AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0572520

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0572520 MtrunA17_Chr8g0387921 96.487 427 8 2 1 425 1 422 0.0 846
Msa0572520 MtrunA17_Chr8g0347321 60.465 430 155 4 10 425 14 442 0.0 546
Msa0572520 MtrunA17_Chr6g0456691 41.706 422 225 5 8 422 12 419 2.66e-118 352
Msa0572520 MtrunA17_Chr8g0391481 41.129 372 206 9 60 425 11 375 2.96e-100 304
Msa0572520 MtrunA17_Chr5g0414061 42.776 353 189 8 76 422 93 438 4.26e-99 303
Msa0572520 MtrunA17_Chr5g0414041 44.944 356 185 7 73 422 83 433 5.98e-99 302
Msa0572520 MtrunA17_Chr5g0414091 42.330 352 192 6 77 422 85 431 6.88e-99 302
Msa0572520 MtrunA17_Chr3g0140371 42.090 354 192 6 77 425 18 363 1.98e-98 298
Msa0572520 MtrunA17_Chr5g0414051 39.196 398 221 10 32 422 37 420 6.60e-98 299
Msa0572520 MtrunA17_Chr7g0226991 41.972 355 194 7 77 425 57 405 1.61e-96 295
Msa0572520 MtrunA17_Chr2g0283851 42.192 365 191 9 69 423 47 401 1.03e-94 290
Msa0572520 MtrunA17_Chr6g0456681 41.096 365 198 7 66 422 53 408 4.38e-93 286
Msa0572520 MtrunA17_Chr6g0451321 38.120 383 210 9 56 422 86 457 1.40e-87 274
Msa0572520 MtrunA17_Chr1g0188191 35.128 390 235 9 45 422 198 581 4.80e-80 258
Msa0572520 MtrunA17_Chr6g0476041 34.181 354 219 8 77 422 167 514 3.12e-74 241
Msa0572520 MtrunA17_Chr7g0221281 40.417 240 135 5 185 422 3 236 4.17e-62 201
Msa0572520 MtrunA17_Chr1g0175671 32.506 403 217 14 41 418 133 505 2.05e-54 189
Msa0572520 MtrunA17_Chr7g0258851 33.149 362 198 13 76 418 247 583 2.85e-54 190
Msa0572520 MtrunA17_Chr7g0221271 43.655 197 99 3 12 203 16 205 6.89e-53 176
Msa0572520 MtrunA17_Chr5g0414081 41.709 199 109 4 230 422 2 199 1.41e-49 167
Msa0572520 MtrunA17_Chr7g0259711 30.833 360 219 12 77 423 91 433 3.24e-46 165
Msa0572520 MtrunA17_Chr4g0071391 28.256 407 242 15 39 423 69 447 1.03e-45 164
Msa0572520 MtrunA17_Chr5g0413271 30.508 354 210 13 76 418 144 472 8.33e-45 162
Msa0572520 MtrunA17_Chr1g0213281 30.056 356 216 10 77 418 175 511 1.72e-41 154
Msa0572520 MtrunA17_Chr7g0221291 44.937 158 80 1 12 169 21 171 1.83e-41 144
Msa0572520 MtrunA17_Chr3g0113671 28.771 358 233 10 77 424 106 451 4.19e-41 151
Msa0572520 MtrunA17_Chr8g0368461 29.894 378 200 15 76 418 177 524 8.10e-41 152
Msa0572520 MtrunA17_Chr4g0068111 29.508 366 222 14 73 423 110 454 3.97e-40 149
Msa0572520 MtrunA17_Chr7g0269991 29.213 356 209 12 77 418 137 463 7.10e-40 148
Msa0572520 MtrunA17_Chr8g0384421 26.817 399 250 14 41 422 99 472 1.06e-39 148
Msa0572520 MtrunA17_Chr4g0049421 27.424 361 237 9 77 425 48 395 1.16e-39 146
Msa0572520 MtrunA17_Chr8g0384151 29.805 359 209 12 76 422 89 416 4.13e-39 145
Msa0572520 MtrunA17_Chr4g0006991 26.575 365 234 11 73 422 82 427 3.91e-38 142
Msa0572520 MtrunA17_Chr5g0430371 28.378 370 205 10 77 418 58 395 4.77e-38 142
Msa0572520 MtrunA17_Chr8g0360521 28.926 363 209 15 77 418 71 405 8.03e-38 142
Msa0572520 MtrunA17_Chr2g0287901 28.093 388 238 14 55 423 73 438 1.34e-37 142
Msa0572520 MtrunA17_Chr3g0105301 28.986 345 225 8 77 417 43 371 7.54e-37 138
Msa0572520 MtrunA17_Chr3g0085081 29.201 363 215 11 69 424 23 350 1.59e-36 137
Msa0572520 MtrunA17_Chr3g0114101 27.273 352 232 10 77 418 89 426 3.66e-36 137
Msa0572520 MtrunA17_Chr7g0242701 26.667 420 270 13 22 422 27 427 5.50e-36 137
Msa0572520 MtrunA17_Chr4g0005491 26.121 379 236 16 66 422 51 407 6.88e-36 136
Msa0572520 MtrunA17_Chr3g0091031 28.375 363 218 11 69 424 23 350 1.96e-35 134
Msa0572520 MtrunA17_Chr1g0171171 25.532 423 248 15 19 422 1 375 2.18e-35 134
Msa0572520 MtrunA17_Chr3g0085071 28.255 361 217 10 69 422 38 363 6.49e-35 132
Msa0572520 MtrunA17_Chr7g0243301 27.123 365 238 14 74 424 61 411 5.41e-34 131
Msa0572520 MtrunA17_Chr1g0210771 27.066 351 218 13 76 418 58 378 9.07e-34 130
Msa0572520 MtrunA17_Chr6g0455901 26.087 437 258 15 17 422 14 416 9.79e-34 130
Msa0572520 MtrunA17_Chr5g0437601 26.761 355 230 12 77 418 83 420 1.42e-33 130
Msa0572520 MtrunA17_Chr5g0437291 27.151 372 237 13 69 424 160 513 1.71e-32 128
Msa0572520 MtrunA17_Chr4g0070341 25.341 367 236 12 72 422 127 471 2.10e-32 127
Msa0572520 MtrunA17_Chr3g0097891 29.745 353 210 12 77 423 31 351 5.57e-31 121
Msa0572520 MtrunA17_Chr2g0314801 29.775 356 207 13 76 422 32 353 1.24e-30 120
Msa0572520 MtrunA17_Chr3g0126021 25.745 369 215 15 77 423 129 460 1.45e-28 117
Msa0572520 MtrunA17_Chr4g0045941 26.346 353 226 9 74 423 30 351 1.48e-28 115
Msa0572520 MtrunA17_Chr2g0283581 28.249 354 213 13 71 418 36 354 1.51e-28 115
Msa0572520 MtrunA17_Chr2g0325481 27.350 351 214 13 74 418 47 362 3.33e-28 114
Msa0572520 MtrunA17_Chr4g0045951 25.000 380 247 10 51 423 15 363 1.16e-26 110
Msa0572520 MtrunA17_Chr5g0413851 26.989 352 219 12 77 423 42 360 1.40e-26 109
Msa0572520 MtrunA17_Chr2g0314791 26.124 356 216 10 77 423 29 346 5.26e-25 105
Msa0572520 MtrunA17_Chr4g0005481 24.932 365 229 12 77 422 108 446 1.86e-24 104
Msa0572520 MtrunA17_Chr4g0041011 25.852 352 218 10 74 418 44 359 1.61e-23 101
Msa0572520 MtrunA17_Chr7g0275501 65.278 72 20 2 350 421 2 68 1.87e-23 93.2
Msa0572520 MtrunA17_Chr1g0209921 29.583 240 148 7 76 310 43 266 1.31e-18 85.5
Msa0572520 MtrunA17_Chr1g0178081 26.172 256 170 8 26 270 8 255 1.35e-15 77.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0572520 AT4G11090.1 55.734 436 172 4 1 425 3 428 0.0 517
Msa0572520 AT4G23790.1 57.611 427 173 6 5 425 6 430 1.16e-177 503
Msa0572520 AT1G01430.1 53.211 436 186 9 5 424 16 449 3.67e-161 462
Msa0572520 AT4G01080.1 52.099 405 167 5 22 424 53 432 3.60e-155 446
Msa0572520 AT5G15890.1 46.724 351 175 6 73 422 183 522 1.91e-109 332
Msa0572520 AT5G15900.1 40.047 427 233 8 1 422 1 409 3.98e-105 318
Msa0572520 AT5G15900.2 40.047 427 233 8 1 422 20 428 7.53e-105 318
Msa0572520 AT3G02440.1 40.051 392 210 7 47 424 90 470 8.07e-103 314
Msa0572520 AT3G02440.2 40.051 392 210 7 47 424 98 478 1.04e-102 314
Msa0572520 AT1G70230.1 38.118 425 246 9 1 422 1 411 2.19e-102 311
Msa0572520 AT3G28150.1 37.097 372 219 6 57 422 48 410 4.47e-88 274
Msa0572520 AT5G51640.1 34.840 376 220 9 60 422 125 488 4.98e-78 251
Msa0572520 AT4G25360.1 33.586 396 240 10 41 422 138 524 2.60e-74 242
Msa0572520 AT4G25360.2 33.586 396 240 10 41 422 138 524 2.60e-74 242
Msa0572520 AT5G06700.1 31.622 370 202 11 77 423 255 596 8.51e-51 181
Msa0572520 AT3G12060.1 31.806 371 200 13 77 423 196 537 1.15e-50 179
Msa0572520 AT3G06080.2 31.868 364 211 13 77 423 103 446 3.06e-46 166
Msa0572520 AT5G49340.1 31.180 356 217 9 75 418 98 437 4.77e-46 165
Msa0572520 AT5G19160.1 31.492 362 218 12 77 423 99 445 2.62e-44 160
Msa0572520 AT5G20590.1 30.387 362 205 14 77 418 141 475 9.67e-44 159
Msa0572520 AT1G60790.1 31.608 367 198 15 77 418 190 528 1.67e-42 157
Msa0572520 AT2G14530.1 27.778 378 225 13 68 422 54 406 5.77e-40 148
Msa0572520 AT1G48880.1 29.805 359 201 12 76 419 114 436 1.21e-38 145
Msa0572520 AT5G06230.2 28.877 374 226 13 70 424 14 366 1.56e-38 143
Msa0572520 AT5G64020.1 28.000 375 218 11 77 422 57 408 2.12e-38 143
Msa0572520 AT5G64470.2 29.640 361 226 13 77 423 55 401 2.57e-38 143
Msa0572520 AT5G06230.1 28.877 374 226 13 70 424 55 407 3.11e-38 143
Msa0572520 AT5G64470.3 29.888 358 223 13 77 423 55 395 3.92e-38 142
Msa0572520 AT5G20680.6 28.418 373 207 15 77 422 118 457 4.17e-38 144
Msa0572520 AT2G40160.1 28.010 382 244 12 59 423 57 424 4.63e-38 143
Msa0572520 AT2G40160.2 28.382 377 239 12 59 418 57 419 5.80e-38 143
Msa0572520 AT5G20680.4 28.418 373 207 15 77 422 212 551 8.26e-38 144
Msa0572520 AT5G20680.1 28.418 373 207 15 77 422 212 551 8.26e-38 144
Msa0572520 AT5G20680.3 28.418 373 207 15 77 422 212 551 8.26e-38 144
Msa0572520 AT5G20680.5 28.418 373 207 15 77 422 212 551 8.26e-38 144
Msa0572520 AT3G62390.1 25.888 394 234 15 48 418 109 467 8.52e-38 143
Msa0572520 AT5G20680.2 28.418 373 207 15 77 422 194 533 1.08e-37 144
Msa0572520 AT5G58600.1 28.371 356 226 9 77 423 66 401 1.34e-37 141
Msa0572520 AT1G73140.1 27.441 379 214 15 65 418 59 401 1.72e-35 135
Msa0572520 AT3G11570.1 28.655 342 214 12 93 423 98 420 3.11e-35 135
Msa0572520 AT5G01360.2 26.230 366 229 15 73 419 32 375 6.14e-35 133
Msa0572520 AT5G01360.1 26.230 366 229 15 73 419 88 431 2.09e-34 133
Msa0572520 AT2G40320.1 25.412 425 268 15 14 418 23 418 2.16e-34 133
Msa0572520 AT2G30010.1 25.131 382 245 10 60 423 39 397 2.24e-34 132
Msa0572520 AT2G30010.2 25.326 383 243 11 60 423 82 440 3.78e-34 132
Msa0572520 AT3G54260.1 27.322 366 208 15 77 424 51 376 1.71e-33 129
Msa0572520 AT3G11030.1 26.857 350 235 10 77 417 106 443 2.10e-33 130
Msa0572520 AT2G30900.1 28.691 359 199 13 77 418 43 361 1.08e-32 127
Msa0572520 AT3G55990.1 23.773 387 270 11 48 424 114 485 1.19e-32 129
Msa0572520 AT2G40150.1 24.722 360 253 9 76 424 70 422 2.02e-31 124
Msa0572520 AT2G31110.2 28.647 377 225 12 50 418 18 358 2.44e-31 123
Msa0572520 AT2G38320.1 25.648 386 251 14 58 424 38 406 4.55e-31 123
Msa0572520 AT3G14850.2 29.014 355 211 14 77 423 34 355 3.81e-30 120
Msa0572520 AT2G34070.1 27.089 347 222 9 74 418 62 379 1.02e-29 119
Msa0572520 AT5G01620.3 26.630 368 222 15 76 422 117 457 1.98e-29 119
Msa0572520 AT3G06080.1 32.283 254 150 8 77 318 103 346 1.98e-29 117
Msa0572520 AT5G01620.2 26.630 368 222 15 76 422 109 449 2.05e-29 119
Msa0572520 AT5G01620.1 26.630 368 222 15 76 422 109 449 2.05e-29 119
Msa0572520 AT5G19160.2 32.927 246 145 8 77 311 99 335 2.06e-29 117
Msa0572520 AT1G29050.1 27.841 352 216 10 77 423 61 379 8.93e-29 116
Msa0572520 AT1G78710.1 26.158 367 208 15 76 424 37 358 4.29e-27 111
Msa0572520 AT2G42570.1 26.446 363 218 14 62 415 35 357 1.17e-26 110
Msa0572520 AT5G64470.4 29.104 268 172 10 77 333 55 315 1.17e-25 106
Msa0572520 AT5G64470.1 28.996 269 173 10 77 334 55 316 1.24e-25 106
Msa0572520 AT2G40160.3 28.409 264 171 7 59 312 57 312 6.56e-25 104
Msa0572520 AT2G38320.2 24.850 334 218 11 107 424 7 323 9.21e-25 104
Msa0572520 AT3G62390.2 25.256 293 189 9 48 325 109 386 2.32e-24 104
Msa0572520 AT2G37720.1 24.093 386 237 11 62 422 128 482 3.30e-24 105
Msa0572520 AT5G20590.2 28.458 253 149 9 77 312 141 378 4.42e-24 103
Msa0572520 AT5G58600.2 28.270 237 154 6 77 310 66 289 6.10e-23 98.6
Msa0572520 AT5G06230.3 28.517 263 162 9 70 315 55 308 1.06e-22 98.2
Msa0572520 AT2G31110.3 30.049 203 133 3 50 252 18 211 5.49e-21 92.4
Msa0572520 AT2G30900.2 30.806 211 130 6 77 285 43 239 2.52e-20 90.9
Msa0572520 AT1G78710.2 29.050 179 114 4 76 252 30 197 1.42e-16 80.5
Msa0572520 AT3G14850.1 26.855 283 166 14 149 423 3 252 1.94e-11 64.3

Find 91 sgRNAs with CRISPR-Local

Find 195 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAGTTTGAGGTGGGGTTT+GGG 0.248412 4_1:-75421650 None:intergenic
CATGTTTCATTTGTATAAAT+TGG 0.250767 4_1:-75421938 None:intergenic
ACTTGATGCGTGGTAAAGTA+TGG 0.254267 4_1:+75422083 Msa0572520:CDS
AAGAAGTTTGAGGTGGGGTT+TGG 0.275870 4_1:-75421651 None:intergenic
TCGACAAACTGGATAGTAAA+TGG 0.284885 4_1:+75423732 Msa0572520:CDS
CTATGAGAATGACACTATAT+TGG 0.295402 4_1:+75423826 Msa0572520:CDS
ACTTCAGTATGTCAGTTATT+TGG 0.298802 4_1:+75423636 Msa0572520:CDS
GGATAGATTGAGAATGGTTT+TGG 0.300507 4_1:-75421408 None:intergenic
CTTGCTCTGCACGCTTTCTA+AGG 0.308263 4_1:+75422147 Msa0572520:CDS
TGAAGGAAAGTGCCAGCTTT+TGG 0.308991 4_1:-75423609 None:intergenic
TGTTTGCATTGGTGCTTACC+TGG 0.316596 4_1:+75424268 Msa0572520:CDS
TCCATTTCACCTTCTTTAAT+CGG 0.319502 4_1:-75424043 None:intergenic
GGATAACAAAATCAGTTAAA+TGG 0.327061 4_1:+75424337 Msa0572520:exon
TGAGTTAGAAGAGTTTAAAA+AGG 0.345867 4_1:+75424099 Msa0572520:CDS
AACAAGAACCTGACAGATCT+AGG 0.351818 4_1:+75423869 Msa0572520:CDS
CTGAACATGGTTACGAGAAA+TGG 0.358145 4_1:-75422122 None:intergenic
TAAATTGGACCCAATGGGTT+TGG 0.401463 4_1:-75421923 None:intergenic
TATGAGAATGACACTATATT+GGG 0.402812 4_1:+75423827 Msa0572520:CDS
CAAAATCAAGCCCTTGTGAT+TGG 0.411626 4_1:-75423948 None:intergenic
TTTGGAGTTGGTGTTGCTTA+CGG 0.415872 4_1:-75421429 None:intergenic
TATGGACCCGGATGCCCATC+TGG 0.419716 4_1:-75424193 None:intergenic
TTTGAGACCAGATGGGCATC+CGG 0.423399 4_1:+75424186 Msa0572520:CDS
GGTTTGGGGTTCAGTGAGAG+TGG 0.428680 4_1:-75421635 None:intergenic
AAGGGTTGAAATTGCCTGTA+TGG 0.436352 4_1:-75424211 None:intergenic
TGAGCTATATCTCGACAAAC+TGG 0.441451 4_1:+75423721 Msa0572520:CDS
ACCGATTAAAGAAGGTGAAA+TGG 0.442228 4_1:+75424042 Msa0572520:CDS
CTCTTGGAATGACATCATAA+TGG 0.463666 4_1:+75424300 Msa0572520:CDS
GTTTGGGGTTCAGTGAGAGT+GGG 0.464045 4_1:-75421634 None:intergenic
TTTGAAGAGGGAGTTGTTGA+AGG 0.465492 4_1:-75421684 None:intergenic
TTTGTATAAATTGGACCCAA+TGG 0.465556 4_1:-75421929 None:intergenic
ATGTCATTCCAAGAGTCTAT+AGG 0.471038 4_1:-75424292 None:intergenic
TTGTATAAATTGGACCCAAT+GGG 0.480307 4_1:-75421928 None:intergenic
AAAGAGAAGAAGTTTGAGGT+GGG 0.480555 4_1:-75421657 None:intergenic
GATTACATTATTATTTCAAC+GGG 0.486220 4_1:+75423776 Msa0572520:CDS
GTTTGCATTGGTGCTTACCT+GGG 0.490210 4_1:+75424269 Msa0572520:CDS
CGATTACATTATTATTTCAA+CGG 0.495469 4_1:+75423775 Msa0572520:CDS
TACCTGGGCCTATAGACTCT+TGG 0.497198 4_1:+75424284 Msa0572520:CDS
TCAGAAAGAGAAGAAGTTTG+AGG 0.498283 4_1:-75421661 None:intergenic
TTGAGACCAGATGGGCATCC+GGG 0.502873 4_1:+75424187 Msa0572520:CDS
GCTTACGGATAGATTGAGAA+TGG 0.505838 4_1:-75421414 None:intergenic
ATGGGCATCCGGGTCCATAC+AGG 0.516143 4_1:+75424197 Msa0572520:CDS
GAAGTTTGAGGTGGGGTTTG+GGG 0.523244 4_1:-75421649 None:intergenic
TTATTATTTCAACGGGAAAG+TGG 0.527276 4_1:+75423783 Msa0572520:CDS
ATCAAGTTGAGAAACCTTTG+AGG 0.527808 4_1:-75422067 None:intergenic
ATAGGTAACTCACAATCTCT+AGG 0.532576 4_1:-75422037 None:intergenic
GATTGGGTACCAAACCCATT+GGG 0.533030 4_1:+75421914 Msa0572520:CDS
GACACTATATTGGGCTGTCA+CGG 0.534615 4_1:+75423836 Msa0572520:CDS
TTCAGACTGATTGTTTGCAT+TGG 0.536161 4_1:+75424257 Msa0572520:CDS
TAGCTAGAAATTACACAACT+TGG 0.538439 4_1:+75421335 None:intergenic
TTCCAAGAGTCTATAGGCCC+AGG 0.538902 4_1:-75424286 None:intergenic
GACATACTGAAGTTGTATGA+AGG 0.541369 4_1:-75423626 None:intergenic
AAGAGAAGAAGTTTGAGGTG+GGG 0.541698 4_1:-75421656 None:intergenic
GAAAATGGAGAGTGGTTTAG+CGG 0.544102 4_1:+75423998 Msa0572520:CDS
TCTGGTCTCAAAAGCGAAAG+TGG 0.551364 4_1:-75424175 None:intergenic
CAACTCTCTCCCACCGACAC+TGG 0.551711 4_1:+75421724 Msa0572520:CDS
CATAGTCTCTTCCAATCACA+AGG 0.551938 4_1:+75423937 Msa0572520:CDS
GAAAGAGAAGAAGTTTGAGG+TGG 0.556991 4_1:-75421658 None:intergenic
TGATTGGGTACCAAACCCAT+TGG 0.557892 4_1:+75421913 Msa0572520:CDS
AGTCAAACCCTAGATCTGTC+AGG 0.559564 4_1:-75423877 None:intergenic
GGTAAAGTATGGGCACTGAT+TGG 0.559712 4_1:+75422094 Msa0572520:CDS
GAGTGAGAAGTAACTTGATG+AGG 0.562325 4_1:-75421564 None:intergenic
ATAATGGAGATGGTTGTCAA+TGG 0.563525 4_1:+75424316 Msa0572520:CDS
CTTAGAAAGCGTGCAGAGCA+AGG 0.565769 4_1:-75422146 None:intergenic
AGAACTGAACCGATTAAAGA+AGG 0.566208 4_1:+75424034 Msa0572520:CDS
CTTGATGCGTGGTAAAGTAT+GGG 0.577745 4_1:+75422084 Msa0572520:CDS
ATAGCAGCTTCCACAAGGAA+TGG 0.582566 4_1:-75423662 None:intergenic
GCAATTTCAACCCTTTGCCA+AGG 0.584888 4_1:+75424219 Msa0572520:CDS
ACAAAGAAGAAGAAATAATG+AGG 0.585396 4_1:+75421506 Msa0572520:exon
CCTCATGCTAGTTGTGCATG+TGG 0.592160 4_1:+75424381 Msa0572520:three_prime_UTR
AGGGATACAAGTCCAAAAGC+TGG 0.595283 4_1:+75423597 Msa0572520:CDS
GTCTTAGTCTACCACGATGA+GGG 0.597331 4_1:+75423578 Msa0572520:CDS
ACAAGAACCTGACAGATCTA+GGG 0.597345 4_1:+75423870 Msa0572520:CDS
ATGTTACTGACCAGTGTCGG+TGG 0.597544 4_1:-75421734 None:intergenic
CTCATGCTAGTTGTGCATGT+GGG 0.605238 4_1:+75424382 Msa0572520:three_prime_UTR
TGTTACTGTTTCAACAAACA+CGG 0.607055 4_1:-75421466 None:intergenic
TGCAGAGCAAGGACTGAACA+TGG 0.608168 4_1:-75422135 None:intergenic
TATTTGGTCACCATTCCTTG+TGG 0.611002 4_1:+75423652 Msa0572520:CDS
GAATGACATCATAATGGAGA+TGG 0.612407 4_1:+75424306 Msa0572520:CDS
TGAAATTGCCTGTATGGACC+CGG 0.620052 4_1:-75424205 None:intergenic
AGGTTTCTCAACTTGATGCG+TGG 0.635087 4_1:+75422073 Msa0572520:CDS
AGGGAAGAAAACTAACTGCA+AGG 0.642801 4_1:-75424361 None:intergenic
CCACATGCACAACTAGCATG+AGG 0.651752 4_1:-75424381 None:intergenic
TGTTACTGACCAGTGTCGGT+GGG 0.657177 4_1:-75421733 None:intergenic
CACATGCACAACTAGCATGA+GGG 0.658468 4_1:-75424380 None:intergenic
TGTCTTAGTCTACCACGATG+AGG 0.661682 4_1:+75423577 Msa0572520:CDS
AATGGAGAGTGGTTTAGCGG+CGG 0.675176 4_1:+75424001 Msa0572520:CDS
TGTGTAGAGTTGGAAATGCA+TGG 0.692938 4_1:+75424526 Msa0572520:three_prime_UTR
CTTGGTTCTGATCTATGCTG+TGG 0.703009 4_1:+75421353 Msa0572520:five_prime_UTR
ATAGTCTCTTCCAATCACAA+GGG 0.713429 4_1:+75423938 Msa0572520:CDS
TGGACTTGTATCCCTCATCG+TGG 0.719158 4_1:-75423589 None:intergenic
AGAATGTTACTGACCAGTGT+CGG 0.728713 4_1:-75421737 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GATTTTTACATTTTTTTTTT+TGG + chr4_1:75421862-75421881 Msa0572520:intron 10.0%
!!! ATTTTATTTACAATATTTGA+TGG + chr4_1:75422754-75422773 Msa0572520:intron 10.0%
!!! TATAGTTTTATATTTCATTT+TGG + chr4_1:75424503-75424522 Msa0572520:three_prime_UTR 10.0%
!! ATTTAGATAAAAAATAGTGA+AGG - chr4_1:75423247-75423266 None:intergenic 15.0%
!!! TCTTCTATTTAAAACAAAAT+CGG - chr4_1:75423340-75423359 None:intergenic 15.0%
!!! AATCATATAAACTTTTACAA+TGG + chr4_1:75423505-75423524 Msa0572520:intron 15.0%
!!! GGTAAATGTGATTATTTTAA+TGG + chr4_1:75421890-75421909 Msa0572520:intron 20.0%
!!! TGATTATTTTAATGGTGATT+GGG + chr4_1:75421898-75421917 Msa0572520:CDS 20.0%
!! CATGTTTCATTTGTATAAAT+TGG - chr4_1:75421941-75421960 None:intergenic 20.0%
!! ATCAAAATTGCTTGAAAAAT+GGG + chr4_1:75421978-75421997 Msa0572520:CDS 20.0%
!!! ATATTGTTTTGAACTTTGAT+TGG + chr4_1:75422788-75422807 Msa0572520:intron 20.0%
!!! GTCTATACTTTTTCATTTTT+TGG - chr4_1:75422887-75422906 None:intergenic 20.0%
!!! AAAAATGAAAAAGTATAGAC+TGG + chr4_1:75422887-75422906 Msa0572520:intron 20.0%
!! TCAATTAAGAAGAGTTAAAA+AGG + chr4_1:75422932-75422951 Msa0572520:intron 20.0%
!! AAAAGGATAATGATTATGAA+AGG + chr4_1:75422949-75422968 Msa0572520:intron 20.0%
!! ATAATGATTATGAAAGGTAA+TGG + chr4_1:75422955-75422974 Msa0572520:intron 20.0%
!! TCAATTAAGAAGAGTTAAAA+AGG + chr4_1:75422932-75422951 Msa0572520:intron 20.0%
!! CGATTACATTATTATTTCAA+CGG + chr4_1:75423775-75423794 Msa0572520:CDS 20.0%
!! GATTACATTATTATTTCAAC+GGG + chr4_1:75423776-75423795 Msa0572520:CDS 20.0%
! ACAAAGAAGAAGAAATAATG+AGG + chr4_1:75421506-75421525 Msa0572520:exon 25.0%
!! ACTTGATGAGGTAATTTTTA+TGG - chr4_1:75421555-75421574 None:intergenic 25.0%
!!! ACATTTTTTTTTTTGGATGC+AGG + chr4_1:75421869-75421888 Msa0572520:intron 25.0%
!!! GTGATTATTTTAATGGTGAT+TGG + chr4_1:75421897-75421916 Msa0572520:CDS 25.0%
!!! AATTTTGATGTGATTCAATG+AGG - chr4_1:75421967-75421986 None:intergenic 25.0%
! CATCAAAATTGCTTGAAAAA+TGG + chr4_1:75421977-75421996 Msa0572520:CDS 25.0%
!!! GTCTTTTTTACGTGTTTTTT+CGG + chr4_1:75422318-75422337 Msa0572520:intron 25.0%
! CATATAAGCTGTAATAGAAT+AGG + chr4_1:75422474-75422493 Msa0572520:intron 25.0%
! ATGAAGTAATTAATCAACCT+AGG + chr4_1:75422527-75422546 Msa0572520:intron 25.0%
! AAAAGGATAATGATCATGAA+AGG + chr4_1:75423055-75423074 Msa0572520:intron 25.0%
! ATAATGATCATGAAAGGTAA+TGG + chr4_1:75423061-75423080 Msa0572520:intron 25.0%
!!! GCTATTTTTGAAGACATAAA+CGG + chr4_1:75423680-75423699 Msa0572520:CDS 25.0%
! TATGAGAATGACACTATATT+GGG + chr4_1:75423827-75423846 Msa0572520:CDS 25.0%
! TGAGTTAGAAGAGTTTAAAA+AGG + chr4_1:75424099-75424118 Msa0572520:CDS 25.0%
! GGATAACAAAATCAGTTAAA+TGG + chr4_1:75424337-75424356 Msa0572520:exon 25.0%
TGTTACTGTTTCAACAAACA+CGG - chr4_1:75421469-75421488 None:intergenic 30.0%
!! GTTCTTGTTCATTTTGAAGA+GGG - chr4_1:75421699-75421718 None:intergenic 30.0%
!! TGTTCTTGTTCATTTTGAAG+AGG - chr4_1:75421700-75421719 None:intergenic 30.0%
GAAATAGTGTTTAATTCCTG+TGG + chr4_1:75421782-75421801 Msa0572520:intron 30.0%
TTTCCAAAACAAGTTAACGT+TGG + chr4_1:75421839-75421858 Msa0572520:intron 30.0%
TTCCAAAACAAGTTAACGTT+GGG + chr4_1:75421840-75421859 Msa0572520:intron 30.0%
TTGTATAAATTGGACCCAAT+GGG - chr4_1:75421931-75421950 None:intergenic 30.0%
TTTGTATAAATTGGACCCAA+TGG - chr4_1:75421932-75421951 None:intergenic 30.0%
!!! AGATTTTTTGTACTGGAAGT+GGG + chr4_1:75422012-75422031 Msa0572520:CDS 30.0%
!!! TACCTATTTTTGATCCTCAA+AGG + chr4_1:75422053-75422072 Msa0572520:CDS 30.0%
! AACCTTTGAGGATCAAAAAT+AGG - chr4_1:75422058-75422077 None:intergenic 30.0%
CTCAAAGAAACATTCACAAA+AGG - chr4_1:75422286-75422305 None:intergenic 30.0%
! CTTTTGTGAATGTTTCTTTG+AGG + chr4_1:75422284-75422303 Msa0572520:intron 30.0%
AGAATAACTCAAGAATCTGT+TGG + chr4_1:75422409-75422428 Msa0572520:intron 30.0%
CTAGCTCAATAGATAAGATA+AGG + chr4_1:75422621-75422640 Msa0572520:intron 30.0%
! CTTCTTTCCTCAATTACTTT+TGG - chr4_1:75422733-75422752 None:intergenic 30.0%
AAATGCATAATTGACTAGAC+CGG - chr4_1:75423135-75423154 None:intergenic 30.0%
TGTACATGAAGAATAAACGA+GGG - chr4_1:75423209-75423228 None:intergenic 30.0%
! ATTTAAAACAAAATCGGCGA+AGG - chr4_1:75423334-75423353 None:intergenic 30.0%
! TTTGTCAATTTTGTGAAACC+TGG + chr4_1:75423430-75423449 Msa0572520:intron 30.0%
ACTTCAGTATGTCAGTTATT+TGG + chr4_1:75423636-75423655 Msa0572520:CDS 30.0%
TTATTATTTCAACGGGAAAG+TGG + chr4_1:75423783-75423802 Msa0572520:CDS 30.0%
CTATGAGAATGACACTATAT+TGG + chr4_1:75423826-75423845 Msa0572520:CDS 30.0%
TCCATTTCACCTTCTTTAAT+CGG - chr4_1:75424046-75424065 None:intergenic 30.0%
! TGGAAATTAAGCTTTTGCTT+AGG + chr4_1:75424062-75424081 Msa0572520:CDS 30.0%
! AACTTTAGCATTTTTGTCCT+TGG - chr4_1:75424239-75424258 None:intergenic 30.0%
!!! ATTGAGAATGGTTTTGGTTG+GGG - chr4_1:75421405-75421424 None:intergenic 35.0%
!!! GATTGAGAATGGTTTTGGTT+GGG - chr4_1:75421406-75421425 None:intergenic 35.0%
!!! AGATTGAGAATGGTTTTGGT+TGG - chr4_1:75421407-75421426 None:intergenic 35.0%
! GGATAGATTGAGAATGGTTT+TGG - chr4_1:75421411-75421430 None:intergenic 35.0%
CAACACCAACTCCAAAAAAT+CGG + chr4_1:75421436-75421455 Msa0572520:five_prime_UTR 35.0%
! AAAGAGAAGAAGTTTGAGGT+GGG - chr4_1:75421660-75421679 None:intergenic 35.0%
TCAGAAAGAGAAGAAGTTTG+AGG - chr4_1:75421664-75421683 None:intergenic 35.0%
TAATTGAACATTGAGACCAC+AGG - chr4_1:75421801-75421820 None:intergenic 35.0%
GGTCTCAATGTTCAATTAGT+CGG + chr4_1:75421803-75421822 Msa0572520:intron 35.0%
!!! ATCCCAACGTTAACTTGTTT+TGG - chr4_1:75421845-75421864 None:intergenic 35.0%
CAGTACAAAAAATCTCTGTC+TGG - chr4_1:75422007-75422026 None:intergenic 35.0%
!!! CAGACAGAGATTTTTTGTAC+TGG + chr4_1:75422005-75422024 Msa0572520:CDS 35.0%
!!! GAGATTTTTTGTACTGGAAG+TGG + chr4_1:75422011-75422030 Msa0572520:CDS 35.0%
ATAGGTAACTCACAATCTCT+AGG - chr4_1:75422040-75422059 None:intergenic 35.0%
!! ATCAAGTTGAGAAACCTTTG+AGG - chr4_1:75422070-75422089 None:intergenic 35.0%
GACAACAACGATGAATACAA+TGG - chr4_1:75422203-75422222 None:intergenic 35.0%
CATCGTTGTTGTCAATAGAT+TGG + chr4_1:75422210-75422229 Msa0572520:intron 35.0%
CACAAAAGGTCAGACAAAAA+AGG - chr4_1:75422272-75422291 None:intergenic 35.0%
AAACTACCAAAGTTATGCGT+TGG - chr4_1:75422374-75422393 None:intergenic 35.0%
GGTAGTTTATTTATCGAGTC+TGG + chr4_1:75422386-75422405 Msa0572520:intron 35.0%
ATAGAATAGGTCTATGCTCT+TGG + chr4_1:75422487-75422506 Msa0572520:intron 35.0%
AGAATATAGCACCTGAGAAT+CGG + chr4_1:75422664-75422683 Msa0572520:intron 35.0%
ATCAGCTCCAAAAGTAATTG+AGG + chr4_1:75422723-75422742 Msa0572520:intron 35.0%
!!! TTTTCAAGTTTTGCCAGTCT+AGG - chr4_1:75422903-75422922 None:intergenic 35.0%
GTGTACATGAAGAATAAACG+AGG - chr4_1:75423210-75423229 None:intergenic 35.0%
TTCAATTCAGTCCAAACAAG+TGG - chr4_1:75423465-75423484 None:intergenic 35.0%
!!! CTTGAACTATTTTGACCTTC+AGG + chr4_1:75423544-75423563 Msa0572520:intron 35.0%
GACATACTGAAGTTGTATGA+AGG - chr4_1:75423629-75423648 None:intergenic 35.0%
TCGACAAACTGGATAGTAAA+TGG + chr4_1:75423732-75423751 Msa0572520:CDS 35.0%
ATAGTCTCTTCCAATCACAA+GGG + chr4_1:75423938-75423957 Msa0572520:CDS 35.0%
! ATTTTCAAAATGGTCAGGTG+TGG - chr4_1:75423984-75424003 None:intergenic 35.0%
!! ACACCTGACCATTTTGAAAA+TGG + chr4_1:75423983-75424002 Msa0572520:CDS 35.0%
! TCTCCATTTTCAAAATGGTC+AGG - chr4_1:75423989-75424008 None:intergenic 35.0%
!! ACCATTTTGAAAATGGAGAG+TGG + chr4_1:75423990-75424009 Msa0572520:CDS 35.0%
! ACCACTCTCCATTTTCAAAA+TGG - chr4_1:75423994-75424013 None:intergenic 35.0%
AGAACTGAACCGATTAAAGA+AGG + chr4_1:75424034-75424053 Msa0572520:CDS 35.0%
ACCGATTAAAGAAGGTGAAA+TGG + chr4_1:75424042-75424061 Msa0572520:CDS 35.0%
! GCGTCTAAAGCTTCAAAAAA+TGG + chr4_1:75424124-75424143 Msa0572520:CDS 35.0%
TTCAGACTGATTGTTTGCAT+TGG + chr4_1:75424257-75424276 Msa0572520:CDS 35.0%
ATGTCATTCCAAGAGTCTAT+AGG - chr4_1:75424295-75424314 None:intergenic 35.0%
CTCTTGGAATGACATCATAA+TGG + chr4_1:75424300-75424319 Msa0572520:CDS 35.0%
! GAATGACATCATAATGGAGA+TGG + chr4_1:75424306-75424325 Msa0572520:CDS 35.0%
! ATAATGGAGATGGTTGTCAA+TGG + chr4_1:75424316-75424335 Msa0572520:CDS 35.0%
!! TTCATTTTGGTGTGTAGAGT+TGG + chr4_1:75424516-75424535 Msa0572520:three_prime_UTR 35.0%
GCTTACGGATAGATTGAGAA+TGG - chr4_1:75421417-75421436 None:intergenic 40.0%
!! TTTGGAGTTGGTGTTGCTTA+CGG - chr4_1:75421432-75421451 None:intergenic 40.0%
!!! AGACACCGATTTTTTGGAGT+TGG - chr4_1:75421444-75421463 None:intergenic 40.0%
!!! ACGGAAAGACACCGATTTTT+TGG - chr4_1:75421450-75421469 None:intergenic 40.0%
GAGTGAGAAGTAACTTGATG+AGG - chr4_1:75421567-75421586 None:intergenic 40.0%
! AAGAGAAGAAGTTTGAGGTG+GGG - chr4_1:75421659-75421678 None:intergenic 40.0%
GAAAGAGAAGAAGTTTGAGG+TGG - chr4_1:75421661-75421680 None:intergenic 40.0%
TTTGAAGAGGGAGTTGTTGA+AGG - chr4_1:75421687-75421706 None:intergenic 40.0%
AGAATGTTACTGACCAGTGT+CGG - chr4_1:75421740-75421759 None:intergenic 40.0%
! TAAATTGGACCCAATGGGTT+TGG - chr4_1:75421926-75421945 None:intergenic 40.0%
ACTTGATGCGTGGTAAAGTA+TGG + chr4_1:75422083-75422102 Msa0572520:CDS 40.0%
CTTGATGCGTGGTAAAGTAT+GGG + chr4_1:75422084-75422103 Msa0572520:CDS 40.0%
CTGAACATGGTTACGAGAAA+TGG - chr4_1:75422125-75422144 None:intergenic 40.0%
GTTGGAACCAAAACAAGATC+CGG - chr4_1:75422356-75422375 None:intergenic 40.0%
TTGGTTCCAACGCATAACTT+TGG + chr4_1:75422365-75422384 Msa0572520:intron 40.0%
TCAACCTAGGATGAAAAGAG+TGG + chr4_1:75422540-75422559 Msa0572520:intron 40.0%
! TTCTCCACTCTTTTCATCCT+AGG - chr4_1:75422547-75422566 None:intergenic 40.0%
!!! ACAACAACTCTGGGATTTTG+AGG + chr4_1:75422692-75422711 Msa0572520:intron 40.0%
TACGTGTATGGACTTGTAAC+TGG - chr4_1:75423103-75423122 None:intergenic 40.0%
AACAAGTGGTCAAAAAGTCC+AGG - chr4_1:75423451-75423470 None:intergenic 40.0%
! GGACTTTTTGACCACTTGTT+TGG + chr4_1:75423451-75423470 Msa0572520:intron 40.0%
GTTTGGACTGAATTGAAGCA+TGG + chr4_1:75423468-75423487 Msa0572520:intron 40.0%
!! AGACAGCTTTTTCTACCTGA+AGG - chr4_1:75423562-75423581 None:intergenic 40.0%
TATTTGGTCACCATTCCTTG+TGG + chr4_1:75423652-75423671 Msa0572520:CDS 40.0%
CAAAAATAGCAGCTTCCACA+AGG - chr4_1:75423670-75423689 None:intergenic 40.0%
TGAGCTATATCTCGACAAAC+TGG + chr4_1:75423721-75423740 Msa0572520:CDS 40.0%
AACAAGAACCTGACAGATCT+AGG + chr4_1:75423869-75423888 Msa0572520:CDS 40.0%
ACAAGAACCTGACAGATCTA+GGG + chr4_1:75423870-75423889 Msa0572520:CDS 40.0%
CATAGTCTCTTCCAATCACA+AGG + chr4_1:75423937-75423956 Msa0572520:CDS 40.0%
CAAAATCAAGCCCTTGTGAT+TGG - chr4_1:75423951-75423970 None:intergenic 40.0%
! ACAAGGGCTTGATTTTGTTC+AGG + chr4_1:75423954-75423973 Msa0572520:CDS 40.0%
GAAAATGGAGAGTGGTTTAG+CGG + chr4_1:75423998-75424017 Msa0572520:CDS 40.0%
!!! TTTCGCTTTTGAGACCAGAT+GGG + chr4_1:75424179-75424198 Msa0572520:CDS 40.0%
AAGGGTTGAAATTGCCTGTA+TGG - chr4_1:75424214-75424233 None:intergenic 40.0%
! GCATTTTTGTCCTTGGCAAA+GGG - chr4_1:75424232-75424251 None:intergenic 40.0%
! AGCATTTTTGTCCTTGGCAA+AGG - chr4_1:75424233-75424252 None:intergenic 40.0%
AGGGAAGAAAACTAACTGCA+AGG - chr4_1:75424364-75424383 None:intergenic 40.0%
!! CTTGGTTCTGATCTATGCTG+TGG + chr4_1:75421353-75421372 Msa0572520:five_prime_UTR 45.0%
! AGAAGTTTGAGGTGGGGTTT+GGG - chr4_1:75421653-75421672 None:intergenic 45.0%
! AAGAAGTTTGAGGTGGGGTT+TGG - chr4_1:75421654-75421673 None:intergenic 45.0%
TGATTGGGTACCAAACCCAT+TGG + chr4_1:75421913-75421932 Msa0572520:CDS 45.0%
GATTGGGTACCAAACCCATT+GGG + chr4_1:75421914-75421933 Msa0572520:CDS 45.0%
AGGTTTCTCAACTTGATGCG+TGG + chr4_1:75422073-75422092 Msa0572520:CDS 45.0%
!! GGTAAAGTATGGGCACTGAT+TGG + chr4_1:75422094-75422113 Msa0572520:CDS 45.0%
ATAGCACCTGAGAATCGGAT+TGG + chr4_1:75422669-75422688 Msa0572520:intron 45.0%
TGTTGTCCAATCCGATTCTC+AGG - chr4_1:75422678-75422697 None:intergenic 45.0%
! ATCGGATTGGACAACAACTC+TGG + chr4_1:75422682-75422701 Msa0572520:intron 45.0%
! TCGGATTGGACAACAACTCT+GGG + chr4_1:75422683-75422702 Msa0572520:intron 45.0%
GTAATGGCTTGAACAAGCTG+AGG + chr4_1:75422971-75422990 Msa0572520:intron 45.0%
GTAATGGCTTGAACAAGCTG+AGG + chr4_1:75422971-75422990 Msa0572520:intron 45.0%
CATACACGTATAGTTGCCAC+CGG + chr4_1:75423113-75423132 Msa0572520:intron 45.0%
TGCATAATTGACTAGACCGG+TGG - chr4_1:75423132-75423151 None:intergenic 45.0%
TGTCTTAGTCTACCACGATG+AGG + chr4_1:75423577-75423596 Msa0572520:CDS 45.0%
GTCTTAGTCTACCACGATGA+GGG + chr4_1:75423578-75423597 Msa0572520:CDS 45.0%
AGGGATACAAGTCCAAAAGC+TGG + chr4_1:75423597-75423616 Msa0572520:CDS 45.0%
!!! TGAAGGAAAGTGCCAGCTTT+TGG - chr4_1:75423612-75423631 None:intergenic 45.0%
ATAGCAGCTTCCACAAGGAA+TGG - chr4_1:75423665-75423684 None:intergenic 45.0%
GACACTATATTGGGCTGTCA+CGG + chr4_1:75423836-75423855 Msa0572520:CDS 45.0%
AGTCAAACCCTAGATCTGTC+AGG - chr4_1:75423880-75423899 None:intergenic 45.0%
TCTGGTCTCAAAAGCGAAAG+TGG - chr4_1:75424178-75424197 None:intergenic 45.0%
!!! CTTTCGCTTTTGAGACCAGA+TGG + chr4_1:75424178-75424197 Msa0572520:CDS 45.0%
TGAAATTGCCTGTATGGACC+CGG - chr4_1:75424208-75424227 None:intergenic 45.0%
GCAATTTCAACCCTTTGCCA+AGG + chr4_1:75424219-75424238 Msa0572520:CDS 45.0%
! TGTTTGCATTGGTGCTTACC+TGG + chr4_1:75424268-75424287 Msa0572520:CDS 45.0%
! GTTTGCATTGGTGCTTACCT+GGG + chr4_1:75424269-75424288 Msa0572520:CDS 45.0%
CACATGCACAACTAGCATGA+GGG - chr4_1:75424383-75424402 None:intergenic 45.0%
CTCATGCTAGTTGTGCATGT+GGG + chr4_1:75424382-75424401 Msa0572520:three_prime_UTR 45.0%
! GTTTGGGGTTCAGTGAGAGT+GGG - chr4_1:75421637-75421656 None:intergenic 50.0%
! GAAGTTTGAGGTGGGGTTTG+GGG - chr4_1:75421652-75421671 None:intergenic 50.0%
!! TGTTACTGACCAGTGTCGGT+GGG - chr4_1:75421736-75421755 None:intergenic 50.0%
! ATGTTACTGACCAGTGTCGG+TGG - chr4_1:75421737-75421756 None:intergenic 50.0%
TGCAGAGCAAGGACTGAACA+TGG - chr4_1:75422138-75422157 None:intergenic 50.0%
! CTTAGAAAGCGTGCAGAGCA+AGG - chr4_1:75422149-75422168 None:intergenic 50.0%
CTTGCTCTGCACGCTTTCTA+AGG + chr4_1:75422147-75422166 Msa0572520:CDS 50.0%
!! TTTTCGGCTGATGCGTTGAC+CGG + chr4_1:75422334-75422353 Msa0572520:intron 50.0%
!! GCGTTGACCGGATCTTGTTT+TGG + chr4_1:75422346-75422365 Msa0572520:intron 50.0%
GCCAGTTACAAGTCCTAGAC+TGG + chr4_1:75422993-75423012 Msa0572520:intron 50.0%
! GCCAGTCTAGGACTTGTAAC+TGG - chr4_1:75422997-75423016 None:intergenic 50.0%
CGGTGGCAACTATACGTGTA+TGG - chr4_1:75423115-75423134 None:intergenic 50.0%
TGGACTTGTATCCCTCATCG+TGG - chr4_1:75423592-75423611 None:intergenic 50.0%
AATGGAGAGTGGTTTAGCGG+CGG + chr4_1:75424001-75424020 Msa0572520:CDS 50.0%
! TTTGAGACCAGATGGGCATC+CGG + chr4_1:75424186-75424205 Msa0572520:CDS 50.0%
TACCTGGGCCTATAGACTCT+TGG + chr4_1:75424284-75424303 Msa0572520:CDS 50.0%
TTCCAAGAGTCTATAGGCCC+AGG - chr4_1:75424289-75424308 None:intergenic 50.0%
CCACATGCACAACTAGCATG+AGG - chr4_1:75424384-75424403 None:intergenic 50.0%
CCTCATGCTAGTTGTGCATG+TGG + chr4_1:75424381-75424400 Msa0572520:three_prime_UTR 50.0%
! GGTTTGGGGTTCAGTGAGAG+TGG - chr4_1:75421638-75421657 None:intergenic 55.0%
TTGAGACCAGATGGGCATCC+GGG + chr4_1:75424187-75424206 Msa0572520:CDS 55.0%
CAACTCTCTCCCACCGACAC+TGG + chr4_1:75421724-75421743 Msa0572520:CDS 60.0%
TATGGACCCGGATGCCCATC+TGG - chr4_1:75424196-75424215 None:intergenic 60.0%
ATGGGCATCCGGGTCCATAC+AGG + chr4_1:75424197-75424216 Msa0572520:CDS 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 75421338 75424545 75421338 ID=Msa0572520;Name=Msa0572520
chr4_1 mRNA 75421338 75424545 75421338 ID=Msa0572520-mRNA-1;Parent=Msa0572520;Name=Msa0572520-mRNA-1;_AED=0.08;_eAED=0.08;_QI=185|1|1|1|1|1|3|203|425
chr4_1 exon 75421338 75421745 75421338 ID=Msa0572520-mRNA-1:exon:26127;Parent=Msa0572520-mRNA-1
chr4_1 exon 75421891 75422168 75421891 ID=Msa0572520-mRNA-1:exon:26128;Parent=Msa0572520-mRNA-1
chr4_1 exon 75423566 75424545 75423566 ID=Msa0572520-mRNA-1:exon:26129;Parent=Msa0572520-mRNA-1
chr4_1 five_prime_UTR 75421338 75421522 75421338 ID=Msa0572520-mRNA-1:five_prime_utr;Parent=Msa0572520-mRNA-1
chr4_1 CDS 75421523 75421745 75421523 ID=Msa0572520-mRNA-1:cds;Parent=Msa0572520-mRNA-1
chr4_1 CDS 75421891 75422168 75421891 ID=Msa0572520-mRNA-1:cds;Parent=Msa0572520-mRNA-1
chr4_1 CDS 75423566 75424342 75423566 ID=Msa0572520-mRNA-1:cds;Parent=Msa0572520-mRNA-1
chr4_1 three_prime_UTR 75424343 75424545 75424343 ID=Msa0572520-mRNA-1:three_prime_utr;Parent=Msa0572520-mRNA-1
Gene Sequence

>Msa0572520

ATGAGGATCGATTCTTCTTTCAATTTCAACCATAAAAATTACCTCATCAAGTTACTTCTCACTCTTTTCTTCTCTGTACTCGCTTTTCGTCTTCTCTTCTTTCAATCTCTTCCCACTCTCACTGAACCCCAAACCCCACCTCAAACTTCTTCTCTTTCTGACCTTCAACAACTCCCTCTTCAAAATGAACAAGAACAAGATCAACTCTCTCCCACCGACACTGGTAAATGTGATTATTTTAATGGTGATTGGGTACCAAACCCATTGGGTCCAATTTATACAAATGAAACATGTGACCTCATTGAATCACATCAAAATTGCTTGAAAAATGGGAGACCAGACAGAGATTTTTTGTACTGGAAGTGGGCTCCTAGAGATTGTGAGTTACCTATTTTTGATCCTCAAAGGTTTCTCAACTTGATGCGTGGTAAAGTATGGGCACTGATTGGTGATTCCATTTCTCGTAACCATGTTCAGTCCTTGCTCTGCACGCTTTCTAAGGTAGAAAAAGCTGTCTTAGTCTACCACGATGAGGGATACAAGTCCAAAAGCTGGCACTTTCCTTCATACAACTTCAGTATGTCAGTTATTTGGTCACCATTCCTTGTGGAAGCTGCTATTTTTGAAGACATAAACGGCGTTTCAAGTTCTGAAGTTGAGCTATATCTCGACAAACTGGATAGTAAATGGACAGATCAGTATCTGAACTTCGATTACATTATTATTTCAACGGGAAAGTGGTTTGTCAAATCTGCAATCTACTATGAGAATGACACTATATTGGGCTGTCACGGCTGTTCAAGTAACAAGAACCTGACAGATCTAGGGTTTGACTTTGCATATCGCAAAGTCTTGAAAAATGTCATGAACTTCATAGTCTCTTCCAATCACAAGGGCTTGATTTTGTTCAGGACATCCACACCTGACCATTTTGAAAATGGAGAGTGGTTTAGCGGCGGAACGTGCAACAGAACTGAACCGATTAAAGAAGGTGAAATGGAAATTAAGCTTTTGCTTAGGATTCTTCGAGACATTGAGTTAGAAGAGTTTAAAAAGGCAGCGTCTAAAGCTTCAAAAAATGGAGTGAATCTCAAGCTTGCTGATTTTGCACCACTTTCGCTTTTGAGACCAGATGGGCATCCGGGTCCATACAGGCAATTTCAACCCTTTGCCAAGGACAAAAATGCTAAAGTTCAGACTGATTGTTTGCATTGGTGCTTACCTGGGCCTATAGACTCTTGGAATGACATCATAATGGAGATGGTTGTCAATGGATAA
Protein sequence

>Msa0572520

MRIDSSFNFNHKNYLIKLLLTLFFSVLAFRLLFFQSLPTLTEPQTPPQTSSLSDLQQLPLQNEQEQDQLSPTDTGKCDYFNGDWVPNPLGPIYTNETCDLIESHQNCLKNGRPDRDFLYWKWAPRDCELPIFDPQRFLNLMRGKVWALIGDSISRNHVQSLLCTLSKVEKAVLVYHDEGYKSKSWHFPSYNFSMSVIWSPFLVEAAIFEDINGVSSSEVELYLDKLDSKWTDQYLNFDYIIISTGKWFVKSAIYYENDTILGCHGCSSNKNLTDLGFDFAYRKVLKNVMNFIVSSNHKGLILFRTSTPDHFENGEWFSGGTCNRTEPIKEGEMEIKLLLRILRDIELEEFKKAASKASKNGVNLKLADFAPLSLLRPDGHPGPYRQFQPFAKDKNAKVQTDCLHWCLPGPIDSWNDIIMEMVVNG*