AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0548590

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0548590 MtrunA17_Chr4g0031901 83.816 587 22 6 1 516 1 585 0.0 951
Msa0548590 MtrunA17_Chr7g0238351 61.644 584 137 10 14 516 4 581 0.0 694
Msa0548590 MtrunA17_Chr7g0237441 59.831 590 147 8 7 516 1 580 0.0 684
Msa0548590 MtrunA17_Chr7g0237491 60.374 588 143 9 11 516 1 580 0.0 675
Msa0548590 MtrunA17_Chr7g0238941 66.802 491 114 5 72 516 91 578 0.0 672
Msa0548590 MtrunA17_Chr7g0238981 55.835 557 166 7 37 516 40 593 0.0 617
Msa0548590 MtrunA17_Chr4g0005921 48.770 488 196 6 72 516 81 557 2.02e-160 467
Msa0548590 MtrunA17_Chr5g0429511 48.057 489 196 8 72 516 84 558 8.65e-159 463
Msa0548590 MtrunA17_Chr3g0104061 47.951 488 197 6 72 516 83 556 1.64e-158 462
Msa0548590 MtrunA17_Chr5g0404341 47.648 489 199 7 72 516 91 566 2.29e-157 459
Msa0548590 MtrunA17_Chr5g0437681 47.782 496 199 10 72 516 81 567 4.68e-155 454
Msa0548590 MtrunA17_Chr5g0404361 42.535 576 242 11 14 516 52 611 9.50e-152 447
Msa0548590 MtrunA17_Chr5g0436461 46.516 488 204 6 72 516 81 554 3.37e-151 443
Msa0548590 MtrunA17_Chr3g0114211 47.695 499 189 11 72 516 90 570 2.45e-150 442
Msa0548590 MtrunA17_Chr5g0431581 45.344 494 206 11 72 516 82 560 6.37e-133 397
Msa0548590 MtrunA17_Chr8g0346581 43.522 494 217 10 72 516 91 571 6.55e-129 387
Msa0548590 MtrunA17_Chr7g0240991 40.845 497 235 9 72 516 82 571 3.64e-123 372
Msa0548590 MtrunA17_Chr4g0008521 41.400 500 228 8 72 516 83 572 3.61e-122 370
Msa0548590 MtrunA17_Chr2g0278811 37.765 519 243 7 72 516 95 607 3.13e-120 366
Msa0548590 MtrunA17_Chr7g0240971 42.400 500 222 12 72 516 82 570 1.04e-114 350
Msa0548590 MtrunA17_Chr3g0133861 34.808 339 164 10 216 506 1 330 1.26e-48 171
Msa0548590 MtrunA17_Chr3g0133871 52.308 130 62 0 72 201 89 218 1.34e-42 151
Msa0548590 MtrunA17_Chr5g0436431 40.854 164 88 3 313 475 32 187 9.66e-35 130
Msa0548590 MtrunA17_Chr4g0056511 27.529 425 248 13 73 476 85 470 2.01e-31 128
Msa0548590 MtrunA17_Chr3g0118321 27.567 526 253 21 72 516 84 562 3.26e-30 124
Msa0548590 MtrunA17_Chr3g0118331 27.626 514 253 23 72 507 83 555 5.75e-30 123
Msa0548590 MtrunA17_Chr3g0079321 26.353 425 253 14 73 476 87 472 1.44e-26 113
Msa0548590 MtrunA17_Chr8g0382691 25.941 505 270 19 72 507 92 561 1.75e-24 107
Msa0548590 MtrunA17_Chr7g0234061 25.986 431 247 18 72 476 86 470 2.00e-22 100
Msa0548590 MtrunA17_Chr1g0157341 24.694 409 263 14 72 476 86 453 1.33e-18 89.0
Msa0548590 MtrunA17_Chr3g0121161 29.646 226 148 7 72 296 123 338 2.34e-17 85.1
Msa0548590 MtrunA17_Chr3g0121151 29.646 226 148 7 72 296 123 338 2.91e-17 84.7
Msa0548590 MtrunA17_Chr3g0143671 23.077 429 245 14 72 476 87 454 3.02e-17 84.7
Msa0548590 MtrunA17_Chr3g0121171 29.646 226 148 7 72 296 1233 1448 3.86e-17 85.5
Msa0548590 MtrunA17_Chr3g0121171 29.646 226 148 7 72 296 658 873 4.29e-17 85.1
Msa0548590 MtrunA17_Chr3g0121171 29.646 226 148 7 72 296 83 298 1.33e-16 83.6
Msa0548590 MtrunA17_Chr3g0121171 29.204 226 149 7 72 296 1799 2014 9.31e-16 80.9
Msa0548590 MtrunA17_Chr4g0024861 28.910 211 138 5 72 281 84 283 7.76e-17 83.6
Msa0548590 MtrunA17_Chr4g0043261 29.386 228 146 8 73 299 83 296 1.38e-16 82.8
Msa0548590 MtrunA17_Chr4g0043251 30.144 209 137 7 73 279 85 286 1.45e-16 82.4
Msa0548590 MtrunA17_Chr5g0402831 28.708 209 135 5 72 279 88 283 2.37e-15 78.6
Msa0548590 MtrunA17_Chr4g0038671 27.962 211 139 6 72 281 87 285 1.06e-14 76.6
Msa0548590 MtrunA17_Chr2g0322441 28.910 211 138 5 72 281 87 286 3.23e-14 75.1
Msa0548590 MtrunA17_Chr8g0374261 23.325 403 267 14 73 474 87 448 1.62e-12 69.7
Msa0548590 MtrunA17_Chr8g0374271 25.664 226 150 6 72 296 88 296 1.82e-12 69.7
Msa0548590 MtrunA17_Chr3g0118351 25.937 347 168 13 226 507 5 327 3.11e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0548590 AT2G29130.1 59.375 576 149 9 15 516 9 573 0.0 669
Msa0548590 AT5G60020.1 65.261 498 118 6 72 516 82 577 0.0 662
Msa0548590 AT5G60020.3 65.261 498 118 6 72 516 50 545 0.0 660
Msa0548590 AT5G60020.2 71.014 414 107 5 72 474 82 493 0.0 609
Msa0548590 AT2G38080.1 48.255 487 198 5 72 516 84 558 3.21e-159 464
Msa0548590 AT5G05390.1 48.450 516 202 11 51 516 64 565 1.28e-155 456
Msa0548590 AT2G40370.1 47.421 504 197 10 72 516 86 580 3.25e-153 450
Msa0548590 AT5G01190.1 46.830 489 204 7 72 516 82 558 1.25e-152 447
Msa0548590 AT5G01190.2 46.830 489 204 7 72 516 68 544 1.27e-152 447
Msa0548590 AT5G03260.1 47.551 490 197 9 72 516 83 557 6.04e-152 446
Msa0548590 AT5G58910.2 45.214 491 213 7 72 516 87 567 2.89e-148 437
Msa0548590 AT5G58910.3 45.214 491 213 7 72 516 86 566 4.23e-148 436
Msa0548590 AT5G58910.1 44.628 484 212 7 79 516 50 523 5.90e-143 422
Msa0548590 AT2G30210.1 45.859 495 209 9 72 516 85 570 7.77e-143 423
Msa0548590 AT1G18140.1 44.821 502 214 11 72 516 86 581 1.92e-142 422
Msa0548590 AT5G07130.1 45.582 498 209 12 72 516 81 569 1.47e-132 397
Msa0548590 AT2G46570.2 40.927 496 227 10 72 516 4 484 3.39e-130 387
Msa0548590 AT2G46570.1 40.927 496 227 10 72 516 89 569 2.07e-129 389
Msa0548590 AT5G07130.2 45.233 493 208 12 77 516 1 484 3.15e-129 385
Msa0548590 AT5G07130.3 45.233 493 208 12 77 516 1 484 3.15e-129 385
Msa0548590 AT5G09360.1 40.162 493 231 12 72 516 93 569 1.33e-119 363
Msa0548590 AT3G09220.1 42.259 478 216 8 72 499 83 550 1.81e-119 363
Msa0548590 AT3G09220.2 42.208 462 207 8 88 499 2 453 2.30e-114 347
Msa0548590 AT5G48100.1 40.123 486 229 10 72 506 79 553 4.06e-113 347
Msa0548590 AT5G01050.1 39.479 499 233 11 72 516 85 568 2.63e-112 345
Msa0548590 AT5G01040.1 39.235 497 235 11 72 516 85 566 2.59e-110 340
Msa0548590 AT5G21105.1 27.843 510 261 18 72 506 96 573 3.09e-35 139
Msa0548590 AT5G21100.1 25.536 513 265 17 72 507 82 554 4.54e-35 139
Msa0548590 AT5G21105.3 30.575 435 236 19 72 474 96 496 4.75e-34 135
Msa0548590 AT4G25240.2 26.869 428 246 15 73 476 87 471 9.90e-29 120
Msa0548590 AT4G25240.1 26.869 428 246 15 73 476 87 471 9.90e-29 120
Msa0548590 AT4G39830.1 27.169 438 251 17 72 474 94 498 1.49e-27 117
Msa0548590 AT5G51480.1 26.698 427 249 16 73 476 86 471 7.23e-27 114
Msa0548590 AT4G12420.1 25.822 426 256 13 72 476 82 468 3.71e-25 109
Msa0548590 AT4G12420.2 25.822 426 256 13 72 476 82 468 3.71e-25 109
Msa0548590 AT4G39830.2 26.376 436 249 16 76 474 3 403 1.13e-24 107
Msa0548590 AT4G39830.3 26.450 431 245 16 81 474 6 401 3.33e-24 106
Msa0548590 AT1G75790.1 27.518 407 253 15 72 474 84 452 3.50e-24 106
Msa0548590 AT5G48450.2 26.764 411 261 16 72 476 86 462 4.53e-22 100
Msa0548590 AT5G48450.1 26.764 411 261 16 72 476 86 462 7.10e-22 99.8
Msa0548590 AT4G39830.4 25.287 435 242 15 76 474 3 390 3.77e-20 93.6
Msa0548590 AT5G21105.2 26.741 359 185 11 199 506 51 382 4.07e-19 89.7
Msa0548590 AT1G55560.1 29.787 235 154 7 73 306 84 308 2.06e-18 89.0
Msa0548590 AT1G21860.1 28.319 226 145 6 72 296 86 295 4.29e-18 87.8
Msa0548590 AT1G21850.1 28.000 225 145 6 73 296 87 295 5.50e-18 87.4
Msa0548590 AT3G13390.1 28.444 225 150 6 72 295 85 299 2.47e-16 82.4
Msa0548590 AT1G55570.1 29.018 224 148 7 73 295 87 300 2.48e-16 82.4
Msa0548590 AT4G37160.2 27.273 209 140 4 72 279 88 285 9.46e-16 80.1
Msa0548590 AT4G37160.1 27.273 209 140 4 72 279 88 285 2.33e-15 79.3
Msa0548590 AT3G13400.2 27.660 235 159 7 73 306 85 309 3.36e-15 78.6
Msa0548590 AT3G13400.1 27.660 235 159 7 73 306 85 309 3.36e-15 78.6
Msa0548590 AT1G76160.1 27.876 226 146 7 72 296 85 294 5.54e-15 78.2
Msa0548590 AT5G66920.1 23.171 410 260 13 72 474 94 455 1.17e-14 77.0
Msa0548590 AT1G41830.1 27.434 226 147 7 72 296 86 295 6.74e-14 74.7
Msa0548590 AT2G23630.1 24.146 410 267 14 72 478 87 455 1.15e-12 70.9
Msa0548590 AT2G23630.2 24.146 410 267 14 72 478 92 460 1.16e-12 70.9
Msa0548590 AT4G22010.1 26.794 209 141 6 72 279 83 280 1.49e-12 70.5

Find 148 sgRNAs with CRISPR-Local

Find 246 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCATTTCCATGTAAATTTA+AGG 0.077214 4_1:-42776481 Msa0548590:three_prime_UTR
GGCACATTTGCAGGGTATTT+TGG 0.173773 4_1:+42777656 None:intergenic
TCTGATGCCTACACCATTAA+TGG 0.176037 4_1:-42778702 Msa0548590:CDS
ATTGCAGGATACAAGCATTT+TGG 0.187461 4_1:-42777318 Msa0548590:CDS
TTTGAATTTGAACTATTATT+TGG 0.212461 4_1:+42777554 None:intergenic
CTTCTCCAACAACATTTCTT+TGG 0.228697 4_1:-42777482 Msa0548590:CDS
CATGTAAATTTAAGGATTTA+TGG 0.246154 4_1:-42776473 Msa0548590:three_prime_UTR
CATTTCTTTGGAACTAATAA+TGG 0.256030 4_1:-42777470 Msa0548590:CDS
AACCTCCCCTGGATTTGTTT+TGG 0.259173 4_1:-42779880 Msa0548590:CDS
AATATCATTGTACTTACTTA+AGG 0.293923 4_1:-42776294 Msa0548590:three_prime_UTR
GTTTCTTTAGTAAGGAAAAT+TGG 0.294923 4_1:+42776538 None:intergenic
TAACTCTGACCTGTTTGAAT+TGG 0.297459 4_1:+42779154 None:intergenic
CTTTGATGTGCACCTTAGTT+GGG 0.307238 4_1:-42776668 Msa0548590:CDS
AATGTGGCATTTGGGTAATT+TGG 0.312994 4_1:+42777872 None:intergenic
TACACCATTAATGGGTTTCC+TGG 0.319870 4_1:-42778693 Msa0548590:CDS
TTGGTGTAGTAATCAACATT+TGG 0.322522 4_1:+42776570 None:intergenic
TGGTGTAGTAATCAACATTT+GGG 0.326399 4_1:+42776571 None:intergenic
AGTCATCCTTTGCATCTTCA+TGG 0.334535 4_1:-42777287 Msa0548590:CDS
AAGGAAGTCCCCATTCTCTT+TGG 0.338186 4_1:-42778992 Msa0548590:intron
ACCTGAACTCTAGTGTTATA+TGG 0.345887 4_1:+42777344 None:intergenic
GCTTGTGCAATTACAGCTTC+AGG 0.346802 4_1:+42778753 None:intergenic
TCCTTAAATTTACATGGAAA+TGG 0.350319 4_1:+42776480 None:intergenic
TGGTTAAGAGCTACTGTCTA+TGG 0.350969 4_1:-42779070 Msa0548590:CDS
ATATCATTGTACTTACTTAA+GGG 0.353683 4_1:-42776293 Msa0548590:three_prime_UTR
GGATTTAATTTCTTTGTTGT+TGG 0.356145 4_1:-42777266 Msa0548590:CDS
TTACTAACCAAAGTATTGTT+TGG 0.366291 4_1:+42777389 None:intergenic
CATGGAAATGGAAATGGAAA+TGG 0.370370 4_1:+42776492 None:intergenic
CTGATGCCTACACCATTAAT+GGG 0.371595 4_1:-42778701 Msa0548590:CDS
AATTTACATGGAAATGGAAA+TGG 0.372179 4_1:+42776486 None:intergenic
TATTTGGTCCTTGACAAGTT+TGG 0.374143 4_1:+42777570 None:intergenic
AGAAAAGGGTTTCTTTAGTA+AGG 0.376132 4_1:+42776530 None:intergenic
AGGTCCAGGAAACCCATTAA+TGG 0.377126 4_1:+42778689 None:intergenic
AACTCTGACCTGTTTGAATT+GGG 0.379719 4_1:+42779155 None:intergenic
AACAAAATTAGTATCATTGA+TGG 0.379750 4_1:+42777715 None:intergenic
CAACATTTGGGGAGATCCTT+AGG 0.380323 4_1:+42776583 None:intergenic
AGCATGTAGAATGTGGCATT+TGG 0.380488 4_1:+42777863 None:intergenic
GACAAAGAGGAACTCTCTTC+TGG 0.381182 4_1:-42779108 Msa0548590:CDS
AAAAGCATGGTTACCGTGAA+TGG 0.383433 4_1:-42779629 Msa0548590:CDS
GTACCCTGCCCTGTGAAATA+TGG 0.385825 4_1:+42777833 None:intergenic
CATAAATCCTTAAATTTACA+TGG 0.392188 4_1:+42776474 None:intergenic
TAGAGTTCAGGTTATATTGC+AGG 0.393539 4_1:-42777333 Msa0548590:CDS
TACCGTGAATGGGAAGTTCC+CGG 0.400570 4_1:-42779618 Msa0548590:CDS
AACTATCTTGTACTCATTCA+AGG 0.405635 4_1:-42779988 Msa0548590:five_prime_UTR
GAGAATGGGGACTTCCTTGT+AGG 0.406530 4_1:+42778997 None:intergenic
GTGTAGGCATCAGAAACATT+TGG 0.413299 4_1:+42778711 None:intergenic
GCATGTAGAATGTGGCATTT+GGG 0.416680 4_1:+42777864 None:intergenic
ACTTTGATGTGCACCTTAGT+TGG 0.416806 4_1:-42776669 Msa0548590:CDS
ACCTTCCTCTTAACCTCCCC+TGG 0.418136 4_1:-42779891 Msa0548590:CDS
AAAATCAAAAGGTGTAGAAA+AGG 0.418687 4_1:+42776515 None:intergenic
GTTGGGGATTGAGGATGGCT+TGG 0.419155 4_1:-42776651 Msa0548590:CDS
ACCATATAACACTAGAGTTC+AGG 0.422189 4_1:-42777345 Msa0548590:CDS
GTATAGTTAAACGGCCTTAA+CGG 0.423945 4_1:+42777422 None:intergenic
TTCGACAATTCCACAGTAGC+TGG 0.425573 4_1:-42777809 Msa0548590:CDS
AATTTCTTTGTTGTTGGTCA+AGG 0.431250 4_1:-42777260 Msa0548590:intron
TTAAGGCCGTTTAACTATAC+AGG 0.432215 4_1:-42777419 Msa0548590:CDS
GATAAGATTCATTGTGCCTT+GGG 0.433565 4_1:+42779032 None:intergenic
CAAATCCAGGGGAGGTTAAG+AGG 0.435761 4_1:+42779886 None:intergenic
GTGTACAACTTCACCATTGT+TGG 0.440870 4_1:-42779130 Msa0548590:CDS
TTCATATGTACCAAAGAGAA+TGG 0.444243 4_1:+42778982 None:intergenic
AACGGCCTTAACGGTACAAC+CGG 0.445112 4_1:+42777431 None:intergenic
TGTAGGCATCAGAAACATTT+GGG 0.450843 4_1:+42778712 None:intergenic
AATGAGTACAAGATAGTTGA+AGG 0.454547 4_1:+42779993 None:intergenic
AACTCCTCCAAACAATACTT+TGG 0.459111 4_1:-42777396 Msa0548590:CDS
TGCACCTTAGTTGGGGATTG+AGG 0.460367 4_1:-42776660 Msa0548590:CDS
GGATAAGATTCATTGTGCCT+TGG 0.462220 4_1:+42779031 None:intergenic
GGAAAATTGGCAGAGTCAAT+TGG 0.466366 4_1:+42776551 None:intergenic
CCTTAGTTGGGGATTGAGGA+TGG 0.469162 4_1:-42776656 Msa0548590:CDS
TCATTGATGGCTGGAAGCAC+AGG 0.471762 4_1:+42777728 None:intergenic
GGAGTTCCTGTATAGTTAAA+CGG 0.473322 4_1:+42777413 None:intergenic
TCATATGTACCAAAGAGAAT+GGG 0.475158 4_1:+42778983 None:intergenic
TCTGGCATGCTCATTTCTCA+TGG 0.475847 4_1:-42779090 Msa0548590:CDS
AACAATACTTTGGTTAGTAA+TGG 0.478654 4_1:-42777386 Msa0548590:CDS
AAATCAAAAGGTGTAGAAAA+GGG 0.479134 4_1:+42776516 None:intergenic
TAGTTCCAAAGAAATGTTGT+TGG 0.480695 4_1:+42777477 None:intergenic
ATTGCACAAGCTCTGCAGAC+AGG 0.483287 4_1:-42778741 Msa0548590:CDS
ACTCAATGCCCAATTCAAAC+AGG 0.483829 4_1:-42779163 Msa0548590:CDS
AATGGAAATGGAAAATCAAA+AGG 0.487974 4_1:+42776504 None:intergenic
GAAGTAGCCAAAACAAATCC+AGG 0.492773 4_1:+42779873 None:intergenic
TTCACCATTGTTGGACAAAG+AGG 0.495747 4_1:-42779121 Msa0548590:CDS
GGGCATTGAGTTATGTATGA+TGG 0.498399 4_1:+42779175 None:intergenic
TGATACAATGAAAGAAAGAC+TGG 0.498850 4_1:+42776386 None:intergenic
TGGTCCAAGTATGATGGTAT+TGG 0.499764 4_1:+42777928 None:intergenic
TGCAGAGAGTGAAGTGTGTC+CGG 0.503416 4_1:-42776427 Msa0548590:three_prime_UTR
GTATGGTTGAAACAGGTGTT+TGG 0.506981 4_1:-42776705 Msa0548590:intron
ACCCGGGAACTTCCCATTCA+CGG 0.507216 4_1:+42779616 None:intergenic
GAGCAATTGTAAAGAGGTCC+AGG 0.507787 4_1:+42778675 None:intergenic
TGTTGGAGAAGTGCTATAGA+AGG 0.510179 4_1:+42777494 None:intergenic
CCTCTTTACAATTGCTCCAA+AGG 0.511152 4_1:-42778669 Msa0548590:intron
AAAGCATGGTTACCGTGAAT+GGG 0.513288 4_1:-42779628 Msa0548590:CDS
CTTCGAAGTGGTTGGTCGGA+TGG 0.513745 4_1:-42779199 Msa0548590:CDS
AAAATTGCCTCCTCCTCCTA+AGG 0.514093 4_1:-42776599 Msa0548590:CDS
ATGGCTTGGATTGTTGAGGA+TGG 0.516858 4_1:-42776637 Msa0548590:CDS
ATTCATTGTGCCTTGGGAGA+AGG 0.517082 4_1:+42779038 None:intergenic
AGAATGGGGACTTCCTTGTA+GGG 0.517590 4_1:+42778998 None:intergenic
TTTGATGTGCACCTTAGTTG+GGG 0.517799 4_1:-42776667 Msa0548590:CDS
TAGACCATATTTCACAGGGC+AGG 0.518387 4_1:-42777837 Msa0548590:CDS
GACAGATACATACAAATTGA+AGG 0.527305 4_1:-42778080 Msa0548590:intron
CTAGCTAGACCATATTTCAC+AGG 0.529517 4_1:-42777842 Msa0548590:CDS
GATAAAACGCTTGAATCATA+CGG 0.529877 4_1:+42776172 None:intergenic
TACAAATTGAAGGTGAAGCC+AGG 0.530121 4_1:-42778070 Msa0548590:CDS
GAGGATGGCTTGGATTGTTG+AGG 0.530967 4_1:-42776641 Msa0548590:CDS
AGAACATTTGTGGTTTGACC+TGG 0.536892 4_1:+42777908 None:intergenic
CTAACCAAAGTATTGTTTGG+AGG 0.538509 4_1:+42777392 None:intergenic
TTGCACAAGCTCTGCAGACA+GGG 0.538737 4_1:-42778740 Msa0548590:CDS
ACCGTGAATGGGAAGTTCCC+GGG 0.543276 4_1:-42779617 Msa0548590:intron
GAGTCCAATACCATCATACT+TGG 0.543746 4_1:-42777932 Msa0548590:CDS
CCATCCTCAATCCCCAACTA+AGG 0.552441 4_1:+42776656 None:intergenic
CCTTTGGAGCAATTGTAAAG+AGG 0.556173 4_1:+42778669 None:intergenic
TCTGCAGATAAGGTTGGCAA+AGG 0.561585 4_1:-42779672 Msa0548590:intron
TGTCACTGTTCCATTATCAA+AGG 0.562747 4_1:-42779943 Msa0548590:CDS
GGGAGATCCTTAGGAGGAGG+AGG 0.564134 4_1:+42776592 None:intergenic
GAATCATCATATACTAGTGC+CGG 0.571850 4_1:+42776408 None:intergenic
TTAAATCCATGAAGATGCAA+AGG 0.575251 4_1:+42777281 None:intergenic
TTCTAAAATTCCAGCTACTG+TGG 0.577452 4_1:+42777799 None:intergenic
TTGGGGAGATCCTTAGGAGG+AGG 0.578506 4_1:+42776589 None:intergenic
AGGAAACCCATTAATGGTGT+AGG 0.579472 4_1:+42778695 None:intergenic
AAATTAGTATCATTGATGGC+TGG 0.579956 4_1:+42777719 None:intergenic
TTGACCTGGTCCAAGTATGA+TGG 0.586103 4_1:+42777922 None:intergenic
GACCTCTCATCCTTCTCCCA+AGG 0.596454 4_1:-42779048 Msa0548590:CDS
TCCAGGGGAGGTTAAGAGGA+AGG 0.601150 4_1:+42779890 None:intergenic
TGAGACAACTTCGAAGTGGT+TGG 0.602069 4_1:-42779207 Msa0548590:CDS
AAGTAGCCAAAACAAATCCA+GGG 0.606149 4_1:+42779874 None:intergenic
ACAACTTCGAAGTGGTTGGT+CGG 0.608285 4_1:-42779203 Msa0548590:CDS
AGTTCCTCTTTGTCCAACAA+TGG 0.608427 4_1:+42779117 None:intergenic
AGACCATATTTCACAGGGCA+GGG 0.613998 4_1:-42777836 Msa0548590:CDS
CATTTGGGGAGATCCTTAGG+AGG 0.640212 4_1:+42776586 None:intergenic
TGCACAGTATATGCAAAATG+TGG 0.642341 4_1:+42776446 None:intergenic
TGCCTTGGGAGAAGGATGAG+AGG 0.645507 4_1:+42779046 None:intergenic
ATGATGGTATTGGACTCGAA+TGG 0.647830 4_1:+42777938 None:intergenic
CATATGTACCAAAGAGAATG+GGG 0.650276 4_1:+42778984 None:intergenic
TGGACTAGTTCCTAGTCCAA+CGG 0.652330 4_1:+42777604 None:intergenic
GACAAATATGCATAGGTGAG+TGG 0.653174 4_1:-42778788 Msa0548590:intron
AGCCAAAACAAATCCAGGGG+AGG 0.654034 4_1:+42779878 None:intergenic
AAATATGCATAGGTGAGTGG+TGG 0.659012 4_1:-42778785 Msa0548590:intron
TCTAGCTAGCATGTAGAATG+TGG 0.659413 4_1:+42777856 None:intergenic
TGCACAAGCTCTGCAGACAG+GGG 0.660853 4_1:-42778739 Msa0548590:CDS
TAAGGTTGGCAAAGGTTACG+AGG 0.665174 4_1:-42779664 Msa0548590:intron
AAGATGCATCTGCTTCGACA+AGG 0.668532 4_1:+42777969 None:intergenic
GCACAAGCTCTGCAGACAGG+GGG 0.670081 4_1:-42778738 Msa0548590:CDS
AACTAAGGTGCACATCAAAG+TGG 0.677837 4_1:+42776671 None:intergenic
TAGCTAGACCATATTTCACA+GGG 0.683532 4_1:-42777841 Msa0548590:CDS
AATACCATCATACTTGGACC+AGG 0.697209 4_1:-42777926 Msa0548590:CDS
TAGTAATGGTACAAAGACAG+TGG 0.701875 4_1:-42777372 Msa0548590:CDS
AGTAGCCAAAACAAATCCAG+GGG 0.722267 4_1:+42779875 None:intergenic
AGGGGAGGTTAAGAGGAAGG+TGG 0.734018 4_1:+42779893 None:intergenic
GGTGTAGTAATCAACATTTG+GGG 0.734375 4_1:+42776572 None:intergenic
GGTGTGAGACAACTTCGAAG+TGG 0.745320 4_1:-42779211 Msa0548590:CDS
TATCTTGTACTCATTCAAGG+TGG 0.745577 4_1:-42779985 Msa0548590:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATTATAAATTTATACTG+AGG + chr4_1:42777228-42777247 None:intergenic 10.0%
!! ATAAATAATTTATTATACTC+TGG - chr4_1:42777243-42777262 Msa0548590:intron 10.0%
!! ACTATATGATATATAATATT+TGG + chr4_1:42779345-42779364 None:intergenic 10.0%
!!! AACTTTTTATTCAAAAAAAA+AGG - chr4_1:42779914-42779933 Msa0548590:CDS 10.0%
!!! ACTTTTTATTCAAAAAAAAA+GGG - chr4_1:42779915-42779934 Msa0548590:CDS 10.0%
!! TTAAGTGATAAATATTTGAA+AGG + chr4_1:42776397-42776416 None:intergenic 15.0%
!! AAAATAGTATATGATATGAA+AGG - chr4_1:42777341-42777360 Msa0548590:CDS 15.0%
!!! TTTTTATTTGTTTGATCATT+TGG + chr4_1:42778412-42778431 None:intergenic 15.0%
!! TTTGAATTTGAACTATTATT+TGG + chr4_1:42778625-42778644 None:intergenic 15.0%
!!! ATGTATTTTTCTCATATTTT+TGG - chr4_1:42779962-42779981 Msa0548590:CDS 15.0%
!! ACTACATATGTTATACAATT+TGG + chr4_1:42776439-42776458 None:intergenic 20.0%
!!! TTTTATTATTTCTGCAGATA+AGG - chr4_1:42776494-42776513 Msa0548590:three_prime_UTR 20.0%
!! AATAATTCATTTCATGCATA+TGG + chr4_1:42776893-42776912 None:intergenic 20.0%
!! TGTACTAATATTGACAATAA+TGG - chr4_1:42776912-42776931 Msa0548590:intron 20.0%
!!! AAATTAGTTTGTAAAATGAG+AGG + chr4_1:42777979-42777998 None:intergenic 20.0%
!!! TTTTTGAAAGATGAATTGAT+TGG - chr4_1:42778056-42778075 Msa0548590:CDS 20.0%
!!! AGTTTTTAATAGAACATTTG+TGG + chr4_1:42778281-42778300 None:intergenic 20.0%
!! AACAAAATTAGTATCATTGA+TGG + chr4_1:42778464-42778483 None:intergenic 20.0%
!!! AAGTTTTTGTCAATAGTTTT+AGG + chr4_1:42778544-42778563 None:intergenic 20.0%
!!! AACTAATAATGGTTTTACAA+CGG - chr4_1:42778717-42778736 Msa0548590:CDS 20.0%
!! CATAAATCCTTAAATTTACA+TGG + chr4_1:42779705-42779724 None:intergenic 20.0%
!! CATGTAAATTTAAGGATTTA+TGG - chr4_1:42779703-42779722 Msa0548590:intron 20.0%
!! AATATCATTGTACTTACTTA+AGG - chr4_1:42779882-42779901 Msa0548590:CDS 20.0%
!! ATATCATTGTACTTACTTAA+GGG - chr4_1:42779883-42779902 Msa0548590:CDS 20.0%
! CATATGTTATACAATTTGGT+AGG + chr4_1:42776435-42776454 None:intergenic 25.0%
!!! AAACTAAGTGTTGAGAAAAT+AGG - chr4_1:42776740-42776759 Msa0548590:intron 25.0%
! ATATTGTATGTTGTACATCA+TGG - chr4_1:42776852-42776871 Msa0548590:intron 25.0%
!!! TGGTATTTTATATTTCTAGC+TGG - chr4_1:42777263-42777282 Msa0548590:CDS 25.0%
!!! CTGGAGTTTATACTTTTTTA+AGG - chr4_1:42777282-42777301 Msa0548590:CDS 25.0%
! TATCTTTGACAAATATGCAT+AGG - chr4_1:42777381-42777400 Msa0548590:CDS 25.0%
!! AACATTTTCTTTATCCTAAG+TGG + chr4_1:42777931-42777950 None:intergenic 25.0%
!!! ATTGGTTTGTTATCATATCA+TGG - chr4_1:42778074-42778093 Msa0548590:CDS 25.0%
!!! AAAAACTTTTTCTTTACCGT+TGG - chr4_1:42778556-42778575 Msa0548590:intron 25.0%
!!! CATTTCTTTGGAACTAATAA+TGG - chr4_1:42778706-42778725 Msa0548590:CDS 25.0%
!!! TTACTAACCAAAGTATTGTT+TGG + chr4_1:42778790-42778809 None:intergenic 25.0%
! AACAATACTTTGGTTAGTAA+TGG - chr4_1:42778790-42778809 Msa0548590:intron 25.0%
!! GGATTTAATTTCTTTGTTGT+TGG - chr4_1:42778910-42778929 Msa0548590:intron 25.0%
!! ACTATTTTCATAAACTATCC+TGG - chr4_1:42779202-42779221 Msa0548590:CDS 25.0%
!!! ATCTATTTGCTATGAATGTA+TGG - chr4_1:42779454-42779473 Msa0548590:intron 25.0%
! GTTTCTTTAGTAAGGAAAAT+TGG + chr4_1:42779641-42779660 None:intergenic 25.0%
!! AAATCAAAAGGTGTAGAAAA+GGG + chr4_1:42779663-42779682 None:intergenic 25.0%
!! AAAATCAAAAGGTGTAGAAA+AGG + chr4_1:42779664-42779683 None:intergenic 25.0%
! AATGGAAATGGAAAATCAAA+AGG + chr4_1:42779675-42779694 None:intergenic 25.0%
! AATTTACATGGAAATGGAAA+TGG + chr4_1:42779693-42779712 None:intergenic 25.0%
! TCCATTTCCATGTAAATTTA+AGG - chr4_1:42779695-42779714 Msa0548590:intron 25.0%
! TCCTTAAATTTACATGGAAA+TGG + chr4_1:42779699-42779718 None:intergenic 25.0%
!!! TTTTTGGTCACTTGAAAATT+AGG + chr4_1:42779829-42779848 None:intergenic 25.0%
! TTCAAGTGACCAAAAAATAA+AGG - chr4_1:42779833-42779852 Msa0548590:intron 25.0%
AATGAGTACAAGATAGTTGA+AGG + chr4_1:42776186-42776205 None:intergenic 30.0%
AACTATCTTGTACTCATTCA+AGG - chr4_1:42776188-42776207 Msa0548590:three_prime_UTR 30.0%
!! ATTATTTCTGCAGATAAGGT+TGG - chr4_1:42776498-42776517 Msa0548590:three_prime_UTR 30.0%
AAAATAGTTCTTACCAATGC+AGG + chr4_1:42776657-42776676 None:intergenic 30.0%
! TGTGAGTTTTGCAGTTAATT+AGG + chr4_1:42776719-42776738 None:intergenic 30.0%
TGAGAAAATAGGATTTGAAC+TGG - chr4_1:42776751-42776770 Msa0548590:intron 30.0%
TGCATGCATGATAAAAAAAC+AGG - chr4_1:42776939-42776958 Msa0548590:intron 30.0%
TCATATGTACCAAAGAGAAT+GGG + chr4_1:42777196-42777215 None:intergenic 30.0%
TTCATATGTACCAAAGAGAA+TGG + chr4_1:42777197-42777216 None:intergenic 30.0%
GACAGATACATACAAATTGA+AGG - chr4_1:42778096-42778115 Msa0548590:intron 30.0%
! CGTAATAGATATGTTTTTCC+TGG + chr4_1:42778127-42778146 None:intergenic 30.0%
! AAATTAGTATCATTGATGGC+TGG + chr4_1:42778460-42778479 None:intergenic 30.0%
!!! CTTGACAAGTTTGGTTTTTT+GGG + chr4_1:42778600-42778619 None:intergenic 30.0%
TAGTTCCAAAGAAATGTTGT+TGG + chr4_1:42778702-42778721 None:intergenic 30.0%
TTAAATCCATGAAGATGCAA+AGG + chr4_1:42778898-42778917 None:intergenic 30.0%
!! AATTTCTTTGTTGTTGGTCA+AGG - chr4_1:42778916-42778935 Msa0548590:intron 30.0%
CAACAAGATTAAACTTTGCA+GGG + chr4_1:42778969-42778988 None:intergenic 30.0%
TCAACAAGATTAAACTTTGC+AGG + chr4_1:42778970-42778989 None:intergenic 30.0%
ACTTATTTAAGCTGATCTGT+TGG + chr4_1:42779318-42779337 None:intergenic 30.0%
TATGAATGTATGGTTGAAAC+AGG - chr4_1:42779464-42779483 Msa0548590:intron 30.0%
TGGTGTAGTAATCAACATTT+GGG + chr4_1:42779608-42779627 None:intergenic 30.0%
TTGGTGTAGTAATCAACATT+TGG + chr4_1:42779609-42779628 None:intergenic 30.0%
AGAAAAGGGTTTCTTTAGTA+AGG + chr4_1:42779649-42779668 None:intergenic 30.0%
TGATACAATGAAAGAAAGAC+TGG + chr4_1:42779793-42779812 None:intergenic 30.0%
AAGTGACCAAAAAATAAAGG+TGG - chr4_1:42779836-42779855 Msa0548590:CDS 30.0%
AGTGACCAAAAAATAAAGGT+GGG - chr4_1:42779837-42779856 Msa0548590:CDS 30.0%
GATAAAACGCTTGAATCATA+CGG + chr4_1:42780007-42780026 None:intergenic 30.0%
TATCTTGTACTCATTCAAGG+TGG - chr4_1:42776191-42776210 Msa0548590:three_prime_UTR 35.0%
TGTCACTGTTCCATTATCAA+AGG - chr4_1:42776233-42776252 Msa0548590:three_prime_UTR 35.0%
GCAAAAAGAGCCTTTGATAA+TGG + chr4_1:42776246-42776265 None:intergenic 35.0%
AAGTAGCCAAAACAAATCCA+GGG + chr4_1:42776305-42776324 None:intergenic 35.0%
CAACGACATTTGATATGCTT+GGG + chr4_1:42776779-42776798 None:intergenic 35.0%
ACAACGACATTTGATATGCT+TGG + chr4_1:42776780-42776799 None:intergenic 35.0%
TGTACATCATGGATCTAGTT+AGG - chr4_1:42776863-42776882 Msa0548590:intron 35.0%
GCATGATAAAAAAACAGGCA+TGG - chr4_1:42776944-42776963 Msa0548590:intron 35.0%
AACTCTGACCTGTTTGAATT+GGG + chr4_1:42777024-42777043 None:intergenic 35.0%
TAACTCTGACCTGTTTGAAT+TGG + chr4_1:42777025-42777044 None:intergenic 35.0%
GATAAGATTCATTGTGCCTT+GGG + chr4_1:42777147-42777166 None:intergenic 35.0%
! TCCTTTTCAAAAACCCTACA+AGG - chr4_1:42777165-42777184 Msa0548590:intron 35.0%
!! TCCTTGTAGGGTTTTTGAAA+AGG + chr4_1:42777169-42777188 None:intergenic 35.0%
CATATGTACCAAAGAGAATG+GGG + chr4_1:42777195-42777214 None:intergenic 35.0%
TGTAGGCATCAGAAACATTT+GGG + chr4_1:42777467-42777486 None:intergenic 35.0%
GAAAAAACCAAGTTCCACTT+AGG - chr4_1:42777914-42777933 Msa0548590:CDS 35.0%
TCTTTATCCTAAGTGGAACT+TGG + chr4_1:42777924-42777943 None:intergenic 35.0%
AATGTGGCATTTGGGTAATT+TGG + chr4_1:42778307-42778326 None:intergenic 35.0%
TAGCTAGACCATATTTCACA+GGG - chr4_1:42778335-42778354 Msa0548590:intron 35.0%
!! TTCTAAAATTCCAGCTACTG+TGG + chr4_1:42778380-42778399 None:intergenic 35.0%
!!! GAAGCACAGGTTTTAGAATA+TGG + chr4_1:42778438-42778457 None:intergenic 35.0%
!!! TAGTTTTAGGCACATTTGCA+GGG + chr4_1:42778531-42778550 None:intergenic 35.0%
!!! ATAGTTTTAGGCACATTTGC+AGG + chr4_1:42778532-42778551 None:intergenic 35.0%
! TTTTTCTTTACCGTTGGACT+AGG - chr4_1:42778562-42778581 Msa0548590:intron 35.0%
!!! CCTTGACAAGTTTGGTTTTT+TGG + chr4_1:42778601-42778620 None:intergenic 35.0%
CCAAAAAACCAAACTTGTCA+AGG - chr4_1:42778598-42778617 Msa0548590:intron 35.0%
TATTTGGTCCTTGACAAGTT+TGG + chr4_1:42778609-42778628 None:intergenic 35.0%
CTTCTCCAACAACATTTCTT+TGG - chr4_1:42778694-42778713 Msa0548590:CDS 35.0%
!! TGGTTTTACAACGGATTTTC+CGG - chr4_1:42778726-42778745 Msa0548590:CDS 35.0%
GTATAGTTAAACGGCCTTAA+CGG + chr4_1:42778757-42778776 None:intergenic 35.0%
TTAAGGCCGTTTAACTATAC+AGG - chr4_1:42778757-42778776 Msa0548590:CDS 35.0%
GGAGTTCCTGTATAGTTAAA+CGG + chr4_1:42778766-42778785 None:intergenic 35.0%
AACTCCTCCAAACAATACTT+TGG - chr4_1:42778780-42778799 Msa0548590:intron 35.0%
!! CTAACCAAAGTATTGTTTGG+AGG + chr4_1:42778787-42778806 None:intergenic 35.0%
TAGTAATGGTACAAAGACAG+TGG - chr4_1:42778804-42778823 Msa0548590:intron 35.0%
ACCATATAACACTAGAGTTC+AGG - chr4_1:42778831-42778850 Msa0548590:intron 35.0%
ACCTGAACTCTAGTGTTATA+TGG + chr4_1:42778835-42778854 None:intergenic 35.0%
! TAGAGTTCAGGTTATATTGC+AGG - chr4_1:42778843-42778862 Msa0548590:intron 35.0%
! ATTGCAGGATACAAGCATTT+TGG - chr4_1:42778858-42778877 Msa0548590:intron 35.0%
! TTGCAGGATACAAGCATTTT+GGG - chr4_1:42778859-42778878 Msa0548590:intron 35.0%
!!! TTTGTTGTTGGTCAAGGTTT+TGG - chr4_1:42778922-42778941 Msa0548590:intron 35.0%
! TGATTGACTATGGTTCTGTT+TGG - chr4_1:42779065-42779084 Msa0548590:CDS 35.0%
!! CTTATTTTCGTAAGTTCTCC+AGG + chr4_1:42779223-42779242 None:intergenic 35.0%
GGTGTAGTAATCAACATTTG+GGG + chr4_1:42779607-42779626 None:intergenic 35.0%
CATGGAAATGGAAATGGAAA+TGG + chr4_1:42779687-42779706 None:intergenic 35.0%
TGCACAGTATATGCAAAATG+TGG + chr4_1:42779733-42779752 None:intergenic 35.0%
GAATCATCATATACTAGTGC+CGG + chr4_1:42779771-42779790 None:intergenic 35.0%
!!! AACCACCCACCTTTATTTTT+TGG + chr4_1:42779845-42779864 None:intergenic 35.0%
AGTAGCCAAAACAAATCCAG+GGG + chr4_1:42776304-42776323 None:intergenic 40.0%
GAAGTAGCCAAAACAAATCC+AGG + chr4_1:42776306-42776325 None:intergenic 40.0%
!!! GTTTTGGCTACTTCACAACA+TGG - chr4_1:42776312-42776331 Msa0548590:three_prime_UTR 40.0%
! AAAAGCATGGTTACCGTGAA+TGG - chr4_1:42776547-42776566 Msa0548590:three_prime_UTR 40.0%
AAAGCATGGTTACCGTGAAT+GGG - chr4_1:42776548-42776567 Msa0548590:three_prime_UTR 40.0%
CTCGAATTGTTGTACGAGAA+GGG - chr4_1:42776584-42776603 Msa0548590:CDS 40.0%
TCGAATTGTTGTACGAGAAG+GGG - chr4_1:42776585-42776604 Msa0548590:CDS 40.0%
! AGGGGACAGATTATTGGTTA+AGG - chr4_1:42776603-42776622 Msa0548590:CDS 40.0%
CAATGCAGGCTGATATTGTT+TGG + chr4_1:42776643-42776662 None:intergenic 40.0%
CAAACAATATCAGCCTGCAT+TGG - chr4_1:42776641-42776660 Msa0548590:CDS 40.0%
GTGTGTGAGAGTAAAACTCA+TGG + chr4_1:42776811-42776830 None:intergenic 40.0%
!! GGGCATTGAGTTATGTATGA+TGG + chr4_1:42777004-42777023 None:intergenic 40.0%
ACTCAATGCCCAATTCAAAC+AGG - chr4_1:42777013-42777032 Msa0548590:intron 40.0%
GTGTACAACTTCACCATTGT+TGG - chr4_1:42777046-42777065 Msa0548590:intron 40.0%
TTCACCATTGTTGGACAAAG+AGG - chr4_1:42777055-42777074 Msa0548590:intron 40.0%
AGTTCCTCTTTGTCCAACAA+TGG + chr4_1:42777062-42777081 None:intergenic 40.0%
TGGTTAAGAGCTACTGTCTA+TGG - chr4_1:42777106-42777125 Msa0548590:intron 40.0%
GGATAAGATTCATTGTGCCT+TGG + chr4_1:42777148-42777167 None:intergenic 40.0%
GACAAATATGCATAGGTGAG+TGG - chr4_1:42777388-42777407 Msa0548590:CDS 40.0%
AAATATGCATAGGTGAGTGG+TGG - chr4_1:42777391-42777410 Msa0548590:CDS 40.0%
GTGTAGGCATCAGAAACATT+TGG + chr4_1:42777468-42777487 None:intergenic 40.0%
TCTGATGCCTACACCATTAA+TGG - chr4_1:42777474-42777493 Msa0548590:CDS 40.0%
CTGATGCCTACACCATTAAT+GGG - chr4_1:42777475-42777494 Msa0548590:CDS 40.0%
AGGAAACCCATTAATGGTGT+AGG + chr4_1:42777484-42777503 None:intergenic 40.0%
!! TACACCATTAATGGGTTTCC+TGG - chr4_1:42777483-42777502 Msa0548590:CDS 40.0%
!! CCTTTGGAGCAATTGTAAAG+AGG + chr4_1:42777510-42777529 None:intergenic 40.0%
CCTCTTTACAATTGCTCCAA+AGG - chr4_1:42777507-42777526 Msa0548590:CDS 40.0%
ACGTGCAGCTTGTATATAAC+TGG - chr4_1:42777533-42777552 Msa0548590:CDS 40.0%
TACAAATTGAAGGTGAAGCC+AGG - chr4_1:42778106-42778125 Msa0548590:intron 40.0%
! ATGATGGTATTGGACTCGAA+TGG + chr4_1:42778241-42778260 None:intergenic 40.0%
GAGTCCAATACCATCATACT+TGG - chr4_1:42778244-42778263 Msa0548590:intron 40.0%
TGGTCCAAGTATGATGGTAT+TGG + chr4_1:42778251-42778270 None:intergenic 40.0%
AATACCATCATACTTGGACC+AGG - chr4_1:42778250-42778269 Msa0548590:intron 40.0%
AGAACATTTGTGGTTTGACC+TGG + chr4_1:42778271-42778290 None:intergenic 40.0%
GCATGTAGAATGTGGCATTT+GGG + chr4_1:42778315-42778334 None:intergenic 40.0%
AGCATGTAGAATGTGGCATT+TGG + chr4_1:42778316-42778335 None:intergenic 40.0%
TCTAGCTAGCATGTAGAATG+TGG + chr4_1:42778323-42778342 None:intergenic 40.0%
CTAGCTAGACCATATTTCAC+AGG - chr4_1:42778334-42778353 Msa0548590:intron 40.0%
!!! CAAGTTTGGTTTTTTGGGCA+TGG + chr4_1:42778595-42778614 None:intergenic 40.0%
!! TGTTGGAGAAGTGCTATAGA+AGG + chr4_1:42778685-42778704 None:intergenic 40.0%
! ATTTTCCGGTTGTACCGTTA+AGG - chr4_1:42778740-42778759 Msa0548590:CDS 40.0%
AGTCATCCTTTGCATCTTCA+TGG - chr4_1:42778889-42778908 Msa0548590:intron 40.0%
ATCTTGTTGATCCTGTTGAG+AGG - chr4_1:42778980-42778999 Msa0548590:intron 40.0%
ACAATCCAGGTGATTGACTA+TGG - chr4_1:42779055-42779074 Msa0548590:CDS 40.0%
GTATGGTTGAAACAGGTGTT+TGG - chr4_1:42779471-42779490 Msa0548590:intron 40.0%
AACTAAGGTGCACATCAAAG+TGG + chr4_1:42779508-42779527 None:intergenic 40.0%
ACTTTGATGTGCACCTTAGT+TGG - chr4_1:42779507-42779526 Msa0548590:intron 40.0%
CTTTGATGTGCACCTTAGTT+GGG - chr4_1:42779508-42779527 Msa0548590:intron 40.0%
TTTGATGTGCACCTTAGTTG+GGG - chr4_1:42779509-42779528 Msa0548590:intron 40.0%
!! GGAGGAGGCAATTTTTGATT+AGG + chr4_1:42779572-42779591 None:intergenic 40.0%
GGAAAATTGGCAGAGTCAAT+TGG + chr4_1:42779628-42779647 None:intergenic 40.0%
GACCAAAAAATAAAGGTGGG+TGG - chr4_1:42779840-42779859 Msa0548590:CDS 40.0%
!! AACCTCCCCTGGATTTGTTT+TGG - chr4_1:42776296-42776315 Msa0548590:three_prime_UTR 45.0%
TCTGCAGATAAGGTTGGCAA+AGG - chr4_1:42776504-42776523 Msa0548590:three_prime_UTR 45.0%
TAAGGTTGGCAAAGGTTACG+AGG - chr4_1:42776512-42776531 Msa0548590:three_prime_UTR 45.0%
!! CGGTAACCATGCTTTTTGTG+TGG + chr4_1:42776543-42776562 None:intergenic 45.0%
CCTTCTCGTACAACAATTCG+AGG + chr4_1:42776586-42776605 None:intergenic 45.0%
CCTCGAATTGTTGTACGAGA+AGG - chr4_1:42776583-42776602 Msa0548590:CDS 45.0%
ACGAGAAGGGGACAGATTAT+TGG - chr4_1:42776597-42776616 Msa0548590:CDS 45.0%
TGAGACAACTTCGAAGTGGT+TGG - chr4_1:42776969-42776988 Msa0548590:intron 45.0%
ACAACTTCGAAGTGGTTGGT+CGG - chr4_1:42776973-42776992 Msa0548590:intron 45.0%
GACAAAGAGGAACTCTCTTC+TGG - chr4_1:42777068-42777087 Msa0548590:intron 45.0%
TCTGGCATGCTCATTTCTCA+TGG - chr4_1:42777086-42777105 Msa0548590:intron 45.0%
ATTCATTGTGCCTTGGGAGA+AGG + chr4_1:42777141-42777160 None:intergenic 45.0%
AGAATGGGGACTTCCTTGTA+GGG + chr4_1:42777181-42777200 None:intergenic 45.0%
AAGGAAGTCCCCATTCTCTT+TGG - chr4_1:42777184-42777203 Msa0548590:intron 45.0%
GCTTGTGCAATTACAGCTTC+AGG + chr4_1:42777426-42777445 None:intergenic 45.0%
AGGTCCAGGAAACCCATTAA+TGG + chr4_1:42777490-42777509 None:intergenic 45.0%
GAGCAATTGTAAAGAGGTCC+AGG + chr4_1:42777504-42777523 None:intergenic 45.0%
AAGATGCATCTGCTTCGACA+AGG + chr4_1:42778210-42778229 None:intergenic 45.0%
TTGACCTGGTCCAAGTATGA+TGG + chr4_1:42778257-42778276 None:intergenic 45.0%
TAGACCATATTTCACAGGGC+AGG - chr4_1:42778339-42778358 Msa0548590:intron 45.0%
AGACCATATTTCACAGGGCA+GGG - chr4_1:42778340-42778359 Msa0548590:intron 45.0%
TTCGACAATTCCACAGTAGC+TGG - chr4_1:42778367-42778386 Msa0548590:intron 45.0%
!! GGCACATTTGCAGGGTATTT+TGG + chr4_1:42778523-42778542 None:intergenic 45.0%
!! TGGACTAGTTCCTAGTCCAA+CGG + chr4_1:42778575-42778594 None:intergenic 45.0%
GCAACAGTATTCCTCTCAAC+AGG + chr4_1:42778994-42779013 None:intergenic 45.0%
AATACTGTTGCTGTGCCTTC+TGG - chr4_1:42779003-42779022 Msa0548590:CDS 45.0%
AATCACCTGGATTGTCAGCA+AGG + chr4_1:42779050-42779069 None:intergenic 45.0%
CAGAACCATAGTCAATCACC+TGG + chr4_1:42779063-42779082 None:intergenic 45.0%
! ATGGCTTGGATTGTTGAGGA+TGG - chr4_1:42779539-42779558 Msa0548590:intron 45.0%
AAAATTGCCTCCTCCTCCTA+AGG - chr4_1:42779577-42779596 Msa0548590:intron 45.0%
CAACATTTGGGGAGATCCTT+AGG + chr4_1:42779596-42779615 None:intergenic 45.0%
!! TAAAATATTATTAATTAAAG+TGG + chr4_1:42777879-42777898 None:intergenic 5.0%
CAAATCCAGGGGAGGTTAAG+AGG + chr4_1:42776293-42776312 None:intergenic 50.0%
AGCCAAAACAAATCCAGGGG+AGG + chr4_1:42776301-42776320 None:intergenic 50.0%
GCTGTGCCACACAAAAAGCA+TGG - chr4_1:42776534-42776553 Msa0548590:three_prime_UTR 50.0%
TACCGTGAATGGGAAGTTCC+CGG - chr4_1:42776558-42776577 Msa0548590:three_prime_UTR 50.0%
GTACAACAATTCGAGGACCC+GGG + chr4_1:42776579-42776598 None:intergenic 50.0%
CGTACAACAATTCGAGGACC+CGG + chr4_1:42776580-42776599 None:intergenic 50.0%
GGTGTGAGACAACTTCGAAG+TGG - chr4_1:42776965-42776984 Msa0548590:intron 50.0%
GAGAATGGGGACTTCCTTGT+AGG + chr4_1:42777182-42777201 None:intergenic 50.0%
ATTGCACAAGCTCTGCAGAC+AGG - chr4_1:42777435-42777454 Msa0548590:CDS 50.0%
TTGCACAAGCTCTGCAGACA+GGG - chr4_1:42777436-42777455 Msa0548590:CDS 50.0%
ACAAGCTGCACGTGTACCTT+TGG + chr4_1:42777526-42777545 None:intergenic 50.0%
GTACCCTGCCCTGTGAAATA+TGG + chr4_1:42778346-42778365 None:intergenic 50.0%
! TCATTGATGGCTGGAAGCAC+AGG + chr4_1:42778451-42778470 None:intergenic 50.0%
AACGGCCTTAACGGTACAAC+CGG + chr4_1:42778748-42778767 None:intergenic 50.0%
CGATTCCTTGCTGACAATCC+AGG - chr4_1:42779042-42779061 Msa0548590:CDS 50.0%
TGCACCTTAGTTGGGGATTG+AGG - chr4_1:42779516-42779535 Msa0548590:intron 50.0%
CCATCCTCAATCCCCAACTA+AGG + chr4_1:42779523-42779542 None:intergenic 50.0%
CCTTAGTTGGGGATTGAGGA+TGG - chr4_1:42779520-42779539 Msa0548590:intron 50.0%
! GAGGATGGCTTGGATTGTTG+AGG - chr4_1:42779535-42779554 Msa0548590:intron 50.0%
CATTTGGGGAGATCCTTAGG+AGG + chr4_1:42779593-42779612 None:intergenic 50.0%
TGCAGAGAGTGAAGTGTGTC+CGG - chr4_1:42779749-42779768 Msa0548590:intron 50.0%
AGGGGAGGTTAAGAGGAAGG+TGG + chr4_1:42776286-42776305 None:intergenic 55.0%
ACCTTCCTCTTAACCTCCCC+TGG - chr4_1:42776285-42776304 Msa0548590:three_prime_UTR 55.0%
TCCAGGGGAGGTTAAGAGGA+AGG + chr4_1:42776289-42776308 None:intergenic 55.0%
ACCGTGAATGGGAAGTTCCC+GGG - chr4_1:42776559-42776578 Msa0548590:three_prime_UTR 55.0%
ACCCGGGAACTTCCCATTCA+CGG + chr4_1:42776563-42776582 None:intergenic 55.0%
! CTTCGAAGTGGTTGGTCGGA+TGG - chr4_1:42776977-42776996 Msa0548590:intron 55.0%
GACCTCTCATCCTTCTCCCA+AGG - chr4_1:42777128-42777147 Msa0548590:intron 55.0%
TGCCTTGGGAGAAGGATGAG+AGG + chr4_1:42777133-42777152 None:intergenic 55.0%
TGCACAAGCTCTGCAGACAG+GGG - chr4_1:42777437-42777456 Msa0548590:CDS 55.0%
ACTGTTGCTGTGCCTTCTGG+TGG - chr4_1:42779006-42779025 Msa0548590:CDS 55.0%
TTGCTGTGCCTTCTGGTGGT+TGG - chr4_1:42779010-42779029 Msa0548590:CDS 55.0%
TGCTGTGCCTTCTGGTGGTT+GGG - chr4_1:42779011-42779030 Msa0548590:CDS 55.0%
GTTGGGGATTGAGGATGGCT+TGG - chr4_1:42779525-42779544 Msa0548590:intron 55.0%
TTGGGGAGATCCTTAGGAGG+AGG + chr4_1:42779590-42779609 None:intergenic 55.0%
GCACAAGCTCTGCAGACAGG+GGG - chr4_1:42777438-42777457 Msa0548590:CDS 60.0%
ATCGCGACCCAACCACCAGA+AGG + chr4_1:42779021-42779040 None:intergenic 60.0%
GGGAGATCCTTAGGAGGAGG+AGG + chr4_1:42779587-42779606 None:intergenic 60.0%
CAAGCTCTGCAGACAGGGGG+TGG - chr4_1:42777441-42777460 Msa0548590:CDS 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 42776158 42780040 42776158 ID=Msa0548590;Name=Msa0548590
chr4_1 mRNA 42776158 42780040 42776158 ID=Msa0548590-mRNA-1;Parent=Msa0548590;Name=Msa0548590-mRNA-1;_AED=0.07;_eAED=0.10;_QI=58|0.4|0.66|1|0.6|0.66|6|424|516
chr4_1 exon 42776158 42776712 42776158 ID=Msa0548590-mRNA-1:exon:11040;Parent=Msa0548590-mRNA-1
chr4_1 exon 42777261 42778097 42777261 ID=Msa0548590-mRNA-1:exon:11039;Parent=Msa0548590-mRNA-1
chr4_1 exon 42778670 42778795 42778670 ID=Msa0548590-mRNA-1:exon:11038;Parent=Msa0548590-mRNA-1
chr4_1 exon 42778993 42779237 42778993 ID=Msa0548590-mRNA-1:exon:11037;Parent=Msa0548590-mRNA-1
chr4_1 exon 42779618 42779682 42779618 ID=Msa0548590-mRNA-1:exon:11036;Parent=Msa0548590-mRNA-1
chr4_1 exon 42779836 42780040 42779836 ID=Msa0548590-mRNA-1:exon:11035;Parent=Msa0548590-mRNA-1
chr4_1 five_prime_UTR 42779983 42780040 42779983 ID=Msa0548590-mRNA-1:five_prime_utr;Parent=Msa0548590-mRNA-1
chr4_1 CDS 42779836 42779982 42779836 ID=Msa0548590-mRNA-1:cds;Parent=Msa0548590-mRNA-1
chr4_1 CDS 42779618 42779682 42779618 ID=Msa0548590-mRNA-1:cds;Parent=Msa0548590-mRNA-1
chr4_1 CDS 42778993 42779237 42778993 ID=Msa0548590-mRNA-1:cds;Parent=Msa0548590-mRNA-1
chr4_1 CDS 42778670 42778795 42778670 ID=Msa0548590-mRNA-1:cds;Parent=Msa0548590-mRNA-1
chr4_1 CDS 42777261 42778097 42777261 ID=Msa0548590-mRNA-1:cds;Parent=Msa0548590-mRNA-1
chr4_1 CDS 42776582 42776712 42776582 ID=Msa0548590-mRNA-1:cds;Parent=Msa0548590-mRNA-1
chr4_1 three_prime_UTR 42776158 42776581 42776158 ID=Msa0548590-mRNA-1:three_prime_utr;Parent=Msa0548590-mRNA-1
Gene Sequence

>Msa0548590

ATGCATATCAAAATGATTGTCACTGTTCCATTATCAAAGGCTCTTTTTGCAGCTTTTCTCTTTGTCACCACCTTCCTCTTAACCTCCCCTGGATTTGTTTTGGCTACTTCACAACATGGAAGTGTTACAAGACACTACAAATTTGATGTTGGCAAAGGTTACGAGGCTGTGCCACACAAAAAGCATGGTTACCGTGAATGGGAAGTTCCCGGGCATGGTGTGAGACAACTTCGAAGTGGTTGGTCGGATGGTCCATCATACATAACTCAATGCCCAATTCAAACAGGTCAGAGTTATGTGTACAACTTCACCATTGTTGGACAAAGAGGAACTCTCTTCTGGCATGCTCATTTCTCATGGTTAAGAGCTACTGTCTATGGACCTCTCATCCTTCTCCCAAGGCACAATGAATCTTATCCTTTTCAAAAACCCTACAAGGAAGTCCCCATTCTCTTTGGTGAGTGGTGGAATGCAGATCCTGAAGCTGTAATTGCACAAGCTCTGCAGACAGGGGGTGGCCCAAATGTTTCTGATGCCTACACCATTAATGGGTTTCCTGGACCTCTTTACAATTGCTCCAAAGATACATACAAATTGAAGGTGAAGCCAGGAAAAACATATCTATTACGTTTGATCAACGCTGCACTCAACGACGAACTCTTTTTCAGCATTGCTAATCACACATTAATCCTTGTCGAAGCAGATGCATCTTACATTAAACCATTCGAGTCCAATACCATCATACTTGGACCAGGTCAAACCACAAATGTTCTATTAAAAACTAAACCAAATTACCCAAATGCCACATTCTACATGCTAGCTAGACCATATTTCACAGGGCAGGGTACTTTCGACAATTCCACAGTAGCTGGAATTTTAGAATACACTAAACCAAATGATCAAACAAATAAAAATCTCCATATTCTAAAACCTGTGCTTCCAGCCATCAATGATACTAATTTTGTTGCTAATTTTAGTAACAAATTTCTTAGCTTAAATAGTCCAAAATACCCTGCAAATGTGCCTAAAACTATTGACAAAAACTTTTTCTTTACCGTTGGACTAGGAACTAGTCCATGCCCAAAAAACCAAACTTGTCAAGGACCAAATAATAGTTCAAATTCAAAATTTGCAGCTTCAATGAACAATGTATCATTTGCTTTACCTTCTATAGCACTTCTCCAACAACATTTCTTTGGAACTAATAATGGTTTTACAACGGATTTTCCGGTTGTACCGTTAAGGCCGTTTAACTATACAGGAACTCCTCCAAACAATACTTTGGTTAGTAATGGTACAAAGACAGTGGTGATACCATATAACACTAGAGTTCAGGTTATATTGCAGGATACAAGCATTTTGGGAGCTGAGAGTCATCCTTTGCATCTTCATGGATTTAATTTCTTTGTTGTTGGTCAAGGTGTTTGGCTGATGCATTGCCACTTTGATGTGCACCTTAGTTGGGGATTGAGGATGGCTTGGATTGTTGAGGATGGAAAGTTACCTAATCAAAAATTGCCTCCTCCTCCTAAGGATCTCCCCAAATGTTGA
Protein sequence

>Msa0548590

MHIKMIVTVPLSKALFAAFLFVTTFLLTSPGFVLATSQHGSVTRHYKFDVGKGYEAVPHKKHGYREWEVPGHGVRQLRSGWSDGPSYITQCPIQTGQSYVYNFTIVGQRGTLFWHAHFSWLRATVYGPLILLPRHNESYPFQKPYKEVPILFGEWWNADPEAVIAQALQTGGGPNVSDAYTINGFPGPLYNCSKDTYKLKVKPGKTYLLRLINAALNDELFFSIANHTLILVEADASYIKPFESNTIILGPGQTTNVLLKTKPNYPNATFYMLARPYFTGQGTFDNSTVAGILEYTKPNDQTNKNLHILKPVLPAINDTNFVANFSNKFLSLNSPKYPANVPKTIDKNFFFTVGLGTSPCPKNQTCQGPNNSSNSKFAASMNNVSFALPSIALLQQHFFGTNNGFTTDFPVVPLRPFNYTGTPPNNTLVSNGTKTVVIPYNTRVQVILQDTSILGAESHPLHLHGFNFFVVGQGVWLMHCHFDVHLSWGLRMAWIVEDGKLPNQKLPPPPKDLPKC*