AlfalfaGEDB | Msa0549530

M. sativa cultivar zhongmu-4 / Msa0549530

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0549530	AES89058.1	90.667	75	6	1	1	75	1	74	2.13e-39	134

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0549530	G7JF00	90.667	75	6	1	1	75	1	74	1.02e-39	134

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0216370	Msa0549530	0.819690	1.017344e-52	-8.615850e-47
Msa0256570	Msa0549530	0.838443	2.866621e-57	-8.615850e-47
Msa0295710	Msa0549530	0.841660	4.153532e-58	-8.615850e-47
Msa0334300	Msa0549530	0.845028	5.241630e-59	-8.615850e-47
Msa0549530	Msa0645880	0.892882	1.052799e-74	-8.615850e-47
Msa0549530	Msa0685730	0.928640	2.169331e-92	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0549530	MtrunA17_Chr4g0034081	90.667	75	6	1	1	75	1	74	1.96e-43	134
Msa0549530	MtrunA17_Chr4g0034091	70.667	75	18	2	1	75	1	71	7.63e-30	100
Msa0549530	MtrunA17_Chr1g0162551	52.000	75	31	3	1	74	1	71	7.84e-19	72.8
Msa0549530	MtrunA17_Chr1g0162491	44.595	74	33	1	1	74	1	66	9.76e-17	67.4

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0549530	AT2G15960.1	40.244	82	37	2	1	75	1	77	8.81e-14	60.5

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Find 20 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAAAGGCTTGCTGTTGCT+AGG	0.594023	4_1:+44753556	None:intergenic
AGGCAACAGAGGTGTCTCTT+GGG	0.412772	4_1:+44753476	None:intergenic
ATTAACATATACTATCACCA+AGG	0.710327	4_1:+44753397	None:intergenic
ATTAATGGCTGCCATTGTAG+AGG	0.614505	4_1:-44753620	None:intergenic
CAAAACAAGAAGAAGAACGT+TGG	0.651156	4_1:-44753508	Msa0549530:CDS
CATTGTAGAGGTATGGGTTG+GGG	0.470237	4_1:-44753608	Msa0549530:CDS
CCATTGTAGAGGTATGGGTT+GGG	0.390310	4_1:-44753609	Msa0549530:CDS
CCCAACCCATACCTCTACAA+TGG	0.629283	4_1:+44753609	None:intergenic
GAGGCAACAGAGGTGTCTCT+TGG	0.459401	4_1:+44753475	None:intergenic
GCAACAGAGGTGTCTCTTGG+GGG	0.674229	4_1:+44753478	None:intergenic

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CRISPR-GE

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badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	CAAAACAAGAAGAAGAACGT+TGG	-	chr4_1:44753519-44753538	Msa0549530:CDS	35.0%
	TGACATAGTTGAAGAGTCAG+AGG	+	chr4_1:44753574-44753593	None:intergenic	40.0%
	TGACTCTTCAACTATGTCAG+AGG	-	chr4_1:44753575-44753594	Msa0549530:CDS	40.0%
	TCGATCCATAAGCAAACACA+CGG	+	chr4_1:44753604-44753623	None:intergenic	40.0%
	TGGCTGCCATTGTAGAGGTA+TGG	-	chr4_1:44753412-44753431	Msa0549530:CDS	50.0%
	CCCAACCCATACCTCTACAA+TGG	+	chr4_1:44753421-44753440	None:intergenic	50.0%
	TGAAGAGTCAGAGGCAACAG+AGG	+	chr4_1:44753565-44753584	None:intergenic	50.0%
	GGCAACCGTGTGTTTGCTTA+TGG	-	chr4_1:44753596-44753615	Msa0549530:CDS	50.0%
!	TTGTTTTCAAAAGCCAAACA+AGG	-	chr4_1:44753489-44753508	Msa0549530:CDS	30.0%
!	CCATTGTAGAGGTATGGGTT+GGG	-	chr4_1:44753418-44753437	Msa0549530:CDS	45.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_1	gene	44753411	44753638	44753411	ID=Msa0549530;Name=Msa0549530
chr4_1	mRNA	44753411	44753638	44753411	ID=Msa0549530-mRNA-1;Parent=Msa0549530;Name=Msa0549530-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|75
chr4_1	exon	44753411	44753638	44753411	ID=Msa0549530-mRNA-1:exon:11686;Parent=Msa0549530-mRNA-1
chr4_1	CDS	44753411	44753638	44753411	ID=Msa0549530-mRNA-1:cds;Parent=Msa0549530-mRNA-1

Gene Sequence	>Msa0549530 ATGGCTGCCATTGTAGAGGTATGGGTTGGGGAGCTAACAAAGCTCAGAGAAAAAGTTCTTCCTAGCAACAGCAAGCCTTTGTTTTCAAAAGCCAAACAAGGCGCAGATCAAAACAAGAAGAAGAACGTTGGTGTTCTTCCCCCAAGAGACACCTCTGTTGCCTCTGACTCTTCAACTATGTCAGAGGCAACCGTGTGTTTGCTTATGGATCGATTTGTTCCTTGGTGA
Protein sequence	>Msa0549530 MAAIVEVWVGELTKLREKVLPSNSKPLFSKAKQGADQNKKKNVGVLPPRDTSVASDSSTMSEATVCLLMDRFVPW*