-mRNA-1
chr3_4 exon 75721166 75721931 75721166 ID=Msa0522520-mRNA-1:exon:21724;Parent=Msa0522520-mRNA-1
chr3_4 five_prime_UTR 75721556 75721931 75721556 ID=Msa0522520-mRNA-1:five_prime_utr;Parent=Msa0522520-mRNA-1
chr3_4 CDS 75721166 75721555 75721166 ID=Msa0522520-mRNA-1:cds;Parent=Msa0522520-mRNA-1
chr3_4 CDS 75719151 75721076 75719151 ID=Msa0522520-mRNA-1:cds;Parent=Msa0522520-mRNA-1
chr3_4 three_prime_UTR 75718832 75719150 75718832 ID=Msa0522520-mRNA-1:three_prime_utr;Parent=Msa0522520-mRNA-1
Gene Sequence

>Msa0522520

ATGAAAATGGGTACTGGGTGGAGAAGAGCCTTTTGTACACGAGACCCTGAATCTACCATACCAGATAATAAGAACAACAACAACAACGGAAGTCCTAATCCTAGTCCAAGAAGCTGTGCTAGATTGAGTTTTCTTTCTGGTGGTAGTAGTAATCCTTCAACTCCACGTTTGCACCAATCTAAATCTCAACCAGTTTCTAGTCCAAGTCTTCGTTGTCGAACAATAACTGAAGCAGCTTCTCAAATTACAAATGATAGCCCCAGATTTCAAAGTAGCAAAAGTAGCACCCCAAGATCAACAAAGTCTCCTAGAGTTAACTCAATATCAAATCCAACTTCTCCTCGCTCTCCTTTGAAGTTGTCCCTCTTCAAAAACAGCTTCAAATTCAGGAGTAGCTGTGGAATCTGTTTGAACAGCGTGAAAACGGGGCAGGGGAAGGCAATATACACAGCAGAATGTGCACATGCTTTTCATTTCCCTTGCATAGCAGCACATGTACGCAACCATGCTACCCTTGTTTGCCCTGTCTGCAACGCCACATGGAAAGACGTTCCTCTTCTAGCGGCGCACAAGAATCTCTCTTCTTCACAAGAAATTCCCAATGCAATTCCAAACCACAAAATACCTAATCCCTCTCCTGTTATCAGAACCAAGAACGTGGATCAGTCTCAACAAACTAATCATTCTGAATCAATAAGATCCTACGATGATGACGAGCCTCTACTCCTTTCTCCAACTTCTGGTGGTGGAAGGATTAACACTATCCCCGAAGCAGATGAAAATGCAGAGGAAGAAGATGATGACAACTGCGAGTTTCAAGGATTTTTCGTCAACGCAAAACCTACTTCCACCGCTAACAAAACGTACTCCGATGACATTCAAACCAGCGATGGAGTCGGTGATTCAAGAACTGTTCAGGTGAAGTTAATGCCAGAATGTGCCATCGTATCAGTTTCCAGAACACACGAAACATACGCTTTGGTTTTAAAAGTAAAAGCACCACCGCCACTCCGCGGCGGAACCAGCACACTTGATCCTTCACGGCGAGCTCCGATAGATCTTGTCACTGTTCTCGATGTTGGTGGAAGCATGTCCAGCGCAAAGCTTCACATGCTAAAACGCGCGATGCGTTTGGTTATTTCATCTCTTGGTCCTTCTGATAGGCTTTCAATTGTTGCTTTTTCGTCTGTTTCCAAACGGCTCTTACCGTTGAGAAGAATGACGGCTCAGGGTCAACGTTTGGCTCGGCGTATCGTTGACCGGCTTGTTACCGGTGAAGGTAACAGTGTCAGTGAAGCTTTGAGAAAAGCAACTACAGTTTTGGAGGATCGTAGAGAAAGAAACCCGGTTGCTAGCGTCATGCTGTTATCCGACGGTCAAGATGAAAAGGTTAATAACAGTAAGAATCAAAATCAAAATCAACGGAAGATGTACAACCACGCTTCTTCAACCCGGTTCGCTCATATTGAAATTCCGGTTCATGCGTTTGGGTTTGGTAGCAAAAGCGGTTATAGTCATGAACCGGGTGAGGATGCATTTGCAAAATGTGTTGGCGGTCTTTTGAGTGTTGTGGTTCAGGATTTAAGAGTTCAACTGGGCTTTCAATCTGATGATCCGGCCCGGGCCGCCGAGATCAGTGCTATATACTCTTGCAGTGGAAGACCCACGCTGTTGAGCTTAGGAGCCGTACGGCTCGGCGATTTATACGCTGAAGAAGAAAGAGAGCTTTTGGTGGAGATGAGAGTTCCAGCGTCAGCTTTAGGGTACGGGTCCCACCATGTGATGACGGTAAGGTGTCTTTATAAAGACCCCGCAAGTCAAGAGATAGTATACGGCAGAGAACAGGGACTTACAGTGCAACTACCTCAGAATCAATCTCAGAATATACGATCAACTGGGACAAGGATCGAAAGGTTGAGGAATCTTTTCATAACAACACGAGCCATAGCTGAGTCAAGGAGGCTTTTAGATCATAACAGTGACTTCACAAGTGCTCATCATTTACTTGCCTCGGCTCGTGCTCTTTTGATACAATCTGGCTCAACTTCAGCTGAGCAATATGTTCGTGGCTTGGAAGCTGAGCTGGCGGAGCTTCATTGGAGGAGACAGCAAGAACAGGTTCAAGTTGAGGCTCAGCAGCAACAAATGATGATGCAAAGAAGAAAAGGGTGCGAAAATATGGTGGATGAAAATGGTGAGCCACTTACACCTACTTCAGCTTGGAGAGCGGCTGAGAAGCTGGCTAGGATGGCTATGGTCAAGAAGTCTTTGAATAAAGTCAGTGACTTACATGGTTTCGAAAATGCTAGGTTTTGA
Protein sequence

>Msa0522520

MKMGTGWRRAFCTRDPESTIPDNKNNNNNGSPNPSPRSCARLSFLSGGSSNPSTPRLHQSKSQPVSSPSLRCRTITEAASQITNDSPRFQSSKSSTPRSTKSPRVNSISNPTSPRSPLKLSLFKNSFKFRSSCGICLNSVKTGQGKAIYTAECAHAFHFPCIAAHVRNHATLVCPVCNATWKDVPLLAAHKNLSSSQEIPNAIPNHKIPNPSPVIRTKNVDQSQQTNHSESIRSYDDDEPLLLSPTSGGGRINTIPEADENAEEEDDDNCEFQGFFVNAKPTSTANKTYSDDIQTSDGVGDSRTVQVKLMPECAIVSVSRTHETYALVLKVKAPPPLRGGTSTLDPSRRAPIDLVTVLDVGGSMSSAKLHMLKRAMRLVISSLGPSDRLSIVAFSSVSKRLLPLRRMTAQGQRLARRIVDRLVTGEGNSVSEALRKATTVLEDRRERNPVASVMLLSDGQDEKVNNSKNQNQNQRKMYNHASSTRFAHIEIPVHAFGFGSKSGYSHEPGEDAFAKCVGGLLSVVVQDLRVQLGFQSDDPARAAEISAIYSCSGRPTLLSLGAVRLGDLYAEEERELLVEMRVPASALGYGSHHVMTVRCLYKDPASQEIVYGREQGLTVQLPQNQSQNIRSTGTRIERLRNLFITTRAIAESRRLLDHNSDFTSAHHLLASARALLIQSGSTSAEQYVRGLEAELAELHWRRQQEQVQVEAQQQQMMMQRRKGCENMVDENGEPLTPTSAWRAAEKLARMAMVKKSLNKVSDLHGFENARF*