AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0548130

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0548130 MtrunA17_Chr4g0031301 99.384 649 4 0 1 649 1 649 0.0 1325
Msa0548130 MtrunA17_Chr4g0031321 99.230 649 5 0 1 649 1 649 0.0 1325
Msa0548130 MtrunA17_Chr7g0223291 92.934 651 42 3 1 649 1 649 0.0 1239
Msa0548130 MtrunA17_Chr7g0262821 92.296 649 50 0 1 649 1 649 0.0 1238
Msa0548130 MtrunA17_Chr7g0223181 92.627 651 43 2 1 649 1 648 0.0 1230
Msa0548130 MtrunA17_Chr4g0073741 87.768 654 74 3 1 649 1 653 0.0 1161
Msa0548130 MtrunA17_Chr6g0462681 66.992 615 202 1 1 615 1 614 0.0 850
Msa0548130 MtrunA17_Chr2g0284681 68.046 604 173 3 9 606 8 597 0.0 839
Msa0548130 MtrunA17_Chr7g0223751 66.345 621 204 4 1 619 1 618 0.0 838
Msa0548130 MtrunA17_Chr6g0462601 65.909 616 209 1 1 616 1 615 0.0 834
Msa0548130 MtrunA17_Chr7g0223711 65.056 621 213 3 1 619 1 619 0.0 828
Msa0548130 MtrunA17_Chr7g0221661 65.366 615 209 3 1 613 1 613 0.0 817
Msa0548130 MtrunA17_Chr2g0298851 65.590 619 206 6 1 615 1 616 0.0 813
Msa0548130 MtrunA17_Chr5g0427901 64.172 628 210 4 1 615 1 626 0.0 812
Msa0548130 MtrunA17_Chr7g0223741 66.164 597 198 3 1 595 1 595 0.0 812
Msa0548130 MtrunA17_Chr8g0350731 64.480 625 217 4 1 623 29 650 0.0 807
Msa0548130 MtrunA17_Chr2g0293581 64.927 613 211 3 6 615 7 618 0.0 804
Msa0548130 MtrunA17_Chr8g0388761 65.635 614 205 4 7 618 36 645 0.0 804
Msa0548130 MtrunA17_Chr8g0388671 65.635 614 205 4 7 618 36 645 0.0 802
Msa0548130 MtrunA17_Chr7g0223801 63.371 617 220 5 1 615 1 613 0.0 800
Msa0548130 MtrunA17_Chr7g0223731 64.803 608 209 4 1 606 1 605 0.0 798
Msa0548130 MtrunA17_Chr7g0233311 63.430 618 222 3 1 615 1 617 0.0 798
Msa0548130 MtrunA17_Chr7g0223621 64.098 610 214 4 1 608 1 607 0.0 791
Msa0548130 MtrunA17_Chr7g0223031 63.993 611 215 4 1 609 1 608 0.0 790
Msa0548130 MtrunA17_Chr6g0462711 62.939 626 225 3 2 621 3 627 0.0 786
Msa0548130 MtrunA17_Chr7g0248131 63.578 626 218 8 1 619 1 623 0.0 785
Msa0548130 MtrunA17_Chr2g0298861 61.905 630 223 6 1 615 1 628 0.0 781
Msa0548130 MtrunA17_Chr7g0223631 63.607 621 207 6 1 619 1 604 0.0 780
Msa0548130 MtrunA17_Chr6g0462771 61.981 626 232 2 1 621 1 625 0.0 780
Msa0548130 MtrunA17_Chr7g0223781 63.089 615 221 3 1 613 1 611 0.0 779
Msa0548130 MtrunA17_Chr2g0287631 64.563 618 215 3 1 615 1 617 0.0 777
Msa0548130 MtrunA17_Chr7g0248151 63.360 625 221 6 1 620 1 622 0.0 776
Msa0548130 MtrunA17_Chr4g0040931 62.684 611 223 4 1 608 1 609 0.0 776
Msa0548130 MtrunA17_Chr7g0223701 62.843 619 216 6 1 608 1 616 0.0 775
Msa0548130 MtrunA17_Chr6g0462641 61.342 626 236 2 1 621 1 625 0.0 773
Msa0548130 MtrunA17_Chr7g0225671 63.192 614 218 7 6 615 13 622 0.0 773
Msa0548130 MtrunA17_Chr7g0223721 61.812 618 231 4 1 615 1 616 0.0 772
Msa0548130 MtrunA17_Chr7g0223851 62.682 619 224 6 1 615 10 625 0.0 764
Msa0548130 MtrunA17_Chr2g0296391 68.561 528 163 2 1 526 1 527 0.0 747
Msa0548130 MtrunA17_Chr7g0233301 60.548 621 208 6 1 617 1 588 0.0 743
Msa0548130 MtrunA17_Chr7g0223821 61.997 621 224 7 1 613 1 617 0.0 735
Msa0548130 MtrunA17_Chr7g0217971 61.296 602 204 5 1 600 1 575 0.0 734
Msa0548130 MtrunA17_Chr8g0390821 56.632 671 219 10 1 616 1 654 0.0 721
Msa0548130 MtrunA17_Chr6g0462611 64.014 553 196 2 63 615 13 562 0.0 721
Msa0548130 MtrunA17_Chr4g0012541 58.805 619 239 7 1 615 1 607 0.0 712
Msa0548130 MtrunA17_Chr7g0223831 59.411 611 211 8 1 606 1 579 0.0 701
Msa0548130 MtrunA17_Chr7g0223541 61.141 561 216 2 61 621 20 578 0.0 693
Msa0548130 MtrunA17_Chr7g0215261 57.997 619 218 7 1 614 1 582 0.0 692
Msa0548130 MtrunA17_Chr7g0228281 56.311 618 220 7 1 615 62 632 0.0 672
Msa0548130 MtrunA17_Chr2g0299921 84.576 389 58 1 4 392 1024 1410 0.0 671
Msa0548130 MtrunA17_Chr2g0299921 83.636 385 60 2 4 387 1408 1790 0.0 637
Msa0548130 MtrunA17_Chr2g0299851 83.721 387 61 1 1 387 1030 1414 0.0 667
Msa0548130 MtrunA17_Chr7g0223681 61.770 531 200 3 89 619 2 529 0.0 665
Msa0548130 MtrunA17_Chr2g0293601 63.969 519 150 3 1 517 1 484 0.0 654
Msa0548130 MtrunA17_Chr2g0299791 81.443 388 70 1 1 388 1034 1419 0.0 648
Msa0548130 MtrunA17_Chr2g0299821 72.000 450 112 3 1 436 1 450 0.0 638
Msa0548130 MtrunA17_Chr2g0299821 78.947 95 13 1 452 539 390 484 2.28e-39 151
Msa0548130 MtrunA17_Chr2g0331381 58.965 541 156 6 9 547 6 482 0.0 635
Msa0548130 MtrunA17_Chr2g0299881 77.057 401 86 3 6 402 1035 1433 0.0 627
Msa0548130 MtrunA17_Chr2g0299881 75.758 66 12 1 183 248 1414 1475 5.10e-22 101
Msa0548130 MtrunA17_Chr2g0293561 73.262 374 98 1 1 372 1 374 0.0 581
Msa0548130 MtrunA17_Chr1g0166551 50.977 614 183 8 6 615 2 501 0.0 579
Msa0548130 MtrunA17_Chr2g0279901 51.167 600 263 10 10 602 57 633 0.0 573
Msa0548130 MtrunA17_Chr6g0462861 50.159 628 277 11 1 615 1 605 0.0 568
Msa0548130 MtrunA17_Chr4g0049501 50.667 600 267 10 10 602 54 631 0.0 561
Msa0548130 MtrunA17_Chr2g0277021 47.819 642 306 13 10 641 72 694 0.0 533
Msa0548130 MtrunA17_Chr2g0293271 54.691 437 125 4 21 455 43 408 6.01e-161 468
Msa0548130 MtrunA17_Chr1g0151991 70.790 291 83 1 1 289 1 291 4.31e-149 433
Msa0548130 MtrunA17_Chr7g0245521 71.795 273 75 1 1 271 1 273 2.12e-141 412
Msa0548130 MtrunA17_Chr6g0462631 59.337 332 91 3 86 415 8 297 2.09e-124 370
Msa0548130 MtrunA17_Chr6g0462751 54.728 349 99 3 65 411 12 303 4.34e-119 357
Msa0548130 MtrunA17_Chr7g0223761 48.677 378 150 2 239 615 3 337 1.24e-117 355
Msa0548130 MtrunA17_Chr7g0245531 57.962 314 130 2 303 616 2 313 5.47e-116 349
Msa0548130 MtrunA17_Chr1g0152001 54.882 297 129 5 313 608 1 293 3.66e-101 311
Msa0548130 MtrunA17_Chr1g0188601 74.346 191 49 0 90 280 1 191 4.82e-99 301
Msa0548130 MtrunA17_Chr5g0394391 36.581 503 292 10 9 494 29 521 5.07e-97 309
Msa0548130 MtrunA17_Chr7g0223671 70.811 185 54 0 72 256 8 192 1.43e-92 285
Msa0548130 MtrunA17_Chr1g0188611 53.872 297 127 4 320 615 1 288 1.51e-86 272
Msa0548130 MtrunA17_Chr6g0462791 71.023 176 50 1 106 281 1 175 1.03e-82 258
Msa0548130 MtrunA17_Chr2g0331901 33.406 461 289 5 10 457 4 459 1.53e-82 278
Msa0548130 MtrunA17_Chr4g0004331 34.475 438 271 4 10 434 4 438 1.18e-81 275
Msa0548130 MtrunA17_Chr5g0444001 34.426 427 271 4 10 430 4 427 2.27e-78 259
Msa0548130 MtrunA17_Chr3g0098761 33.810 420 273 4 10 428 4 419 2.03e-75 257
Msa0548130 MtrunA17_Chr7g0223771 72.549 153 42 0 92 244 2 154 1.91e-74 236
Msa0548130 MtrunA17_Chr5g0394221 42.638 326 175 5 265 587 35 351 3.38e-70 232
Msa0548130 MtrunA17_Chr4g0046881 72.340 141 36 1 133 273 14 151 3.07e-68 219
Msa0548130 MtrunA17_Chr4g0064621 33.984 512 306 11 9 496 140 643 3.01e-67 233
Msa0548130 MtrunA17_Chr4g0060241 32.143 504 307 14 10 496 21 506 4.09e-67 229
Msa0548130 MtrunA17_Chr6g0462691 69.014 142 44 0 320 461 1 142 2.04e-62 204
Msa0548130 MtrunA17_Chr6g0462701 62.651 166 38 1 103 268 27 168 1.71e-61 202
Msa0548130 MtrunA17_Chr5g0442171 37.463 339 181 9 168 491 1 323 5.88e-60 203
Msa0548130 MtrunA17_Chr3g0120101 26.748 572 374 12 10 544 27 590 9.25e-59 212
Msa0548130 MtrunA17_Chr7g0223861 70.992 131 38 0 76 206 4 134 3.63e-58 192
Msa0548130 MtrunA17_Chr7g0245541 48.259 201 103 1 416 616 1 200 1.47e-53 182
Msa0548130 MtrunA17_Chr5g0394231 39.207 227 87 4 72 297 5 181 1.32e-43 154
Msa0548130 MtrunA17_Chr6g0481391 59.836 122 46 1 36 157 13 131 1.21e-39 142
Msa0548130 MtrunA17_Chr4g0046871 71.429 98 27 1 337 434 9 105 1.65e-39 140
Msa0548130 MtrunA17_Chr6g0462621 76.056 71 17 0 1 71 1 71 3.77e-32 119
Msa0548130 MtrunA17_Chr7g0223691 78.082 73 14 1 1 71 1 73 9.24e-32 117
Msa0548130 MtrunA17_Chr1g0188591 75.342 73 16 1 1 71 1 73 2.79e-30 114
Msa0548130 MtrunA17_Chr2g0284661 71.084 83 24 0 366 448 61 143 3.16e-30 115
Msa0548130 MtrunA17_Chr2g0284661 50.820 61 28 1 223 281 5 65 1.65e-11 62.4
Msa0548130 MtrunA17_Chr6g0481381 70.270 74 22 0 174 247 1 74 4.80e-30 117
Msa0548130 MtrunA17_Chr6g0481381 37.374 99 61 1 515 612 75 173 6.59e-11 62.0
Msa0548130 MtrunA17_Chr7g0223881 61.333 75 28 1 286 360 9 82 5.64e-26 102
Msa0548130 MtrunA17_Chr7g0223791 64.634 82 21 3 54 135 18 91 2.90e-24 100
Msa0548130 MtrunA17_Chr7g0223791 46.739 92 49 0 482 573 90 181 5.10e-19 85.1
Msa0548130 MtrunA17_Chr2g0299941 76.667 60 14 0 398 457 23 82 1.45e-23 95.1
Msa0548130 MtrunA17_Chr7g0221651 53.571 84 39 0 213 296 1 84 3.16e-23 94.4
Msa0548130 MtrunA17_Chr4g0064651 46.739 92 49 0 396 487 29 120 1.91e-22 93.2
Msa0548130 MtrunA17_Chr2g0299891 93.023 43 3 0 313 355 1 43 1.48e-21 89.0
Msa0548130 MtrunA17_Chr7g0223871 38.889 126 76 1 491 615 7 132 1.01e-18 83.2
Msa0548130 MtrunA17_Chr6g0462801 64.615 65 18 1 320 379 1 65 1.78e-18 80.1
Msa0548130 MtrunA17_Chr5g0442161 31.126 151 99 3 9 155 28 177 2.60e-15 74.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0548130 AT3G12580.1 92.320 651 47 2 1 649 1 650 0.0 1221
Msa0548130 AT5G02500.1 90.658 653 55 2 1 649 1 651 0.0 1213
Msa0548130 AT3G09440.1 90.783 651 56 2 1 649 1 649 0.0 1202
Msa0548130 AT3G09440.4 90.783 651 56 2 1 649 1 649 0.0 1202
Msa0548130 AT3G09440.2 90.783 651 56 2 1 649 1 649 0.0 1202
Msa0548130 AT3G09440.3 90.783 651 56 2 1 649 1 649 0.0 1202
Msa0548130 AT5G02490.1 89.297 654 64 2 1 649 1 653 0.0 1196
Msa0548130 AT1G16030.1 84.746 649 96 2 1 649 1 646 0.0 1137
Msa0548130 AT1G56410.1 87.399 619 76 1 1 619 1 617 0.0 1122
Msa0548130 AT5G42020.1 64.895 621 212 4 7 625 35 651 0.0 827
Msa0548130 AT5G42020.3 64.895 621 212 4 7 625 40 656 0.0 826
Msa0548130 AT5G28540.1 65.472 614 206 4 7 618 35 644 0.0 825
Msa0548130 AT1G09080.1 62.806 613 223 3 7 618 50 658 0.0 782
Msa0548130 AT1G09080.2 62.806 613 223 3 7 618 40 648 0.0 782
Msa0548130 AT5G02500.2 92.893 394 28 0 1 394 1 394 0.0 771
Msa0548130 AT5G02500.2 76.087 138 27 2 516 649 386 521 5.86e-54 193
Msa0548130 AT5G42020.2 58.615 621 196 9 7 625 35 596 0.0 695
Msa0548130 AT5G09590.1 51.858 592 259 9 10 594 60 632 0.0 560
Msa0548130 AT4G24280.1 49.251 601 281 10 10 605 81 662 0.0 538
Msa0548130 AT5G49910.1 48.315 623 295 11 10 624 81 684 0.0 535
Msa0548130 AT4G37910.1 52.076 578 251 9 10 580 55 613 0.0 531
Msa0548130 AT4G37910.2 52.076 578 251 9 10 580 55 613 0.0 531
Msa0548130 AT2G32120.1 34.180 512 309 9 9 508 30 525 1.58e-87 284
Msa0548130 AT2G32120.2 34.180 512 309 9 9 508 30 525 1.58e-87 284
Msa0548130 AT1G79930.2 35.388 438 267 4 10 434 4 438 3.00e-86 286
Msa0548130 AT1G79930.1 35.388 438 267 4 10 434 4 438 4.02e-86 287
Msa0548130 AT1G79920.1 35.388 438 267 4 10 434 4 438 5.09e-85 282
Msa0548130 AT1G79920.3 35.388 438 267 4 10 434 4 438 7.32e-85 281
Msa0548130 AT1G79920.2 35.388 438 267 4 10 434 4 438 7.32e-85 281
Msa0548130 AT1G79920.4 35.388 438 267 4 10 434 4 438 7.32e-85 281
Msa0548130 AT1G11660.4 27.515 676 401 12 10 619 4 656 6.21e-84 280
Msa0548130 AT1G11660.3 27.515 676 401 12 10 619 4 656 6.21e-84 280
Msa0548130 AT1G11660.1 27.515 676 401 12 10 619 4 656 6.21e-84 280
Msa0548130 AT1G11660.2 27.613 641 375 12 45 619 2 619 1.10e-75 257
Msa0548130 AT4G16660.2 25.487 718 423 16 5 615 46 758 1.87e-62 223
Msa0548130 AT4G16660.1 25.766 718 421 17 5 615 22 734 2.87e-61 219

Find 176 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCATAATCTCACTAAATTT+TGG 0.083756 4_1:-41840881 None:intergenic
GGTAGGAATGGTTGTGTTTC+TGG 0.103620 4_1:-41842805 None:intergenic
AAGGATGCCGGTGTCATTTC+TGG 0.156592 4_1:+41842030 Msa0548130:CDS
CCCAAAATTTAGTGAGATTA+TGG 0.173453 4_1:+41840880 Msa0548130:exon
CATCTTCTCAATTTCCTCTT+TGG 0.183600 4_1:-41843093 None:intergenic
TCTTGAATTCTTGAACAAAA+TGG 0.242578 4_1:-41842278 None:intergenic
AGCCTGTGGAGAAGTGTTTA+AGG 0.261872 4_1:+41842505 Msa0548130:CDS
CCAACCTGGTGTGCTGATTC+AGG 0.309133 4_1:+41842863 Msa0548130:CDS
TCAGATGCTAAGCGATTAAT+TGG 0.310966 4_1:+41841754 Msa0548130:intron
TACTCCTCTCTCCCTTGGTT+TGG 0.315513 4_1:+41842749 Msa0548130:CDS
TTAATCAACTTCCTCAATCT+TGG 0.333860 4_1:-41843471 None:intergenic
TGAAAACACCTGCTCCTTCT+TGG 0.336551 4_1:-41842826 None:intergenic
ACCTGCGATTGGAATCGATT+TGG 0.340588 4_1:+41840919 Msa0548130:CDS
TGGCCGTTCAAGGTCATTCC+TGG 0.343735 4_1:+41841820 Msa0548130:CDS
GCTGGCAGTGGAAGCGGTGC+TGG 0.344996 4_1:+41843446 Msa0548130:CDS
CCTGCGATTGGAATCGATTT+GGG 0.346174 4_1:+41840920 Msa0548130:CDS
CCTGATGAGGCTGTTGCTTA+TGG 0.347444 4_1:+41842654 Msa0548130:CDS
TGCAAAAGTTGTTGAACCTT+GGG 0.350273 4_1:-41842591 None:intergenic
ATTAAGCTAAACTTTGTTCA+TGG 0.354002 4_1:+41843489 Msa0548130:exon
TACTGCTGGTGATACACATT+TGG 0.354173 4_1:+41842224 Msa0548130:CDS
TGATACATCCTAGCAATGAT+AGG 0.362378 4_1:-41843374 None:intergenic
AGCTCAATATGGATTTGTTT+AGG 0.363242 4_1:+41842472 Msa0548130:CDS
AACTTTGTTCATGGGATTGA+TGG 0.364747 4_1:+41843498 Msa0548130:three_prime_UTR
GTACATGATGTTGTTCTTGT+TGG 0.372027 4_1:+41842552 Msa0548130:CDS
GTAGGAATGGTTGTGTTTCT+GGG 0.381013 4_1:-41842804 None:intergenic
TTATAGTTCACCACAATCAT+AGG 0.383446 4_1:-41841856 None:intergenic
CCATAAGCAACAGCCTCATC+AGG 0.383744 4_1:-41842654 None:intergenic
CATATTCATGTGTTGGTGTT+TGG 0.389658 4_1:+41840948 Msa0548130:CDS
GGATTTGTTTAGGAAGTGTA+TGG 0.401845 4_1:+41842482 Msa0548130:CDS
TTACCGAAAACAGTGTTAGT+AGG 0.404952 4_1:-41841094 None:intergenic
CCTGCTGCTGGCAGTGGAAG+CGG 0.405341 4_1:+41843440 Msa0548130:CDS
GATGTTACTCCTCTCTCCCT+TGG 0.408263 4_1:+41842744 Msa0548130:CDS
CTCAATCTTCTAAGAGCCCT+TGG 0.408771 4_1:-41842330 None:intergenic
CAAGGTGCTGGTGGTGATGC+TGG 0.412390 4_1:+41843395 Msa0548130:CDS
CATGAAATTGTGGCCGTTCA+AGG 0.412524 4_1:+41841810 Msa0548130:CDS
CATGATGTTGTTCTTGTTGG+TGG 0.420045 4_1:+41842555 Msa0548130:CDS
GAGATTGCAGAGGCTTATCT+TGG 0.422742 4_1:+41841940 Msa0548130:CDS
TGAAATTGATTCATTGTTTG+AGG 0.423673 4_1:+41842407 Msa0548130:CDS
GGAAAGCCTACCCTTGTCAT+TGG 0.428458 4_1:-41843072 None:intergenic
GACTCGTGATAACAATTTGT+TGG 0.428667 4_1:+41842908 Msa0548130:CDS
CAAAGAGGAAATTGAGAAGA+TGG 0.433532 4_1:+41843094 Msa0548130:CDS
GGGTATCCCTCCTGCTCCAA+GGG 0.435715 4_1:+41842947 Msa0548130:CDS
TAGGAGAATCAGCGATGTTT+CGG 0.437308 4_1:+41841777 Msa0548130:CDS
TTGCAAAAGTTGTTGAACCT+TGG 0.441982 4_1:-41842592 None:intergenic
TAATCAACTTCCTCAATCTT+GGG 0.446332 4_1:-41843470 None:intergenic
TTGGGCAAATTTGAGTTGTC+GGG 0.450094 4_1:+41842927 Msa0548130:CDS
TGGAGAGGCTGATGAATTTG+AGG 0.450186 4_1:+41843319 Msa0548130:CDS
TCCCTTGGTTTGGAGACTGC+AGG 0.450627 4_1:+41842759 Msa0548130:CDS
GACAAGAAGGCTACAAGTAC+TGG 0.452597 4_1:+41842111 Msa0548130:CDS
CTGCTCCTTCTTGGTAGGAA+TGG 0.452909 4_1:-41842817 None:intergenic
GTTGGGCAAATTTGAGTTGT+CGG 0.456584 4_1:+41842926 Msa0548130:CDS
CGGGTATCCCTCCTGCTCCA+AGG 0.460140 4_1:+41842946 Msa0548130:CDS
ACTGCTGGTGATACACATTT+GGG 0.462462 4_1:+41842225 Msa0548130:CDS
GATGGTCCTGCTGCTGGCAG+TGG 0.462691 4_1:+41843434 Msa0548130:CDS
ACTGCCGCTGCTATTGCCTA+TGG 0.464087 4_1:+41842084 Msa0548130:CDS
AGTGAGATTATGGCTGGAAA+AGG 0.464749 4_1:+41840890 Msa0548130:exon
GATGAAGATGGTCCTGCTGC+TGG 0.465181 4_1:+41843428 Msa0548130:CDS
ACTCGTGATAACAATTTGTT+GGG 0.474189 4_1:+41842909 Msa0548130:CDS
TGAGATCATAGCAAACGATC+AGG 0.474343 4_1:+41840985 Msa0548130:CDS
GTGTTTAAGGGATGCTAAGA+TGG 0.480423 4_1:+41842518 Msa0548130:CDS
GAAAATTGAAGATGCTATTG+AGG 0.480599 4_1:+41843265 Msa0548130:CDS
CAATGAATCAATTTCAATGG+TGG 0.482628 4_1:-41842400 None:intergenic
TTAAGCTAAACTTTGTTCAT+GGG 0.484479 4_1:+41843490 Msa0548130:exon
AAATTTAGTGAGATTATGGC+TGG 0.487985 4_1:+41840884 Msa0548130:exon
ATAGGCAATAGCAGCGGCAG+TGG 0.488775 4_1:-41842082 None:intergenic
CCGCTTCCACTGCCAGCAGC+AGG 0.490175 4_1:-41843440 None:intergenic
TATTGAGGAAGGTATCTTTG+AGG 0.490881 4_1:+41842194 Msa0548130:CDS
ACTGATACGGAACGTTTGAT+CGG 0.491310 4_1:+41841040 Msa0548130:CDS
GCAAGATTTCTTCAATGGAA+AGG 0.494283 4_1:+41842611 Msa0548130:CDS
TGTTTCACTTCTTACTATTG+AGG 0.495641 4_1:+41842179 Msa0548130:CDS
TCAGTAAAAGCAACATAAGA+AGG 0.496937 4_1:-41841022 None:intergenic
AAACAATGAATCAATTTCAA+TGG 0.497295 4_1:-41842403 None:intergenic
ATTCAAGAGGAAGAATAAGA+AGG 0.502948 4_1:+41842293 Msa0548130:CDS
GAAATTGATTCATTGTTTGA+GGG 0.510562 4_1:+41842408 Msa0548130:CDS
TGCCTATGGTCTTGACAAGA+AGG 0.512155 4_1:+41842098 Msa0548130:CDS
TGCTGAAGAGATATCTTCCA+TGG 0.512232 4_1:+41841900 Msa0548130:CDS
TGAGGAACACCCCTTGGAGC+AGG 0.513433 4_1:-41842957 None:intergenic
CTGCTTGTGAGAGAGCTAAG+AGG 0.514038 4_1:+41842355 Msa0548130:CDS
CATGGTTCTGATGAAGATGA+AGG 0.516950 4_1:+41841918 Msa0548130:CDS
TACCGAAAACAGTGTTAGTA+GGG 0.517002 4_1:-41841093 None:intergenic
AAGAATAAGAAGGATATTAG+CGG 0.517633 4_1:+41842303 Msa0548130:CDS
ACGTTGTGAATCGTTGAAGT+AGG 0.520100 4_1:-41841998 None:intergenic
ATAGGTTTGTCAGCAGGGCC+AGG 0.524557 4_1:-41841838 None:intergenic
GGAAATTGAGAAGATGGTGC+AGG 0.525352 4_1:+41843100 Msa0548130:CDS
ATGCTAAGCGATTAATTGGT+AGG 0.528922 4_1:+41841758 Msa0548130:CDS
AAAATTGAAGATGCTATTGA+GGG 0.529908 4_1:+41843266 Msa0548130:CDS
CGGTGCTGGTCCCAAGATTG+AGG 0.530030 4_1:+41843460 Msa0548130:CDS
TGAGGACAAGATGAAGGAGC+TGG 0.532976 4_1:+41843337 Msa0548130:CDS
CACCGCTCAAAATAGCAGCT+TGG 0.534910 4_1:-41842686 None:intergenic
GGGCCAGGAATGACCTTGAA+CGG 0.539439 4_1:-41841823 None:intergenic
AAAGGTGAAGGACCTGCGAT+TGG 0.542152 4_1:+41840908 Msa0548130:CDS
CAATCATAGGTTTGTCAGCA+GGG 0.542843 4_1:-41841843 None:intergenic
ACTTTGTTCATGGGATTGAT+GGG 0.542929 4_1:+41843499 Msa0548130:three_prime_UTR
AGGATATTAGCGGAAATCCA+AGG 0.545644 4_1:+41842313 Msa0548130:CDS
CTTGGTTTGGAGACTGCAGG+AGG 0.546406 4_1:+41842762 Msa0548130:CDS
TGTGAATCGTTGAAGTAGGC+AGG 0.547550 4_1:-41841994 None:intergenic
ACAATCATAGGTTTGTCAGC+AGG 0.547735 4_1:-41841844 None:intergenic
CCCAAATCGATTCCAATCGC+AGG 0.548277 4_1:-41840920 None:intergenic
GCCTGTGGAGAAGTGTTTAA+GGG 0.550982 4_1:+41842506 Msa0548130:CDS
AATGGTGGTTTGAGCAGTAG+AGG 0.553384 4_1:-41842385 None:intergenic
TCAGCAGAGGACAAGACCAC+AGG 0.553510 4_1:+41843026 Msa0548130:CDS
GGGGCTATTCAATGGTTGGA+TGG 0.553727 4_1:+41843287 Msa0548130:CDS
TTTGCAACCCTATCATTGCT+AGG 0.554597 4_1:+41843366 Msa0548130:CDS
ATCATTGCTAGGATGTATCA+AGG 0.555326 4_1:+41843377 Msa0548130:CDS
TTCAAAACGAGCACGAGTAA+TGG 0.556929 4_1:-41842448 None:intergenic
TGGTGATGCTGGAGGAGCCA+TGG 0.557888 4_1:+41843406 Msa0548130:CDS
GTGATCTGAGGAACACCCCT+TGG 0.562871 4_1:-41842963 None:intergenic
TCCCTTAAACACTTCTCCAC+AGG 0.563569 4_1:-41842507 None:intergenic
GTCTCCAAACCAAGGGAGAG+AGG 0.567259 4_1:-41842753 None:intergenic
GGAACAACATATTCATGTGT+TGG 0.568612 4_1:+41840941 Msa0548130:CDS
GGAACACCCCTTGGAGCAGG+AGG 0.568941 4_1:-41842954 None:intergenic
GATACATCCTAGCAATGATA+GGG 0.569465 4_1:-41843373 None:intergenic
TAGGCAATAGCAGCGGCAGT+GGG 0.574624 4_1:-41842081 None:intergenic
AGCAGGACCATCTTCATCCA+TGG 0.574951 4_1:-41843423 None:intergenic
TGAATTTGAGGACAAGATGA+AGG 0.575031 4_1:+41843331 Msa0548130:CDS
GCTAGGATGTATCAAGGTGC+TGG 0.576167 4_1:+41843383 Msa0548130:CDS
AAAGTACAAGTCTGAGGACG+AGG 0.578393 4_1:+41843130 Msa0548130:CDS
CACAACCATTCCTACCAAGA+AGG 0.580287 4_1:+41842812 Msa0548130:CDS
CATTCCTACCAAGAAGGAGC+AGG 0.582400 4_1:+41842818 Msa0548130:CDS
CTTCATCTTCATCAGAACCA+TGG 0.583090 4_1:-41841917 None:intergenic
TGAGGGTAATGAGAAAGTAC+AGG 0.583385 4_1:+41842707 Msa0548130:CDS
TCACTTCTTACTATTGAGGA+AGG 0.584344 4_1:+41842183 Msa0548130:CDS
TTCTTGTTGGTGGATCGACT+AGG 0.585926 4_1:+41842565 Msa0548130:CDS
TTTCTGGACTCAATGTGTTG+AGG 0.587988 4_1:+41842046 Msa0548130:CDS
CAATCACCAATGACAAGGGT+AGG 0.589395 4_1:+41843066 Msa0548130:CDS
TGCTTTGACATTGATGCCAA+CGG 0.589749 4_1:+41842990 Msa0548130:CDS
TAGTAGGGTTCATAGCAACT+TGG 0.589835 4_1:-41841078 None:intergenic
TCCTGCAGTCTCCAAACCAA+GGG 0.590338 4_1:-41842760 None:intergenic
CTCCTGCAGTCTCCAAACCA+AGG 0.595424 4_1:-41842761 None:intergenic
ATGGGTGGTTCATGATTGCA+AGG 0.596040 4_1:+41843517 Msa0548130:three_prime_UTR
AAAACTATGCATACAACATG+AGG 0.597334 4_1:+41843189 Msa0548130:CDS
CAGCAGAGGACAAGACCACA+GGG 0.598328 4_1:+41843027 Msa0548130:CDS
TTGTTCATGGGATTGATGGG+TGG 0.598913 4_1:+41843502 Msa0548130:three_prime_UTR
AGCCTTCTTGTCAAGACCAT+AGG 0.599068 4_1:-41842100 None:intergenic
TGGTTGGATGGAAATCAACT+TGG 0.605882 4_1:+41843299 Msa0548130:CDS
TAGACCTGAATCAGCACACC+AGG 0.606163 4_1:-41842867 None:intergenic
CAAGGGTAGGCTTTCCAAAG+AGG 0.607535 4_1:+41843079 Msa0548130:CDS
TTTGAGGTGAAAGCTACTGC+TGG 0.609989 4_1:+41842210 Msa0548130:CDS
GGATATTAGCGGAAATCCAA+GGG 0.611329 4_1:+41842314 Msa0548130:CDS
TGAGACATTCAGAATACCGT+TGG 0.615112 4_1:-41843006 None:intergenic
ACCAGGTTGGTTATCAGAGT+AGG 0.621601 4_1:-41842850 None:intergenic
GACAAGATGAAGGAGCTGGA+GGG 0.622357 4_1:+41843341 Msa0548130:CDS
TACAGAGTGACATGAAATTG+TGG 0.624043 4_1:+41841800 Msa0548130:CDS
CGTCAAGCTACAAAGGATGC+CGG 0.624953 4_1:+41842018 Msa0548130:CDS
TTCACAACGTCAAGCTACAA+AGG 0.626174 4_1:+41842011 Msa0548130:CDS
TAAGAGCATCAACCCTGATG+AGG 0.628505 4_1:+41842641 Msa0548130:CDS
GAGATCATAGCAAACGATCA+GGG 0.629271 4_1:+41840986 Msa0548130:CDS
ATTATGGCTGGAAAAGGTGA+AGG 0.630083 4_1:+41840896 Msa0548130:exon
GGATGGAAATCAACTTGGAG+AGG 0.632319 4_1:+41843304 Msa0548130:CDS
TGCTGACAAACCTATGATTG+TGG 0.632591 4_1:+41841846 Msa0548130:CDS
GGAGGAGCCATGGATGAAGA+TGG 0.633207 4_1:+41843416 Msa0548130:CDS
CATAAGCAACAGCCTCATCA+GGG 0.633756 4_1:-41842653 None:intergenic
AACACCTGCTCCTTCTTGGT+AGG 0.634088 4_1:-41842822 None:intergenic
CCTGAATCAGCACACCAGGT+TGG 0.637157 4_1:-41842863 None:intergenic
GGACAAGATGAAGGAGCTGG+AGG 0.638733 4_1:+41843340 Msa0548130:CDS
ACCTACTCTGATAACCAACC+TGG 0.640169 4_1:+41842849 Msa0548130:CDS
TCAAAGCAAACAGTGATCTG+AGG 0.640283 4_1:-41842975 None:intergenic
GATTACAATCACCAATGACA+AGG 0.645567 4_1:+41843061 Msa0548130:CDS
GGTGCTGGTGGTGATGCTGG+AGG 0.646421 4_1:+41843398 Msa0548130:CDS
AGGATGTATCAAGGTGCTGG+TGG 0.649243 4_1:+41843386 Msa0548130:CDS
AATTGAAGATGCTATTGAGG+GGG 0.652473 4_1:+41843268 Msa0548130:CDS
TGCTGAAAAGTACAAGTCTG+AGG 0.657032 4_1:+41843124 Msa0548130:CDS
TGGATCGACTAGGATTCCCA+AGG 0.664968 4_1:+41842575 Msa0548130:CDS
TCTACGAAGGAGAAAGAACA+AGG 0.674145 4_1:+41842886 Msa0548130:CDS
GTGCTGATTCAGGTCTACGA+AGG 0.676079 4_1:+41842873 Msa0548130:CDS
TATTCTGAATGTCTCAGCAG+AGG 0.676206 4_1:+41843013 Msa0548130:CDS
GAAGATGAAGGAGATTGCAG+AGG 0.676674 4_1:+41841930 Msa0548130:CDS
TAGGAAGTGTATGGAGCCTG+TGG 0.676933 4_1:+41842491 Msa0548130:CDS
GGTATCCCTCCTGCTCCAAG+GGG 0.680067 4_1:+41842948 Msa0548130:CDS
ATTACAATCACCAATGACAA+GGG 0.682522 4_1:+41843062 Msa0548130:CDS
GAACACCCCTTGGAGCAGGA+GGG 0.684051 4_1:-41842953 None:intergenic
AAATTGAAGATGCTATTGAG+GGG 0.691800 4_1:+41843267 Msa0548130:CDS
TGTGGTGAACTATAAAGCTG+AGG 0.717089 4_1:+41841864 Msa0548130:CDS
ATTGAGTCCAGAAATGACAC+CGG 0.717355 4_1:-41842037 None:intergenic
AAGACCATAGGCAATAGCAG+CGG 0.731023 4_1:-41842088 None:intergenic
GCTGGTGATACACATTTGGG+AGG 0.733697 4_1:+41842228 Msa0548130:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTAAAAAAAACAGATTAA+TGG - chr4_1:41841327-41841346 None:intergenic 10.0%
!! TTGTACATATACATAATTAT+AGG + chr4_1:41841544-41841563 Msa0548130:intron 15.0%
!!! ATAAGGTAGAAAAGTATAAA+AGG + chr4_1:41841379-41841398 Msa0548130:intron 20.0%
!!! AAAAGTATAAAAGGTAGAAA+AGG + chr4_1:41841388-41841407 Msa0548130:intron 20.0%
!! AAAGTATAAAAGGTAGAAAA+GGG + chr4_1:41841389-41841408 Msa0548130:intron 20.0%
!! AAACAATGAATCAATTTCAA+TGG - chr4_1:41842406-41842425 None:intergenic 20.0%
!!! TGTTTTGATTTAGATAATGT+AGG + chr4_1:41843572-41843591 Msa0548130:three_prime_UTR 20.0%
!! CCATAATCTCACTAAATTTT+GGG - chr4_1:41840883-41840902 None:intergenic 25.0%
!!! TTATGTTGCTTTTACTGATA+CGG + chr4_1:41841027-41841046 Msa0548130:CDS 25.0%
!! TGAGAAGTAGTTTGTATATA+AGG + chr4_1:41841362-41841381 Msa0548130:intron 25.0%
!!! TGAAGATTTTGATAACAGAA+TGG + chr4_1:41842251-41842270 Msa0548130:CDS 25.0%
! TCTTGAATTCTTGAACAAAA+TGG - chr4_1:41842281-41842300 None:intergenic 25.0%
!! ATTTTGTTCAAGAATTCAAG+AGG + chr4_1:41842280-41842299 Msa0548130:CDS 25.0%
! AAGAATAAGAAGGATATTAG+CGG + chr4_1:41842303-41842322 Msa0548130:CDS 25.0%
!! TGAAATTGATTCATTGTTTG+AGG + chr4_1:41842407-41842426 Msa0548130:CDS 25.0%
!! GAAATTGATTCATTGTTTGA+GGG + chr4_1:41842408-41842427 Msa0548130:CDS 25.0%
! AAAATTGAAGATGCTATTGA+GGG + chr4_1:41843266-41843285 Msa0548130:CDS 25.0%
! ATTAAGCTAAACTTTGTTCA+TGG + chr4_1:41843489-41843508 Msa0548130:exon 25.0%
! TTAAGCTAAACTTTGTTCAT+GGG + chr4_1:41843490-41843509 Msa0548130:exon 25.0%
GAAAAACTGGGAAAAAACTA+GGG - chr4_1:41840855-41840874 None:intergenic 30.0%
AGAAAAACTGGGAAAAAACT+AGG - chr4_1:41840856-41840875 None:intergenic 30.0%
!! TTTTGGGAATGAGAAAAACT+GGG - chr4_1:41840867-41840886 None:intergenic 30.0%
!! ATTTTGGGAATGAGAAAAAC+TGG - chr4_1:41840868-41840887 None:intergenic 30.0%
CCCAAAATTTAGTGAGATTA+TGG + chr4_1:41840880-41840899 Msa0548130:exon 30.0%
! GCCATAATCTCACTAAATTT+TGG - chr4_1:41840884-41840903 None:intergenic 30.0%
AAATTTAGTGAGATTATGGC+TGG + chr4_1:41840884-41840903 Msa0548130:exon 30.0%
TCAGTAAAAGCAACATAAGA+AGG - chr4_1:41841025-41841044 None:intergenic 30.0%
! TGCTGATCATAATTTTGCAT+TGG + chr4_1:41841598-41841617 Msa0548130:intron 30.0%
TCAGAGCATAATAGTAGAAA+AGG + chr4_1:41841657-41841676 Msa0548130:intron 30.0%
CAGAGCATAATAGTAGAAAA+GGG + chr4_1:41841658-41841677 Msa0548130:intron 30.0%
GTTTCAGTATTTGTCAAGAA+TGG + chr4_1:41841690-41841709 Msa0548130:intron 30.0%
TTATAGTTCACCACAATCAT+AGG - chr4_1:41841859-41841878 None:intergenic 30.0%
!!! AACGTTCTTATTTTCGATCT+TGG + chr4_1:41842141-41842160 Msa0548130:CDS 30.0%
TGTTTCACTTCTTACTATTG+AGG + chr4_1:41842179-41842198 Msa0548130:CDS 30.0%
ATTCAAGAGGAAGAATAAGA+AGG + chr4_1:41842293-41842312 Msa0548130:CDS 30.0%
CAATGAATCAATTTCAATGG+TGG - chr4_1:41842403-41842422 None:intergenic 30.0%
! AGCTCAATATGGATTTGTTT+AGG + chr4_1:41842472-41842491 Msa0548130:CDS 30.0%
! CTTTTGCAAGATTTCTTCAA+TGG + chr4_1:41842606-41842625 Msa0548130:CDS 30.0%
! ACTCGTGATAACAATTTGTT+GGG + chr4_1:41842909-41842928 Msa0548130:CDS 30.0%
ATTACAATCACCAATGACAA+GGG + chr4_1:41843062-41843081 Msa0548130:CDS 30.0%
!!! ATAGTTTTCAAGTGCATTCT+TGG - chr4_1:41843177-41843196 None:intergenic 30.0%
AAAACTATGCATACAACATG+AGG + chr4_1:41843189-41843208 Msa0548130:CDS 30.0%
GAAAATTGAAGATGCTATTG+AGG + chr4_1:41843265-41843284 Msa0548130:CDS 30.0%
AAATTGAAGATGCTATTGAG+GGG + chr4_1:41843267-41843286 Msa0548130:CDS 30.0%
TAATCAACTTCCTCAATCTT+GGG - chr4_1:41843473-41843492 None:intergenic 30.0%
TTAATCAACTTCCTCAATCT+TGG - chr4_1:41843474-41843493 None:intergenic 30.0%
GGAACAACATATTCATGTGT+TGG + chr4_1:41840941-41840960 Msa0548130:CDS 35.0%
! CATATTCATGTGTTGGTGTT+TGG + chr4_1:41840948-41840967 Msa0548130:CDS 35.0%
! AACCCTACTAACACTGTTTT+CGG + chr4_1:41841091-41841110 Msa0548130:CDS 35.0%
TACCGAAAACAGTGTTAGTA+GGG - chr4_1:41841096-41841115 None:intergenic 35.0%
TTACCGAAAACAGTGTTAGT+AGG - chr4_1:41841097-41841116 None:intergenic 35.0%
CTGATTGTGTTTGCTTGAAT+TGG + chr4_1:41841726-41841745 Msa0548130:intron 35.0%
TCAGATGCTAAGCGATTAAT+TGG + chr4_1:41841754-41841773 Msa0548130:intron 35.0%
ATGCTAAGCGATTAATTGGT+AGG + chr4_1:41841758-41841777 Msa0548130:CDS 35.0%
TACAGAGTGACATGAAATTG+TGG + chr4_1:41841800-41841819 Msa0548130:CDS 35.0%
!!! GTTCTTATTTTCGATCTTGG+TGG + chr4_1:41842144-41842163 Msa0548130:CDS 35.0%
TCACTTCTTACTATTGAGGA+AGG + chr4_1:41842183-41842202 Msa0548130:CDS 35.0%
!! TATTGAGGAAGGTATCTTTG+AGG + chr4_1:41842194-41842213 Msa0548130:CDS 35.0%
!!! TCGTTTTGAAGAGCTCAATA+TGG + chr4_1:41842461-41842480 Msa0548130:CDS 35.0%
! GGATTTGTTTAGGAAGTGTA+TGG + chr4_1:41842482-41842501 Msa0548130:CDS 35.0%
GTACATGATGTTGTTCTTGT+TGG + chr4_1:41842552-41842571 Msa0548130:CDS 35.0%
TGCAAAAGTTGTTGAACCTT+GGG - chr4_1:41842594-41842613 None:intergenic 35.0%
TTGCAAAAGTTGTTGAACCT+TGG - chr4_1:41842595-41842614 None:intergenic 35.0%
GCAAGATTTCTTCAATGGAA+AGG + chr4_1:41842611-41842630 Msa0548130:CDS 35.0%
GACTCGTGATAACAATTTGT+TGG + chr4_1:41842908-41842927 Msa0548130:CDS 35.0%
GATTACAATCACCAATGACA+AGG + chr4_1:41843061-41843080 Msa0548130:CDS 35.0%
CATCTTCTCAATTTCCTCTT+TGG - chr4_1:41843096-41843115 None:intergenic 35.0%
CAAAGAGGAAATTGAGAAGA+TGG + chr4_1:41843094-41843113 Msa0548130:CDS 35.0%
AATTGAAGATGCTATTGAGG+GGG + chr4_1:41843268-41843287 Msa0548130:CDS 35.0%
TGAATTTGAGGACAAGATGA+AGG + chr4_1:41843331-41843350 Msa0548130:CDS 35.0%
GATACATCCTAGCAATGATA+GGG - chr4_1:41843376-41843395 None:intergenic 35.0%
TGATACATCCTAGCAATGAT+AGG - chr4_1:41843377-41843396 None:intergenic 35.0%
ATCATTGCTAGGATGTATCA+AGG + chr4_1:41843377-41843396 Msa0548130:CDS 35.0%
AACTTTGTTCATGGGATTGA+TGG + chr4_1:41843498-41843517 Msa0548130:three_prime_UTR 35.0%
ACTTTGTTCATGGGATTGAT+GGG + chr4_1:41843499-41843518 Msa0548130:three_prime_UTR 35.0%
AGTGAGATTATGGCTGGAAA+AGG + chr4_1:41840890-41840909 Msa0548130:exon 40.0%
ATTATGGCTGGAAAAGGTGA+AGG + chr4_1:41840896-41840915 Msa0548130:exon 40.0%
TGAGATCATAGCAAACGATC+AGG + chr4_1:41840985-41841004 Msa0548130:CDS 40.0%
GAGATCATAGCAAACGATCA+GGG + chr4_1:41840986-41841005 Msa0548130:CDS 40.0%
ACTGATACGGAACGTTTGAT+CGG + chr4_1:41841040-41841059 Msa0548130:CDS 40.0%
TAGTAGGGTTCATAGCAACT+TGG - chr4_1:41841081-41841100 None:intergenic 40.0%
CAAGCAAACACAATCAGCTA+AGG - chr4_1:41841723-41841742 None:intergenic 40.0%
TAGGAGAATCAGCGATGTTT+CGG + chr4_1:41841777-41841796 Msa0548130:CDS 40.0%
CAATCATAGGTTTGTCAGCA+GGG - chr4_1:41841846-41841865 None:intergenic 40.0%
ACAATCATAGGTTTGTCAGC+AGG - chr4_1:41841847-41841866 None:intergenic 40.0%
TGCTGACAAACCTATGATTG+TGG + chr4_1:41841846-41841865 Msa0548130:CDS 40.0%
TGTGGTGAACTATAAAGCTG+AGG + chr4_1:41841864-41841883 Msa0548130:CDS 40.0%
TGCTGAAGAGATATCTTCCA+TGG + chr4_1:41841900-41841919 Msa0548130:CDS 40.0%
CTTCATCTTCATCAGAACCA+TGG - chr4_1:41841920-41841939 None:intergenic 40.0%
!! CATGGTTCTGATGAAGATGA+AGG + chr4_1:41841918-41841937 Msa0548130:CDS 40.0%
ACGTTGTGAATCGTTGAAGT+AGG - chr4_1:41842001-41842020 None:intergenic 40.0%
TTCACAACGTCAAGCTACAA+AGG + chr4_1:41842011-41842030 Msa0548130:CDS 40.0%
ATTGAGTCCAGAAATGACAC+CGG - chr4_1:41842040-41842059 None:intergenic 40.0%
TTTCTGGACTCAATGTGTTG+AGG + chr4_1:41842046-41842065 Msa0548130:CDS 40.0%
!! CTTATTTTCGATCTTGGTGG+TGG + chr4_1:41842147-41842166 Msa0548130:CDS 40.0%
TACTGCTGGTGATACACATT+TGG + chr4_1:41842224-41842243 Msa0548130:CDS 40.0%
ACTGCTGGTGATACACATTT+GGG + chr4_1:41842225-41842244 Msa0548130:CDS 40.0%
AGGATATTAGCGGAAATCCA+AGG + chr4_1:41842313-41842332 Msa0548130:CDS 40.0%
GGATATTAGCGGAAATCCAA+GGG + chr4_1:41842314-41842333 Msa0548130:CDS 40.0%
TTCAAAACGAGCACGAGTAA+TGG - chr4_1:41842451-41842470 None:intergenic 40.0%
GTGTTTAAGGGATGCTAAGA+TGG + chr4_1:41842518-41842537 Msa0548130:CDS 40.0%
CATGATGTTGTTCTTGTTGG+TGG + chr4_1:41842555-41842574 Msa0548130:CDS 40.0%
! TGAGGGTAATGAGAAAGTAC+AGG + chr4_1:41842707-41842726 Msa0548130:CDS 40.0%
GTAGGAATGGTTGTGTTTCT+GGG - chr4_1:41842807-41842826 None:intergenic 40.0%
TCTACGAAGGAGAAAGAACA+AGG + chr4_1:41842886-41842905 Msa0548130:CDS 40.0%
!! GTTGGGCAAATTTGAGTTGT+CGG + chr4_1:41842926-41842945 Msa0548130:CDS 40.0%
!! TTGGGCAAATTTGAGTTGTC+GGG + chr4_1:41842927-41842946 Msa0548130:CDS 40.0%
TCAAAGCAAACAGTGATCTG+AGG - chr4_1:41842978-41842997 None:intergenic 40.0%
TGCTTTGACATTGATGCCAA+CGG + chr4_1:41842990-41843009 Msa0548130:CDS 40.0%
TGAGACATTCAGAATACCGT+TGG - chr4_1:41843009-41843028 None:intergenic 40.0%
TATTCTGAATGTCTCAGCAG+AGG + chr4_1:41843013-41843032 Msa0548130:CDS 40.0%
!!! AATCTTGTTTTTCTGCCCTG+TGG - chr4_1:41843045-41843064 None:intergenic 40.0%
! TGCTGAAAAGTACAAGTCTG+AGG + chr4_1:41843124-41843143 Msa0548130:CDS 40.0%
TGGTTGGATGGAAATCAACT+TGG + chr4_1:41843299-41843318 Msa0548130:CDS 40.0%
TTTGCAACCCTATCATTGCT+AGG + chr4_1:41843366-41843385 Msa0548130:CDS 40.0%
ACCTGCGATTGGAATCGATT+TGG + chr4_1:41840919-41840938 Msa0548130:CDS 45.0%
! CCTGCGATTGGAATCGATTT+GGG + chr4_1:41840920-41840939 Msa0548130:CDS 45.0%
CATGAAATTGTGGCCGTTCA+AGG + chr4_1:41841810-41841829 Msa0548130:CDS 45.0%
GAAGATGAAGGAGATTGCAG+AGG + chr4_1:41841930-41841949 Msa0548130:CDS 45.0%
GAGATTGCAGAGGCTTATCT+TGG + chr4_1:41841940-41841959 Msa0548130:CDS 45.0%
TGTGAATCGTTGAAGTAGGC+AGG - chr4_1:41841997-41842016 None:intergenic 45.0%
AAGACCATAGGCAATAGCAG+CGG - chr4_1:41842091-41842110 None:intergenic 45.0%
! TGCCTATGGTCTTGACAAGA+AGG + chr4_1:41842098-41842117 Msa0548130:CDS 45.0%
AGCCTTCTTGTCAAGACCAT+AGG - chr4_1:41842103-41842122 None:intergenic 45.0%
GACAAGAAGGCTACAAGTAC+TGG + chr4_1:41842111-41842130 Msa0548130:CDS 45.0%
! TTTGAGGTGAAAGCTACTGC+TGG + chr4_1:41842210-41842229 Msa0548130:CDS 45.0%
! CTCAATCTTCTAAGAGCCCT+TGG - chr4_1:41842333-41842352 None:intergenic 45.0%
AATGGTGGTTTGAGCAGTAG+AGG - chr4_1:41842388-41842407 None:intergenic 45.0%
!! AGCCTGTGGAGAAGTGTTTA+AGG + chr4_1:41842505-41842524 Msa0548130:CDS 45.0%
!! GCCTGTGGAGAAGTGTTTAA+GGG + chr4_1:41842506-41842525 Msa0548130:CDS 45.0%
TCCCTTAAACACTTCTCCAC+AGG - chr4_1:41842510-41842529 None:intergenic 45.0%
! TTCTTGTTGGTGGATCGACT+AGG + chr4_1:41842565-41842584 Msa0548130:CDS 45.0%
!! TAAGAGCATCAACCCTGATG+AGG + chr4_1:41842641-41842660 Msa0548130:CDS 45.0%
CATAAGCAACAGCCTCATCA+GGG - chr4_1:41842656-41842675 None:intergenic 45.0%
!!! GTCCAAGCTGCTATTTTGAG+CGG + chr4_1:41842684-41842703 Msa0548130:CDS 45.0%
GGTAGGAATGGTTGTGTTTC+TGG - chr4_1:41842808-41842827 None:intergenic 45.0%
CACAACCATTCCTACCAAGA+AGG + chr4_1:41842812-41842831 Msa0548130:CDS 45.0%
TGAAAACACCTGCTCCTTCT+TGG - chr4_1:41842829-41842848 None:intergenic 45.0%
ACCTACTCTGATAACCAACC+TGG + chr4_1:41842849-41842868 Msa0548130:CDS 45.0%
!! ACCAGGTTGGTTATCAGAGT+AGG - chr4_1:41842853-41842872 None:intergenic 45.0%
CAATCACCAATGACAAGGGT+AGG + chr4_1:41843066-41843085 Msa0548130:CDS 45.0%
GGAAATTGAGAAGATGGTGC+AGG + chr4_1:41843100-41843119 Msa0548130:CDS 45.0%
AAAGTACAAGTCTGAGGACG+AGG + chr4_1:41843130-41843149 Msa0548130:CDS 45.0%
CTATTGAGGGGGCTATTCAA+TGG + chr4_1:41843279-41843298 Msa0548130:CDS 45.0%
GGATGGAAATCAACTTGGAG+AGG + chr4_1:41843304-41843323 Msa0548130:CDS 45.0%
TGGAGAGGCTGATGAATTTG+AGG + chr4_1:41843319-41843338 Msa0548130:CDS 45.0%
TTGTTCATGGGATTGATGGG+TGG + chr4_1:41843502-41843521 Msa0548130:three_prime_UTR 45.0%
ATGGGTGGTTCATGATTGCA+AGG + chr4_1:41843517-41843536 Msa0548130:three_prime_UTR 45.0%
AAAGGTGAAGGACCTGCGAT+TGG + chr4_1:41840908-41840927 Msa0548130:CDS 50.0%
! CCCAAATCGATTCCAATCGC+AGG - chr4_1:41840923-41840942 None:intergenic 50.0%
CGTCAAGCTACAAAGGATGC+CGG + chr4_1:41842018-41842037 Msa0548130:CDS 50.0%
!! AAGGATGCCGGTGTCATTTC+TGG + chr4_1:41842030-41842049 Msa0548130:CDS 50.0%
GCTGGTGATACACATTTGGG+AGG + chr4_1:41842228-41842247 Msa0548130:CDS 50.0%
CTGCTTGTGAGAGAGCTAAG+AGG + chr4_1:41842355-41842374 Msa0548130:CDS 50.0%
! TAGGAAGTGTATGGAGCCTG+TGG + chr4_1:41842491-41842510 Msa0548130:CDS 50.0%
TGGATCGACTAGGATTCCCA+AGG + chr4_1:41842575-41842594 Msa0548130:CDS 50.0%
CCATAAGCAACAGCCTCATC+AGG - chr4_1:41842657-41842676 None:intergenic 50.0%
! CCTGATGAGGCTGTTGCTTA+TGG + chr4_1:41842654-41842673 Msa0548130:CDS 50.0%
CACCGCTCAAAATAGCAGCT+TGG - chr4_1:41842689-41842708 None:intergenic 50.0%
!!! AGCTGCTATTTTGAGCGGTG+AGG + chr4_1:41842689-41842708 Msa0548130:CDS 50.0%
!!! GCTGCTATTTTGAGCGGTGA+GGG + chr4_1:41842690-41842709 Msa0548130:CDS 50.0%
GATGTTACTCCTCTCTCCCT+TGG + chr4_1:41842744-41842763 Msa0548130:CDS 50.0%
TACTCCTCTCTCCCTTGGTT+TGG + chr4_1:41842749-41842768 Msa0548130:CDS 50.0%
TCCTGCAGTCTCCAAACCAA+GGG - chr4_1:41842763-41842782 None:intergenic 50.0%
CTGCTCCTTCTTGGTAGGAA+TGG - chr4_1:41842820-41842839 None:intergenic 50.0%
CATTCCTACCAAGAAGGAGC+AGG + chr4_1:41842818-41842837 Msa0548130:CDS 50.0%
AACACCTGCTCCTTCTTGGT+AGG - chr4_1:41842825-41842844 None:intergenic 50.0%
TAGACCTGAATCAGCACACC+AGG - chr4_1:41842870-41842889 None:intergenic 50.0%
!! GTGCTGATTCAGGTCTACGA+AGG + chr4_1:41842873-41842892 Msa0548130:CDS 50.0%
GGAAAGCCTACCCTTGTCAT+TGG - chr4_1:41843075-41843094 None:intergenic 50.0%
CAAGGGTAGGCTTTCCAAAG+AGG + chr4_1:41843079-41843098 Msa0548130:CDS 50.0%
! GGACGAGGAGCACAAAAAGA+AGG + chr4_1:41843145-41843164 Msa0548130:CDS 50.0%
! CGAGGAGCACAAAAAGAAGG+TGG + chr4_1:41843148-41843167 Msa0548130:CDS 50.0%
GGAGCACAAAAAGAAGGTGG+AGG + chr4_1:41843151-41843170 Msa0548130:CDS 50.0%
TGAGGGGGCTATTCAATGGT+TGG + chr4_1:41843283-41843302 Msa0548130:CDS 50.0%
GGGGCTATTCAATGGTTGGA+TGG + chr4_1:41843287-41843306 Msa0548130:CDS 50.0%
TGAGGACAAGATGAAGGAGC+TGG + chr4_1:41843337-41843356 Msa0548130:CDS 50.0%
GACAAGATGAAGGAGCTGGA+GGG + chr4_1:41843341-41843360 Msa0548130:CDS 50.0%
GCTAGGATGTATCAAGGTGC+TGG + chr4_1:41843383-41843402 Msa0548130:CDS 50.0%
!! AGGATGTATCAAGGTGCTGG+TGG + chr4_1:41843386-41843405 Msa0548130:CDS 50.0%
AGCAGGACCATCTTCATCCA+TGG - chr4_1:41843426-41843445 None:intergenic 50.0%
TGGCCGTTCAAGGTCATTCC+TGG + chr4_1:41841820-41841839 Msa0548130:CDS 55.0%
GGGCCAGGAATGACCTTGAA+CGG - chr4_1:41841826-41841845 None:intergenic 55.0%
ATAGGTTTGTCAGCAGGGCC+AGG - chr4_1:41841841-41841860 None:intergenic 55.0%
TAGGCAATAGCAGCGGCAGT+GGG - chr4_1:41842084-41842103 None:intergenic 55.0%
ATAGGCAATAGCAGCGGCAG+TGG - chr4_1:41842085-41842104 None:intergenic 55.0%
ACTGCCGCTGCTATTGCCTA+TGG + chr4_1:41842084-41842103 Msa0548130:CDS 55.0%
GTCTCCAAACCAAGGGAGAG+AGG - chr4_1:41842756-41842775 None:intergenic 55.0%
!! TCCCTTGGTTTGGAGACTGC+AGG + chr4_1:41842759-41842778 Msa0548130:CDS 55.0%
CTCCTGCAGTCTCCAAACCA+AGG - chr4_1:41842764-41842783 None:intergenic 55.0%
!! CTTGGTTTGGAGACTGCAGG+AGG + chr4_1:41842762-41842781 Msa0548130:CDS 55.0%
CCTGAATCAGCACACCAGGT+TGG - chr4_1:41842866-41842885 None:intergenic 55.0%
!! CCAACCTGGTGTGCTGATTC+AGG + chr4_1:41842863-41842882 Msa0548130:CDS 55.0%
GTGATCTGAGGAACACCCCT+TGG - chr4_1:41842966-41842985 None:intergenic 55.0%
TCAGCAGAGGACAAGACCAC+AGG + chr4_1:41843026-41843045 Msa0548130:CDS 55.0%
CAGCAGAGGACAAGACCACA+GGG + chr4_1:41843027-41843046 Msa0548130:CDS 55.0%
GGACAAGATGAAGGAGCTGG+AGG + chr4_1:41843340-41843359 Msa0548130:CDS 55.0%
GGAGGAGCCATGGATGAAGA+TGG + chr4_1:41843416-41843435 Msa0548130:CDS 55.0%
GATGAAGATGGTCCTGCTGC+TGG + chr4_1:41843428-41843447 Msa0548130:CDS 55.0%
GGGTATCCCTCCTGCTCCAA+GGG + chr4_1:41842947-41842966 Msa0548130:CDS 60.0%
GGTATCCCTCCTGCTCCAAG+GGG + chr4_1:41842948-41842967 Msa0548130:CDS 60.0%
GAACACCCCTTGGAGCAGGA+GGG - chr4_1:41842956-41842975 None:intergenic 60.0%
TGAGGAACACCCCTTGGAGC+AGG - chr4_1:41842960-41842979 None:intergenic 60.0%
!! CAAGGTGCTGGTGGTGATGC+TGG + chr4_1:41843395-41843414 Msa0548130:CDS 60.0%
! TGGTGATGCTGGAGGAGCCA+TGG + chr4_1:41843406-41843425 Msa0548130:CDS 60.0%
!! CGGTGCTGGTCCCAAGATTG+AGG + chr4_1:41843460-41843479 Msa0548130:CDS 60.0%
CGGGTATCCCTCCTGCTCCA+AGG + chr4_1:41842946-41842965 Msa0548130:CDS 65.0%
GGAACACCCCTTGGAGCAGG+AGG - chr4_1:41842957-41842976 None:intergenic 65.0%
!! GGTGCTGGTGGTGATGCTGG+AGG + chr4_1:41843398-41843417 Msa0548130:CDS 65.0%
GATGGTCCTGCTGCTGGCAG+TGG + chr4_1:41843434-41843453 Msa0548130:CDS 65.0%
CCTGCTGCTGGCAGTGGAAG+CGG + chr4_1:41843440-41843459 Msa0548130:CDS 65.0%
CCGCTTCCACTGCCAGCAGC+AGG - chr4_1:41843443-41843462 None:intergenic 70.0%
GCTGGCAGTGGAAGCGGTGC+TGG + chr4_1:41843446-41843465 Msa0548130:CDS 70.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 41840790 41843629 41840790 ID=Msa0548130;Name=Msa0548130
chr4_1 mRNA 41840790 41843629 41840790 ID=Msa0548130-mRNA-1;Parent=Msa0548130;Name=Msa0548130-mRNA-1;_AED=0.01;_eAED=0.01;_QI=109|1|1|1|1|1|2|136|649
chr4_1 exon 41840790 41841112 41840790 ID=Msa0548130-mRNA-1:exon:10737;Parent=Msa0548130-mRNA-1
chr4_1 exon 41841758 41843629 41841758 ID=Msa0548130-mRNA-1:exon:10738;Parent=Msa0548130-mRNA-1
chr4_1 five_prime_UTR 41840790 41840898 41840790 ID=Msa0548130-mRNA-1:five_prime_utr;Parent=Msa0548130-mRNA-1
chr4_1 CDS 41840899 41841112 41840899 ID=Msa0548130-mRNA-1:cds;Parent=Msa0548130-mRNA-1
chr4_1 CDS 41841758 41843493 41841758 ID=Msa0548130-mRNA-1:cds;Parent=Msa0548130-mRNA-1
chr4_1 three_prime_UTR 41843494 41843629 41843494 ID=Msa0548130-mRNA-1:three_prime_utr;Parent=Msa0548130-mRNA-1
Gene Sequence

>Msa0548130

ATGGCTGGAAAAGGTGAAGGACCTGCGATTGGAATCGATTTGGGAACAACATATTCATGTGTTGGTGTTTGGCAACATGATCGTGTTGAGATCATAGCAAACGATCAGGGTAACAGAACTACTCCTTCTTATGTTGCTTTTACTGATACGGAACGTTTGATCGGTGATGCTGCTAAGAACCAAGTTGCTATGAACCCTACTAACACTGTTTTCGATGCTAAGCGATTAATTGGTAGGAGAATCAGCGATGTTTCGGTACAGAGTGACATGAAATTGTGGCCGTTCAAGGTCATTCCTGGCCCTGCTGACAAACCTATGATTGTGGTGAACTATAAAGCTGAGGAGAAACAATTTTCTGCTGAAGAGATATCTTCCATGGTTCTGATGAAGATGAAGGAGATTGCAGAGGCTTATCTTGGTACAACGATTAAGAATGCTGTCGTAACTGTGCCTGCCTACTTCAACGATTCACAACGTCAAGCTACAAAGGATGCCGGTGTCATTTCTGGACTCAATGTGTTGAGGATCATTAATGAGCCCACTGCCGCTGCTATTGCCTATGGTCTTGACAAGAAGGCTACAAGTACTGGTGAGAAGAACGTTCTTATTTTCGATCTTGGTGGTGGAACTTTTGATGTTTCACTTCTTACTATTGAGGAAGGTATCTTTGAGGTGAAAGCTACTGCTGGTGATACACATTTGGGAGGTGAAGATTTTGATAACAGAATGGTGAACCATTTTGTTCAAGAATTCAAGAGGAAGAATAAGAAGGATATTAGCGGAAATCCAAGGGCTCTTAGAAGATTGAGAACTGCTTGTGAGAGAGCTAAGAGGACTCTTTCCTCTACTGCTCAAACCACCATTGAAATTGATTCATTGTTTGAGGGTATTGACTTTTACACAACCATTACTCGTGCTCGTTTTGAAGAGCTCAATATGGATTTGTTTAGGAAGTGTATGGAGCCTGTGGAGAAGTGTTTAAGGGATGCTAAGATGGATAAGAACAGTGTACATGATGTTGTTCTTGTTGGTGGATCGACTAGGATTCCCAAGGTTCAACAACTTTTGCAAGATTTCTTCAATGGAAAGGAGCTTTGTAAGAGCATCAACCCTGATGAGGCTGTTGCTTATGGCGCTGCAGTCCAAGCTGCTATTTTGAGCGGTGAGGGTAATGAGAAAGTACAGGATCTGTTGTTGCTTGATGTTACTCCTCTCTCCCTTGGTTTGGAGACTGCAGGAGGAGTCATGACTGTGTTAATTCCCAGAAACACAACCATTCCTACCAAGAAGGAGCAGGTGTTTTCAACCTACTCTGATAACCAACCTGGTGTGCTGATTCAGGTCTACGAAGGAGAAAGAACAAGGACTCGTGATAACAATTTGTTGGGCAAATTTGAGTTGTCGGGTATCCCTCCTGCTCCAAGGGGTGTTCCTCAGATCACTGTTTGCTTTGACATTGATGCCAACGGTATTCTGAATGTCTCAGCAGAGGACAAGACCACAGGGCAGAAAAACAAGATTACAATCACCAATGACAAGGGTAGGCTTTCCAAAGAGGAAATTGAGAAGATGGTGCAGGATGCTGAAAAGTACAAGTCTGAGGACGAGGAGCACAAAAAGAAGGTGGAGGCCAAGAATGCACTTGAAAACTATGCATACAACATGAGGAACACAATCAAAGATGACAAGATTGCTTCGAAGTTGTCTGCTGATGATAAGAAGAAAATTGAAGATGCTATTGAGGGGGCTATTCAATGGTTGGATGGAAATCAACTTGGAGAGGCTGATGAATTTGAGGACAAGATGAAGGAGCTGGAGGGCATTTGCAACCCTATCATTGCTAGGATGTATCAAGGTGCTGGTGGTGATGCTGGAGGAGCCATGGATGAAGATGGTCCTGCTGCTGGCAGTGGAAGCGGTGCTGGTCCCAAGATTGAGGAAGTTGATTAA
Protein sequence

>Msa0548130

MAGKGEGPAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRISDVSVQSDMKLWPFKVIPGPADKPMIVVNYKAEEKQFSAEEISSMVLMKMKEIAEAYLGTTIKNAVVTVPAYFNDSQRQATKDAGVISGLNVLRIINEPTAAAIAYGLDKKATSTGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTHLGGEDFDNRMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLFEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKNSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAGGVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFELSGIPPAPRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKMVQDAEKYKSEDEEHKKKVEAKNALENYAYNMRNTIKDDKIASKLSADDKKKIEDAIEGAIQWLDGNQLGEADEFEDKMKELEGICNPIIARMYQGAGGDAGGAMDEDGPAAGSGSGAGPKIEEVD*