AlfalfaGEDB | Msa1124020

M. sativa cultivar zhongmu-4 / Msa1124020

KEGG: -
GO: -
PFAM: PF04689.16

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1124020	XP_013449980.1	100.000	73	0	0	1	73	1	73	8.19e-43	143

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1124020	sp\|Q7XLX6\|S1FA2_ORYSJ	65.000	80	21	1	1	73	1	80	7.37e-15	65.5

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1124020	A0A072U4F5	100.000	73	0	0	1	73	1	73	3.91e-43	143

TFs/TRs:

Gene ID	Type	Classification
Msa1124020	TF	S1Fa-like

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000050	Msa1124020	0.804856	1.767382e-49	-8.615850e-47
Msa0009180	Msa1124020	0.800450	1.433413e-48	-8.615850e-47
Msa0044000	Msa1124020	0.801835	7.464933e-49	-8.615850e-47
Msa0079180	Msa1124020	0.811435	7.012117e-51	-8.615850e-47
Msa0081090	Msa1124020	0.811900	5.555826e-51	-8.615850e-47
Msa0610800	Msa1124020	0.805298	1.428261e-49	-8.615850e-47
Msa0663810	Msa1124020	0.825944	3.555398e-54	-8.615850e-47
Msa0765910	Msa1124020	0.812209	4.756349e-51	-8.615850e-47
Msa0124060	Msa1124020	0.826373	2.811380e-54	-8.615850e-47
Msa0168500	Msa1124020	0.808233	3.424479e-50	-8.615850e-47
Msa0198340	Msa1124020	0.801128	1.042202e-48	-8.615850e-47
Msa0452710	Msa1124020	0.802402	5.706248e-49	-8.615850e-47
Msa0457960	Msa1124020	0.809647	1.706229e-50	-8.615850e-47
Msa0997370	Msa1124020	0.860659	1.770501e-63	-8.615850e-47
Msa1012240	Msa1124020	0.800334	1.513526e-48	-8.615850e-47
Msa1026740	Msa1124020	0.801975	6.986733e-49	-8.615850e-47
Msa1029310	Msa1124020	0.818096	2.342233e-52	-8.615850e-47
Msa1036610	Msa1124020	0.809063	2.276545e-50	-8.615850e-47
Msa1053720	Msa1124020	0.800572	1.353644e-48	-8.615850e-47
Msa1069220	Msa1124020	0.824927	6.189876e-54	-8.615850e-47
Msa1083300	Msa1124020	0.804792	1.822331e-49	-8.615850e-47
Msa0244640	Msa1124020	0.809369	1.957537e-50	-8.615850e-47
Msa0292370	Msa1124020	0.822865	1.884710e-53	-8.615850e-47
Msa0340470	Msa1124020	0.830510	2.819665e-55	-8.615850e-47
Msa1121490	Msa1124020	0.809922	1.489211e-50	-8.615850e-47
Msa1123380	Msa1124020	0.812282	4.584883e-51	-8.615850e-47
Msa1124020	Msa1148090	0.800471	1.419113e-48	-8.615850e-47
Msa1124020	Msa1388510	0.803141	4.016805e-49	-8.615850e-47
Msa0498490	Msa1124020	0.804136	2.496269e-49	-8.615850e-47
Msa0504210	Msa1124020	0.817960	2.514051e-52	-8.615850e-47
Msa0546050	Msa1124020	0.816225	6.170004e-52	-8.615850e-47
Msa0564390	Msa1124020	0.812211	4.752446e-51	-8.615850e-47
Msa0602390	Msa1124020	0.801365	9.317701e-49	-8.615850e-47
Msa0909740	Msa1124020	0.806702	7.236130e-50	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1124020	MtrunA17_Chr7g0263871	100.000	73	0	0	1	73	1	73	7.53e-47	143
Msa1124020	MtrunA17_Chr7g0263831	85.897	78	6	1	1	73	7	84	2.20e-18	71.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1124020	AT2G37120.1	76.190	63	15	0	11	73	14	76	5.53e-15	63.2
Msa1124020	AT3G53370.1	80.000	60	12	0	14	73	17	76	1.77e-12	57.0

Find 29 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATATTTGGGACAGGTTATT+TGG	0.128418	7_3:-75047936	Msa1124020:three_prime_UTR
TACTACTTTAGCAACATATT+TGG	0.141555	7_3:-75047950	Msa1124020:three_prime_UTR
TTAATAGTTCTCCTGGTTAT+TGG	0.281375	7_3:-75048250	Msa1124020:CDS
ACTACTTTAGCAACATATTT+GGG	0.336177	7_3:-75047949	Msa1124020:three_prime_UTR
CGTTTCAAACCTTTCTCTCT+CGG	0.372437	7_3:-75049513	Msa1124020:five_prime_UTR
TGGGGAAATGTTTAGAATGC+TGG	0.422488	7_3:-75048295	Msa1124020:intron
ACCAGGAGAACTATTAAGCC+TGG	0.450176	7_3:+75048256	None:intergenic
CGCGGAGAAGCTTCAAGCTA+TGG	0.466693	7_3:+75049451	None:intergenic
GTTTAGAATGCTGGATCTCA+AGG	0.468079	7_3:-75048286	Msa1124020:intron
GACTTGAATCCGAGAGAGAA+AGG	0.468305	7_3:+75049504	None:intergenic
CCCAGGCTTAATAGTTCTCC+TGG	0.470539	7_3:-75048257	Msa1124020:CDS
ATAGTTCTCCTGGTTATTGG+TGG	0.478741	7_3:-75048247	Msa1124020:CDS
TAGTTCTCCTGGTTATTGGT+GGG	0.495632	7_3:-75048246	Msa1124020:CDS
TTGCTGTTGACATTTCTCAT+TGG	0.497549	7_3:-75048223	Msa1124020:CDS
TTTAGCAACATATTTGGGAC+AGG	0.518479	7_3:-75047944	Msa1124020:three_prime_UTR
AAGCAAGGAGTCTCTGCACC+TGG	0.518649	7_3:-75048109	Msa1124020:CDS
AAGCTTCTCCGCGTCAACCA+TGG	0.519128	7_3:-75049441	Msa1124020:exon
ATACAGTGCCTCTACTCTCC+AGG	0.531169	7_3:+75048091	None:intergenic
AATGAGCTAATGTAAGAAAG+AGG	0.535627	7_3:+75047887	None:intergenic
ATTTGGGACAGGTTATTTGG+TGG	0.553725	7_3:-75047933	Msa1124020:three_prime_UTR
GCTCTACGTTTATGCACAGA+AGG	0.594297	7_3:-75048191	Msa1124020:CDS
CCAGGAGAACTATTAAGCCT+GGG	0.597197	7_3:+75048257	None:intergenic
ACAGCAACCCACCAATAACC+AGG	0.598951	7_3:+75048239	None:intergenic
TCTGTCAATAAAACACAGGA+TGG	0.607884	7_3:+75047991	None:intergenic
GGATCTCAAGGGTTCAACCC+AGG	0.613072	7_3:-75048274	Msa1124020:CDS
TTTAGAATGCTGGATCTCAA+GGG	0.619147	7_3:-75048285	Msa1124020:intron
TTTATCGGCCATGGTTGACG+CGG	0.638815	7_3:+75049433	None:intergenic
TCTCTGCACCTGGAGAGTAG+AGG	0.653251	7_3:-75048099	Msa1124020:exon
ACTTTCTGTCAATAAAACAC+AGG	0.663804	7_3:+75047987	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTTTTTTTATTCACATTT+AGG	-	chr7_3:75048232-75048251	Msa1124020:CDS	10.0%
!!	AAAAAAAATAAAATTTCCAA+AGG	+	chr7_3:75049008-75049027	None:intergenic	10.0%
!!!	TTTTTTTTTTATTCACATTT+AGG	-	chr7_3:75048232-75048251	Msa1124020:CDS	10.0%
!!	AAAAAAAATAAAATTTCCAA+AGG	+	chr7_3:75049008-75049027	None:intergenic	10.0%
!!	AAATAAATAATGAAGAATGT+GGG	+	chr7_3:75048037-75048056	None:intergenic	15.0%
!!	AAAATAAATAATGAAGAATG+TGG	+	chr7_3:75048038-75048057	None:intergenic	15.0%
!!	GATCTATTCATAATTAAATT+AGG	-	chr7_3:75048208-75048227	Msa1124020:CDS	15.0%
!!	TTATTTGAGAATAACAATAA+GGG	+	chr7_3:75048599-75048618	None:intergenic	15.0%
!!	CATATTATTATTTGTATACA+TGG	-	chr7_3:75048940-75048959	Msa1124020:intron	15.0%
!!	AAATAAATAATGAAGAATGT+GGG	+	chr7_3:75048037-75048056	None:intergenic	15.0%
!!	AAAATAAATAATGAAGAATG+TGG	+	chr7_3:75048038-75048057	None:intergenic	15.0%
!!	GATCTATTCATAATTAAATT+AGG	-	chr7_3:75048208-75048227	Msa1124020:CDS	15.0%
!!	TTATTTGAGAATAACAATAA+GGG	+	chr7_3:75048599-75048618	None:intergenic	15.0%
!!	CATATTATTATTTGTATACA+TGG	-	chr7_3:75048940-75048959	Msa1124020:intron	15.0%
!!	TGACAAAGAGAAAAAATAAA+CGG	+	chr7_3:75047912-75047931	None:intergenic	20.0%
!!	TTTCAGATCTTAAAAATTCA+TGG	-	chr7_3:75048057-75048076	Msa1124020:three_prime_UTR	20.0%
!!!	TTTTATTCACATTTAGGTTA+AGG	-	chr7_3:75048238-75048257	Msa1124020:CDS	20.0%
!!	CTTATTTGAGAATAACAATA+AGG	+	chr7_3:75048600-75048619	None:intergenic	20.0%
!!	AGATTCTACTTTGAATATAA+TGG	-	chr7_3:75048822-75048841	Msa1124020:intron	20.0%
!!	GATTCTACTTTGAATATAAT+GGG	-	chr7_3:75048823-75048842	Msa1124020:intron	20.0%
!!	TTATTATTTGTATACATGGA+TGG	-	chr7_3:75048944-75048963	Msa1124020:intron	20.0%
!!!	CAAAAAAAAGCACTTAAATA+AGG	+	chr7_3:75049067-75049086	None:intergenic	20.0%
!!!	ATTACCTTGTACTTTTTAAA+TGG	-	chr7_3:75049343-75049362	Msa1124020:intron	20.0%
!!	TGACAAAGAGAAAAAATAAA+CGG	+	chr7_3:75047912-75047931	None:intergenic	20.0%
!!	TTTCAGATCTTAAAAATTCA+TGG	-	chr7_3:75048057-75048076	Msa1124020:three_prime_UTR	20.0%
!!!	TTTTATTCACATTTAGGTTA+AGG	-	chr7_3:75048238-75048257	Msa1124020:CDS	20.0%
!!	CTTATTTGAGAATAACAATA+AGG	+	chr7_3:75048600-75048619	None:intergenic	20.0%
!!	AGATTCTACTTTGAATATAA+TGG	-	chr7_3:75048822-75048841	Msa1124020:intron	20.0%
!!	GATTCTACTTTGAATATAAT+GGG	-	chr7_3:75048823-75048842	Msa1124020:intron	20.0%
!!	TTATTATTTGTATACATGGA+TGG	-	chr7_3:75048944-75048963	Msa1124020:intron	20.0%
!!!	CAAAAAAAAGCACTTAAATA+AGG	+	chr7_3:75049067-75049086	None:intergenic	20.0%
!!!	ATTACCTTGTACTTTTTAAA+TGG	-	chr7_3:75049343-75049362	Msa1124020:intron	20.0%
!!!	GGAATTGATTTGAAGAAAAT+CGG	+	chr7_3:75048016-75048035	None:intergenic	25.0%
!!!	TGCTTTATTAGACACTTTTA+AGG	-	chr7_3:75048311-75048330	Msa1124020:intron	25.0%
!	AAAATGCATATGATAAAACC+TGG	+	chr7_3:75048555-75048574	None:intergenic	25.0%
!	GGTTACAAATAACATCAATA+TGG	+	chr7_3:75048578-75048597	None:intergenic	25.0%
!!!	TTAATTTGAGAGTGATTTTG+TGG	+	chr7_3:75048761-75048780	None:intergenic	25.0%
!!	TTTACATGTCAATTTTCCTT+TGG	-	chr7_3:75048989-75049008	Msa1124020:intron	25.0%
!	CTCAAAAAAGAAGATGAAAA+AGG	-	chr7_3:75049249-75049268	Msa1124020:intron	25.0%
!!!	TTCTAATGTTTTGTTGAATG+TGG	-	chr7_3:75049320-75049339	Msa1124020:intron	25.0%
!!	AGAACCATTTAAAAAGTACA+AGG	+	chr7_3:75049350-75049369	None:intergenic	25.0%
!	TACTACTTTAGCAACATATT+TGG	-	chr7_3:75049439-75049458	Msa1124020:exon	25.0%
!	ACTACTTTAGCAACATATTT+GGG	-	chr7_3:75049440-75049459	Msa1124020:exon	25.0%
!!!	GGAATTGATTTGAAGAAAAT+CGG	+	chr7_3:75048016-75048035	None:intergenic	25.0%
!!!	TGCTTTATTAGACACTTTTA+AGG	-	chr7_3:75048311-75048330	Msa1124020:intron	25.0%
!	AAAATGCATATGATAAAACC+TGG	+	chr7_3:75048555-75048574	None:intergenic	25.0%
!	GGTTACAAATAACATCAATA+TGG	+	chr7_3:75048578-75048597	None:intergenic	25.0%
!!!	TTAATTTGAGAGTGATTTTG+TGG	+	chr7_3:75048761-75048780	None:intergenic	25.0%
!!	TTTACATGTCAATTTTCCTT+TGG	-	chr7_3:75048989-75049008	Msa1124020:intron	25.0%
!	CTCAAAAAAGAAGATGAAAA+AGG	-	chr7_3:75049249-75049268	Msa1124020:intron	25.0%
!!!	TTCTAATGTTTTGTTGAATG+TGG	-	chr7_3:75049320-75049339	Msa1124020:intron	25.0%
!!	AGAACCATTTAAAAAGTACA+AGG	+	chr7_3:75049350-75049369	None:intergenic	25.0%
!	TACTACTTTAGCAACATATT+TGG	-	chr7_3:75049439-75049458	Msa1124020:exon	25.0%
!	ACTACTTTAGCAACATATTT+GGG	-	chr7_3:75049440-75049459	Msa1124020:exon	25.0%
!!!	TAATTTTTTACCTCCATCAC+TGG	-	chr7_3:75048156-75048175	Msa1124020:CDS	30.0%
!!!	AATTTTTTACCTCCATCACT+GGG	-	chr7_3:75048157-75048176	Msa1124020:CDS	30.0%
	TATGAATAGATCCACAAGTA+AGG	+	chr7_3:75048200-75048219	None:intergenic	30.0%
!	TTAGATCATGATGATGATGA+TGG	-	chr7_3:75048421-75048440	Msa1124020:intron	30.0%
	AACCATTCAACACAAATTGA+TGG	+	chr7_3:75048530-75048549	None:intergenic	30.0%
	ATTCTCAAATAAGTCTGTGT+AGG	-	chr7_3:75048607-75048626	Msa1124020:intron	30.0%
!	TTTTGTGGAGACATACAAAA+GGG	+	chr7_3:75048746-75048765	None:intergenic	30.0%
!	ATTTTGTGGAGACATACAAA+AGG	+	chr7_3:75048747-75048766	None:intergenic	30.0%
	ATTGCAGAGCAAATAAAGAA+TGG	+	chr7_3:75048919-75048938	None:intergenic	30.0%
!!!	TGCTTTTTTTTGTTGCTCTT+GGG	-	chr7_3:75049075-75049094	Msa1124020:intron	30.0%
	TTAATAGTTCTCCTGGTTAT+TGG	-	chr7_3:75049139-75049158	Msa1124020:intron	30.0%
!!!	TTCATCTTCTTTTTTGAGAC+TGG	+	chr7_3:75049247-75049266	None:intergenic	30.0%
	AAAAGAAGATGAAAAAGGAG+AGG	-	chr7_3:75049254-75049273	Msa1124020:intron	30.0%
!!	GTACTTTTTAAATGGTTCTG+CGG	-	chr7_3:75049351-75049370	Msa1124020:intron	30.0%
	ACTTTCTGTCAATAAAACAC+AGG	+	chr7_3:75049405-75049424	None:intergenic	30.0%
	AATGAGCTAATGTAAGAAAG+AGG	+	chr7_3:75049505-75049524	None:intergenic	30.0%
!!!	TAATTTTTTACCTCCATCAC+TGG	-	chr7_3:75048156-75048175	Msa1124020:CDS	30.0%
!!!	AATTTTTTACCTCCATCACT+GGG	-	chr7_3:75048157-75048176	Msa1124020:CDS	30.0%
	TATGAATAGATCCACAAGTA+AGG	+	chr7_3:75048200-75048219	None:intergenic	30.0%
!	TTAGATCATGATGATGATGA+TGG	-	chr7_3:75048421-75048440	Msa1124020:intron	30.0%
	AACCATTCAACACAAATTGA+TGG	+	chr7_3:75048530-75048549	None:intergenic	30.0%
	ATTCTCAAATAAGTCTGTGT+AGG	-	chr7_3:75048607-75048626	Msa1124020:intron	30.0%
!	TTTTGTGGAGACATACAAAA+GGG	+	chr7_3:75048746-75048765	None:intergenic	30.0%
!	ATTTTGTGGAGACATACAAA+AGG	+	chr7_3:75048747-75048766	None:intergenic	30.0%
	ATTGCAGAGCAAATAAAGAA+TGG	+	chr7_3:75048919-75048938	None:intergenic	30.0%
!!!	TGCTTTTTTTTGTTGCTCTT+GGG	-	chr7_3:75049075-75049094	Msa1124020:intron	30.0%
	TTAATAGTTCTCCTGGTTAT+TGG	-	chr7_3:75049139-75049158	Msa1124020:intron	30.0%
!!!	TTCATCTTCTTTTTTGAGAC+TGG	+	chr7_3:75049247-75049266	None:intergenic	30.0%
	AAAAGAAGATGAAAAAGGAG+AGG	-	chr7_3:75049254-75049273	Msa1124020:intron	30.0%
!!	GTACTTTTTAAATGGTTCTG+CGG	-	chr7_3:75049351-75049370	Msa1124020:intron	30.0%
	ACTTTCTGTCAATAAAACAC+AGG	+	chr7_3:75049405-75049424	None:intergenic	30.0%
	AATGAGCTAATGTAAGAAAG+AGG	+	chr7_3:75049505-75049524	None:intergenic	30.0%
	AGAAAATCGGATCTAAGAAG+AGG	+	chr7_3:75048003-75048022	None:intergenic	35.0%
	AGGGCATAGAATATGAGAAA+GGG	+	chr7_3:75048099-75048118	None:intergenic	35.0%
!	AAGGGCATAGAATATGAGAA+AGG	+	chr7_3:75048100-75048119	None:intergenic	35.0%
!	TGAAAGTATGAACGAGCAAA+GGG	+	chr7_3:75048118-75048137	None:intergenic	35.0%
!	TTGAAAGTATGAACGAGCAA+AGG	+	chr7_3:75048119-75048138	None:intergenic	35.0%
	ATAACTGAAAAACCCAGTGA+TGG	+	chr7_3:75048172-75048191	None:intergenic	35.0%
	AGTTATATACGCCTTACTTG+TGG	-	chr7_3:75048186-75048205	Msa1124020:CDS	35.0%
!	TCAACACAAATTGATGGCAT+TGG	+	chr7_3:75048524-75048543	None:intergenic	35.0%
	TGCCATCAATTTGTGTTGAA+TGG	-	chr7_3:75048525-75048544	Msa1124020:intron	35.0%
!	TTTGTGTTGAATGGTTTTCC+AGG	-	chr7_3:75048534-75048553	Msa1124020:intron	35.0%
	ATTGTATTGTACACGCGTTA+AGG	-	chr7_3:75048647-75048666	Msa1124020:intron	35.0%
!	AAGTAGAATCTCTGATTTGC+AGG	+	chr7_3:75048813-75048832	None:intergenic	35.0%
!	TTTGAAACAAAGGAGAGAAG+TGG	+	chr7_3:75048854-75048873	None:intergenic	35.0%
!!	CCAGAGATTCTTTGAAACAA+AGG	+	chr7_3:75048864-75048883	None:intergenic	35.0%
!	CCTTTGTTTCAAAGAATCTC+TGG	-	chr7_3:75048861-75048880	Msa1124020:intron	35.0%
!!!	GTGCTTTTTTTTGTTGCTCT+TGG	-	chr7_3:75049074-75049093	Msa1124020:intron	35.0%
!!!	GCTTTTTTTTGTTGCTCTTG+GGG	-	chr7_3:75049076-75049095	Msa1124020:intron	35.0%
!	TTTAGAATGCTGGATCTCAA+GGG	-	chr7_3:75049104-75049123	Msa1124020:intron	35.0%
!	TTGCTGTTGACATTTCTCAT+TGG	-	chr7_3:75049166-75049185	Msa1124020:intron	35.0%
	TCTGTCAATAAAACACAGGA+TGG	+	chr7_3:75049401-75049420	None:intergenic	35.0%
!	TTTAGCAACATATTTGGGAC+AGG	-	chr7_3:75049445-75049464	Msa1124020:five_prime_UTR	35.0%
!	CATATTTGGGACAGGTTATT+TGG	-	chr7_3:75049453-75049472	Msa1124020:five_prime_UTR	35.0%
	AGAAAATCGGATCTAAGAAG+AGG	+	chr7_3:75048003-75048022	None:intergenic	35.0%
	AGGGCATAGAATATGAGAAA+GGG	+	chr7_3:75048099-75048118	None:intergenic	35.0%
!	AAGGGCATAGAATATGAGAA+AGG	+	chr7_3:75048100-75048119	None:intergenic	35.0%
!	TGAAAGTATGAACGAGCAAA+GGG	+	chr7_3:75048118-75048137	None:intergenic	35.0%
!	TTGAAAGTATGAACGAGCAA+AGG	+	chr7_3:75048119-75048138	None:intergenic	35.0%
	ATAACTGAAAAACCCAGTGA+TGG	+	chr7_3:75048172-75048191	None:intergenic	35.0%
	AGTTATATACGCCTTACTTG+TGG	-	chr7_3:75048186-75048205	Msa1124020:CDS	35.0%
!	TCAACACAAATTGATGGCAT+TGG	+	chr7_3:75048524-75048543	None:intergenic	35.0%
	TGCCATCAATTTGTGTTGAA+TGG	-	chr7_3:75048525-75048544	Msa1124020:intron	35.0%
!	TTTGTGTTGAATGGTTTTCC+AGG	-	chr7_3:75048534-75048553	Msa1124020:intron	35.0%
	ATTGTATTGTACACGCGTTA+AGG	-	chr7_3:75048647-75048666	Msa1124020:intron	35.0%
!	AAGTAGAATCTCTGATTTGC+AGG	+	chr7_3:75048813-75048832	None:intergenic	35.0%
!	TTTGAAACAAAGGAGAGAAG+TGG	+	chr7_3:75048854-75048873	None:intergenic	35.0%
!!	CCAGAGATTCTTTGAAACAA+AGG	+	chr7_3:75048864-75048883	None:intergenic	35.0%
!	CCTTTGTTTCAAAGAATCTC+TGG	-	chr7_3:75048861-75048880	Msa1124020:intron	35.0%
!!!	GTGCTTTTTTTTGTTGCTCT+TGG	-	chr7_3:75049074-75049093	Msa1124020:intron	35.0%
!!!	GCTTTTTTTTGTTGCTCTTG+GGG	-	chr7_3:75049076-75049095	Msa1124020:intron	35.0%
!	TTTAGAATGCTGGATCTCAA+GGG	-	chr7_3:75049104-75049123	Msa1124020:intron	35.0%
!	TTGCTGTTGACATTTCTCAT+TGG	-	chr7_3:75049166-75049185	Msa1124020:intron	35.0%
	TCTGTCAATAAAACACAGGA+TGG	+	chr7_3:75049401-75049420	None:intergenic	35.0%
!	TTTAGCAACATATTTGGGAC+AGG	-	chr7_3:75049445-75049464	Msa1124020:five_prime_UTR	35.0%
!	CATATTTGGGACAGGTTATT+TGG	-	chr7_3:75049453-75049472	Msa1124020:five_prime_UTR	35.0%
	CGTTTCAAACCTTTCTCTCT+CGG	-	chr7_3:75047876-75047895	Msa1124020:three_prime_UTR	40.0%
	AGAAGAGGAGAGAAGAATAC+CGG	+	chr7_3:75047988-75048007	None:intergenic	40.0%
	GGGCATAGAATATGAGAAAG+GGG	+	chr7_3:75048098-75048117	None:intergenic	40.0%
!	ATGATGGTGATACTGCAGAA+TGG	-	chr7_3:75048437-75048456	Msa1124020:intron	40.0%
	GGGACTGTGAATCATAAATC+AGG	+	chr7_3:75048726-75048745	None:intergenic	40.0%
	TATACATGGATGGTTGTGAG+TGG	-	chr7_3:75048954-75048973	Msa1124020:intron	40.0%
!	TGGGGAAATGTTTAGAATGC+TGG	-	chr7_3:75049094-75049113	Msa1124020:intron	40.0%
!	GTTTAGAATGCTGGATCTCA+AGG	-	chr7_3:75049103-75049122	Msa1124020:intron	40.0%
	ATAGTTCTCCTGGTTATTGG+TGG	-	chr7_3:75049142-75049161	Msa1124020:intron	40.0%
	TAGTTCTCCTGGTTATTGGT+GGG	-	chr7_3:75049143-75049162	Msa1124020:intron	40.0%
!!!	CTTCTTTTTTCTAGGAGGGA+GGG	+	chr7_3:75049224-75049243	None:intergenic	40.0%
!!!	CTGGCTTCTTTTTTCTAGGA+GGG	+	chr7_3:75049228-75049247	None:intergenic	40.0%
!!!	ACTGGCTTCTTTTTTCTAGG+AGG	+	chr7_3:75049229-75049248	None:intergenic	40.0%
!!!	GAGACTGGCTTCTTTTTTCT+AGG	+	chr7_3:75049232-75049251	None:intergenic	40.0%
!	ATTTGGGACAGGTTATTTGG+TGG	-	chr7_3:75049456-75049475	Msa1124020:five_prime_UTR	40.0%
	CGTTTCAAACCTTTCTCTCT+CGG	-	chr7_3:75047876-75047895	Msa1124020:three_prime_UTR	40.0%
	AGAAGAGGAGAGAAGAATAC+CGG	+	chr7_3:75047988-75048007	None:intergenic	40.0%
	GGGCATAGAATATGAGAAAG+GGG	+	chr7_3:75048098-75048117	None:intergenic	40.0%
!	ATGATGGTGATACTGCAGAA+TGG	-	chr7_3:75048437-75048456	Msa1124020:intron	40.0%
	GGGACTGTGAATCATAAATC+AGG	+	chr7_3:75048726-75048745	None:intergenic	40.0%
	TATACATGGATGGTTGTGAG+TGG	-	chr7_3:75048954-75048973	Msa1124020:intron	40.0%
!	TGGGGAAATGTTTAGAATGC+TGG	-	chr7_3:75049094-75049113	Msa1124020:intron	40.0%
!	GTTTAGAATGCTGGATCTCA+AGG	-	chr7_3:75049103-75049122	Msa1124020:intron	40.0%
	ATAGTTCTCCTGGTTATTGG+TGG	-	chr7_3:75049142-75049161	Msa1124020:intron	40.0%
	TAGTTCTCCTGGTTATTGGT+GGG	-	chr7_3:75049143-75049162	Msa1124020:intron	40.0%
!!!	CTTCTTTTTTCTAGGAGGGA+GGG	+	chr7_3:75049224-75049243	None:intergenic	40.0%
!!!	CTGGCTTCTTTTTTCTAGGA+GGG	+	chr7_3:75049228-75049247	None:intergenic	40.0%
!!!	ACTGGCTTCTTTTTTCTAGG+AGG	+	chr7_3:75049229-75049248	None:intergenic	40.0%
!!!	GAGACTGGCTTCTTTTTTCT+AGG	+	chr7_3:75049232-75049251	None:intergenic	40.0%
!	ATTTGGGACAGGTTATTTGG+TGG	-	chr7_3:75049456-75049475	Msa1124020:five_prime_UTR	40.0%
	GACTTGAATCCGAGAGAGAA+AGG	+	chr7_3:75047888-75047907	None:intergenic	45.0%
	AAGAGGAGAGAAGAATACCG+GGG	+	chr7_3:75047986-75048005	None:intergenic	45.0%
	GAAGAGGAGAGAAGAATACC+GGG	+	chr7_3:75047987-75048006	None:intergenic	45.0%
!	TATGAGAAAGGGGCATGCAA+GGG	+	chr7_3:75048088-75048107	None:intergenic	45.0%
!	ATATGAGAAAGGGGCATGCA+AGG	+	chr7_3:75048089-75048108	None:intergenic	45.0%
	ACTGAAAAACCCAGTGATGG+AGG	+	chr7_3:75048169-75048188	None:intergenic	45.0%
	CCAGGAGAACTATTAAGCCT+GGG	+	chr7_3:75049135-75049154	None:intergenic	45.0%
	ACCAGGAGAACTATTAAGCC+TGG	+	chr7_3:75049136-75049155	None:intergenic	45.0%
	GCTCTACGTTTATGCACAGA+AGG	-	chr7_3:75049198-75049217	Msa1124020:intron	45.0%
!!!	GCTTCTTTTTTCTAGGAGGG+AGG	+	chr7_3:75049225-75049244	None:intergenic	45.0%
	AAAAAGGAGAGGCTGAAGCA+AGG	-	chr7_3:75049265-75049284	Msa1124020:intron	45.0%
	GACTTGAATCCGAGAGAGAA+AGG	+	chr7_3:75047888-75047907	None:intergenic	45.0%
	AAGAGGAGAGAAGAATACCG+GGG	+	chr7_3:75047986-75048005	None:intergenic	45.0%
	GAAGAGGAGAGAAGAATACC+GGG	+	chr7_3:75047987-75048006	None:intergenic	45.0%
!	TATGAGAAAGGGGCATGCAA+GGG	+	chr7_3:75048088-75048107	None:intergenic	45.0%
!	ATATGAGAAAGGGGCATGCA+AGG	+	chr7_3:75048089-75048108	None:intergenic	45.0%
	ACTGAAAAACCCAGTGATGG+AGG	+	chr7_3:75048169-75048188	None:intergenic	45.0%
	CCAGGAGAACTATTAAGCCT+GGG	+	chr7_3:75049135-75049154	None:intergenic	45.0%
	ACCAGGAGAACTATTAAGCC+TGG	+	chr7_3:75049136-75049155	None:intergenic	45.0%
	GCTCTACGTTTATGCACAGA+AGG	-	chr7_3:75049198-75049217	Msa1124020:intron	45.0%
!!!	GCTTCTTTTTTCTAGGAGGG+AGG	+	chr7_3:75049225-75049244	None:intergenic	45.0%
	AAAAAGGAGAGGCTGAAGCA+AGG	-	chr7_3:75049265-75049284	Msa1124020:intron	45.0%
	TTTATCGGCCATGGTTGACG+CGG	+	chr7_3:75047959-75047978	None:intergenic	50.0%
	GAATACCGGGGGGACTTTAT+CGG	+	chr7_3:75047974-75047993	None:intergenic	50.0%
	AGAGGAGAGAAGAATACCGG+GGG	+	chr7_3:75047985-75048004	None:intergenic	50.0%
	ACATGGATGGTTGTGAGTGG+TGG	-	chr7_3:75048957-75048976	Msa1124020:intron	50.0%
	CCCAGGCTTAATAGTTCTCC+TGG	-	chr7_3:75049132-75049151	Msa1124020:intron	50.0%
	ACAGCAACCCACCAATAACC+AGG	+	chr7_3:75049153-75049172	None:intergenic	50.0%
	ATACAGTGCCTCTACTCTCC+AGG	+	chr7_3:75049301-75049320	None:intergenic	50.0%
	TTTATCGGCCATGGTTGACG+CGG	+	chr7_3:75047959-75047978	None:intergenic	50.0%
	GAATACCGGGGGGACTTTAT+CGG	+	chr7_3:75047974-75047993	None:intergenic	50.0%
	AGAGGAGAGAAGAATACCGG+GGG	+	chr7_3:75047985-75048004	None:intergenic	50.0%
	ACATGGATGGTTGTGAGTGG+TGG	-	chr7_3:75048957-75048976	Msa1124020:intron	50.0%
	CCCAGGCTTAATAGTTCTCC+TGG	-	chr7_3:75049132-75049151	Msa1124020:intron	50.0%
	ACAGCAACCCACCAATAACC+AGG	+	chr7_3:75049153-75049172	None:intergenic	50.0%
	ATACAGTGCCTCTACTCTCC+AGG	+	chr7_3:75049301-75049320	None:intergenic	50.0%
	CGCGGAGAAGCTTCAAGCTA+TGG	+	chr7_3:75047941-75047960	None:intergenic	55.0%
	AAGCTTCTCCGCGTCAACCA+TGG	-	chr7_3:75047948-75047967	Msa1124020:three_prime_UTR	55.0%
	GAGGAGAGAAGAATACCGGG+GGG	+	chr7_3:75047984-75048003	None:intergenic	55.0%
	GGATCTCAAGGGTTCAACCC+AGG	-	chr7_3:75049115-75049134	Msa1124020:intron	55.0%
	AAGCAAGGAGTCTCTGCACC+TGG	-	chr7_3:75049280-75049299	Msa1124020:intron	55.0%
	TCTCTGCACCTGGAGAGTAG+AGG	-	chr7_3:75049290-75049309	Msa1124020:intron	55.0%
	CGCGGAGAAGCTTCAAGCTA+TGG	+	chr7_3:75047941-75047960	None:intergenic	55.0%
	AAGCTTCTCCGCGTCAACCA+TGG	-	chr7_3:75047948-75047967	Msa1124020:three_prime_UTR	55.0%
	GAGGAGAGAAGAATACCGGG+GGG	+	chr7_3:75047984-75048003	None:intergenic	55.0%
	GGATCTCAAGGGTTCAACCC+AGG	-	chr7_3:75049115-75049134	Msa1124020:intron	55.0%
	AAGCAAGGAGTCTCTGCACC+TGG	-	chr7_3:75049280-75049299	Msa1124020:intron	55.0%
	TCTCTGCACCTGGAGAGTAG+AGG	-	chr7_3:75049290-75049309	Msa1124020:intron	55.0%
	CATGGCCGATAAAGTCCCCC+CGG	-	chr7_3:75047966-75047985	Msa1124020:three_prime_UTR	60.0%
	CATGGCCGATAAAGTCCCCC+CGG	-	chr7_3:75047966-75047985	Msa1124020:three_prime_UTR	60.0%
	CGGGGGGACTTTATCGGCCA+TGG	+	chr7_3:75047968-75047987	None:intergenic	65.0%
	CGGGGGGACTTTATCGGCCA+TGG	+	chr7_3:75047968-75047987	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr7_3	gene	75047854	75049557	75047854	ID=Msa1124020;Name=Msa1124020
chr7_3	mRNA	75047854	75049557	75047854	ID=Msa1124020-mRNA-1;Parent=Msa1124020;Name=Msa1124020-mRNA-1;_AED=0.00;_eAED=0.00;_QI=113\|1\|1\|1\|1\|1\|2\|248\|73
chr7_3	exon	75047854	75048302	75047854	ID=Msa1124020-mRNA-1:exon:20141;Parent=Msa1124020-mRNA-1
chr7_3	exon	75049424	75049557	75049424	ID=Msa1124020-mRNA-1:exon:20140;Parent=Msa1124020-mRNA-1
chr7_3	five_prime_UTR	75049445	75049557	75049445	ID=Msa1124020-mRNA-1:five_prime_utr;Parent=Msa1124020-mRNA-1
chr7_3	CDS	75049424	75049444	75049424	ID=Msa1124020-mRNA-1:cds;Parent=Msa1124020-mRNA-1
chr7_3	CDS	75048102	75048302	75048102	ID=Msa1124020-mRNA-1:cds;Parent=Msa1124020-mRNA-1
chr7_3	three_prime_UTR	75047854	75048101	75047854	ID=Msa1124020-mRNA-1:three_prime_utr;Parent=Msa1124020-mRNA-1

Gene Sequence	>Msa1124020 ATGGCCGATAAAGTCCCCCCGAATGCTGGATCTCAAGGGTTCAACCCAGGCTTAATAGTTCTCCTGGTTATTGGTGGGTTGCTGTTGACATTTCTCATTGGAAATTATGCGCTCTACGTTTATGCACAGAAGGCCCTCCCTCCTAGAAAAAAGAAGCCAGTCTCAAAAAAGAAGATGAAAAAGGAGAGGCTGAAGCAAGGAGTCTCTGCACCTGGAGAGTAG
Protein sequence	>Msa1124020 MADKVPPNAGSQGFNPGLIVLLVIGGLLLTFLIGNYALYVYAQKALPPRKKKPVSKKKMKKERLKQGVSAPGE*