Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0531460 | XP_039689013.1 | 92.000 | 350 | 24 | 2 | 1 | 349 | 1 | 347 | 0.0 | 642 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0531460 | sp|Q54I39|IST1L_DICDI | 27.439 | 164 | 109 | 3 | 21 | 175 | 17 | 179 | 1.51e-14 | 77.4 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0531460 | A0A396HZ73 | 92.857 | 350 | 24 | 1 | 1 | 349 | 1 | 350 | 0.0 | 639 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0164400 | Msa0531460 | 0.803379 | 3.585862e-49 | -8.615850e-47 |
| Msa0194820 | Msa0531460 | 0.813288 | 2.761357e-51 | -8.615850e-47 |
| Msa0194840 | Msa0531460 | 0.811367 | 7.254711e-51 | -8.615850e-47 |
| Msa0531460 | Msa0588390 | 0.992330 | 1.202042e-192 | -8.615850e-47 |
| Msa0531460 | Msa0628670 | 0.838834 | 2.272595e-57 | -8.615850e-47 |
| Msa0531460 | Msa0796160 | 0.800225 | 1.592838e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0531460 | MtrunA17_Chr4g0004491 | 92.857 | 350 | 24 | 1 | 1 | 349 | 1 | 350 | 0.0 | 639 |
| Msa0531460 | MtrunA17_Chr2g0331801 | 50.329 | 304 | 134 | 6 | 4 | 297 | 1 | 297 | 6.40e-85 | 263 |
| Msa0531460 | MtrunA17_Chr8g0390551 | 48.705 | 193 | 97 | 2 | 4 | 195 | 1 | 192 | 1.26e-51 | 181 |
| Msa0531460 | MtrunA17_Chr6g0485461 | 42.035 | 226 | 121 | 2 | 4 | 228 | 57 | 273 | 3.45e-44 | 161 |
| Msa0531460 | MtrunA17_Chr8g0370931 | 36.126 | 191 | 111 | 4 | 15 | 194 | 29 | 219 | 6.40e-30 | 118 |
| Msa0531460 | MtrunA17_Chr1g0209771 | 31.156 | 199 | 126 | 4 | 13 | 200 | 13 | 211 | 3.99e-27 | 109 |
| Msa0531460 | MtrunA17_Chr6g0487991 | 32.973 | 185 | 111 | 4 | 4 | 178 | 1 | 182 | 7.69e-25 | 105 |
| Msa0531460 | MtrunA17_Chr2g0324121 | 33.333 | 174 | 106 | 3 | 4 | 167 | 1 | 174 | 2.87e-21 | 95.1 |
| Msa0531460 | MtrunA17_Chr6g0464791 | 32.447 | 188 | 116 | 4 | 15 | 191 | 15 | 202 | 6.49e-21 | 94.0 |
| Msa0531460 | MtrunA17_Chr4g0042021 | 30.726 | 179 | 112 | 3 | 4 | 173 | 1 | 176 | 6.36e-18 | 84.0 |
| Msa0531460 | MtrunA17_Chr7g0214961 | 26.286 | 175 | 117 | 3 | 8 | 171 | 3 | 176 | 3.95e-15 | 76.3 |
| Msa0531460 | MtrunA17_Chr1g0210081 | 24.074 | 162 | 112 | 3 | 21 | 171 | 22 | 183 | 1.31e-13 | 71.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0531460 | AT1G79910.1 | 40.876 | 274 | 160 | 2 | 4 | 276 | 1 | 273 | 2.72e-68 | 219 |
| Msa0531460 | AT1G52315.1 | 47.312 | 186 | 96 | 1 | 4 | 187 | 1 | 186 | 2.10e-53 | 179 |
| Msa0531460 | AT1G79910.3 | 37.963 | 216 | 132 | 2 | 62 | 276 | 4 | 218 | 1.27e-43 | 153 |
| Msa0531460 | AT1G79910.2 | 38.140 | 215 | 131 | 2 | 63 | 276 | 46 | 259 | 5.71e-43 | 152 |
| Msa0531460 | AT4G32350.1 | 41.935 | 186 | 107 | 1 | 4 | 188 | 1 | 186 | 6.33e-42 | 155 |
| Msa0531460 | AT4G32350.3 | 41.935 | 186 | 107 | 1 | 4 | 188 | 1 | 186 | 6.33e-42 | 155 |
| Msa0531460 | AT1G52315.2 | 50.000 | 120 | 58 | 1 | 70 | 187 | 17 | 136 | 5.50e-36 | 132 |
| Msa0531460 | AT4G35730.1 | 37.059 | 170 | 97 | 3 | 15 | 174 | 29 | 198 | 5.08e-28 | 114 |
| Msa0531460 | AT1G34220.2 | 33.673 | 196 | 119 | 4 | 15 | 199 | 15 | 210 | 3.61e-26 | 109 |
| Msa0531460 | AT1G25420.1 | 31.250 | 192 | 121 | 4 | 13 | 193 | 13 | 204 | 6.56e-24 | 100 |
| Msa0531460 | AT2G14830.1 | 33.333 | 186 | 114 | 3 | 4 | 179 | 1 | 186 | 2.38e-23 | 100 |
| Msa0531460 | AT4G32350.2 | 47.959 | 98 | 50 | 1 | 92 | 188 | 1 | 98 | 4.45e-23 | 100 |
| Msa0531460 | AT1G25420.4 | 31.638 | 177 | 110 | 4 | 28 | 193 | 1 | 177 | 3.01e-21 | 92.8 |
| Msa0531460 | AT1G34220.1 | 29.204 | 226 | 119 | 5 | 15 | 199 | 15 | 240 | 5.48e-21 | 94.7 |
| Msa0531460 | AT2G19710.1 | 29.255 | 188 | 120 | 4 | 1 | 178 | 1 | 185 | 1.35e-19 | 90.9 |
| Msa0531460 | AT4G29440.1 | 28.723 | 188 | 121 | 4 | 1 | 178 | 1 | 185 | 2.70e-19 | 89.7 |
| Msa0531460 | AT4G29440.2 | 31.013 | 158 | 98 | 4 | 31 | 178 | 1 | 157 | 5.02e-17 | 83.2 |
| Msa0531460 | AT1G25420.3 | 34.091 | 132 | 76 | 4 | 73 | 193 | 5 | 136 | 3.21e-16 | 77.8 |
| Msa0531460 | AT1G25420.2 | 34.091 | 132 | 76 | 4 | 73 | 193 | 5 | 136 | 3.21e-16 | 77.8 |
| Msa0531460 | AT1G13340.1 | 27.607 | 163 | 108 | 2 | 19 | 171 | 20 | 182 | 3.67e-16 | 79.3 |
Find 56 sgRNAs with CRISPR-Local
Find 129 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAATTCATTGAAAAGTTTAT+TGG | 0.167219 | 4_1:-5659480 | Msa0531460:CDS |
| CTGGAAGATCATAGAAAATT+AGG | 0.182221 | 4_1:-5657629 | Msa0531460:exon |
| ATGACATTGTTGAGCTTCTT+AGG | 0.233734 | 4_1:-5659823 | Msa0531460:CDS |
| AAAGATAAGAATAAGCATTT+AGG | 0.240134 | 4_1:+5660255 | None:intergenic |
| CTTATCTTCATTTCTCTTGT+AGG | 0.244844 | 4_1:+5659011 | None:intergenic |
| TTTACCTCTTTATTTATATA+TGG | 0.273867 | 4_1:+5659175 | None:intergenic |
| AGCCTAAGTTCTATTCAAAA+TGG | 0.300907 | 4_1:-5660070 | Msa0531460:intron |
| GTTCTAAGGTCACGCAATTC+TGG | 0.310148 | 4_1:+5659232 | None:intergenic |
| CATCATCACTACTACTATAT+TGG | 0.314596 | 4_1:+5658012 | None:intergenic |
| AAAGCTAGTTCAAGTTCATT+TGG | 0.341711 | 4_1:-5657945 | Msa0531460:CDS |
| TTTCTGTAAACAAAGTTCTA+AGG | 0.349616 | 4_1:+5659218 | None:intergenic |
| ATAGCTTCTTTGCATTCCTC+AGG | 0.361399 | 4_1:+5659295 | None:intergenic |
| CAATAATAGGGGACACAATC+TGG | 0.389420 | 4_1:-5658104 | Msa0531460:intron |
| TCATAATCAGGTAGAGTAGA+AGG | 0.391559 | 4_1:+5657681 | None:intergenic |
| GGGACACAATCTGGTGGAAC+TGG | 0.393720 | 4_1:-5658095 | Msa0531460:CDS |
| GACTGAAGGGCTTCTGTTTG+AGG | 0.394923 | 4_1:-5659523 | Msa0531460:CDS |
| ATTCTATGATACAAATGTTT+CGG | 0.401953 | 4_1:-5660103 | Msa0531460:exon |
| GCAATAAAATCATCATAATC+AGG | 0.413576 | 4_1:+5657669 | None:intergenic |
| CACAAGAGTTCTCTATAGAA+TGG | 0.414870 | 4_1:-5658971 | Msa0531460:CDS |
| TTACTTACTTCATATAATGA+TGG | 0.428302 | 4_1:+5658912 | None:intergenic |
| GATGTTTCCACTGATGTTGT+TGG | 0.439662 | 4_1:+5657750 | None:intergenic |
| ATGATTATGATGCATACAAA+AGG | 0.454117 | 4_1:-5659793 | Msa0531460:CDS |
| CTACAAGAGAAATGAAGATA+AGG | 0.485405 | 4_1:-5659010 | Msa0531460:CDS |
| TACTTACTTCATATAATGAT+GGG | 0.486277 | 4_1:+5658913 | None:intergenic |
| GCTCGTCTTAAAGCTTTCAC+TGG | 0.489333 | 4_1:-5657648 | Msa0531460:CDS |
| CTCTATAGAATGGGATGGGA+AGG | 0.509202 | 4_1:-5658961 | Msa0531460:CDS |
| CAATCTGGTGGAACTGGAGG+AGG | 0.518100 | 4_1:-5658089 | Msa0531460:CDS |
| ACAAGAGTTCTCTATAGAAT+GGG | 0.518976 | 4_1:-5658970 | Msa0531460:CDS |
| TTGCATTTGAAGCAGACTGA+AGG | 0.522662 | 4_1:-5659537 | Msa0531460:intron |
| TGAACCATATATAAATAAAG+AGG | 0.522792 | 4_1:-5659179 | Msa0531460:intron |
| AAAAGCGTATGCACAAATGT+GGG | 0.535251 | 4_1:-5657721 | Msa0531460:CDS |
| AATTAAGTAGATGCACCACT+AGG | 0.540525 | 4_1:+5657545 | None:intergenic |
| CAAAAGCGTATGCACAAATG+TGG | 0.540716 | 4_1:-5657722 | Msa0531460:CDS |
| TGCATTTGAAGCAGACTGAA+GGG | 0.541515 | 4_1:-5659536 | Msa0531460:intron |
| CACAAATGTGGGGTGGTGCA+TGG | 0.552893 | 4_1:-5657710 | Msa0531460:CDS |
| ATTGTTGAGCTTCTTAGGAG+TGG | 0.553106 | 4_1:-5659818 | Msa0531460:CDS |
| TAGCTTCTTTGCATTCCTCA+GGG | 0.557331 | 4_1:+5659296 | None:intergenic |
| TGATTATGATGCATACAAAA+GGG | 0.561869 | 4_1:-5659792 | Msa0531460:intron |
| GAGTTCTCTATAGAATGGGA+TGG | 0.565030 | 4_1:-5658966 | Msa0531460:CDS |
| GCTGCAGCATACATCAATGA+TGG | 0.570480 | 4_1:+5659271 | None:intergenic |
| CTATGATACAAATGTTTCGG+TGG | 0.572455 | 4_1:-5660100 | Msa0531460:CDS |
| TTTGTATCATAGAATATATG+AGG | 0.573235 | 4_1:+5660111 | None:intergenic |
| ACATCACCATCTCATCATCA+TGG | 0.573332 | 4_1:-5657972 | Msa0531460:CDS |
| TTTCTTCCATGATGATGAGA+TGG | 0.575256 | 4_1:+5657966 | None:intergenic |
| ACACAATCTGGTGGAACTGG+AGG | 0.583170 | 4_1:-5658092 | Msa0531460:CDS |
| TTGAAGAGTTAGTTAAGCAG+AGG | 0.591111 | 4_1:-5659440 | Msa0531460:intron |
| CAAGACGCGGTTAGAGACGA+TGG | 0.595738 | 4_1:-5659882 | Msa0531460:CDS |
| AGTTCTCTATAGAATGGGAT+GGG | 0.599718 | 4_1:-5658965 | Msa0531460:CDS |
| ATTAAGTAGATGCACCACTA+GGG | 0.618154 | 4_1:+5657546 | None:intergenic |
| AATGAAACCAACAACATCAG+TGG | 0.656362 | 4_1:-5657757 | Msa0531460:CDS |
| AAAGCGTATGCACAAATGTG+GGG | 0.677517 | 4_1:-5657720 | Msa0531460:CDS |
| TTAATTATTATACACCCTAG+TGG | 0.681350 | 4_1:-5657560 | Msa0531460:three_prime_UTR |
| ACATAAAATGTATCAAGACG+CGG | 0.698780 | 4_1:-5659895 | Msa0531460:CDS |
| TAATAGGGGACACAATCTGG+TGG | 0.702753 | 4_1:-5658101 | Msa0531460:intron |
| CTTATATAGTGAATGCCCTG+AGG | 0.705982 | 4_1:-5659311 | Msa0531460:intron |
| GCGTATGCACAAATGTGGGG+TGG | 0.730620 | 4_1:-5657717 | Msa0531460:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CTATTGAAAAAAAAATTATT+TGG | - | chr4_1:5657806-5657825 | Msa0531460:CDS | 10.0% |
| !! | AAAAATATGTTGAAATATTT+CGG | - | chr4_1:5659074-5659093 | Msa0531460:intron | 10.0% |
| !! | AGATTAACAAAATTATAAAA+TGG | - | chr4_1:5659245-5659264 | Msa0531460:CDS | 10.0% |
| !!! | GTTTATTCTAATTTATGTTT+TGG | - | chr4_1:5657834-5657853 | Msa0531460:CDS | 15.0% |
| !!! | TTTACCTCTTTATTTATATA+TGG | + | chr4_1:5658619-5658638 | None:intergenic | 15.0% |
| !!! | GAAATATTTCAACATATTTT+TGG | + | chr4_1:5659075-5659094 | None:intergenic | 15.0% |
| !! | TGATATTTGATTAATTTCAA+TGG | - | chr4_1:5659359-5659378 | Msa0531460:intron | 15.0% |
| !! | GATATTTGATTAATTTCAAT+GGG | - | chr4_1:5659360-5659379 | Msa0531460:intron | 15.0% |
| !! | AAAGATAAGAATAAGCATTT+AGG | + | chr4_1:5657539-5657558 | None:intergenic | 20.0% |
| !! | TTTGTATCATAGAATATATG+AGG | + | chr4_1:5657683-5657702 | None:intergenic | 20.0% |
| !! | ATTCTATGATACAAATGTTT+CGG | - | chr4_1:5657688-5657707 | Msa0531460:CDS | 20.0% |
| !!! | AAAATCACAATTTTAGTTAC+TGG | + | chr4_1:5657763-5657782 | None:intergenic | 20.0% |
| !! | TACTTGTAAGCAAAATTTAA+TGG | + | chr4_1:5658043-5658062 | None:intergenic | 20.0% |
| !! | CATGACAATAAATTAAACAT+TGG | - | chr4_1:5658088-5658107 | Msa0531460:CDS | 20.0% |
| !! | GAATTCATTGAAAAGTTTAT+TGG | - | chr4_1:5658311-5658330 | Msa0531460:intron | 20.0% |
| !! | ATTTAGTGCATATACATATA+AGG | + | chr4_1:5658386-5658405 | None:intergenic | 20.0% |
| !!! | ACTTTGTTTACAGAAAAATA+TGG | - | chr4_1:5658580-5658599 | Msa0531460:intron | 20.0% |
| !! | TGAACCATATATAAATAAAG+AGG | - | chr4_1:5658612-5658631 | Msa0531460:intron | 20.0% |
| !! | TACTTACTTCATATAATGAT+GGG | + | chr4_1:5658881-5658900 | None:intergenic | 20.0% |
| !! | TTACTTACTTCATATAATGA+TGG | + | chr4_1:5658882-5658901 | None:intergenic | 20.0% |
| !! | TATAAAATGGTTCATGAAAT+TGG | - | chr4_1:5659258-5659277 | Msa0531460:CDS | 20.0% |
| !! | CTTTATAGGACTAATATAAT+TGG | + | chr4_1:5659289-5659308 | None:intergenic | 20.0% |
| !! | GTATGTAAAAGAATCTTTAT+AGG | + | chr4_1:5659303-5659322 | None:intergenic | 20.0% |
| !! | TTTACATACTAATGTGATTA+TGG | - | chr4_1:5659314-5659333 | Msa0531460:intron | 20.0% |
| !! | TTACATACTAATGTGATTAT+GGG | - | chr4_1:5659315-5659334 | Msa0531460:intron | 20.0% |
| !!! | TGTTGGATATTTTAAAAGTT+GGG | + | chr4_1:5659427-5659446 | None:intergenic | 20.0% |
| !!! | ATGTTGGATATTTTAAAAGT+TGG | + | chr4_1:5659428-5659447 | None:intergenic | 20.0% |
| !!! | GAATCAATATTTTTCTCTAT+TGG | + | chr4_1:5660008-5660027 | None:intergenic | 20.0% |
| !!! | ACATTTCACTTTTAGTCAAT+AGG | - | chr4_1:5657653-5657672 | Msa0531460:CDS | 25.0% |
| !!! | CATTTCACTTTTAGTCAATA+GGG | - | chr4_1:5657654-5657673 | Msa0531460:CDS | 25.0% |
| !! | TACCATTTTGAATAGAACTT+AGG | + | chr4_1:5657726-5657745 | None:intergenic | 25.0% |
| !!! | TCTAATTTATGTTTTGGCTT+TGG | - | chr4_1:5657840-5657859 | Msa0531460:CDS | 25.0% |
| ! | ATGATTATGATGCATACAAA+AGG | - | chr4_1:5657998-5658017 | Msa0531460:CDS | 25.0% |
| ! | TGATTATGATGCATACAAAA+GGG | - | chr4_1:5657999-5658018 | Msa0531460:CDS | 25.0% |
| ! | TTTCTGTAAACAAAGTTCTA+AGG | + | chr4_1:5658576-5658595 | None:intergenic | 25.0% |
| !!! | TAGTCACAATGTTTTTTTGA+AGG | + | chr4_1:5658926-5658945 | None:intergenic | 25.0% |
| !!! | ATATTTTTGGATGTTTTACG+TGG | + | chr4_1:5659062-5659081 | None:intergenic | 25.0% |
| !!! | TTTTTTTGACTCTTCAATCA+AGG | + | chr4_1:5659215-5659234 | None:intergenic | 25.0% |
| !! | AGTTACCTTATTCACAATTT+TGG | - | chr4_1:5659398-5659417 | Msa0531460:intron | 25.0% |
| ! | AACAACTCATTTCTATGTTT+GGG | + | chr4_1:5659558-5659577 | None:intergenic | 25.0% |
| ! | TAACAACTCATTTCTATGTT+TGG | + | chr4_1:5659559-5659578 | None:intergenic | 25.0% |
| ! | TACATAAACTGACCAATAAT+AGG | - | chr4_1:5659674-5659693 | Msa0531460:intron | 25.0% |
| ! | ACATAAACTGACCAATAATA+GGG | - | chr4_1:5659675-5659694 | Msa0531460:intron | 25.0% |
| !!! | TTTTTTGTGGTTTGAAGTAA+GGG | + | chr4_1:5659938-5659957 | None:intergenic | 25.0% |
| !!! | CCTTTGTTATTAGTTTTTTG+TGG | + | chr4_1:5659951-5659970 | None:intergenic | 25.0% |
| ! | CCACAAAAAACTAATAACAA+AGG | - | chr4_1:5659948-5659967 | Msa0531460:intron | 25.0% |
| !!! | TTCAGAATCTGATGTTTTTT+TGG | + | chr4_1:5659985-5660004 | None:intergenic | 25.0% |
| !!! | GAGAAAAATATTGATTCATG+TGG | - | chr4_1:5660011-5660030 | Msa0531460:intron | 25.0% |
| ! | GCAATAAAATCATCATAATC+AGG | + | chr4_1:5660125-5660144 | None:intergenic | 25.0% |
| ! | TTAATTATTATACACCCTAG+TGG | - | chr4_1:5660231-5660250 | Msa0531460:five_prime_UTR | 25.0% |
| !! | GATGTTGTTGGATATTTTAG+AGG | + | chr4_1:5657625-5657644 | None:intergenic | 30.0% |
| ! | ATGTCTTTTCTTGATGTTGT+TGG | + | chr4_1:5657637-5657656 | None:intergenic | 30.0% |
| AGCCTAAGTTCTATTCAAAA+TGG | - | chr4_1:5657721-5657740 | Msa0531460:CDS | 30.0% | |
| ACATAAAATGTATCAAGACG+CGG | - | chr4_1:5657896-5657915 | Msa0531460:CDS | 30.0% | |
| ! | CTTTTCAATGAATTCATAGC+AGG | + | chr4_1:5658306-5658325 | None:intergenic | 30.0% |
| CTTATCTTCATTTCTCTTGT+AGG | + | chr4_1:5658783-5658802 | None:intergenic | 30.0% | |
| CTACAAGAGAAATGAAGATA+AGG | - | chr4_1:5658781-5658800 | Msa0531460:intron | 30.0% | |
| ACAAGAGTTCTCTATAGAAT+GGG | - | chr4_1:5658821-5658840 | Msa0531460:intron | 30.0% | |
| !!! | TCGGATTTTAGAATCCAAAT+AGG | - | chr4_1:5659093-5659112 | Msa0531460:intron | 30.0% |
| !!! | CGGATTTTAGAATCCAAATA+GGG | - | chr4_1:5659094-5659113 | Msa0531460:intron | 30.0% |
| ! | TCATATGGTGGAAAAAGTAA+TGG | - | chr4_1:5659129-5659148 | Msa0531460:intron | 30.0% |
| GGATACCAAAATTGTGAATA+AGG | + | chr4_1:5659406-5659425 | None:intergenic | 30.0% | |
| AAAATCAACCTCAAATGCAT+AGG | + | chr4_1:5659459-5659478 | None:intergenic | 30.0% | |
| CATAAACTGACCAATAATAG+GGG | - | chr4_1:5659676-5659695 | Msa0531460:intron | 30.0% | |
| CATCATCACTACTACTATAT+TGG | + | chr4_1:5659782-5659801 | None:intergenic | 30.0% | |
| ! | AAAGCTAGTTCAAGTTCATT+TGG | - | chr4_1:5659846-5659865 | Msa0531460:CDS | 30.0% |
| GGAATTAGCAAAAATGAACT+TGG | + | chr4_1:5659912-5659931 | None:intergenic | 30.0% | |
| !! | TTTTTGTGGTTTGAAGTAAG+GGG | + | chr4_1:5659937-5659956 | None:intergenic | 30.0% |
| !!! | GTTTTTTGTGGTTTGAAGTA+AGG | + | chr4_1:5659939-5659958 | None:intergenic | 30.0% |
| CTGGAAGATCATAGAAAATT+AGG | - | chr4_1:5660162-5660181 | Msa0531460:five_prime_UTR | 30.0% | |
| !! | ATATTTTAGAGGCGATGTTG+TGG | + | chr4_1:5657614-5657633 | None:intergenic | 35.0% |
| CTATGATACAAATGTTTCGG+TGG | - | chr4_1:5657691-5657710 | Msa0531460:CDS | 35.0% | |
| ATGACATTGTTGAGCTTCTT+AGG | - | chr4_1:5657968-5657987 | Msa0531460:CDS | 35.0% | |
| TTGAAGAGTTAGTTAAGCAG+AGG | - | chr4_1:5658351-5658370 | Msa0531460:intron | 35.0% | |
| TTGAGTATACTCTACAAGAG+TGG | + | chr4_1:5658651-5658670 | None:intergenic | 35.0% | |
| CTCAACTTCTCAACAAACTA+AGG | + | chr4_1:5658753-5658772 | None:intergenic | 35.0% | |
| CACAAGAGTTCTCTATAGAA+TGG | - | chr4_1:5658820-5658839 | Msa0531460:intron | 35.0% | |
| AGTTCTCTATAGAATGGGAT+GGG | - | chr4_1:5658826-5658845 | Msa0531460:intron | 35.0% | |
| TATATATATCCAAGCGAACG+AGG | - | chr4_1:5658950-5658969 | Msa0531460:CDS | 35.0% | |
| ! | TACGTGGTTTTTATGCAGTT+CGG | + | chr4_1:5659046-5659065 | None:intergenic | 35.0% |
| ATTGAGTGATTCACCTACTT+TGG | - | chr4_1:5659188-5659207 | Msa0531460:CDS | 35.0% | |
| ! | GTGCTTTGTCTCATTTTGTT+TGG | + | chr4_1:5659760-5659779 | None:intergenic | 35.0% |
| ! | TTTCTTCCATGATGATGAGA+TGG | + | chr4_1:5659828-5659847 | None:intergenic | 35.0% |
| !! | TTTTGTGGTTTGAAGTAAGG+GGG | + | chr4_1:5659936-5659955 | None:intergenic | 35.0% |
| AATGAAACCAACAACATCAG+TGG | - | chr4_1:5660034-5660053 | Msa0531460:intron | 35.0% | |
| ! | AAAAGCGTATGCACAAATGT+GGG | - | chr4_1:5660070-5660089 | Msa0531460:intron | 35.0% |
| TCATAATCAGGTAGAGTAGA+AGG | + | chr4_1:5660113-5660132 | None:intergenic | 35.0% | |
| ATTAAGTAGATGCACCACTA+GGG | + | chr4_1:5660248-5660267 | None:intergenic | 35.0% | |
| AATTAAGTAGATGCACCACT+AGG | + | chr4_1:5660249-5660268 | None:intergenic | 35.0% | |
| ATTGTTGAGCTTCTTAGGAG+TGG | - | chr4_1:5657973-5657992 | Msa0531460:CDS | 40.0% | |
| ACATGTGATACGTGTTGTGT+CGG | - | chr4_1:5658148-5658167 | Msa0531460:intron | 40.0% | |
| ! | TTGCATTTGAAGCAGACTGA+AGG | - | chr4_1:5658254-5658273 | Msa0531460:intron | 40.0% |
| ! | TGCATTTGAAGCAGACTGAA+GGG | - | chr4_1:5658255-5658274 | Msa0531460:intron | 40.0% |
| CTTATATAGTGAATGCCCTG+AGG | - | chr4_1:5658480-5658499 | Msa0531460:intron | 40.0% | |
| TAGCTTCTTTGCATTCCTCA+GGG | + | chr4_1:5658498-5658517 | None:intergenic | 40.0% | |
| ATAGCTTCTTTGCATTCCTC+AGG | + | chr4_1:5658499-5658518 | None:intergenic | 40.0% | |
| ACTCTACAAGAGTGGATCAT+TGG | + | chr4_1:5658643-5658662 | None:intergenic | 40.0% | |
| GAGTTCTCTATAGAATGGGA+TGG | - | chr4_1:5658825-5658844 | Msa0531460:intron | 40.0% | |
| GGATTAAATCCGAACTCCAT+AGG | + | chr4_1:5659025-5659044 | None:intergenic | 40.0% | |
| CTTCAATCAAGGACCAAAGT+AGG | + | chr4_1:5659204-5659223 | None:intergenic | 40.0% | |
| TGCATAGGTGGAGAAAATGT+TGG | + | chr4_1:5659444-5659463 | None:intergenic | 40.0% | |
| TTTCTCCACCTATGCATTTG+AGG | - | chr4_1:5659448-5659467 | Msa0531460:CDS | 40.0% | |
| ATCAACCTCAAATGCATAGG+TGG | + | chr4_1:5659456-5659475 | None:intergenic | 40.0% | |
| CAGATTGTGTCCCCTATTAT+TGG | + | chr4_1:5659689-5659708 | None:intergenic | 40.0% | |
| CAATAATAGGGGACACAATC+TGG | - | chr4_1:5659687-5659706 | Msa0531460:intron | 40.0% | |
| ACATCACCATCTCATCATCA+TGG | - | chr4_1:5659819-5659838 | Msa0531460:CDS | 40.0% | |
| GATGTTTCCACTGATGTTGT+TGG | + | chr4_1:5660044-5660063 | None:intergenic | 40.0% | |
| ! | CAAAAGCGTATGCACAAATG+TGG | - | chr4_1:5660069-5660088 | Msa0531460:intron | 40.0% |
| AAAGCGTATGCACAAATGTG+GGG | - | chr4_1:5660071-5660090 | Msa0531460:CDS | 40.0% | |
| GCTGCAGCATACATCAATGA+TGG | + | chr4_1:5658523-5658542 | None:intergenic | 45.0% | |
| !! | GTTCTAAGGTCACGCAATTC+TGG | + | chr4_1:5658562-5658581 | None:intergenic | 45.0% |
| CTCTATAGAATGGGATGGGA+AGG | - | chr4_1:5658830-5658849 | Msa0531460:intron | 45.0% | |
| AAACAAATGCCTCGTTCGCT+TGG | + | chr4_1:5658962-5658981 | None:intergenic | 45.0% | |
| TAATAGGGGACACAATCTGG+TGG | - | chr4_1:5659690-5659709 | Msa0531460:intron | 45.0% | |
| GCTCGTCTTAAAGCTTTCAC+TGG | - | chr4_1:5660143-5660162 | Msa0531460:five_prime_UTR | 45.0% | |
| ! | GATACGTGTTGTGTCGGTGT+TGG | - | chr4_1:5658154-5658173 | Msa0531460:intron | 50.0% |
| GACTGAAGGGCTTCTGTTTG+AGG | - | chr4_1:5658268-5658287 | Msa0531460:intron | 50.0% | |
| TGAGCTTCTCACGAGTCCTA+TGG | - | chr4_1:5659006-5659025 | Msa0531460:CDS | 50.0% | |
| CTCACGAGTCCTATGGAGTT+CGG | - | chr4_1:5659013-5659032 | Msa0531460:CDS | 50.0% | |
| AGCTTCTTGCACGCCCTATT+TGG | + | chr4_1:5659110-5659129 | None:intergenic | 50.0% | |
| CGTGCAAGAAGCTCATATGG+TGG | - | chr4_1:5659117-5659136 | Msa0531460:intron | 50.0% | |
| ACACAATCTGGTGGAACTGG+AGG | - | chr4_1:5659699-5659718 | Msa0531460:intron | 50.0% | |
| ! | TGTGGTTTGAAGTAAGGGGG+TGG | + | chr4_1:5659933-5659952 | None:intergenic | 50.0% |
| CAAGACGCGGTTAGAGACGA+TGG | - | chr4_1:5657909-5657928 | Msa0531460:CDS | 55.0% | |
| GGGCGTGCAAGAAGCTCATA+TGG | - | chr4_1:5659114-5659133 | Msa0531460:intron | 55.0% | |
| GGGACACAATCTGGTGGAAC+TGG | - | chr4_1:5659696-5659715 | Msa0531460:intron | 55.0% | |
| CAATCTGGTGGAACTGGAGG+AGG | - | chr4_1:5659702-5659721 | Msa0531460:intron | 55.0% | |
| GCGTATGCACAAATGTGGGG+TGG | - | chr4_1:5660074-5660093 | Msa0531460:CDS | 55.0% | |
| CACAAATGTGGGGTGGTGCA+TGG | - | chr4_1:5660081-5660100 | Msa0531460:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_1 | gene | 5657514 | 5660299 | 5657514 | ID=Msa0531460;Name=Msa0531460 |
| chr4_1 | mRNA | 5657514 | 5660299 | 5657514 | ID=Msa0531460-mRNA-1;Parent=Msa0531460;Name=Msa0531460-mRNA-1;_AED=0.06;_eAED=0.06;_QI=179|1|1|1|0.8|0.66|6|124|349 |
| chr4_1 | exon | 5657514 | 5658117 | 5657514 | ID=Msa0531460-mRNA-1:exon:1816;Parent=Msa0531460-mRNA-1 |
| chr4_1 | exon | 5658920 | 5659057 | 5658920 | ID=Msa0531460-mRNA-1:exon:1815;Parent=Msa0531460-mRNA-1 |
| chr4_1 | exon | 5659180 | 5659324 | 5659180 | ID=Msa0531460-mRNA-1:exon:1814;Parent=Msa0531460-mRNA-1 |
| chr4_1 | exon | 5659441 | 5659544 | 5659441 | ID=Msa0531460-mRNA-1:exon:1813;Parent=Msa0531460-mRNA-1 |
| chr4_1 | exon | 5659793 | 5659925 | 5659793 | ID=Msa0531460-mRNA-1:exon:1812;Parent=Msa0531460-mRNA-1 |
| chr4_1 | exon | 5660071 | 5660299 | 5660071 | ID=Msa0531460-mRNA-1:exon:1811;Parent=Msa0531460-mRNA-1 |
| chr4_1 | five_prime_UTR | 5660121 | 5660299 | 5660121 | ID=Msa0531460-mRNA-1:five_prime_utr;Parent=Msa0531460-mRNA-1 |
| chr4_1 | CDS | 5660071 | 5660120 | 5660071 | ID=Msa0531460-mRNA-1:cds;Parent=Msa0531460-mRNA-1 |
| chr4_1 | CDS | 5659793 | 5659925 | 5659793 | ID=Msa0531460-mRNA-1:cds;Parent=Msa0531460-mRNA-1 |
| chr4_1 | CDS | 5659441 | 5659544 | 5659441 | ID=Msa0531460-mRNA-1:cds;Parent=Msa0531460-mRNA-1 |
| chr4_1 | CDS | 5659180 | 5659324 | 5659180 | ID=Msa0531460-mRNA-1:cds;Parent=Msa0531460-mRNA-1 |
| chr4_1 | CDS | 5658920 | 5659057 | 5658920 | ID=Msa0531460-mRNA-1:cds;Parent=Msa0531460-mRNA-1 |
| chr4_1 | CDS | 5657638 | 5658117 | 5657638 | ID=Msa0531460-mRNA-1:cds;Parent=Msa0531460-mRNA-1 |
| chr4_1 | three_prime_UTR | 5657514 | 5657637 | 5657514 | ID=Msa0531460-mRNA-1:three_prime_utr;Parent=Msa0531460-mRNA-1 |
| Gene Sequence |
| Protein sequence |
