Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0549530 | AES89058.1 | 90.667 | 75 | 6 | 1 | 1 | 75 | 1 | 74 | 2.13e-39 | 134 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0549530 | G7JF00 | 90.667 | 75 | 6 | 1 | 1 | 75 | 1 | 74 | 1.02e-39 | 134 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0216370 | Msa0549530 | 0.819690 | 1.017344e-52 | -8.615850e-47 |
| Msa0256570 | Msa0549530 | 0.838443 | 2.866621e-57 | -8.615850e-47 |
| Msa0295710 | Msa0549530 | 0.841660 | 4.153532e-58 | -8.615850e-47 |
| Msa0334300 | Msa0549530 | 0.845028 | 5.241630e-59 | -8.615850e-47 |
| Msa0549530 | Msa0645880 | 0.892882 | 1.052799e-74 | -8.615850e-47 |
| Msa0549530 | Msa0685730 | 0.928640 | 2.169331e-92 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0549530 | MtrunA17_Chr4g0034081 | 90.667 | 75 | 6 | 1 | 1 | 75 | 1 | 74 | 1.96e-43 | 134 |
| Msa0549530 | MtrunA17_Chr4g0034091 | 70.667 | 75 | 18 | 2 | 1 | 75 | 1 | 71 | 7.63e-30 | 100 |
| Msa0549530 | MtrunA17_Chr1g0162551 | 52.000 | 75 | 31 | 3 | 1 | 74 | 1 | 71 | 7.84e-19 | 72.8 |
| Msa0549530 | MtrunA17_Chr1g0162491 | 44.595 | 74 | 33 | 1 | 1 | 74 | 1 | 66 | 9.76e-17 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0549530 | AT2G15960.1 | 40.244 | 82 | 37 | 2 | 1 | 75 | 1 | 77 | 8.81e-14 | 60.5 |
Find 20 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCAACCGTGTGTTTGCTTA+TGG | 0.279387 | 4_1:-44753431 | Msa0549530:CDS |
| CCATTGTAGAGGTATGGGTT+GGG | 0.390310 | 4_1:-44753609 | Msa0549530:CDS |
| AGGCAACAGAGGTGTCTCTT+GGG | 0.412772 | 4_1:+44753476 | None:intergenic |
| TTATGGATCGATTTGTTCCT+TGG | 0.422668 | 4_1:-44753414 | Msa0549530:CDS |
| TGGCTGCCATTGTAGAGGTA+TGG | 0.433651 | 4_1:-44753615 | Msa0549530:CDS |
| GAGGCAACAGAGGTGTCTCT+TGG | 0.459401 | 4_1:+44753475 | None:intergenic |
| CATTGTAGAGGTATGGGTTG+GGG | 0.470237 | 4_1:-44753608 | Msa0549530:CDS |
| GCCATTGTAGAGGTATGGGT+TGG | 0.477069 | 4_1:-44753610 | Msa0549530:CDS |
| GGCAACAGAGGTGTCTCTTG+GGG | 0.546169 | 4_1:+44753477 | None:intergenic |
| GGCTGCCATTGTAGAGGTAT+GGG | 0.559523 | 4_1:-44753614 | Msa0549530:CDS |
| AACAAAGGCTTGCTGTTGCT+AGG | 0.594023 | 4_1:+44753556 | None:intergenic |
| TGAAGAGTCAGAGGCAACAG+AGG | 0.597712 | 4_1:+44753465 | None:intergenic |
| ATTAATGGCTGCCATTGTAG+AGG | 0.614505 | 4_1:-44753620 | None:intergenic |
| TGACATAGTTGAAGAGTCAG+AGG | 0.626699 | 4_1:+44753456 | None:intergenic |
| CCCAACCCATACCTCTACAA+TGG | 0.629283 | 4_1:+44753609 | None:intergenic |
| CAAAACAAGAAGAAGAACGT+TGG | 0.651156 | 4_1:-44753508 | Msa0549530:CDS |
| TGACTCTTCAACTATGTCAG+AGG | 0.654740 | 4_1:-44753452 | Msa0549530:CDS |
| GCAACAGAGGTGTCTCTTGG+GGG | 0.674229 | 4_1:+44753478 | None:intergenic |
| ATTAACATATACTATCACCA+AGG | 0.710327 | 4_1:+44753397 | None:intergenic |
| TCGATCCATAAGCAAACACA+CGG | 0.710548 | 4_1:+44753426 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGTTTGGCTTTTGAAAACAA+AGG | + | chr4_1:44753489-44753508 | None:intergenic | 30.0% |
| ! | TTGTTTTCAAAAGCCAAACA+AGG | - | chr4_1:44753489-44753508 | Msa0549530:CDS | 30.0% |
| CAAAACAAGAAGAAGAACGT+TGG | - | chr4_1:44753519-44753538 | Msa0549530:CDS | 35.0% | |
| !! | TTATGGATCGATTTGTTCCT+TGG | - | chr4_1:44753613-44753632 | Msa0549530:CDS | 35.0% |
| TGACATAGTTGAAGAGTCAG+AGG | + | chr4_1:44753574-44753593 | None:intergenic | 40.0% | |
| TGACTCTTCAACTATGTCAG+AGG | - | chr4_1:44753575-44753594 | Msa0549530:CDS | 40.0% | |
| TCGATCCATAAGCAAACACA+CGG | + | chr4_1:44753604-44753623 | None:intergenic | 40.0% | |
| ! | CCATTGTAGAGGTATGGGTT+GGG | - | chr4_1:44753418-44753437 | Msa0549530:CDS | 45.0% |
| ! | CATTGTAGAGGTATGGGTTG+GGG | - | chr4_1:44753419-44753438 | Msa0549530:CDS | 45.0% |
| !! | AACAAAGGCTTGCTGTTGCT+AGG | + | chr4_1:44753474-44753493 | None:intergenic | 45.0% |
| !! | GTTTTGATCTGCGCCTTGTT+TGG | + | chr4_1:44753505-44753524 | None:intergenic | 45.0% |
| TGGCTGCCATTGTAGAGGTA+TGG | - | chr4_1:44753412-44753431 | Msa0549530:CDS | 50.0% | |
| ! | GGCTGCCATTGTAGAGGTAT+GGG | - | chr4_1:44753413-44753432 | Msa0549530:CDS | 50.0% |
| ! | GCCATTGTAGAGGTATGGGT+TGG | - | chr4_1:44753417-44753436 | Msa0549530:CDS | 50.0% |
| CCCAACCCATACCTCTACAA+TGG | + | chr4_1:44753421-44753440 | None:intergenic | 50.0% | |
| !! | AGGCAACAGAGGTGTCTCTT+GGG | + | chr4_1:44753554-44753573 | None:intergenic | 50.0% |
| TGAAGAGTCAGAGGCAACAG+AGG | + | chr4_1:44753565-44753584 | None:intergenic | 50.0% | |
| GGCAACCGTGTGTTTGCTTA+TGG | - | chr4_1:44753596-44753615 | Msa0549530:CDS | 50.0% | |
| !! | GCAACAGAGGTGTCTCTTGG+GGG | + | chr4_1:44753552-44753571 | None:intergenic | 55.0% |
| !! | GGCAACAGAGGTGTCTCTTG+GGG | + | chr4_1:44753553-44753572 | None:intergenic | 55.0% |
| !! | GAGGCAACAGAGGTGTCTCT+TGG | + | chr4_1:44753555-44753574 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_1 | gene | 44753411 | 44753638 | 44753411 | ID=Msa0549530;Name=Msa0549530 |
| chr4_1 | mRNA | 44753411 | 44753638 | 44753411 | ID=Msa0549530-mRNA-1;Parent=Msa0549530;Name=Msa0549530-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0|-1|0|1|-1|1|1|0|75 |
| chr4_1 | exon | 44753411 | 44753638 | 44753411 | ID=Msa0549530-mRNA-1:exon:11686;Parent=Msa0549530-mRNA-1 |
| chr4_1 | CDS | 44753411 | 44753638 | 44753411 | ID=Msa0549530-mRNA-1:cds;Parent=Msa0549530-mRNA-1 |
| Gene Sequence |
| Protein sequence |
