AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0644230

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0644230 MtrunA17_Chr4g0031301 99.384 649 4 0 1 649 1 649 0.0 1325
Msa0644230 MtrunA17_Chr4g0031321 99.230 649 5 0 1 649 1 649 0.0 1325
Msa0644230 MtrunA17_Chr7g0223291 92.934 651 42 3 1 649 1 649 0.0 1239
Msa0644230 MtrunA17_Chr7g0262821 92.296 649 50 0 1 649 1 649 0.0 1238
Msa0644230 MtrunA17_Chr7g0223181 92.627 651 43 2 1 649 1 648 0.0 1230
Msa0644230 MtrunA17_Chr4g0073741 87.768 654 74 3 1 649 1 653 0.0 1161
Msa0644230 MtrunA17_Chr6g0462681 66.992 615 202 1 1 615 1 614 0.0 850
Msa0644230 MtrunA17_Chr2g0284681 68.046 604 173 3 9 606 8 597 0.0 839
Msa0644230 MtrunA17_Chr7g0223751 66.345 621 204 4 1 619 1 618 0.0 838
Msa0644230 MtrunA17_Chr6g0462601 65.909 616 209 1 1 616 1 615 0.0 834
Msa0644230 MtrunA17_Chr7g0223711 65.056 621 213 3 1 619 1 619 0.0 828
Msa0644230 MtrunA17_Chr7g0221661 65.366 615 209 3 1 613 1 613 0.0 817
Msa0644230 MtrunA17_Chr2g0298851 65.590 619 206 6 1 615 1 616 0.0 813
Msa0644230 MtrunA17_Chr5g0427901 64.172 628 210 4 1 615 1 626 0.0 812
Msa0644230 MtrunA17_Chr7g0223741 66.164 597 198 3 1 595 1 595 0.0 812
Msa0644230 MtrunA17_Chr8g0350731 64.480 625 217 4 1 623 29 650 0.0 807
Msa0644230 MtrunA17_Chr2g0293581 64.927 613 211 3 6 615 7 618 0.0 804
Msa0644230 MtrunA17_Chr8g0388761 65.635 614 205 4 7 618 36 645 0.0 804
Msa0644230 MtrunA17_Chr8g0388671 65.635 614 205 4 7 618 36 645 0.0 802
Msa0644230 MtrunA17_Chr7g0223801 63.371 617 220 5 1 615 1 613 0.0 800
Msa0644230 MtrunA17_Chr7g0223731 64.803 608 209 4 1 606 1 605 0.0 798
Msa0644230 MtrunA17_Chr7g0233311 63.430 618 222 3 1 615 1 617 0.0 798
Msa0644230 MtrunA17_Chr7g0223621 64.098 610 214 4 1 608 1 607 0.0 791
Msa0644230 MtrunA17_Chr7g0223031 63.993 611 215 4 1 609 1 608 0.0 790
Msa0644230 MtrunA17_Chr6g0462711 62.939 626 225 3 2 621 3 627 0.0 786
Msa0644230 MtrunA17_Chr7g0248131 63.578 626 218 8 1 619 1 623 0.0 785
Msa0644230 MtrunA17_Chr2g0298861 61.905 630 223 6 1 615 1 628 0.0 781
Msa0644230 MtrunA17_Chr7g0223631 63.607 621 207 6 1 619 1 604 0.0 780
Msa0644230 MtrunA17_Chr6g0462771 61.981 626 232 2 1 621 1 625 0.0 780
Msa0644230 MtrunA17_Chr7g0223781 63.089 615 221 3 1 613 1 611 0.0 779
Msa0644230 MtrunA17_Chr2g0287631 64.563 618 215 3 1 615 1 617 0.0 777
Msa0644230 MtrunA17_Chr7g0248151 63.360 625 221 6 1 620 1 622 0.0 776
Msa0644230 MtrunA17_Chr4g0040931 62.684 611 223 4 1 608 1 609 0.0 776
Msa0644230 MtrunA17_Chr7g0223701 62.843 619 216 6 1 608 1 616 0.0 775
Msa0644230 MtrunA17_Chr6g0462641 61.342 626 236 2 1 621 1 625 0.0 773
Msa0644230 MtrunA17_Chr7g0225671 63.192 614 218 7 6 615 13 622 0.0 773
Msa0644230 MtrunA17_Chr7g0223721 61.812 618 231 4 1 615 1 616 0.0 772
Msa0644230 MtrunA17_Chr7g0223851 62.682 619 224 6 1 615 10 625 0.0 764
Msa0644230 MtrunA17_Chr2g0296391 68.561 528 163 2 1 526 1 527 0.0 747
Msa0644230 MtrunA17_Chr7g0233301 60.548 621 208 6 1 617 1 588 0.0 743
Msa0644230 MtrunA17_Chr7g0223821 61.997 621 224 7 1 613 1 617 0.0 735
Msa0644230 MtrunA17_Chr7g0217971 61.296 602 204 5 1 600 1 575 0.0 734
Msa0644230 MtrunA17_Chr8g0390821 56.632 671 219 10 1 616 1 654 0.0 721
Msa0644230 MtrunA17_Chr6g0462611 64.014 553 196 2 63 615 13 562 0.0 721
Msa0644230 MtrunA17_Chr4g0012541 58.805 619 239 7 1 615 1 607 0.0 712
Msa0644230 MtrunA17_Chr7g0223831 59.411 611 211 8 1 606 1 579 0.0 701
Msa0644230 MtrunA17_Chr7g0223541 61.141 561 216 2 61 621 20 578 0.0 693
Msa0644230 MtrunA17_Chr7g0215261 57.997 619 218 7 1 614 1 582 0.0 692
Msa0644230 MtrunA17_Chr7g0228281 56.311 618 220 7 1 615 62 632 0.0 672
Msa0644230 MtrunA17_Chr2g0299921 84.576 389 58 1 4 392 1024 1410 0.0 671
Msa0644230 MtrunA17_Chr2g0299921 83.636 385 60 2 4 387 1408 1790 0.0 637
Msa0644230 MtrunA17_Chr2g0299851 83.721 387 61 1 1 387 1030 1414 0.0 667
Msa0644230 MtrunA17_Chr7g0223681 61.770 531 200 3 89 619 2 529 0.0 665
Msa0644230 MtrunA17_Chr2g0293601 63.969 519 150 3 1 517 1 484 0.0 654
Msa0644230 MtrunA17_Chr2g0299791 81.443 388 70 1 1 388 1034 1419 0.0 648
Msa0644230 MtrunA17_Chr2g0299821 72.000 450 112 3 1 436 1 450 0.0 638
Msa0644230 MtrunA17_Chr2g0299821 78.947 95 13 1 452 539 390 484 2.28e-39 151
Msa0644230 MtrunA17_Chr2g0331381 58.965 541 156 6 9 547 6 482 0.0 635
Msa0644230 MtrunA17_Chr2g0299881 77.057 401 86 3 6 402 1035 1433 0.0 627
Msa0644230 MtrunA17_Chr2g0299881 75.758 66 12 1 183 248 1414 1475 5.10e-22 101
Msa0644230 MtrunA17_Chr2g0293561 73.262 374 98 1 1 372 1 374 0.0 581
Msa0644230 MtrunA17_Chr1g0166551 50.977 614 183 8 6 615 2 501 0.0 579
Msa0644230 MtrunA17_Chr2g0279901 51.167 600 263 10 10 602 57 633 0.0 573
Msa0644230 MtrunA17_Chr6g0462861 50.159 628 277 11 1 615 1 605 0.0 568
Msa0644230 MtrunA17_Chr4g0049501 50.667 600 267 10 10 602 54 631 0.0 561
Msa0644230 MtrunA17_Chr2g0277021 47.819 642 306 13 10 641 72 694 0.0 533
Msa0644230 MtrunA17_Chr2g0293271 54.691 437 125 4 21 455 43 408 6.01e-161 468
Msa0644230 MtrunA17_Chr1g0151991 70.790 291 83 1 1 289 1 291 4.31e-149 433
Msa0644230 MtrunA17_Chr7g0245521 71.795 273 75 1 1 271 1 273 2.12e-141 412
Msa0644230 MtrunA17_Chr6g0462631 59.337 332 91 3 86 415 8 297 2.09e-124 370
Msa0644230 MtrunA17_Chr6g0462751 54.728 349 99 3 65 411 12 303 4.34e-119 357
Msa0644230 MtrunA17_Chr7g0223761 48.677 378 150 2 239 615 3 337 1.24e-117 355
Msa0644230 MtrunA17_Chr7g0245531 57.962 314 130 2 303 616 2 313 5.47e-116 349
Msa0644230 MtrunA17_Chr1g0152001 54.882 297 129 5 313 608 1 293 3.66e-101 311
Msa0644230 MtrunA17_Chr1g0188601 74.346 191 49 0 90 280 1 191 4.82e-99 301
Msa0644230 MtrunA17_Chr5g0394391 36.581 503 292 10 9 494 29 521 5.07e-97 309
Msa0644230 MtrunA17_Chr7g0223671 70.811 185 54 0 72 256 8 192 1.43e-92 285
Msa0644230 MtrunA17_Chr1g0188611 53.872 297 127 4 320 615 1 288 1.51e-86 272
Msa0644230 MtrunA17_Chr6g0462791 71.023 176 50 1 106 281 1 175 1.03e-82 258
Msa0644230 MtrunA17_Chr2g0331901 33.406 461 289 5 10 457 4 459 1.53e-82 278
Msa0644230 MtrunA17_Chr4g0004331 34.475 438 271 4 10 434 4 438 1.18e-81 275
Msa0644230 MtrunA17_Chr5g0444001 34.426 427 271 4 10 430 4 427 2.27e-78 259
Msa0644230 MtrunA17_Chr3g0098761 33.810 420 273 4 10 428 4 419 2.03e-75 257
Msa0644230 MtrunA17_Chr7g0223771 72.549 153 42 0 92 244 2 154 1.91e-74 236
Msa0644230 MtrunA17_Chr5g0394221 42.638 326 175 5 265 587 35 351 3.38e-70 232
Msa0644230 MtrunA17_Chr4g0046881 72.340 141 36 1 133 273 14 151 3.07e-68 219
Msa0644230 MtrunA17_Chr4g0064621 33.984 512 306 11 9 496 140 643 3.01e-67 233
Msa0644230 MtrunA17_Chr4g0060241 32.143 504 307 14 10 496 21 506 4.09e-67 229
Msa0644230 MtrunA17_Chr6g0462691 69.014 142 44 0 320 461 1 142 2.04e-62 204
Msa0644230 MtrunA17_Chr6g0462701 62.651 166 38 1 103 268 27 168 1.71e-61 202
Msa0644230 MtrunA17_Chr5g0442171 37.463 339 181 9 168 491 1 323 5.88e-60 203
Msa0644230 MtrunA17_Chr3g0120101 26.748 572 374 12 10 544 27 590 9.25e-59 212
Msa0644230 MtrunA17_Chr7g0223861 70.992 131 38 0 76 206 4 134 3.63e-58 192
Msa0644230 MtrunA17_Chr7g0245541 48.259 201 103 1 416 616 1 200 1.47e-53 182
Msa0644230 MtrunA17_Chr5g0394231 39.207 227 87 4 72 297 5 181 1.32e-43 154
Msa0644230 MtrunA17_Chr6g0481391 59.836 122 46 1 36 157 13 131 1.21e-39 142
Msa0644230 MtrunA17_Chr4g0046871 71.429 98 27 1 337 434 9 105 1.65e-39 140
Msa0644230 MtrunA17_Chr6g0462621 76.056 71 17 0 1 71 1 71 3.77e-32 119
Msa0644230 MtrunA17_Chr7g0223691 78.082 73 14 1 1 71 1 73 9.24e-32 117
Msa0644230 MtrunA17_Chr1g0188591 75.342 73 16 1 1 71 1 73 2.79e-30 114
Msa0644230 MtrunA17_Chr2g0284661 71.084 83 24 0 366 448 61 143 3.16e-30 115
Msa0644230 MtrunA17_Chr2g0284661 50.820 61 28 1 223 281 5 65 1.65e-11 62.4
Msa0644230 MtrunA17_Chr6g0481381 70.270 74 22 0 174 247 1 74 4.80e-30 117
Msa0644230 MtrunA17_Chr6g0481381 37.374 99 61 1 515 612 75 173 6.59e-11 62.0
Msa0644230 MtrunA17_Chr7g0223881 61.333 75 28 1 286 360 9 82 5.64e-26 102
Msa0644230 MtrunA17_Chr7g0223791 64.634 82 21 3 54 135 18 91 2.90e-24 100
Msa0644230 MtrunA17_Chr7g0223791 46.739 92 49 0 482 573 90 181 5.10e-19 85.1
Msa0644230 MtrunA17_Chr2g0299941 76.667 60 14 0 398 457 23 82 1.45e-23 95.1
Msa0644230 MtrunA17_Chr7g0221651 53.571 84 39 0 213 296 1 84 3.16e-23 94.4
Msa0644230 MtrunA17_Chr4g0064651 46.739 92 49 0 396 487 29 120 1.91e-22 93.2
Msa0644230 MtrunA17_Chr2g0299891 93.023 43 3 0 313 355 1 43 1.48e-21 89.0
Msa0644230 MtrunA17_Chr7g0223871 38.889 126 76 1 491 615 7 132 1.01e-18 83.2
Msa0644230 MtrunA17_Chr6g0462801 64.615 65 18 1 320 379 1 65 1.78e-18 80.1
Msa0644230 MtrunA17_Chr5g0442161 31.126 151 99 3 9 155 28 177 2.60e-15 74.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0644230 AT3G12580.1 92.320 651 47 2 1 649 1 650 0.0 1221
Msa0644230 AT5G02500.1 90.658 653 55 2 1 649 1 651 0.0 1213
Msa0644230 AT3G09440.1 90.783 651 56 2 1 649 1 649 0.0 1202
Msa0644230 AT3G09440.4 90.783 651 56 2 1 649 1 649 0.0 1202
Msa0644230 AT3G09440.2 90.783 651 56 2 1 649 1 649 0.0 1202
Msa0644230 AT3G09440.3 90.783 651 56 2 1 649 1 649 0.0 1202
Msa0644230 AT5G02490.1 89.297 654 64 2 1 649 1 653 0.0 1196
Msa0644230 AT1G16030.1 84.746 649 96 2 1 649 1 646 0.0 1137
Msa0644230 AT1G56410.1 87.399 619 76 1 1 619 1 617 0.0 1122
Msa0644230 AT5G42020.1 64.895 621 212 4 7 625 35 651 0.0 827
Msa0644230 AT5G42020.3 64.895 621 212 4 7 625 40 656 0.0 826
Msa0644230 AT5G28540.1 65.472 614 206 4 7 618 35 644 0.0 825
Msa0644230 AT1G09080.1 62.806 613 223 3 7 618 50 658 0.0 782
Msa0644230 AT1G09080.2 62.806 613 223 3 7 618 40 648 0.0 782
Msa0644230 AT5G02500.2 92.893 394 28 0 1 394 1 394 0.0 771
Msa0644230 AT5G02500.2 76.087 138 27 2 516 649 386 521 5.86e-54 193
Msa0644230 AT5G42020.2 58.615 621 196 9 7 625 35 596 0.0 695
Msa0644230 AT5G09590.1 51.858 592 259 9 10 594 60 632 0.0 560
Msa0644230 AT4G24280.1 49.251 601 281 10 10 605 81 662 0.0 538
Msa0644230 AT5G49910.1 48.315 623 295 11 10 624 81 684 0.0 535
Msa0644230 AT4G37910.1 52.076 578 251 9 10 580 55 613 0.0 531
Msa0644230 AT4G37910.2 52.076 578 251 9 10 580 55 613 0.0 531
Msa0644230 AT2G32120.1 34.180 512 309 9 9 508 30 525 1.58e-87 284
Msa0644230 AT2G32120.2 34.180 512 309 9 9 508 30 525 1.58e-87 284
Msa0644230 AT1G79930.2 35.388 438 267 4 10 434 4 438 3.00e-86 286
Msa0644230 AT1G79930.1 35.388 438 267 4 10 434 4 438 4.02e-86 287
Msa0644230 AT1G79920.1 35.388 438 267 4 10 434 4 438 5.09e-85 282
Msa0644230 AT1G79920.3 35.388 438 267 4 10 434 4 438 7.32e-85 281
Msa0644230 AT1G79920.2 35.388 438 267 4 10 434 4 438 7.32e-85 281
Msa0644230 AT1G79920.4 35.388 438 267 4 10 434 4 438 7.32e-85 281
Msa0644230 AT1G11660.4 27.515 676 401 12 10 619 4 656 6.21e-84 280
Msa0644230 AT1G11660.3 27.515 676 401 12 10 619 4 656 6.21e-84 280
Msa0644230 AT1G11660.1 27.515 676 401 12 10 619 4 656 6.21e-84 280
Msa0644230 AT1G11660.2 27.613 641 375 12 45 619 2 619 1.10e-75 257
Msa0644230 AT4G16660.2 25.487 718 423 16 5 615 46 758 1.87e-62 223
Msa0644230 AT4G16660.1 25.766 718 421 17 5 615 22 734 2.87e-61 219

Find 184 sgRNAs with CRISPR-Local

Find 225 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTAGGAATGGTTGTGTTTC+TGG 0.103620 4_3:-47876397 None:intergenic
AAGGATGCCGGTGTCATTTC+TGG 0.156592 4_3:+47875622 Msa0644230:CDS
CATCTTCTCAATTTCCTCTT+TGG 0.183600 4_3:-47876685 None:intergenic
GAAAATTTGTTGAGAGATTA+TGG 0.185098 4_3:+47874475 Msa0644230:exon
GCAGGAGAACTCTTCAATTT+TGG 0.189005 4_3:+47877389 Msa0644230:three_prime_UTR
TCTTGAATTCTTGAACAAAA+TGG 0.242578 4_3:-47875870 None:intergenic
AGCCTGTGGAGAAGTGTTTA+AGG 0.261872 4_3:+47876097 Msa0644230:CDS
TTGCTGGAAATTTAATAATC+AGG 0.300996 4_3:+47877352 Msa0644230:three_prime_UTR
CCAACCTGGTGTGCTGATTC+AGG 0.309133 4_3:+47876455 Msa0644230:CDS
TCAGATGCTAAGCGATTAAT+TGG 0.310966 4_3:+47875346 Msa0644230:intron
TACTCCTCTCTCCCTTGGTT+TGG 0.315513 4_3:+47876341 Msa0644230:CDS
ATTTGTTTGAAGTTGGTAAT+TGG 0.317398 4_3:+47877413 Msa0644230:three_prime_UTR
TTAATCAACTTCCTCAATCT+TGG 0.333860 4_3:-47877063 None:intergenic
TGAAAACACCTGCTCCTTCT+TGG 0.336551 4_3:-47876418 None:intergenic
TACTGCTGGCGATACACATT+TGG 0.338169 4_3:+47875816 Msa0644230:CDS
ACCTGCGATTGGAATCGATT+TGG 0.340588 4_3:+47874514 Msa0644230:CDS
TGGCCGTTCAAGGTCATTCC+TGG 0.343735 4_3:+47875412 Msa0644230:CDS
GCTGGCAGTGGAAGCGGTGC+TGG 0.344996 4_3:+47877038 Msa0644230:CDS
CCTGCGATTGGAATCGATTT+GGG 0.346174 4_3:+47874515 Msa0644230:CDS
CCTGATGAGGCTGTTGCTTA+TGG 0.347444 4_3:+47876246 Msa0644230:CDS
TGCAAAAGTTGTTGAACCTT+GGG 0.350273 4_3:-47876183 None:intergenic
ATTAAGCTAAACTTTGTTCA+TGG 0.354002 4_3:+47877081 Msa0644230:exon
TGATACATCCTAGCAATGAT+AGG 0.362378 4_3:-47876966 None:intergenic
AGCTCAATATGGATTTGTTT+AGG 0.363242 4_3:+47876064 Msa0644230:CDS
AACTTTGTTCATGGGATTGA+TGG 0.364747 4_3:+47877090 Msa0644230:three_prime_UTR
GTACATGATGTTGTTCTTGT+TGG 0.372027 4_3:+47876144 Msa0644230:CDS
GTAGGAATGGTTGTGTTTCT+GGG 0.381013 4_3:-47876396 None:intergenic
TTATAGTTCACCACAATCAT+AGG 0.383446 4_3:-47875448 None:intergenic
CCATAAGCAACAGCCTCATC+AGG 0.383744 4_3:-47876246 None:intergenic
CATATTCATGTGTTGGTGTT+TGG 0.389658 4_3:+47874543 Msa0644230:CDS
GGATTTGTTTAGGAAGTGTA+TGG 0.401845 4_3:+47876074 Msa0644230:CDS
TTACCGAAAACAGTGTTAGT+AGG 0.404952 4_3:-47874689 None:intergenic
CCTGCTGCTGGCAGTGGAAG+CGG 0.405341 4_3:+47877032 Msa0644230:CDS
GATGTTACTCCTCTCTCCCT+TGG 0.408263 4_3:+47876336 Msa0644230:CDS
CTCAATCTTCTAAGAGCCCT+TGG 0.408771 4_3:-47875922 None:intergenic
TGTGTTTATCAGTACGTAAT+GGG 0.409539 4_3:+47877323 Msa0644230:three_prime_UTR
CAAGGTGCTGGTGGTGATGC+TGG 0.412390 4_3:+47876987 Msa0644230:CDS
CATGAAATTGTGGCCGTTCA+AGG 0.412524 4_3:+47875402 Msa0644230:CDS
CATGATGTTGTTCTTGTTGG+TGG 0.420045 4_3:+47876147 Msa0644230:CDS
GAGATTGCAGAGGCTTATCT+TGG 0.422742 4_3:+47875532 Msa0644230:CDS
ACGTAATGGGTACTGTTTGC+TGG 0.423572 4_3:+47877336 Msa0644230:three_prime_UTR
TGAAATTGATTCATTGTTTG+AGG 0.423673 4_3:+47875999 Msa0644230:CDS
GGAAAGCCTACCCTTGTCAT+TGG 0.428458 4_3:-47876664 None:intergenic
GACTCGTGATAACAATTTGT+TGG 0.428667 4_3:+47876500 Msa0644230:CDS
CAAAGAGGAAATTGAGAAGA+TGG 0.433532 4_3:+47876686 Msa0644230:CDS
GGGTATCCCTCCTGCTCCAA+GGG 0.435715 4_3:+47876539 Msa0644230:CDS
CTGTGTTTATCAGTACGTAA+TGG 0.436173 4_3:+47877322 Msa0644230:three_prime_UTR
TAGGAGAATCAGCGATGTTT+CGG 0.437308 4_3:+47875369 Msa0644230:CDS
TTGCAAAAGTTGTTGAACCT+TGG 0.441982 4_3:-47876184 None:intergenic
TAATCAACTTCCTCAATCTT+GGG 0.446332 4_3:-47877062 None:intergenic
ACTGCTGGCGATACACATTT+GGG 0.449452 4_3:+47875817 Msa0644230:CDS
TTGGGCAAATTTGAGTTGTC+GGG 0.450094 4_3:+47876519 Msa0644230:CDS
TGGAGAGGCTGATGAATTTG+AGG 0.450186 4_3:+47876911 Msa0644230:CDS
TCCCTTGGTTTGGAGACTGC+AGG 0.450627 4_3:+47876351 Msa0644230:CDS
ATTTGTTGAGAGATTATGGC+TGG 0.452082 4_3:+47874479 Msa0644230:exon
GACAAGAAGGCTACAAGTAC+TGG 0.452597 4_3:+47875703 Msa0644230:CDS
CTGCTCCTTCTTGGTAGGAA+TGG 0.452909 4_3:-47876409 None:intergenic
TGAGATCATAGCAAACGATC+AGG 0.454634 4_3:+47874580 Msa0644230:CDS
GTTGGGCAAATTTGAGTTGT+CGG 0.456584 4_3:+47876518 Msa0644230:CDS
CGGGTATCCCTCCTGCTCCA+AGG 0.460140 4_3:+47876538 Msa0644230:CDS
GATGGTCCTGCTGCTGGCAG+TGG 0.462691 4_3:+47877026 Msa0644230:CDS
ACTGCCGCTGCTATTGCCTA+TGG 0.464087 4_3:+47875676 Msa0644230:CDS
CATGGTTCTTATGAAGATGA+AGG 0.464118 4_3:+47875510 Msa0644230:CDS
GATGAAGATGGTCCTGCTGC+TGG 0.465181 4_3:+47877020 Msa0644230:CDS
ACTCGTGATAACAATTTGTT+GGG 0.474189 4_3:+47876501 Msa0644230:CDS
GTGTTTAAGGGATGCTAAGA+TGG 0.480423 4_3:+47876110 Msa0644230:CDS
GAAAATTGAAGATGCTATTG+AGG 0.480599 4_3:+47876857 Msa0644230:CDS
CAATGAATCAATTTCAATGG+TGG 0.482628 4_3:-47875992 None:intergenic
TTAAGCTAAACTTTGTTCAT+GGG 0.484479 4_3:+47877082 Msa0644230:exon
ATAGGCAATAGCAGCGGCAG+TGG 0.488775 4_3:-47875674 None:intergenic
CCGCTTCCACTGCCAGCAGC+AGG 0.490175 4_3:-47877032 None:intergenic
TATTGAGGAAGGTATCTTTG+AGG 0.490881 4_3:+47875786 Msa0644230:CDS
ACTGATACGGAACGTTTGAT+CGG 0.491310 4_3:+47874635 Msa0644230:CDS
CTTGGTTTGGAGACTGCAGG+AGG 0.492769 4_3:+47876354 Msa0644230:CDS
GCAAGATTTCTTCAATGGAA+AGG 0.494283 4_3:+47876203 Msa0644230:CDS
TGTTTCACTTCTTACTATTG+AGG 0.495641 4_3:+47875771 Msa0644230:CDS
AAACAATGAATCAATTTCAA+TGG 0.497295 4_3:-47875995 None:intergenic
ATTCAAGAGGAAGAATAAGA+AGG 0.502948 4_3:+47875885 Msa0644230:CDS
TCAGTAAAAGCAACATAAGA+AGG 0.505406 4_3:-47874617 None:intergenic
GAAATTGATTCATTGTTTGA+GGG 0.510562 4_3:+47876000 Msa0644230:CDS
TGCCTATGGTCTTGACAAGA+AGG 0.512155 4_3:+47875690 Msa0644230:CDS
TGCTGAAGAGATATCTTCCA+TGG 0.512232 4_3:+47875492 Msa0644230:CDS
TGAGGAACACCCCTTGGAGC+AGG 0.513433 4_3:-47876549 None:intergenic
CTGCTTGTGAGAGAGCTAAG+AGG 0.514038 4_3:+47875947 Msa0644230:CDS
TACCGAAAACAGTGTTAGTA+GGG 0.517002 4_3:-47874688 None:intergenic
AAGAATAAGAAGGATATTAG+CGG 0.517633 4_3:+47875895 Msa0644230:CDS
ACGTTGTGAATCGTTGAAGT+AGG 0.520100 4_3:-47875590 None:intergenic
ATAGGTTTGTCAGCAGGGCC+AGG 0.524557 4_3:-47875430 None:intergenic
GGAAATTGAGAAGATGGTGC+AGG 0.525352 4_3:+47876692 Msa0644230:CDS
ATGCTAAGCGATTAATTGGT+AGG 0.528922 4_3:+47875350 Msa0644230:CDS
AAAATTGAAGATGCTATTGA+GGG 0.529908 4_3:+47876858 Msa0644230:CDS
CGGTGCTGGTCCCAAGATTG+AGG 0.530030 4_3:+47877052 Msa0644230:CDS
TGAGGACAAGATGAAGGAGC+TGG 0.532976 4_3:+47876929 Msa0644230:CDS
CACCGCTCAAAATAGCAGCT+TGG 0.534910 4_3:-47876278 None:intergenic
GGGCCAGGAATGACCTTGAA+CGG 0.539439 4_3:-47875415 None:intergenic
AAAGGTGAAGGACCTGCGAT+TGG 0.542152 4_3:+47874503 Msa0644230:CDS
CAATCATAGGTTTGTCAGCA+GGG 0.542843 4_3:-47875435 None:intergenic
ACTTTGTTCATGGGATTGAT+GGG 0.542929 4_3:+47877091 Msa0644230:three_prime_UTR
AGGATATTAGCGGAAATCCA+AGG 0.545644 4_3:+47875905 Msa0644230:CDS
TGTGAATCGTTGAAGTAGGC+AGG 0.547550 4_3:-47875586 None:intergenic
ACAATCATAGGTTTGTCAGC+AGG 0.547735 4_3:-47875436 None:intergenic
TGGTTTGGAGACTGCAGGAG+GGG 0.547823 4_3:+47876356 Msa0644230:CDS
CCCAAATCGATTCCAATCGC+AGG 0.548277 4_3:-47874515 None:intergenic
TTGGTTTGGAGACTGCAGGA+GGG 0.550498 4_3:+47876355 Msa0644230:CDS
GCCTGTGGAGAAGTGTTTAA+GGG 0.550982 4_3:+47876098 Msa0644230:CDS
AATGGTGGTTTGAGCAGTAG+AGG 0.553384 4_3:-47875977 None:intergenic
TGAGAGATTATGGCTGGAAA+AGG 0.553396 4_3:+47874485 Msa0644230:exon
TCAGCAGAGGACAAGACCAC+AGG 0.553510 4_3:+47876618 Msa0644230:CDS
GGGGCTATTCAATGGTTGGA+TGG 0.553727 4_3:+47876879 Msa0644230:CDS
TTTGCAACCCTATCATTGCT+AGG 0.554597 4_3:+47876958 Msa0644230:CDS
ATCATTGCTAGGATGTATCA+AGG 0.555326 4_3:+47876969 Msa0644230:CDS
TTCAAAACGAGCACGAGTAA+TGG 0.556929 4_3:-47876040 None:intergenic
TGGTGATGCTGGAGGAGCCA+TGG 0.557888 4_3:+47876998 Msa0644230:CDS
GTGATCTGAGGAACACCCCT+TGG 0.562871 4_3:-47876555 None:intergenic
TCCCTTAAACACTTCTCCAC+AGG 0.563569 4_3:-47876099 None:intergenic
CAGGCAAGAAATTTGCAAGC+AGG 0.566745 4_3:+47877371 Msa0644230:three_prime_UTR
GTCTCCAAACCAAGGGAGAG+AGG 0.567259 4_3:-47876345 None:intergenic
GGAACAACATATTCATGTGT+TGG 0.568612 4_3:+47874536 Msa0644230:CDS
GGAACACCCCTTGGAGCAGG+AGG 0.568941 4_3:-47876546 None:intergenic
GATACATCCTAGCAATGATA+GGG 0.569465 4_3:-47876965 None:intergenic
TAGGCAATAGCAGCGGCAGT+GGG 0.574624 4_3:-47875673 None:intergenic
AGCAGGACCATCTTCATCCA+TGG 0.574951 4_3:-47877015 None:intergenic
TGAATTTGAGGACAAGATGA+AGG 0.575031 4_3:+47876923 Msa0644230:CDS
GCTAGGATGTATCAAGGTGC+TGG 0.576167 4_3:+47876975 Msa0644230:CDS
TTTGAGGTGAAAGCTACTGC+TGG 0.577257 4_3:+47875802 Msa0644230:CDS
AAAGTACAAGTCTGAGGACG+AGG 0.578393 4_3:+47876722 Msa0644230:CDS
CTTCATCTTCATAAGAACCA+TGG 0.578877 4_3:-47875509 None:intergenic
CACAACCATTCCTACCAAGA+AGG 0.580287 4_3:+47876404 Msa0644230:CDS
CATTCCTACCAAGAAGGAGC+AGG 0.582400 4_3:+47876410 Msa0644230:CDS
TGAGGGTAATGAGAAAGTAC+AGG 0.583385 4_3:+47876299 Msa0644230:CDS
TCACTTCTTACTATTGAGGA+AGG 0.584344 4_3:+47875775 Msa0644230:CDS
TTCTTGTTGGTGGATCGACT+AGG 0.585926 4_3:+47876157 Msa0644230:CDS
TTTCTGGACTCAATGTGTTG+AGG 0.587988 4_3:+47875638 Msa0644230:CDS
CAATCACCAATGACAAGGGT+AGG 0.589395 4_3:+47876658 Msa0644230:CDS
TGCTTTGACATTGATGCCAA+CGG 0.589749 4_3:+47876582 Msa0644230:CDS
TAGTAGGGTTCATAGCAACT+TGG 0.589835 4_3:-47874673 None:intergenic
TCCTGCAGTCTCCAAACCAA+GGG 0.590338 4_3:-47876352 None:intergenic
CTCCTGCAGTCTCCAAACCA+AGG 0.595424 4_3:-47876353 None:intergenic
ATGGGTGGTTCATGATTGCA+AGG 0.596040 4_3:+47877109 Msa0644230:three_prime_UTR
AAAACTATGCATACAACATG+AGG 0.597334 4_3:+47876781 Msa0644230:CDS
CAGCAGAGGACAAGACCACA+GGG 0.598328 4_3:+47876619 Msa0644230:CDS
TTGTTCATGGGATTGATGGG+TGG 0.598913 4_3:+47877094 Msa0644230:three_prime_UTR
AGCCTTCTTGTCAAGACCAT+AGG 0.599068 4_3:-47875692 None:intergenic
TGGTTGGATGGAAATCAACT+TGG 0.605882 4_3:+47876891 Msa0644230:CDS
TAGACCTGAATCAGCACACC+AGG 0.606163 4_3:-47876459 None:intergenic
CAAGGGTAGGCTTTCCAAAG+AGG 0.607535 4_3:+47876671 Msa0644230:CDS
GGATATTAGCGGAAATCCAA+GGG 0.611329 4_3:+47875906 Msa0644230:CDS
TGAGACATTCAGAATACCGT+TGG 0.615112 4_3:-47876598 None:intergenic
ACCAGGTTGGTTATCAGAGT+AGG 0.621601 4_3:-47876442 None:intergenic
GACAAGATGAAGGAGCTGGA+GGG 0.622357 4_3:+47876933 Msa0644230:CDS
TACAGAGTGACATGAAATTG+TGG 0.624043 4_3:+47875392 Msa0644230:CDS
CGTCAAGCTACAAAGGATGC+CGG 0.624953 4_3:+47875610 Msa0644230:CDS
TTCACAACGTCAAGCTACAA+AGG 0.626174 4_3:+47875603 Msa0644230:CDS
TAAGAGCATCAACCCTGATG+AGG 0.628505 4_3:+47876233 Msa0644230:CDS
GAGATCATAGCAAACGATCA+GGG 0.629271 4_3:+47874581 Msa0644230:CDS
ATTATGGCTGGAAAAGGTGA+AGG 0.630083 4_3:+47874491 Msa0644230:exon
GGATGGAAATCAACTTGGAG+AGG 0.632319 4_3:+47876896 Msa0644230:CDS
TGCTGACAAACCTATGATTG+TGG 0.632591 4_3:+47875438 Msa0644230:CDS
GGAGGAGCCATGGATGAAGA+TGG 0.633207 4_3:+47877008 Msa0644230:CDS
CATAAGCAACAGCCTCATCA+GGG 0.633756 4_3:-47876245 None:intergenic
AACACCTGCTCCTTCTTGGT+AGG 0.634088 4_3:-47876414 None:intergenic
CCTGAATCAGCACACCAGGT+TGG 0.637157 4_3:-47876455 None:intergenic
GGACAAGATGAAGGAGCTGG+AGG 0.638733 4_3:+47876932 Msa0644230:CDS
ACCTACTCTGATAACCAACC+TGG 0.640169 4_3:+47876441 Msa0644230:CDS
TCAAAGCAAACAGTGATCTG+AGG 0.640283 4_3:-47876567 None:intergenic
GAAGATGAAGGAGATTGCAG+AGG 0.642514 4_3:+47875522 Msa0644230:CDS
GATTACAATCACCAATGACA+AGG 0.645567 4_3:+47876653 Msa0644230:CDS
GGTGCTGGTGGTGATGCTGG+AGG 0.646421 4_3:+47876990 Msa0644230:CDS
AGGATGTATCAAGGTGCTGG+TGG 0.649243 4_3:+47876978 Msa0644230:CDS
AATTGAAGATGCTATTGAGG+GGG 0.652473 4_3:+47876860 Msa0644230:CDS
TGCTGAAAAGTACAAGTCTG+AGG 0.657032 4_3:+47876716 Msa0644230:CDS
TGGATCGACTAGGATTCCCA+AGG 0.664968 4_3:+47876167 Msa0644230:CDS
TCTACGAAGGAGAAAGAACA+AGG 0.674145 4_3:+47876478 Msa0644230:CDS
GTGCTGATTCAGGTCTACGA+AGG 0.676079 4_3:+47876465 Msa0644230:CDS
TATTCTGAATGTCTCAGCAG+AGG 0.676206 4_3:+47876605 Msa0644230:CDS
TAGGAAGTGTATGGAGCCTG+TGG 0.676933 4_3:+47876083 Msa0644230:CDS
GGTATCCCTCCTGCTCCAAG+GGG 0.680067 4_3:+47876540 Msa0644230:CDS
ATTACAATCACCAATGACAA+GGG 0.682522 4_3:+47876654 Msa0644230:CDS
GAACACCCCTTGGAGCAGGA+GGG 0.684051 4_3:-47876545 None:intergenic
AAATTGAAGATGCTATTGAG+GGG 0.691800 4_3:+47876859 Msa0644230:CDS
GCTGGCGATACACATTTGGG+AGG 0.694962 4_3:+47875820 Msa0644230:CDS
TGTGGTGAACTATAAAGCTG+AGG 0.717089 4_3:+47875456 Msa0644230:CDS
ATTGAGTCCAGAAATGACAC+CGG 0.717355 4_3:-47875629 None:intergenic
AAGACCATAGGCAATAGCAG+CGG 0.731023 4_3:-47875680 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTAAAAAAAACAGATTAA+TGG - chr4_3:47874922-47874941 None:intergenic 10.0%
!! TTGTACATATACATAATTAT+AGG + chr4_3:47875136-47875155 Msa0644230:intron 15.0%
!! CTGAAAAAGAATAAAAACTT+CGG - chr4_3:47874749-47874768 None:intergenic 20.0%
!!! ATAAGGTAGAAAAGTATAAA+AGG + chr4_3:47874971-47874990 Msa0644230:intron 20.0%
!!! AAAAGTATAAAAGGTAGAAA+AGG + chr4_3:47874980-47874999 Msa0644230:intron 20.0%
!! AAAGTATAAAAGGTAGAAAA+GGG + chr4_3:47874981-47875000 Msa0644230:intron 20.0%
!! AAACAATGAATCAATTTCAA+TGG - chr4_3:47875998-47876017 None:intergenic 20.0%
!!! TGTTTTGATTTAGATAATGT+AGG + chr4_3:47877164-47877183 Msa0644230:three_prime_UTR 20.0%
!!! GTTTTGATTTAGATAATGTA+GGG + chr4_3:47877165-47877184 Msa0644230:three_prime_UTR 20.0%
! TCGAATGAATCAAAAAACTA+GGG - chr4_3:47874448-47874467 None:intergenic 25.0%
! ATCGAATGAATCAAAAAACT+AGG - chr4_3:47874449-47874468 None:intergenic 25.0%
!!! GAAAATTTGTTGAGAGATTA+TGG + chr4_3:47874475-47874494 Msa0644230:exon 25.0%
!!! TTATGTTGCTTTTACTGATA+CGG + chr4_3:47874622-47874641 Msa0644230:CDS 25.0%
!!! TGAAGATTTTGATAACAGAA+TGG + chr4_3:47875843-47875862 Msa0644230:CDS 25.0%
! TCTTGAATTCTTGAACAAAA+TGG - chr4_3:47875873-47875892 None:intergenic 25.0%
!! ATTTTGTTCAAGAATTCAAG+AGG + chr4_3:47875872-47875891 Msa0644230:CDS 25.0%
! AAGAATAAGAAGGATATTAG+CGG + chr4_3:47875895-47875914 Msa0644230:CDS 25.0%
!! TGAAATTGATTCATTGTTTG+AGG + chr4_3:47875999-47876018 Msa0644230:CDS 25.0%
!! GAAATTGATTCATTGTTTGA+GGG + chr4_3:47876000-47876019 Msa0644230:CDS 25.0%
! AAAATTGAAGATGCTATTGA+GGG + chr4_3:47876858-47876877 Msa0644230:CDS 25.0%
! ATTAAGCTAAACTTTGTTCA+TGG + chr4_3:47877081-47877100 Msa0644230:exon 25.0%
! TTAAGCTAAACTTTGTTCAT+GGG + chr4_3:47877082-47877101 Msa0644230:exon 25.0%
!!! TTCGCTTTTAATCTTAATGA+TGG + chr4_3:47877214-47877233 Msa0644230:three_prime_UTR 25.0%
! TTGCTGGAAATTTAATAATC+AGG + chr4_3:47877352-47877371 Msa0644230:three_prime_UTR 25.0%
!!! TTTTGGTATTTGTTTGAAGT+TGG + chr4_3:47877406-47877425 Msa0644230:three_prime_UTR 25.0%
!! TTTTTGATTCATTCGATCGA+AGG + chr4_3:47874452-47874471 Msa0644230:five_prime_UTR 30.0%
!! TTTTGATTCATTCGATCGAA+GGG + chr4_3:47874453-47874472 Msa0644230:five_prime_UTR 30.0%
TCAGTAAAAGCAACATAAGA+AGG - chr4_3:47874620-47874639 None:intergenic 30.0%
ATAAAAACTTCGGAAGACTA+AGG - chr4_3:47874739-47874758 None:intergenic 30.0%
! CTGAGAGTAGTTTGTATATA+AGG + chr4_3:47874954-47874973 Msa0644230:intron 30.0%
! TGCTGATCATAATTTTGCAT+TGG + chr4_3:47875190-47875209 Msa0644230:intron 30.0%
TCAGAGCATAATAGTAGAAA+AGG + chr4_3:47875249-47875268 Msa0644230:intron 30.0%
CAGAGCATAATAGTAGAAAA+GGG + chr4_3:47875250-47875269 Msa0644230:intron 30.0%
GTTTCAGTATTTGTCAAGAA+TGG + chr4_3:47875282-47875301 Msa0644230:intron 30.0%
TTATAGTTCACCACAATCAT+AGG - chr4_3:47875451-47875470 None:intergenic 30.0%
!!! AACGTTCTTATTTTCGATCT+TGG + chr4_3:47875733-47875752 Msa0644230:CDS 30.0%
TGTTTCACTTCTTACTATTG+AGG + chr4_3:47875771-47875790 Msa0644230:CDS 30.0%
ATTCAAGAGGAAGAATAAGA+AGG + chr4_3:47875885-47875904 Msa0644230:CDS 30.0%
CAATGAATCAATTTCAATGG+TGG - chr4_3:47875995-47876014 None:intergenic 30.0%
! AGCTCAATATGGATTTGTTT+AGG + chr4_3:47876064-47876083 Msa0644230:CDS 30.0%
! CTTTTGCAAGATTTCTTCAA+TGG + chr4_3:47876198-47876217 Msa0644230:CDS 30.0%
! ACTCGTGATAACAATTTGTT+GGG + chr4_3:47876501-47876520 Msa0644230:CDS 30.0%
ATTACAATCACCAATGACAA+GGG + chr4_3:47876654-47876673 Msa0644230:CDS 30.0%
!!! ATAGTTTTCAAGTGCATTCT+TGG - chr4_3:47876769-47876788 None:intergenic 30.0%
AAAACTATGCATACAACATG+AGG + chr4_3:47876781-47876800 Msa0644230:CDS 30.0%
GAAAATTGAAGATGCTATTG+AGG + chr4_3:47876857-47876876 Msa0644230:CDS 30.0%
AAATTGAAGATGCTATTGAG+GGG + chr4_3:47876859-47876878 Msa0644230:CDS 30.0%
TAATCAACTTCCTCAATCTT+GGG - chr4_3:47877065-47877084 None:intergenic 30.0%
TTAATCAACTTCCTCAATCT+TGG - chr4_3:47877066-47877085 None:intergenic 30.0%
TGTGTTTATCAGTACGTAAT+GGG + chr4_3:47877323-47877342 Msa0644230:three_prime_UTR 30.0%
! ATTTGTTGAGAGATTATGGC+TGG + chr4_3:47874479-47874498 Msa0644230:exon 35.0%
GGAACAACATATTCATGTGT+TGG + chr4_3:47874536-47874555 Msa0644230:CDS 35.0%
! CATATTCATGTGTTGGTGTT+TGG + chr4_3:47874543-47874562 Msa0644230:CDS 35.0%
! AACCCTACTAACACTGTTTT+CGG + chr4_3:47874686-47874705 Msa0644230:CDS 35.0%
TACCGAAAACAGTGTTAGTA+GGG - chr4_3:47874691-47874710 None:intergenic 35.0%
TTACCGAAAACAGTGTTAGT+AGG - chr4_3:47874692-47874711 None:intergenic 35.0%
TCGGAAGACTAAGGATTAAT+AGG - chr4_3:47874730-47874749 None:intergenic 35.0%
CTGATTGTGTTTGCTTGAAT+TGG + chr4_3:47875318-47875337 Msa0644230:intron 35.0%
TCAGATGCTAAGCGATTAAT+TGG + chr4_3:47875346-47875365 Msa0644230:intron 35.0%
ATGCTAAGCGATTAATTGGT+AGG + chr4_3:47875350-47875369 Msa0644230:CDS 35.0%
TACAGAGTGACATGAAATTG+TGG + chr4_3:47875392-47875411 Msa0644230:CDS 35.0%
CTTCATCTTCATAAGAACCA+TGG - chr4_3:47875512-47875531 None:intergenic 35.0%
CATGGTTCTTATGAAGATGA+AGG + chr4_3:47875510-47875529 Msa0644230:CDS 35.0%
!!! GTTCTTATTTTCGATCTTGG+TGG + chr4_3:47875736-47875755 Msa0644230:CDS 35.0%
TCACTTCTTACTATTGAGGA+AGG + chr4_3:47875775-47875794 Msa0644230:CDS 35.0%
!! TATTGAGGAAGGTATCTTTG+AGG + chr4_3:47875786-47875805 Msa0644230:CDS 35.0%
!!! TCGTTTTGAAGAGCTCAATA+TGG + chr4_3:47876053-47876072 Msa0644230:CDS 35.0%
! GGATTTGTTTAGGAAGTGTA+TGG + chr4_3:47876074-47876093 Msa0644230:CDS 35.0%
GTACATGATGTTGTTCTTGT+TGG + chr4_3:47876144-47876163 Msa0644230:CDS 35.0%
TGCAAAAGTTGTTGAACCTT+GGG - chr4_3:47876186-47876205 None:intergenic 35.0%
TTGCAAAAGTTGTTGAACCT+TGG - chr4_3:47876187-47876206 None:intergenic 35.0%
GCAAGATTTCTTCAATGGAA+AGG + chr4_3:47876203-47876222 Msa0644230:CDS 35.0%
GACTCGTGATAACAATTTGT+TGG + chr4_3:47876500-47876519 Msa0644230:CDS 35.0%
GATTACAATCACCAATGACA+AGG + chr4_3:47876653-47876672 Msa0644230:CDS 35.0%
CATCTTCTCAATTTCCTCTT+TGG - chr4_3:47876688-47876707 None:intergenic 35.0%
CAAAGAGGAAATTGAGAAGA+TGG + chr4_3:47876686-47876705 Msa0644230:CDS 35.0%
AATTGAAGATGCTATTGAGG+GGG + chr4_3:47876860-47876879 Msa0644230:CDS 35.0%
TGAATTTGAGGACAAGATGA+AGG + chr4_3:47876923-47876942 Msa0644230:CDS 35.0%
GATACATCCTAGCAATGATA+GGG - chr4_3:47876968-47876987 None:intergenic 35.0%
TGATACATCCTAGCAATGAT+AGG - chr4_3:47876969-47876988 None:intergenic 35.0%
ATCATTGCTAGGATGTATCA+AGG + chr4_3:47876969-47876988 Msa0644230:CDS 35.0%
AACTTTGTTCATGGGATTGA+TGG + chr4_3:47877090-47877109 Msa0644230:three_prime_UTR 35.0%
ACTTTGTTCATGGGATTGAT+GGG + chr4_3:47877091-47877110 Msa0644230:three_prime_UTR 35.0%
CTGTGTTTATCAGTACGTAA+TGG + chr4_3:47877322-47877341 Msa0644230:three_prime_UTR 35.0%
TGAGAGATTATGGCTGGAAA+AGG + chr4_3:47874485-47874504 Msa0644230:exon 40.0%
ATTATGGCTGGAAAAGGTGA+AGG + chr4_3:47874491-47874510 Msa0644230:exon 40.0%
TGAGATCATAGCAAACGATC+AGG + chr4_3:47874580-47874599 Msa0644230:CDS 40.0%
GAGATCATAGCAAACGATCA+GGG + chr4_3:47874581-47874600 Msa0644230:CDS 40.0%
ACTGATACGGAACGTTTGAT+CGG + chr4_3:47874635-47874654 Msa0644230:CDS 40.0%
TAGTAGGGTTCATAGCAACT+TGG - chr4_3:47874676-47874695 None:intergenic 40.0%
CAAGCAAACACAATCAGCTA+AGG - chr4_3:47875315-47875334 None:intergenic 40.0%
TAGGAGAATCAGCGATGTTT+CGG + chr4_3:47875369-47875388 Msa0644230:CDS 40.0%
CAATCATAGGTTTGTCAGCA+GGG - chr4_3:47875438-47875457 None:intergenic 40.0%
ACAATCATAGGTTTGTCAGC+AGG - chr4_3:47875439-47875458 None:intergenic 40.0%
TGCTGACAAACCTATGATTG+TGG + chr4_3:47875438-47875457 Msa0644230:CDS 40.0%
TGTGGTGAACTATAAAGCTG+AGG + chr4_3:47875456-47875475 Msa0644230:CDS 40.0%
TGCTGAAGAGATATCTTCCA+TGG + chr4_3:47875492-47875511 Msa0644230:CDS 40.0%
ACGTTGTGAATCGTTGAAGT+AGG - chr4_3:47875593-47875612 None:intergenic 40.0%
TTCACAACGTCAAGCTACAA+AGG + chr4_3:47875603-47875622 Msa0644230:CDS 40.0%
ATTGAGTCCAGAAATGACAC+CGG - chr4_3:47875632-47875651 None:intergenic 40.0%
TTTCTGGACTCAATGTGTTG+AGG + chr4_3:47875638-47875657 Msa0644230:CDS 40.0%
!! CTTATTTTCGATCTTGGTGG+TGG + chr4_3:47875739-47875758 Msa0644230:CDS 40.0%
AGGATATTAGCGGAAATCCA+AGG + chr4_3:47875905-47875924 Msa0644230:CDS 40.0%
GGATATTAGCGGAAATCCAA+GGG + chr4_3:47875906-47875925 Msa0644230:CDS 40.0%
TTCAAAACGAGCACGAGTAA+TGG - chr4_3:47876043-47876062 None:intergenic 40.0%
GTGTTTAAGGGATGCTAAGA+TGG + chr4_3:47876110-47876129 Msa0644230:CDS 40.0%
CATGATGTTGTTCTTGTTGG+TGG + chr4_3:47876147-47876166 Msa0644230:CDS 40.0%
! TGAGGGTAATGAGAAAGTAC+AGG + chr4_3:47876299-47876318 Msa0644230:CDS 40.0%
GTAGGAATGGTTGTGTTTCT+GGG - chr4_3:47876399-47876418 None:intergenic 40.0%
TCTACGAAGGAGAAAGAACA+AGG + chr4_3:47876478-47876497 Msa0644230:CDS 40.0%
!! GTTGGGCAAATTTGAGTTGT+CGG + chr4_3:47876518-47876537 Msa0644230:CDS 40.0%
!! TTGGGCAAATTTGAGTTGTC+GGG + chr4_3:47876519-47876538 Msa0644230:CDS 40.0%
TCAAAGCAAACAGTGATCTG+AGG - chr4_3:47876570-47876589 None:intergenic 40.0%
TGCTTTGACATTGATGCCAA+CGG + chr4_3:47876582-47876601 Msa0644230:CDS 40.0%
TGAGACATTCAGAATACCGT+TGG - chr4_3:47876601-47876620 None:intergenic 40.0%
TATTCTGAATGTCTCAGCAG+AGG + chr4_3:47876605-47876624 Msa0644230:CDS 40.0%
!!! AATCTTGTTTTTCTGCCCTG+TGG - chr4_3:47876637-47876656 None:intergenic 40.0%
! TGCTGAAAAGTACAAGTCTG+AGG + chr4_3:47876716-47876735 Msa0644230:CDS 40.0%
TGGTTGGATGGAAATCAACT+TGG + chr4_3:47876891-47876910 Msa0644230:CDS 40.0%
TTTGCAACCCTATCATTGCT+AGG + chr4_3:47876958-47876977 Msa0644230:CDS 40.0%
! GCAGGAGAACTCTTCAATTT+TGG + chr4_3:47877389-47877408 Msa0644230:three_prime_UTR 40.0%
ACCTGCGATTGGAATCGATT+TGG + chr4_3:47874514-47874533 Msa0644230:CDS 45.0%
! CCTGCGATTGGAATCGATTT+GGG + chr4_3:47874515-47874534 Msa0644230:CDS 45.0%
CATGAAATTGTGGCCGTTCA+AGG + chr4_3:47875402-47875421 Msa0644230:CDS 45.0%
GAAGATGAAGGAGATTGCAG+AGG + chr4_3:47875522-47875541 Msa0644230:CDS 45.0%
GAGATTGCAGAGGCTTATCT+TGG + chr4_3:47875532-47875551 Msa0644230:CDS 45.0%
TGTGAATCGTTGAAGTAGGC+AGG - chr4_3:47875589-47875608 None:intergenic 45.0%
AAGACCATAGGCAATAGCAG+CGG - chr4_3:47875683-47875702 None:intergenic 45.0%
! TGCCTATGGTCTTGACAAGA+AGG + chr4_3:47875690-47875709 Msa0644230:CDS 45.0%
AGCCTTCTTGTCAAGACCAT+AGG - chr4_3:47875695-47875714 None:intergenic 45.0%
GACAAGAAGGCTACAAGTAC+TGG + chr4_3:47875703-47875722 Msa0644230:CDS 45.0%
! TTTGAGGTGAAAGCTACTGC+TGG + chr4_3:47875802-47875821 Msa0644230:CDS 45.0%
TACTGCTGGCGATACACATT+TGG + chr4_3:47875816-47875835 Msa0644230:CDS 45.0%
ACTGCTGGCGATACACATTT+GGG + chr4_3:47875817-47875836 Msa0644230:CDS 45.0%
! CTCAATCTTCTAAGAGCCCT+TGG - chr4_3:47875925-47875944 None:intergenic 45.0%
AATGGTGGTTTGAGCAGTAG+AGG - chr4_3:47875980-47875999 None:intergenic 45.0%
!! AGCCTGTGGAGAAGTGTTTA+AGG + chr4_3:47876097-47876116 Msa0644230:CDS 45.0%
!! GCCTGTGGAGAAGTGTTTAA+GGG + chr4_3:47876098-47876117 Msa0644230:CDS 45.0%
TCCCTTAAACACTTCTCCAC+AGG - chr4_3:47876102-47876121 None:intergenic 45.0%
! TTCTTGTTGGTGGATCGACT+AGG + chr4_3:47876157-47876176 Msa0644230:CDS 45.0%
!! TAAGAGCATCAACCCTGATG+AGG + chr4_3:47876233-47876252 Msa0644230:CDS 45.0%
CATAAGCAACAGCCTCATCA+GGG - chr4_3:47876248-47876267 None:intergenic 45.0%
!!! GTCCAAGCTGCTATTTTGAG+CGG + chr4_3:47876276-47876295 Msa0644230:CDS 45.0%
GGTAGGAATGGTTGTGTTTC+TGG - chr4_3:47876400-47876419 None:intergenic 45.0%
CACAACCATTCCTACCAAGA+AGG + chr4_3:47876404-47876423 Msa0644230:CDS 45.0%
TGAAAACACCTGCTCCTTCT+TGG - chr4_3:47876421-47876440 None:intergenic 45.0%
ACCTACTCTGATAACCAACC+TGG + chr4_3:47876441-47876460 Msa0644230:CDS 45.0%
!! ACCAGGTTGGTTATCAGAGT+AGG - chr4_3:47876445-47876464 None:intergenic 45.0%
CAATCACCAATGACAAGGGT+AGG + chr4_3:47876658-47876677 Msa0644230:CDS 45.0%
GGAAATTGAGAAGATGGTGC+AGG + chr4_3:47876692-47876711 Msa0644230:CDS 45.0%
AAAGTACAAGTCTGAGGACG+AGG + chr4_3:47876722-47876741 Msa0644230:CDS 45.0%
CTATTGAGGGGGCTATTCAA+TGG + chr4_3:47876871-47876890 Msa0644230:CDS 45.0%
GGATGGAAATCAACTTGGAG+AGG + chr4_3:47876896-47876915 Msa0644230:CDS 45.0%
TGGAGAGGCTGATGAATTTG+AGG + chr4_3:47876911-47876930 Msa0644230:CDS 45.0%
TTGTTCATGGGATTGATGGG+TGG + chr4_3:47877094-47877113 Msa0644230:three_prime_UTR 45.0%
ATGGGTGGTTCATGATTGCA+AGG + chr4_3:47877109-47877128 Msa0644230:three_prime_UTR 45.0%
!! ACGTAATGGGTACTGTTTGC+TGG + chr4_3:47877336-47877355 Msa0644230:three_prime_UTR 45.0%
CAGGCAAGAAATTTGCAAGC+AGG + chr4_3:47877371-47877390 Msa0644230:three_prime_UTR 45.0%
AAAGGTGAAGGACCTGCGAT+TGG + chr4_3:47874503-47874522 Msa0644230:CDS 50.0%
! CCCAAATCGATTCCAATCGC+AGG - chr4_3:47874518-47874537 None:intergenic 50.0%
CGTCAAGCTACAAAGGATGC+CGG + chr4_3:47875610-47875629 Msa0644230:CDS 50.0%
!! AAGGATGCCGGTGTCATTTC+TGG + chr4_3:47875622-47875641 Msa0644230:CDS 50.0%
CTGCTTGTGAGAGAGCTAAG+AGG + chr4_3:47875947-47875966 Msa0644230:CDS 50.0%
! TAGGAAGTGTATGGAGCCTG+TGG + chr4_3:47876083-47876102 Msa0644230:CDS 50.0%
TGGATCGACTAGGATTCCCA+AGG + chr4_3:47876167-47876186 Msa0644230:CDS 50.0%
CCATAAGCAACAGCCTCATC+AGG - chr4_3:47876249-47876268 None:intergenic 50.0%
! CCTGATGAGGCTGTTGCTTA+TGG + chr4_3:47876246-47876265 Msa0644230:CDS 50.0%
CACCGCTCAAAATAGCAGCT+TGG - chr4_3:47876281-47876300 None:intergenic 50.0%
!!! AGCTGCTATTTTGAGCGGTG+AGG + chr4_3:47876281-47876300 Msa0644230:CDS 50.0%
!!! GCTGCTATTTTGAGCGGTGA+GGG + chr4_3:47876282-47876301 Msa0644230:CDS 50.0%
GATGTTACTCCTCTCTCCCT+TGG + chr4_3:47876336-47876355 Msa0644230:CDS 50.0%
TACTCCTCTCTCCCTTGGTT+TGG + chr4_3:47876341-47876360 Msa0644230:CDS 50.0%
TCCTGCAGTCTCCAAACCAA+GGG - chr4_3:47876355-47876374 None:intergenic 50.0%
!! TTGGTTTGGAGACTGCAGGA+GGG + chr4_3:47876355-47876374 Msa0644230:CDS 50.0%
CTGCTCCTTCTTGGTAGGAA+TGG - chr4_3:47876412-47876431 None:intergenic 50.0%
CATTCCTACCAAGAAGGAGC+AGG + chr4_3:47876410-47876429 Msa0644230:CDS 50.0%
AACACCTGCTCCTTCTTGGT+AGG - chr4_3:47876417-47876436 None:intergenic 50.0%
TAGACCTGAATCAGCACACC+AGG - chr4_3:47876462-47876481 None:intergenic 50.0%
!! GTGCTGATTCAGGTCTACGA+AGG + chr4_3:47876465-47876484 Msa0644230:CDS 50.0%
GGAAAGCCTACCCTTGTCAT+TGG - chr4_3:47876667-47876686 None:intergenic 50.0%
CAAGGGTAGGCTTTCCAAAG+AGG + chr4_3:47876671-47876690 Msa0644230:CDS 50.0%
! GGACGAGGAGCACAAAAAGA+AGG + chr4_3:47876737-47876756 Msa0644230:CDS 50.0%
! CGAGGAGCACAAAAAGAAGG+TGG + chr4_3:47876740-47876759 Msa0644230:CDS 50.0%
GGAGCACAAAAAGAAGGTGG+AGG + chr4_3:47876743-47876762 Msa0644230:CDS 50.0%
TGAGGGGGCTATTCAATGGT+TGG + chr4_3:47876875-47876894 Msa0644230:CDS 50.0%
GGGGCTATTCAATGGTTGGA+TGG + chr4_3:47876879-47876898 Msa0644230:CDS 50.0%
TGAGGACAAGATGAAGGAGC+TGG + chr4_3:47876929-47876948 Msa0644230:CDS 50.0%
GACAAGATGAAGGAGCTGGA+GGG + chr4_3:47876933-47876952 Msa0644230:CDS 50.0%
GCTAGGATGTATCAAGGTGC+TGG + chr4_3:47876975-47876994 Msa0644230:CDS 50.0%
!! AGGATGTATCAAGGTGCTGG+TGG + chr4_3:47876978-47876997 Msa0644230:CDS 50.0%
AGCAGGACCATCTTCATCCA+TGG - chr4_3:47877018-47877037 None:intergenic 50.0%
TGGCCGTTCAAGGTCATTCC+TGG + chr4_3:47875412-47875431 Msa0644230:CDS 55.0%
GGGCCAGGAATGACCTTGAA+CGG - chr4_3:47875418-47875437 None:intergenic 55.0%
ATAGGTTTGTCAGCAGGGCC+AGG - chr4_3:47875433-47875452 None:intergenic 55.0%
TAGGCAATAGCAGCGGCAGT+GGG - chr4_3:47875676-47875695 None:intergenic 55.0%
ATAGGCAATAGCAGCGGCAG+TGG - chr4_3:47875677-47875696 None:intergenic 55.0%
ACTGCCGCTGCTATTGCCTA+TGG + chr4_3:47875676-47875695 Msa0644230:CDS 55.0%
GCTGGCGATACACATTTGGG+AGG + chr4_3:47875820-47875839 Msa0644230:CDS 55.0%
GTCTCCAAACCAAGGGAGAG+AGG - chr4_3:47876348-47876367 None:intergenic 55.0%
!! TCCCTTGGTTTGGAGACTGC+AGG + chr4_3:47876351-47876370 Msa0644230:CDS 55.0%
CTCCTGCAGTCTCCAAACCA+AGG - chr4_3:47876356-47876375 None:intergenic 55.0%
!! CTTGGTTTGGAGACTGCAGG+AGG + chr4_3:47876354-47876373 Msa0644230:CDS 55.0%
! TGGTTTGGAGACTGCAGGAG+GGG + chr4_3:47876356-47876375 Msa0644230:CDS 55.0%
CCTGAATCAGCACACCAGGT+TGG - chr4_3:47876458-47876477 None:intergenic 55.0%
!! CCAACCTGGTGTGCTGATTC+AGG + chr4_3:47876455-47876474 Msa0644230:CDS 55.0%
GTGATCTGAGGAACACCCCT+TGG - chr4_3:47876558-47876577 None:intergenic 55.0%
TCAGCAGAGGACAAGACCAC+AGG + chr4_3:47876618-47876637 Msa0644230:CDS 55.0%
CAGCAGAGGACAAGACCACA+GGG + chr4_3:47876619-47876638 Msa0644230:CDS 55.0%
GGACAAGATGAAGGAGCTGG+AGG + chr4_3:47876932-47876951 Msa0644230:CDS 55.0%
GGAGGAGCCATGGATGAAGA+TGG + chr4_3:47877008-47877027 Msa0644230:CDS 55.0%
GATGAAGATGGTCCTGCTGC+TGG + chr4_3:47877020-47877039 Msa0644230:CDS 55.0%
GGGTATCCCTCCTGCTCCAA+GGG + chr4_3:47876539-47876558 Msa0644230:CDS 60.0%
GGTATCCCTCCTGCTCCAAG+GGG + chr4_3:47876540-47876559 Msa0644230:CDS 60.0%
GAACACCCCTTGGAGCAGGA+GGG - chr4_3:47876548-47876567 None:intergenic 60.0%
TGAGGAACACCCCTTGGAGC+AGG - chr4_3:47876552-47876571 None:intergenic 60.0%
!! CAAGGTGCTGGTGGTGATGC+TGG + chr4_3:47876987-47877006 Msa0644230:CDS 60.0%
! TGGTGATGCTGGAGGAGCCA+TGG + chr4_3:47876998-47877017 Msa0644230:CDS 60.0%
!! CGGTGCTGGTCCCAAGATTG+AGG + chr4_3:47877052-47877071 Msa0644230:CDS 60.0%
CGGGTATCCCTCCTGCTCCA+AGG + chr4_3:47876538-47876557 Msa0644230:CDS 65.0%
GGAACACCCCTTGGAGCAGG+AGG - chr4_3:47876549-47876568 None:intergenic 65.0%
!! GGTGCTGGTGGTGATGCTGG+AGG + chr4_3:47876990-47877009 Msa0644230:CDS 65.0%
GATGGTCCTGCTGCTGGCAG+TGG + chr4_3:47877026-47877045 Msa0644230:CDS 65.0%
CCTGCTGCTGGCAGTGGAAG+CGG + chr4_3:47877032-47877051 Msa0644230:CDS 65.0%
CCGCTTCCACTGCCAGCAGC+AGG - chr4_3:47877035-47877054 None:intergenic 70.0%
GCTGGCAGTGGAAGCGGTGC+TGG + chr4_3:47877038-47877057 Msa0644230:CDS 70.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 47874368 47877435 47874368 ID=Msa0644230;Name=Msa0644230
chr4_3 mRNA 47874368 47877435 47874368 ID=Msa0644230-mRNA-1;Parent=Msa0644230;Name=Msa0644230-mRNA-1;_AED=0.02;_eAED=0.02;_QI=126|1|1|1|1|1|2|350|649
chr4_3 exon 47874368 47874707 47874368 ID=Msa0644230-mRNA-1:exon:11344;Parent=Msa0644230-mRNA-1
chr4_3 exon 47875350 47877435 47875350 ID=Msa0644230-mRNA-1:exon:11345;Parent=Msa0644230-mRNA-1
chr4_3 five_prime_UTR 47874368 47874493 47874368 ID=Msa0644230-mRNA-1:five_prime_utr;Parent=Msa0644230-mRNA-1
chr4_3 CDS 47874494 47874707 47874494 ID=Msa0644230-mRNA-1:cds;Parent=Msa0644230-mRNA-1
chr4_3 CDS 47875350 47877085 47875350 ID=Msa0644230-mRNA-1:cds;Parent=Msa0644230-mRNA-1
chr4_3 three_prime_UTR 47877086 47877435 47877086 ID=Msa0644230-mRNA-1:three_prime_utr;Parent=Msa0644230-mRNA-1
Gene Sequence

>Msa0644230

ATGGCTGGAAAAGGTGAAGGACCTGCGATTGGAATCGATTTGGGAACAACATATTCATGTGTTGGTGTTTGGCAACATGATCGAGTTGAGATCATAGCAAACGATCAGGGTAACAGAACTACACCTTCTTATGTTGCTTTTACTGATACGGAACGTTTGATCGGTGATGCTGCAAAGAACCAAGTTGCTATGAACCCTACTAACACTGTTTTCGATGCTAAGCGATTAATTGGTAGGAGAATCAGCGATGTTTCGGTACAGAGTGACATGAAATTGTGGCCGTTCAAGGTCATTCCTGGCCCTGCTGACAAACCTATGATTGTGGTGAACTATAAAGCTGAGGAGAAACAATTTTCTGCTGAAGAGATATCTTCCATGGTTCTTATGAAGATGAAGGAGATTGCAGAGGCTTATCTTGGTACAACGATTAAGAATGCTGTCGTAACTGTGCCTGCCTACTTCAACGATTCACAACGTCAAGCTACAAAGGATGCCGGTGTCATTTCTGGACTCAATGTGTTGAGGATCATTAATGAGCCCACTGCCGCTGCTATTGCCTATGGTCTTGACAAGAAGGCTACAAGTACTGGTGAGAAGAACGTTCTTATTTTCGATCTTGGTGGTGGAACTTTTGATGTTTCACTTCTTACTATTGAGGAAGGTATCTTTGAGGTGAAAGCTACTGCTGGCGATACACATTTGGGAGGTGAAGATTTTGATAACAGAATGGTGAACCATTTTGTTCAAGAATTCAAGAGGAAGAATAAGAAGGATATTAGCGGAAATCCAAGGGCTCTTAGAAGATTGAGAACTGCTTGTGAGAGAGCTAAGAGGACTCTTTCCTCTACTGCTCAAACCACCATTGAAATTGATTCATTGTTTGAGGGTATTGACTTTTACACAACCATTACTCGTGCTCGTTTTGAAGAGCTCAATATGGATTTGTTTAGGAAGTGTATGGAGCCTGTGGAGAAGTGTTTAAGGGATGCTAAGATGGATAAGAACAGTGTACATGATGTTGTTCTTGTTGGTGGATCGACTAGGATTCCCAAGGTTCAACAACTTTTGCAAGATTTCTTCAATGGAAAGGAGCTTTGTAAGAGCATCAACCCTGATGAGGCTGTTGCTTATGGCGCTGCAGTCCAAGCTGCTATTTTGAGCGGTGAGGGTAATGAGAAAGTACAGGATCTGTTGTTGCTTGATGTTACTCCTCTCTCCCTTGGTTTGGAGACTGCAGGAGGGGTCATGACTGTGTTAATTCCCAGAAACACAACCATTCCTACCAAGAAGGAGCAGGTGTTTTCAACCTACTCTGATAACCAACCTGGTGTGCTGATTCAGGTCTACGAAGGAGAAAGAACAAGGACTCGTGATAACAATTTGTTGGGCAAATTTGAGTTGTCGGGTATCCCTCCTGCTCCAAGGGGTGTTCCTCAGATCACTGTTTGCTTTGACATTGATGCCAACGGTATTCTGAATGTCTCAGCAGAGGACAAGACCACAGGGCAGAAAAACAAGATTACAATCACCAATGACAAGGGTAGGCTTTCCAAAGAGGAAATTGAGAAGATGGTGCAGGATGCTGAAAAGTACAAGTCTGAGGACGAGGAGCACAAAAAGAAGGTGGAGGCCAAGAATGCACTTGAAAACTATGCATACAACATGAGGAACACAATCAAAGATGACAAGATTGCTTCGAAGTTGTCTGCTGATGATAAGAAGAAAATTGAAGATGCTATTGAGGGGGCTATTCAATGGTTGGATGGAAATCAACTTGGAGAGGCTGATGAATTTGAGGACAAGATGAAGGAGCTGGAGGGCATTTGCAACCCTATCATTGCTAGGATGTATCAAGGTGCTGGTGGTGATGCTGGAGGAGCCATGGATGAAGATGGTCCTGCTGCTGGCAGTGGAAGCGGTGCTGGTCCCAAGATTGAGGAAGTTGATTAA
Protein sequence

>Msa0644230

MAGKGEGPAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRISDVSVQSDMKLWPFKVIPGPADKPMIVVNYKAEEKQFSAEEISSMVLMKMKEIAEAYLGTTIKNAVVTVPAYFNDSQRQATKDAGVISGLNVLRIINEPTAAAIAYGLDKKATSTGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTHLGGEDFDNRMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLFEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKNSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAGGVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFELSGIPPAPRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKMVQDAEKYKSEDEEHKKKVEAKNALENYAYNMRNTIKDDKIASKLSADDKKKIEDAIEGAIQWLDGNQLGEADEFEDKMKELEGICNPIIARMYQGAGGDAGGAMDEDGPAAGSGSGAGPKIEEVD*