AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0164400

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0164400 MtrunA17_Chr2g0285961 90.421 261 25 0 1 261 1 261 2.00e-175 483
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0164400 AT2G34530.1 38.424 203 111 5 70 259 67 268 1.98e-30 115
Msa0164400 AT2G34530.4 38.424 203 111 5 70 259 77 278 2.43e-30 115
Msa0164400 AT2G34530.3 38.424 203 111 5 70 259 111 312 4.28e-30 115
Msa0164400 AT2G34530.5 43.885 139 75 2 70 206 77 214 2.18e-25 100
Msa0164400 AT2G34530.2 43.885 139 75 2 70 206 67 204 4.85e-25 100

Find 64 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTCTCTATCTTTCGACTTA+TGG 0.180793 1_4:+43813533 Msa0164400:CDS
CTCTTTCAATCTATCCAATA+TGG 0.186585 1_4:-43813651 None:intergenic
TCTTGATTTAATAAAGATTC+AGG 0.264321 1_4:+43813754 Msa0164400:CDS
GTCTCTATCTTTCGACTTAT+GGG 0.294022 1_4:+43813534 Msa0164400:CDS
TAAATAGTTCTTTCTCTTTG+TGG 0.306506 1_4:+43813343 Msa0164400:five_prime_UTR
AGTGCAATACAAGAATTATC+AGG 0.313725 1_4:+43814957 Msa0164400:CDS
GCCGTTGTTATTGTCATCCT+TGG 0.313936 1_4:+43813509 Msa0164400:CDS
AGTAGAAATGCTCATCTATA+AGG 0.325228 1_4:+43814029 Msa0164400:CDS
CACTTGTGTGCTAAATGATT+TGG 0.330580 1_4:+43813306 None:intergenic
TGAGCTTGAGCTTCGACTTT+CGG 0.331242 1_4:-43813562 None:intergenic
ATCATTATATGTTAGATAAT+AGG 0.341955 1_4:+43815010 Msa0164400:three_prime_UTR
GAATTCATTCCAACATTCAT+TGG 0.362634 1_4:-43814862 None:intergenic
GAACTTATAAAGAGAAAATT+CGG 0.363931 1_4:-43814910 None:intergenic
TCATAGTGGAAAACCCATAT+TGG 0.366180 1_4:+43813637 Msa0164400:CDS
TTTCCGTGTTTCCCTTCATT+TGG 0.370913 1_4:-43814836 None:intergenic
AGATATGTTGCAGCAAGCTT+TGG 0.373596 1_4:+43813891 Msa0164400:intron
TTCATGATAATTTGAAGAAA+GGG 0.388116 1_4:-43813420 None:intergenic
CCTCCACTAGTTAAAATTCC+TGG 0.388708 1_4:-43815295 None:intergenic
ACGGTCAAAGTGTCCGGACT+TGG 0.391154 1_4:-43813588 None:intergenic
TCTTTCAATCTATCCAATAT+GGG 0.393501 1_4:-43813650 None:intergenic
CTTCATGATAATTTGAAGAA+AGG 0.402053 1_4:-43813421 None:intergenic
AGATTCTGCGGTGGAAATAC+TGG 0.405408 1_4:+43813939 Msa0164400:CDS
CTTCAATTGGCAGGGAGAGC+TGG 0.406149 1_4:+43814438 Msa0164400:intron
CTCAAGCTCACGCCCAAGTC+CGG 0.417252 1_4:+43813575 Msa0164400:CDS
CAAACTCGTACAAATATTAG+AGG 0.428879 1_4:-43815258 None:intergenic
CCGTTGTTATTGTCATCCTT+GGG 0.433089 1_4:+43813510 Msa0164400:CDS
GGCAATTATATACCAAATGA+AGG 0.439320 1_4:+43814824 Msa0164400:intron
TTTCAAGCATGTACGGTTCA+AGG 0.445106 1_4:-43814467 None:intergenic
AGTAATTTATAATTGAAGTC+AGG 0.472849 1_4:+43815167 Msa0164400:three_prime_UTR
CATATTGGATAGATTGAAAG+AGG 0.477246 1_4:+43813652 Msa0164400:CDS
CCGGACACTTTGACCGTACA+AGG 0.481049 1_4:+43813594 Msa0164400:CDS
TGCTCGACGCCCACTGTACA+AGG 0.484519 1_4:+43814507 Msa0164400:CDS
GCAGCAAGCTTTGGAAAAGC+TGG 0.488777 1_4:+43813900 Msa0164400:intron
ACTGGAAAATGCCGTAAAGA+AGG 0.511553 1_4:+43813957 Msa0164400:CDS
TAACGATCCATGACCTTGTA+CGG 0.512166 1_4:-43813607 None:intergenic
ACAAGAATTATCAGGGAGGA+AGG 0.520561 1_4:+43814965 Msa0164400:CDS
TTTGACACAGTAGCGGAGCC+AGG 0.520705 1_4:+43815277 Msa0164400:three_prime_UTR
CCTTGTACGGTCAAAGTGTC+CGG 0.522757 1_4:-43813594 None:intergenic
CGGTCAAAGTGTCCGGACTT+GGG 0.540111 1_4:-43813587 None:intergenic
AATCATCTTTCAAGCATGTA+CGG 0.563613 1_4:-43814474 None:intergenic
TAAAGAGAAAATTCGGTGCA+AGG 0.565220 1_4:-43814903 None:intergenic
ACTTTGACCGTACAAGGTCA+TGG 0.569622 1_4:+43813600 Msa0164400:CDS
GTGCAATACAAGAATTATCA+GGG 0.569824 1_4:+43814958 Msa0164400:CDS
AAATATCAAGATGGCAACAC+TGG 0.574878 1_4:+43813487 Msa0164400:CDS
CGACTCAAGTATGAATCACC+TGG 0.592794 1_4:+43813376 Msa0164400:exon
GCAATTATATACCAAATGAA+GGG 0.605208 1_4:+43814825 Msa0164400:CDS
CAAGAATTATCAGGGAGGAA+GGG 0.609044 1_4:+43814966 Msa0164400:CDS
TTGGAAAAGCTGGAAATGAG+TGG 0.622925 1_4:+43813910 Msa0164400:CDS
TGACTATAATGTTTACTGTG+AGG 0.625488 1_4:+43815072 Msa0164400:three_prime_UTR
GTATGAGTTAAATATCAAGA+TGG 0.627283 1_4:+43813478 Msa0164400:CDS
GAACGTGTACCTTGTACAGT+GGG 0.633465 1_4:-43814516 None:intergenic
GGAACGTGTACCTTGTACAG+TGG 0.634635 1_4:-43814517 None:intergenic
ATACCAAATGAAGGGAAACA+CGG 0.638175 1_4:+43814833 Msa0164400:CDS
AAGGAGGATCTTGTACCGCA+AGG 0.649015 1_4:-43814884 None:intergenic
GTACGAGTTTGACACAGTAG+CGG 0.654783 1_4:+43815270 Msa0164400:three_prime_UTR
ATCGTTAGAGAAATTCATAG+TGG 0.664835 1_4:+43813623 Msa0164400:CDS
TCGAAAGATAGAGACTCCCA+AGG 0.672047 1_4:-43813526 None:intergenic
CCCAAGGATGACAATAACAA+CGG 0.691024 1_4:-43813510 None:intergenic
TGGAAGTGAAGATTCTGCGG+TGG 0.692864 1_4:+43813930 Msa0164400:CDS
AGAGAAAATTCGGTGCAAGG+AGG 0.706718 1_4:-43814900 None:intergenic
GAGTGGAAGTGAAGATTCTG+CGG 0.718050 1_4:+43813927 Msa0164400:CDS
TGTGTGCTAAATGATTTGGT+AGG 0.727793 1_4:+43813310 Msa0164400:five_prime_UTR
ATGTTGGAATGAATTCCTTG+CGG 0.756613 1_4:+43814869 Msa0164400:CDS
CAATACAAGAATTATCAGGG+AGG 0.773135 1_4:+43814961 Msa0164400:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAAGTTTATTTACATTTT+TGG + chr1_4:43814104-43814123 Msa0164400:intron 10.0%
!!! TTTAAGTTTATTTACATTTT+TGG + chr1_4:43814104-43814123 Msa0164400:intron 10.0%
!!! TAATTTAGGTATATATGTTT+TGG + chr1_4:43814131-43814150 Msa0164400:intron 15.0%
!!! AATGAACTTTTATAATCTAA+TGG + chr1_4:43814273-43814292 Msa0164400:intron 15.0%
!! TTATAAGTTCAAAAAAAATG+TGG + chr1_4:43814923-43814942 Msa0164400:CDS 15.0%
!! ATCATTATATGTTAGATAAT+AGG + chr1_4:43815010-43815029 Msa0164400:three_prime_UTR 15.0%
!!! TAATTTAGGTATATATGTTT+TGG + chr1_4:43814131-43814150 Msa0164400:intron 15.0%
!!! AATGAACTTTTATAATCTAA+TGG + chr1_4:43814273-43814292 Msa0164400:intron 15.0%
!! TTATAAGTTCAAAAAAAATG+TGG + chr1_4:43814923-43814942 Msa0164400:CDS 15.0%
!! ATCATTATATGTTAGATAAT+AGG + chr1_4:43815010-43815029 Msa0164400:three_prime_UTR 15.0%
!! TTCATGATAATTTGAAGAAA+GGG - chr1_4:43813423-43813442 None:intergenic 20.0%
!! TCTTGATTTAATAAAGATTC+AGG + chr1_4:43813754-43813773 Msa0164400:CDS 20.0%
!! AACAATAAATCTTAGTATGA+AGG - chr1_4:43813798-43813817 None:intergenic 20.0%
!! AACATATATACCTAAATTAG+AGG - chr1_4:43814130-43814149 None:intergenic 20.0%
!! ACGAAATTAATGTTAAATCT+TGG + chr1_4:43814302-43814321 Msa0164400:intron 20.0%
!!! CATTGAAATTTCAAGTTATT+AGG - chr1_4:43814328-43814347 None:intergenic 20.0%
!! AATGACCTAAAACAAATTAT+TGG + chr1_4:43814344-43814363 Msa0164400:intron 20.0%
!! TATGAAAAGAAAACTTCAAT+TGG + chr1_4:43814425-43814444 Msa0164400:intron 20.0%
!!! TATTTGATAATTTAGATGAG+AGG - chr1_4:43814563-43814582 None:intergenic 20.0%
!!! TTGATTCTTTCTTTTTTTAC+AGG + chr1_4:43814803-43814822 Msa0164400:intron 20.0%
!! GAACTTATAAAGAGAAAATT+CGG - chr1_4:43814913-43814932 None:intergenic 20.0%
!! AGTAATTTATAATTGAAGTC+AGG + chr1_4:43815167-43815186 Msa0164400:three_prime_UTR 20.0%
!! TTCATGATAATTTGAAGAAA+GGG - chr1_4:43813423-43813442 None:intergenic 20.0%
!! TCTTGATTTAATAAAGATTC+AGG + chr1_4:43813754-43813773 Msa0164400:CDS 20.0%
!! AACAATAAATCTTAGTATGA+AGG - chr1_4:43813798-43813817 None:intergenic 20.0%
!! AACATATATACCTAAATTAG+AGG - chr1_4:43814130-43814149 None:intergenic 20.0%
!! ACGAAATTAATGTTAAATCT+TGG + chr1_4:43814302-43814321 Msa0164400:intron 20.0%
!!! CATTGAAATTTCAAGTTATT+AGG - chr1_4:43814328-43814347 None:intergenic 20.0%
!! AATGACCTAAAACAAATTAT+TGG + chr1_4:43814344-43814363 Msa0164400:intron 20.0%
!! TATGAAAAGAAAACTTCAAT+TGG + chr1_4:43814425-43814444 Msa0164400:intron 20.0%
!!! TATTTGATAATTTAGATGAG+AGG - chr1_4:43814563-43814582 None:intergenic 20.0%
!!! TTGATTCTTTCTTTTTTTAC+AGG + chr1_4:43814803-43814822 Msa0164400:intron 20.0%
!! GAACTTATAAAGAGAAAATT+CGG - chr1_4:43814913-43814932 None:intergenic 20.0%
!! AGTAATTTATAATTGAAGTC+AGG + chr1_4:43815167-43815186 Msa0164400:three_prime_UTR 20.0%
! TAAATAGTTCTTTCTCTTTG+TGG + chr1_4:43813343-43813362 Msa0164400:five_prime_UTR 25.0%
! CTTCATGATAATTTGAAGAA+AGG - chr1_4:43813424-43813443 None:intergenic 25.0%
! GTATGAGTTAAATATCAAGA+TGG + chr1_4:43813478-43813497 Msa0164400:CDS 25.0%
! TCTTTCAATCTATCCAATAT+GGG - chr1_4:43813653-43813672 None:intergenic 25.0%
!!! TTAGTTTGTTTTAATGTTGC+AGG - chr1_4:43813701-43813720 None:intergenic 25.0%
!!! ACAAACTAAGAAAGTTTTGA+AGG + chr1_4:43813712-43813731 Msa0164400:CDS 25.0%
! AACAATTATCTAACGTACTT+AGG - chr1_4:43813829-43813848 None:intergenic 25.0%
!!! ATCGTCCAATAATTTGTTTT+AGG - chr1_4:43814352-43814371 None:intergenic 25.0%
! TAAAACAAATTATTGGACGA+TGG + chr1_4:43814351-43814370 Msa0164400:intron 25.0%
!! CAGATAAATTTTATGTGATC+AGG - chr1_4:43814621-43814640 None:intergenic 25.0%
! CTGATCACATAAAATTTATC+TGG + chr1_4:43814619-43814638 Msa0164400:intron 25.0%
! TTTAAATCAAAGAATCGACA+AGG - chr1_4:43814748-43814767 None:intergenic 25.0%
! GCAATTATATACCAAATGAA+GGG + chr1_4:43814825-43814844 Msa0164400:CDS 25.0%
! TAAATAGTTCTTTCTCTTTG+TGG + chr1_4:43813343-43813362 Msa0164400:five_prime_UTR 25.0%
! CTTCATGATAATTTGAAGAA+AGG - chr1_4:43813424-43813443 None:intergenic 25.0%
! GTATGAGTTAAATATCAAGA+TGG + chr1_4:43813478-43813497 Msa0164400:CDS 25.0%
! TCTTTCAATCTATCCAATAT+GGG - chr1_4:43813653-43813672 None:intergenic 25.0%
!!! TTAGTTTGTTTTAATGTTGC+AGG - chr1_4:43813701-43813720 None:intergenic 25.0%
!!! ACAAACTAAGAAAGTTTTGA+AGG + chr1_4:43813712-43813731 Msa0164400:CDS 25.0%
! AACAATTATCTAACGTACTT+AGG - chr1_4:43813829-43813848 None:intergenic 25.0%
!!! ATCGTCCAATAATTTGTTTT+AGG - chr1_4:43814352-43814371 None:intergenic 25.0%
! TAAAACAAATTATTGGACGA+TGG + chr1_4:43814351-43814370 Msa0164400:intron 25.0%
!! CAGATAAATTTTATGTGATC+AGG - chr1_4:43814621-43814640 None:intergenic 25.0%
! CTGATCACATAAAATTTATC+TGG + chr1_4:43814619-43814638 Msa0164400:intron 25.0%
! TTTAAATCAAAGAATCGACA+AGG - chr1_4:43814748-43814767 None:intergenic 25.0%
! GCAATTATATACCAAATGAA+GGG + chr1_4:43814825-43814844 Msa0164400:CDS 25.0%
! AATTGATCAACTTTTCTACC+AGG - chr1_4:43813397-43813416 None:intergenic 30.0%
ATCGTTAGAGAAATTCATAG+TGG + chr1_4:43813623-43813642 Msa0164400:CDS 30.0%
CTCTTTCAATCTATCCAATA+TGG - chr1_4:43813654-43813673 None:intergenic 30.0%
CATATTGGATAGATTGAAAG+AGG + chr1_4:43813652-43813671 Msa0164400:CDS 30.0%
AGTAGAAATGCTCATCTATA+AGG + chr1_4:43814029-43814048 Msa0164400:CDS 30.0%
!!! ACATTTTTGGCCTCTAATTT+AGG + chr1_4:43814117-43814136 Msa0164400:intron 30.0%
ACAATTGAGAAACTACAATC+AGG - chr1_4:43814174-43814193 None:intergenic 30.0%
!! TAAAGAAAAGTTGATGGGAA+GGG - chr1_4:43814224-43814243 None:intergenic 30.0%
! CTCTCTAAAGAAAAGTTGAT+GGG - chr1_4:43814229-43814248 None:intergenic 30.0%
AAAAGAAAACTTCAATTGGC+AGG + chr1_4:43814429-43814448 Msa0164400:intron 30.0%
AAAGAAAACTTCAATTGGCA+GGG + chr1_4:43814430-43814449 Msa0164400:intron 30.0%
AATCATCTTTCAAGCATGTA+CGG - chr1_4:43814477-43814496 None:intergenic 30.0%
! GTATGCAAGTTGAAATGATA+AGG - chr1_4:43814541-43814560 None:intergenic 30.0%
TTATGATCTGATAAAGCACA+AGG - chr1_4:43814596-43814615 None:intergenic 30.0%
TCACGATCAACTTATTTCAA+AGG + chr1_4:43814668-43814687 Msa0164400:intron 30.0%
GGCAATTATATACCAAATGA+AGG + chr1_4:43814824-43814843 Msa0164400:intron 30.0%
GAATTCATTCCAACATTCAT+TGG - chr1_4:43814865-43814884 None:intergenic 30.0%
AGTGCAATACAAGAATTATC+AGG + chr1_4:43814957-43814976 Msa0164400:CDS 30.0%
GTGCAATACAAGAATTATCA+GGG + chr1_4:43814958-43814977 Msa0164400:CDS 30.0%
TGACTATAATGTTTACTGTG+AGG + chr1_4:43815072-43815091 Msa0164400:three_prime_UTR 30.0%
CAAACTCGTACAAATATTAG+AGG - chr1_4:43815261-43815280 None:intergenic 30.0%
! AATTGATCAACTTTTCTACC+AGG - chr1_4:43813397-43813416 None:intergenic 30.0%
ATCGTTAGAGAAATTCATAG+TGG + chr1_4:43813623-43813642 Msa0164400:CDS 30.0%
CTCTTTCAATCTATCCAATA+TGG - chr1_4:43813654-43813673 None:intergenic 30.0%
CATATTGGATAGATTGAAAG+AGG + chr1_4:43813652-43813671 Msa0164400:CDS 30.0%
AGTAGAAATGCTCATCTATA+AGG + chr1_4:43814029-43814048 Msa0164400:CDS 30.0%
!!! ACATTTTTGGCCTCTAATTT+AGG + chr1_4:43814117-43814136 Msa0164400:intron 30.0%
ACAATTGAGAAACTACAATC+AGG - chr1_4:43814174-43814193 None:intergenic 30.0%
!! TAAAGAAAAGTTGATGGGAA+GGG - chr1_4:43814224-43814243 None:intergenic 30.0%
! CTCTCTAAAGAAAAGTTGAT+GGG - chr1_4:43814229-43814248 None:intergenic 30.0%
AAAAGAAAACTTCAATTGGC+AGG + chr1_4:43814429-43814448 Msa0164400:intron 30.0%
AAAGAAAACTTCAATTGGCA+GGG + chr1_4:43814430-43814449 Msa0164400:intron 30.0%
AATCATCTTTCAAGCATGTA+CGG - chr1_4:43814477-43814496 None:intergenic 30.0%
! GTATGCAAGTTGAAATGATA+AGG - chr1_4:43814541-43814560 None:intergenic 30.0%
TTATGATCTGATAAAGCACA+AGG - chr1_4:43814596-43814615 None:intergenic 30.0%
TCACGATCAACTTATTTCAA+AGG + chr1_4:43814668-43814687 Msa0164400:intron 30.0%
GGCAATTATATACCAAATGA+AGG + chr1_4:43814824-43814843 Msa0164400:intron 30.0%
GAATTCATTCCAACATTCAT+TGG - chr1_4:43814865-43814884 None:intergenic 30.0%
AGTGCAATACAAGAATTATC+AGG + chr1_4:43814957-43814976 Msa0164400:CDS 30.0%
GTGCAATACAAGAATTATCA+GGG + chr1_4:43814958-43814977 Msa0164400:CDS 30.0%
TGACTATAATGTTTACTGTG+AGG + chr1_4:43815072-43815091 Msa0164400:three_prime_UTR 30.0%
CAAACTCGTACAAATATTAG+AGG - chr1_4:43815261-43815280 None:intergenic 30.0%
!! TGTGTGCTAAATGATTTGGT+AGG + chr1_4:43813310-43813329 Msa0164400:five_prime_UTR 35.0%
AAATATCAAGATGGCAACAC+TGG + chr1_4:43813487-43813506 Msa0164400:CDS 35.0%
AGTCTCTATCTTTCGACTTA+TGG + chr1_4:43813533-43813552 Msa0164400:CDS 35.0%
GTCTCTATCTTTCGACTTAT+GGG + chr1_4:43813534-43813553 Msa0164400:CDS 35.0%
! TCATAGTGGAAAACCCATAT+TGG + chr1_4:43813637-43813656 Msa0164400:CDS 35.0%
!! CTAAAGAAAAGTTGATGGGA+AGG - chr1_4:43814225-43814244 None:intergenic 35.0%
! CCTCTCTAAAGAAAAGTTGA+TGG - chr1_4:43814230-43814249 None:intergenic 35.0%
!! CCATCAACTTTTCTTTAGAG+AGG + chr1_4:43814227-43814246 Msa0164400:intron 35.0%
!! ATGGTGTTGCAAATGACATA+GGG + chr1_4:43814370-43814389 Msa0164400:intron 35.0%
GAAGATGTGCATTCGATTAT+TGG - chr1_4:43814726-43814745 None:intergenic 35.0%
ATACCAAATGAAGGGAAACA+CGG + chr1_4:43814833-43814852 Msa0164400:CDS 35.0%
CGGAAAAAACCAATGAATGT+TGG + chr1_4:43814853-43814872 Msa0164400:CDS 35.0%
ATGTTGGAATGAATTCCTTG+CGG + chr1_4:43814869-43814888 Msa0164400:CDS 35.0%
!! TAAAGAGAAAATTCGGTGCA+AGG - chr1_4:43814906-43814925 None:intergenic 35.0%
CAATACAAGAATTATCAGGG+AGG + chr1_4:43814961-43814980 Msa0164400:CDS 35.0%
!! TGTGTGCTAAATGATTTGGT+AGG + chr1_4:43813310-43813329 Msa0164400:five_prime_UTR 35.0%
AAATATCAAGATGGCAACAC+TGG + chr1_4:43813487-43813506 Msa0164400:CDS 35.0%
AGTCTCTATCTTTCGACTTA+TGG + chr1_4:43813533-43813552 Msa0164400:CDS 35.0%
GTCTCTATCTTTCGACTTAT+GGG + chr1_4:43813534-43813553 Msa0164400:CDS 35.0%
! TCATAGTGGAAAACCCATAT+TGG + chr1_4:43813637-43813656 Msa0164400:CDS 35.0%
!! CTAAAGAAAAGTTGATGGGA+AGG - chr1_4:43814225-43814244 None:intergenic 35.0%
! CCTCTCTAAAGAAAAGTTGA+TGG - chr1_4:43814230-43814249 None:intergenic 35.0%
!! CCATCAACTTTTCTTTAGAG+AGG + chr1_4:43814227-43814246 Msa0164400:intron 35.0%
!! ATGGTGTTGCAAATGACATA+GGG + chr1_4:43814370-43814389 Msa0164400:intron 35.0%
GAAGATGTGCATTCGATTAT+TGG - chr1_4:43814726-43814745 None:intergenic 35.0%
ATACCAAATGAAGGGAAACA+CGG + chr1_4:43814833-43814852 Msa0164400:CDS 35.0%
CGGAAAAAACCAATGAATGT+TGG + chr1_4:43814853-43814872 Msa0164400:CDS 35.0%
ATGTTGGAATGAATTCCTTG+CGG + chr1_4:43814869-43814888 Msa0164400:CDS 35.0%
!! TAAAGAGAAAATTCGGTGCA+AGG - chr1_4:43814906-43814925 None:intergenic 35.0%
CAATACAAGAATTATCAGGG+AGG + chr1_4:43814961-43814980 Msa0164400:CDS 35.0%
CCCAAGGATGACAATAACAA+CGG - chr1_4:43813513-43813532 None:intergenic 40.0%
! CCGTTGTTATTGTCATCCTT+GGG + chr1_4:43813510-43813529 Msa0164400:CDS 40.0%
TAACGATCCATGACCTTGTA+CGG - chr1_4:43813610-43813629 None:intergenic 40.0%
AGATATGTTGCAGCAAGCTT+TGG + chr1_4:43813891-43813910 Msa0164400:intron 40.0%
TTGGAAAAGCTGGAAATGAG+TGG + chr1_4:43813910-43813929 Msa0164400:CDS 40.0%
ACTGGAAAATGCCGTAAAGA+AGG + chr1_4:43813957-43813976 Msa0164400:CDS 40.0%
! CGCATTTTCAGCCTTCTTTA+CGG - chr1_4:43813971-43813990 None:intergenic 40.0%
!! GATGGTGTTGCAAATGACAT+AGG + chr1_4:43814369-43814388 Msa0164400:intron 40.0%
!! TGGTGTTGCAAATGACATAG+GGG + chr1_4:43814371-43814390 Msa0164400:intron 40.0%
TTTCAAGCATGTACGGTTCA+AGG - chr1_4:43814470-43814489 None:intergenic 40.0%
TTTCCGTGTTTCCCTTCATT+TGG - chr1_4:43814839-43814858 None:intergenic 40.0%
ACAAGAATTATCAGGGAGGA+AGG + chr1_4:43814965-43814984 Msa0164400:CDS 40.0%
CAAGAATTATCAGGGAGGAA+GGG + chr1_4:43814966-43814985 Msa0164400:CDS 40.0%
!!! GAGCCAGGAATTTTAACTAG+TGG + chr1_4:43815292-43815311 Msa0164400:three_prime_UTR 40.0%
CCTCCACTAGTTAAAATTCC+TGG - chr1_4:43815298-43815317 None:intergenic 40.0%
!!! CCAGGAATTTTAACTAGTGG+AGG + chr1_4:43815295-43815314 Msa0164400:three_prime_UTR 40.0%
CCCAAGGATGACAATAACAA+CGG - chr1_4:43813513-43813532 None:intergenic 40.0%
! CCGTTGTTATTGTCATCCTT+GGG + chr1_4:43813510-43813529 Msa0164400:CDS 40.0%
TAACGATCCATGACCTTGTA+CGG - chr1_4:43813610-43813629 None:intergenic 40.0%
AGATATGTTGCAGCAAGCTT+TGG + chr1_4:43813891-43813910 Msa0164400:intron 40.0%
TTGGAAAAGCTGGAAATGAG+TGG + chr1_4:43813910-43813929 Msa0164400:CDS 40.0%
ACTGGAAAATGCCGTAAAGA+AGG + chr1_4:43813957-43813976 Msa0164400:CDS 40.0%
! CGCATTTTCAGCCTTCTTTA+CGG - chr1_4:43813971-43813990 None:intergenic 40.0%
!! GATGGTGTTGCAAATGACAT+AGG + chr1_4:43814369-43814388 Msa0164400:intron 40.0%
!! TGGTGTTGCAAATGACATAG+GGG + chr1_4:43814371-43814390 Msa0164400:intron 40.0%
TTTCAAGCATGTACGGTTCA+AGG - chr1_4:43814470-43814489 None:intergenic 40.0%
TTTCCGTGTTTCCCTTCATT+TGG - chr1_4:43814839-43814858 None:intergenic 40.0%
ACAAGAATTATCAGGGAGGA+AGG + chr1_4:43814965-43814984 Msa0164400:CDS 40.0%
CAAGAATTATCAGGGAGGAA+GGG + chr1_4:43814966-43814985 Msa0164400:CDS 40.0%
!!! GAGCCAGGAATTTTAACTAG+TGG + chr1_4:43815292-43815311 Msa0164400:three_prime_UTR 40.0%
CCTCCACTAGTTAAAATTCC+TGG - chr1_4:43815298-43815317 None:intergenic 40.0%
!!! CCAGGAATTTTAACTAGTGG+AGG + chr1_4:43815295-43815314 Msa0164400:three_prime_UTR 40.0%
CGACTCAAGTATGAATCACC+TGG + chr1_4:43813376-43813395 Msa0164400:exon 45.0%
! GCCGTTGTTATTGTCATCCT+TGG + chr1_4:43813509-43813528 Msa0164400:CDS 45.0%
TCGAAAGATAGAGACTCCCA+AGG - chr1_4:43813529-43813548 None:intergenic 45.0%
TGAGCTTGAGCTTCGACTTT+CGG - chr1_4:43813565-43813584 None:intergenic 45.0%
ACTTTGACCGTACAAGGTCA+TGG + chr1_4:43813600-43813619 Msa0164400:CDS 45.0%
GAGTGGAAGTGAAGATTCTG+CGG + chr1_4:43813927-43813946 Msa0164400:CDS 45.0%
AGATTCTGCGGTGGAAATAC+TGG + chr1_4:43813939-43813958 Msa0164400:CDS 45.0%
!! AGTTGATGGGAAGGGTTGAA+GGG - chr1_4:43814216-43814235 None:intergenic 45.0%
!! AAGTTGATGGGAAGGGTTGA+AGG - chr1_4:43814217-43814236 None:intergenic 45.0%
GAACGTGTACCTTGTACAGT+GGG - chr1_4:43814519-43814538 None:intergenic 45.0%
!! AGAGAAAATTCGGTGCAAGG+AGG - chr1_4:43814903-43814922 None:intergenic 45.0%
GTACGAGTTTGACACAGTAG+CGG + chr1_4:43815270-43815289 Msa0164400:three_prime_UTR 45.0%
CGACTCAAGTATGAATCACC+TGG + chr1_4:43813376-43813395 Msa0164400:exon 45.0%
! GCCGTTGTTATTGTCATCCT+TGG + chr1_4:43813509-43813528 Msa0164400:CDS 45.0%
TCGAAAGATAGAGACTCCCA+AGG - chr1_4:43813529-43813548 None:intergenic 45.0%
TGAGCTTGAGCTTCGACTTT+CGG - chr1_4:43813565-43813584 None:intergenic 45.0%
ACTTTGACCGTACAAGGTCA+TGG + chr1_4:43813600-43813619 Msa0164400:CDS 45.0%
GAGTGGAAGTGAAGATTCTG+CGG + chr1_4:43813927-43813946 Msa0164400:CDS 45.0%
AGATTCTGCGGTGGAAATAC+TGG + chr1_4:43813939-43813958 Msa0164400:CDS 45.0%
!! AGTTGATGGGAAGGGTTGAA+GGG - chr1_4:43814216-43814235 None:intergenic 45.0%
!! AAGTTGATGGGAAGGGTTGA+AGG - chr1_4:43814217-43814236 None:intergenic 45.0%
GAACGTGTACCTTGTACAGT+GGG - chr1_4:43814519-43814538 None:intergenic 45.0%
!! AGAGAAAATTCGGTGCAAGG+AGG - chr1_4:43814903-43814922 None:intergenic 45.0%
GTACGAGTTTGACACAGTAG+CGG + chr1_4:43815270-43815289 Msa0164400:three_prime_UTR 45.0%
! CCTTGTACGGTCAAAGTGTC+CGG - chr1_4:43813597-43813616 None:intergenic 50.0%
!! GCAGCAAGCTTTGGAAAAGC+TGG + chr1_4:43813900-43813919 Msa0164400:intron 50.0%
TGGAAGTGAAGATTCTGCGG+TGG + chr1_4:43813930-43813949 Msa0164400:CDS 50.0%
GGAACGTGTACCTTGTACAG+TGG - chr1_4:43814520-43814539 None:intergenic 50.0%
AAGGAGGATCTTGTACCGCA+AGG - chr1_4:43814887-43814906 None:intergenic 50.0%
! CCTTGTACGGTCAAAGTGTC+CGG - chr1_4:43813597-43813616 None:intergenic 50.0%
!! GCAGCAAGCTTTGGAAAAGC+TGG + chr1_4:43813900-43813919 Msa0164400:intron 50.0%
TGGAAGTGAAGATTCTGCGG+TGG + chr1_4:43813930-43813949 Msa0164400:CDS 50.0%
GGAACGTGTACCTTGTACAG+TGG - chr1_4:43814520-43814539 None:intergenic 50.0%
AAGGAGGATCTTGTACCGCA+AGG - chr1_4:43814887-43814906 None:intergenic 50.0%
! CGGTCAAAGTGTCCGGACTT+GGG - chr1_4:43813590-43813609 None:intergenic 55.0%
! ACGGTCAAAGTGTCCGGACT+TGG - chr1_4:43813591-43813610 None:intergenic 55.0%
CCGGACACTTTGACCGTACA+AGG + chr1_4:43813594-43813613 Msa0164400:CDS 55.0%
CTTCAATTGGCAGGGAGAGC+TGG + chr1_4:43814438-43814457 Msa0164400:intron 55.0%
! TTTGACACAGTAGCGGAGCC+AGG + chr1_4:43815277-43815296 Msa0164400:three_prime_UTR 55.0%
! CGGTCAAAGTGTCCGGACTT+GGG - chr1_4:43813590-43813609 None:intergenic 55.0%
! ACGGTCAAAGTGTCCGGACT+TGG - chr1_4:43813591-43813610 None:intergenic 55.0%
CCGGACACTTTGACCGTACA+AGG + chr1_4:43813594-43813613 Msa0164400:CDS 55.0%
CTTCAATTGGCAGGGAGAGC+TGG + chr1_4:43814438-43814457 Msa0164400:intron 55.0%
! TTTGACACAGTAGCGGAGCC+AGG + chr1_4:43815277-43815296 Msa0164400:three_prime_UTR 55.0%
CTCAAGCTCACGCCCAAGTC+CGG + chr1_4:43813575-43813594 Msa0164400:CDS 60.0%
TGCTCGACGCCCACTGTACA+AGG + chr1_4:43814507-43814526 Msa0164400:CDS 60.0%
CTCAAGCTCACGCCCAAGTC+CGG + chr1_4:43813575-43813594 Msa0164400:CDS 60.0%
TGCTCGACGCCCACTGTACA+AGG + chr1_4:43814507-43814526 Msa0164400:CDS 60.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 43813309 43815366 43813309 ID=Msa0164400;Name=Msa0164400
chr1_4 mRNA 43813309 43815366 43813309 ID=Msa0164400-mRNA-1;Parent=Msa0164400;Name=Msa0164400-mRNA-1;_AED=0.01;_eAED=0.01;_QI=77|1|1|1|1|1|4|371|261
chr1_4 exon 43813309 43813775 43813309 ID=Msa0164400-mRNA-1:exon:12529;Parent=Msa0164400-mRNA-1
chr1_4 exon 43813904 43814050 43813904 ID=Msa0164400-mRNA-1:exon:12530;Parent=Msa0164400-mRNA-1
chr1_4 exon 43814451 43814528 43814451 ID=Msa0164400-mRNA-1:exon:12531;Parent=Msa0164400-mRNA-1
chr1_4 exon 43814825 43815366 43814825 ID=Msa0164400-mRNA-1:exon:12532;Parent=Msa0164400-mRNA-1
chr1_4 five_prime_UTR 43813309 43813385 43813309 ID=Msa0164400-mRNA-1:five_prime_utr;Parent=Msa0164400-mRNA-1
chr1_4 CDS 43813386 43813775 43813386 ID=Msa0164400-mRNA-1:cds;Parent=Msa0164400-mRNA-1
chr1_4 CDS 43813904 43814050 43813904 ID=Msa0164400-mRNA-1:cds;Parent=Msa0164400-mRNA-1
chr1_4 CDS 43814451 43814528 43814451 ID=Msa0164400-mRNA-1:cds;Parent=Msa0164400-mRNA-1
chr1_4 CDS 43814825 43814995 43814825 ID=Msa0164400-mRNA-1:cds;Parent=Msa0164400-mRNA-1
chr1_4 three_prime_UTR 43814996 43815366 43814996 ID=Msa0164400-mRNA-1:three_prime_utr;Parent=Msa0164400-mRNA-1
Gene Sequence

>Msa0164400

ATGAATCACCTGGTAGAAAAGTTGATCAATTTATCCCTTTCTTCAAATTATCATGAAGATATGCGAAAATTCATCTTAGCTTTCACATCTAAGTATGAGTTAAATATCAAGATGGCAACACTGGCCGTTGTTATTGTCATCCTTGGGAGTCTCTATCTTTCGACTTATGGGTCAAACCGAAAGTCGAAGCTCAAGCTCACGCCCAAGTCCGGACACTTTGACCGTACAAGGTCATGGATCGTTAGAGAAATTCATAGTGGAAAACCCATATTGGATAGATTGAAAGAGGATTTCAATAAAGCTCGAGTAAATCCTGCAACATTAAAACAAACTAAGAAAGTTTTGAAGGCTTTACTCAAAGAAGAATATCTTGATTTAATAAAGATTCAGCAAGCTTTGGAAAAGCTGGAAATGAGTGGAAGTGAAGATTCTGCGGTGGAAATACTGGAAAATGCCGTAAAGAAGGCTGAAAATGCGAACAAAGCACATGAAGCTTATGAAATTGAAATGTTACTAGTAGAAATGCTCATCTATAAGGGAGAGCTGGACAGTGCCTTGAACCGTACATGCTTGAAAGATGATTCGCTTAAAGATGCTCGACGCCCACTGTACAAGGCAATTATATACCAAATGAAGGGAAACACGGAAAAAACCAATGAATGTTGGAATGAATTCCTTGCGGTACAAGATCCTCCTTGCACCGAATTTTCTCTTTATAAGTTCAAAAAAAATGTGGAACGACTTCAAAGTGCAATACAAGAATTATCAGGGAGGAAGGGACAATAA
Protein sequence

>Msa0164400

MNHLVEKLINLSLSSNYHEDMRKFILAFTSKYELNIKMATLAVVIVILGSLYLSTYGSNRKSKLKLTPKSGHFDRTRSWIVREIHSGKPILDRLKEDFNKARVNPATLKQTKKVLKALLKEEYLDLIKIQQALEKLEMSGSEDSAVEILENAVKKAENANKAHEAYEIEMLLVEMLIYKGELDSALNRTCLKDDSLKDARRPLYKAIIYQMKGNTEKTNECWNEFLAVQDPPCTEFSLYKFKKNVERLQSAIQELSGRKGQ*