AlfalfaGEDB | Msa0256570

M. sativa cultivar zhongmu-4 / Msa0256570

KEGG: -
GO: -
PFAM: PF12481.11

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0256570	XP_013464732.1	96.838	253	8	0	1	253	1	253	0.0	511

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0256570	sp\|P24805\|TSJT1_TOBAC	40.940	149	87	1	1	149	1	148	2.13e-32	119

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0256570	B7FHV5	96.838	253	8	0	1	253	1	253	0.0	511

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0186540	Msa0256570	0.844345	8.008092e-59	-8.615850e-47
Msa0216370	Msa0256570	0.892144	2.080321e-74	-8.615850e-47
Msa0225420	Msa0256570	0.819957	8.840130e-53	-8.615850e-47
Msa0225720	Msa0256570	0.815544	8.756625e-52	-8.615850e-47
Msa0256570	Msa0295710	0.977150	3.296953e-143	-8.615850e-47
Msa0256570	Msa0301260	0.832772	7.811974e-56	-8.615850e-47
Msa0256570	Msa0334300	0.960429	1.510056e-118	-8.615850e-47
Msa0256570	Msa0385740	0.854775	9.834865e-62	-8.615850e-47
Msa0256570	Msa0420830	0.804847	1.774611e-49	-8.615850e-47
Msa0256570	Msa0549530	0.838443	2.866621e-57	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0256570	MtrunA17_Chr2g0317901	96.838	253	8	0	1	253	1	253	0.0	511
Msa0256570	MtrunA17_Chr2g0281901	65.600	250	85	1	1	250	1	249	8.34e-116	332
Msa0256570	MtrunA17_Chr4g0047531	60.558	251	98	1	1	250	64	314	1.57e-105	308
Msa0256570	MtrunA17_Chr1g0166351	47.012	251	124	4	1	247	1	246	5.10e-76	231
Msa0256570	MtrunA17_Chr1g0204611	47.222	252	122	5	1	247	1	246	1.12e-75	230
Msa0256570	MtrunA17_Chr2g0287871	40.948	232	130	5	1	230	1	227	1.38e-53	173
Msa0256570	MtrunA17_Chr4g0065571	39.056	233	134	6	1	230	1	228	7.52e-53	171

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0256570	AT4G27450.1	78.226	248	53	1	1	247	1	248	8.92e-146	408
Msa0256570	AT3G15450.1	68.675	249	74	3	1	247	1	247	2.31e-124	353
Msa0256570	AT3G15450.2	66.839	193	60	3	1	191	1	191	3.99e-88	260
Msa0256570	AT5G43830.1	48.413	252	120	4	1	247	1	247	1.72e-78	237
Msa0256570	AT3G15450.3	64.286	182	58	4	1	180	1	177	2.46e-74	224
Msa0256570	AT3G22850.1	44.939	247	133	1	1	247	1	244	5.28e-73	223
Msa0256570	AT5G19140.1	42.241	232	127	5	1	230	1	227	3.31e-55	177
Msa0256570	AT5G19140.3	48.521	169	84	2	63	230	25	191	5.81e-50	163
Msa0256570	AT5G19140.2	39.655	232	121	6	1	230	1	215	3.45e-47	156

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Find 77 sgRNAs with CRISPR-Local

Find 258 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGATAAGGTAAGGTAGG+TGG	0.633343	2_2:-51407284	None:intergenic
AAATTCAAAAGGATAAGGTA+AGG	0.599797	2_2:-51407291	None:intergenic
AACCCTCTTGTACAACATTA+AGG	0.386014	2_2:-51406558	None:intergenic
AACCTTTGATGAAGAGAAAA+AGG	0.230736	2_2:-51405691	None:intergenic
AAGCAATATGGTTTGTCAAA+GGG	0.505741	2_2:+51406220	Msa0256570:CDS
AAGGTAAGGTAGGTGGTGGT+AGG	0.464791	2_2:-51407277	None:intergenic
AAGGTAGGTGGTGGTAGGGT+TGG	0.534755	2_2:-51407272	None:intergenic
AATACTTGTTCTATGAACTT+TGG	0.381957	2_2:+51405634	Msa0256570:CDS
AATAGCCCTGCATCATACAA+AGG	0.630008	2_2:+51405550	Msa0256570:CDS
ACAACTTGATCTGGTGGATA+TGG	0.300897	2_2:-51406298	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGGATTAAAAATGGAAGCA+GGG	-	chr2_2:51405414-51405433	None:intergenic	30.0%
AGAATTTCTTCAGAAAGCTT+AGG	-	chr2_2:51405586-51405605	None:intergenic	30.0%
AACCTTTGATGAAGAGAAAA+AGG	-	chr2_2:51405694-51405713	None:intergenic	30.0%
TAACATAATACACATGTCTC+TGG	-	chr2_2:51405966-51405985	None:intergenic	30.0%
ATAAGCTTCAATCAGAAACA+TGG	-	chr2_2:51406257-51406276	None:intergenic	30.0%
ATTACATCATAACCAACATG+TGG	+	chr2_2:51406678-51406697	Msa0256570:intron	30.0%
TAGATCAATATGATACGGTT+TGG	+	chr2_2:51406793-51406812	Msa0256570:intron	30.0%
CACTTCAAACAAATTGTCAA+AGG	+	chr2_2:51406945-51406964	Msa0256570:intron	30.0%
GAACTTATCAACCTTAAAGT+TGG	-	chr2_2:51407106-51407125	None:intergenic	30.0%
AGGACAAATTCAAAAGGATA+AGG	-	chr2_2:51407299-51407318	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_2	gene	51405393	51407442	51405393	ID=Msa0256570;Name=Msa0256570
chr2_2	mRNA	51405393	51407442	51405393	ID=Msa0256570-mRNA-1;Parent=Msa0256570;Name=Msa0256570-mRNA-1;_AED=0.10;_eAED=0.10;_QI=109\|1\|1\|1\|1\|1\|4\|251\|253
chr2_2	exon	51405393	51405710	51405393	ID=Msa0256570-mRNA-1:exon:15531;Parent=Msa0256570-mRNA-1
chr2_2	exon	51406150	51406390	51406150	ID=Msa0256570-mRNA-1:exon:15532;Parent=Msa0256570-mRNA-1
chr2_2	exon	51406490	51406610	51406490	ID=Msa0256570-mRNA-1:exon:15533;Parent=Msa0256570-mRNA-1
chr2_2	exon	51407001	51407442	51407001	ID=Msa0256570-mRNA-1:exon:15534;Parent=Msa0256570-mRNA-1
chr2_2	five_prime_UTR	51405393	51405501	51405393	ID=Msa0256570-mRNA-1:five_prime_utr;Parent=Msa0256570-mRNA-1
chr2_2	CDS	51405502	51405710	51405502	ID=Msa0256570-mRNA-1:cds;Parent=Msa0256570-mRNA-1
chr2_2	CDS	51406150	51406390	51406150	ID=Msa0256570-mRNA-1:cds;Parent=Msa0256570-mRNA-1
chr2_2	CDS	51406490	51406610	51406490	ID=Msa0256570-mRNA-1:cds;Parent=Msa0256570-mRNA-1
chr2_2	CDS	51407001	51407191	51407001	ID=Msa0256570-mRNA-1:cds;Parent=Msa0256570-mRNA-1
chr2_2	three_prime_UTR	51407192	51407442	51407192	ID=Msa0256570-mRNA-1:three_prime_utr;Parent=Msa0256570-mRNA-1

Gene Sequence

>Msa0256570

ATGTTGGCAATTTTCCACAAAGGTCTTGCTCATCCACCTGAAGAGCTGAATAGCCCTGCATCATACAAAGGCTTAAAGAAACCTAAGCTTTCTGAAGAAATTCTCAGAGAATTCATTTCTCATCATCCTGATAATACTTGTTCTATGAACTTTGGAAAAGCTGCTCTTGCTTATGTTAGACCTGATAAGCCTTTTTCTCTTCATCAAAGGTTGTTTTGTGGGCTTGATGACATATACAGCTTGTTCTTGGGGAGTTTGAACAATTTGTCCTTACTGAACAAGCAATATGGTTTGTCAAAGGGAACTGATGAAGCCATGTTTCTGATTGAAGCTTATAGGACACTCCGTGACCGTGGTCCATATCCACCAGATCAAGTTGTTAAGGAACTTGATGGCAGTTTTGCCTTTGTTGTTTATGACAGCACATATGGAATTGTTTTTGCTGCACTGGGTTCTGATGGTGGATTGAAGTTGTATTGGGGTATTGCCGCTGATGGGTCTGTTGTGATCTCTGATGACCTTAATGTTGTACAAGAGGGTTGTGCTAAATCCTTTGCTCCTTTTCCAGCAGGGTGTATGTTTCATAGTGAAGGAGGTTTAATGAGCTTTGAGCATCCATTGAACAAGTTGAAGGCAATGCCAAGGATAGACAGTGAAGGAGTGATGTGTGGGGCCAACTTTAAGGTTGATAAGTTCTCAAGAGTCAACAGTATTCCTCGAGTTGGTAGTGAATCCAATTGGATGGAATGGGAGCAACATTAG

Protein sequence

>Msa0256570

MLAIFHKGLAHPPEELNSPASYKGLKKPKLSEEILREFISHHPDNTCSMNFGKAALAYVRPDKPFSLHQRLFCGLDDIYSLFLGSLNNLSLLNKQYGLSKGTDEAMFLIEAYRTLRDRGPYPPDQVVKELDGSFAFVVYDSTYGIVFAALGSDGGLKLYWGIAADGSVVISDDLNVVQEGCAKSFAPFPAGCMFHSEGGLMSFEHPLNKLKAMPRIDSEGVMCGANFKVDKFSRVNSIPRVGSESNWMEWEQH*