AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0545930

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0545930 MtrunA17_Chr4g0027001 90.533 169 15 1 1 168 1 169 5.48e-107 305
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0545930 AT3G44620.1 61.111 162 47 6 14 168 18 170 1.12e-55 176
Msa0545930 AT3G44620.2 61.111 162 47 6 14 168 18 170 2.51e-55 175

Find 75 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCATTTGTGACTTAATTCT+TGG 0.152433 4_1:-36940967 None:intergenic
AATTCACATTGTCATATTAT+TGG 0.172149 4_1:-36941273 None:intergenic
AAGGAAATGGAAGAAGATTT+TGG 0.179286 4_1:-36938465 None:intergenic
AAATAAATGGAAAAGAATTA+AGG 0.204380 4_1:+36941205 Msa0545930:three_prime_UTR
TTTGAAGCTGACCTCATTCT+TGG 0.214058 4_1:-36939000 None:intergenic
TGGGAATATTTAACATGTTC+AGG 0.219183 4_1:+36941164 Msa0545930:three_prime_UTR
TCAAAATATCACTTGATAAT+TGG 0.220024 4_1:-36941132 None:intergenic
AATCCATGGCTTCTATATAT+TGG 0.226052 4_1:+36940924 Msa0545930:three_prime_UTR
GGATCTTCTGGCTATTAAAA+AGG 0.238113 4_1:-36940829 None:intergenic
TATATAGAAGCCATGGATTT+AGG 0.251890 4_1:-36940920 None:intergenic
GGCAAAATGGTAAATTAATA+AGG 0.261064 4_1:-36938368 None:intergenic
ATCATTGCTGGATAGCATTT+TGG 0.270039 4_1:+36940775 Msa0545930:three_prime_UTR
GAAATGAAGAATGGGACATT+TGG 0.317552 4_1:-36938417 None:intergenic
AAATGAAGAATGGGACATTT+GGG 0.319906 4_1:-36938416 None:intergenic
AATCCCAAGATTGGATCTTC+TGG 0.327565 4_1:-36940841 None:intergenic
TTGGGTTTGGACAGAGGAAA+TGG 0.356282 4_1:-36938398 None:intergenic
ATCCATGGCTTCTATATATT+GGG 0.357423 4_1:+36940925 Msa0545930:three_prime_UTR
ACCTGAAGATGCCCACAAAA+AGG 0.360016 4_1:+36939265 Msa0545930:three_prime_UTR
TCTGTTCTCTTTGTATGTCT+TGG 0.361485 4_1:+36938564 Msa0545930:CDS
TTCTCCAATTCAAGCTTCAA+TGG 0.372783 4_1:+36938503 Msa0545930:CDS
ACAAAATCAGAAGGCTTGAT+TGG 0.384101 4_1:-36939057 None:intergenic
AATAAATGGAAAAGAATTAA+GGG 0.397442 4_1:+36941206 Msa0545930:three_prime_UTR
TTCAGATAACTTCAATTTCA+AGG 0.407479 4_1:+36939034 Msa0545930:CDS
AAGAATGGGACATTTGGGTT+TGG 0.415900 4_1:-36938411 None:intergenic
TTGTGGGCATCTTCAGGTAA+AGG 0.416440 4_1:-36939260 None:intergenic
GAAGCCATTGAAGCTTGAAT+TGG 0.416893 4_1:-36938507 None:intergenic
TGTGGGCATCTTCAGGTAAA+GGG 0.416937 4_1:-36939259 None:intergenic
AAATGGGAAGAGTGGCAAAA+TGG 0.422646 4_1:-36938381 None:intergenic
TATTCCTCTGCAACCAATTG+AGG 0.423028 4_1:-36938312 None:intergenic
AGATGCTTGTGAATCATTGC+TGG 0.423871 4_1:+36940763 Msa0545930:three_prime_UTR
GTGAAAACACCTTCAGCAGC+TGG 0.442462 4_1:-36938603 None:intergenic
GTTTGATCTTATTCTTGCTA+TGG 0.444378 4_1:+36939083 Msa0545930:CDS
TGGGATTAAGCTGTAGAGAT+TGG 0.445393 4_1:+36940857 Msa0545930:three_prime_UTR
CTACAGCTTAATCCCAAGAT+TGG 0.448897 4_1:-36940850 None:intergenic
ATAGCCAGAAGATCCAATCT+TGG 0.451431 4_1:+36940837 Msa0545930:three_prime_UTR
GTTTCATTGTCCTAAATCCA+TGG 0.454744 4_1:+36940910 Msa0545930:three_prime_UTR
ATTGATTCTGCTGGTACAAT+CGG 0.457565 4_1:+36938666 Msa0545930:CDS
ATCTAGTGGAGCTCCTCAAT+TGG 0.463965 4_1:+36938299 Msa0545930:five_prime_UTR
TTGTGACTTAATTCTTGGAT+AGG 0.464447 4_1:-36940962 None:intergenic
TAGCCAGAAGATCCAATCTT+GGG 0.466253 4_1:+36940838 Msa0545930:three_prime_UTR
GGGAATATTTAACATGTTCA+GGG 0.474221 4_1:+36941165 Msa0545930:three_prime_UTR
AAGTTCAGAATTGATTCTGC+TGG 0.475953 4_1:+36938657 Msa0545930:CDS
GAAGTCCCGGATCCTTACTA+TGG 0.479762 4_1:+36939736 Msa0545930:three_prime_UTR
TGCTGAGTCTCAGTATTCAA+TGG 0.480517 4_1:-36941028 None:intergenic
CTGTTGACATGCACATTACA+AGG 0.481277 4_1:+36941083 Msa0545930:three_prime_UTR
GGTCCACCATAGTAAGGATC+CGG 0.483919 4_1:-36939742 None:intergenic
CCTTGAGGTCCACCATAGTA+AGG 0.486542 4_1:-36939748 None:intergenic
CCTTACTATGGTGGACCTCA+AGG 0.500453 4_1:+36939748 Msa0545930:three_prime_UTR
CACTTGATAATTGGAAGGAG+AGG 0.516686 4_1:-36941123 None:intergenic
GCATGATGAAACTGAAGTCC+CGG 0.528940 4_1:+36939723 Msa0545930:three_prime_UTR
TGGGTTTGGACAGAGGAAAT+GGG 0.529010 4_1:-36938397 None:intergenic
GCTATGGACAAGCAAAATAG+AGG 0.529514 4_1:+36939099 Msa0545930:CDS
GATTAAGCTGTAGAGATTGG+CGG 0.539780 4_1:+36940860 Msa0545930:three_prime_UTR
TGTGTGGAAGAAATGAAGAA+TGG 0.543866 4_1:-36938426 None:intergenic
TTCCCAATATATAGAAGCCA+TGG 0.544506 4_1:-36940927 None:intergenic
ACTTGATAATTGGAAGGAGA+GGG 0.549667 4_1:-36941122 None:intergenic
CATACAAAGAGAACAGAGAA+TGG 0.556968 4_1:-36938558 None:intergenic
GTGTGGAAGAAATGAAGAAT+GGG 0.556979 4_1:-36938425 None:intergenic
AATATCACTTGATAATTGGA+AGG 0.564784 4_1:-36941128 None:intergenic
AGCTCCTCAATTGGTTGCAG+AGG 0.565264 4_1:+36938308 Msa0545930:five_prime_UTR
TGTAGAAGCCCAGCTGCTGA+AGG 0.566668 4_1:+36938594 Msa0545930:CDS
ACTTAATTCTTGGATAGGCA+TGG 0.571692 4_1:-36940957 None:intergenic
TGAAAACACCTTCAGCAGCT+GGG 0.579306 4_1:-36938602 None:intergenic
GTCCACCATAGTAAGGATCC+GGG 0.586473 4_1:-36939741 None:intergenic
ATAAATGGAAAAGAATTAAG+GGG 0.586512 4_1:+36941207 Msa0545930:three_prime_UTR
GGACATTTGGGTTTGGACAG+AGG 0.587193 4_1:-36938404 None:intergenic
TTGAATGTAAAGTTATCTAG+TGG 0.587388 4_1:+36938285 Msa0545930:five_prime_UTR
GACAGAGGAAATGGGAAGAG+TGG 0.588053 4_1:-36938389 None:intergenic
TCAAACTCAACAAAATCAGA+AGG 0.591117 4_1:-36939066 None:intergenic
ATGAAGCTGATCCAAGAATG+AGG 0.615117 4_1:+36938989 Msa0545930:CDS
GTCCCGGATCCTTACTATGG+TGG 0.618032 4_1:+36939739 Msa0545930:three_prime_UTR
ACTCAGCAGATTATATAGCA+TGG 0.620807 4_1:+36941043 Msa0545930:three_prime_UTR
ATGAAGACACTATACACTGA+CGG 0.640732 4_1:+36938339 Msa0545930:CDS
TATACCTTCTCAAAACCTTG+AGG 0.655564 4_1:-36939763 None:intergenic
TTTAGGACAATGAAACAACA+AGG 0.714835 4_1:-36940903 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATGAAAAAAATTGTTTTTT+TGG + chr4_1:36938904-36938923 Msa0545930:intron 10.0%
!!! ATGAAAAAAATTGTTTTTTT+GGG + chr4_1:36938905-36938924 Msa0545930:intron 10.0%
!!! AAAAGTTGATTTTTTTATGA+TGG + chr4_1:36938754-36938773 Msa0545930:intron 15.0%
!!! AAAGTTGATTTTTTTATGAT+GGG + chr4_1:36938755-36938774 Msa0545930:intron 15.0%
!!! TCTTTTTAGTAAATTGTAAT+GGG + chr4_1:36939176-36939195 Msa0545930:intron 15.0%
!! AAATAAATGGAAAAGAATTA+AGG + chr4_1:36941205-36941224 Msa0545930:three_prime_UTR 15.0%
!! AATAAATGGAAAAGAATTAA+GGG + chr4_1:36941206-36941225 Msa0545930:three_prime_UTR 15.0%
!!! AGAAAAGTGATTAATTTTGA+AGG - chr4_1:36938487-36938506 None:intergenic 20.0%
!!! AAGTTGATTTTTTTTGTTGA+AGG + chr4_1:36938963-36938982 Msa0545930:intron 20.0%
!!! AGTTGATTTTTTTTGTTGAA+GGG + chr4_1:36938964-36938983 Msa0545930:intron 20.0%
!!! CTCTTTTTAGTAAATTGTAA+TGG + chr4_1:36939175-36939194 Msa0545930:intron 20.0%
!!! TTAGTTTTTTTCTTTCTTTC+TGG + chr4_1:36939529-36939548 Msa0545930:intron 20.0%
!! GTCTAAAAAAAACTCAAATT+AGG - chr4_1:36939559-36939578 None:intergenic 20.0%
!!! TTTTTAGACAACCTAAATTA+AGG + chr4_1:36939570-36939589 Msa0545930:intron 20.0%
!! GTATATTCTTCATGATTTAT+TGG + chr4_1:36939781-36939800 Msa0545930:intron 20.0%
!!! GATAATATTTTCTTTGTCAT+TGG + chr4_1:36940180-36940199 Msa0545930:intron 20.0%
!!! CTTCTAAAGACATTTTAAAT+TGG + chr4_1:36940454-36940473 Msa0545930:intron 20.0%
!! TACTGTTTATAAACTATTTC+TGG - chr4_1:36940557-36940576 None:intergenic 20.0%
!!! TGTTTTCAAATGATAATATG+TGG - chr4_1:36940656-36940675 None:intergenic 20.0%
!!! TCAAAATATCACTTGATAAT+TGG - chr4_1:36941135-36941154 None:intergenic 20.0%
!!! GTATGAGAGTTTTAAATAAA+TGG + chr4_1:36941192-36941211 Msa0545930:three_prime_UTR 20.0%
!! ATAAATGGAAAAGAATTAAG+GGG + chr4_1:36941207-36941226 Msa0545930:three_prime_UTR 20.0%
!! AATTCACATTGTCATATTAT+TGG - chr4_1:36941276-36941295 None:intergenic 20.0%
! TTGAATGTAAAGTTATCTAG+TGG + chr4_1:36938285-36938304 Msa0545930:five_prime_UTR 25.0%
! GGCAAAATGGTAAATTAATA+AGG - chr4_1:36938371-36938390 None:intergenic 25.0%
!!! AGATTTTGGAGTTAATTTTC+TGG - chr4_1:36938454-36938473 None:intergenic 25.0%
!!! GTGATTAATTTTGAAGGAAA+TGG - chr4_1:36938481-36938500 None:intergenic 25.0%
!! AATTTCGGAATTGATTCTAT+TGG + chr4_1:36938815-36938834 Msa0545930:intron 25.0%
! TACAATCAATTATCATGAAG+TGG + chr4_1:36938838-36938857 Msa0545930:intron 25.0%
! ACAATCAATTATCATGAAGT+GGG + chr4_1:36938839-36938858 Msa0545930:intron 25.0%
!!! TTTTTGGGTTGTTTTTATGA+TGG + chr4_1:36938920-36938939 Msa0545930:intron 25.0%
!!! TTTTGGGTTGTTTTTATGAT+GGG + chr4_1:36938921-36938940 Msa0545930:intron 25.0%
! TTCAGATAACTTCAATTTCA+AGG + chr4_1:36939034-36939053 Msa0545930:CDS 25.0%
! GAGATGTTATAATCTGAATT+TGG + chr4_1:36939130-36939149 Msa0545930:intron 25.0%
! AATCTGAATTTGGTTGTTTA+TGG + chr4_1:36939140-36939159 Msa0545930:intron 25.0%
!!! TTTTGTTTTAGAGGACATAT+TGG + chr4_1:36939208-36939227 Msa0545930:intron 25.0%
!! AGTAAAACTTTCATTTTCCT+TGG - chr4_1:36939500-36939519 None:intergenic 25.0%
!!! AATATGTTTTTCAAGCATGA+TGG + chr4_1:36939652-36939671 Msa0545930:intron 25.0%
!! ATGATTAGTTGATTTCAGTT+AGG - chr4_1:36940144-36940163 None:intergenic 25.0%
!!! CTAACACTCACTTTTTTATT+GGG - chr4_1:36940338-36940357 None:intergenic 25.0%
!!! TCTAACACTCACTTTTTTAT+TGG - chr4_1:36940339-36940358 None:intergenic 25.0%
!!! AATTTTGCTTTTGCATTTCT+CGG + chr4_1:36940478-36940497 Msa0545930:intron 25.0%
!! AGAATCGAAAAACTACATTT+TGG + chr4_1:36940528-36940547 Msa0545930:intron 25.0%
!! AATATCACTTGATAATTGGA+AGG - chr4_1:36941131-36941150 None:intergenic 25.0%
!!! TTATCAAGTGATATTTTGAC+TGG + chr4_1:36941136-36941155 Msa0545930:three_prime_UTR 25.0%
!!! TGATATTTTGACTGGTAAAT+TGG + chr4_1:36941144-36941163 Msa0545930:three_prime_UTR 25.0%
!!! GATATTTTGACTGGTAAATT+GGG + chr4_1:36941145-36941164 Msa0545930:three_prime_UTR 25.0%
!! TTTTTATTGTAACTAGAGGT+AGG + chr4_1:36941232-36941251 Msa0545930:three_prime_UTR 25.0%
AAATGAAGAATGGGACATTT+GGG - chr4_1:36938419-36938438 None:intergenic 30.0%
!! AGTTAATTTTCTGGTATGTG+TGG - chr4_1:36938445-36938464 None:intergenic 30.0%
! AAGGAAATGGAAGAAGATTT+TGG - chr4_1:36938468-36938487 None:intergenic 30.0%
TCAAACTCAACAAAATCAGA+AGG - chr4_1:36939069-36939088 None:intergenic 30.0%
GTTTGATCTTATTCTTGCTA+TGG + chr4_1:36939083-36939102 Msa0545930:CDS 30.0%
!! CCTCTAAAACAAAAACAACA+AGG - chr4_1:36939202-36939221 None:intergenic 30.0%
!!! CCTTGTTGTTTTTGTTTTAG+AGG + chr4_1:36939199-36939218 Msa0545930:intron 30.0%
! TATTGGAAGCTTTTAGTAGA+TGG + chr4_1:36939225-36939244 Msa0545930:exon 30.0%
TCACAGTCAACACAAATATA+AGG - chr4_1:36939606-36939625 None:intergenic 30.0%
!! ATGATTTATTGGTTCACTCT+TGG + chr4_1:36939792-36939811 Msa0545930:intron 30.0%
!!! TCACTCTTGGTTCATTTTTT+TGG + chr4_1:36939805-36939824 Msa0545930:intron 30.0%
!!! TCTTGGTTCATTTTTTTGGA+GGG + chr4_1:36939809-36939828 Msa0545930:intron 30.0%
TGAAATCAACTAATCATAGC+TGG + chr4_1:36940147-36940166 Msa0545930:intron 30.0%
TCATTGGATTGCATATATAC+CGG + chr4_1:36940196-36940215 Msa0545930:intron 30.0%
TTATTGGATGAAATCCATGT+AGG + chr4_1:36940258-36940277 Msa0545930:intron 30.0%
TGGGATATATTTCAAAGTGT+AGG - chr4_1:36940285-36940304 None:intergenic 30.0%
TGGATGTCACTACTTTAAAT+GGG - chr4_1:36940304-36940323 None:intergenic 30.0%
TTTATTGGGTGAAATTCATG+TGG - chr4_1:36940324-36940343 None:intergenic 30.0%
!! ATTTTGCTTTTGCATTTCTC+GGG + chr4_1:36940479-36940498 Msa0545930:intron 30.0%
GATTTGTCATAATGTACATC+AGG + chr4_1:36940604-36940623 Msa0545930:intron 30.0%
!! CTGGCTATTAAAAAGGTATT+AGG - chr4_1:36940825-36940844 None:intergenic 30.0%
! TTTAGGACAATGAAACAACA+AGG - chr4_1:36940906-36940925 None:intergenic 30.0%
TATATAGAAGCCATGGATTT+AGG - chr4_1:36940923-36940942 None:intergenic 30.0%
AATCCATGGCTTCTATATAT+TGG + chr4_1:36940924-36940943 Msa0545930:three_prime_UTR 30.0%
ATCCATGGCTTCTATATATT+GGG + chr4_1:36940925-36940944 Msa0545930:three_prime_UTR 30.0%
TTGTGACTTAATTCTTGGAT+AGG - chr4_1:36940965-36940984 None:intergenic 30.0%
TGGGAATATTTAACATGTTC+AGG + chr4_1:36941164-36941183 Msa0545930:three_prime_UTR 30.0%
GGGAATATTTAACATGTTCA+GGG + chr4_1:36941165-36941184 Msa0545930:three_prime_UTR 30.0%
!!! GGCTTTTTTATTGTAACTAG+AGG + chr4_1:36941228-36941247 Msa0545930:three_prime_UTR 30.0%
ATGAAGACACTATACACTGA+CGG + chr4_1:36938339-36938358 Msa0545930:CDS 35.0%
GAAATGAAGAATGGGACATT+TGG - chr4_1:36938420-36938439 None:intergenic 35.0%
GTGTGGAAGAAATGAAGAAT+GGG - chr4_1:36938428-36938447 None:intergenic 35.0%
TGTGTGGAAGAAATGAAGAA+TGG - chr4_1:36938429-36938448 None:intergenic 35.0%
TTCTCCAATTCAAGCTTCAA+TGG + chr4_1:36938503-36938522 Msa0545930:CDS 35.0%
CATACAAAGAGAACAGAGAA+TGG - chr4_1:36938561-36938580 None:intergenic 35.0%
TCTGTTCTCTTTGTATGTCT+TGG + chr4_1:36938564-36938583 Msa0545930:CDS 35.0%
!! TCACAGACTTGGTGAAAAAA+AGG + chr4_1:36938622-36938641 Msa0545930:CDS 35.0%
!! CACAGACTTGGTGAAAAAAA+GGG + chr4_1:36938623-36938642 Msa0545930:CDS 35.0%
!! ACAGACTTGGTGAAAAAAAG+GGG + chr4_1:36938624-36938643 Msa0545930:CDS 35.0%
! AAGTTCAGAATTGATTCTGC+TGG + chr4_1:36938657-36938676 Msa0545930:CDS 35.0%
! ATTGATTCTGCTGGTACAAT+CGG + chr4_1:36938666-36938685 Msa0545930:CDS 35.0%
TACAATCGGTTATCATGAAG+TGG + chr4_1:36938680-36938699 Msa0545930:intron 35.0%
ACAATCGGTTATCATGAAGT+GGG + chr4_1:36938681-36938700 Msa0545930:intron 35.0%
ACAAAATCAGAAGGCTTGAT+TGG - chr4_1:36939060-36939079 None:intergenic 35.0%
! GAACTTACTCACCTTTTTGT+GGG - chr4_1:36939279-36939298 None:intergenic 35.0%
! TGAACTTACTCACCTTTTTG+TGG - chr4_1:36939280-36939299 None:intergenic 35.0%
TTGTACTAAATCGCGTATTG+TGG + chr4_1:36939408-36939427 Msa0545930:intron 35.0%
CTTGACAACATTGAATTCCA+AGG + chr4_1:36939480-36939499 Msa0545930:intron 35.0%
CAGTCAACACAAATATAAGG+CGG - chr4_1:36939603-36939622 None:intergenic 35.0%
TATACCTTCTCAAAACCTTG+AGG - chr4_1:36939766-36939785 None:intergenic 35.0%
!!! CTCTTGGTTCATTTTTTTGG+AGG + chr4_1:36939808-36939827 Msa0545930:intron 35.0%
CTAGTGAAATAGTTGAGTGT+TGG + chr4_1:36940073-36940092 Msa0545930:intron 35.0%
! TTTTCAACACCCACTCTTAT+TGG + chr4_1:36940242-36940261 Msa0545930:intron 35.0%
GGATTTCATCCAATAAGAGT+GGG - chr4_1:36940254-36940273 None:intergenic 35.0%
TGGATTTCATCCAATAAGAG+TGG - chr4_1:36940255-36940274 None:intergenic 35.0%
GTGGATGTCACTACTTTAAA+TGG - chr4_1:36940305-36940324 None:intergenic 35.0%
! ATCATTGCTGGATAGCATTT+TGG + chr4_1:36940775-36940794 Msa0545930:three_prime_UTR 35.0%
! GGATCTTCTGGCTATTAAAA+AGG - chr4_1:36940832-36940851 None:intergenic 35.0%
GTTTCATTGTCCTAAATCCA+TGG + chr4_1:36940910-36940929 Msa0545930:three_prime_UTR 35.0%
TTCCCAATATATAGAAGCCA+TGG - chr4_1:36940930-36940949 None:intergenic 35.0%
ACTTAATTCTTGGATAGGCA+TGG - chr4_1:36940960-36940979 None:intergenic 35.0%
CGCATTTGTGACTTAATTCT+TGG - chr4_1:36940970-36940989 None:intergenic 35.0%
ACTCAGCAGATTATATAGCA+TGG + chr4_1:36941043-36941062 Msa0545930:three_prime_UTR 35.0%
! ACTTGATAATTGGAAGGAGA+GGG - chr4_1:36941125-36941144 None:intergenic 35.0%
TATTCCTCTGCAACCAATTG+AGG - chr4_1:36938315-36938334 None:intergenic 40.0%
AAATGGGAAGAGTGGCAAAA+TGG - chr4_1:36938384-36938403 None:intergenic 40.0%
! AAGAATGGGACATTTGGGTT+TGG - chr4_1:36938414-36938433 None:intergenic 40.0%
GAAGCCATTGAAGCTTGAAT+TGG - chr4_1:36938510-36938529 None:intergenic 40.0%
!!! TGAAGGTGTTTTCACAGACT+TGG + chr4_1:36938611-36938630 Msa0545930:CDS 40.0%
ATGAAGCTGATCCAAGAATG+AGG + chr4_1:36938989-36939008 Msa0545930:CDS 40.0%
! TTTGAAGCTGACCTCATTCT+TGG - chr4_1:36939003-36939022 None:intergenic 40.0%
GCTATGGACAAGCAAAATAG+AGG + chr4_1:36939099-36939118 Msa0545930:CDS 40.0%
TGGACAAGCAAAATAGAGGT+AGG + chr4_1:36939103-36939122 Msa0545930:intron 40.0%
GGACAAGCAAAATAGAGGTA+GGG + chr4_1:36939104-36939123 Msa0545930:intron 40.0%
GACAAGCAAAATAGAGGTAG+GGG + chr4_1:36939105-36939124 Msa0545930:intron 40.0%
GAATTCAACCCCTTTATCCA+AGG + chr4_1:36939305-36939324 Msa0545930:intron 40.0%
ACAACACTGCCTTGGATAAA+GGG - chr4_1:36939317-36939336 None:intergenic 40.0%
GCGGTTCAAGTCCTTAATTT+AGG - chr4_1:36939584-36939603 None:intergenic 40.0%
! TTCAAGCATGATGGAGAGTT+TGG + chr4_1:36939661-36939680 Msa0545930:intron 40.0%
! TGGAGAGTTTGGAATAATGC+AGG + chr4_1:36939672-36939691 Msa0545930:intron 40.0%
TGACACTGACACATCAACAT+TGG - chr4_1:36940015-36940034 None:intergenic 40.0%
ATTGCATATATACCGGTGTG+AGG + chr4_1:36940203-36940222 Msa0545930:intron 40.0%
ATATATACCGGTGTGAGGAA+TGG + chr4_1:36940208-36940227 Msa0545930:intron 40.0%
TTCAAAGTGTAGGACCTACA+TGG - chr4_1:36940275-36940294 None:intergenic 40.0%
GTTCTTTGCACTAGCAATTC+CGG + chr4_1:36940377-36940396 Msa0545930:intron 40.0%
CAAGTACTAAGTATGTGCAC+CGG - chr4_1:36940399-36940418 None:intergenic 40.0%
AGATGCTTGTGAATCATTGC+TGG + chr4_1:36940763-36940782 Msa0545930:three_prime_UTR 40.0%
ATAGCCAGAAGATCCAATCT+TGG + chr4_1:36940837-36940856 Msa0545930:three_prime_UTR 40.0%
TAGCCAGAAGATCCAATCTT+GGG + chr4_1:36940838-36940857 Msa0545930:three_prime_UTR 40.0%
AATCCCAAGATTGGATCTTC+TGG - chr4_1:36940844-36940863 None:intergenic 40.0%
CTACAGCTTAATCCCAAGAT+TGG - chr4_1:36940853-36940872 None:intergenic 40.0%
TGGGATTAAGCTGTAGAGAT+TGG + chr4_1:36940857-36940876 Msa0545930:three_prime_UTR 40.0%
GATTAAGCTGTAGAGATTGG+CGG + chr4_1:36940860-36940879 Msa0545930:three_prime_UTR 40.0%
TGCTGAGTCTCAGTATTCAA+TGG - chr4_1:36941031-36941050 None:intergenic 40.0%
CTGTTGACATGCACATTACA+AGG + chr4_1:36941083-36941102 Msa0545930:three_prime_UTR 40.0%
! CACTTGATAATTGGAAGGAG+AGG - chr4_1:36941126-36941145 None:intergenic 40.0%
ATCTAGTGGAGCTCCTCAAT+TGG + chr4_1:36938299-36938318 Msa0545930:five_prime_UTR 45.0%
TGGGTTTGGACAGAGGAAAT+GGG - chr4_1:36938400-36938419 None:intergenic 45.0%
TTGGGTTTGGACAGAGGAAA+TGG - chr4_1:36938401-36938420 None:intergenic 45.0%
TGAAAACACCTTCAGCAGCT+GGG - chr4_1:36938605-36938624 None:intergenic 45.0%
AGGTCAGCTTCAAAAAGACG+TGG + chr4_1:36939009-36939028 Msa0545930:CDS 45.0%
TGTGGGCATCTTCAGGTAAA+GGG - chr4_1:36939262-36939281 None:intergenic 45.0%
TTGTGGGCATCTTCAGGTAA+AGG - chr4_1:36939263-36939282 None:intergenic 45.0%
ACCTGAAGATGCCCACAAAA+AGG + chr4_1:36939265-36939284 Msa0545930:three_prime_UTR 45.0%
! ACCTTTTTGTGGGCATCTTC+AGG - chr4_1:36939269-36939288 None:intergenic 45.0%
CAACACTGCCTTGGATAAAG+GGG - chr4_1:36939316-36939335 None:intergenic 45.0%
GACAACACTGCCTTGGATAA+AGG - chr4_1:36939318-36939337 None:intergenic 45.0%
GCTACAACATTGTAGCGTAG+TGG - chr4_1:36939371-36939390 None:intergenic 45.0%
GCATGATGAAACTGAAGTCC+CGG + chr4_1:36939723-36939742 Msa0545930:three_prime_UTR 45.0%
!!! TGGACCTCAAGGTTTTGAGA+AGG + chr4_1:36939759-36939778 Msa0545930:three_prime_UTR 45.0%
CACACTTTCATGAGAAGTGG+CGG - chr4_1:36939926-36939945 None:intergenic 45.0%
GGACACACTTTCATGAGAAG+TGG - chr4_1:36939929-36939948 None:intergenic 45.0%
TGATGTGTCAGTGTCATGTG+TGG + chr4_1:36940019-36940038 Msa0545930:intron 45.0%
GAGCTATGAAAACCCGACAT+GGG - chr4_1:36940047-36940066 None:intergenic 45.0%
TGAGCTATGAAAACCCGACA+TGG - chr4_1:36940048-36940067 None:intergenic 45.0%
CGGTGCACATACTTAGTACT+TGG + chr4_1:36940397-36940416 Msa0545930:intron 45.0%
! GTAGAGATTGGCGGTTTTCA+TGG + chr4_1:36940869-36940888 Msa0545930:three_prime_UTR 45.0%
!!! TTGTTAATTTTTTTTAATTT+CGG + chr4_1:36938800-36938819 Msa0545930:intron 5.0%
AGCTCCTCAATTGGTTGCAG+AGG + chr4_1:36938308-36938327 Msa0545930:five_prime_UTR 50.0%
GACAGAGGAAATGGGAAGAG+TGG - chr4_1:36938392-36938411 None:intergenic 50.0%
! GGACATTTGGGTTTGGACAG+AGG - chr4_1:36938407-36938426 None:intergenic 50.0%
GTGAAAACACCTTCAGCAGC+TGG - chr4_1:36938606-36938625 None:intergenic 50.0%
CAAGGCAGTGTTGTCAATCG+CGG + chr4_1:36939323-36939342 Msa0545930:intron 50.0%
GAAGTCCCGGATCCTTACTA+TGG + chr4_1:36939736-36939755 Msa0545930:three_prime_UTR 50.0%
GTCCACCATAGTAAGGATCC+GGG - chr4_1:36939744-36939763 None:intergenic 50.0%
GGTCCACCATAGTAAGGATC+CGG - chr4_1:36939745-36939764 None:intergenic 50.0%
CCTTGAGGTCCACCATAGTA+AGG - chr4_1:36939751-36939770 None:intergenic 50.0%
!! CCTTACTATGGTGGACCTCA+AGG + chr4_1:36939748-36939767 Msa0545930:three_prime_UTR 50.0%
GTCATGTGTGGTTCCCATGT+CGG + chr4_1:36940031-36940050 Msa0545930:intron 50.0%
TCATGTGTGGTTCCCATGTC+GGG + chr4_1:36940032-36940051 Msa0545930:intron 50.0%
ATACCGGTGTGAGGAATGGT+AGG + chr4_1:36940212-36940231 Msa0545930:intron 50.0%
GTTCCTACCATTCCTCACAC+CGG - chr4_1:36940218-36940237 None:intergenic 50.0%
GATCATCATGTTGCACTCGC+AGG + chr4_1:36940724-36940743 Msa0545930:intron 50.0%
TGTAGAAGCCCAGCTGCTGA+AGG + chr4_1:36938594-36938613 Msa0545930:CDS 55.0%
CGCGATTGACAACACTGCCT+TGG - chr4_1:36939325-36939344 None:intergenic 55.0%
GTCCCGGATCCTTACTATGG+TGG + chr4_1:36939739-36939758 Msa0545930:three_prime_UTR 55.0%
GTCAGTGTCGTGTTGGAGGC+TGG - chr4_1:36939950-36939969 None:intergenic 60.0%
GCGTGTCAGTGTCGTGTTGG+AGG - chr4_1:36939954-36939973 None:intergenic 60.0%
CAACACGACACTGACACGCG+CGG + chr4_1:36939955-36939974 Msa0545930:intron 60.0%
CGCGCGTGTCAGTGTCGTGT+TGG - chr4_1:36939957-36939976 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 36938277 36941310 36938277 ID=Msa0545930;Name=Msa0545930
chr4_1 mRNA 36938277 36941310 36938277 ID=Msa0545930-mRNA-1;Parent=Msa0545930;Name=Msa0545930-mRNA-1;_AED=0.04;_eAED=0.04;_QI=62|0.75|0.8|1|0.5|0.6|5|698|171
chr4_1 exon 36938277 36938698 36938277 ID=Msa0545930-mRNA-1:exon:9346;Parent=Msa0545930-mRNA-1
chr4_1 exon 36938985 36939120 36938985 ID=Msa0545930-mRNA-1:exon:9347;Parent=Msa0545930-mRNA-1
chr4_1 exon 36939221 36939286 36939221 ID=Msa0545930-mRNA-1:exon:9348;Parent=Msa0545930-mRNA-1
chr4_1 exon 36939694 36939780 36939694 ID=Msa0545930-mRNA-1:exon:9349;Parent=Msa0545930-mRNA-1
chr4_1 exon 36940746 36941310 36940746 ID=Msa0545930-mRNA-1:exon:9350;Parent=Msa0545930-mRNA-1
chr4_1 five_prime_UTR 36938277 36938338 36938277 ID=Msa0545930-mRNA-1:five_prime_utr;Parent=Msa0545930-mRNA-1
chr4_1 CDS 36938339 36938698 36938339 ID=Msa0545930-mRNA-1:cds;Parent=Msa0545930-mRNA-1
chr4_1 CDS 36938985 36939120 36938985 ID=Msa0545930-mRNA-1:cds;Parent=Msa0545930-mRNA-1
chr4_1 CDS 36939221 36939240 36939221 ID=Msa0545930-mRNA-1:cds;Parent=Msa0545930-mRNA-1
chr4_1 three_prime_UTR 36939241 36939286 36939241 ID=Msa0545930-mRNA-1:three_prime_utr;Parent=Msa0545930-mRNA-1
chr4_1 three_prime_UTR 36939694 36939780 36939694 ID=Msa0545930-mRNA-1:three_prime_utr;Parent=Msa0545930-mRNA-1
chr4_1 three_prime_UTR 36940746 36941310 36940746 ID=Msa0545930-mRNA-1:three_prime_utr;Parent=Msa0545930-mRNA-1
Gene Sequence

>Msa0545930

ATGAAGACACTATACACTGACGGAACAAACCTTATTAATTTACCATTTTGCCACTCTTCCCATTTCCTCTGTCCAAACCCAAATGTCCCATTCTTCATTTCTTCCACACATACCAGAAAATTAACTCCAAAATCTTCTTCCATTTCCTTCAAAATTAATCACTTTTCTCCAATTCAAGCTTCAATGGCTTCATCAACAGTCACAGAAGACACAAACAAACCATTCTCTGTTCTCTTTGTATGTCTTGGCAACATCTGTAGAAGCCCAGCTGCTGAAGGTGTTTTCACAGACTTGGTGAAAAAAAGGGGTTTTGATTCAAAGTTCAGAATTGATTCTGCTGGTACAATCGGTTATCATGAAGGTAATGAAGCTGATCCAAGAATGAGGTCAGCTTCAAAAAGACGTGGTATTCAGATAACTTCAATTTCAAGGCCAATCAAGCCTTCTGATTTTGTTGAGTTTGATCTTATTCTTGCTATGGACAAGCAAAATAGAGGACATATTGGAAGCTTTTAG
Protein sequence

>Msa0545930

MKTLYTDGTNLINLPFCHSSHFLCPNPNVPFFISSTHTRKLTPKSSSISFKINHFSPIQASMASSTVTEDTNKPFSVLFVCLGNICRSPAAEGVFTDLVKKRGFDSKFRIDSAGTIGYHEGNEADPRMRSASKRRGIQITSISRPIKPSDFVEFDLILAMDKQNRGHIGSF*