AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0548300

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0548300 MtrunA17_Chr4g0031321 99.692 649 2 0 1 649 1 649 0.0 1330
Msa0548300 MtrunA17_Chr4g0031301 99.230 649 5 0 1 649 1 649 0.0 1322
Msa0548300 MtrunA17_Chr7g0262821 92.450 649 49 0 1 649 1 649 0.0 1236
Msa0548300 MtrunA17_Chr7g0223291 92.780 651 43 3 1 649 1 649 0.0 1236
Msa0548300 MtrunA17_Chr7g0223181 92.473 651 44 2 1 649 1 648 0.0 1227
Msa0548300 MtrunA17_Chr4g0073741 87.309 654 77 3 1 649 1 653 0.0 1154
Msa0548300 MtrunA17_Chr6g0462681 67.154 615 201 1 1 615 1 614 0.0 850
Msa0548300 MtrunA17_Chr7g0223751 66.667 621 202 4 1 619 1 618 0.0 842
Msa0548300 MtrunA17_Chr2g0284681 67.881 604 174 3 9 606 8 597 0.0 835
Msa0548300 MtrunA17_Chr6g0462601 66.071 616 208 1 1 616 1 615 0.0 834
Msa0548300 MtrunA17_Chr7g0223711 65.378 621 211 3 1 619 1 619 0.0 834
Msa0548300 MtrunA17_Chr2g0298851 65.913 619 204 6 1 615 1 616 0.0 818
Msa0548300 MtrunA17_Chr7g0221661 65.528 615 208 3 1 613 1 613 0.0 818
Msa0548300 MtrunA17_Chr7g0223741 66.667 597 195 3 1 595 1 595 0.0 818
Msa0548300 MtrunA17_Chr8g0350731 64.800 625 215 4 1 623 29 650 0.0 811
Msa0548300 MtrunA17_Chr5g0427901 64.013 628 211 4 1 615 1 626 0.0 810
Msa0548300 MtrunA17_Chr2g0293581 65.416 613 208 3 6 615 7 618 0.0 810
Msa0548300 MtrunA17_Chr8g0388761 65.961 614 203 4 7 618 36 645 0.0 808
Msa0548300 MtrunA17_Chr8g0388671 65.961 614 203 4 7 618 36 645 0.0 806
Msa0548300 MtrunA17_Chr7g0223731 65.296 608 206 4 1 606 1 605 0.0 804
Msa0548300 MtrunA17_Chr7g0233311 63.592 618 221 3 1 615 1 617 0.0 801
Msa0548300 MtrunA17_Chr7g0223801 63.371 617 220 5 1 615 1 613 0.0 798
Msa0548300 MtrunA17_Chr7g0223621 64.590 610 211 4 1 608 1 607 0.0 798
Msa0548300 MtrunA17_Chr7g0223031 64.484 611 212 4 1 609 1 608 0.0 796
Msa0548300 MtrunA17_Chr7g0223631 64.090 621 204 6 1 619 1 604 0.0 786
Msa0548300 MtrunA17_Chr7g0248131 63.738 626 217 8 1 619 1 623 0.0 785
Msa0548300 MtrunA17_Chr6g0462711 62.780 626 226 3 2 621 3 627 0.0 784
Msa0548300 MtrunA17_Chr7g0223781 63.415 615 219 3 1 613 1 611 0.0 783
Msa0548300 MtrunA17_Chr2g0287631 65.049 618 212 3 1 615 1 617 0.0 782
Msa0548300 MtrunA17_Chr4g0040931 63.175 611 220 4 1 608 1 609 0.0 781
Msa0548300 MtrunA17_Chr7g0248151 63.840 625 218 6 1 620 1 622 0.0 781
Msa0548300 MtrunA17_Chr7g0223701 63.328 619 213 6 1 608 1 616 0.0 780
Msa0548300 MtrunA17_Chr6g0462771 62.141 626 231 2 1 621 1 625 0.0 780
Msa0548300 MtrunA17_Chr2g0298861 61.905 630 223 6 1 615 1 628 0.0 780
Msa0548300 MtrunA17_Chr7g0223721 62.298 618 228 4 1 615 1 616 0.0 778
Msa0548300 MtrunA17_Chr7g0225671 63.518 614 216 7 6 615 13 622 0.0 777
Msa0548300 MtrunA17_Chr6g0462641 61.182 626 237 2 1 621 1 625 0.0 771
Msa0548300 MtrunA17_Chr7g0223851 63.005 619 222 6 1 615 10 625 0.0 768
Msa0548300 MtrunA17_Chr2g0296391 68.809 529 162 2 1 527 1 528 0.0 752
Msa0548300 MtrunA17_Chr7g0233301 60.709 621 207 6 1 617 1 588 0.0 747
Msa0548300 MtrunA17_Chr7g0223821 62.319 621 222 7 1 613 1 617 0.0 738
Msa0548300 MtrunA17_Chr7g0217971 61.462 602 203 5 1 600 1 575 0.0 734
Msa0548300 MtrunA17_Chr8g0390821 56.781 671 218 10 1 616 1 654 0.0 721
Msa0548300 MtrunA17_Chr6g0462611 64.195 553 195 2 63 615 13 562 0.0 720
Msa0548300 MtrunA17_Chr4g0012541 59.128 619 237 7 1 615 1 607 0.0 716
Msa0548300 MtrunA17_Chr7g0223831 59.738 611 209 8 1 606 1 579 0.0 704
Msa0548300 MtrunA17_Chr7g0223541 61.676 561 213 2 61 621 20 578 0.0 699
Msa0548300 MtrunA17_Chr7g0215261 57.997 619 218 7 1 614 1 582 0.0 693
Msa0548300 MtrunA17_Chr7g0228281 56.472 618 219 7 1 615 62 632 0.0 674
Msa0548300 MtrunA17_Chr7g0223681 62.335 531 197 3 89 619 2 529 0.0 671
Msa0548300 MtrunA17_Chr2g0299921 84.576 389 58 1 4 392 1024 1410 0.0 669
Msa0548300 MtrunA17_Chr2g0299921 83.636 385 60 2 4 387 1408 1790 0.0 635
Msa0548300 MtrunA17_Chr2g0299851 83.204 387 63 1 1 387 1030 1414 0.0 663
Msa0548300 MtrunA17_Chr2g0293601 64.355 519 148 3 1 517 1 484 0.0 657
Msa0548300 MtrunA17_Chr2g0299791 81.443 388 70 1 1 388 1034 1419 0.0 647
Msa0548300 MtrunA17_Chr2g0299821 71.778 450 113 3 1 436 1 450 0.0 634
Msa0548300 MtrunA17_Chr2g0299821 80.000 95 12 1 452 539 390 484 7.70e-40 152
Msa0548300 MtrunA17_Chr2g0331381 58.780 541 157 6 9 547 6 482 0.0 632
Msa0548300 MtrunA17_Chr2g0299881 77.057 401 86 3 6 402 1035 1433 0.0 625
Msa0548300 MtrunA17_Chr2g0299881 75.758 66 12 1 183 248 1414 1475 4.80e-22 101
Msa0548300 MtrunA17_Chr2g0293561 73.797 374 96 1 1 372 1 374 0.0 585
Msa0548300 MtrunA17_Chr1g0166551 51.140 614 182 8 6 615 2 501 0.0 582
Msa0548300 MtrunA17_Chr2g0279901 51.333 600 262 10 10 602 57 633 0.0 576
Msa0548300 MtrunA17_Chr6g0462861 50.318 628 276 11 1 615 1 605 0.0 567
Msa0548300 MtrunA17_Chr4g0049501 51.000 600 265 10 10 602 54 631 0.0 566
Msa0548300 MtrunA17_Chr2g0277021 47.975 642 305 13 10 641 72 694 0.0 536
Msa0548300 MtrunA17_Chr2g0293271 54.920 437 124 4 21 455 43 408 3.85e-162 471
Msa0548300 MtrunA17_Chr1g0151991 70.790 291 83 1 1 289 1 291 3.95e-149 433
Msa0548300 MtrunA17_Chr7g0245521 72.161 273 74 1 1 271 1 273 5.23e-142 414
Msa0548300 MtrunA17_Chr6g0462631 59.036 332 92 3 86 415 8 297 2.99e-123 367
Msa0548300 MtrunA17_Chr7g0223761 49.206 378 148 2 239 615 3 337 4.10e-119 358
Msa0548300 MtrunA17_Chr6g0462751 54.441 349 100 3 65 411 12 303 4.61e-118 354
Msa0548300 MtrunA17_Chr7g0245531 58.599 314 128 2 303 616 2 313 8.89e-118 353
Msa0548300 MtrunA17_Chr1g0152001 55.556 297 127 5 313 608 1 293 9.52e-103 315
Msa0548300 MtrunA17_Chr1g0188601 73.822 191 50 0 90 280 1 191 1.06e-98 300
Msa0548300 MtrunA17_Chr5g0394391 36.382 503 293 10 9 494 29 521 6.48e-97 308
Msa0548300 MtrunA17_Chr7g0223671 69.841 189 57 0 72 260 8 196 4.09e-93 286
Msa0548300 MtrunA17_Chr1g0188611 53.872 297 127 4 320 615 1 288 1.19e-85 270
Msa0548300 MtrunA17_Chr6g0462791 70.455 176 51 1 106 281 1 175 1.89e-82 257
Msa0548300 MtrunA17_Chr2g0331901 32.972 461 291 5 10 457 4 459 7.00e-81 273
Msa0548300 MtrunA17_Chr4g0004331 34.018 438 273 4 10 434 4 438 5.62e-80 271
Msa0548300 MtrunA17_Chr5g0444001 34.192 427 272 4 10 430 4 427 2.39e-77 256
Msa0548300 MtrunA17_Chr3g0098761 33.571 420 274 4 10 428 4 419 3.22e-75 256
Msa0548300 MtrunA17_Chr7g0223771 73.203 153 41 0 92 244 2 154 6.40e-75 237
Msa0548300 MtrunA17_Chr5g0394221 42.945 326 174 5 265 587 35 351 1.32e-71 235
Msa0548300 MtrunA17_Chr4g0046881 73.050 141 35 1 133 273 14 151 9.05e-69 221
Msa0548300 MtrunA17_Chr4g0060241 32.143 504 307 14 10 496 21 506 3.26e-67 229
Msa0548300 MtrunA17_Chr4g0064621 33.789 512 307 11 9 496 140 643 5.20e-67 232
Msa0548300 MtrunA17_Chr6g0462701 62.651 166 38 1 103 268 27 168 1.36e-61 202
Msa0548300 MtrunA17_Chr5g0442171 37.168 339 182 9 168 491 1 323 6.59e-60 203
Msa0548300 MtrunA17_Chr6g0462691 67.586 145 47 0 320 464 1 145 2.75e-58 193
Msa0548300 MtrunA17_Chr7g0223861 70.992 131 38 0 76 206 4 134 4.47e-58 192
Msa0548300 MtrunA17_Chr3g0120101 27.567 526 336 12 10 498 27 544 1.83e-57 208
Msa0548300 MtrunA17_Chr7g0245541 48.756 201 102 1 416 616 1 200 4.83e-54 183
Msa0548300 MtrunA17_Chr5g0394231 39.207 227 87 4 72 297 5 181 1.12e-43 154
Msa0548300 MtrunA17_Chr4g0046871 72.449 98 26 1 337 434 9 105 1.63e-40 143
Msa0548300 MtrunA17_Chr6g0481391 59.836 122 46 1 36 157 13 131 1.16e-39 142
Msa0548300 MtrunA17_Chr6g0462621 76.056 71 17 0 1 71 1 71 3.88e-32 119
Msa0548300 MtrunA17_Chr7g0223691 78.082 73 14 1 1 71 1 73 9.15e-32 117
Msa0548300 MtrunA17_Chr6g0481381 71.622 74 21 0 174 247 1 74 1.32e-30 118
Msa0548300 MtrunA17_Chr6g0481381 38.384 99 60 1 515 612 75 173 1.77e-11 63.5
Msa0548300 MtrunA17_Chr1g0188591 75.342 73 16 1 1 71 1 73 2.58e-30 114
Msa0548300 MtrunA17_Chr2g0284661 71.084 83 24 0 366 448 61 143 3.16e-30 115
Msa0548300 MtrunA17_Chr2g0284661 50.820 61 28 1 223 281 5 65 1.65e-11 62.4
Msa0548300 MtrunA17_Chr7g0223881 60.000 75 29 1 286 360 9 82 1.06e-24 98.2
Msa0548300 MtrunA17_Chr7g0223791 64.634 82 21 3 54 135 18 91 2.96e-24 100
Msa0548300 MtrunA17_Chr7g0223791 45.652 92 50 0 482 573 90 181 2.40e-18 83.2
Msa0548300 MtrunA17_Chr2g0299941 76.667 60 14 0 398 457 23 82 1.54e-23 95.1
Msa0548300 MtrunA17_Chr7g0221651 53.571 84 39 0 213 296 1 84 3.42e-23 94.0
Msa0548300 MtrunA17_Chr4g0064651 46.739 92 49 0 396 487 29 120 1.93e-22 93.2
Msa0548300 MtrunA17_Chr2g0299891 90.698 43 4 0 313 355 1 43 2.77e-20 85.1
Msa0548300 MtrunA17_Chr7g0223871 39.683 126 75 1 491 615 7 132 2.84e-19 84.7
Msa0548300 MtrunA17_Chr6g0462801 63.077 65 19 1 320 379 1 65 3.24e-17 76.6
Msa0548300 MtrunA17_Chr5g0442161 31.126 151 99 3 9 155 28 177 2.57e-15 74.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0548300 AT3G12580.1 92.473 651 46 2 1 649 1 650 0.0 1220
Msa0548300 AT5G02500.1 90.812 653 54 2 1 649 1 651 0.0 1211
Msa0548300 AT3G09440.1 91.244 651 53 2 1 649 1 649 0.0 1208
Msa0548300 AT3G09440.4 91.244 651 53 2 1 649 1 649 0.0 1208
Msa0548300 AT3G09440.2 91.244 651 53 2 1 649 1 649 0.0 1208
Msa0548300 AT3G09440.3 91.244 651 53 2 1 649 1 649 0.0 1208
Msa0548300 AT5G02490.1 89.144 654 65 2 1 649 1 653 0.0 1192
Msa0548300 AT1G16030.1 84.284 649 99 2 1 649 1 646 0.0 1130
Msa0548300 AT1G56410.1 87.237 619 77 1 1 619 1 617 0.0 1118
Msa0548300 AT5G42020.1 65.217 621 210 4 7 625 35 651 0.0 832
Msa0548300 AT5G42020.3 65.217 621 210 4 7 625 40 656 0.0 830
Msa0548300 AT5G28540.1 65.798 614 204 4 7 618 35 644 0.0 829
Msa0548300 AT1G09080.2 63.132 613 221 3 7 618 40 648 0.0 785
Msa0548300 AT1G09080.1 63.132 613 221 3 7 618 50 658 0.0 785
Msa0548300 AT5G02500.2 92.893 394 28 0 1 394 1 394 0.0 769
Msa0548300 AT5G02500.2 76.812 138 26 2 516 649 386 521 1.53e-54 195
Msa0548300 AT5G42020.2 62.545 558 184 8 7 550 35 581 0.0 674
Msa0548300 AT5G42020.2 51.724 87 41 1 540 625 510 596 1.40e-21 99.8
Msa0548300 AT5G09590.1 52.196 592 257 9 10 594 60 632 0.0 563
Msa0548300 AT4G24280.1 49.418 601 280 10 10 605 81 662 0.0 541
Msa0548300 AT5G49910.1 48.475 623 294 11 10 624 81 684 0.0 538
Msa0548300 AT4G37910.1 52.422 578 249 9 10 580 55 613 0.0 535
Msa0548300 AT4G37910.2 52.422 578 249 9 10 580 55 613 0.0 535
Msa0548300 AT2G32120.1 34.180 512 309 9 9 508 30 525 1.80e-88 286
Msa0548300 AT2G32120.2 34.180 512 309 9 9 508 30 525 1.80e-88 286
Msa0548300 AT1G79930.2 34.932 438 269 4 10 434 4 438 1.26e-84 282
Msa0548300 AT1G79930.1 34.932 438 269 4 10 434 4 438 1.64e-84 283
Msa0548300 AT1G79920.1 34.932 438 269 4 10 434 4 438 2.42e-83 278
Msa0548300 AT1G79920.3 34.932 438 269 4 10 434 4 438 3.44e-83 277
Msa0548300 AT1G79920.2 34.932 438 269 4 10 434 4 438 3.44e-83 277
Msa0548300 AT1G79920.4 34.932 438 269 4 10 434 4 438 3.44e-83 277
Msa0548300 AT1G11660.4 27.219 676 403 12 10 619 4 656 1.96e-82 276
Msa0548300 AT1G11660.3 27.219 676 403 12 10 619 4 656 1.96e-82 276
Msa0548300 AT1G11660.1 27.219 676 403 12 10 619 4 656 1.96e-82 276
Msa0548300 AT1G11660.2 27.301 641 377 12 45 619 2 619 2.55e-74 253
Msa0548300 AT4G16660.2 25.348 718 424 16 5 615 46 758 2.02e-62 223
Msa0548300 AT4G16660.1 25.627 718 422 17 5 615 22 734 3.89e-61 219

Find 165 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCTTCTCAATTTCCTCTT+TGG 0.183600 4_1:-42139535 None:intergenic
AAGGATGCCGGTGTCATTTC+TGG 0.230319 4_1:+42138472 Msa0548300:CDS
TCTTGAATTCTTGAACAAAA+TGG 0.242578 4_1:-42138720 None:intergenic
AGCCTGTTGAGAAGTGTTTA+AGG 0.251003 4_1:+42138947 Msa0548300:CDS
TGATACATCCTTGCAATGAT+AGG 0.282685 4_1:-42139816 None:intergenic
TACTCCTCTTTCCCTTGGTT+TGG 0.290944 4_1:+42139191 Msa0548300:CDS
GATTGTGTTCCTCATGTTGT+AGG 0.297657 4_1:-42139640 None:intergenic
TCAGATGCCAAGCGTTTAAT+TGG 0.329823 4_1:+42138196 Msa0548300:intron
TGGCCATTCAAGGTCATTCC+TGG 0.331072 4_1:+42138262 Msa0548300:CDS
ACTGCTGCTGCTATTGCTTA+TGG 0.331381 4_1:+42138526 Msa0548300:CDS
CGAAAACACCTGCTCCTTCT+TGG 0.338820 4_1:-42139268 None:intergenic
ACCTGCGATTGGAATCGATT+TGG 0.340588 4_1:+42137210 Msa0548300:CDS
GCTGGCAGTGGAAGTGGTGC+TGG 0.342439 4_1:+42139888 Msa0548300:CDS
CCTGCGATTGGAATCGATTT+GGG 0.346174 4_1:+42137211 Msa0548300:CDS
TACTGCTGGTGATACACATT+TGG 0.354173 4_1:+42138666 Msa0548300:CDS
CCTGATGAAGCCGTTGCATA+TGG 0.359843 4_1:+42139096 Msa0548300:CDS
TGCAAAAGTTGTTGAACCTT+CGG 0.362052 4_1:-42139033 None:intergenic
AATGATTGTGGTGAACTATA+AGG 0.363219 4_1:+42138300 Msa0548300:CDS
CCTGCTGCTGGCAGTGGAAG+TGG 0.369861 4_1:+42139882 Msa0548300:CDS
GTAGGAATGGTTGTGTTTCT+CGG 0.374272 4_1:-42139246 None:intergenic
GTTGATGATGTTGTTCTAGT+TGG 0.375499 4_1:+42138994 Msa0548300:CDS
CCAACCTGGTGTGTTGATTC+AGG 0.377108 4_1:+42139305 Msa0548300:CDS
CATATTCATGTGTTGGTGTT+TGG 0.389658 4_1:+42137239 Msa0548300:CDS
GGATTTGTTTAGAAAGTGTA+TGG 0.395555 4_1:+42138924 Msa0548300:CDS
TAGGCGAATCAGCGATGTTT+CGG 0.397395 4_1:+42138219 Msa0548300:CDS
TTATAGTTCACCACAATCAT+TGG 0.398480 4_1:-42138298 None:intergenic
TTACCGAAAACAGTGTTAGT+AGG 0.404952 4_1:-42137385 None:intergenic
GATGTTACTCCTCTTTCCCT+TGG 0.405687 4_1:+42139186 Msa0548300:CDS
GATGGTCCTGCTGCTGGCAG+TGG 0.411505 4_1:+42139876 Msa0548300:CDS
ACAATCATTGGTTTGTCAGC+AGG 0.411900 4_1:-42138286 None:intergenic
CAAGGTGCTGGTGGTGATGC+TGG 0.412390 4_1:+42139837 Msa0548300:CDS
CATGAAATTGTGGCCATTCA+AGG 0.415218 4_1:+42138252 Msa0548300:CDS
TCCCTTGGTTTGGAGACTGC+AGG 0.421609 4_1:+42139201 Msa0548300:CDS
GAGATTGCAGAGGCTTATCT+TGG 0.422742 4_1:+42138382 Msa0548300:CDS
GTTGGGCAAGTTTGAGTTGT+CGG 0.423204 4_1:+42139368 Msa0548300:CDS
TGAAATTGATTCATTGTTTG+AGG 0.423673 4_1:+42138849 Msa0548300:CDS
GGAAAGCCTACCCTTGTCAT+TGG 0.428458 4_1:-42139514 None:intergenic
TCCCTTAAACACTTCTCAAC+AGG 0.433425 4_1:-42138949 None:intergenic
CAAAGAGGAAATTGAGAAGA+TGG 0.433532 4_1:+42139536 Msa0548300:CDS
TTGGGCAAGTTTGAGTTGTC+GGG 0.437869 4_1:+42139369 Msa0548300:CDS
GATGAGGATGGTCCTGCTGC+TGG 0.439660 4_1:+42139870 Msa0548300:CDS
CATGTTGAGCTCTTCAAAAC+GGG 0.446462 4_1:-42138902 None:intergenic
CCATATGCAACGGCTTCATC+AGG 0.446831 4_1:-42139096 None:intergenic
CTGCTCCTTCTTGGTAGGAA+TGG 0.452909 4_1:-42139259 None:intergenic
CTGATGAAGCCGTTGCATAT+GGG 0.454888 4_1:+42139097 Msa0548300:CDS
GGGCATACCTCCTGCTCCAA+GGG 0.460951 4_1:+42139389 Msa0548300:CDS
ACTGCTGGTGATACACATTT+GGG 0.462462 4_1:+42138667 Msa0548300:CDS
TTATGTTGCTTTCACTGATA+CGG 0.463481 4_1:+42137318 Msa0548300:CDS
CATGGTTCTTATGAAGATGA+AGG 0.464118 4_1:+42138360 Msa0548300:CDS
AGAAATGACACCGGCATCCT+TGG 0.464569 4_1:-42138470 None:intergenic
AGTGAGATTATGGCTGGAAA+AGG 0.464749 4_1:+42137181 None:intergenic
CCATGTTGAGCTCTTCAAAA+CGG 0.468199 4_1:-42138903 None:intergenic
AGTACAGGATCTGTTGTTGC+TGG 0.469670 4_1:+42139164 Msa0548300:CDS
TGAGATCATAGCAAACGATC+AGG 0.474343 4_1:+42137276 Msa0548300:CDS
TCGCCTACCAATTAAACGCT+TGG 0.477726 4_1:-42138203 None:intergenic
CGGGCATACCTCCTGCTCCA+AGG 0.477998 4_1:+42139388 Msa0548300:CDS
GATGATGTTGTTCTAGTTGG+TGG 0.478696 4_1:+42138997 Msa0548300:CDS
GTGTTTAAGGGATGCTAAGA+TGG 0.480423 4_1:+42138960 Msa0548300:CDS
GAAAATTGAAGATGCTATTG+AGG 0.480599 4_1:+42139707 Msa0548300:CDS
ATTGGTTTGTCAGCAGGGCC+AGG 0.481800 4_1:-42138280 None:intergenic
CAATGAATCAATTTCAATGG+TGG 0.482628 4_1:-42138842 None:intergenic
CTCAATCTTCTCAGAGCCCT+CGG 0.484863 4_1:-42138772 None:intergenic
AAATTTAGTGAGATTATGGC+TGG 0.487985 4_1:+42137175 None:intergenic
TTCTAGTTGGTGGATCTACT+AGG 0.492016 4_1:+42139007 Msa0548300:CDS
GCCTGTTGAGAAGTGTTTAA+GGG 0.493521 4_1:+42138948 Msa0548300:CDS
GCAAGATTTCTTCAATGGAA+AGG 0.494283 4_1:+42139053 Msa0548300:CDS
GGGCCAGGAATGACCTTGAA+TGG 0.494864 4_1:-42138265 None:intergenic
TGTTTCACTTCTTACTATTG+AGG 0.495641 4_1:+42138621 Msa0548300:CDS
AAACAATGAATCAATTTCAA+TGG 0.497295 4_1:-42138845 None:intergenic
ATTCAAGAGGAAGAATAAGA+AGG 0.502948 4_1:+42138735 Msa0548300:CDS
GACTCGTGATAACAATCTGT+TGG 0.504268 4_1:+42139350 Msa0548300:CDS
ACTGATACGGAACGTTTGAT+CGG 0.504694 4_1:+42137331 Msa0548300:CDS
GATACATCCTTGCAATGATA+GGG 0.505117 4_1:-42139815 None:intergenic
GGAAATTGAGAAGATGGTGC+AGG 0.506093 4_1:+42139542 Msa0548300:CDS
CCACTTCCACTGCCAGCAGC+AGG 0.506743 4_1:-42139882 None:intergenic
GAAATTGATTCATTGTTTGA+GGG 0.510562 4_1:+42138850 Msa0548300:CDS
TGCTGAAGAGATATCTTCCA+TGG 0.512232 4_1:+42138342 Msa0548300:CDS
CAATCATTGGTTTGTCAGCA+GGG 0.512365 4_1:-42138285 None:intergenic
TGAGGAACACCCCTTGGAGC+AGG 0.513433 4_1:-42139399 None:intergenic
AGTAGAGGAAAGGGTCCTCT+TGG 0.513969 4_1:-42138812 None:intergenic
TCAGCAGAGGACAAGACTAC+AGG 0.515526 4_1:+42139468 Msa0548300:CDS
TACCGAAAACAGTGTTAGTA+GGG 0.517002 4_1:-42137384 None:intergenic
GTCTCCAAACCAAGGGAAAG+AGG 0.524557 4_1:-42139195 None:intergenic
ACTCGTGATAACAATCTGTT+GGG 0.525563 4_1:+42139351 Msa0548300:CDS
AAAATTGAAGATGCTATTGA+GGG 0.529908 4_1:+42139708 Msa0548300:CDS
AAAGTACAAGTCTGAGGATG+AGG 0.531693 4_1:+42139572 Msa0548300:CDS
TGAATTTGAAGACAAGATGA+AGG 0.531804 4_1:+42139773 Msa0548300:CDS
TGAAGACAAGATGAAGGAGC+TGG 0.532976 4_1:+42139779 Msa0548300:CDS
CTTGGTTTGGAGACTGCAGG+AGG 0.533555 4_1:+42139204 Msa0548300:CDS
AAAGGTGAAGGACCTGCGAT+TGG 0.542152 4_1:+42137199 Msa0548300:CDS
GATAAGAAAGCTACAAGTAC+CGG 0.542379 4_1:+42138553 Msa0548300:CDS
TCAGTGAAAGCAACATAAGA+AGG 0.544801 4_1:-42137313 None:intergenic
TGAGACATTTAAAATACCAT+TGG 0.546511 4_1:-42139448 None:intergenic
TGTGAATCATTGAAGTAAGC+AGG 0.547923 4_1:-42138436 None:intergenic
CCCAAATCGATTCCAATCGC+AGG 0.548277 4_1:-42137211 None:intergenic
AAGAATAAGAAGGATATCAG+TGG 0.551907 4_1:+42138745 Msa0548300:CDS
TGATGAAGCCGTTGCATATG+GGG 0.552611 4_1:+42139098 Msa0548300:CDS
ATGCCAAGCGTTTAATTGGT+AGG 0.552994 4_1:+42138200 Msa0548300:CDS
AATGGTGGTTTGAGCAGTAG+AGG 0.553384 4_1:-42138827 None:intergenic
GGGGCTATTCAATGGTTGGA+TGG 0.553727 4_1:+42139729 Msa0548300:CDS
GGTTTGAGCAGTAGAGGAAA+GGG 0.553832 4_1:-42138821 None:intergenic
CACCACTCAAAATAGCAGCT+TGG 0.555458 4_1:-42139128 None:intergenic
TGGTGATGCTGGAGGAGCCA+TGG 0.557888 4_1:+42139848 Msa0548300:CDS
ATCATTGCAAGGATGTATCA+AGG 0.560478 4_1:+42139819 Msa0548300:CDS
TTTGAGGTGAAAGCTACTGC+TGG 0.564230 4_1:+42138652 Msa0548300:CDS
TGCTTTGACATTGATGCCAA+TGG 0.565546 4_1:+42139432 Msa0548300:CDS
GTGATCTGAGGAACACCCCT+TGG 0.567797 4_1:-42139405 None:intergenic
GGAACAACATATTCATGTGT+TGG 0.568612 4_1:+42137232 Msa0548300:CDS
CAGCAGAGGACAAGACTACA+GGG 0.570449 4_1:+42139469 Msa0548300:CDS
CTTCATCTTCATAAGAACCA+TGG 0.578877 4_1:-42138359 None:intergenic
CACAACCATTCCTACCAAGA+AGG 0.580287 4_1:+42139254 Msa0548300:CDS
TGGTTTGAGCAGTAGAGGAA+AGG 0.580792 4_1:-42138822 None:intergenic
CATTCCTACCAAGAAGGAGC+AGG 0.582400 4_1:+42139260 Msa0548300:CDS
TCACTTCTTACTATTGAGGA+AGG 0.584344 4_1:+42138625 Msa0548300:CDS
AGCAGGACCATCCTCATCCA+TGG 0.585907 4_1:-42139865 None:intergenic
TGTATGAAGGCGAAAGAACA+AGG 0.586977 4_1:+42139328 Msa0548300:CDS
CAATCACCAATGACAAGGGT+AGG 0.589395 4_1:+42139508 Msa0548300:CDS
TAGTAGGGTTCATAGCAACT+TGG 0.589835 4_1:-42137369 None:intergenic
GCAAGGATGTATCAAGGTGC+TGG 0.591076 4_1:+42139825 Msa0548300:CDS
GACAAGATGAAGGAGCTGGA+GGG 0.594000 4_1:+42139783 Msa0548300:CDS
AAAACTATGCCTACAACATG+AGG 0.594809 4_1:+42139631 Msa0548300:CDS
TGAGGGAAATGAGAAAGTAC+AGG 0.596958 4_1:+42139149 Msa0548300:CDS
TCCTGCAGTCTCCAAACCAA+GGG 0.603057 4_1:-42139202 None:intergenic
TGCTGGAGGAGCCATGGATG+AGG 0.603777 4_1:+42139854 Msa0548300:CDS
CTCCTGCAGTCTCCAAACCA+AGG 0.604106 4_1:-42139203 None:intergenic
TGGTTGGATGGAAATCAACT+TGG 0.605882 4_1:+42139741 Msa0548300:CDS
CAAGGGTAGGCTTTCCAAAG+AGG 0.607535 4_1:+42139521 Msa0548300:CDS
TTTGCAACCCTATCATTGCA+AGG 0.610006 4_1:+42139808 Msa0548300:CDS
GTGTTGATTCAGGTGTATGA+AGG 0.610158 4_1:+42139315 Msa0548300:CDS
CGTCAAGCTACCAAGGATGC+CGG 0.612550 4_1:+42138460 Msa0548300:CDS
TGTGGTGAACTATAAGGCGG+AGG 0.616582 4_1:+42138306 Msa0548300:CDS
GACTGCTGCCCCATATGCAA+CGG 0.621746 4_1:-42139106 None:intergenic
TACAGAGCGACATGAAATTG+TGG 0.624043 4_1:+42138242 Msa0548300:CDS
TGGATCTACTAGGATTCCGA+AGG 0.625450 4_1:+42139017 Msa0548300:CDS
AGCTGAAAAGTACAAGTCTG+AGG 0.625787 4_1:+42139566 Msa0548300:CDS
ACCTACTCTGATAACCAACC+TGG 0.627630 4_1:+42139291 Msa0548300:CDS
GAGATCATAGCAAACGATCA+GGG 0.629271 4_1:+42137277 Msa0548300:CDS
ATTATGGCTGGAAAAGGTGA+AGG 0.630083 4_1:+42137187 None:intergenic
GGATGGAAATCAACTTGGAG+AGG 0.632319 4_1:+42139746 Msa0548300:CDS
TGGTACAACAATTAAGAATG+CGG 0.632992 4_1:+42138402 Msa0548300:CDS
TAAGCAATAGCAGCAGCAGT+AGG 0.633763 4_1:-42138523 None:intergenic
AACACCTGCTCCTTCTTGGT+AGG 0.636528 4_1:-42139264 None:intergenic
AGACAAGATGAAGGAGCTGG+AGG 0.638733 4_1:+42139782 Msa0548300:CDS
GGAACACCCCTTGGAGCAGG+AGG 0.642035 4_1:-42139396 None:intergenic
GAAGATGAAGGAGATTGCAG+AGG 0.642514 4_1:+42138372 Msa0548300:CDS
GGTGCTGGTGGTGATGCTGG+AGG 0.646421 4_1:+42139840 Msa0548300:CDS
TCAAAGCAGACAGTGATCTG+AGG 0.646469 4_1:-42139417 None:intergenic
GGATATCAGTGGAAATCCGA+GGG 0.650254 4_1:+42138756 Msa0548300:CDS
AGGATATCAGTGGAAATCCG+AGG 0.651537 4_1:+42138755 Msa0548300:CDS
TACACCTGAATCAACACACC+AGG 0.652080 4_1:-42139309 None:intergenic
AATTGAAGATGCTATTGAGG+GGG 0.652473 4_1:+42139710 Msa0548300:CDS
ATTACAATCACCAATGACAA+GGG 0.655670 4_1:+42139504 Msa0548300:CDS
GGCATACCTCCTGCTCCAAG+GGG 0.657082 4_1:+42139390 Msa0548300:CDS
GATTACAATCACCAATGACA+AGG 0.657271 4_1:+42139503 Msa0548300:CDS
GGAGGAGCCATGGATGAGGA+TGG 0.657990 4_1:+42139858 Msa0548300:CDS
CCTGAATCAACACACCAGGT+TGG 0.659727 4_1:-42139305 None:intergenic
TTCACAACGTCAAGCTACCA+AGG 0.660328 4_1:+42138453 Msa0548300:CDS
CTGCTTGTGAGAGAGCCAAG+AGG 0.662258 4_1:+42138797 Msa0548300:CDS
ACCAGGTTGGTTATCAGAGT+AGG 0.664582 4_1:-42139292 None:intergenic
TGCTGACAAACCAATGATTG+TGG 0.668634 4_1:+42138288 Msa0548300:CDS
AGGATGTATCAAGGTGCTGG+TGG 0.672624 4_1:+42139828 Msa0548300:CDS
AAATTGAAGATGCTATTGAG+GGG 0.691800 4_1:+42139709 Msa0548300:CDS
GATTGTGGTGAACTATAAGG+CGG 0.709553 4_1:+42138303 Msa0548300:CDS
ATTGAGTCCAGAAATGACAC+CGG 0.722092 4_1:-42138479 None:intergenic
GCTGGTGATACACATTTGGG+AGG 0.733697 4_1:+42138670 Msa0548300:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCAAAAATTTACAATAATT+AGG - chr4_1:42137539-42137558 None:intergenic 10.0%
!! TTATTAAAAAAACAGATTAC+TGG - chr4_1:42137628-42137647 None:intergenic 15.0%
!!! AAATTCACTTTTTTATCTTT+TGG - chr4_1:42137781-42137800 None:intergenic 15.0%
!!! GTTATTTTTATGTAGTAAAT+AGG + chr4_1:42138063-42138082 Msa0548300:intron 15.0%
!!! TAAATTTTTGATCTTCATCT+TGG + chr4_1:42137547-42137566 Msa0548300:intron 20.0%
!!! ATTGTTGTTGATAATAACTT+GGG + chr4_1:42137704-42137723 Msa0548300:intron 20.0%
!! TGGAACAGAATAAAATTAAA+AGG - chr4_1:42137761-42137780 None:intergenic 20.0%
!!! ATTGATTTGGTTATATGATT+AGG + chr4_1:42137901-42137920 Msa0548300:intron 20.0%
!!! TTAAGTTTTAGTATGTAAGT+AGG + chr4_1:42137944-42137963 Msa0548300:intron 20.0%
!! AAACAATGAATCAATTTCAA+TGG - chr4_1:42138848-42138867 None:intergenic 20.0%
! ACTGAAACAGAATAAAACTT+CGG - chr4_1:42137445-42137464 None:intergenic 25.0%
!! TTTGAAATTTCTAATGCATG+AGG + chr4_1:42137504-42137523 Msa0548300:intron 25.0%
!!! GATTGTTGTTGATAATAACT+TGG + chr4_1:42137703-42137722 Msa0548300:intron 25.0%
!! TTTTGCATTGTTTATACGAT+TGG + chr4_1:42137828-42137847 Msa0548300:intron 25.0%
!!! ATTGGTTTGGTTATATGATT+AGG + chr4_1:42137846-42137865 Msa0548300:intron 25.0%
! CATTGTTTATACGATTGATT+TGG + chr4_1:42137888-42137907 Msa0548300:intron 25.0%
!! AGAAGTAAGATTGTTGATAA+TGG + chr4_1:42138107-42138126 Msa0548300:intron 25.0%
!! AATAAGAACATTCTTTTCAC+CGG - chr4_1:42138575-42138594 None:intergenic 25.0%
!!! AATGTTCTTATTTTCGATCT+TGG + chr4_1:42138583-42138602 Msa0548300:CDS 25.0%
!!! TGAAGATTTTGATAACAGAA+TGG + chr4_1:42138693-42138712 Msa0548300:CDS 25.0%
! TCTTGAATTCTTGAACAAAA+TGG - chr4_1:42138723-42138742 None:intergenic 25.0%
!! ATTTTGTTCAAGAATTCAAG+AGG + chr4_1:42138722-42138741 Msa0548300:CDS 25.0%
!! TGAAATTGATTCATTGTTTG+AGG + chr4_1:42138849-42138868 Msa0548300:CDS 25.0%
!! GAAATTGATTCATTGTTTGA+GGG + chr4_1:42138850-42138869 Msa0548300:CDS 25.0%
!! TGAGACATTTAAAATACCAT+TGG - chr4_1:42139451-42139470 None:intergenic 25.0%
! AAAATTGAAGATGCTATTGA+GGG + chr4_1:42139708-42139727 Msa0548300:CDS 25.0%
!! AATCAACTTCCTCAATTTTT+GGG - chr4_1:42139914-42139933 None:intergenic 25.0%
!! TAATCAACTTCCTCAATTTT+TGG - chr4_1:42139915-42139934 None:intergenic 25.0%
!! TTATGTTGCTTTCACTGATA+CGG + chr4_1:42137318-42137337 Msa0548300:CDS 30.0%
TTGATCTTCATCTTGGTTAT+AGG + chr4_1:42137554-42137573 Msa0548300:intron 30.0%
TGGTTATAGGATTAGATGAT+TGG + chr4_1:42137567-42137586 Msa0548300:intron 30.0%
GGTTATAGGATTAGATGATT+GGG + chr4_1:42137568-42137587 Msa0548300:intron 30.0%
! CATTGTTTATACGATTGGTT+TGG + chr4_1:42137833-42137852 Msa0548300:intron 30.0%
! AGAAGTAAGATTGTTGATGA+TGG + chr4_1:42137988-42138007 Msa0548300:intron 30.0%
!!! TGTTGATAATGGTTTTGCAT+TGG + chr4_1:42138118-42138137 Msa0548300:intron 30.0%
TTATAGTTCACCACAATCAT+TGG - chr4_1:42138301-42138320 None:intergenic 30.0%
AATGATTGTGGTGAACTATA+AGG + chr4_1:42138300-42138319 Msa0548300:CDS 30.0%
TGGTACAACAATTAAGAATG+CGG + chr4_1:42138402-42138421 Msa0548300:CDS 30.0%
TGTTTCACTTCTTACTATTG+AGG + chr4_1:42138621-42138640 Msa0548300:CDS 30.0%
!!! TATTGAGGAAGGTATTTTTG+AGG + chr4_1:42138636-42138655 Msa0548300:CDS 30.0%
ATTCAAGAGGAAGAATAAGA+AGG + chr4_1:42138735-42138754 Msa0548300:CDS 30.0%
AAGAATAAGAAGGATATCAG+TGG + chr4_1:42138745-42138764 Msa0548300:CDS 30.0%
CAATGAATCAATTTCAATGG+TGG - chr4_1:42138845-42138864 None:intergenic 30.0%
! GGATTTGTTTAGAAAGTGTA+TGG + chr4_1:42138924-42138943 Msa0548300:CDS 30.0%
! CTTTTGCAAGATTTCTTCAA+TGG + chr4_1:42139048-42139067 Msa0548300:CDS 30.0%
!! TATTTTAAATGTCTCAGCAG+AGG + chr4_1:42139455-42139474 Msa0548300:CDS 30.0%
ATTACAATCACCAATGACAA+GGG + chr4_1:42139504-42139523 Msa0548300:CDS 30.0%
!! ATAGTTTTCAAGCGCATTTT+TGG - chr4_1:42139619-42139638 None:intergenic 30.0%
GAAAATTGAAGATGCTATTG+AGG + chr4_1:42139707-42139726 Msa0548300:CDS 30.0%
AAATTGAAGATGCTATTGAG+GGG + chr4_1:42139709-42139728 Msa0548300:CDS 30.0%
TGAATTTGAAGACAAGATGA+AGG + chr4_1:42139773-42139792 Msa0548300:CDS 30.0%
GGAACAACATATTCATGTGT+TGG + chr4_1:42137232-42137251 Msa0548300:CDS 35.0%
! CATATTCATGTGTTGGTGTT+TGG + chr4_1:42137239-42137258 Msa0548300:CDS 35.0%
TCAGTGAAAGCAACATAAGA+AGG - chr4_1:42137316-42137335 None:intergenic 35.0%
! AACCCTACTAACACTGTTTT+CGG + chr4_1:42137382-42137401 Msa0548300:CDS 35.0%
TACCGAAAACAGTGTTAGTA+GGG - chr4_1:42137387-42137406 None:intergenic 35.0%
TTACCGAAAACAGTGTTAGT+AGG - chr4_1:42137388-42137407 None:intergenic 35.0%
TCGGAAAATCAAGCATTAAG+AGG - chr4_1:42137426-42137445 None:intergenic 35.0%
CTGATTGTGTTTGCTTGATT+TGG + chr4_1:42138168-42138187 Msa0548300:intron 35.0%
CTTCATCTTCATAAGAACCA+TGG - chr4_1:42138362-42138381 None:intergenic 35.0%
CATGGTTCTTATGAAGATGA+AGG + chr4_1:42138360-42138379 Msa0548300:CDS 35.0%
TGTGAATCATTGAAGTAAGC+AGG - chr4_1:42138439-42138458 None:intergenic 35.0%
GATAAGAAAGCTACAAGTAC+CGG + chr4_1:42138553-42138572 Msa0548300:CDS 35.0%
!!! GTTCTTATTTTCGATCTTGG+TGG + chr4_1:42138586-42138605 Msa0548300:CDS 35.0%
TCACTTCTTACTATTGAGGA+AGG + chr4_1:42138625-42138644 Msa0548300:CDS 35.0%
!! GTTGATGATGTTGTTCTAGT+TGG + chr4_1:42138994-42139013 Msa0548300:CDS 35.0%
TGCAAAAGTTGTTGAACCTT+CGG - chr4_1:42139036-42139055 None:intergenic 35.0%
GCAAGATTTCTTCAATGGAA+AGG + chr4_1:42139053-42139072 Msa0548300:CDS 35.0%
ACTCGTGATAACAATCTGTT+GGG + chr4_1:42139351-42139370 Msa0548300:CDS 35.0%
GATTACAATCACCAATGACA+AGG + chr4_1:42139503-42139522 Msa0548300:CDS 35.0%
CATCTTCTCAATTTCCTCTT+TGG - chr4_1:42139538-42139557 None:intergenic 35.0%
CAAAGAGGAAATTGAGAAGA+TGG + chr4_1:42139536-42139555 Msa0548300:CDS 35.0%
AAAACTATGCCTACAACATG+AGG + chr4_1:42139631-42139650 Msa0548300:CDS 35.0%
AATTGAAGATGCTATTGAGG+GGG + chr4_1:42139710-42139729 Msa0548300:CDS 35.0%
GATACATCCTTGCAATGATA+GGG - chr4_1:42139818-42139837 None:intergenic 35.0%
TGATACATCCTTGCAATGAT+AGG - chr4_1:42139819-42139838 None:intergenic 35.0%
ATCATTGCAAGGATGTATCA+AGG + chr4_1:42139819-42139838 Msa0548300:CDS 35.0%
TGAGATCATAGCAAACGATC+AGG + chr4_1:42137276-42137295 Msa0548300:CDS 40.0%
GAGATCATAGCAAACGATCA+GGG + chr4_1:42137277-42137296 Msa0548300:CDS 40.0%
ACTGATACGGAACGTTTGAT+CGG + chr4_1:42137331-42137350 Msa0548300:CDS 40.0%
TAGTAGGGTTCATAGCAACT+TGG - chr4_1:42137372-42137391 None:intergenic 40.0%
!! TGTTGATGATGGTCTTGCAT+TGG + chr4_1:42137999-42138018 Msa0548300:intron 40.0%
ATCCTCAAGTGATTGTGCAT+AGG + chr4_1:42138041-42138060 Msa0548300:intron 40.0%
AACCTATGCACAATCACTTG+AGG - chr4_1:42138046-42138065 None:intergenic 40.0%
TCAGATGCCAAGCGTTTAAT+TGG + chr4_1:42138196-42138215 Msa0548300:intron 40.0%
ATGCCAAGCGTTTAATTGGT+AGG + chr4_1:42138200-42138219 Msa0548300:CDS 40.0%
TACAGAGCGACATGAAATTG+TGG + chr4_1:42138242-42138261 Msa0548300:CDS 40.0%
CATGAAATTGTGGCCATTCA+AGG + chr4_1:42138252-42138271 Msa0548300:CDS 40.0%
!! CAATCATTGGTTTGTCAGCA+GGG - chr4_1:42138288-42138307 None:intergenic 40.0%
!! ACAATCATTGGTTTGTCAGC+AGG - chr4_1:42138289-42138308 None:intergenic 40.0%
TGCTGACAAACCAATGATTG+TGG + chr4_1:42138288-42138307 Msa0548300:CDS 40.0%
GATTGTGGTGAACTATAAGG+CGG + chr4_1:42138303-42138322 Msa0548300:CDS 40.0%
TGCTGAAGAGATATCTTCCA+TGG + chr4_1:42138342-42138361 Msa0548300:CDS 40.0%
ATTGAGTCCAGAAATGACAC+CGG - chr4_1:42138482-42138501 None:intergenic 40.0%
!! CTTATTTTCGATCTTGGTGG+TGG + chr4_1:42138589-42138608 Msa0548300:CDS 40.0%
TACTGCTGGTGATACACATT+TGG + chr4_1:42138666-42138685 Msa0548300:CDS 40.0%
ACTGCTGGTGATACACATTT+GGG + chr4_1:42138667-42138686 Msa0548300:CDS 40.0%
CATGTTGAGCTCTTCAAAAC+GGG - chr4_1:42138905-42138924 None:intergenic 40.0%
CCATGTTGAGCTCTTCAAAA+CGG - chr4_1:42138906-42138925 None:intergenic 40.0%
!! AGCCTGTTGAGAAGTGTTTA+AGG + chr4_1:42138947-42138966 Msa0548300:CDS 40.0%
!! GCCTGTTGAGAAGTGTTTAA+GGG + chr4_1:42138948-42138967 Msa0548300:CDS 40.0%
TCCCTTAAACACTTCTCAAC+AGG - chr4_1:42138952-42138971 None:intergenic 40.0%
GTGTTTAAGGGATGCTAAGA+TGG + chr4_1:42138960-42138979 Msa0548300:CDS 40.0%
! GATGATGTTGTTCTAGTTGG+TGG + chr4_1:42138997-42139016 Msa0548300:CDS 40.0%
!! TTCTAGTTGGTGGATCTACT+AGG + chr4_1:42139007-42139026 Msa0548300:CDS 40.0%
! TGAGGGAAATGAGAAAGTAC+AGG + chr4_1:42139149-42139168 Msa0548300:CDS 40.0%
GTAGGAATGGTTGTGTTTCT+CGG - chr4_1:42139249-42139268 None:intergenic 40.0%
!! GTGTTGATTCAGGTGTATGA+AGG + chr4_1:42139315-42139334 Msa0548300:CDS 40.0%
TGTATGAAGGCGAAAGAACA+AGG + chr4_1:42139328-42139347 Msa0548300:CDS 40.0%
GACTCGTGATAACAATCTGT+TGG + chr4_1:42139350-42139369 Msa0548300:CDS 40.0%
TGCTTTGACATTGATGCCAA+TGG + chr4_1:42139432-42139451 Msa0548300:CDS 40.0%
! AGCTGAAAAGTACAAGTCTG+AGG + chr4_1:42139566-42139585 Msa0548300:CDS 40.0%
AAAGTACAAGTCTGAGGATG+AGG + chr4_1:42139572-42139591 Msa0548300:CDS 40.0%
GATTGTGTTCCTCATGTTGT+AGG - chr4_1:42139643-42139662 None:intergenic 40.0%
TGGTTGGATGGAAATCAACT+TGG + chr4_1:42139741-42139760 Msa0548300:CDS 40.0%
TTTGCAACCCTATCATTGCA+AGG + chr4_1:42139808-42139827 Msa0548300:CDS 40.0%
ACCTGCGATTGGAATCGATT+TGG + chr4_1:42137210-42137229 Msa0548300:CDS 45.0%
! CCTGCGATTGGAATCGATTT+GGG + chr4_1:42137211-42137230 Msa0548300:CDS 45.0%
TCGCCTACCAATTAAACGCT+TGG - chr4_1:42138206-42138225 None:intergenic 45.0%
TAGGCGAATCAGCGATGTTT+CGG + chr4_1:42138219-42138238 Msa0548300:CDS 45.0%
GAAGATGAAGGAGATTGCAG+AGG + chr4_1:42138372-42138391 Msa0548300:CDS 45.0%
GAGATTGCAGAGGCTTATCT+TGG + chr4_1:42138382-42138401 Msa0548300:CDS 45.0%
TTCACAACGTCAAGCTACCA+AGG + chr4_1:42138453-42138472 Msa0548300:CDS 45.0%
TAAGCAATAGCAGCAGCAGT+AGG - chr4_1:42138526-42138545 None:intergenic 45.0%
ACTGCTGCTGCTATTGCTTA+TGG + chr4_1:42138526-42138545 Msa0548300:CDS 45.0%
! TTTGAGGTGAAAGCTACTGC+TGG + chr4_1:42138652-42138671 Msa0548300:CDS 45.0%
AGGATATCAGTGGAAATCCG+AGG + chr4_1:42138755-42138774 Msa0548300:CDS 45.0%
GGATATCAGTGGAAATCCGA+GGG + chr4_1:42138756-42138775 Msa0548300:CDS 45.0%
GGTTTGAGCAGTAGAGGAAA+GGG - chr4_1:42138824-42138843 None:intergenic 45.0%
TGGTTTGAGCAGTAGAGGAA+AGG - chr4_1:42138825-42138844 None:intergenic 45.0%
AATGGTGGTTTGAGCAGTAG+AGG - chr4_1:42138830-42138849 None:intergenic 45.0%
!!! CCGTTTTGAAGAGCTCAACA+TGG + chr4_1:42138903-42138922 Msa0548300:CDS 45.0%
TGGATCTACTAGGATTCCGA+AGG + chr4_1:42139017-42139036 Msa0548300:CDS 45.0%
CTGATGAAGCCGTTGCATAT+GGG + chr4_1:42139097-42139116 Msa0548300:CDS 45.0%
TGATGAAGCCGTTGCATATG+GGG + chr4_1:42139098-42139117 Msa0548300:CDS 45.0%
!!! GTCCAAGCTGCTATTTTGAG+TGG + chr4_1:42139126-42139145 Msa0548300:CDS 45.0%
CACCACTCAAAATAGCAGCT+TGG - chr4_1:42139131-42139150 None:intergenic 45.0%
!!! AGCTGCTATTTTGAGTGGTG+AGG + chr4_1:42139131-42139150 Msa0548300:CDS 45.0%
!!! GCTGCTATTTTGAGTGGTGA+GGG + chr4_1:42139132-42139151 Msa0548300:CDS 45.0%
AGTACAGGATCTGTTGTTGC+TGG + chr4_1:42139164-42139183 Msa0548300:CDS 45.0%
GATGTTACTCCTCTTTCCCT+TGG + chr4_1:42139186-42139205 Msa0548300:CDS 45.0%
TACTCCTCTTTCCCTTGGTT+TGG + chr4_1:42139191-42139210 Msa0548300:CDS 45.0%
CACAACCATTCCTACCAAGA+AGG + chr4_1:42139254-42139273 Msa0548300:CDS 45.0%
ACCTACTCTGATAACCAACC+TGG + chr4_1:42139291-42139310 Msa0548300:CDS 45.0%
!! ACCAGGTTGGTTATCAGAGT+AGG - chr4_1:42139295-42139314 None:intergenic 45.0%
TACACCTGAATCAACACACC+AGG - chr4_1:42139312-42139331 None:intergenic 45.0%
!! GTTGGGCAAGTTTGAGTTGT+CGG + chr4_1:42139368-42139387 Msa0548300:CDS 45.0%
!! TTGGGCAAGTTTGAGTTGTC+GGG + chr4_1:42139369-42139388 Msa0548300:CDS 45.0%
TCAAAGCAGACAGTGATCTG+AGG - chr4_1:42139420-42139439 None:intergenic 45.0%
CAATCACCAATGACAAGGGT+AGG + chr4_1:42139508-42139527 Msa0548300:CDS 45.0%
GGAAATTGAGAAGATGGTGC+AGG + chr4_1:42139542-42139561 Msa0548300:CDS 45.0%
! GGATGAGGAGCACAAAAAGA+AGG + chr4_1:42139587-42139606 Msa0548300:CDS 45.0%
! TGAGGAGCACAAAAAGAAGG+TGG + chr4_1:42139590-42139609 Msa0548300:CDS 45.0%
CTATTGAGGGGGCTATTCAA+TGG + chr4_1:42139721-42139740 Msa0548300:CDS 45.0%
GGATGGAAATCAACTTGGAG+AGG + chr4_1:42139746-42139765 Msa0548300:CDS 45.0%
TGAAGACAAGATGAAGGAGC+TGG + chr4_1:42139779-42139798 Msa0548300:CDS 45.0%
AAAGGTGAAGGACCTGCGAT+TGG + chr4_1:42137199-42137218 Msa0548300:CDS 50.0%
! CCCAAATCGATTCCAATCGC+AGG - chr4_1:42137214-42137233 None:intergenic 50.0%
TGGCCATTCAAGGTCATTCC+TGG + chr4_1:42138262-42138281 Msa0548300:CDS 50.0%
TGTGGTGAACTATAAGGCGG+AGG + chr4_1:42138306-42138325 Msa0548300:CDS 50.0%
AGAAATGACACCGGCATCCT+TGG - chr4_1:42138473-42138492 None:intergenic 50.0%
!! AAGGATGCCGGTGTCATTTC+TGG + chr4_1:42138472-42138491 Msa0548300:CDS 50.0%
GCTGGTGATACACATTTGGG+AGG + chr4_1:42138670-42138689 Msa0548300:CDS 50.0%
CTCAATCTTCTCAGAGCCCT+CGG - chr4_1:42138775-42138794 None:intergenic 50.0%
AGTAGAGGAAAGGGTCCTCT+TGG - chr4_1:42138815-42138834 None:intergenic 50.0%
CCATATGCAACGGCTTCATC+AGG - chr4_1:42139099-42139118 None:intergenic 50.0%
CCTGATGAAGCCGTTGCATA+TGG + chr4_1:42139096-42139115 Msa0548300:CDS 50.0%
GTCTCCAAACCAAGGGAAAG+AGG - chr4_1:42139198-42139217 None:intergenic 50.0%
TCCTGCAGTCTCCAAACCAA+GGG - chr4_1:42139205-42139224 None:intergenic 50.0%
CTGCTCCTTCTTGGTAGGAA+TGG - chr4_1:42139262-42139281 None:intergenic 50.0%
CATTCCTACCAAGAAGGAGC+AGG + chr4_1:42139260-42139279 Msa0548300:CDS 50.0%
AACACCTGCTCCTTCTTGGT+AGG - chr4_1:42139267-42139286 None:intergenic 50.0%
CGAAAACACCTGCTCCTTCT+TGG - chr4_1:42139271-42139290 None:intergenic 50.0%
CCTGAATCAACACACCAGGT+TGG - chr4_1:42139308-42139327 None:intergenic 50.0%
!! CCAACCTGGTGTGTTGATTC+AGG + chr4_1:42139305-42139324 Msa0548300:CDS 50.0%
TCAGCAGAGGACAAGACTAC+AGG + chr4_1:42139468-42139487 Msa0548300:CDS 50.0%
CAGCAGAGGACAAGACTACA+GGG + chr4_1:42139469-42139488 Msa0548300:CDS 50.0%
GGAAAGCCTACCCTTGTCAT+TGG - chr4_1:42139517-42139536 None:intergenic 50.0%
CAAGGGTAGGCTTTCCAAAG+AGG + chr4_1:42139521-42139540 Msa0548300:CDS 50.0%
GGAGCACAAAAAGAAGGTGG+AGG + chr4_1:42139593-42139612 Msa0548300:CDS 50.0%
TGAGGGGGCTATTCAATGGT+TGG + chr4_1:42139725-42139744 Msa0548300:CDS 50.0%
GGGGCTATTCAATGGTTGGA+TGG + chr4_1:42139729-42139748 Msa0548300:CDS 50.0%
AGACAAGATGAAGGAGCTGG+AGG + chr4_1:42139782-42139801 Msa0548300:CDS 50.0%
GACAAGATGAAGGAGCTGGA+GGG + chr4_1:42139783-42139802 Msa0548300:CDS 50.0%
GCAAGGATGTATCAAGGTGC+TGG + chr4_1:42139825-42139844 Msa0548300:CDS 50.0%
!! AGGATGTATCAAGGTGCTGG+TGG + chr4_1:42139828-42139847 Msa0548300:CDS 50.0%
!! TGGTGCTGGCCCAAAAATTG+AGG + chr4_1:42139902-42139921 Msa0548300:CDS 50.0%
GGGCCAGGAATGACCTTGAA+TGG - chr4_1:42138268-42138287 None:intergenic 55.0%
!! ATTGGTTTGTCAGCAGGGCC+AGG - chr4_1:42138283-42138302 None:intergenic 55.0%
CGTCAAGCTACCAAGGATGC+CGG + chr4_1:42138460-42138479 Msa0548300:CDS 55.0%
CTGCTTGTGAGAGAGCCAAG+AGG + chr4_1:42138797-42138816 Msa0548300:CDS 55.0%
GACTGCTGCCCCATATGCAA+CGG - chr4_1:42139109-42139128 None:intergenic 55.0%
!! TCCCTTGGTTTGGAGACTGC+AGG + chr4_1:42139201-42139220 Msa0548300:CDS 55.0%
CTCCTGCAGTCTCCAAACCA+AGG - chr4_1:42139206-42139225 None:intergenic 55.0%
!! CTTGGTTTGGAGACTGCAGG+AGG + chr4_1:42139204-42139223 Msa0548300:CDS 55.0%
GTGATCTGAGGAACACCCCT+TGG - chr4_1:42139408-42139427 None:intergenic 55.0%
AGCAGGACCATCCTCATCCA+TGG - chr4_1:42139868-42139887 None:intergenic 55.0%
GGGCATACCTCCTGCTCCAA+GGG + chr4_1:42139389-42139408 Msa0548300:CDS 60.0%
GGCATACCTCCTGCTCCAAG+GGG + chr4_1:42139390-42139409 Msa0548300:CDS 60.0%
TGAGGAACACCCCTTGGAGC+AGG - chr4_1:42139402-42139421 None:intergenic 60.0%
!! CAAGGTGCTGGTGGTGATGC+TGG + chr4_1:42139837-42139856 Msa0548300:CDS 60.0%
! TGGTGATGCTGGAGGAGCCA+TGG + chr4_1:42139848-42139867 Msa0548300:CDS 60.0%
TGCTGGAGGAGCCATGGATG+AGG + chr4_1:42139854-42139873 Msa0548300:CDS 60.0%
GGAGGAGCCATGGATGAGGA+TGG + chr4_1:42139858-42139877 Msa0548300:CDS 60.0%
GATGAGGATGGTCCTGCTGC+TGG + chr4_1:42139870-42139889 Msa0548300:CDS 60.0%
CGGGCATACCTCCTGCTCCA+AGG + chr4_1:42139388-42139407 Msa0548300:CDS 65.0%
GGAACACCCCTTGGAGCAGG+AGG - chr4_1:42139399-42139418 None:intergenic 65.0%
!! GGTGCTGGTGGTGATGCTGG+AGG + chr4_1:42139840-42139859 Msa0548300:CDS 65.0%
GATGGTCCTGCTGCTGGCAG+TGG + chr4_1:42139876-42139895 Msa0548300:CDS 65.0%
CCACTTCCACTGCCAGCAGC+AGG - chr4_1:42139885-42139904 None:intergenic 65.0%
CCTGCTGCTGGCAGTGGAAG+TGG + chr4_1:42139882-42139901 Msa0548300:CDS 65.0%
GCTGGCAGTGGAAGTGGTGC+TGG + chr4_1:42139888-42139907 Msa0548300:CDS 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 42137190 42139935 42137190 ID=Msa0548300;Name=Msa0548300
chr4_1 mRNA 42137190 42139935 42137190 ID=Msa0548300-mRNA-1;Parent=Msa0548300;Name=Msa0548300-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|0|0|1|1|1|2|0|649
chr4_1 exon 42137190 42137403 42137190 ID=Msa0548300-mRNA-1:exon:10888;Parent=Msa0548300-mRNA-1
chr4_1 exon 42138200 42139935 42138200 ID=Msa0548300-mRNA-1:exon:10889;Parent=Msa0548300-mRNA-1
chr4_1 CDS 42137190 42137403 42137190 ID=Msa0548300-mRNA-1:cds;Parent=Msa0548300-mRNA-1
chr4_1 CDS 42138200 42139935 42138200 ID=Msa0548300-mRNA-1:cds;Parent=Msa0548300-mRNA-1
Gene Sequence

>Msa0548300

ATGGCTGGAAAAGGTGAAGGACCTGCGATTGGAATCGATTTGGGAACAACATATTCATGTGTTGGTGTTTGGCAACATGATCGTGTTGAGATCATAGCAAACGATCAGGGTAACAGAACTACTCCTTCTTATGTTGCTTTCACTGATACGGAACGTTTGATCGGTGATGCTGCAAAGAACCAAGTTGCTATGAACCCTACTAACACTGTTTTCGATGCCAAGCGTTTAATTGGTAGGCGAATCAGCGATGTTTCGGTACAGAGCGACATGAAATTGTGGCCATTCAAGGTCATTCCTGGCCCTGCTGACAAACCAATGATTGTGGTGAACTATAAGGCGGAGGAGAAACAATTTTCTGCTGAAGAGATATCTTCCATGGTTCTTATGAAGATGAAGGAGATTGCAGAGGCTTATCTTGGTACAACAATTAAGAATGCGGTTGTAACTGTGCCTGCTTACTTCAATGATTCACAACGTCAAGCTACCAAGGATGCCGGTGTCATTTCTGGACTCAATGTAATGAGAATCATTAATGAACCTACTGCTGCTGCTATTGCTTATGGTCTTGATAAGAAAGCTACAAGTACCGGTGAAAAGAATGTTCTTATTTTCGATCTTGGTGGTGGAACTTTTGATGTTTCACTTCTTACTATTGAGGAAGGTATTTTTGAGGTGAAAGCTACTGCTGGTGATACACATTTGGGAGGTGAAGATTTTGATAACAGAATGGTGAACCATTTTGTTCAAGAATTCAAGAGGAAGAATAAGAAGGATATCAGTGGAAATCCGAGGGCTCTGAGAAGATTGAGAACTGCTTGTGAGAGAGCCAAGAGGACCCTTTCCTCTACTGCTCAAACCACCATTGAAATTGATTCATTGTTTGAGGGTATTGACTTTTACACTACTATTACTCGTGCCCGTTTTGAAGAGCTCAACATGGATTTGTTTAGAAAGTGTATGGAGCCTGTTGAGAAGTGTTTAAGGGATGCTAAGATGGATAAGAACAGTGTTGATGATGTTGTTCTAGTTGGTGGATCTACTAGGATTCCGAAGGTTCAACAACTTTTGCAAGATTTCTTCAATGGAAAGGAGCTATGTAAGAGCATCAATCCTGATGAAGCCGTTGCATATGGGGCAGCAGTCCAAGCTGCTATTTTGAGTGGTGAGGGAAATGAGAAAGTACAGGATCTGTTGTTGCTGGATGTTACTCCTCTTTCCCTTGGTTTGGAGACTGCAGGAGGTGTCATGACTGTGTTGATTCCGAGAAACACAACCATTCCTACCAAGAAGGAGCAGGTGTTTTCGACCTACTCTGATAACCAACCTGGTGTGTTGATTCAGGTGTATGAAGGCGAAAGAACAAGGACTCGTGATAACAATCTGTTGGGCAAGTTTGAGTTGTCGGGCATACCTCCTGCTCCAAGGGGTGTTCCTCAGATCACTGTCTGCTTTGACATTGATGCCAATGGTATTTTAAATGTCTCAGCAGAGGACAAGACTACAGGGCAGAAAAATAAGATTACAATCACCAATGACAAGGGTAGGCTTTCCAAAGAGGAAATTGAGAAGATGGTGCAGGAAGCTGAAAAGTACAAGTCTGAGGATGAGGAGCACAAAAAGAAGGTGGAGGCCAAAAATGCGCTTGAAAACTATGCCTACAACATGAGGAACACAATCAAAGACGACAAGATTGCTTCGAAGTTGTCTGCTGATGATAAGAAGAAAATTGAAGATGCTATTGAGGGGGCTATTCAATGGTTGGATGGAAATCAACTTGGAGAGGCTGATGAATTTGAAGACAAGATGAAGGAGCTGGAGGGCATTTGCAACCCTATCATTGCAAGGATGTATCAAGGTGCTGGTGGTGATGCTGGAGGAGCCATGGATGAGGATGGTCCTGCTGCTGGCAGTGGAAGTGGTGCTGGCCCAAAAATTGAGGAAGTTGATTAA
Protein sequence

>Msa0548300

MAGKGEGPAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRISDVSVQSDMKLWPFKVIPGPADKPMIVVNYKAEEKQFSAEEISSMVLMKMKEIAEAYLGTTIKNAVVTVPAYFNDSQRQATKDAGVISGLNVMRIINEPTAAAIAYGLDKKATSTGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTHLGGEDFDNRMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLFEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKNSVDDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAGGVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFELSGIPPAPRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKMVQEAEKYKSEDEEHKKKVEAKNALENYAYNMRNTIKDDKIASKLSADDKKKIEDAIEGAIQWLDGNQLGEADEFEDKMKELEGICNPIIARMYQGAGGDAGGAMDEDGPAAGSGSGAGPKIEEVD*