AlfalfaGEDB | Msa0534120

M. sativa cultivar zhongmu-4 / Msa0534120

KEGG: K11253
GO: GO:0000785 GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006323 GO:0006325 GO:0006333 GO:0006334 GO:0006996 GO:0008150 GO:0009987 GO:0016043 GO:0022607 GO:0031490 GO:0031491 GO:0031492 GO:0031497 GO:0032991 GO:0032993 GO:0034622 GO:0034728 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0044815 GO:0044877 GO:0051276 GO:0065003 GO:0065004 GO:0071103 GO:0071824 GO:0071840 GO:0097159 GO:1901363
PFAM: PF00125.27

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0534120	KAB5568167.1	94.382	89	4	1	1	89	23	110	2.51e-52	169

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0534120	sp\|P80553\|H3_NARPS	95.402	87	3	1	3	89	51	136	5.01e-54	167

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0534120	A0A5N5NMA5	94.382	89	4	1	1	89	23	110	1.20e-52	169

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0112750	Msa0534120	0.801095	1.058375e-48	-8.615850e-47
Msa0123300	Msa0534120	0.805877	1.079431e-49	-8.615850e-47
Msa0169860	Msa0534120	0.820525	6.551332e-53	-8.615850e-47
Msa0484780	Msa0534120	0.806863	6.689244e-50	-8.615850e-47
Msa0512900	Msa0534120	0.824224	9.061627e-54	-8.615850e-47
Msa0534120	Msa0693380	0.811098	8.295123e-51	-8.615850e-47
Msa0534120	Msa0954920	0.812122	4.968063e-51	-8.615850e-47
Msa0534120	Msa0969450	0.815551	8.726465e-52	-8.615850e-47
Msa0534120	Msa0990350	0.804536	2.060435e-49	-8.615850e-47
Msa0534120	Msa1082500	0.820773	5.745888e-53	-8.615850e-47
Msa0534120	Msa1457430	0.803155	3.989029e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0534120	MtrunA17_Chr4g0054161	95.402	87	3	1	3	89	51	136	3.69e-55	167
Msa0534120	MtrunA17_Chr8g0363741	95.402	87	3	1	3	89	51	136	3.69e-55	167
Msa0534120	MtrunA17_Chr1g0156021	95.402	87	3	1	3	89	51	136	3.69e-55	167
Msa0534120	MtrunA17_Chr4g0054151	95.402	87	3	1	3	89	54	139	3.88e-55	167
Msa0534120	MtrunA17_Chr2g0319131	95.402	87	3	1	3	89	61	146	5.28e-55	167
Msa0534120	MtrunA17_Chr8g0388121	94.253	87	4	1	3	89	51	136	1.26e-54	166
Msa0534120	MtrunA17_Chr5g0410931	94.253	87	4	1	3	89	51	136	5.59e-54	164
Msa0534120	MtrunA17_Chr5g0410951	94.253	87	4	1	3	89	51	136	5.59e-54	164
Msa0534120	MtrunA17_Chr8g0383851	94.253	87	4	1	3	89	51	136	5.59e-54	164
Msa0534120	MtrunA17_Chr8g0383781	94.253	87	4	1	3	89	51	136	5.59e-54	164
Msa0534120	MtrunA17_Chr8g0390361	94.253	87	4	1	3	89	51	136	5.59e-54	164
Msa0534120	MtrunA17_Chr2g0295191	94.253	87	4	1	3	89	51	136	5.59e-54	164
Msa0534120	MtrunA17_Chr7g0237661	94.253	87	4	1	3	89	51	136	5.59e-54	164
Msa0534120	MtrunA17_Chr4g0033041	94.253	87	4	1	3	89	51	136	5.59e-54	164
Msa0534120	MtrunA17_Chr7g0218971	94.253	87	4	1	3	89	51	136	5.59e-54	164
Msa0534120	MtrunA17_Chr4g0046301	94.253	87	4	1	3	89	51	136	5.59e-54	164
Msa0534120	MtrunA17_Chr8g0364801	94.253	87	4	1	3	89	51	136	5.59e-54	164
Msa0534120	MtrunA17_Chr2g0319141	88.506	87	9	1	3	89	51	136	8.01e-51	156
Msa0534120	MtrunA17_Chr2g0319151	88.506	87	9	1	3	89	51	136	8.01e-51	156
Msa0534120	MtrunA17_Chr2g0319121	86.207	87	10	2	3	88	50	135	1.19e-46	145
Msa0534120	MtrunA17_Chr7g0218961	80.460	87	16	1	3	89	28	113	2.37e-42	134
Msa0534120	MtrunA17_Chr7g0222991	75.000	80	20	0	8	87	63	142	7.31e-39	126
Msa0534120	MtrunA17_Chr7g0223001	69.880	83	24	1	6	87	64	146	5.57e-36	119
Msa0534120	MtrunA17_Chr8g0347231	49.412	85	41	2	3	86	42	125	1.12e-20	79.7
Msa0534120	MtrunA17_Chr5g0407311	46.269	67	33	1	3	69	50	113	5.36e-14	62.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0534120	AT4G40030.3	95.402	87	3	1	3	89	51	136	5.09e-55	167
Msa0534120	AT4G40030.1	95.402	87	3	1	3	89	51	136	5.09e-55	167
Msa0534120	AT4G40030.4	95.402	87	3	1	3	89	51	136	5.09e-55	167
Msa0534120	AT5G10980.1	95.402	87	3	1	3	89	51	136	5.09e-55	167
Msa0534120	AT4G40040.2	95.402	87	3	1	3	89	51	136	5.09e-55	167
Msa0534120	AT4G40040.3	95.402	87	3	1	3	89	51	136	5.09e-55	167
Msa0534120	AT4G40040.1	95.402	87	3	1	3	89	51	136	5.09e-55	167
Msa0534120	AT4G40030.2	95.402	87	3	1	3	89	79	164	1.43e-54	167
Msa0534120	AT1G75600.1	93.103	87	5	1	3	89	51	136	3.11e-54	165
Msa0534120	AT1G09200.1	94.253	87	4	1	3	89	51	136	8.61e-54	164
Msa0534120	AT5G65360.1	94.253	87	4	1	3	89	51	136	8.61e-54	164
Msa0534120	AT5G10390.1	94.253	87	4	1	3	89	51	136	8.61e-54	164
Msa0534120	AT5G10400.1	94.253	87	4	1	3	89	51	136	8.61e-54	164
Msa0534120	AT3G27360.1	94.253	87	4	1	3	89	51	136	8.61e-54	164
Msa0534120	AT1G13370.1	91.954	87	6	1	3	89	51	136	8.61e-53	161
Msa0534120	AT1G19890.1	91.954	87	6	1	3	89	52	137	5.54e-52	159
Msa0534120	AT5G65350.1	90.805	87	7	1	3	89	51	136	6.03e-52	159
Msa0534120	AT5G12910.1	75.581	86	20	1	3	88	46	130	1.32e-43	138
Msa0534120	AT1G01370.2	51.724	87	39	2	3	87	89	174	4.73e-20	79.7
Msa0534120	AT1G01370.1	51.724	87	39	2	3	87	89	174	4.73e-20	79.7
Msa0534120	AT1G01370.4	51.724	87	39	2	3	87	89	174	4.73e-20	79.7
Msa0534120	AT1G01370.3	51.724	87	39	2	3	87	89	174	4.73e-20	79.7

Find 24 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTTATGGCGGATTAGTATTT+TGG	0.183704	4_1:+9466706	Msa0534120:three_prime_UTR
CTATGCAGTTCTTGCACTTC+AGG	0.282316	4_1:+9466534	Msa0534120:CDS
TCAAAAGCTCAGTACTATTC+TGG	0.343002	4_1:-9466337	None:intergenic
CATTCGCGGTGAACGTGCTT+AGG	0.355234	4_1:+9466663	Msa0534120:CDS
TACTATTCTGGTAGTACTTA+CGG	0.440467	4_1:-9466325	None:intergenic
TTACCTGGTTGGATTGTTTG+AGG	0.445449	4_1:+9466570	Msa0534120:CDS
GGAAGCAGCTGAGGCTTACC+TGG	0.448380	4_1:+9466555	Msa0534120:CDS
CGAGCAAGCTGAATGTCCTT+TGG	0.451057	4_1:-9466637	None:intergenic
TCACGGACAAGACGCTGAAA+TGG	0.451915	4_1:-9466371	None:intergenic
CAGCTTGCTCGTCGCATTCG+CGG	0.459350	4_1:+9466649	Msa0534120:CDS
TGAAATCTTGAGCAATTTCA+CGG	0.459574	4_1:-9466388	None:intergenic
AGCATGAATTGCACACAGAT+TGG	0.461487	4_1:-9466596	None:intergenic
TGAAATTGCTCAAGATTTCA+AGG	0.466768	4_1:+9466391	Msa0534120:CDS
GTGCAAGAACTGCATAGCTC+TGG	0.487971	4_1:-9466528	None:intergenic
ATTCGCGGTGAACGTGCTTA+GGG	0.501136	4_1:+9466664	Msa0534120:CDS
TGCACTTCAGGAAGCAGCTG+AGG	0.513447	4_1:+9466546	Msa0534120:CDS
GCAGCTGAGGCTTACCTGGT+TGG	0.521313	4_1:+9466559	Msa0534120:CDS
CGCGGTGAACGTGCTTAGGG+TGG	0.589504	4_1:+9466667	Msa0534120:exon
GAACGTGCTTAGGGTGGTGA+AGG	0.623913	4_1:+9466673	Msa0534120:exon
GAGGGTGACAATTATGCCAA+AGG	0.629580	4_1:+9466621	Msa0534120:CDS
GTGTGCAATTCATGCTAAGA+GGG	0.636984	4_1:+9466603	Msa0534120:CDS
GACGCTGAAATGGAAGCTTG+CGG	0.649029	4_1:-9466361	None:intergenic
TGTGTGCAATTCATGCTAAG+AGG	0.657983	4_1:+9466602	Msa0534120:CDS
TGTCCTCAAACAATCCAACC+AGG	0.667495	4_1:-9466573	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATGTTGTTTTATTAATTCTA+TGG	+	chr4_1:9466005-9466024	Msa0534120:intron	15.0%
!!	TCTAAAAACCTAAAAAAATA+GGG	-	chr4_1:9466193-9466212	None:intergenic	15.0%
!!!	ATTTTGGATTCATAATTTTT+AGG	+	chr4_1:9466226-9466245	Msa0534120:intron	15.0%
!!	TGTTAAAAATTGAACATTAT+AGG	-	chr4_1:9466787-9466806	None:intergenic	15.0%
!!!	ATGTTGTTTTATTAATTCTA+TGG	+	chr4_1:9466005-9466024	Msa0534120:intron	15.0%
!!	TCTAAAAACCTAAAAAAATA+GGG	-	chr4_1:9466193-9466212	None:intergenic	15.0%
!!!	ATTTTGGATTCATAATTTTT+AGG	+	chr4_1:9466226-9466245	Msa0534120:intron	15.0%
!!	TGTTAAAAATTGAACATTAT+AGG	-	chr4_1:9466787-9466806	None:intergenic	15.0%
!!	CTAAAAACCTAAAAAAATAG+GGG	-	chr4_1:9466192-9466211	None:intergenic	20.0%
!!!	CTCTAAAAACCTAAAAAAAT+AGG	-	chr4_1:9466194-9466213	None:intergenic	20.0%
!!!	AGAGTTTTGAATTTGAATTT+TGG	+	chr4_1:9466210-9466229	Msa0534120:intron	20.0%
!!!	TTTTAGGAATTTTGAATTTG+CGG	+	chr4_1:9466242-9466261	Msa0534120:intron	20.0%
!!	ACAAATTAAATAAACCAAAG+AGG	-	chr4_1:9466465-9466484	None:intergenic	20.0%
!!!	TTTGATATTGTTGTTATAGA+CGG	+	chr4_1:9466495-9466514	Msa0534120:intron	20.0%
!!!	TTTTAACAACAGACATATTT+TGG	+	chr4_1:9466799-9466818	Msa0534120:three_prime_UTR	20.0%
!!!	AAGTTTGTACTAGATTTTAT+TGG	+	chr4_1:9466846-9466865	Msa0534120:three_prime_UTR	20.0%
!!!	AGTTTGTACTAGATTTTATT+GGG	+	chr4_1:9466847-9466866	Msa0534120:three_prime_UTR	20.0%
!!	CTAAAAACCTAAAAAAATAG+GGG	-	chr4_1:9466192-9466211	None:intergenic	20.0%
!!!	CTCTAAAAACCTAAAAAAAT+AGG	-	chr4_1:9466194-9466213	None:intergenic	20.0%
!!!	AGAGTTTTGAATTTGAATTT+TGG	+	chr4_1:9466210-9466229	Msa0534120:intron	20.0%
!!!	TTTTAGGAATTTTGAATTTG+CGG	+	chr4_1:9466242-9466261	Msa0534120:intron	20.0%
!!	ACAAATTAAATAAACCAAAG+AGG	-	chr4_1:9466465-9466484	None:intergenic	20.0%
!!!	TTTGATATTGTTGTTATAGA+CGG	+	chr4_1:9466495-9466514	Msa0534120:intron	20.0%
!!!	TTTTAACAACAGACATATTT+TGG	+	chr4_1:9466799-9466818	Msa0534120:three_prime_UTR	20.0%
!!!	AAGTTTGTACTAGATTTTAT+TGG	+	chr4_1:9466846-9466865	Msa0534120:three_prime_UTR	20.0%
!!!	AGTTTGTACTAGATTTTATT+GGG	+	chr4_1:9466847-9466866	Msa0534120:three_prime_UTR	20.0%
!	TATCCATTGTAATTTCTTGT+TGG	+	chr4_1:9465774-9465793	Msa0534120:intron	25.0%
!	TCATTTGCTTGGATTAATTA+TGG	+	chr4_1:9465833-9465852	Msa0534120:intron	25.0%
!	AACAACAATTGAATAGATCA+AGG	-	chr4_1:9465973-9465992	None:intergenic	25.0%
!!!	TGTTTTATTAATTCTATGGC+AGG	+	chr4_1:9466009-9466028	Msa0534120:intron	25.0%
!!	ATTTGCGGTTTATGATTTTT+AGG	+	chr4_1:9466257-9466276	Msa0534120:intron	25.0%
!!!	TTAGTATTTTGGAAGGATTT+AGG	+	chr4_1:9466717-9466736	Msa0534120:three_prime_UTR	25.0%
!!!	TAGTATTTTGGAAGGATTTA+GGG	+	chr4_1:9466718-9466737	Msa0534120:three_prime_UTR	25.0%
!	TATCCATTGTAATTTCTTGT+TGG	+	chr4_1:9465774-9465793	Msa0534120:intron	25.0%
!	TCATTTGCTTGGATTAATTA+TGG	+	chr4_1:9465833-9465852	Msa0534120:intron	25.0%
!	AACAACAATTGAATAGATCA+AGG	-	chr4_1:9465973-9465992	None:intergenic	25.0%
!!!	TGTTTTATTAATTCTATGGC+AGG	+	chr4_1:9466009-9466028	Msa0534120:intron	25.0%
!!	ATTTGCGGTTTATGATTTTT+AGG	+	chr4_1:9466257-9466276	Msa0534120:intron	25.0%
!!!	TTAGTATTTTGGAAGGATTT+AGG	+	chr4_1:9466717-9466736	Msa0534120:three_prime_UTR	25.0%
!!!	TAGTATTTTGGAAGGATTTA+GGG	+	chr4_1:9466718-9466737	Msa0534120:three_prime_UTR	25.0%
!!!	TGACCATCTTGTTACTTTTA+TGG	+	chr4_1:9465664-9465683	Msa0534120:intron	30.0%
	GAATATGTCAAAACACTATC+AGG	-	chr4_1:9465692-9465711	None:intergenic	30.0%
!!	AGCAATGCACTTTTTGAATA+GGG	-	chr4_1:9465715-9465734	None:intergenic	30.0%
!!	AAGCAATGCACTTTTTGAAT+AGG	-	chr4_1:9465716-9465735	None:intergenic	30.0%
	CAACCAACAAGAAATTACAA+TGG	-	chr4_1:9465780-9465799	None:intergenic	30.0%
!	TTGATTTTGGATAGGATAGA+GGG	-	chr4_1:9465928-9465947	None:intergenic	30.0%
!	CATCAAACTTGATTTTGGAT+AGG	-	chr4_1:9465936-9465955	None:intergenic	30.0%
	CTATCCAAAATCAAGTTTGA+TGG	+	chr4_1:9465934-9465953	Msa0534120:intron	30.0%
!	ATGACCATCAAACTTGATTT+TGG	-	chr4_1:9465941-9465960	None:intergenic	30.0%
!!	CTATTCAATTGTTGTTTGCA+AGG	+	chr4_1:9465977-9465996	Msa0534120:intron	30.0%
!!!	TCGTTTTCCCCTATTTTTTT+AGG	+	chr4_1:9466182-9466201	Msa0534120:intron	30.0%
	TACTATTCTGGTAGTACTTA+CGG	-	chr4_1:9466328-9466347	None:intergenic	30.0%
	TGAAATCTTGAGCAATTTCA+CGG	-	chr4_1:9466391-9466410	None:intergenic	30.0%
	TGAAATTGCTCAAGATTTCA+AGG	+	chr4_1:9466391-9466410	Msa0534120:CDS	30.0%
!!!	TGACCATCTTGTTACTTTTA+TGG	+	chr4_1:9465664-9465683	Msa0534120:intron	30.0%
	GAATATGTCAAAACACTATC+AGG	-	chr4_1:9465692-9465711	None:intergenic	30.0%
!!	AGCAATGCACTTTTTGAATA+GGG	-	chr4_1:9465715-9465734	None:intergenic	30.0%
!!	AAGCAATGCACTTTTTGAAT+AGG	-	chr4_1:9465716-9465735	None:intergenic	30.0%
	CAACCAACAAGAAATTACAA+TGG	-	chr4_1:9465780-9465799	None:intergenic	30.0%
!	TTGATTTTGGATAGGATAGA+GGG	-	chr4_1:9465928-9465947	None:intergenic	30.0%
!	CATCAAACTTGATTTTGGAT+AGG	-	chr4_1:9465936-9465955	None:intergenic	30.0%
	CTATCCAAAATCAAGTTTGA+TGG	+	chr4_1:9465934-9465953	Msa0534120:intron	30.0%
!	ATGACCATCAAACTTGATTT+TGG	-	chr4_1:9465941-9465960	None:intergenic	30.0%
!!	CTATTCAATTGTTGTTTGCA+AGG	+	chr4_1:9465977-9465996	Msa0534120:intron	30.0%
!!!	TCGTTTTCCCCTATTTTTTT+AGG	+	chr4_1:9466182-9466201	Msa0534120:intron	30.0%
	TACTATTCTGGTAGTACTTA+CGG	-	chr4_1:9466328-9466347	None:intergenic	30.0%
	TGAAATCTTGAGCAATTTCA+CGG	-	chr4_1:9466391-9466410	None:intergenic	30.0%
	TGAAATTGCTCAAGATTTCA+AGG	+	chr4_1:9466391-9466410	Msa0534120:CDS	30.0%
	GAGCCATAAAAGTAACAAGA+TGG	-	chr4_1:9465670-9465689	None:intergenic	35.0%
!!	GCAATGCACTTTTTGAATAG+GGG	-	chr4_1:9465714-9465733	None:intergenic	35.0%
	TCAAGAGAACTTCATTTGCT+TGG	+	chr4_1:9465822-9465841	Msa0534120:intron	35.0%
!	CTTGATTTTGGATAGGATAG+AGG	-	chr4_1:9465929-9465948	None:intergenic	35.0%
	CAATTGAATAGATCAAGGCA+AGG	-	chr4_1:9465968-9465987	None:intergenic	35.0%
	TATTAATTCTATGGCAGGAG+TGG	+	chr4_1:9466014-9466033	Msa0534120:intron	35.0%
	TTGTCAAAGCATATGAGAAC+TGG	+	chr4_1:9466044-9466063	Msa0534120:intron	35.0%
!	CATATGAGAACTGGAAGTAT+TGG	+	chr4_1:9466053-9466072	Msa0534120:intron	35.0%
	TTGTCCTCAAGGTTGTAAAA+TGG	-	chr4_1:9466082-9466101	None:intergenic	35.0%
	TCAAAAGCTCAGTACTATTC+TGG	-	chr4_1:9466340-9466359	None:intergenic	35.0%
	TGTGAAATCTTGTTCCTCTT+TGG	+	chr4_1:9466448-9466467	Msa0534120:intron	35.0%
!!!	GTTATGGCGGATTAGTATTT+TGG	+	chr4_1:9466706-9466725	Msa0534120:three_prime_UTR	35.0%
	GAGCCATAAAAGTAACAAGA+TGG	-	chr4_1:9465670-9465689	None:intergenic	35.0%
!!	GCAATGCACTTTTTGAATAG+GGG	-	chr4_1:9465714-9465733	None:intergenic	35.0%
	TCAAGAGAACTTCATTTGCT+TGG	+	chr4_1:9465822-9465841	Msa0534120:intron	35.0%
!	CTTGATTTTGGATAGGATAG+AGG	-	chr4_1:9465929-9465948	None:intergenic	35.0%
	CAATTGAATAGATCAAGGCA+AGG	-	chr4_1:9465968-9465987	None:intergenic	35.0%
	TATTAATTCTATGGCAGGAG+TGG	+	chr4_1:9466014-9466033	Msa0534120:intron	35.0%
	TTGTCAAAGCATATGAGAAC+TGG	+	chr4_1:9466044-9466063	Msa0534120:intron	35.0%
!	CATATGAGAACTGGAAGTAT+TGG	+	chr4_1:9466053-9466072	Msa0534120:intron	35.0%
	TTGTCCTCAAGGTTGTAAAA+TGG	-	chr4_1:9466082-9466101	None:intergenic	35.0%
	TCAAAAGCTCAGTACTATTC+TGG	-	chr4_1:9466340-9466359	None:intergenic	35.0%
	TGTGAAATCTTGTTCCTCTT+TGG	+	chr4_1:9466448-9466467	Msa0534120:intron	35.0%
!!!	GTTATGGCGGATTAGTATTT+TGG	+	chr4_1:9466706-9466725	Msa0534120:three_prime_UTR	35.0%
	TTCATCTTGCCTCAGAAACT+TGG	+	chr4_1:9465734-9465753	Msa0534120:intron	40.0%
!	GCTACCATTTTACAACCTTG+AGG	+	chr4_1:9466075-9466094	Msa0534120:intron	40.0%
!!	TTTTTGACTCCTCCAGTAGT+AGG	-	chr4_1:9466119-9466138	None:intergenic	40.0%
	TTACCTGGTTGGATTGTTTG+AGG	+	chr4_1:9466570-9466589	Msa0534120:CDS	40.0%
	AGCATGAATTGCACACAGAT+TGG	-	chr4_1:9466599-9466618	None:intergenic	40.0%
	TGTGTGCAATTCATGCTAAG+AGG	+	chr4_1:9466602-9466621	Msa0534120:CDS	40.0%
	GTGTGCAATTCATGCTAAGA+GGG	+	chr4_1:9466603-9466622	Msa0534120:CDS	40.0%
!!!	TGAAGGTGCTTTTAGGGTTA+TGG	+	chr4_1:9466690-9466709	Msa0534120:three_prime_UTR	40.0%
!!!	TGGCGGATTAGTATTTTGGA+AGG	+	chr4_1:9466710-9466729	Msa0534120:three_prime_UTR	40.0%
	TTCATCTTGCCTCAGAAACT+TGG	+	chr4_1:9465734-9465753	Msa0534120:intron	40.0%
!	GCTACCATTTTACAACCTTG+AGG	+	chr4_1:9466075-9466094	Msa0534120:intron	40.0%
!!	TTTTTGACTCCTCCAGTAGT+AGG	-	chr4_1:9466119-9466138	None:intergenic	40.0%
	TTACCTGGTTGGATTGTTTG+AGG	+	chr4_1:9466570-9466589	Msa0534120:CDS	40.0%
	AGCATGAATTGCACACAGAT+TGG	-	chr4_1:9466599-9466618	None:intergenic	40.0%
	TGTGTGCAATTCATGCTAAG+AGG	+	chr4_1:9466602-9466621	Msa0534120:CDS	40.0%
	GTGTGCAATTCATGCTAAGA+GGG	+	chr4_1:9466603-9466622	Msa0534120:CDS	40.0%
!!!	TGAAGGTGCTTTTAGGGTTA+TGG	+	chr4_1:9466690-9466709	Msa0534120:three_prime_UTR	40.0%
!!!	TGGCGGATTAGTATTTTGGA+AGG	+	chr4_1:9466710-9466729	Msa0534120:three_prime_UTR	40.0%
	CCTCAGAAACTTGGACATGT+GGG	+	chr4_1:9465743-9465762	Msa0534120:intron	45.0%
	TTACAACCTTGAGGACAAGC+AGG	+	chr4_1:9466084-9466103	Msa0534120:intron	45.0%
	AGATTTCCTGCTTGTCCTCA+AGG	-	chr4_1:9466093-9466112	None:intergenic	45.0%
	AGGAAATCTGCTCCTACTAC+TGG	+	chr4_1:9466104-9466123	Msa0534120:intron	45.0%
	AAATCTGCTCCTACTACTGG+AGG	+	chr4_1:9466107-9466126	Msa0534120:intron	45.0%
	ACGAAATTACCGAAGTGCGA+CGG	-	chr4_1:9466166-9466185	None:intergenic	45.0%
	CTATGCAGTTCTTGCACTTC+AGG	+	chr4_1:9466534-9466553	Msa0534120:CDS	45.0%
	TGTCCTCAAACAATCCAACC+AGG	-	chr4_1:9466576-9466595	None:intergenic	45.0%
	GAGGGTGACAATTATGCCAA+AGG	+	chr4_1:9466621-9466640	Msa0534120:CDS	45.0%
!!!	AGGTGCTTTTAGGGTTATGG+CGG	+	chr4_1:9466693-9466712	Msa0534120:three_prime_UTR	45.0%
	CCTCAGAAACTTGGACATGT+GGG	+	chr4_1:9465743-9465762	Msa0534120:intron	45.0%
	TTACAACCTTGAGGACAAGC+AGG	+	chr4_1:9466084-9466103	Msa0534120:intron	45.0%
	AGATTTCCTGCTTGTCCTCA+AGG	-	chr4_1:9466093-9466112	None:intergenic	45.0%
	AGGAAATCTGCTCCTACTAC+TGG	+	chr4_1:9466104-9466123	Msa0534120:intron	45.0%
	AAATCTGCTCCTACTACTGG+AGG	+	chr4_1:9466107-9466126	Msa0534120:intron	45.0%
	ACGAAATTACCGAAGTGCGA+CGG	-	chr4_1:9466166-9466185	None:intergenic	45.0%
	CTATGCAGTTCTTGCACTTC+AGG	+	chr4_1:9466534-9466553	Msa0534120:CDS	45.0%
	TGTCCTCAAACAATCCAACC+AGG	-	chr4_1:9466576-9466595	None:intergenic	45.0%
	GAGGGTGACAATTATGCCAA+AGG	+	chr4_1:9466621-9466640	Msa0534120:CDS	45.0%
!!!	AGGTGCTTTTAGGGTTATGG+CGG	+	chr4_1:9466693-9466712	Msa0534120:three_prime_UTR	45.0%
	GCCTCAGAAACTTGGACATG+TGG	+	chr4_1:9465742-9465761	Msa0534120:intron	50.0%
	CCCACATGTCCAAGTTTCTG+AGG	-	chr4_1:9465746-9465765	None:intergenic	50.0%
	GACGCTGAAATGGAAGCTTG+CGG	-	chr4_1:9466364-9466383	None:intergenic	50.0%
	TCACGGACAAGACGCTGAAA+TGG	-	chr4_1:9466374-9466393	None:intergenic	50.0%
	GTGCAAGAACTGCATAGCTC+TGG	-	chr4_1:9466531-9466550	None:intergenic	50.0%
	CGAGCAAGCTGAATGTCCTT+TGG	-	chr4_1:9466640-9466659	None:intergenic	50.0%
	ATTCGCGGTGAACGTGCTTA+GGG	+	chr4_1:9466664-9466683	Msa0534120:CDS	50.0%
!!!	AGGGTGGTGAAGGTGCTTTT+AGG	+	chr4_1:9466683-9466702	Msa0534120:exon	50.0%
!!!	GGGTGGTGAAGGTGCTTTTA+GGG	+	chr4_1:9466684-9466703	Msa0534120:exon	50.0%
	GCCTCAGAAACTTGGACATG+TGG	+	chr4_1:9465742-9465761	Msa0534120:intron	50.0%
	CCCACATGTCCAAGTTTCTG+AGG	-	chr4_1:9465746-9465765	None:intergenic	50.0%
	GACGCTGAAATGGAAGCTTG+CGG	-	chr4_1:9466364-9466383	None:intergenic	50.0%
	TCACGGACAAGACGCTGAAA+TGG	-	chr4_1:9466374-9466393	None:intergenic	50.0%
	GTGCAAGAACTGCATAGCTC+TGG	-	chr4_1:9466531-9466550	None:intergenic	50.0%
	CGAGCAAGCTGAATGTCCTT+TGG	-	chr4_1:9466640-9466659	None:intergenic	50.0%
	ATTCGCGGTGAACGTGCTTA+GGG	+	chr4_1:9466664-9466683	Msa0534120:CDS	50.0%
!!!	AGGGTGGTGAAGGTGCTTTT+AGG	+	chr4_1:9466683-9466702	Msa0534120:exon	50.0%
!!!	GGGTGGTGAAGGTGCTTTTA+GGG	+	chr4_1:9466684-9466703	Msa0534120:exon	50.0%
!!	GTTCTAGGACGGTAACGGTG+AGG	-	chr4_1:9466144-9466163	None:intergenic	55.0%
!!	CGACGGTTCTAGGACGGTAA+CGG	-	chr4_1:9466149-9466168	None:intergenic	55.0%
!!	GAAGTGCGACGGTTCTAGGA+CGG	-	chr4_1:9466155-9466174	None:intergenic	55.0%
	GTCCTAGAACCGTCGCACTT+CGG	+	chr4_1:9466154-9466173	Msa0534120:intron	55.0%
	TACCGAAGTGCGACGGTTCT+AGG	-	chr4_1:9466159-9466178	None:intergenic	55.0%
	TGCACTTCAGGAAGCAGCTG+AGG	+	chr4_1:9466546-9466565	Msa0534120:CDS	55.0%
	CATTCGCGGTGAACGTGCTT+AGG	+	chr4_1:9466663-9466682	Msa0534120:CDS	55.0%
	GAACGTGCTTAGGGTGGTGA+AGG	+	chr4_1:9466673-9466692	Msa0534120:exon	55.0%
!!	GTTCTAGGACGGTAACGGTG+AGG	-	chr4_1:9466144-9466163	None:intergenic	55.0%
!!	CGACGGTTCTAGGACGGTAA+CGG	-	chr4_1:9466149-9466168	None:intergenic	55.0%
!!	GAAGTGCGACGGTTCTAGGA+CGG	-	chr4_1:9466155-9466174	None:intergenic	55.0%
	GTCCTAGAACCGTCGCACTT+CGG	+	chr4_1:9466154-9466173	Msa0534120:intron	55.0%
	TACCGAAGTGCGACGGTTCT+AGG	-	chr4_1:9466159-9466178	None:intergenic	55.0%
	TGCACTTCAGGAAGCAGCTG+AGG	+	chr4_1:9466546-9466565	Msa0534120:CDS	55.0%
	CATTCGCGGTGAACGTGCTT+AGG	+	chr4_1:9466663-9466682	Msa0534120:CDS	55.0%
	GAACGTGCTTAGGGTGGTGA+AGG	+	chr4_1:9466673-9466692	Msa0534120:exon	55.0%
	GGAAGCAGCTGAGGCTTACC+TGG	+	chr4_1:9466555-9466574	Msa0534120:CDS	60.0%
	GCAGCTGAGGCTTACCTGGT+TGG	+	chr4_1:9466559-9466578	Msa0534120:CDS	60.0%
!	CAGCTTGCTCGTCGCATTCG+CGG	+	chr4_1:9466649-9466668	Msa0534120:CDS	60.0%
	GGAAGCAGCTGAGGCTTACC+TGG	+	chr4_1:9466555-9466574	Msa0534120:CDS	60.0%
	GCAGCTGAGGCTTACCTGGT+TGG	+	chr4_1:9466559-9466578	Msa0534120:CDS	60.0%
!	CAGCTTGCTCGTCGCATTCG+CGG	+	chr4_1:9466649-9466668	Msa0534120:CDS	60.0%
	CGCGGTGAACGTGCTTAGGG+TGG	+	chr4_1:9466667-9466686	Msa0534120:exon	65.0%
	CGCGGTGAACGTGCTTAGGG+TGG	+	chr4_1:9466667-9466686	Msa0534120:exon	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4_1	gene	9465636	9466903	9465636	ID=Msa0534120;Name=Msa0534120
chr4_1	mRNA	9465636	9466903	9465636	ID=Msa0534120-mRNA-1;Parent=Msa0534120;Name=Msa0534120-mRNA-1;_AED=0.39;_eAED=0.39;_QI=0\|0.5\|0.66\|0.66\|1\|1\|3\|219\|89
chr4_1	exon	9465636	9465640	9465636	ID=Msa0534120-mRNA-1:exon:3080;Parent=Msa0534120-mRNA-1
chr4_1	exon	9466319	9466412	9466319	ID=Msa0534120-mRNA-1:exon:3081;Parent=Msa0534120-mRNA-1
chr4_1	exon	9466514	9466903	9466514	ID=Msa0534120-mRNA-1:exon:3082;Parent=Msa0534120-mRNA-1
chr4_1	CDS	9465636	9465640	9465636	ID=Msa0534120-mRNA-1:cds;Parent=Msa0534120-mRNA-1
chr4_1	CDS	9466319	9466412	9466319	ID=Msa0534120-mRNA-1:cds;Parent=Msa0534120-mRNA-1
chr4_1	CDS	9466514	9466684	9466514	ID=Msa0534120-mRNA-1:cds;Parent=Msa0534120-mRNA-1
chr4_1	three_prime_UTR	9466685	9466903	9466685	ID=Msa0534120-mRNA-1:three_prime_utr;Parent=Msa0534120-mRNA-1

Gene Sequence	>Msa0534120 ATGGGTGAGATCCGTAAGTACTACCAGAATAGTACTGAGCTTTTGATCCGCAAGCTTCCATTTCAGCGTCTTGTCCGTGAAATTGCTCAAGATTTCAAGACGGATCTGAAATTCCAGAGCTATGCAGTTCTTGCACTTCAGGAAGCAGCTGAGGCTTACCTGGTTGGATTGTTTGAGGACACCAATCTGTGTGCAATTCATGCTAAGAGGGTGACAATTATGCCAAAGGACATTCAGCTTGCTCGTCGCATTCGCGGTGAACGTGCTTAG
Protein sequence	>Msa0534120 MGEIRKYYQNSTELLIRKLPFQRLVREIAQDFKTDLKFQSYAVLALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA*