AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0572500

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0572500 MtrunA17_Chr8g0387921 96.487 427 8 2 1 425 1 422 0.0 846
Msa0572500 MtrunA17_Chr8g0347321 60.465 430 155 4 10 425 14 442 0.0 546
Msa0572500 MtrunA17_Chr6g0456691 41.706 422 225 5 8 422 12 419 2.66e-118 352
Msa0572500 MtrunA17_Chr8g0391481 41.129 372 206 9 60 425 11 375 2.96e-100 304
Msa0572500 MtrunA17_Chr5g0414061 42.776 353 189 8 76 422 93 438 4.26e-99 303
Msa0572500 MtrunA17_Chr5g0414041 44.944 356 185 7 73 422 83 433 5.98e-99 302
Msa0572500 MtrunA17_Chr5g0414091 42.330 352 192 6 77 422 85 431 6.88e-99 302
Msa0572500 MtrunA17_Chr3g0140371 42.090 354 192 6 77 425 18 363 1.98e-98 298
Msa0572500 MtrunA17_Chr5g0414051 39.196 398 221 10 32 422 37 420 6.60e-98 299
Msa0572500 MtrunA17_Chr7g0226991 41.972 355 194 7 77 425 57 405 1.61e-96 295
Msa0572500 MtrunA17_Chr2g0283851 42.192 365 191 9 69 423 47 401 1.03e-94 290
Msa0572500 MtrunA17_Chr6g0456681 41.096 365 198 7 66 422 53 408 4.38e-93 286
Msa0572500 MtrunA17_Chr6g0451321 38.120 383 210 9 56 422 86 457 1.40e-87 274
Msa0572500 MtrunA17_Chr1g0188191 35.128 390 235 9 45 422 198 581 4.80e-80 258
Msa0572500 MtrunA17_Chr6g0476041 34.181 354 219 8 77 422 167 514 3.12e-74 241
Msa0572500 MtrunA17_Chr7g0221281 40.417 240 135 5 185 422 3 236 4.17e-62 201
Msa0572500 MtrunA17_Chr1g0175671 32.506 403 217 14 41 418 133 505 2.05e-54 189
Msa0572500 MtrunA17_Chr7g0258851 33.149 362 198 13 76 418 247 583 2.85e-54 190
Msa0572500 MtrunA17_Chr7g0221271 43.655 197 99 3 12 203 16 205 6.89e-53 176
Msa0572500 MtrunA17_Chr5g0414081 41.709 199 109 4 230 422 2 199 1.41e-49 167
Msa0572500 MtrunA17_Chr7g0259711 30.833 360 219 12 77 423 91 433 3.24e-46 165
Msa0572500 MtrunA17_Chr4g0071391 28.256 407 242 15 39 423 69 447 1.03e-45 164
Msa0572500 MtrunA17_Chr5g0413271 30.508 354 210 13 76 418 144 472 8.33e-45 162
Msa0572500 MtrunA17_Chr1g0213281 30.056 356 216 10 77 418 175 511 1.72e-41 154
Msa0572500 MtrunA17_Chr7g0221291 44.937 158 80 1 12 169 21 171 1.83e-41 144
Msa0572500 MtrunA17_Chr3g0113671 28.771 358 233 10 77 424 106 451 4.19e-41 151
Msa0572500 MtrunA17_Chr8g0368461 29.894 378 200 15 76 418 177 524 8.10e-41 152
Msa0572500 MtrunA17_Chr4g0068111 29.508 366 222 14 73 423 110 454 3.97e-40 149
Msa0572500 MtrunA17_Chr7g0269991 29.213 356 209 12 77 418 137 463 7.10e-40 148
Msa0572500 MtrunA17_Chr8g0384421 26.817 399 250 14 41 422 99 472 1.06e-39 148
Msa0572500 MtrunA17_Chr4g0049421 27.424 361 237 9 77 425 48 395 1.16e-39 146
Msa0572500 MtrunA17_Chr8g0384151 29.805 359 209 12 76 422 89 416 4.13e-39 145
Msa0572500 MtrunA17_Chr4g0006991 26.575 365 234 11 73 422 82 427 3.91e-38 142
Msa0572500 MtrunA17_Chr5g0430371 28.378 370 205 10 77 418 58 395 4.77e-38 142
Msa0572500 MtrunA17_Chr8g0360521 28.926 363 209 15 77 418 71 405 8.03e-38 142
Msa0572500 MtrunA17_Chr2g0287901 28.093 388 238 14 55 423 73 438 1.34e-37 142
Msa0572500 MtrunA17_Chr3g0105301 28.986 345 225 8 77 417 43 371 7.54e-37 138
Msa0572500 MtrunA17_Chr3g0085081 29.201 363 215 11 69 424 23 350 1.59e-36 137
Msa0572500 MtrunA17_Chr3g0114101 27.273 352 232 10 77 418 89 426 3.66e-36 137
Msa0572500 MtrunA17_Chr7g0242701 26.667 420 270 13 22 422 27 427 5.50e-36 137
Msa0572500 MtrunA17_Chr4g0005491 26.121 379 236 16 66 422 51 407 6.88e-36 136
Msa0572500 MtrunA17_Chr3g0091031 28.375 363 218 11 69 424 23 350 1.96e-35 134
Msa0572500 MtrunA17_Chr1g0171171 25.532 423 248 15 19 422 1 375 2.18e-35 134
Msa0572500 MtrunA17_Chr3g0085071 28.255 361 217 10 69 422 38 363 6.49e-35 132
Msa0572500 MtrunA17_Chr7g0243301 27.123 365 238 14 74 424 61 411 5.41e-34 131
Msa0572500 MtrunA17_Chr1g0210771 27.066 351 218 13 76 418 58 378 9.07e-34 130
Msa0572500 MtrunA17_Chr6g0455901 26.087 437 258 15 17 422 14 416 9.79e-34 130
Msa0572500 MtrunA17_Chr5g0437601 26.761 355 230 12 77 418 83 420 1.42e-33 130
Msa0572500 MtrunA17_Chr5g0437291 27.151 372 237 13 69 424 160 513 1.71e-32 128
Msa0572500 MtrunA17_Chr4g0070341 25.341 367 236 12 72 422 127 471 2.10e-32 127
Msa0572500 MtrunA17_Chr3g0097891 29.745 353 210 12 77 423 31 351 5.57e-31 121
Msa0572500 MtrunA17_Chr2g0314801 29.775 356 207 13 76 422 32 353 1.24e-30 120
Msa0572500 MtrunA17_Chr3g0126021 25.745 369 215 15 77 423 129 460 1.45e-28 117
Msa0572500 MtrunA17_Chr4g0045941 26.346 353 226 9 74 423 30 351 1.48e-28 115
Msa0572500 MtrunA17_Chr2g0283581 28.249 354 213 13 71 418 36 354 1.51e-28 115
Msa0572500 MtrunA17_Chr2g0325481 27.350 351 214 13 74 418 47 362 3.33e-28 114
Msa0572500 MtrunA17_Chr4g0045951 25.000 380 247 10 51 423 15 363 1.16e-26 110
Msa0572500 MtrunA17_Chr5g0413851 26.989 352 219 12 77 423 42 360 1.40e-26 109
Msa0572500 MtrunA17_Chr2g0314791 26.124 356 216 10 77 423 29 346 5.26e-25 105
Msa0572500 MtrunA17_Chr4g0005481 24.932 365 229 12 77 422 108 446 1.86e-24 104
Msa0572500 MtrunA17_Chr4g0041011 25.852 352 218 10 74 418 44 359 1.61e-23 101
Msa0572500 MtrunA17_Chr7g0275501 65.278 72 20 2 350 421 2 68 1.87e-23 93.2
Msa0572500 MtrunA17_Chr1g0209921 29.583 240 148 7 76 310 43 266 1.31e-18 85.5
Msa0572500 MtrunA17_Chr1g0178081 26.172 256 170 8 26 270 8 255 1.35e-15 77.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0572500 AT4G11090.1 55.734 436 172 4 1 425 3 428 0.0 517
Msa0572500 AT4G23790.1 57.611 427 173 6 5 425 6 430 1.16e-177 503
Msa0572500 AT1G01430.1 53.211 436 186 9 5 424 16 449 3.67e-161 462
Msa0572500 AT4G01080.1 52.099 405 167 5 22 424 53 432 3.60e-155 446
Msa0572500 AT5G15890.1 46.724 351 175 6 73 422 183 522 1.91e-109 332
Msa0572500 AT5G15900.1 40.047 427 233 8 1 422 1 409 3.98e-105 318
Msa0572500 AT5G15900.2 40.047 427 233 8 1 422 20 428 7.53e-105 318
Msa0572500 AT3G02440.1 40.051 392 210 7 47 424 90 470 8.07e-103 314
Msa0572500 AT3G02440.2 40.051 392 210 7 47 424 98 478 1.04e-102 314
Msa0572500 AT1G70230.1 38.118 425 246 9 1 422 1 411 2.19e-102 311
Msa0572500 AT3G28150.1 37.097 372 219 6 57 422 48 410 4.47e-88 274
Msa0572500 AT5G51640.1 34.840 376 220 9 60 422 125 488 4.98e-78 251
Msa0572500 AT4G25360.1 33.586 396 240 10 41 422 138 524 2.60e-74 242
Msa0572500 AT4G25360.2 33.586 396 240 10 41 422 138 524 2.60e-74 242
Msa0572500 AT5G06700.1 31.622 370 202 11 77 423 255 596 8.51e-51 181
Msa0572500 AT3G12060.1 31.806 371 200 13 77 423 196 537 1.15e-50 179
Msa0572500 AT3G06080.2 31.868 364 211 13 77 423 103 446 3.06e-46 166
Msa0572500 AT5G49340.1 31.180 356 217 9 75 418 98 437 4.77e-46 165
Msa0572500 AT5G19160.1 31.492 362 218 12 77 423 99 445 2.62e-44 160
Msa0572500 AT5G20590.1 30.387 362 205 14 77 418 141 475 9.67e-44 159
Msa0572500 AT1G60790.1 31.608 367 198 15 77 418 190 528 1.67e-42 157
Msa0572500 AT2G14530.1 27.778 378 225 13 68 422 54 406 5.77e-40 148
Msa0572500 AT1G48880.1 29.805 359 201 12 76 419 114 436 1.21e-38 145
Msa0572500 AT5G06230.2 28.877 374 226 13 70 424 14 366 1.56e-38 143
Msa0572500 AT5G64020.1 28.000 375 218 11 77 422 57 408 2.12e-38 143
Msa0572500 AT5G64470.2 29.640 361 226 13 77 423 55 401 2.57e-38 143
Msa0572500 AT5G06230.1 28.877 374 226 13 70 424 55 407 3.11e-38 143
Msa0572500 AT5G64470.3 29.888 358 223 13 77 423 55 395 3.92e-38 142
Msa0572500 AT5G20680.6 28.418 373 207 15 77 422 118 457 4.17e-38 144
Msa0572500 AT2G40160.1 28.010 382 244 12 59 423 57 424 4.63e-38 143
Msa0572500 AT2G40160.2 28.382 377 239 12 59 418 57 419 5.80e-38 143
Msa0572500 AT5G20680.4 28.418 373 207 15 77 422 212 551 8.26e-38 144
Msa0572500 AT5G20680.1 28.418 373 207 15 77 422 212 551 8.26e-38 144
Msa0572500 AT5G20680.3 28.418 373 207 15 77 422 212 551 8.26e-38 144
Msa0572500 AT5G20680.5 28.418 373 207 15 77 422 212 551 8.26e-38 144
Msa0572500 AT3G62390.1 25.888 394 234 15 48 418 109 467 8.52e-38 143
Msa0572500 AT5G20680.2 28.418 373 207 15 77 422 194 533 1.08e-37 144
Msa0572500 AT5G58600.1 28.371 356 226 9 77 423 66 401 1.34e-37 141
Msa0572500 AT1G73140.1 27.441 379 214 15 65 418 59 401 1.72e-35 135
Msa0572500 AT3G11570.1 28.655 342 214 12 93 423 98 420 3.11e-35 135
Msa0572500 AT5G01360.2 26.230 366 229 15 73 419 32 375 6.14e-35 133
Msa0572500 AT5G01360.1 26.230 366 229 15 73 419 88 431 2.09e-34 133
Msa0572500 AT2G40320.1 25.412 425 268 15 14 418 23 418 2.16e-34 133
Msa0572500 AT2G30010.1 25.131 382 245 10 60 423 39 397 2.24e-34 132
Msa0572500 AT2G30010.2 25.326 383 243 11 60 423 82 440 3.78e-34 132
Msa0572500 AT3G54260.1 27.322 366 208 15 77 424 51 376 1.71e-33 129
Msa0572500 AT3G11030.1 26.857 350 235 10 77 417 106 443 2.10e-33 130
Msa0572500 AT2G30900.1 28.691 359 199 13 77 418 43 361 1.08e-32 127
Msa0572500 AT3G55990.1 23.773 387 270 11 48 424 114 485 1.19e-32 129
Msa0572500 AT2G40150.1 24.722 360 253 9 76 424 70 422 2.02e-31 124
Msa0572500 AT2G31110.2 28.647 377 225 12 50 418 18 358 2.44e-31 123
Msa0572500 AT2G38320.1 25.648 386 251 14 58 424 38 406 4.55e-31 123
Msa0572500 AT3G14850.2 29.014 355 211 14 77 423 34 355 3.81e-30 120
Msa0572500 AT2G34070.1 27.089 347 222 9 74 418 62 379 1.02e-29 119
Msa0572500 AT5G01620.3 26.630 368 222 15 76 422 117 457 1.98e-29 119
Msa0572500 AT3G06080.1 32.283 254 150 8 77 318 103 346 1.98e-29 117
Msa0572500 AT5G01620.2 26.630 368 222 15 76 422 109 449 2.05e-29 119
Msa0572500 AT5G01620.1 26.630 368 222 15 76 422 109 449 2.05e-29 119
Msa0572500 AT5G19160.2 32.927 246 145 8 77 311 99 335 2.06e-29 117
Msa0572500 AT1G29050.1 27.841 352 216 10 77 423 61 379 8.93e-29 116
Msa0572500 AT1G78710.1 26.158 367 208 15 76 424 37 358 4.29e-27 111
Msa0572500 AT2G42570.1 26.446 363 218 14 62 415 35 357 1.17e-26 110
Msa0572500 AT5G64470.4 29.104 268 172 10 77 333 55 315 1.17e-25 106
Msa0572500 AT5G64470.1 28.996 269 173 10 77 334 55 316 1.24e-25 106
Msa0572500 AT2G40160.3 28.409 264 171 7 59 312 57 312 6.56e-25 104
Msa0572500 AT2G38320.2 24.850 334 218 11 107 424 7 323 9.21e-25 104
Msa0572500 AT3G62390.2 25.256 293 189 9 48 325 109 386 2.32e-24 104
Msa0572500 AT2G37720.1 24.093 386 237 11 62 422 128 482 3.30e-24 105
Msa0572500 AT5G20590.2 28.458 253 149 9 77 312 141 378 4.42e-24 103
Msa0572500 AT5G58600.2 28.270 237 154 6 77 310 66 289 6.10e-23 98.6
Msa0572500 AT5G06230.3 28.517 263 162 9 70 315 55 308 1.06e-22 98.2
Msa0572500 AT2G31110.3 30.049 203 133 3 50 252 18 211 5.49e-21 92.4
Msa0572500 AT2G30900.2 30.806 211 130 6 77 285 43 239 2.52e-20 90.9
Msa0572500 AT1G78710.2 29.050 179 114 4 76 252 30 197 1.42e-16 80.5
Msa0572500 AT3G14850.1 26.855 283 166 14 149 423 3 252 1.94e-11 64.3

Find 91 sgRNAs with CRISPR-Local

Find 195 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAGTTTGAGGTGGGGTTT+GGG 0.248412 4_1:-75394812 None:intergenic
CATGTTTCATTTGTATAAAT+TGG 0.250767 4_1:-75395100 None:intergenic
ACTTGATGCGTGGTAAAGTA+TGG 0.254267 4_1:+75395245 Msa0572500:CDS
AAGAAGTTTGAGGTGGGGTT+TGG 0.275870 4_1:-75394813 None:intergenic
TCGACAAACTGGATAGTAAA+TGG 0.284885 4_1:+75396894 Msa0572500:CDS
CTATGAGAATGACACTATAT+TGG 0.295402 4_1:+75396988 Msa0572500:CDS
ACTTCAGTATGTCAGTTATT+TGG 0.298802 4_1:+75396798 Msa0572500:CDS
GGATAGATTGAGAATGGTTT+TGG 0.300507 4_1:-75394570 None:intergenic
CTTGCTCTGCACGCTTTCTA+AGG 0.308263 4_1:+75395309 Msa0572500:CDS
TGAAGGAAAGTGCCAGCTTT+TGG 0.308991 4_1:-75396771 None:intergenic
TGTTTGCATTGGTGCTTACC+TGG 0.316596 4_1:+75397430 Msa0572500:CDS
TCCATTTCACCTTCTTTAAT+CGG 0.319502 4_1:-75397205 None:intergenic
GGATAACAAAATCAGTTAAA+TGG 0.327061 4_1:+75397499 Msa0572500:exon
TGAGTTAGAAGAGTTTAAAA+AGG 0.345867 4_1:+75397261 Msa0572500:CDS
AACAAGAACCTGACAGATCT+AGG 0.351818 4_1:+75397031 Msa0572500:CDS
CTGAACATGGTTACGAGAAA+TGG 0.358145 4_1:-75395284 None:intergenic
TAAATTGGACCCAATGGGTT+TGG 0.401463 4_1:-75395085 None:intergenic
TATGAGAATGACACTATATT+GGG 0.402812 4_1:+75396989 Msa0572500:CDS
CAAAATCAAGCCCTTGTGAT+TGG 0.411626 4_1:-75397110 None:intergenic
TTTGGAGTTGGTGTTGCTTA+CGG 0.415872 4_1:-75394591 None:intergenic
TATGGACCCGGATGCCCATC+TGG 0.419716 4_1:-75397355 None:intergenic
TTTGAGACCAGATGGGCATC+CGG 0.423399 4_1:+75397348 Msa0572500:CDS
GGTTTGGGGTTCAGTGAGAG+TGG 0.428680 4_1:-75394797 None:intergenic
AAGGGTTGAAATTGCCTGTA+TGG 0.436352 4_1:-75397373 None:intergenic
TGAGCTATATCTCGACAAAC+TGG 0.441451 4_1:+75396883 Msa0572500:CDS
ACCGATTAAAGAAGGTGAAA+TGG 0.442228 4_1:+75397204 Msa0572500:CDS
CTCTTGGAATGACATCATAA+TGG 0.463666 4_1:+75397462 Msa0572500:CDS
GTTTGGGGTTCAGTGAGAGT+GGG 0.464045 4_1:-75394796 None:intergenic
TTTGAAGAGGGAGTTGTTGA+AGG 0.465492 4_1:-75394846 None:intergenic
TTTGTATAAATTGGACCCAA+TGG 0.465556 4_1:-75395091 None:intergenic
ATGTCATTCCAAGAGTCTAT+AGG 0.471038 4_1:-75397454 None:intergenic
TTGTATAAATTGGACCCAAT+GGG 0.480307 4_1:-75395090 None:intergenic
AAAGAGAAGAAGTTTGAGGT+GGG 0.480555 4_1:-75394819 None:intergenic
GATTACATTATTATTTCAAC+GGG 0.486220 4_1:+75396938 Msa0572500:CDS
GTTTGCATTGGTGCTTACCT+GGG 0.490210 4_1:+75397431 Msa0572500:CDS
CGATTACATTATTATTTCAA+CGG 0.495469 4_1:+75396937 Msa0572500:CDS
TACCTGGGCCTATAGACTCT+TGG 0.497198 4_1:+75397446 Msa0572500:CDS
TCAGAAAGAGAAGAAGTTTG+AGG 0.498283 4_1:-75394823 None:intergenic
TTGAGACCAGATGGGCATCC+GGG 0.502873 4_1:+75397349 Msa0572500:CDS
GCTTACGGATAGATTGAGAA+TGG 0.505838 4_1:-75394576 None:intergenic
ATGGGCATCCGGGTCCATAC+AGG 0.516143 4_1:+75397359 Msa0572500:CDS
GAAGTTTGAGGTGGGGTTTG+GGG 0.523244 4_1:-75394811 None:intergenic
TTATTATTTCAACGGGAAAG+TGG 0.527276 4_1:+75396945 Msa0572500:CDS
ATCAAGTTGAGAAACCTTTG+AGG 0.527808 4_1:-75395229 None:intergenic
ATAGGTAACTCACAATCTCT+AGG 0.532576 4_1:-75395199 None:intergenic
GATTGGGTACCAAACCCATT+GGG 0.533030 4_1:+75395076 Msa0572500:CDS
GACACTATATTGGGCTGTCA+CGG 0.534615 4_1:+75396998 Msa0572500:CDS
TTCAGACTGATTGTTTGCAT+TGG 0.536161 4_1:+75397419 Msa0572500:CDS
TAGCTAGAAATTACACAACT+TGG 0.538439 4_1:+75394497 None:intergenic
TTCCAAGAGTCTATAGGCCC+AGG 0.538902 4_1:-75397448 None:intergenic
GACATACTGAAGTTGTATGA+AGG 0.541369 4_1:-75396788 None:intergenic
AAGAGAAGAAGTTTGAGGTG+GGG 0.541698 4_1:-75394818 None:intergenic
GAAAATGGAGAGTGGTTTAG+CGG 0.544102 4_1:+75397160 Msa0572500:CDS
TCTGGTCTCAAAAGCGAAAG+TGG 0.551364 4_1:-75397337 None:intergenic
CAACTCTCTCCCACCGACAC+TGG 0.551711 4_1:+75394886 Msa0572500:CDS
CATAGTCTCTTCCAATCACA+AGG 0.551938 4_1:+75397099 Msa0572500:CDS
GAAAGAGAAGAAGTTTGAGG+TGG 0.556991 4_1:-75394820 None:intergenic
TGATTGGGTACCAAACCCAT+TGG 0.557892 4_1:+75395075 Msa0572500:CDS
AGTCAAACCCTAGATCTGTC+AGG 0.559564 4_1:-75397039 None:intergenic
GGTAAAGTATGGGCACTGAT+TGG 0.559712 4_1:+75395256 Msa0572500:CDS
GAGTGAGAAGTAACTTGATG+AGG 0.562325 4_1:-75394726 None:intergenic
ATAATGGAGATGGTTGTCAA+TGG 0.563525 4_1:+75397478 Msa0572500:CDS
CTTAGAAAGCGTGCAGAGCA+AGG 0.565769 4_1:-75395308 None:intergenic
AGAACTGAACCGATTAAAGA+AGG 0.566208 4_1:+75397196 Msa0572500:CDS
CTTGATGCGTGGTAAAGTAT+GGG 0.577745 4_1:+75395246 Msa0572500:CDS
ATAGCAGCTTCCACAAGGAA+TGG 0.582566 4_1:-75396824 None:intergenic
GCAATTTCAACCCTTTGCCA+AGG 0.584888 4_1:+75397381 Msa0572500:CDS
ACAAAGAAGAAGAAATAATG+AGG 0.585396 4_1:+75394668 Msa0572500:exon
CCTCATGCTAGTTGTGCATG+TGG 0.592160 4_1:+75397543 Msa0572500:three_prime_UTR
AGGGATACAAGTCCAAAAGC+TGG 0.595283 4_1:+75396759 Msa0572500:CDS
GTCTTAGTCTACCACGATGA+GGG 0.597331 4_1:+75396740 Msa0572500:CDS
ACAAGAACCTGACAGATCTA+GGG 0.597345 4_1:+75397032 Msa0572500:CDS
ATGTTACTGACCAGTGTCGG+TGG 0.597544 4_1:-75394896 None:intergenic
CTCATGCTAGTTGTGCATGT+GGG 0.605238 4_1:+75397544 Msa0572500:three_prime_UTR
TGTTACTGTTTCAACAAACA+CGG 0.607055 4_1:-75394628 None:intergenic
TGCAGAGCAAGGACTGAACA+TGG 0.608168 4_1:-75395297 None:intergenic
TATTTGGTCACCATTCCTTG+TGG 0.611002 4_1:+75396814 Msa0572500:CDS
GAATGACATCATAATGGAGA+TGG 0.612407 4_1:+75397468 Msa0572500:CDS
TGAAATTGCCTGTATGGACC+CGG 0.620052 4_1:-75397367 None:intergenic
AGGTTTCTCAACTTGATGCG+TGG 0.635087 4_1:+75395235 Msa0572500:CDS
AGGGAAGAAAACTAACTGCA+AGG 0.642801 4_1:-75397523 None:intergenic
CCACATGCACAACTAGCATG+AGG 0.651752 4_1:-75397543 None:intergenic
TGTTACTGACCAGTGTCGGT+GGG 0.657177 4_1:-75394895 None:intergenic
CACATGCACAACTAGCATGA+GGG 0.658468 4_1:-75397542 None:intergenic
TGTCTTAGTCTACCACGATG+AGG 0.661682 4_1:+75396739 Msa0572500:CDS
AATGGAGAGTGGTTTAGCGG+CGG 0.675176 4_1:+75397163 Msa0572500:CDS
TGTGTAGAGTTGGAAATGCA+TGG 0.692938 4_1:+75397688 Msa0572500:three_prime_UTR
CTTGGTTCTGATCTATGCTG+TGG 0.703009 4_1:+75394515 Msa0572500:five_prime_UTR
ATAGTCTCTTCCAATCACAA+GGG 0.713429 4_1:+75397100 Msa0572500:CDS
TGGACTTGTATCCCTCATCG+TGG 0.719158 4_1:-75396751 None:intergenic
AGAATGTTACTGACCAGTGT+CGG 0.728713 4_1:-75394899 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GATTTTTACATTTTTTTTTT+TGG + chr4_1:75395024-75395043 Msa0572500:intron 10.0%
!!! ATTTTATTTACAATATTTGA+TGG + chr4_1:75395916-75395935 Msa0572500:intron 10.0%
!!! TATAGTTTTATATTTCATTT+TGG + chr4_1:75397665-75397684 Msa0572500:three_prime_UTR 10.0%
!! ATTTAGATAAAAAATAGTGA+AGG - chr4_1:75396409-75396428 None:intergenic 15.0%
!!! TCTTCTATTTAAAACAAAAT+CGG - chr4_1:75396502-75396521 None:intergenic 15.0%
!!! AATCATATAAACTTTTACAA+TGG + chr4_1:75396667-75396686 Msa0572500:intron 15.0%
!!! GGTAAATGTGATTATTTTAA+TGG + chr4_1:75395052-75395071 Msa0572500:intron 20.0%
!!! TGATTATTTTAATGGTGATT+GGG + chr4_1:75395060-75395079 Msa0572500:CDS 20.0%
!! CATGTTTCATTTGTATAAAT+TGG - chr4_1:75395103-75395122 None:intergenic 20.0%
!! ATCAAAATTGCTTGAAAAAT+GGG + chr4_1:75395140-75395159 Msa0572500:CDS 20.0%
!!! ATATTGTTTTGAACTTTGAT+TGG + chr4_1:75395950-75395969 Msa0572500:intron 20.0%
!!! GTCTATACTTTTTCATTTTT+TGG - chr4_1:75396049-75396068 None:intergenic 20.0%
!!! AAAAATGAAAAAGTATAGAC+TGG + chr4_1:75396049-75396068 Msa0572500:intron 20.0%
!! TCAATTAAGAAGAGTTAAAA+AGG + chr4_1:75396094-75396113 Msa0572500:intron 20.0%
!! AAAAGGATAATGATTATGAA+AGG + chr4_1:75396111-75396130 Msa0572500:intron 20.0%
!! ATAATGATTATGAAAGGTAA+TGG + chr4_1:75396117-75396136 Msa0572500:intron 20.0%
!! TCAATTAAGAAGAGTTAAAA+AGG + chr4_1:75396094-75396113 Msa0572500:intron 20.0%
!! CGATTACATTATTATTTCAA+CGG + chr4_1:75396937-75396956 Msa0572500:CDS 20.0%
!! GATTACATTATTATTTCAAC+GGG + chr4_1:75396938-75396957 Msa0572500:CDS 20.0%
! ACAAAGAAGAAGAAATAATG+AGG + chr4_1:75394668-75394687 Msa0572500:exon 25.0%
!! ACTTGATGAGGTAATTTTTA+TGG - chr4_1:75394717-75394736 None:intergenic 25.0%
!!! ACATTTTTTTTTTTGGATGC+AGG + chr4_1:75395031-75395050 Msa0572500:intron 25.0%
!!! GTGATTATTTTAATGGTGAT+TGG + chr4_1:75395059-75395078 Msa0572500:CDS 25.0%
!!! AATTTTGATGTGATTCAATG+AGG - chr4_1:75395129-75395148 None:intergenic 25.0%
! CATCAAAATTGCTTGAAAAA+TGG + chr4_1:75395139-75395158 Msa0572500:CDS 25.0%
!!! GTCTTTTTTACGTGTTTTTT+CGG + chr4_1:75395480-75395499 Msa0572500:intron 25.0%
! CATATAAGCTGTAATAGAAT+AGG + chr4_1:75395636-75395655 Msa0572500:intron 25.0%
! ATGAAGTAATTAATCAACCT+AGG + chr4_1:75395689-75395708 Msa0572500:intron 25.0%
! AAAAGGATAATGATCATGAA+AGG + chr4_1:75396217-75396236 Msa0572500:intron 25.0%
! ATAATGATCATGAAAGGTAA+TGG + chr4_1:75396223-75396242 Msa0572500:intron 25.0%
!!! GCTATTTTTGAAGACATAAA+CGG + chr4_1:75396842-75396861 Msa0572500:CDS 25.0%
! TATGAGAATGACACTATATT+GGG + chr4_1:75396989-75397008 Msa0572500:CDS 25.0%
! TGAGTTAGAAGAGTTTAAAA+AGG + chr4_1:75397261-75397280 Msa0572500:CDS 25.0%
! GGATAACAAAATCAGTTAAA+TGG + chr4_1:75397499-75397518 Msa0572500:exon 25.0%
TGTTACTGTTTCAACAAACA+CGG - chr4_1:75394631-75394650 None:intergenic 30.0%
!! GTTCTTGTTCATTTTGAAGA+GGG - chr4_1:75394861-75394880 None:intergenic 30.0%
!! TGTTCTTGTTCATTTTGAAG+AGG - chr4_1:75394862-75394881 None:intergenic 30.0%
GAAATAGTGTTTAATTCCTG+TGG + chr4_1:75394944-75394963 Msa0572500:intron 30.0%
TTTCCAAAACAAGTTAACGT+TGG + chr4_1:75395001-75395020 Msa0572500:intron 30.0%
TTCCAAAACAAGTTAACGTT+GGG + chr4_1:75395002-75395021 Msa0572500:intron 30.0%
TTGTATAAATTGGACCCAAT+GGG - chr4_1:75395093-75395112 None:intergenic 30.0%
TTTGTATAAATTGGACCCAA+TGG - chr4_1:75395094-75395113 None:intergenic 30.0%
!!! AGATTTTTTGTACTGGAAGT+GGG + chr4_1:75395174-75395193 Msa0572500:CDS 30.0%
!!! TACCTATTTTTGATCCTCAA+AGG + chr4_1:75395215-75395234 Msa0572500:CDS 30.0%
! AACCTTTGAGGATCAAAAAT+AGG - chr4_1:75395220-75395239 None:intergenic 30.0%
CTCAAAGAAACATTCACAAA+AGG - chr4_1:75395448-75395467 None:intergenic 30.0%
! CTTTTGTGAATGTTTCTTTG+AGG + chr4_1:75395446-75395465 Msa0572500:intron 30.0%
AGAATAACTCAAGAATCTGT+TGG + chr4_1:75395571-75395590 Msa0572500:intron 30.0%
CTAGCTCAATAGATAAGATA+AGG + chr4_1:75395783-75395802 Msa0572500:intron 30.0%
! CTTCTTTCCTCAATTACTTT+TGG - chr4_1:75395895-75395914 None:intergenic 30.0%
AAATGCATAATTGACTAGAC+CGG - chr4_1:75396297-75396316 None:intergenic 30.0%
TGTACATGAAGAATAAACGA+GGG - chr4_1:75396371-75396390 None:intergenic 30.0%
! ATTTAAAACAAAATCGGCGA+AGG - chr4_1:75396496-75396515 None:intergenic 30.0%
! TTTGTCAATTTTGTGAAACC+TGG + chr4_1:75396592-75396611 Msa0572500:intron 30.0%
ACTTCAGTATGTCAGTTATT+TGG + chr4_1:75396798-75396817 Msa0572500:CDS 30.0%
TTATTATTTCAACGGGAAAG+TGG + chr4_1:75396945-75396964 Msa0572500:CDS 30.0%
CTATGAGAATGACACTATAT+TGG + chr4_1:75396988-75397007 Msa0572500:CDS 30.0%
TCCATTTCACCTTCTTTAAT+CGG - chr4_1:75397208-75397227 None:intergenic 30.0%
! TGGAAATTAAGCTTTTGCTT+AGG + chr4_1:75397224-75397243 Msa0572500:CDS 30.0%
! AACTTTAGCATTTTTGTCCT+TGG - chr4_1:75397401-75397420 None:intergenic 30.0%
!!! ATTGAGAATGGTTTTGGTTG+GGG - chr4_1:75394567-75394586 None:intergenic 35.0%
!!! GATTGAGAATGGTTTTGGTT+GGG - chr4_1:75394568-75394587 None:intergenic 35.0%
!!! AGATTGAGAATGGTTTTGGT+TGG - chr4_1:75394569-75394588 None:intergenic 35.0%
! GGATAGATTGAGAATGGTTT+TGG - chr4_1:75394573-75394592 None:intergenic 35.0%
CAACACCAACTCCAAAAAAT+CGG + chr4_1:75394598-75394617 Msa0572500:five_prime_UTR 35.0%
! AAAGAGAAGAAGTTTGAGGT+GGG - chr4_1:75394822-75394841 None:intergenic 35.0%
TCAGAAAGAGAAGAAGTTTG+AGG - chr4_1:75394826-75394845 None:intergenic 35.0%
TAATTGAACATTGAGACCAC+AGG - chr4_1:75394963-75394982 None:intergenic 35.0%
GGTCTCAATGTTCAATTAGT+CGG + chr4_1:75394965-75394984 Msa0572500:intron 35.0%
!!! ATCCCAACGTTAACTTGTTT+TGG - chr4_1:75395007-75395026 None:intergenic 35.0%
CAGTACAAAAAATCTCTGTC+TGG - chr4_1:75395169-75395188 None:intergenic 35.0%
!!! CAGACAGAGATTTTTTGTAC+TGG + chr4_1:75395167-75395186 Msa0572500:CDS 35.0%
!!! GAGATTTTTTGTACTGGAAG+TGG + chr4_1:75395173-75395192 Msa0572500:CDS 35.0%
ATAGGTAACTCACAATCTCT+AGG - chr4_1:75395202-75395221 None:intergenic 35.0%
!! ATCAAGTTGAGAAACCTTTG+AGG - chr4_1:75395232-75395251 None:intergenic 35.0%
GACAACAACGATGAATACAA+TGG - chr4_1:75395365-75395384 None:intergenic 35.0%
CATCGTTGTTGTCAATAGAT+TGG + chr4_1:75395372-75395391 Msa0572500:intron 35.0%
CACAAAAGGTCAGACAAAAA+AGG - chr4_1:75395434-75395453 None:intergenic 35.0%
AAACTACCAAAGTTATGCGT+TGG - chr4_1:75395536-75395555 None:intergenic 35.0%
GGTAGTTTATTTATCGAGTC+TGG + chr4_1:75395548-75395567 Msa0572500:intron 35.0%
ATAGAATAGGTCTATGCTCT+TGG + chr4_1:75395649-75395668 Msa0572500:intron 35.0%
AGAATATAGCACCTGAGAAT+CGG + chr4_1:75395826-75395845 Msa0572500:intron 35.0%
ATCAGCTCCAAAAGTAATTG+AGG + chr4_1:75395885-75395904 Msa0572500:intron 35.0%
!!! TTTTCAAGTTTTGCCAGTCT+AGG - chr4_1:75396065-75396084 None:intergenic 35.0%
GTGTACATGAAGAATAAACG+AGG - chr4_1:75396372-75396391 None:intergenic 35.0%
TTCAATTCAGTCCAAACAAG+TGG - chr4_1:75396627-75396646 None:intergenic 35.0%
!!! CTTGAACTATTTTGACCTTC+AGG + chr4_1:75396706-75396725 Msa0572500:intron 35.0%
GACATACTGAAGTTGTATGA+AGG - chr4_1:75396791-75396810 None:intergenic 35.0%
TCGACAAACTGGATAGTAAA+TGG + chr4_1:75396894-75396913 Msa0572500:CDS 35.0%
ATAGTCTCTTCCAATCACAA+GGG + chr4_1:75397100-75397119 Msa0572500:CDS 35.0%
! ATTTTCAAAATGGTCAGGTG+TGG - chr4_1:75397146-75397165 None:intergenic 35.0%
!! ACACCTGACCATTTTGAAAA+TGG + chr4_1:75397145-75397164 Msa0572500:CDS 35.0%
! TCTCCATTTTCAAAATGGTC+AGG - chr4_1:75397151-75397170 None:intergenic 35.0%
!! ACCATTTTGAAAATGGAGAG+TGG + chr4_1:75397152-75397171 Msa0572500:CDS 35.0%
! ACCACTCTCCATTTTCAAAA+TGG - chr4_1:75397156-75397175 None:intergenic 35.0%
AGAACTGAACCGATTAAAGA+AGG + chr4_1:75397196-75397215 Msa0572500:CDS 35.0%
ACCGATTAAAGAAGGTGAAA+TGG + chr4_1:75397204-75397223 Msa0572500:CDS 35.0%
! GCGTCTAAAGCTTCAAAAAA+TGG + chr4_1:75397286-75397305 Msa0572500:CDS 35.0%
TTCAGACTGATTGTTTGCAT+TGG + chr4_1:75397419-75397438 Msa0572500:CDS 35.0%
ATGTCATTCCAAGAGTCTAT+AGG - chr4_1:75397457-75397476 None:intergenic 35.0%
CTCTTGGAATGACATCATAA+TGG + chr4_1:75397462-75397481 Msa0572500:CDS 35.0%
! GAATGACATCATAATGGAGA+TGG + chr4_1:75397468-75397487 Msa0572500:CDS 35.0%
! ATAATGGAGATGGTTGTCAA+TGG + chr4_1:75397478-75397497 Msa0572500:CDS 35.0%
!! TTCATTTTGGTGTGTAGAGT+TGG + chr4_1:75397678-75397697 Msa0572500:three_prime_UTR 35.0%
GCTTACGGATAGATTGAGAA+TGG - chr4_1:75394579-75394598 None:intergenic 40.0%
!! TTTGGAGTTGGTGTTGCTTA+CGG - chr4_1:75394594-75394613 None:intergenic 40.0%
!!! AGACACCGATTTTTTGGAGT+TGG - chr4_1:75394606-75394625 None:intergenic 40.0%
!!! ACGGAAAGACACCGATTTTT+TGG - chr4_1:75394612-75394631 None:intergenic 40.0%
GAGTGAGAAGTAACTTGATG+AGG - chr4_1:75394729-75394748 None:intergenic 40.0%
! AAGAGAAGAAGTTTGAGGTG+GGG - chr4_1:75394821-75394840 None:intergenic 40.0%
GAAAGAGAAGAAGTTTGAGG+TGG - chr4_1:75394823-75394842 None:intergenic 40.0%
TTTGAAGAGGGAGTTGTTGA+AGG - chr4_1:75394849-75394868 None:intergenic 40.0%
AGAATGTTACTGACCAGTGT+CGG - chr4_1:75394902-75394921 None:intergenic 40.0%
! TAAATTGGACCCAATGGGTT+TGG - chr4_1:75395088-75395107 None:intergenic 40.0%
ACTTGATGCGTGGTAAAGTA+TGG + chr4_1:75395245-75395264 Msa0572500:CDS 40.0%
CTTGATGCGTGGTAAAGTAT+GGG + chr4_1:75395246-75395265 Msa0572500:CDS 40.0%
CTGAACATGGTTACGAGAAA+TGG - chr4_1:75395287-75395306 None:intergenic 40.0%
GTTGGAACCAAAACAAGATC+CGG - chr4_1:75395518-75395537 None:intergenic 40.0%
TTGGTTCCAACGCATAACTT+TGG + chr4_1:75395527-75395546 Msa0572500:intron 40.0%
TCAACCTAGGATGAAAAGAG+TGG + chr4_1:75395702-75395721 Msa0572500:intron 40.0%
! TTCTCCACTCTTTTCATCCT+AGG - chr4_1:75395709-75395728 None:intergenic 40.0%
!!! ACAACAACTCTGGGATTTTG+AGG + chr4_1:75395854-75395873 Msa0572500:intron 40.0%
TACGTGTATGGACTTGTAAC+TGG - chr4_1:75396265-75396284 None:intergenic 40.0%
AACAAGTGGTCAAAAAGTCC+AGG - chr4_1:75396613-75396632 None:intergenic 40.0%
! GGACTTTTTGACCACTTGTT+TGG + chr4_1:75396613-75396632 Msa0572500:intron 40.0%
GTTTGGACTGAATTGAAGCA+TGG + chr4_1:75396630-75396649 Msa0572500:intron 40.0%
!! AGACAGCTTTTTCTACCTGA+AGG - chr4_1:75396724-75396743 None:intergenic 40.0%
TATTTGGTCACCATTCCTTG+TGG + chr4_1:75396814-75396833 Msa0572500:CDS 40.0%
CAAAAATAGCAGCTTCCACA+AGG - chr4_1:75396832-75396851 None:intergenic 40.0%
TGAGCTATATCTCGACAAAC+TGG + chr4_1:75396883-75396902 Msa0572500:CDS 40.0%
AACAAGAACCTGACAGATCT+AGG + chr4_1:75397031-75397050 Msa0572500:CDS 40.0%
ACAAGAACCTGACAGATCTA+GGG + chr4_1:75397032-75397051 Msa0572500:CDS 40.0%
CATAGTCTCTTCCAATCACA+AGG + chr4_1:75397099-75397118 Msa0572500:CDS 40.0%
CAAAATCAAGCCCTTGTGAT+TGG - chr4_1:75397113-75397132 None:intergenic 40.0%
! ACAAGGGCTTGATTTTGTTC+AGG + chr4_1:75397116-75397135 Msa0572500:CDS 40.0%
GAAAATGGAGAGTGGTTTAG+CGG + chr4_1:75397160-75397179 Msa0572500:CDS 40.0%
!!! TTTCGCTTTTGAGACCAGAT+GGG + chr4_1:75397341-75397360 Msa0572500:CDS 40.0%
AAGGGTTGAAATTGCCTGTA+TGG - chr4_1:75397376-75397395 None:intergenic 40.0%
! GCATTTTTGTCCTTGGCAAA+GGG - chr4_1:75397394-75397413 None:intergenic 40.0%
! AGCATTTTTGTCCTTGGCAA+AGG - chr4_1:75397395-75397414 None:intergenic 40.0%
AGGGAAGAAAACTAACTGCA+AGG - chr4_1:75397526-75397545 None:intergenic 40.0%
!! CTTGGTTCTGATCTATGCTG+TGG + chr4_1:75394515-75394534 Msa0572500:five_prime_UTR 45.0%
! AGAAGTTTGAGGTGGGGTTT+GGG - chr4_1:75394815-75394834 None:intergenic 45.0%
! AAGAAGTTTGAGGTGGGGTT+TGG - chr4_1:75394816-75394835 None:intergenic 45.0%
TGATTGGGTACCAAACCCAT+TGG + chr4_1:75395075-75395094 Msa0572500:CDS 45.0%
GATTGGGTACCAAACCCATT+GGG + chr4_1:75395076-75395095 Msa0572500:CDS 45.0%
AGGTTTCTCAACTTGATGCG+TGG + chr4_1:75395235-75395254 Msa0572500:CDS 45.0%
!! GGTAAAGTATGGGCACTGAT+TGG + chr4_1:75395256-75395275 Msa0572500:CDS 45.0%
ATAGCACCTGAGAATCGGAT+TGG + chr4_1:75395831-75395850 Msa0572500:intron 45.0%
TGTTGTCCAATCCGATTCTC+AGG - chr4_1:75395840-75395859 None:intergenic 45.0%
! ATCGGATTGGACAACAACTC+TGG + chr4_1:75395844-75395863 Msa0572500:intron 45.0%
! TCGGATTGGACAACAACTCT+GGG + chr4_1:75395845-75395864 Msa0572500:intron 45.0%
GTAATGGCTTGAACAAGCTG+AGG + chr4_1:75396133-75396152 Msa0572500:intron 45.0%
GTAATGGCTTGAACAAGCTG+AGG + chr4_1:75396133-75396152 Msa0572500:intron 45.0%
CATACACGTATAGTTGCCAC+CGG + chr4_1:75396275-75396294 Msa0572500:intron 45.0%
TGCATAATTGACTAGACCGG+TGG - chr4_1:75396294-75396313 None:intergenic 45.0%
TGTCTTAGTCTACCACGATG+AGG + chr4_1:75396739-75396758 Msa0572500:CDS 45.0%
GTCTTAGTCTACCACGATGA+GGG + chr4_1:75396740-75396759 Msa0572500:CDS 45.0%
AGGGATACAAGTCCAAAAGC+TGG + chr4_1:75396759-75396778 Msa0572500:CDS 45.0%
!!! TGAAGGAAAGTGCCAGCTTT+TGG - chr4_1:75396774-75396793 None:intergenic 45.0%
ATAGCAGCTTCCACAAGGAA+TGG - chr4_1:75396827-75396846 None:intergenic 45.0%
GACACTATATTGGGCTGTCA+CGG + chr4_1:75396998-75397017 Msa0572500:CDS 45.0%
AGTCAAACCCTAGATCTGTC+AGG - chr4_1:75397042-75397061 None:intergenic 45.0%
TCTGGTCTCAAAAGCGAAAG+TGG - chr4_1:75397340-75397359 None:intergenic 45.0%
!!! CTTTCGCTTTTGAGACCAGA+TGG + chr4_1:75397340-75397359 Msa0572500:CDS 45.0%
TGAAATTGCCTGTATGGACC+CGG - chr4_1:75397370-75397389 None:intergenic 45.0%
GCAATTTCAACCCTTTGCCA+AGG + chr4_1:75397381-75397400 Msa0572500:CDS 45.0%
! TGTTTGCATTGGTGCTTACC+TGG + chr4_1:75397430-75397449 Msa0572500:CDS 45.0%
! GTTTGCATTGGTGCTTACCT+GGG + chr4_1:75397431-75397450 Msa0572500:CDS 45.0%
CACATGCACAACTAGCATGA+GGG - chr4_1:75397545-75397564 None:intergenic 45.0%
CTCATGCTAGTTGTGCATGT+GGG + chr4_1:75397544-75397563 Msa0572500:three_prime_UTR 45.0%
! GTTTGGGGTTCAGTGAGAGT+GGG - chr4_1:75394799-75394818 None:intergenic 50.0%
! GAAGTTTGAGGTGGGGTTTG+GGG - chr4_1:75394814-75394833 None:intergenic 50.0%
!! TGTTACTGACCAGTGTCGGT+GGG - chr4_1:75394898-75394917 None:intergenic 50.0%
! ATGTTACTGACCAGTGTCGG+TGG - chr4_1:75394899-75394918 None:intergenic 50.0%
TGCAGAGCAAGGACTGAACA+TGG - chr4_1:75395300-75395319 None:intergenic 50.0%
! CTTAGAAAGCGTGCAGAGCA+AGG - chr4_1:75395311-75395330 None:intergenic 50.0%
CTTGCTCTGCACGCTTTCTA+AGG + chr4_1:75395309-75395328 Msa0572500:CDS 50.0%
!! TTTTCGGCTGATGCGTTGAC+CGG + chr4_1:75395496-75395515 Msa0572500:intron 50.0%
!! GCGTTGACCGGATCTTGTTT+TGG + chr4_1:75395508-75395527 Msa0572500:intron 50.0%
GCCAGTTACAAGTCCTAGAC+TGG + chr4_1:75396155-75396174 Msa0572500:intron 50.0%
! GCCAGTCTAGGACTTGTAAC+TGG - chr4_1:75396159-75396178 None:intergenic 50.0%
CGGTGGCAACTATACGTGTA+TGG - chr4_1:75396277-75396296 None:intergenic 50.0%
TGGACTTGTATCCCTCATCG+TGG - chr4_1:75396754-75396773 None:intergenic 50.0%
AATGGAGAGTGGTTTAGCGG+CGG + chr4_1:75397163-75397182 Msa0572500:CDS 50.0%
! TTTGAGACCAGATGGGCATC+CGG + chr4_1:75397348-75397367 Msa0572500:CDS 50.0%
TACCTGGGCCTATAGACTCT+TGG + chr4_1:75397446-75397465 Msa0572500:CDS 50.0%
TTCCAAGAGTCTATAGGCCC+AGG - chr4_1:75397451-75397470 None:intergenic 50.0%
CCACATGCACAACTAGCATG+AGG - chr4_1:75397546-75397565 None:intergenic 50.0%
CCTCATGCTAGTTGTGCATG+TGG + chr4_1:75397543-75397562 Msa0572500:three_prime_UTR 50.0%
! GGTTTGGGGTTCAGTGAGAG+TGG - chr4_1:75394800-75394819 None:intergenic 55.0%
TTGAGACCAGATGGGCATCC+GGG + chr4_1:75397349-75397368 Msa0572500:CDS 55.0%
CAACTCTCTCCCACCGACAC+TGG + chr4_1:75394886-75394905 Msa0572500:CDS 60.0%
TATGGACCCGGATGCCCATC+TGG - chr4_1:75397358-75397377 None:intergenic 60.0%
ATGGGCATCCGGGTCCATAC+AGG + chr4_1:75397359-75397378 Msa0572500:CDS 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 75394500 75397707 75394500 ID=Msa0572500;Name=Msa0572500
chr4_1 mRNA 75394500 75397707 75394500 ID=Msa0572500-mRNA-1;Parent=Msa0572500;Name=Msa0572500-mRNA-1;_AED=0.08;_eAED=0.08;_QI=185|1|1|1|1|1|3|203|425
chr4_1 exon 75394500 75394907 75394500 ID=Msa0572500-mRNA-1:exon:26124;Parent=Msa0572500-mRNA-1
chr4_1 exon 75395053 75395330 75395053 ID=Msa0572500-mRNA-1:exon:26125;Parent=Msa0572500-mRNA-1
chr4_1 exon 75396728 75397707 75396728 ID=Msa0572500-mRNA-1:exon:26126;Parent=Msa0572500-mRNA-1
chr4_1 five_prime_UTR 75394500 75394684 75394500 ID=Msa0572500-mRNA-1:five_prime_utr;Parent=Msa0572500-mRNA-1
chr4_1 CDS 75394685 75394907 75394685 ID=Msa0572500-mRNA-1:cds;Parent=Msa0572500-mRNA-1
chr4_1 CDS 75395053 75395330 75395053 ID=Msa0572500-mRNA-1:cds;Parent=Msa0572500-mRNA-1
chr4_1 CDS 75396728 75397504 75396728 ID=Msa0572500-mRNA-1:cds;Parent=Msa0572500-mRNA-1
chr4_1 three_prime_UTR 75397505 75397707 75397505 ID=Msa0572500-mRNA-1:three_prime_utr;Parent=Msa0572500-mRNA-1
Gene Sequence

>Msa0572500

ATGAGGATCGATTCTTCTTTCAATTTCAACCATAAAAATTACCTCATCAAGTTACTTCTCACTCTTTTCTTCTCTGTACTCGCTTTTCGTCTTCTCTTCTTTCAATCTCTTCCCACTCTCACTGAACCCCAAACCCCACCTCAAACTTCTTCTCTTTCTGACCTTCAACAACTCCCTCTTCAAAATGAACAAGAACAAGATCAACTCTCTCCCACCGACACTGGTAAATGTGATTATTTTAATGGTGATTGGGTACCAAACCCATTGGGTCCAATTTATACAAATGAAACATGTGACCTCATTGAATCACATCAAAATTGCTTGAAAAATGGGAGACCAGACAGAGATTTTTTGTACTGGAAGTGGGCTCCTAGAGATTGTGAGTTACCTATTTTTGATCCTCAAAGGTTTCTCAACTTGATGCGTGGTAAAGTATGGGCACTGATTGGTGATTCCATTTCTCGTAACCATGTTCAGTCCTTGCTCTGCACGCTTTCTAAGGTAGAAAAAGCTGTCTTAGTCTACCACGATGAGGGATACAAGTCCAAAAGCTGGCACTTTCCTTCATACAACTTCAGTATGTCAGTTATTTGGTCACCATTCCTTGTGGAAGCTGCTATTTTTGAAGACATAAACGGCGTTTCAAGTTCTGAAGTTGAGCTATATCTCGACAAACTGGATAGTAAATGGACAGATCAGTATCTGAACTTCGATTACATTATTATTTCAACGGGAAAGTGGTTTGTCAAATCTGCAATCTACTATGAGAATGACACTATATTGGGCTGTCACGGCTGTTCAAGTAACAAGAACCTGACAGATCTAGGGTTTGACTTTGCATATCGCAAAGTCTTGAAAAATGTCATGAACTTCATAGTCTCTTCCAATCACAAGGGCTTGATTTTGTTCAGGACATCCACACCTGACCATTTTGAAAATGGAGAGTGGTTTAGCGGCGGAACGTGCAACAGAACTGAACCGATTAAAGAAGGTGAAATGGAAATTAAGCTTTTGCTTAGGATTCTTCGAGACATTGAGTTAGAAGAGTTTAAAAAGGCAGCGTCTAAAGCTTCAAAAAATGGAGTGAATCTCAAGCTTGCTGATTTTGCACCACTTTCGCTTTTGAGACCAGATGGGCATCCGGGTCCATACAGGCAATTTCAACCCTTTGCCAAGGACAAAAATGCTAAAGTTCAGACTGATTGTTTGCATTGGTGCTTACCTGGGCCTATAGACTCTTGGAATGACATCATAATGGAGATGGTTGTCAATGGATAA
Protein sequence

>Msa0572500

MRIDSSFNFNHKNYLIKLLLTLFFSVLAFRLLFFQSLPTLTEPQTPPQTSSLSDLQQLPLQNEQEQDQLSPTDTGKCDYFNGDWVPNPLGPIYTNETCDLIESHQNCLKNGRPDRDFLYWKWAPRDCELPIFDPQRFLNLMRGKVWALIGDSISRNHVQSLLCTLSKVEKAVLVYHDEGYKSKSWHFPSYNFSMSVIWSPFLVEAAIFEDINGVSSSEVELYLDKLDSKWTDQYLNFDYIIISTGKWFVKSAIYYENDTILGCHGCSSNKNLTDLGFDFAYRKVLKNVMNFIVSSNHKGLILFRTSTPDHFENGEWFSGGTCNRTEPIKEGEMEIKLLLRILRDIELEEFKKAASKASKNGVNLKLADFAPLSLLRPDGHPGPYRQFQPFAKDKNAKVQTDCLHWCLPGPIDSWNDIIMEMVVNG*