AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0553900


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0553900 MtrunA17_Chr8g0392721 71.795 39 11 0 66 104 12 50 1.36e-15 67.0
Msa0553900 MtrunA17_Chr1g0199791 66.000 50 16 1 12 61 1 49 4.30e-12 56.6
Msa0553900 MtrunA17_Chr3g0120701 72.727 33 9 0 66 98 12 44 8.64e-12 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 31 sgRNAs with CRISPR-Local

Find 145 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCGCAGCCTACTGCTGTT+GGG 0.324874 4_1:+51195547 Msa0553900:CDS
TTATTCTTCTCACCTCTTGC+CGG 0.378620 4_1:+51192947 Msa0553900:CDS
AACTTCTGTTTGTTCCTGTA+AGG 0.384138 4_1:+51195497 Msa0553900:CDS
TTGTCCACAACAGACCTTAC+AGG 0.423953 4_1:-51195511 None:intergenic
TTTCCGCAGCCTACTGCTGT+TGG 0.429558 4_1:+51195546 Msa0553900:CDS
TTGTGCGGCAGTTGCGGAGT+CGG 0.437749 4_1:-51193006 None:intergenic
ATAACCCTATGTGTTGTGTT+AGG 0.454244 4_1:-51192928 None:intergenic
TCCGCAGCCTACTGCTGTTG+GGG 0.456756 4_1:+51195548 Msa0553900:CDS
TGAACTCGGCGGCAGATCCA+TGG 0.464437 4_1:+51192877 Msa0553900:CDS
TCTTGGTGTGCAGTGTAGGC+TGG 0.481877 4_1:+51195460 Msa0553900:intron
ATCATCTCCTCACTGGAAAC+CGG 0.502488 4_1:+51192851 None:intergenic
TTGTTGTTGTGCGGCAGTTG+CGG 0.505008 4_1:-51193012 None:intergenic
TCTGCCGCCGAGTTCAGCGC+CGG 0.543138 4_1:-51192870 None:intergenic
CCGCAGCCTACTGCTGTTGG+GGG 0.543395 4_1:+51195549 Msa0553900:CDS
TGTTCCTGTAAGGTCTGTTG+TGG 0.543606 4_1:+51195507 Msa0553900:CDS
CTGGAAACCGGCGCTGAACT+CGG 0.546923 4_1:+51192863 Msa0553900:CDS
GGTGTGCAGTGTAGGCTGGA+GGG 0.577637 4_1:+51195464 Msa0553900:intron
GGCTGCGGAAACACAGACCT+GGG 0.579722 4_1:-51195534 None:intergenic
TCGGCGAGATGTGCGTGGAT+CGG 0.580720 4_1:-51192987 None:intergenic
ATGACCTTCGTAACTATCAC+CGG 0.588059 4_1:+51193035 Msa0553900:CDS
TGGTGTGCAGTGTAGGCTGG+AGG 0.595874 4_1:+51195463 Msa0553900:intron
AGGCTGCGGAAACACAGACC+TGG 0.597241 4_1:-51195535 None:intergenic
GAAGGTCATTTGTTGTTGTG+CGG 0.601943 4_1:-51193021 None:intergenic
CGGAGTCGGCGAGATGTGCG+TGG 0.602009 4_1:-51192992 None:intergenic
AGGACCTAACACAACACATA+GGG 0.603430 4_1:+51192924 Msa0553900:CDS
GAGGACCTAACACAACACAT+AGG 0.616752 4_1:+51192923 Msa0553900:CDS
AGGTCTGTTGTGGACAACCC+AGG 0.617858 4_1:+51195517 Msa0553900:CDS
AACGTCGAGACTCAAAGCCA+TGG 0.624136 4_1:-51192894 None:intergenic
GAGTCTCGACGTTCATAACG+AGG 0.648715 4_1:+51192904 Msa0553900:CDS
GTTACCGGTGATAGTTACGA+AGG 0.714605 4_1:-51193039 None:intergenic
GAAACCGGCGCTGAACTCGG+CGG 0.757139 4_1:+51192866 Msa0553900:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAATATATAAAAAGAAAT+TGG - chr4_1:51195444-51195463 None:intergenic 10.0%
!!! AATTTCTTTTTATATATTCT+TGG + chr4_1:51195443-51195462 Msa0553900:intron 10.0%
!! CTAAATAATTTCAACAAAAA+TGG - chr4_1:51193366-51193385 None:intergenic 15.0%
!! AATATTAAACAATTCTGAAT+TGG - chr4_1:51194280-51194299 None:intergenic 15.0%
!! TGTATTTCCTTAAATTTAAT+AGG + chr4_1:51194347-51194366 Msa0553900:intron 15.0%
!! TAATTTCGAATTTGACATTA+GGG + chr4_1:51193110-51193129 Msa0553900:intron 20.0%
!!! ATTTATGTTTTTCGAATAGT+AGG + chr4_1:51193392-51193411 Msa0553900:intron 20.0%
!!! TCTTAAAGCTTTTAAATAGA+TGG + chr4_1:51193770-51193789 Msa0553900:intron 20.0%
!! TGTGAAATTATCAAATTTGA+AGG + chr4_1:51193997-51194016 Msa0553900:intron 20.0%
!!! GTCATTTGAGTTAAAATATT+TGG + chr4_1:51194157-51194176 Msa0553900:intron 20.0%
!! TCCTTAAATTTAATAGGATT+TGG + chr4_1:51194353-51194372 Msa0553900:intron 20.0%
!!! GATTTTCAATATTTGCTTTA+TGG + chr4_1:51194730-51194749 Msa0553900:intron 20.0%
!!! TTTTTTTCTTGAATTGACTT+TGG + chr4_1:51194853-51194872 Msa0553900:intron 20.0%
!! ACAAATAAATGTATGTTATG+TGG - chr4_1:51194904-51194923 None:intergenic 20.0%
!! ACATAACATACATTTATTTG+TGG + chr4_1:51194903-51194922 Msa0553900:intron 20.0%
!! CATAACATACATTTATTTGT+GGG + chr4_1:51194904-51194923 Msa0553900:intron 20.0%
!! ATAACATACATTTATTTGTG+GGG + chr4_1:51194905-51194924 Msa0553900:intron 20.0%
!!! TGTAGTTACAATAAGATTTT+GGG + chr4_1:51195389-51195408 Msa0553900:intron 20.0%
! CTAATTTCGAATTTGACATT+AGG + chr4_1:51193109-51193128 Msa0553900:intron 25.0%
! CTCAAACAAGATGATAAAAA+AGG + chr4_1:51193420-51193439 Msa0553900:intron 25.0%
! AGAGTGAAATAAAATGGAAA+AGG - chr4_1:51193492-51193511 None:intergenic 25.0%
!!! CAAACTAACAAAACTTTTTC+TGG - chr4_1:51193729-51193748 None:intergenic 25.0%
!!! CAGAAAAAGTTTTGTTAGTT+TGG + chr4_1:51193727-51193746 Msa0553900:intron 25.0%
!!! CTATTTAAAAGCTTTAAGAC+AGG - chr4_1:51193769-51193788 None:intergenic 25.0%
! TTTCACAATGTCAAAATCAA+AGG - chr4_1:51193984-51194003 None:intergenic 25.0%
!!! AGTTTTGAGAAAATGACTTA+CGG - chr4_1:51194056-51194075 None:intergenic 25.0%
! TAAATGCACTAAGTTACTAT+TGG + chr4_1:51194207-51194226 Msa0553900:intron 25.0%
! GCCAAATCCTATTAAATTTA+AGG - chr4_1:51194357-51194376 None:intergenic 25.0%
! AATTTATAACCACTTTGATC+AGG - chr4_1:51194569-51194588 None:intergenic 25.0%
! CCTAAAAATCAAAGTTTGAT+CGG - chr4_1:51194600-51194619 None:intergenic 25.0%
!!! TTTTTCAATGCAAATCATGA+TGG - chr4_1:51194682-51194701 None:intergenic 25.0%
! CAACATAAGAAAACAATACA+TGG - chr4_1:51194711-51194730 None:intergenic 25.0%
! TCAATAACATGTAGAAATGA+TGG - chr4_1:51194784-51194803 None:intergenic 25.0%
! TGCTATCATATAATTTGATG+AGG - chr4_1:51194830-51194849 None:intergenic 25.0%
! ATAAATGTATGTTATGTGGA+TGG - chr4_1:51194900-51194919 None:intergenic 25.0%
! ATCAATTGATAAGAAATTGC+AGG - chr4_1:51195284-51195303 None:intergenic 25.0%
! TGCAATTTCTTATCAATTGA+TGG + chr4_1:51195283-51195302 Msa0553900:intron 25.0%
! ATATTTCGTGTATGAAATGT+TGG + chr4_1:51195323-51195342 Msa0553900:intron 25.0%
!! GTGTAGTTACAATAAGATTT+TGG + chr4_1:51195388-51195407 Msa0553900:intron 25.0%
!! GTAGTTACAATAAGATTTTG+GGG + chr4_1:51195390-51195409 Msa0553900:intron 25.0%
!! TAGTTACAATAAGATTTTGG+GGG + chr4_1:51195391-51195410 Msa0553900:intron 25.0%
CAGTCAATATGAACATGAAT+CGG - chr4_1:51193078-51193097 None:intergenic 30.0%
TAGCAGTTGCAATTTAACAT+AGG + chr4_1:51193271-51193290 Msa0553900:intron 30.0%
CTTGGTAGAGTGAAATAAAA+TGG - chr4_1:51193498-51193517 None:intergenic 30.0%
!! AGTTGTGATCTTTGAGTTTT+AGG + chr4_1:51193554-51193573 Msa0553900:intron 30.0%
!! GATCAGAAAGTATTGTAGTT+TGG + chr4_1:51193592-51193611 Msa0553900:intron 30.0%
TGACAATAACTATTTCCCTT+CGG + chr4_1:51193814-51193833 Msa0553900:intron 30.0%
GAATCATCAGAAAAATAAGG+CGG - chr4_1:51193869-51193888 None:intergenic 30.0%
!!! GCCTTATTTTTCTGATGATT+CGG + chr4_1:51193868-51193887 Msa0553900:intron 30.0%
ACCGAATCATCAGAAAAATA+AGG - chr4_1:51193872-51193891 None:intergenic 30.0%
ACATCTAAGAATTACCTCAA+TGG - chr4_1:51193900-51193919 None:intergenic 30.0%
GAGAATGATATGATACCATA+CGG - chr4_1:51193928-51193947 None:intergenic 30.0%
CAAACTGAAGGAAGTAAATT+AGG - chr4_1:51194450-51194469 None:intergenic 30.0%
CTAATTTACTTCCTTCAGTT+TGG + chr4_1:51194448-51194467 Msa0553900:intron 30.0%
TTGGGTTAATATACAACCTA+TGG + chr4_1:51194474-51194493 Msa0553900:intron 30.0%
AAATTGAATCCTGATCAAAG+TGG + chr4_1:51194557-51194576 Msa0553900:intron 30.0%
!! CCGATCAAACTTTGATTTTT+AGG + chr4_1:51194597-51194616 Msa0553900:intron 30.0%
! TCTAAGACAGAACAGAATAA+GGG - chr4_1:51195224-51195243 None:intergenic 30.0%
! ATCTAAGACAGAACAGAATA+AGG - chr4_1:51195225-51195244 None:intergenic 30.0%
ATAACCCTATGTGTTGTGTT+AGG - chr4_1:51192931-51192950 None:intergenic 35.0%
! GGTGAGAGAAATAGATTTTC+CGG - chr4_1:51192969-51192988 None:intergenic 35.0%
CAATATGAACATGAATCGGT+GGG - chr4_1:51193074-51193093 None:intergenic 35.0%
TCAATATGAACATGAATCGG+TGG - chr4_1:51193075-51193094 None:intergenic 35.0%
CAAAACAAAAGCTTGAAGCT+TGG - chr4_1:51193516-51193535 None:intergenic 35.0%
!!! AGCTTCAAGCTTTTGTTTTG+TGG + chr4_1:51193516-51193535 Msa0553900:intron 35.0%
GAGAGAATAGTAAATTCACC+TGG - chr4_1:51193707-51193726 None:intergenic 35.0%
TCTAAGAATTACCTCAATGG+TGG - chr4_1:51193897-51193916 None:intergenic 35.0%
GCTACAATGTCAAATAGCTA+TGG + chr4_1:51194019-51194038 Msa0553900:intron 35.0%
CTCTCAATCTCTTATTGTAG+TGG + chr4_1:51194074-51194093 Msa0553900:intron 35.0%
TGCTTATGATCACACAAATG+TGG - chr4_1:51194234-51194253 None:intergenic 35.0%
ATATTCTTCACTCTCTCCAT+AGG - chr4_1:51194493-51194512 None:intergenic 35.0%
GAATGCTTGCAAGAACAAAA+TGG - chr4_1:51194646-51194665 None:intergenic 35.0%
! CATTTATTTGTGGGGTTTTG+TGG + chr4_1:51194913-51194932 Msa0553900:intron 35.0%
CTGTGAAAAATTCTAGCAGA+TGG + chr4_1:51195106-51195125 Msa0553900:intron 35.0%
AAAGACAGACACAAAACACT+TGG - chr4_1:51195178-51195197 None:intergenic 35.0%
CTAAGACAGAACAGAATAAG+GGG - chr4_1:51195223-51195242 None:intergenic 35.0%
ATGAAATGTTGGACATGCTT+CGG + chr4_1:51195334-51195353 Msa0553900:intron 35.0%
CTAGTTCATATTCATACCCT+TGG - chr4_1:51195360-51195379 None:intergenic 35.0%
GGTATGAATATGAACTAGCA+TGG + chr4_1:51195362-51195381 Msa0553900:intron 35.0%
AACTTCTGTTTGTTCCTGTA+AGG + chr4_1:51195497-51195516 Msa0553900:CDS 35.0%
AGGACCTAACACAACACATA+GGG + chr4_1:51192924-51192943 Msa0553900:CDS 40.0%
TTATTCTTCTCACCTCTTGC+CGG + chr4_1:51192947-51192966 Msa0553900:CDS 40.0%
! GAAATAGATTTTCCGGCAAG+AGG - chr4_1:51192962-51192981 None:intergenic 40.0%
!! GAAGGTCATTTGTTGTTGTG+CGG - chr4_1:51193024-51193043 None:intergenic 40.0%
ATGACCTTCGTAACTATCAC+CGG + chr4_1:51193035-51193054 Msa0553900:CDS 40.0%
TAGTTTGGATTGAGTGCAGA+GGG + chr4_1:51193607-51193626 Msa0553900:intron 40.0%
AATAAGGCGGCTTCACAATT+AGG - chr4_1:51193856-51193875 None:intergenic 40.0%
TCTTAGATGTGCTTACCGTA+TGG + chr4_1:51193910-51193929 Msa0553900:intron 40.0%
GTAGTGGTTGAATAGCTTCA+AGG + chr4_1:51194090-51194109 Msa0553900:intron 40.0%
ACTTCCTTCAGTTTGGTTGT+TGG + chr4_1:51194455-51194474 Msa0553900:intron 40.0%
CTTCCTTCAGTTTGGTTGTT+GGG + chr4_1:51194456-51194475 Msa0553900:intron 40.0%
TAACCCAACAACCAAACTGA+AGG - chr4_1:51194462-51194481 None:intergenic 40.0%
! ATATTCTTGGTGTGCAGTGT+AGG + chr4_1:51195456-51195475 Msa0553900:intron 40.0%
GAGGACCTAACACAACACAT+AGG + chr4_1:51192923-51192942 Msa0553900:CDS 45.0%
GTTACCGGTGATAGTTACGA+AGG - chr4_1:51193042-51193061 None:intergenic 45.0%
ATCACAACTGTCACTCTGTC+AGG - chr4_1:51193543-51193562 None:intergenic 45.0%
GTAGTTTGGATTGAGTGCAG+AGG + chr4_1:51193606-51193625 Msa0553900:intron 45.0%
! CAGAGGGATGCTTTTGATGT+TGG + chr4_1:51193623-51193642 Msa0553900:intron 45.0%
! GGGATGCTTTTGATGTTGGA+CGG + chr4_1:51193627-51193646 Msa0553900:intron 45.0%
! CTGGCTCTCATATTGTTGTC+AGG - chr4_1:51193688-51193707 None:intergenic 45.0%
CTGACAACAATATGAGAGCC+AGG + chr4_1:51193686-51193705 Msa0553900:intron 45.0%
TTTCCAATGCACTCTCCGAA+GGG - chr4_1:51193832-51193851 None:intergenic 45.0%
! TTTTCCAATGCACTCTCCGA+AGG - chr4_1:51193833-51193852 None:intergenic 45.0%
!! TGATTCGGTTTCCACCATTG+AGG + chr4_1:51193883-51193902 Msa0553900:intron 45.0%
GCAGTGAACTGCATAACAAC+AGG - chr4_1:51194964-51194983 None:intergenic 45.0%
CACTGCGTGCATTTCTGTTA+TGG + chr4_1:51194978-51194997 Msa0553900:intron 45.0%
! ACCAGTTTTGCTGCTATGTG+TGG + chr4_1:51195012-51195031 Msa0553900:intron 45.0%
ACCACACATAGCAGCAAAAC+TGG - chr4_1:51195016-51195035 None:intergenic 45.0%
! TTTTGCTGCTATGTGTGGTC+TGG + chr4_1:51195017-51195036 Msa0553900:intron 45.0%
TTTGCTGCTATGTGTGGTCT+GGG + chr4_1:51195018-51195037 Msa0553900:intron 45.0%
TGTTCCTGTAAGGTCTGTTG+TGG + chr4_1:51195507-51195526 Msa0553900:CDS 45.0%
TTGTCCACAACAGACCTTAC+AGG - chr4_1:51195514-51195533 None:intergenic 45.0%
AACGTCGAGACTCAAAGCCA+TGG - chr4_1:51192897-51192916 None:intergenic 50.0%
GAGTCTCGACGTTCATAACG+AGG + chr4_1:51192904-51192923 Msa0553900:CDS 50.0%
! TTGTTGTTGTGCGGCAGTTG+CGG - chr4_1:51193015-51193034 None:intergenic 50.0%
GGTGGGAGAAAGTGAGTTAC+CGG - chr4_1:51193057-51193076 None:intergenic 50.0%
TGATGTTGGACGGTGTTCAG+CGG + chr4_1:51193637-51193656 Msa0553900:intron 50.0%
! TTTCCCTTCGGAGAGTGCAT+TGG + chr4_1:51193826-51193845 Msa0553900:intron 50.0%
!! GGGTTTTGTGGCGTTTGTCT+CGG + chr4_1:51194925-51194944 Msa0553900:intron 50.0%
TGGCGTTTGTCTCGGCTGTT+TGG + chr4_1:51194933-51194952 Msa0553900:intron 55.0%
GGCGTTTGTCTCGGCTGTTT+GGG + chr4_1:51194934-51194953 Msa0553900:intron 55.0%
CTATGTGTGGTCTGGGCATG+TGG + chr4_1:51195025-51195044 Msa0553900:intron 55.0%
TGTTGGACATGCTTCGGCCA+AGG + chr4_1:51195340-51195359 Msa0553900:intron 55.0%
GTTGGACATGCTTCGGCCAA+GGG + chr4_1:51195341-51195360 Msa0553900:intron 55.0%
! TCTTGGTGTGCAGTGTAGGC+TGG + chr4_1:51195460-51195479 Msa0553900:intron 55.0%
AGGTCTGTTGTGGACAACCC+AGG + chr4_1:51195517-51195536 Msa0553900:CDS 55.0%
TTTCCGCAGCCTACTGCTGT+TGG + chr4_1:51195546-51195565 Msa0553900:CDS 55.0%
TTCCGCAGCCTACTGCTGTT+GGG + chr4_1:51195547-51195566 Msa0553900:CDS 55.0%
GGGGGAGTAGGTGATGTTCA+TGG + chr4_1:51195567-51195586 Msa0553900:CDS 55.0%
! CTGGAAACCGGCGCTGAACT+CGG + chr4_1:51192863-51192882 Msa0553900:CDS 60.0%
TGAACTCGGCGGCAGATCCA+TGG + chr4_1:51192877-51192896 Msa0553900:CDS 60.0%
TCGGCGAGATGTGCGTGGAT+CGG - chr4_1:51192990-51193009 None:intergenic 60.0%
TTGTGCGGCAGTTGCGGAGT+CGG - chr4_1:51193009-51193028 None:intergenic 60.0%
TGGTGTGCAGTGTAGGCTGG+AGG + chr4_1:51195463-51195482 Msa0553900:intron 60.0%
GGTGTGCAGTGTAGGCTGGA+GGG + chr4_1:51195464-51195483 Msa0553900:intron 60.0%
GGCTGCGGAAACACAGACCT+GGG - chr4_1:51195537-51195556 None:intergenic 60.0%
AGGCTGCGGAAACACAGACC+TGG - chr4_1:51195538-51195557 None:intergenic 60.0%
TCCGCAGCCTACTGCTGTTG+GGG + chr4_1:51195548-51195567 Msa0553900:CDS 60.0%
CCTACTCCCCCAACAGCAGT+AGG - chr4_1:51195558-51195577 None:intergenic 60.0%
CCTACTGCTGTTGGGGGAGT+AGG + chr4_1:51195555-51195574 Msa0553900:CDS 60.0%
! GAAACCGGCGCTGAACTCGG+CGG + chr4_1:51192866-51192885 Msa0553900:CDS 65.0%
CCCCCAACAGCAGTAGGCTG+CGG - chr4_1:51195552-51195571 None:intergenic 65.0%
CCGCAGCCTACTGCTGTTGG+GGG + chr4_1:51195549-51195568 Msa0553900:CDS 65.0%
TCTGCCGCCGAGTTCAGCGC+CGG - chr4_1:51192873-51192892 None:intergenic 70.0%
CGGAGTCGGCGAGATGTGCG+TGG - chr4_1:51192995-51193014 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 51192863 51195611 51192863 ID=Msa0553900;Name=Msa0553900
chr4_1 mRNA 51192863 51195611 51192863 ID=Msa0553900-mRNA-1;Parent=Msa0553900;Name=Msa0553900-mRNA-1;_AED=0.69;_eAED=0.69;_QI=0|0|0|0.5|1|1|2|0|110
chr4_1 exon 51192863 51193056 51192863 ID=Msa0553900-mRNA-1:exon:14618;Parent=Msa0553900-mRNA-1
chr4_1 exon 51195473 51195611 51195473 ID=Msa0553900-mRNA-1:exon:14619;Parent=Msa0553900-mRNA-1
chr4_1 CDS 51192863 51193056 51192863 ID=Msa0553900-mRNA-1:cds;Parent=Msa0553900-mRNA-1
chr4_1 CDS 51195473 51195611 51195473 ID=Msa0553900-mRNA-1:cds;Parent=Msa0553900-mRNA-1
Gene Sequence

>Msa0553900

CTGGAAACCGGCGCTGAACTCGGCGGCAGATCCATGGCTTTGAGTCTCGACGTTCATAACGAGGACCTAACACAACACATAGGGTTATTCTTCTCACCTCTTGCCGGAAAATCTATTTCTCTCACCGATCCACGCACATCTCGCCGACTCCGCAACTGCCGCACAACAACAAATGACCTTCGTAACTATCACCGTGTAGGCTGGAGGGTGCTGTTAACAACTTCTGTTTGTTCCTGTAAGGTCTGTTGTGGACAACCCAGGTCTGTGTTTCCGCAGCCTACTGCTGTTGGGGGAGTAGGTGATGTTCATGGTTGTGTTCTTTTAGTTTTGTAA

Protein sequence

>Msa0553900

LETGAELGGRSMALSLDVHNEDLTQHIGLFFSPLAGKSISLTDPRTSRRLRNCRTTTNDLRNYHRVGWRVLLTTSVCSCKVCCGQPRSVFPQPTAVGGVGDVHGCVLLVL*