AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0194840

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0194840 MtrunA17_Chr2g0285961 90.421 261 25 0 1 261 1 261 2.00e-175 483
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0194840 AT2G34530.1 38.424 203 111 5 70 259 67 268 1.98e-30 115
Msa0194840 AT2G34530.4 38.424 203 111 5 70 259 77 278 2.43e-30 115
Msa0194840 AT2G34530.3 38.424 203 111 5 70 259 111 312 4.28e-30 115
Msa0194840 AT2G34530.5 43.885 139 75 2 70 206 77 214 2.18e-25 100
Msa0194840 AT2G34530.2 43.885 139 75 2 70 206 67 204 4.85e-25 100

Find 64 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTCTCTATCTTTCGACTTA+TGG 0.180793 2_1:+11254385 Msa0194840:CDS
CTCTTTCAATCTATCCAATA+TGG 0.186585 2_1:-11254503 None:intergenic
TCTTGATTTAATAAAGATTC+AGG 0.264321 2_1:+11254606 Msa0194840:CDS
GTCTCTATCTTTCGACTTAT+GGG 0.294022 2_1:+11254386 Msa0194840:CDS
TAAATAGTTCTTTCTCTTTG+TGG 0.306506 2_1:+11254195 Msa0194840:five_prime_UTR
AGTGCAATACAAGAATTATC+AGG 0.313725 2_1:+11255809 Msa0194840:CDS
GCCGTTGTTATTGTCATCCT+TGG 0.313936 2_1:+11254361 Msa0194840:CDS
AGTAGAAATGCTCATCTATA+AGG 0.325228 2_1:+11254881 Msa0194840:CDS
CACTTGTGTGCTAAATGATT+TGG 0.330580 2_1:+11254158 None:intergenic
TGAGCTTGAGCTTCGACTTT+CGG 0.331242 2_1:-11254414 None:intergenic
ATCATTATATGTTAGATAAT+AGG 0.341955 2_1:+11255862 Msa0194840:three_prime_UTR
GAATTCATTCCAACATTCAT+TGG 0.362634 2_1:-11255714 None:intergenic
GAACTTATAAAGAGAAAATT+CGG 0.363931 2_1:-11255762 None:intergenic
TCATAGTGGAAAACCCATAT+TGG 0.366180 2_1:+11254489 Msa0194840:CDS
TTTCCGTGTTTCCCTTCATT+TGG 0.370913 2_1:-11255688 None:intergenic
AGATATGTTGCAGCAAGCTT+TGG 0.373596 2_1:+11254743 Msa0194840:intron
TTCATGATAATTTGAAGAAA+GGG 0.388116 2_1:-11254272 None:intergenic
CCTCCACTAGTTAAAATTCC+TGG 0.388708 2_1:-11256147 None:intergenic
ACGGTCAAAGTGTCCGGACT+TGG 0.391154 2_1:-11254440 None:intergenic
TCTTTCAATCTATCCAATAT+GGG 0.393501 2_1:-11254502 None:intergenic
CTTCATGATAATTTGAAGAA+AGG 0.402053 2_1:-11254273 None:intergenic
AGATTCTGCGGTGGAAATAC+TGG 0.405408 2_1:+11254791 Msa0194840:CDS
CTTCAATTGGCAGGGAGAGC+TGG 0.406149 2_1:+11255290 Msa0194840:intron
CTCAAGCTCACGCCCAAGTC+CGG 0.417252 2_1:+11254427 Msa0194840:CDS
CAAACTCGTACAAATATTAG+AGG 0.428879 2_1:-11256110 None:intergenic
CCGTTGTTATTGTCATCCTT+GGG 0.433089 2_1:+11254362 Msa0194840:CDS
GGCAATTATATACCAAATGA+AGG 0.439320 2_1:+11255676 Msa0194840:intron
TTTCAAGCATGTACGGTTCA+AGG 0.445106 2_1:-11255319 None:intergenic
AGTAATTTATAATTGAAGTC+AGG 0.472849 2_1:+11256019 Msa0194840:three_prime_UTR
CATATTGGATAGATTGAAAG+AGG 0.477246 2_1:+11254504 Msa0194840:CDS
CCGGACACTTTGACCGTACA+AGG 0.481049 2_1:+11254446 Msa0194840:CDS
TGCTCGACGCCCACTGTACA+AGG 0.484519 2_1:+11255359 Msa0194840:CDS
GCAGCAAGCTTTGGAAAAGC+TGG 0.488777 2_1:+11254752 Msa0194840:intron
ACTGGAAAATGCCGTAAAGA+AGG 0.511553 2_1:+11254809 Msa0194840:CDS
TAACGATCCATGACCTTGTA+CGG 0.512166 2_1:-11254459 None:intergenic
ACAAGAATTATCAGGGAGGA+AGG 0.520561 2_1:+11255817 Msa0194840:CDS
CCTTGTACGGTCAAAGTGTC+CGG 0.522757 2_1:-11254446 None:intergenic
CGGTCAAAGTGTCCGGACTT+GGG 0.540111 2_1:-11254439 None:intergenic
TTTGACACACTAGCGGAGCC+AGG 0.551694 2_1:+11256129 Msa0194840:three_prime_UTR
AATCATCTTTCAAGCATGTA+CGG 0.563613 2_1:-11255326 None:intergenic
TAAAGAGAAAATTCGGTGCA+AGG 0.565220 2_1:-11255755 None:intergenic
ACTTTGACCGTACAAGGTCA+TGG 0.569622 2_1:+11254452 Msa0194840:CDS
GTGCAATACAAGAATTATCA+GGG 0.569824 2_1:+11255810 Msa0194840:CDS
AAATATCAAGATGGCAACAC+TGG 0.574878 2_1:+11254339 Msa0194840:CDS
CGACTCAAGTATGAATCACC+TGG 0.592794 2_1:+11254228 Msa0194840:exon
GCAATTATATACCAAATGAA+GGG 0.605208 2_1:+11255677 Msa0194840:CDS
CAAGAATTATCAGGGAGGAA+GGG 0.609044 2_1:+11255818 Msa0194840:CDS
TTGGAAAAGCTGGAAATGAG+TGG 0.622925 2_1:+11254762 Msa0194840:CDS
TGACTATAATGTTTACTGTG+AGG 0.625488 2_1:+11255924 Msa0194840:three_prime_UTR
GTATGAGTTAAATATCAAGA+TGG 0.627283 2_1:+11254330 Msa0194840:CDS
GAACGTGTACCTTGTACAGT+GGG 0.633465 2_1:-11255368 None:intergenic
GGAACGTGTACCTTGTACAG+TGG 0.634635 2_1:-11255369 None:intergenic
ATACCAAATGAAGGGAAACA+CGG 0.638175 2_1:+11255685 Msa0194840:CDS
AAGGAGGATCTTGTACCGCA+AGG 0.649015 2_1:-11255736 None:intergenic
ATCGTTAGAGAAATTCATAG+TGG 0.664835 2_1:+11254475 Msa0194840:CDS
TCGAAAGATAGAGACTCCCA+AGG 0.672047 2_1:-11254378 None:intergenic
GTACGAGTTTGACACACTAG+CGG 0.674704 2_1:+11256122 Msa0194840:three_prime_UTR
CCCAAGGATGACAATAACAA+CGG 0.691024 2_1:-11254362 None:intergenic
TGGAAGTGAAGATTCTGCGG+TGG 0.692864 2_1:+11254782 Msa0194840:CDS
AGAGAAAATTCGGTGCAAGG+AGG 0.706718 2_1:-11255752 None:intergenic
GAGTGGAAGTGAAGATTCTG+CGG 0.718050 2_1:+11254779 Msa0194840:CDS
TGTGTGCTAAATGATTTGGT+AGG 0.727793 2_1:+11254162 Msa0194840:five_prime_UTR
ATGTTGGAATGAATTCCTTG+CGG 0.756613 2_1:+11255721 Msa0194840:CDS
CAATACAAGAATTATCAGGG+AGG 0.773135 2_1:+11255813 Msa0194840:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAAGTTTATTTACATTTT+TGG + chr2_1:11254956-11254975 Msa0194840:intron 10.0%
!!! TTTAAGTTTATTTACATTTT+TGG + chr2_1:11254956-11254975 Msa0194840:intron 10.0%
!!! TAATTTAGGTATATATGTTT+TGG + chr2_1:11254983-11255002 Msa0194840:intron 15.0%
!!! AATGAACTTTTATAATCTAA+TGG + chr2_1:11255125-11255144 Msa0194840:intron 15.0%
!! TTATAAGTTCAAAAAAAATG+TGG + chr2_1:11255775-11255794 Msa0194840:CDS 15.0%
!! ATCATTATATGTTAGATAAT+AGG + chr2_1:11255862-11255881 Msa0194840:three_prime_UTR 15.0%
!!! TAATTTAGGTATATATGTTT+TGG + chr2_1:11254983-11255002 Msa0194840:intron 15.0%
!!! AATGAACTTTTATAATCTAA+TGG + chr2_1:11255125-11255144 Msa0194840:intron 15.0%
!! TTATAAGTTCAAAAAAAATG+TGG + chr2_1:11255775-11255794 Msa0194840:CDS 15.0%
!! ATCATTATATGTTAGATAAT+AGG + chr2_1:11255862-11255881 Msa0194840:three_prime_UTR 15.0%
!! TTCATGATAATTTGAAGAAA+GGG - chr2_1:11254275-11254294 None:intergenic 20.0%
!! TCTTGATTTAATAAAGATTC+AGG + chr2_1:11254606-11254625 Msa0194840:CDS 20.0%
!! AACAATAAATCTTAGTATGA+AGG - chr2_1:11254650-11254669 None:intergenic 20.0%
!! AACATATATACCTAAATTAG+AGG - chr2_1:11254982-11255001 None:intergenic 20.0%
!! ACGAAATTAATGTTAAATCT+TGG + chr2_1:11255154-11255173 Msa0194840:intron 20.0%
!!! CATTGAAATTTCAAGTTATT+AGG - chr2_1:11255180-11255199 None:intergenic 20.0%
!! AATGACCTAAAACAAATTAT+TGG + chr2_1:11255196-11255215 Msa0194840:intron 20.0%
!! TATGAAAAGAAAACTTCAAT+TGG + chr2_1:11255277-11255296 Msa0194840:intron 20.0%
!!! TATTTGATAATTTAGATGAG+AGG - chr2_1:11255415-11255434 None:intergenic 20.0%
!!! TTGATTCTTTCTTTTTTTAC+AGG + chr2_1:11255655-11255674 Msa0194840:intron 20.0%
!! GAACTTATAAAGAGAAAATT+CGG - chr2_1:11255765-11255784 None:intergenic 20.0%
!! AGTAATTTATAATTGAAGTC+AGG + chr2_1:11256019-11256038 Msa0194840:three_prime_UTR 20.0%
!! TTCATGATAATTTGAAGAAA+GGG - chr2_1:11254275-11254294 None:intergenic 20.0%
!! TCTTGATTTAATAAAGATTC+AGG + chr2_1:11254606-11254625 Msa0194840:CDS 20.0%
!! AACAATAAATCTTAGTATGA+AGG - chr2_1:11254650-11254669 None:intergenic 20.0%
!! AACATATATACCTAAATTAG+AGG - chr2_1:11254982-11255001 None:intergenic 20.0%
!! ACGAAATTAATGTTAAATCT+TGG + chr2_1:11255154-11255173 Msa0194840:intron 20.0%
!!! CATTGAAATTTCAAGTTATT+AGG - chr2_1:11255180-11255199 None:intergenic 20.0%
!! AATGACCTAAAACAAATTAT+TGG + chr2_1:11255196-11255215 Msa0194840:intron 20.0%
!! TATGAAAAGAAAACTTCAAT+TGG + chr2_1:11255277-11255296 Msa0194840:intron 20.0%
!!! TATTTGATAATTTAGATGAG+AGG - chr2_1:11255415-11255434 None:intergenic 20.0%
!!! TTGATTCTTTCTTTTTTTAC+AGG + chr2_1:11255655-11255674 Msa0194840:intron 20.0%
!! GAACTTATAAAGAGAAAATT+CGG - chr2_1:11255765-11255784 None:intergenic 20.0%
!! AGTAATTTATAATTGAAGTC+AGG + chr2_1:11256019-11256038 Msa0194840:three_prime_UTR 20.0%
! TAAATAGTTCTTTCTCTTTG+TGG + chr2_1:11254195-11254214 Msa0194840:five_prime_UTR 25.0%
! CTTCATGATAATTTGAAGAA+AGG - chr2_1:11254276-11254295 None:intergenic 25.0%
! GTATGAGTTAAATATCAAGA+TGG + chr2_1:11254330-11254349 Msa0194840:CDS 25.0%
! TCTTTCAATCTATCCAATAT+GGG - chr2_1:11254505-11254524 None:intergenic 25.0%
!!! TTAGTTTGTTTTAATGTTGC+AGG - chr2_1:11254553-11254572 None:intergenic 25.0%
!!! ACAAACTAAGAAAGTTTTGA+AGG + chr2_1:11254564-11254583 Msa0194840:CDS 25.0%
! AACAATTATCTAACGTACTT+AGG - chr2_1:11254681-11254700 None:intergenic 25.0%
!!! ATCGTCCAATAATTTGTTTT+AGG - chr2_1:11255204-11255223 None:intergenic 25.0%
! TAAAACAAATTATTGGACGA+TGG + chr2_1:11255203-11255222 Msa0194840:intron 25.0%
!! CAGATAAATTTTATGTGATC+AGG - chr2_1:11255473-11255492 None:intergenic 25.0%
! CTGATCACATAAAATTTATC+TGG + chr2_1:11255471-11255490 Msa0194840:intron 25.0%
!! TTTTAATCAAAGAATCGACA+AGG - chr2_1:11255600-11255619 None:intergenic 25.0%
! GCAATTATATACCAAATGAA+GGG + chr2_1:11255677-11255696 Msa0194840:CDS 25.0%
! TAAATAGTTCTTTCTCTTTG+TGG + chr2_1:11254195-11254214 Msa0194840:five_prime_UTR 25.0%
! CTTCATGATAATTTGAAGAA+AGG - chr2_1:11254276-11254295 None:intergenic 25.0%
! GTATGAGTTAAATATCAAGA+TGG + chr2_1:11254330-11254349 Msa0194840:CDS 25.0%
! TCTTTCAATCTATCCAATAT+GGG - chr2_1:11254505-11254524 None:intergenic 25.0%
!!! TTAGTTTGTTTTAATGTTGC+AGG - chr2_1:11254553-11254572 None:intergenic 25.0%
!!! ACAAACTAAGAAAGTTTTGA+AGG + chr2_1:11254564-11254583 Msa0194840:CDS 25.0%
! AACAATTATCTAACGTACTT+AGG - chr2_1:11254681-11254700 None:intergenic 25.0%
!!! ATCGTCCAATAATTTGTTTT+AGG - chr2_1:11255204-11255223 None:intergenic 25.0%
! TAAAACAAATTATTGGACGA+TGG + chr2_1:11255203-11255222 Msa0194840:intron 25.0%
!! CAGATAAATTTTATGTGATC+AGG - chr2_1:11255473-11255492 None:intergenic 25.0%
! CTGATCACATAAAATTTATC+TGG + chr2_1:11255471-11255490 Msa0194840:intron 25.0%
!! TTTTAATCAAAGAATCGACA+AGG - chr2_1:11255600-11255619 None:intergenic 25.0%
! GCAATTATATACCAAATGAA+GGG + chr2_1:11255677-11255696 Msa0194840:CDS 25.0%
! AATTGATCAACTTTTCTACC+AGG - chr2_1:11254249-11254268 None:intergenic 30.0%
ATCGTTAGAGAAATTCATAG+TGG + chr2_1:11254475-11254494 Msa0194840:CDS 30.0%
CTCTTTCAATCTATCCAATA+TGG - chr2_1:11254506-11254525 None:intergenic 30.0%
CATATTGGATAGATTGAAAG+AGG + chr2_1:11254504-11254523 Msa0194840:CDS 30.0%
AGTAGAAATGCTCATCTATA+AGG + chr2_1:11254881-11254900 Msa0194840:CDS 30.0%
!!! ACATTTTTGGCCTCTAATTT+AGG + chr2_1:11254969-11254988 Msa0194840:intron 30.0%
ACAATTGAGAAACTACAATC+AGG - chr2_1:11255026-11255045 None:intergenic 30.0%
!! TAAAGAAAAGTTGATGGGAA+GGG - chr2_1:11255076-11255095 None:intergenic 30.0%
! CTCTCTAAAGAAAAGTTGAT+GGG - chr2_1:11255081-11255100 None:intergenic 30.0%
AAAAGAAAACTTCAATTGGC+AGG + chr2_1:11255281-11255300 Msa0194840:intron 30.0%
AAAGAAAACTTCAATTGGCA+GGG + chr2_1:11255282-11255301 Msa0194840:intron 30.0%
AATCATCTTTCAAGCATGTA+CGG - chr2_1:11255329-11255348 None:intergenic 30.0%
! GTATGCAAGTTGAAATGATA+AGG - chr2_1:11255393-11255412 None:intergenic 30.0%
TCAAAAACCTTGTGCTTTAT+CGG + chr2_1:11255438-11255457 Msa0194840:intron 30.0%
TCACGATCAACTTATTTCAA+AGG + chr2_1:11255520-11255539 Msa0194840:intron 30.0%
GGCAATTATATACCAAATGA+AGG + chr2_1:11255676-11255695 Msa0194840:intron 30.0%
GAATTCATTCCAACATTCAT+TGG - chr2_1:11255717-11255736 None:intergenic 30.0%
AGTGCAATACAAGAATTATC+AGG + chr2_1:11255809-11255828 Msa0194840:CDS 30.0%
GTGCAATACAAGAATTATCA+GGG + chr2_1:11255810-11255829 Msa0194840:CDS 30.0%
TGACTATAATGTTTACTGTG+AGG + chr2_1:11255924-11255943 Msa0194840:three_prime_UTR 30.0%
CAAACTCGTACAAATATTAG+AGG - chr2_1:11256113-11256132 None:intergenic 30.0%
! AATTGATCAACTTTTCTACC+AGG - chr2_1:11254249-11254268 None:intergenic 30.0%
ATCGTTAGAGAAATTCATAG+TGG + chr2_1:11254475-11254494 Msa0194840:CDS 30.0%
CTCTTTCAATCTATCCAATA+TGG - chr2_1:11254506-11254525 None:intergenic 30.0%
CATATTGGATAGATTGAAAG+AGG + chr2_1:11254504-11254523 Msa0194840:CDS 30.0%
AGTAGAAATGCTCATCTATA+AGG + chr2_1:11254881-11254900 Msa0194840:CDS 30.0%
!!! ACATTTTTGGCCTCTAATTT+AGG + chr2_1:11254969-11254988 Msa0194840:intron 30.0%
ACAATTGAGAAACTACAATC+AGG - chr2_1:11255026-11255045 None:intergenic 30.0%
!! TAAAGAAAAGTTGATGGGAA+GGG - chr2_1:11255076-11255095 None:intergenic 30.0%
! CTCTCTAAAGAAAAGTTGAT+GGG - chr2_1:11255081-11255100 None:intergenic 30.0%
AAAAGAAAACTTCAATTGGC+AGG + chr2_1:11255281-11255300 Msa0194840:intron 30.0%
AAAGAAAACTTCAATTGGCA+GGG + chr2_1:11255282-11255301 Msa0194840:intron 30.0%
AATCATCTTTCAAGCATGTA+CGG - chr2_1:11255329-11255348 None:intergenic 30.0%
! GTATGCAAGTTGAAATGATA+AGG - chr2_1:11255393-11255412 None:intergenic 30.0%
TCAAAAACCTTGTGCTTTAT+CGG + chr2_1:11255438-11255457 Msa0194840:intron 30.0%
TCACGATCAACTTATTTCAA+AGG + chr2_1:11255520-11255539 Msa0194840:intron 30.0%
GGCAATTATATACCAAATGA+AGG + chr2_1:11255676-11255695 Msa0194840:intron 30.0%
GAATTCATTCCAACATTCAT+TGG - chr2_1:11255717-11255736 None:intergenic 30.0%
AGTGCAATACAAGAATTATC+AGG + chr2_1:11255809-11255828 Msa0194840:CDS 30.0%
GTGCAATACAAGAATTATCA+GGG + chr2_1:11255810-11255829 Msa0194840:CDS 30.0%
TGACTATAATGTTTACTGTG+AGG + chr2_1:11255924-11255943 Msa0194840:three_prime_UTR 30.0%
CAAACTCGTACAAATATTAG+AGG - chr2_1:11256113-11256132 None:intergenic 30.0%
!! TGTGTGCTAAATGATTTGGT+AGG + chr2_1:11254162-11254181 Msa0194840:five_prime_UTR 35.0%
AAATATCAAGATGGCAACAC+TGG + chr2_1:11254339-11254358 Msa0194840:CDS 35.0%
AGTCTCTATCTTTCGACTTA+TGG + chr2_1:11254385-11254404 Msa0194840:CDS 35.0%
GTCTCTATCTTTCGACTTAT+GGG + chr2_1:11254386-11254405 Msa0194840:CDS 35.0%
! TCATAGTGGAAAACCCATAT+TGG + chr2_1:11254489-11254508 Msa0194840:CDS 35.0%
!! CTAAAGAAAAGTTGATGGGA+AGG - chr2_1:11255077-11255096 None:intergenic 35.0%
! CCTCTCTAAAGAAAAGTTGA+TGG - chr2_1:11255082-11255101 None:intergenic 35.0%
!! CCATCAACTTTTCTTTAGAG+AGG + chr2_1:11255079-11255098 Msa0194840:intron 35.0%
!! ATGGTGTTGCAAATGACATA+GGG + chr2_1:11255222-11255241 Msa0194840:intron 35.0%
TTATGATCCGATAAAGCACA+AGG - chr2_1:11255448-11255467 None:intergenic 35.0%
GAAGATGTGCATTCGATTAT+TGG - chr2_1:11255578-11255597 None:intergenic 35.0%
ATACCAAATGAAGGGAAACA+CGG + chr2_1:11255685-11255704 Msa0194840:CDS 35.0%
CGGAAAAAACCAATGAATGT+TGG + chr2_1:11255705-11255724 Msa0194840:CDS 35.0%
ATGTTGGAATGAATTCCTTG+CGG + chr2_1:11255721-11255740 Msa0194840:CDS 35.0%
!! TAAAGAGAAAATTCGGTGCA+AGG - chr2_1:11255758-11255777 None:intergenic 35.0%
CAATACAAGAATTATCAGGG+AGG + chr2_1:11255813-11255832 Msa0194840:CDS 35.0%
!! TGTGTGCTAAATGATTTGGT+AGG + chr2_1:11254162-11254181 Msa0194840:five_prime_UTR 35.0%
AAATATCAAGATGGCAACAC+TGG + chr2_1:11254339-11254358 Msa0194840:CDS 35.0%
AGTCTCTATCTTTCGACTTA+TGG + chr2_1:11254385-11254404 Msa0194840:CDS 35.0%
GTCTCTATCTTTCGACTTAT+GGG + chr2_1:11254386-11254405 Msa0194840:CDS 35.0%
! TCATAGTGGAAAACCCATAT+TGG + chr2_1:11254489-11254508 Msa0194840:CDS 35.0%
!! CTAAAGAAAAGTTGATGGGA+AGG - chr2_1:11255077-11255096 None:intergenic 35.0%
! CCTCTCTAAAGAAAAGTTGA+TGG - chr2_1:11255082-11255101 None:intergenic 35.0%
!! CCATCAACTTTTCTTTAGAG+AGG + chr2_1:11255079-11255098 Msa0194840:intron 35.0%
!! ATGGTGTTGCAAATGACATA+GGG + chr2_1:11255222-11255241 Msa0194840:intron 35.0%
TTATGATCCGATAAAGCACA+AGG - chr2_1:11255448-11255467 None:intergenic 35.0%
GAAGATGTGCATTCGATTAT+TGG - chr2_1:11255578-11255597 None:intergenic 35.0%
ATACCAAATGAAGGGAAACA+CGG + chr2_1:11255685-11255704 Msa0194840:CDS 35.0%
CGGAAAAAACCAATGAATGT+TGG + chr2_1:11255705-11255724 Msa0194840:CDS 35.0%
ATGTTGGAATGAATTCCTTG+CGG + chr2_1:11255721-11255740 Msa0194840:CDS 35.0%
!! TAAAGAGAAAATTCGGTGCA+AGG - chr2_1:11255758-11255777 None:intergenic 35.0%
CAATACAAGAATTATCAGGG+AGG + chr2_1:11255813-11255832 Msa0194840:CDS 35.0%
CCCAAGGATGACAATAACAA+CGG - chr2_1:11254365-11254384 None:intergenic 40.0%
! CCGTTGTTATTGTCATCCTT+GGG + chr2_1:11254362-11254381 Msa0194840:CDS 40.0%
TAACGATCCATGACCTTGTA+CGG - chr2_1:11254462-11254481 None:intergenic 40.0%
AGATATGTTGCAGCAAGCTT+TGG + chr2_1:11254743-11254762 Msa0194840:intron 40.0%
TTGGAAAAGCTGGAAATGAG+TGG + chr2_1:11254762-11254781 Msa0194840:CDS 40.0%
ACTGGAAAATGCCGTAAAGA+AGG + chr2_1:11254809-11254828 Msa0194840:CDS 40.0%
! CGCATTTTCAGCCTTCTTTA+CGG - chr2_1:11254823-11254842 None:intergenic 40.0%
!! GATGGTGTTGCAAATGACAT+AGG + chr2_1:11255221-11255240 Msa0194840:intron 40.0%
!! TGGTGTTGCAAATGACATAG+GGG + chr2_1:11255223-11255242 Msa0194840:intron 40.0%
TTTCAAGCATGTACGGTTCA+AGG - chr2_1:11255322-11255341 None:intergenic 40.0%
TTTCCGTGTTTCCCTTCATT+TGG - chr2_1:11255691-11255710 None:intergenic 40.0%
ACAAGAATTATCAGGGAGGA+AGG + chr2_1:11255817-11255836 Msa0194840:CDS 40.0%
CAAGAATTATCAGGGAGGAA+GGG + chr2_1:11255818-11255837 Msa0194840:CDS 40.0%
!!! GAGCCAGGAATTTTAACTAG+TGG + chr2_1:11256144-11256163 Msa0194840:three_prime_UTR 40.0%
CCTCCACTAGTTAAAATTCC+TGG - chr2_1:11256150-11256169 None:intergenic 40.0%
!!! CCAGGAATTTTAACTAGTGG+AGG + chr2_1:11256147-11256166 Msa0194840:three_prime_UTR 40.0%
CCCAAGGATGACAATAACAA+CGG - chr2_1:11254365-11254384 None:intergenic 40.0%
! CCGTTGTTATTGTCATCCTT+GGG + chr2_1:11254362-11254381 Msa0194840:CDS 40.0%
TAACGATCCATGACCTTGTA+CGG - chr2_1:11254462-11254481 None:intergenic 40.0%
AGATATGTTGCAGCAAGCTT+TGG + chr2_1:11254743-11254762 Msa0194840:intron 40.0%
TTGGAAAAGCTGGAAATGAG+TGG + chr2_1:11254762-11254781 Msa0194840:CDS 40.0%
ACTGGAAAATGCCGTAAAGA+AGG + chr2_1:11254809-11254828 Msa0194840:CDS 40.0%
! CGCATTTTCAGCCTTCTTTA+CGG - chr2_1:11254823-11254842 None:intergenic 40.0%
!! GATGGTGTTGCAAATGACAT+AGG + chr2_1:11255221-11255240 Msa0194840:intron 40.0%
!! TGGTGTTGCAAATGACATAG+GGG + chr2_1:11255223-11255242 Msa0194840:intron 40.0%
TTTCAAGCATGTACGGTTCA+AGG - chr2_1:11255322-11255341 None:intergenic 40.0%
TTTCCGTGTTTCCCTTCATT+TGG - chr2_1:11255691-11255710 None:intergenic 40.0%
ACAAGAATTATCAGGGAGGA+AGG + chr2_1:11255817-11255836 Msa0194840:CDS 40.0%
CAAGAATTATCAGGGAGGAA+GGG + chr2_1:11255818-11255837 Msa0194840:CDS 40.0%
!!! GAGCCAGGAATTTTAACTAG+TGG + chr2_1:11256144-11256163 Msa0194840:three_prime_UTR 40.0%
CCTCCACTAGTTAAAATTCC+TGG - chr2_1:11256150-11256169 None:intergenic 40.0%
!!! CCAGGAATTTTAACTAGTGG+AGG + chr2_1:11256147-11256166 Msa0194840:three_prime_UTR 40.0%
CGACTCAAGTATGAATCACC+TGG + chr2_1:11254228-11254247 Msa0194840:exon 45.0%
! GCCGTTGTTATTGTCATCCT+TGG + chr2_1:11254361-11254380 Msa0194840:CDS 45.0%
TCGAAAGATAGAGACTCCCA+AGG - chr2_1:11254381-11254400 None:intergenic 45.0%
TGAGCTTGAGCTTCGACTTT+CGG - chr2_1:11254417-11254436 None:intergenic 45.0%
ACTTTGACCGTACAAGGTCA+TGG + chr2_1:11254452-11254471 Msa0194840:CDS 45.0%
GAGTGGAAGTGAAGATTCTG+CGG + chr2_1:11254779-11254798 Msa0194840:CDS 45.0%
AGATTCTGCGGTGGAAATAC+TGG + chr2_1:11254791-11254810 Msa0194840:CDS 45.0%
!! AGTTGATGGGAAGGGTTGAA+GGG - chr2_1:11255068-11255087 None:intergenic 45.0%
!! AAGTTGATGGGAAGGGTTGA+AGG - chr2_1:11255069-11255088 None:intergenic 45.0%
GAACGTGTACCTTGTACAGT+GGG - chr2_1:11255371-11255390 None:intergenic 45.0%
!! AGAGAAAATTCGGTGCAAGG+AGG - chr2_1:11255755-11255774 None:intergenic 45.0%
GTACGAGTTTGACACACTAG+CGG + chr2_1:11256122-11256141 Msa0194840:three_prime_UTR 45.0%
CGACTCAAGTATGAATCACC+TGG + chr2_1:11254228-11254247 Msa0194840:exon 45.0%
! GCCGTTGTTATTGTCATCCT+TGG + chr2_1:11254361-11254380 Msa0194840:CDS 45.0%
TCGAAAGATAGAGACTCCCA+AGG - chr2_1:11254381-11254400 None:intergenic 45.0%
TGAGCTTGAGCTTCGACTTT+CGG - chr2_1:11254417-11254436 None:intergenic 45.0%
ACTTTGACCGTACAAGGTCA+TGG + chr2_1:11254452-11254471 Msa0194840:CDS 45.0%
GAGTGGAAGTGAAGATTCTG+CGG + chr2_1:11254779-11254798 Msa0194840:CDS 45.0%
AGATTCTGCGGTGGAAATAC+TGG + chr2_1:11254791-11254810 Msa0194840:CDS 45.0%
!! AGTTGATGGGAAGGGTTGAA+GGG - chr2_1:11255068-11255087 None:intergenic 45.0%
!! AAGTTGATGGGAAGGGTTGA+AGG - chr2_1:11255069-11255088 None:intergenic 45.0%
GAACGTGTACCTTGTACAGT+GGG - chr2_1:11255371-11255390 None:intergenic 45.0%
!! AGAGAAAATTCGGTGCAAGG+AGG - chr2_1:11255755-11255774 None:intergenic 45.0%
GTACGAGTTTGACACACTAG+CGG + chr2_1:11256122-11256141 Msa0194840:three_prime_UTR 45.0%
! CCTTGTACGGTCAAAGTGTC+CGG - chr2_1:11254449-11254468 None:intergenic 50.0%
!! GCAGCAAGCTTTGGAAAAGC+TGG + chr2_1:11254752-11254771 Msa0194840:intron 50.0%
TGGAAGTGAAGATTCTGCGG+TGG + chr2_1:11254782-11254801 Msa0194840:CDS 50.0%
GGAACGTGTACCTTGTACAG+TGG - chr2_1:11255372-11255391 None:intergenic 50.0%
AAGGAGGATCTTGTACCGCA+AGG - chr2_1:11255739-11255758 None:intergenic 50.0%
! CCTTGTACGGTCAAAGTGTC+CGG - chr2_1:11254449-11254468 None:intergenic 50.0%
!! GCAGCAAGCTTTGGAAAAGC+TGG + chr2_1:11254752-11254771 Msa0194840:intron 50.0%
TGGAAGTGAAGATTCTGCGG+TGG + chr2_1:11254782-11254801 Msa0194840:CDS 50.0%
GGAACGTGTACCTTGTACAG+TGG - chr2_1:11255372-11255391 None:intergenic 50.0%
AAGGAGGATCTTGTACCGCA+AGG - chr2_1:11255739-11255758 None:intergenic 50.0%
! CGGTCAAAGTGTCCGGACTT+GGG - chr2_1:11254442-11254461 None:intergenic 55.0%
! ACGGTCAAAGTGTCCGGACT+TGG - chr2_1:11254443-11254462 None:intergenic 55.0%
CCGGACACTTTGACCGTACA+AGG + chr2_1:11254446-11254465 Msa0194840:CDS 55.0%
CTTCAATTGGCAGGGAGAGC+TGG + chr2_1:11255290-11255309 Msa0194840:intron 55.0%
TTTGACACACTAGCGGAGCC+AGG + chr2_1:11256129-11256148 Msa0194840:three_prime_UTR 55.0%
! CGGTCAAAGTGTCCGGACTT+GGG - chr2_1:11254442-11254461 None:intergenic 55.0%
! ACGGTCAAAGTGTCCGGACT+TGG - chr2_1:11254443-11254462 None:intergenic 55.0%
CCGGACACTTTGACCGTACA+AGG + chr2_1:11254446-11254465 Msa0194840:CDS 55.0%
CTTCAATTGGCAGGGAGAGC+TGG + chr2_1:11255290-11255309 Msa0194840:intron 55.0%
TTTGACACACTAGCGGAGCC+AGG + chr2_1:11256129-11256148 Msa0194840:three_prime_UTR 55.0%
CTCAAGCTCACGCCCAAGTC+CGG + chr2_1:11254427-11254446 Msa0194840:CDS 60.0%
TGCTCGACGCCCACTGTACA+AGG + chr2_1:11255359-11255378 Msa0194840:CDS 60.0%
CTCAAGCTCACGCCCAAGTC+CGG + chr2_1:11254427-11254446 Msa0194840:CDS 60.0%
TGCTCGACGCCCACTGTACA+AGG + chr2_1:11255359-11255378 Msa0194840:CDS 60.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 11254161 11256218 11254161 ID=Msa0194840;Name=Msa0194840
chr2_1 mRNA 11254161 11256218 11254161 ID=Msa0194840-mRNA-1;Parent=Msa0194840;Name=Msa0194840-mRNA-1;_AED=0.01;_eAED=0.01;_QI=77|1|1|1|1|1|4|371|261
chr2_1 exon 11254161 11254627 11254161 ID=Msa0194840-mRNA-1:exon:4978;Parent=Msa0194840-mRNA-1
chr2_1 exon 11254756 11254902 11254756 ID=Msa0194840-mRNA-1:exon:4979;Parent=Msa0194840-mRNA-1
chr2_1 exon 11255303 11255380 11255303 ID=Msa0194840-mRNA-1:exon:4980;Parent=Msa0194840-mRNA-1
chr2_1 exon 11255677 11256218 11255677 ID=Msa0194840-mRNA-1:exon:4981;Parent=Msa0194840-mRNA-1
chr2_1 five_prime_UTR 11254161 11254237 11254161 ID=Msa0194840-mRNA-1:five_prime_utr;Parent=Msa0194840-mRNA-1
chr2_1 CDS 11254238 11254627 11254238 ID=Msa0194840-mRNA-1:cds;Parent=Msa0194840-mRNA-1
chr2_1 CDS 11254756 11254902 11254756 ID=Msa0194840-mRNA-1:cds;Parent=Msa0194840-mRNA-1
chr2_1 CDS 11255303 11255380 11255303 ID=Msa0194840-mRNA-1:cds;Parent=Msa0194840-mRNA-1
chr2_1 CDS 11255677 11255847 11255677 ID=Msa0194840-mRNA-1:cds;Parent=Msa0194840-mRNA-1
chr2_1 three_prime_UTR 11255848 11256218 11255848 ID=Msa0194840-mRNA-1:three_prime_utr;Parent=Msa0194840-mRNA-1
Gene Sequence

>Msa0194840

ATGAATCACCTGGTAGAAAAGTTGATCAATTTATCCCTTTCTTCAAATTATCATGAAGATATGCGAAAATTCATCTTAGCTTTCACATCTAAGTATGAGTTAAATATCAAGATGGCAACACTGGCCGTTGTTATTGTCATCCTTGGGAGTCTCTATCTTTCGACTTATGGGTCAAACCGAAAGTCGAAGCTCAAGCTCACGCCCAAGTCCGGACACTTTGACCGTACAAGGTCATGGATCGTTAGAGAAATTCATAGTGGAAAACCCATATTGGATAGATTGAAAGAGGATTTCAATAAAGCTCGAGTAAATCCTGCAACATTAAAACAAACTAAGAAAGTTTTGAAGGCTTTACTCAAAGAAGAATATCTTGATTTAATAAAGATTCAGCAAGCTTTGGAAAAGCTGGAAATGAGTGGAAGTGAAGATTCTGCGGTGGAAATACTGGAAAATGCCGTAAAGAAGGCTGAAAATGCGAACAAAGCACATGAAGCTTATGAAATTGAAATGTTACTAGTAGAAATGCTCATCTATAAGGGAGAGCTGGACAGTGCCTTGAACCGTACATGCTTGAAAGATGATTCGCTTAAAGATGCTCGACGCCCACTGTACAAGGCAATTATATACCAAATGAAGGGAAACACGGAAAAAACCAATGAATGTTGGAATGAATTCCTTGCGGTACAAGATCCTCCTTGCACCGAATTTTCTCTTTATAAGTTCAAAAAAAATGTGGAACGACTTCAAAGTGCAATACAAGAATTATCAGGGAGGAAGGGACAATAA
Protein sequence

>Msa0194840

MNHLVEKLINLSLSSNYHEDMRKFILAFTSKYELNIKMATLAVVIVILGSLYLSTYGSNRKSKLKLTPKSGHFDRTRSWIVREIHSGKPILDRLKEDFNKARVNPATLKQTKKVLKALLKEEYLDLIKIQQALEKLEMSGSEDSAVEILENAVKKAENANKAHEAYEIEMLLVEMLIYKGELDSALNRTCLKDDSLKDARRPLYKAIIYQMKGNTEKTNECWNEFLAVQDPPCTEFSLYKFKKNVERLQSAIQELSGRKGQ*