AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1413020

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1413020 MtrunA17_Chr5g0412151 97.885 520 5 4 1 517 1 517 0.0 1046
Msa1413020 MtrunA17_Chr2g0328971 52.998 417 131 13 11 419 4 363 5.07e-126 378
Msa1413020 MtrunA17_Chr4g0072291 77.982 218 44 2 216 429 148 365 1.23e-121 366
Msa1413020 MtrunA17_Chr7g0249621 62.752 298 77 6 201 464 222 519 2.70e-119 366
Msa1413020 MtrunA17_Chr4g0032631 51.651 424 128 14 10 419 2 362 1.27e-118 360
Msa1413020 MtrunA17_Chr8g0343851 52.825 354 124 6 86 409 91 431 3.12e-111 342
Msa1413020 MtrunA17_Chr1g0152161 47.430 428 152 12 83 461 126 529 1.08e-110 343
Msa1413020 MtrunA17_Chr3g0135161 77.949 195 42 1 218 411 301 495 2.01e-108 337
Msa1413020 MtrunA17_Chr4g0054351 67.073 246 65 4 218 459 301 534 3.72e-106 331
Msa1413020 MtrunA17_Chr5g0400871 71.226 212 50 2 223 431 245 448 1.11e-103 321
Msa1413020 MtrunA17_Chr8g0367311 63.529 255 67 5 264 516 22 252 2.63e-100 302
Msa1413020 MtrunA17_Chr4g0073521 71.084 166 48 0 226 391 46 211 1.68e-82 260
Msa1413020 MtrunA17_Chr8g0355191 68.675 166 52 0 226 391 54 219 2.02e-77 249
Msa1413020 MtrunA17_Chr7g0216601 65.680 169 55 1 226 391 64 232 8.70e-75 241
Msa1413020 MtrunA17_Chr6g0452911 63.743 171 52 1 231 391 65 235 4.03e-73 237
Msa1413020 MtrunA17_Chr4g0002011 69.231 169 49 1 226 391 35 203 5.30e-72 232
Msa1413020 MtrunA17_Chr8g0355181 67.470 166 54 0 226 391 55 220 2.83e-71 232
Msa1413020 MtrunA17_Chr8g0362541 62.222 180 54 1 226 391 68 247 4.11e-66 219
Msa1413020 MtrunA17_Chr7g0257361 51.977 177 74 3 178 351 11 179 1.02e-43 160
Msa1413020 MtrunA17_Chr2g0305821 65.254 118 41 0 222 339 42 159 1.03e-43 152
Msa1413020 MtrunA17_Chr7g0263581 40.602 266 131 7 135 394 51 295 1.33e-40 152
Msa1413020 MtrunA17_Chr4g0053181 52.096 167 66 4 231 394 177 332 2.54e-40 152
Msa1413020 MtrunA17_Chr5g0401891 51.176 170 75 3 231 400 153 314 8.65e-40 150
Msa1413020 MtrunA17_Chr4g0030191 43.304 224 113 5 191 414 96 305 3.85e-39 148
Msa1413020 MtrunA17_Chr2g0325911 43.455 191 84 5 204 394 109 275 1.94e-30 123
Msa1413020 MtrunA17_Chr8g0367321 56.311 103 35 4 165 265 99 193 1.97e-24 100
Msa1413020 MtrunA17_Chr2g0305831 71.186 59 17 0 333 391 1 59 3.24e-23 97.8
Msa1413020 MtrunA17_Chr3g0126151 47.368 95 49 1 275 369 47 140 8.73e-22 91.7
Msa1413020 MtrunA17_Chr1g0170761 39.837 123 60 4 227 346 134 245 3.69e-13 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1413020 AT5G10510.6 52.961 608 154 22 12 517 7 584 3.20e-161 471
Msa1413020 AT5G10510.4 52.961 608 154 22 12 517 7 584 3.20e-161 471
Msa1413020 AT5G10510.3 51.115 628 155 22 12 517 7 604 1.11e-156 459
Msa1413020 AT5G10510.2 56.405 523 130 16 73 517 41 543 2.29e-154 451
Msa1413020 AT5G10510.1 52.349 596 158 21 18 517 4 569 8.83e-154 451
Msa1413020 AT5G65510.2 52.468 547 164 15 12 517 7 498 1.83e-153 447
Msa1413020 AT5G65510.1 52.468 547 164 15 12 517 7 498 1.83e-153 447
Msa1413020 AT5G10510.7 56.084 526 130 17 73 517 41 546 2.81e-152 446
Msa1413020 AT5G10510.5 56.084 526 130 17 73 517 41 546 2.81e-152 446
Msa1413020 AT5G65510.4 56.624 468 121 12 86 517 19 440 1.51e-148 433
Msa1413020 AT1G51190.1 51.887 424 148 15 11 419 4 386 2.94e-124 375
Msa1413020 AT3G20840.1 52.506 419 144 12 11 419 4 377 1.84e-122 371
Msa1413020 AT5G57390.2 79.512 205 39 1 208 409 63 267 1.31e-118 356
Msa1413020 AT5G57390.1 79.512 205 39 1 208 409 181 385 2.15e-117 357
Msa1413020 AT5G17430.2 67.829 258 77 3 203 456 123 378 4.32e-117 355
Msa1413020 AT5G17430.1 67.829 258 77 3 203 456 185 440 9.32e-117 357
Msa1413020 AT5G65510.3 59.344 305 85 6 83 361 16 307 7.27e-108 324
Msa1413020 AT5G65510.3 35.644 101 50 2 302 394 153 246 2.25e-11 65.5
Msa1413020 AT1G72570.1 74.500 200 48 1 207 403 200 399 6.93e-106 323
Msa1413020 AT4G37750.1 60.223 269 102 2 187 452 240 506 4.72e-105 325
Msa1413020 AT3G54320.3 60.591 203 73 3 226 422 61 262 2.46e-70 231
Msa1413020 AT3G54320.1 60.591 203 73 3 226 422 61 262 2.91e-70 231
Msa1413020 AT1G79700.2 67.456 169 52 1 226 391 48 216 2.36e-61 204
Msa1413020 AT1G16060.1 62.032 187 68 1 208 391 36 222 2.45e-61 205
Msa1413020 AT1G79700.1 63.314 169 49 2 226 391 48 206 1.91e-53 183
Msa1413020 AT3G54320.4 59.877 162 61 2 264 422 36 196 6.40e-52 181
Msa1413020 AT3G54320.5 59.877 162 61 2 264 422 36 196 6.40e-52 181
Msa1413020 AT1G79700.4 67.969 128 41 0 264 391 7 134 2.06e-42 151
Msa1413020 AT1G79700.3 67.969 128 41 0 264 391 13 140 2.11e-42 151
Msa1413020 AT2G41710.1 50.270 185 63 5 217 381 57 232 2.67e-42 156
Msa1413020 AT4G36920.1 46.377 207 103 3 194 400 95 293 3.51e-42 156
Msa1413020 AT4G36920.2 46.377 207 103 3 194 400 95 293 3.51e-42 156
Msa1413020 AT2G41710.4 50.270 185 63 5 217 381 57 232 5.53e-42 156
Msa1413020 AT1G16060.3 67.188 128 42 0 264 391 25 152 5.56e-41 149
Msa1413020 AT2G41710.2 48.947 190 63 6 217 381 57 237 6.52e-40 150
Msa1413020 AT5G67180.2 49.112 169 78 3 231 399 97 257 5.54e-39 144
Msa1413020 AT5G67180.3 49.112 169 78 3 231 399 97 257 6.79e-39 143
Msa1413020 AT5G67180.1 49.419 172 73 4 231 399 97 257 2.12e-38 144
Msa1413020 AT2G28550.3 50.000 164 74 3 231 394 154 309 5.07e-38 145
Msa1413020 AT2G41710.3 42.273 220 63 6 217 381 57 267 1.48e-35 138
Msa1413020 AT3G54320.2 57.377 122 48 2 304 422 1 121 1.87e-31 123
Msa1413020 AT2G28550.4 39.062 192 108 4 231 422 154 336 7.38e-31 122
Msa1413020 AT1G16060.2 71.591 88 25 0 304 391 1 88 8.97e-31 119
Msa1413020 AT1G16060.4 71.591 88 25 0 304 391 1 88 8.97e-31 119
Msa1413020 AT1G16060.6 71.591 88 25 0 304 391 1 88 8.97e-31 119
Msa1413020 AT1G16060.5 71.591 88 25 0 304 391 1 88 8.97e-31 119
Msa1413020 AT5G67180.4 47.552 143 67 3 231 373 97 231 1.18e-29 117
Msa1413020 AT2G28550.1 45.122 164 67 4 231 394 154 294 1.54e-29 121
Msa1413020 AT2G28550.2 47.619 126 52 4 231 353 154 268 4.34e-25 105
Msa1413020 AT2G28550.6 47.619 126 52 4 231 353 154 268 4.34e-25 105
Msa1413020 AT2G28550.5 45.802 131 55 2 264 394 14 128 2.18e-22 97.8
Msa1413020 AT2G39250.2 45.545 101 48 2 231 331 109 202 2.19e-22 96.7
Msa1413020 AT2G39250.1 45.545 101 48 2 231 331 109 202 1.05e-21 96.7
Msa1413020 AT5G60120.6 36.000 200 90 8 227 414 157 330 1.32e-18 89.0
Msa1413020 AT5G60120.1 35.500 200 91 7 227 414 157 330 1.68e-18 88.6
Msa1413020 AT5G60120.2 35.821 201 91 8 227 415 157 331 2.16e-18 88.6
Msa1413020 AT5G60120.4 38.415 164 72 6 227 387 157 294 4.02e-18 87.0
Msa1413020 AT5G60120.3 38.415 164 72 6 227 387 157 294 4.02e-18 87.0
Msa1413020 AT5G60120.5 38.415 164 72 6 227 387 157 294 4.02e-18 87.0
Msa1413020 AT3G54990.2 46.226 106 44 3 231 333 123 218 2.29e-17 82.4
Msa1413020 AT3G54990.1 45.631 103 49 2 231 333 123 218 1.15e-16 82.0
Msa1413020 AT1G16060.7 74.138 58 15 0 334 391 9 66 1.50e-16 78.6
Msa1413020 AT1G36060.1 45.000 80 43 1 312 391 125 203 6.27e-11 64.3

Find 136 sgRNAs with CRISPR-Local

Find 271 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCGGCTGAGTTCTATTTA+TGG 0.191348 tig0023989:-33364 Msa1413020:CDS
GGCTCTGAAGTTGATGATTC+TGG 0.212206 tig0023989:-37218 Msa1413020:CDS
ATTTGCGCCTCGGAACTTTA+TGG 0.216723 tig0023989:+33757 None:intergenic
GTTGGTGTTGGTGCTGGTGC+TGG 0.220856 tig0023989:+37288 None:intergenic
AAGAAGATTGTTGATACTTT+TGG 0.222886 tig0023989:-36996 Msa1413020:CDS
CACGGTAAATTGAAGTTCTT+TGG 0.247591 tig0023989:+36973 None:intergenic
ACCGCTGCTGCGGCAGCGGT+GGG 0.290599 tig0023989:-37104 Msa1413020:CDS
GTTGATGATTCTGGTTCAAT+AGG 0.295762 tig0023989:-37209 Msa1413020:CDS
CGCTGCTGCGGCAGCGGTGG+GGG 0.299820 tig0023989:-37102 Msa1413020:CDS
CAACACCACCAGGATCTTAC+TGG 0.305721 tig0023989:-37269 Msa1413020:CDS
TTTGGCTGCTCTAAAGTATT+GGG 0.306366 tig0023989:-34844 Msa1413020:CDS
GAAGATATGAAGCCCATCTA+TGG 0.317062 tig0023989:-36402 Msa1413020:CDS
GCGAGAATTGGCCGTGTTGC+CGG 0.321909 tig0023989:-33965 Msa1413020:CDS
TACAGCTGTTATCCCATAGA+TGG 0.324143 tig0023989:+36389 None:intergenic
TTCCAATTATGCAAAAGAAT+TGG 0.341907 tig0023989:-34280 Msa1413020:CDS
GACAACTTGTATACTCATAA+TGG 0.342173 tig0023989:-37720 Msa1413020:intron
CGTTTAGCTGCCCCGCCAAC+TGG 0.345030 tig0023989:+33674 None:intergenic
CACCGCTGCTGCGGCAGCGG+TGG 0.352088 tig0023989:-37105 Msa1413020:CDS
CTACAAATTCGAATAATAAC+TGG 0.352540 tig0023989:-37851 Msa1413020:CDS
GACTTGTATCTCGGAACATT+TGG 0.362363 tig0023989:-33935 Msa1413020:intron
TTGTTCTTGTTCTTCTGCTT+TGG 0.368361 tig0023989:+37463 None:intergenic
TCAAGTCAATAGGACTGATT+TGG 0.370990 tig0023989:+33341 None:intergenic
CTGATTCTAGAGAACGCTTC+AGG 0.377598 tig0023989:+33651 None:intergenic
TTGGTGTTGGTGCTGGTGCT+GGG 0.377768 tig0023989:+37289 None:intergenic
CCTAGAAAATCTTCTAGCTT+TGG 0.385172 tig0023989:+37392 None:intergenic
CCTTGTGACACCCCGGTAGA+TGG 0.387528 tig0023989:+34098 None:intergenic
CTCCACCGCTGCTGCGGCAG+CGG 0.387857 tig0023989:-37108 Msa1413020:CDS
GAGATACAAGTCTTTGTTCC+CGG 0.390452 tig0023989:+33946 None:intergenic
TTCCTGCAGGCATCACCAAC+AGG 0.398212 tig0023989:-33999 Msa1413020:intron
GTTGGTGCTGGTGCTGGGAG+TGG 0.414979 tig0023989:+37294 None:intergenic
GCTGCATGTGGGAATGAGTT+TGG 0.416652 tig0023989:-37173 Msa1413020:CDS
GGTAGGTGGCAAGCGAGAAT+TGG 0.417872 tig0023989:-33977 Msa1413020:CDS
TCTGGTTCAATAGGGAAATC+AGG 0.420422 tig0023989:-37200 Msa1413020:CDS
GGCCGCAAGATCCTATGATT+TGG 0.422462 tig0023989:-34862 Msa1413020:CDS
GGTGGTGTTGGTGTTGGTGC+TGG 0.423875 tig0023989:+37282 None:intergenic
CAGCAACAACATCAACTTCT+CGG 0.424028 tig0023989:-33549 Msa1413020:CDS
ATTTGGCTGCTCTAAAGTAT+TGG 0.427354 tig0023989:-34845 Msa1413020:CDS
TATGGGATAACAGCTGTAGA+AGG 0.428845 tig0023989:-36384 Msa1413020:CDS
CACCGCTGCCGCAGCAGCGG+TGG 0.431278 tig0023989:+37106 None:intergenic
AAACACTACTTCAAGTCAAT+AGG 0.436256 tig0023989:+33331 None:intergenic
GTTGTTGCTGCTGCTGCTGT+TGG 0.444214 tig0023989:+33562 None:intergenic
CCCCACCGCTGCCGCAGCAG+CGG 0.444418 tig0023989:+37103 None:intergenic
CGAGAATTGGCCGTGTTGCC+GGG 0.446110 tig0023989:-33964 Msa1413020:CDS
CTTTAGAGCAGCCAAATCAT+AGG 0.447422 tig0023989:+34851 None:intergenic
AGAACAATCAATCTGGTTTG+TGG 0.454287 tig0023989:-37447 Msa1413020:CDS
CCGCTGCTGCGGCAGCGGTG+GGG 0.462790 tig0023989:-37103 Msa1413020:CDS
CATTGGTTGCTGATGTTGAA+TGG 0.463694 tig0023989:+33526 None:intergenic
ATCATCATCACTACTTTGGT+GGG 0.463957 tig0023989:-37319 Msa1413020:CDS
TTGATGATTCTGGTTCAATA+GGG 0.466762 tig0023989:-37208 Msa1413020:CDS
CAAGAGGAGCTTCCATCTAC+CGG 0.470693 tig0023989:-34110 Msa1413020:CDS
AGAGCTCTCTTCCAGTTGGC+GGG 0.475251 tig0023989:-33685 Msa1413020:CDS
ATGTTTGCAGCACTTTGTTG+AGG 0.477979 tig0023989:+33587 None:intergenic
TTTCATGCTAGTACCAATGA+TGG 0.480177 tig0023989:-33434 Msa1413020:CDS
GCACCAACACCAACACCACC+AGG 0.481866 tig0023989:-37279 Msa1413020:CDS
AAGAACAAGAACAATCAATC+TGG 0.483167 tig0023989:-37454 Msa1413020:CDS
TGTTTGCAGCACTTTGTTGA+GGG 0.486036 tig0023989:+33588 None:intergenic
TATGATCATCATCACTACTT+TGG 0.486383 tig0023989:-37323 Msa1413020:CDS
AGAAAATCTTCTAGCTTTGG+TGG 0.488892 tig0023989:+37395 None:intergenic
ATGAGAGGAGTTAGTAGTAA+CGG 0.489643 tig0023989:+37767 None:intergenic
AAGGGAAGGTCAAGCTAGGA+AGG 0.489953 tig0023989:-36365 Msa1413020:CDS
TGATTCTGCAGCTCCATCAT+TGG 0.490937 tig0023989:+33421 None:intergenic
TGGATATGACAAAGAAGAAA+AGG 0.490955 tig0023989:-34883 Msa1413020:CDS
TTGTCACTGCATTTGCGCCT+CGG 0.493838 tig0023989:+33747 None:intergenic
CAATTTACCGTGGTGTCACT+AGG 0.494957 tig0023989:-36962 Msa1413020:intron
GCTGCAGAATCAGCAGCAAA+TGG 0.504223 tig0023989:-33410 Msa1413020:CDS
GATCCTGGTGGTGTTGGTGT+TGG 0.509940 tig0023989:+37276 None:intergenic
TTGGCTGCTCTAAAGTATTG+GGG 0.511428 tig0023989:-34843 Msa1413020:CDS
TGGTACTAGCATGAAAATGC+TGG 0.516205 tig0023989:+33441 None:intergenic
GATCATCATCACTACTTTGG+TGG 0.521100 tig0023989:-37320 Msa1413020:CDS
AGTGACTCTTACAGGGAAGT+TGG 0.524758 tig0023989:+34806 None:intergenic
AAATCTTCTAGCTTTGGTGG+TGG 0.525839 tig0023989:+37398 None:intergenic
TGCTTACTCCACCGCTGCTG+CGG 0.529248 tig0023989:-37114 Msa1413020:CDS
TTTATACTAGGGTGGGGAAA+CGG 0.529987 tig0023989:-37488 Msa1413020:intron
CTCAGCCGATGTTGGTGGCA+TGG 0.530259 tig0023989:+33376 None:intergenic
AACTGAAGAGGAAGCTGCAG+AGG 0.531137 tig0023989:-33795 Msa1413020:CDS
CTTGAAAACCAGTAAGATCC+TGG 0.531357 tig0023989:+37261 None:intergenic
TCGCTTGCCACCTACCCTGT+TGG 0.531381 tig0023989:+33984 None:intergenic
AAGAGCTCTCTTCCAGTTGG+CGG 0.536065 tig0023989:-33686 Msa1413020:CDS
CTAGCTTTGGTGGTGGAGTG+TGG 0.537501 tig0023989:+37405 None:intergenic
AATTCATTGCTTCATTGAGA+AGG 0.538177 tig0023989:-34230 Msa1413020:intron
AACAAACTGAGGCATTTCCA+TGG 0.538307 tig0023989:+37809 None:intergenic
CCATCTACCGGGGTGTCACA+AGG 0.539236 tig0023989:-34098 Msa1413020:intron
GAGTCATACGGAATACCATA+GGG 0.544015 tig0023989:+33494 None:intergenic
TAGCATGAAAATGCTGGAAG+AGG 0.547125 tig0023989:+33447 None:intergenic
CGAGTCATACGGAATACCAT+AGG 0.549420 tig0023989:+33493 None:intergenic
CCAATGGCTTCAATCCCCTA+TGG 0.549466 tig0023989:-33509 Msa1413020:CDS
TAGAACTCAGCCGATGTTGG+TGG 0.553420 tig0023989:+33371 None:intergenic
AAGAGGAGCTTCCATCTACC+GGG 0.554347 tig0023989:-34109 Msa1413020:CDS
AAGATATGAAGCCCATCTAT+GGG 0.555486 tig0023989:-36401 Msa1413020:CDS
CCATAGGGGATTGAAGCCAT+TGG 0.565801 tig0023989:+33509 None:intergenic
TGGCTGCTCTAAAGTATTGG+GGG 0.567440 tig0023989:-34842 Msa1413020:CDS
AAATAGAACTCAGCCGATGT+TGG 0.570966 tig0023989:+33368 None:intergenic
TATGAGTATACAAGTTGTCA+AGG 0.571623 tig0023989:+37724 None:intergenic
GCTAATGGAACTCTGTCGCT+CGG 0.572989 tig0023989:-37080 Msa1413020:CDS
GTAGAAGGGAAGGTCAAGCT+AGG 0.574052 tig0023989:-36369 Msa1413020:CDS
TAGTAGTAACGGAAGAAGCT+TGG 0.580648 tig0023989:+37778 None:intergenic
CATGATTACAGCAACTGAAG+AGG 0.581404 tig0023989:-33807 Msa1413020:intron
AAGAGTAGTGGCTTCTCAAG+AGG 0.582094 tig0023989:-34126 Msa1413020:CDS
CAATGCCATGCCACCAACAT+CGG 0.582869 tig0023989:-33381 Msa1413020:CDS
ACAGCTGTTATCCCATAGAT+GGG 0.584544 tig0023989:+36390 None:intergenic
AGGGAAGTTGGTAGTAGCAG+TGG 0.587800 tig0023989:+34818 None:intergenic
GGGAACAAAGACTTGTATCT+CGG 0.588471 tig0023989:-33944 Msa1413020:CDS
CTTTATGGCCGCAATGTCGT+AGG 0.589259 tig0023989:+33772 None:intergenic
AAATCAGGACAAGCTGCATG+TGG 0.591247 tig0023989:-37185 Msa1413020:CDS
GGCATCACCAACAGGGTAGG+TGG 0.595697 tig0023989:-33991 Msa1413020:CDS
TATTCCGTATGACTCGAATA+CGG 0.597263 tig0023989:-33486 Msa1413020:CDS
CAGTAAGATCCTGGTGGTGT+TGG 0.600392 tig0023989:+37270 None:intergenic
ATGGGATAACAGCTGTAGAA+GGG 0.605627 tig0023989:-36383 Msa1413020:CDS
AGGGAAGGTCAAGCTAGGAA+GGG 0.607036 tig0023989:-36364 Msa1413020:CDS
ACAAACTGAGGCATTTCCAT+GGG 0.608871 tig0023989:+37810 None:intergenic
ATGAAGAGCTCTCTTCCAGT+TGG 0.609750 tig0023989:-33689 Msa1413020:CDS
TGCAGAGGCCTACGACATTG+CGG 0.609893 tig0023989:-33780 Msa1413020:CDS
AGCCAAATCATAGGATCTTG+CGG 0.610099 tig0023989:+34860 None:intergenic
TCTTCTTGCAGGAAGAGTAG+TGG 0.611461 tig0023989:-34138 Msa1413020:intron
GATAACAGCTGTAGAAGGGA+AGG 0.621863 tig0023989:-36379 Msa1413020:CDS
CGGTGTTGCGCAGAGTTCCG+AGG 0.623641 tig0023989:-37060 Msa1413020:CDS
GAGCTCTCTTCCAGTTGGCG+GGG 0.623879 tig0023989:-33684 Msa1413020:CDS
CAAGCTAGGAAGGGTCGTCA+AGG 0.628154 tig0023989:-36355 Msa1413020:intron
CATTCAACATCAGCAACCAA+TGG 0.631785 tig0023989:-33525 Msa1413020:CDS
GCAGGCATCACCAACAGGGT+AGG 0.639876 tig0023989:-33994 Msa1413020:intron
GGGAAGTTGGTAGTAGCAGT+GGG 0.646966 tig0023989:+34819 None:intergenic
TCCTGCAGGCATCACCAACA+GGG 0.649115 tig0023989:-33998 Msa1413020:intron
AATCAGGACAAGCTGCATGT+GGG 0.650674 tig0023989:-37184 Msa1413020:CDS
GCAGAATCAGCAGCAAATGG+AGG 0.655444 tig0023989:-33407 Msa1413020:CDS
TAAGCCGTATTCGAGTCATA+CGG 0.655944 tig0023989:+33482 None:intergenic
AGTCTTTGTTCCCGGCAACA+CGG 0.656378 tig0023989:+33954 None:intergenic
TTACAAACCTTGTGACACCC+CGG 0.662260 tig0023989:+34091 None:intergenic
TCATCATCACTACTTTGGTG+GGG 0.667284 tig0023989:-37318 Msa1413020:CDS
CAAAGAACTTCAATTTACCG+TGG 0.676500 tig0023989:-36972 Msa1413020:CDS
ATTGCGGCCATAAAGTTCCG+AGG 0.680301 tig0023989:-33764 Msa1413020:CDS
TGGTCATATTGAACAAACTG+AGG 0.687500 tig0023989:+37798 None:intergenic
TATTATACCTAGTGACACCA+CGG 0.692652 tig0023989:+36955 None:intergenic
TGAGATGAACAGATATGACG+TGG 0.711855 tig0023989:-33720 Msa1413020:CDS
AGAGGAGCTTCCATCTACCG+GGG 0.715037 tig0023989:-34108 Msa1413020:CDS
AGTCATACGGAATACCATAG+GGG 0.731791 tig0023989:+33495 None:intergenic
GAAAACCAGTAAGATCCTGG+TGG 0.756363 tig0023989:+37264 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTATGATTTTAATTTG+GGG - tig0023989:34765-34784 Msa1413020:intron 10.0%
!!! AACATACTTTTATTTTATTT+TGG - tig0023989:36014-36033 Msa1413020:intron 10.0%
!!! TTAGATTATAAAGTAATTTT+TGG - tig0023989:36050-36069 Msa1413020:intron 10.0%
!!! AATTTTATTTCTTAATCTAA+CGG + tig0023989:36717-36736 None:intergenic 10.0%
!! AAAAACAATAACAATATATA+AGG + tig0023989:36896-36915 None:intergenic 10.0%
!!! GTATTTTTTACAATTTTAAT+AGG + tig0023989:37023-37042 None:intergenic 10.0%
!! AAAAAAACAAATATTCATGT+AGG + tig0023989:33645-33664 None:intergenic 15.0%
!!! TCATTGTTTTTTTTTTTGAA+AGG - tig0023989:34336-34355 Msa1413020:intron 15.0%
!!! TAATTATTTTTTGTATCCAA+AGG - tig0023989:34422-34441 Msa1413020:intron 15.0%
!!! ATAATATATGCTTTTTCATT+TGG - tig0023989:35113-35132 Msa1413020:intron 15.0%
!! TGTTACTAGTAAAATTAAAA+GGG - tig0023989:35367-35386 Msa1413020:intron 15.0%
!!! TTTATATTGTTTTAGAGATA+TGG - tig0023989:36267-36286 Msa1413020:intron 15.0%
!! GTAAATTAATCTTAATTCAA+TGG + tig0023989:36489-36508 None:intergenic 15.0%
!! TAATTTACAATTCATATTCA+TGG - tig0023989:36501-36520 Msa1413020:intron 15.0%
!!! AAATCCATTTTGAAATTATA+AGG - tig0023989:36791-36810 Msa1413020:intron 15.0%
!!! TTCTTTGTTTAACATTATTT+TGG - tig0023989:37191-37210 Msa1413020:CDS 15.0%
!! CAAAGACATTTGATATATTA+GGG + tig0023989:33551-33570 None:intergenic 20.0%
!! ACAAAGACATTTGATATATT+AGG + tig0023989:33552-33571 None:intergenic 20.0%
!!! TTGTTTTGACTATTTATACT+AGG - tig0023989:33713-33732 Msa1413020:CDS 20.0%
!!! TGTTTTGACTATTTATACTA+GGG - tig0023989:33714-33733 Msa1413020:CDS 20.0%
!! ACAAATAAAAAAAGATCCTT+TGG + tig0023989:34441-34460 None:intergenic 20.0%
!!! ATTTATTGACTATGTTATGT+TGG - tig0023989:34620-34639 Msa1413020:intron 20.0%
!!! GTGTTACTAGTAAAATTAAA+AGG - tig0023989:35366-35385 Msa1413020:intron 20.0%
!!! AAAATTTGTCTATTTCTCAT+TGG + tig0023989:35704-35723 None:intergenic 20.0%
!! TCATGTTAACAAAATACTAT+TGG + tig0023989:35765-35784 None:intergenic 20.0%
!! AGATAGAGAGATAAAATTAA+AGG - tig0023989:35956-35975 Msa1413020:intron 20.0%
!! GATAGAGAGATAAAATTAAA+GGG - tig0023989:35957-35976 Msa1413020:intron 20.0%
!!! TATGATTTTTCTTTGTACAT+TGG + tig0023989:36447-36466 None:intergenic 20.0%
!! AATGTACAAAGAAAAATCAT+AGG - tig0023989:36446-36465 Msa1413020:intron 20.0%
!!! ATTTTCTACAATCAACTTTA+CGG + tig0023989:36775-36794 None:intergenic 20.0%
!! CAATCCTTATAATTTCAAAA+TGG + tig0023989:36798-36817 None:intergenic 20.0%
!! TAATCTCAATTCTTACTTAA+TGG + tig0023989:36840-36859 None:intergenic 20.0%
!! GTAAGAATTGAGATTATATA+TGG - tig0023989:36844-36863 Msa1413020:intron 20.0%
!!! TGTTTTTTTTCCTATATCTT+AGG - tig0023989:36909-36928 Msa1413020:intron 20.0%
!! AAAAAATACTTATCATGATC+AGG - tig0023989:37034-37053 Msa1413020:CDS 20.0%
!! ACAATACTTCTAATACTTTA+AGG + tig0023989:37316-37335 None:intergenic 20.0%
!!! AGTATTGTCTTAAATTTTGT+TGG - tig0023989:37328-37347 Msa1413020:CDS 20.0%
! CTACAAATTCGAATAATAAC+TGG - tig0023989:33362-33381 Msa1413020:CDS 25.0%
! AAACAAATATTCATGTAGGT+CGG + tig0023989:33641-33660 None:intergenic 25.0%
!! AAGAAGATTGTTGATACTTT+TGG - tig0023989:34217-34236 Msa1413020:intron 25.0%
! TTGTTTGATTGAAAATCCAA+AGG - tig0023989:34457-34476 Msa1413020:intron 25.0%
! TCAAGTTCATAGATTTGATT+TGG - tig0023989:34660-34679 Msa1413020:intron 25.0%
! AATTAAACAGTGTTTGATTC+TGG - tig0023989:34947-34966 Msa1413020:intron 25.0%
! ATTAAACAGTGTTTGATTCT+GGG - tig0023989:34948-34967 Msa1413020:intron 25.0%
!!! ATTAATGGTTTTAGAGTTGT+AGG - tig0023989:35003-35022 Msa1413020:intron 25.0%
!!! TAGTACTATGATTGATTTGA+TGG - tig0023989:35043-35062 Msa1413020:intron 25.0%
! CACAGTTATGAATATTAGTA+CGG + tig0023989:35335-35354 None:intergenic 25.0%
!! TACTTTAACTTACACTTATG+AGG - tig0023989:35495-35514 Msa1413020:intron 25.0%
!!! GAACATAGAGAAAACTTTTT+TGG + tig0023989:35672-35691 None:intergenic 25.0%
! CAGATATGTTTATTTGACTA+TGG - tig0023989:35734-35753 Msa1413020:intron 25.0%
! AAATTTGACTATATGCCATA+AGG - tig0023989:36078-36097 Msa1413020:intron 25.0%
! AATTTGACTATATGCCATAA+GGG - tig0023989:36079-36098 Msa1413020:intron 25.0%
! AATATCAAATATCACCCTTA+TGG + tig0023989:36096-36115 None:intergenic 25.0%
! TGTAACATTGTAAATTGTCA+TGG - tig0023989:36294-36313 Msa1413020:intron 25.0%
! TGAAAGAAATATGCTTTACT+TGG - tig0023989:36586-36605 Msa1413020:intron 25.0%
!! TCAATTCTTACTTAATGGAT+AGG + tig0023989:36835-36854 None:intergenic 25.0%
! TATATAAGGTAAGTCTTGAA+AGG + tig0023989:36882-36901 None:intergenic 25.0%
! TAATTGGAAACCTAAGATAT+AGG + tig0023989:36922-36941 None:intergenic 25.0%
! TTCCAATTATGCAAAAGAAT+TGG - tig0023989:36933-36952 Msa1413020:intron 25.0%
!! TTCCAATTCTTTTGCATAAT+TGG + tig0023989:36938-36957 None:intergenic 25.0%
!! GTCTTAAATTTTGTTGGAAA+AGG - tig0023989:37334-37353 Msa1413020:CDS 25.0%
TATGAGTATACAAGTTGTCA+AGG + tig0023989:33492-33511 None:intergenic 30.0%
GACAACTTGTATACTCATAA+TGG - tig0023989:33493-33512 Msa1413020:CDS 30.0%
TTTGACTATTTATACTAGGG+TGG - tig0023989:33717-33736 Msa1413020:CDS 30.0%
TTGACTATTTATACTAGGGT+GGG - tig0023989:33718-33737 Msa1413020:CDS 30.0%
AAGAACAAGAACAATCAATC+TGG - tig0023989:33759-33778 Msa1413020:CDS 30.0%
TATGATCATCATCACTACTT+TGG - tig0023989:33890-33909 Msa1413020:intron 30.0%
!! TTGATGATTCTGGTTCAATA+GGG - tig0023989:34005-34024 Msa1413020:intron 30.0%
!! GATCTTTTGTTGTCACTATT+TGG - tig0023989:34479-34498 Msa1413020:intron 30.0%
!! TAGCTATTGATTTACTCAGT+TGG - tig0023989:34531-34550 Msa1413020:intron 30.0%
!!! TTTTAATTTGGGGATTTTGC+AGG - tig0023989:34775-34794 Msa1413020:intron 30.0%
ATGCATTGAAGTTGCATTAA+TGG - tig0023989:34988-35007 Msa1413020:intron 30.0%
ATAGTACTAATAAGTAACCC+AGG + tig0023989:35032-35051 None:intergenic 30.0%
!! CTATGATTGATTTGATGGTA+TGG - tig0023989:35048-35067 Msa1413020:intron 30.0%
TCTGTTCAGATAGTTATGAA+GGG - tig0023989:35245-35264 Msa1413020:intron 30.0%
TTAGTACGGAATTAATCACT+TGG + tig0023989:35321-35340 None:intergenic 30.0%
ACAAATTTCAATACAGTGAG+TGG + tig0023989:35476-35495 None:intergenic 30.0%
ATGAGTCCTTGTAGTAAAAA+AGG - tig0023989:35861-35880 Msa1413020:intron 30.0%
TGAGTCCTTGTAGTAAAAAA+GGG - tig0023989:35862-35881 Msa1413020:intron 30.0%
GATAGAAAACTACTGTACAT+TGG + tig0023989:35918-35937 None:intergenic 30.0%
AGGGTGATATTTGATATTCT+TGG - tig0023989:36098-36117 Msa1413020:intron 30.0%
TGGATATGACAAAGAAGAAA+AGG - tig0023989:36330-36349 Msa1413020:intron 30.0%
AAACACATTATGTTCACACA+TGG + tig0023989:36562-36581 None:intergenic 30.0%
ATGTGTGAACATAATGTGTT+TGG - tig0023989:36561-36580 Msa1413020:intron 30.0%
ACTTGGTACACAAAATATTG+AGG - tig0023989:36603-36622 Msa1413020:intron 30.0%
TACACAAAATATTGAGGCTA+AGG - tig0023989:36609-36628 Msa1413020:intron 30.0%
TGGTATCTTAATAAGAGAAC+TGG - tig0023989:36646-36665 Msa1413020:intron 30.0%
AATTCATTGCTTCATTGAGA+AGG - tig0023989:36983-37002 Msa1413020:CDS 30.0%
CATGATCAGGTACTAAAATT+TGG - tig0023989:37047-37066 Msa1413020:CDS 30.0%
AGTGTACAGATTGTATTACA+CGG - tig0023989:37168-37187 Msa1413020:CDS 30.0%
! ATCATTTTCACTTTCACCCA+TGG - tig0023989:33387-33406 Msa1413020:CDS 35.0%
TGGTCATATTGAACAAACTG+AGG + tig0023989:33418-33437 None:intergenic 35.0%
ATGAGAGGAGTTAGTAGTAA+CGG + tig0023989:33449-33468 None:intergenic 35.0%
!! GTGATGATGATTTTGATGAG+AGG + tig0023989:33464-33483 None:intergenic 35.0%
TACTTAACGCCGTTTAACAA+CGG - tig0023989:33594-33613 Msa1413020:CDS 35.0%
!! CTTTTCTAGCCGTTGTTAAA+CGG + tig0023989:33606-33625 None:intergenic 35.0%
TGACTATTTATACTAGGGTG+GGG - tig0023989:33719-33738 Msa1413020:CDS 35.0%
TTGTTCTTGTTCTTCTGCTT+TGG + tig0023989:33753-33772 None:intergenic 35.0%
! AGAACAATCAATCTGGTTTG+TGG - tig0023989:33766-33785 Msa1413020:CDS 35.0%
!! AGAAAATCTTCTAGCTTTGG+TGG + tig0023989:33821-33840 None:intergenic 35.0%
!! CCTAGAAAATCTTCTAGCTT+TGG + tig0023989:33824-33843 None:intergenic 35.0%
! CCAAAGCTAGAAGATTTTCT+AGG - tig0023989:33821-33840 Msa1413020:intron 35.0%
ATCATCATCACTACTTTGGT+GGG - tig0023989:33894-33913 Msa1413020:intron 35.0%
!!! GCTTTTTCAAACAACAACTC+TGG - tig0023989:33974-33993 Msa1413020:CDS 35.0%
!! GTTGATGATTCTGGTTCAAT+AGG - tig0023989:34004-34023 Msa1413020:intron 35.0%
! AGCAACAATAGCATTTTCCT+CGG + tig0023989:34173-34192 None:intergenic 35.0%
CACGGTAAATTGAAGTTCTT+TGG + tig0023989:34243-34262 None:intergenic 35.0%
CAAAGAACTTCAATTTACCG+TGG - tig0023989:34241-34260 Msa1413020:CDS 35.0%
TATTATACCTAGTGACACCA+CGG + tig0023989:34261-34280 None:intergenic 35.0%
AGTGACAACAAAAGATCCTT+TGG + tig0023989:34476-34495 None:intergenic 35.0%
AAGATATGAAGCCCATCTAT+GGG - tig0023989:34812-34831 Msa1413020:intron 35.0%
GTCTGTTCAGATAGTTATGA+AGG - tig0023989:35244-35263 Msa1413020:intron 35.0%
AACGTTCACTGTTACATGAA+AGG - tig0023989:35524-35543 Msa1413020:intron 35.0%
GAGTCCTTGTAGTAAAAAAG+GGG - tig0023989:35863-35882 Msa1413020:intron 35.0%
AGTCCTTGTAGTAAAAAAGG+GGG - tig0023989:35864-35883 Msa1413020:intron 35.0%
!! ATCTTTATAGCTTGCTACGT+AGG + tig0023989:35940-35959 None:intergenic 35.0%
AGACTGTAGATGAGTCAAAA+AGG + tig0023989:36168-36187 None:intergenic 35.0%
TGACTCATCTACAGTCTATT+TGG - tig0023989:36171-36190 Msa1413020:intron 35.0%
GACTCATCTACAGTCTATTT+GGG - tig0023989:36172-36191 Msa1413020:intron 35.0%
CATTGTAAATTGTCATGGCA+TGG - tig0023989:36299-36318 Msa1413020:intron 35.0%
!! ATTTGGCTGCTCTAAAGTAT+TGG - tig0023989:36368-36387 Msa1413020:CDS 35.0%
!! TTTGGCTGCTCTAAAGTATT+GGG - tig0023989:36369-36388 Msa1413020:CDS 35.0%
ACAAAGAAGTGACTCTTACA+GGG + tig0023989:36417-36436 None:intergenic 35.0%
AACAAAGAAGTGACTCTTAC+AGG + tig0023989:36418-36437 None:intergenic 35.0%
ACAAAATATTGAGGCTAAGG+TGG - tig0023989:36612-36631 Msa1413020:intron 35.0%
! TCTTACTTAATGGATAGGCA+TGG + tig0023989:36830-36849 None:intergenic 35.0%
ATTTGGTTATCTCTTCTTGC+AGG - tig0023989:37064-37083 Msa1413020:CDS 35.0%
!!! AACATTATTTTGGTTCCTGC+AGG - tig0023989:37201-37220 Msa1413020:CDS 35.0%
TATTCCGTATGACTCGAATA+CGG - tig0023989:37727-37746 Msa1413020:CDS 35.0%
TTTCATGCTAGTACCAATGA+TGG - tig0023989:37779-37798 Msa1413020:CDS 35.0%
! ACAAACTGAGGCATTTCCAT+GGG + tig0023989:33406-33425 None:intergenic 40.0%
! AACAAACTGAGGCATTTCCA+TGG + tig0023989:33407-33426 None:intergenic 40.0%
! TAGTAGTAACGGAAGAAGCT+TGG + tig0023989:33438-33457 None:intergenic 40.0%
TTTATACTAGGGTGGGGAAA+CGG - tig0023989:33725-33744 Msa1413020:CDS 40.0%
!! AAATCTTCTAGCTTTGGTGG+TGG + tig0023989:33818-33837 None:intergenic 40.0%
GATCATCATCACTACTTTGG+TGG - tig0023989:33893-33912 Msa1413020:intron 40.0%
TCATCATCACTACTTTGGTG+GGG - tig0023989:33895-33914 Msa1413020:intron 40.0%
CTTGAAAACCAGTAAGATCC+TGG + tig0023989:33955-33974 None:intergenic 40.0%
! TCTGGTTCAATAGGGAAATC+AGG - tig0023989:34013-34032 Msa1413020:intron 40.0%
GAAGATATGAAGCCCATCTA+TGG - tig0023989:34811-34830 Msa1413020:intron 40.0%
ACAGCTGTTATCCCATAGAT+GGG + tig0023989:34826-34845 None:intergenic 40.0%
TACAGCTGTTATCCCATAGA+TGG + tig0023989:34827-34846 None:intergenic 40.0%
TATGGGATAACAGCTGTAGA+AGG - tig0023989:34829-34848 Msa1413020:CDS 40.0%
ATGGGATAACAGCTGTAGAA+GGG - tig0023989:34830-34849 Msa1413020:CDS 40.0%
!!! TTTTAGAGTTGTAGGACACC+TGG - tig0023989:35011-35030 Msa1413020:intron 40.0%
! TTTAGAGTTGTAGGACACCT+GGG - tig0023989:35012-35031 Msa1413020:intron 40.0%
ATTCACATGGTGCAGCAAAA+TGG + tig0023989:35205-35224 None:intergenic 40.0%
! TTTTGCTGCACCATGTGAAT+TGG - tig0023989:35205-35224 Msa1413020:intron 40.0%
TTTGCTGCACCATGTGAATT+GGG - tig0023989:35206-35225 Msa1413020:intron 40.0%
CTGAACAGACAAGAAAGCAA+AGG + tig0023989:35234-35253 None:intergenic 40.0%
AATACAGTGAGTGGTGTGAT+AGG + tig0023989:35467-35486 None:intergenic 40.0%
!!! CAACCCCCTTTTTTACTACA+AGG + tig0023989:35870-35889 None:intergenic 40.0%
!! TTCTTGGCTTTTCCTTTGCA+TGG - tig0023989:36114-36133 Msa1413020:intron 40.0%
! ATTGTCATGGCATGGTTTGT+AGG - tig0023989:36307-36326 Msa1413020:intron 40.0%
AGCCAAATCATAGGATCTTG+CGG + tig0023989:36356-36375 None:intergenic 40.0%
!! CTTTAGAGCAGCCAAATCAT+AGG + tig0023989:36365-36384 None:intergenic 40.0%
!! TTGGCTGCTCTAAAGTATTG+GGG - tig0023989:36370-36389 Msa1413020:CDS 40.0%
CTAAGGTGGCAATGTGAATT+TGG - tig0023989:36626-36645 Msa1413020:intron 40.0%
ATCAACTTTACGGAGCAGTT+TGG + tig0023989:36765-36784 None:intergenic 40.0%
GAGATACAAGTCTTTGTTCC+CGG + tig0023989:37270-37289 None:intergenic 40.0%
GGGAACAAAGACTTGTATCT+CGG - tig0023989:37269-37288 Msa1413020:CDS 40.0%
GACTTGTATCTCGGAACATT+TGG - tig0023989:37278-37297 Msa1413020:CDS 40.0%
CATGATTACAGCAACTGAAG+AGG - tig0023989:37406-37425 Msa1413020:CDS 40.0%
TGAGATGAACAGATATGACG+TGG - tig0023989:37493-37512 Msa1413020:intron 40.0%
TGTTTGCAGCACTTTGTTGA+GGG + tig0023989:37628-37647 None:intergenic 40.0%
ATGTTTGCAGCACTTTGTTG+AGG + tig0023989:37629-37648 None:intergenic 40.0%
CAGCAACAACATCAACTTCT+CGG - tig0023989:37664-37683 Msa1413020:intron 40.0%
! CATTGGTTGCTGATGTTGAA+TGG + tig0023989:37690-37709 None:intergenic 40.0%
CATTCAACATCAGCAACCAA+TGG - tig0023989:37688-37707 Msa1413020:intron 40.0%
AGTCATACGGAATACCATAG+GGG + tig0023989:37721-37740 None:intergenic 40.0%
GAGTCATACGGAATACCATA+GGG + tig0023989:37722-37741 None:intergenic 40.0%
TAAGCCGTATTCGAGTCATA+CGG + tig0023989:37734-37753 None:intergenic 40.0%
TAGCATGAAAATGCTGGAAG+AGG + tig0023989:37769-37788 None:intergenic 40.0%
TGGTACTAGCATGAAAATGC+TGG + tig0023989:37775-37794 None:intergenic 40.0%
AAATAGAACTCAGCCGATGT+TGG + tig0023989:37848-37867 None:intergenic 40.0%
! CATCGGCTGAGTTCTATTTA+TGG - tig0023989:37849-37868 Msa1413020:CDS 40.0%
GAAAACCAGTAAGATCCTGG+TGG + tig0023989:33952-33971 None:intergenic 45.0%
!! GGCTCTGAAGTTGATGATTC+TGG - tig0023989:33995-34014 Msa1413020:intron 45.0%
AAATCAGGACAAGCTGCATG+TGG - tig0023989:34028-34047 Msa1413020:intron 45.0%
AATCAGGACAAGCTGCATGT+GGG - tig0023989:34029-34048 Msa1413020:intron 45.0%
!! CAATTTACCGTGGTGTCACT+AGG - tig0023989:34251-34270 Msa1413020:CDS 45.0%
! TTTTGCAGGCATAGATGGAC+AGG - tig0023989:34789-34808 Msa1413020:intron 45.0%
GATAACAGCTGTAGAAGGGA+AGG - tig0023989:34834-34853 Msa1413020:CDS 45.0%
CCTCTCTCAGAACAAACTCA+CGG - tig0023989:35560-35579 Msa1413020:intron 45.0%
CTCTCTCAGAACAAACTCAC+GGG - tig0023989:35561-35580 Msa1413020:intron 45.0%
GTAGCGCATGCAAATGTGAT+TGG - tig0023989:35989-36008 Msa1413020:intron 45.0%
AAAAGCCAGCAATGCACCAA+GGG + tig0023989:36145-36164 None:intergenic 45.0%
!! TGGCTGCTCTAAAGTATTGG+GGG - tig0023989:36371-36390 Msa1413020:CDS 45.0%
AGTGACTCTTACAGGGAAGT+TGG + tig0023989:36410-36429 None:intergenic 45.0%
TCTTCTTGCAGGAAGAGTAG+TGG - tig0023989:37075-37094 Msa1413020:CDS 45.0%
AAGAGTAGTGGCTTCTCAAG+AGG - tig0023989:37087-37106 Msa1413020:CDS 45.0%
TTACAAACCTTGTGACACCC+CGG + tig0023989:37125-37144 None:intergenic 45.0%
ATTTGCGCCTCGGAACTTTA+TGG + tig0023989:37459-37478 None:intergenic 45.0%
ATGAAGAGCTCTCTTCCAGT+TGG - tig0023989:37524-37543 Msa1413020:intron 45.0%
! CTGATTCTAGAGAACGCTTC+AGG + tig0023989:37565-37584 None:intergenic 45.0%
CGAGTCATACGGAATACCAT+AGG + tig0023989:37723-37742 None:intergenic 45.0%
TGATTCTGCAGCTCCATCAT+TGG + tig0023989:37795-37814 None:intergenic 45.0%
!!! TTTTATTTATGATTTTAATT+TGG - tig0023989:34763-34782 Msa1413020:intron 5.0%
!!! TTTATTTATGATTTTAATTT+GGG - tig0023989:34764-34783 Msa1413020:intron 5.0%
!!! TTTTCTATAATATTTTTTTA+TGG - tig0023989:34899-34918 Msa1413020:intron 5.0%
! CAGTAAGATCCTGGTGGTGT+TGG + tig0023989:33946-33965 None:intergenic 50.0%
CAACACCACCAGGATCTTAC+TGG - tig0023989:33944-33963 Msa1413020:CDS 50.0%
GCTGCATGTGGGAATGAGTT+TGG - tig0023989:34040-34059 Msa1413020:intron 50.0%
GCTAATGGAACTCTGTCGCT+CGG - tig0023989:34133-34152 Msa1413020:intron 50.0%
! GGGGATTTTGCAGGCATAGA+TGG - tig0023989:34784-34803 Msa1413020:intron 50.0%
GTAGAAGGGAAGGTCAAGCT+AGG - tig0023989:34844-34863 Msa1413020:CDS 50.0%
AAGGGAAGGTCAAGCTAGGA+AGG - tig0023989:34848-34867 Msa1413020:CDS 50.0%
AGGGAAGGTCAAGCTAGGAA+GGG - tig0023989:34849-34868 Msa1413020:CDS 50.0%
CACATGGTGCAGCAAAATGG+CGG + tig0023989:35202-35221 None:intergenic 50.0%
!! GCAAAGGCACCCAATTCACA+TGG + tig0023989:35218-35237 None:intergenic 50.0%
GAGTGGTGTGATAGGTGATG+TGG + tig0023989:35459-35478 None:intergenic 50.0%
!! CCGTGAGTTTGTTCTGAGAG+AGG + tig0023989:35563-35582 None:intergenic 50.0%
GAAAAGCCAGCAATGCACCA+AGG + tig0023989:36146-36165 None:intergenic 50.0%
! GTCATGGCATGGTTTGTAGG+TGG - tig0023989:36310-36329 Msa1413020:intron 50.0%
GGCCGCAAGATCCTATGATT+TGG - tig0023989:36351-36370 Msa1413020:intron 50.0%
!! GGGAAGTTGGTAGTAGCAGT+GGG + tig0023989:36397-36416 None:intergenic 50.0%
!! AGGGAAGTTGGTAGTAGCAG+TGG + tig0023989:36398-36417 None:intergenic 50.0%
CAAGAGGAGCTTCCATCTAC+CGG - tig0023989:37103-37122 Msa1413020:CDS 50.0%
AAGAGGAGCTTCCATCTACC+GGG - tig0023989:37104-37123 Msa1413020:CDS 50.0%
AGTCTTTGTTCCCGGCAACA+CGG + tig0023989:37262-37281 None:intergenic 50.0%
AACTGAAGAGGAAGCTGCAG+AGG - tig0023989:37418-37437 Msa1413020:CDS 50.0%
CTTTATGGCCGCAATGTCGT+AGG + tig0023989:37444-37463 None:intergenic 50.0%
ATTGCGGCCATAAAGTTCCG+AGG - tig0023989:37449-37468 Msa1413020:CDS 50.0%
TTGTCACTGCATTTGCGCCT+CGG + tig0023989:37469-37488 None:intergenic 50.0%
AAGAGCTCTCTTCCAGTTGG+CGG - tig0023989:37527-37546 Msa1413020:intron 50.0%
CCATAGGGGATTGAAGCCAT+TGG + tig0023989:37707-37726 None:intergenic 50.0%
CCAATGGCTTCAATCCCCTA+TGG - tig0023989:37704-37723 Msa1413020:intron 50.0%
GCTGCAGAATCAGCAGCAAA+TGG - tig0023989:37803-37822 Msa1413020:CDS 50.0%
GCAGAATCAGCAGCAAATGG+AGG - tig0023989:37806-37825 Msa1413020:CDS 50.0%
CAATGCCATGCCACCAACAT+CGG - tig0023989:37832-37851 Msa1413020:CDS 50.0%
TAGAACTCAGCCGATGTTGG+TGG + tig0023989:37845-37864 None:intergenic 50.0%
!! CTAGCTTTGGTGGTGGAGTG+TGG + tig0023989:33811-33830 None:intergenic 55.0%
!! TTGGTGTTGGTGCTGGTGCT+GGG + tig0023989:33927-33946 None:intergenic 55.0%
!! GATCCTGGTGGTGTTGGTGT+TGG + tig0023989:33940-33959 None:intergenic 55.0%
CAAGCTAGGAAGGGTCGTCA+AGG - tig0023989:34858-34877 Msa1413020:CDS 55.0%
AGAGGAGCTTCCATCTACCG+GGG - tig0023989:37105-37124 Msa1413020:CDS 55.0%
TTCCTGCAGGCATCACCAAC+AGG - tig0023989:37214-37233 Msa1413020:CDS 55.0%
TCCTGCAGGCATCACCAACA+GGG - tig0023989:37215-37234 Msa1413020:CDS 55.0%
GGTAGGTGGCAAGCGAGAAT+TGG - tig0023989:37236-37255 Msa1413020:CDS 55.0%
TGCAGAGGCCTACGACATTG+CGG - tig0023989:37433-37452 Msa1413020:CDS 55.0%
AGAGCTCTCTTCCAGTTGGC+GGG - tig0023989:37528-37547 Msa1413020:intron 55.0%
GTTGTTGCTGCTGCTGCTGT+TGG + tig0023989:37654-37673 None:intergenic 55.0%
!! GTTGGTGTTGGTGCTGGTGC+TGG + tig0023989:33928-33947 None:intergenic 60.0%
!! GGTGGTGTTGGTGTTGGTGC+TGG + tig0023989:33934-33953 None:intergenic 60.0%
GCACCAACACCAACACCACC+AGG - tig0023989:33934-33953 Msa1413020:intron 60.0%
TGCTTACTCCACCGCTGCTG+CGG - tig0023989:34099-34118 Msa1413020:CDS 60.0%
CCAAGGGACGGTCCATGCAA+AGG + tig0023989:36129-36148 None:intergenic 60.0%
CCTTTGCATGGACCGTCCCT+TGG - tig0023989:36126-36145 Msa1413020:intron 60.0%
! ACCGTCCCTTGGTGCATTGC+TGG - tig0023989:36137-36156 Msa1413020:intron 60.0%
GCCAGCAATGCACCAAGGGA+CGG + tig0023989:36141-36160 None:intergenic 60.0%
CCTTGTGACACCCCGGTAGA+TGG + tig0023989:37118-37137 None:intergenic 60.0%
! CCATCTACCGGGGTGTCACA+AGG - tig0023989:37115-37134 Msa1413020:CDS 60.0%
!! ACCCTGTTGGTGATGCCTGC+AGG + tig0023989:37219-37238 None:intergenic 60.0%
GCAGGCATCACCAACAGGGT+AGG - tig0023989:37219-37238 Msa1413020:CDS 60.0%
GGCATCACCAACAGGGTAGG+TGG - tig0023989:37222-37241 Msa1413020:CDS 60.0%
TCGCTTGCCACCTACCCTGT+TGG + tig0023989:37232-37251 None:intergenic 60.0%
GCGAGAATTGGCCGTGTTGC+CGG - tig0023989:37248-37267 Msa1413020:CDS 60.0%
CGAGAATTGGCCGTGTTGCC+GGG - tig0023989:37249-37268 Msa1413020:CDS 60.0%
GAGCTCTCTTCCAGTTGGCG+GGG - tig0023989:37529-37548 Msa1413020:intron 60.0%
!! CTCAGCCGATGTTGGTGGCA+TGG + tig0023989:37840-37859 None:intergenic 60.0%
!! GTTGGTGCTGGTGCTGGGAG+TGG + tig0023989:33922-33941 None:intergenic 65.0%
!! CGGTGTTGCGCAGAGTTCCG+AGG - tig0023989:34153-34172 Msa1413020:intron 65.0%
CGTTTAGCTGCCCCGCCAAC+TGG + tig0023989:37542-37561 None:intergenic 65.0%
CTCCACCGCTGCTGCGGCAG+CGG - tig0023989:34105-34124 Msa1413020:CDS 75.0%
ACCGCTGCTGCGGCAGCGGT+GGG - tig0023989:34109-34128 Msa1413020:CDS 75.0%
GCGGCAGCGGTGGGGGCTAA+TGG - tig0023989:34118-34137 Msa1413020:CDS 75.0%
! CACCGCTGCCGCAGCAGCGG+TGG + tig0023989:34110-34129 None:intergenic 80.0%
! CACCGCTGCTGCGGCAGCGG+TGG - tig0023989:34108-34127 Msa1413020:CDS 80.0%
! CCCCACCGCTGCCGCAGCAG+CGG + tig0023989:34113-34132 None:intergenic 80.0%
! CCGCTGCTGCGGCAGCGGTG+GGG - tig0023989:34110-34129 Msa1413020:CDS 80.0%
! CGCTGCTGCGGCAGCGGTGG+GGG - tig0023989:34111-34130 Msa1413020:CDS 80.0%
Chromosome Type Strat End Strand Name
tig0023989 gene 33346 37889 33346 ID=Msa1413020;Name=Msa1413020
tig0023989 mRNA 33346 37889 33346 ID=Msa1413020-mRNA-1;Parent=Msa1413020;Name=Msa1413020-mRNA-1;_AED=0.08;_eAED=0.08;_QI=0|0.14|0|1|1|1|8|0|517
tig0023989 exon 37721 37889 37721 ID=Msa1413020-mRNA-1:exon:20949;Parent=Msa1413020-mRNA-1
tig0023989 exon 36963 37500 36963 ID=Msa1413020-mRNA-1:exon:20948;Parent=Msa1413020-mRNA-1
tig0023989 exon 36356 36438 36356 ID=Msa1413020-mRNA-1:exon:20947;Parent=Msa1413020-mRNA-1
tig0023989 exon 34818 34906 34818 ID=Msa1413020-mRNA-1:exon:20946;Parent=Msa1413020-mRNA-1
tig0023989 exon 34231 34304 34231 ID=Msa1413020-mRNA-1:exon:20945;Parent=Msa1413020-mRNA-1
tig0023989 exon 34099 34149 34099 ID=Msa1413020-mRNA-1:exon:20944;Parent=Msa1413020-mRNA-1
tig0023989 exon 33936 34012 33936 ID=Msa1413020-mRNA-1:exon:20943;Parent=Msa1413020-mRNA-1
tig0023989 exon 33346 33818 33346 ID=Msa1413020-mRNA-1:exon:20942;Parent=Msa1413020-mRNA-1
tig0023989 CDS 37721 37889 37721 ID=Msa1413020-mRNA-1:cds;Parent=Msa1413020-mRNA-1
tig0023989 CDS 36963 37500 36963 ID=Msa1413020-mRNA-1:cds;Parent=Msa1413020-mRNA-1
tig0023989 CDS 36356 36438 36356 ID=Msa1413020-mRNA-1:cds;Parent=Msa1413020-mRNA-1
tig0023989 CDS 34818 34906 34818 ID=Msa1413020-mRNA-1:cds;Parent=Msa1413020-mRNA-1
tig0023989 CDS 34231 34304 34231 ID=Msa1413020-mRNA-1:cds;Parent=Msa1413020-mRNA-1
tig0023989 CDS 34099 34149 34099 ID=Msa1413020-mRNA-1:cds;Parent=Msa1413020-mRNA-1
tig0023989 CDS 33936 34012 33936 ID=Msa1413020-mRNA-1:cds;Parent=Msa1413020-mRNA-1
tig0023989 CDS 33346 33818 33346 ID=Msa1413020-mRNA-1:cds;Parent=Msa1413020-mRNA-1
Gene Sequence

>Msa1413020

ATGGCTCGTGCTACTACTACAAATTCGAATAATAACTGGCTATCATTTTCACTTTCACCCATGGAAATGCCTCAGTTTGTTCAATATGACCAAGCTTCTTCCGTTACTACTAACTCCTCTCATCAAAATCATCATCACTATTTCCTTGACAACTTGTATACTCATAATGGGTGGGGAAACGGAACCAAAGCAGAAGAACAAGAACAATCAATCTGGTTTGTGGATTCATCAAACACTGTTAACCACACTCCACCACCAAAGCTAGAAGATTTTCTAGGTGACAGTCAAACAGAAACACAAGATTCATCATCACTCACAAACATGTATGATCATCATCACTACTTTGGTGGGGACCACTCCCAGCACCAGCACCAACACCAACACCACCAGGATCTTACTGGTTTTCAAGCTTTTTCAAACAACAACTCTGGCTCTGAAGTTGATGATTCTGGTTCAATAGGGAAATCAGGACAAGCTGCATGTGGGAATGAGTTTGGAACAACTCATTGTGTTGAGTCTGTGAATGAGTTTGCTGCTTACTCCACCGCTGCTGCGGCAGCGGTGGGGGCTAATGGAACTCTGTCGCTCGGTGTTGCGCAGAGTTCCGAGGAAAATGCTATTGTTGCTGTTGCTGCTGATTCTGATAGCTCTAAGAAGATTGTTGATACTTTTGGCCAAAGAACTTCAATTTACCGTGGTGTCACTAGGCATAGATGGACAGGAAGATATGAAGCCCATCTATGGGATAACAGCTGTAGAAGGGAAGGTCAAGCTAGGAAGGGTCGTCAAGGTGGATATGACAAAGAAGAAAAGGCCGCAAGATCCTATGATTTGGCTGCTCTAAAGTATTGGGGGCCCACTGCTACTACCAACTTCCCTGTTTCCAATTATGCAAAAGAATTGGAAGAGATGAAGAATGTAACAAAGCAAGAATTCATTGCTTCATTGAGAAGGAAGAGTAGTGGCTTCTCAAGAGGAGCTTCCATCTACCGGGGTGTCACAAGGCATCACCAACAGGGTAGGTGGCAAGCGAGAATTGGCCGTGTTGCCGGGAACAAAGACTTGTATCTCGGAACATTTGCAACTGAAGAGGAAGCTGCAGAGGCCTACGACATTGCGGCCATAAAGTTCCGAGGCGCAAATGCAGTGACAAACTTTGAGATGAACAGATATGACGTGGAAGCGATAATGAAGAGCTCTCTTCCAGTTGGCGGGGCAGCTAAACGCCTGAAGCGTTCTCTAGAATCAGAACAGAAAGCTTCTACTGACAACAACATTCAGCAGAACAACCCTCAACAAAGTGCTGCAAACATGACCAACAGCAGCAGCAGCAACAACATCAACTTCTCGGCCATTCAACATCAGCAACCAATGGCTTCAATCCCCTATGGTATTCCGTATGACTCGAATACGGCTTACTATCATCACAACCTCTTCCAGCATTTTCATGCTAGTACCAATGATGGAGCTGCAGAATCAGCAGCAAATGGAGGACTCAATGCCATGCCACCAACATCGGCTGAGTTCTATTTATGGCCAAATCAGTCCTATTGA
Protein sequence

>Msa1413020

MARATTTNSNNNWLSFSLSPMEMPQFVQYDQASSVTTNSSHQNHHHYFLDNLYTHNGWGNGTKAEEQEQSIWFVDSSNTVNHTPPPKLEDFLGDSQTETQDSSSLTNMYDHHHYFGGDHSQHQHQHQHHQDLTGFQAFSNNNSGSEVDDSGSIGKSGQAACGNEFGTTHCVESVNEFAAYSTAAAAAVGANGTLSLGVAQSSEENAIVAVAADSDSSKKIVDTFGQRTSIYRGVTRHRWTGRYEAHLWDNSCRREGQARKGRQGGYDKEEKAARSYDLAALKYWGPTATTNFPVSNYAKELEEMKNVTKQEFIASLRRKSSGFSRGASIYRGVTRHHQQGRWQARIGRVAGNKDLYLGTFATEEEAAEAYDIAAIKFRGANAVTNFEMNRYDVEAIMKSSLPVGGAAKRLKRSLESEQKASTDNNIQQNNPQQSAANMTNSSSSNNINFSAIQHQQPMASIPYGIPYDSNTAYYHHNLFQHFHASTNDGAAESAANGGLNAMPPTSAEFYLWPNQSY*