AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0577860

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0577860 MtrunA17_Chr1g0201781 93.143 175 6 4 1 175 1 169 1.13e-90 261
Msa0577860 MtrunA17_Chr1g0211871 38.614 101 60 1 74 172 76 176 4.52e-24 92.8
Msa0577860 MtrunA17_Chr3g0125821 36.634 101 64 0 72 172 74 174 1.98e-22 88.6
Msa0577860 MtrunA17_Chr8g0360031 36.082 97 62 0 72 168 77 173 4.97e-22 87.4
Msa0577860 MtrunA17_Chr2g0317731 30.065 153 95 2 28 168 18 170 5.20e-22 87.4
Msa0577860 MtrunA17_Chr1g0155721 40.789 76 44 1 82 157 26 100 1.98e-19 79.3
Msa0577860 MtrunA17_Chr7g0216481 35.955 89 55 2 80 168 44 130 7.31e-19 78.2
Msa0577860 MtrunA17_Chr3g0142051 38.158 76 46 1 82 157 26 100 1.65e-18 76.6
Msa0577860 MtrunA17_Chr4g0044731 29.747 158 98 3 19 172 21 169 1.21e-17 75.9
Msa0577860 MtrunA17_Chr5g0404831 35.644 101 60 3 71 168 14 112 2.03e-17 73.9
Msa0577860 MtrunA17_Chr5g0415771 38.095 63 38 1 83 145 40 101 1.00e-15 69.7
Msa0577860 MtrunA17_Chr2g0280411 35.227 88 55 2 81 168 28 113 4.92e-14 65.5
Msa0577860 MtrunA17_Chr1g0194961 31.915 94 61 2 74 164 155 248 7.67e-14 68.2
Msa0577860 MtrunA17_Chr1g0194961 34.343 99 58 4 82 174 44 141 7.63e-11 59.7
Msa0577860 MtrunA17_Chr5g0438731 31.915 94 62 1 64 157 60 151 1.12e-13 68.2
Msa0577860 MtrunA17_Chr5g0438731 29.808 104 66 3 74 171 198 300 7.87e-12 62.8
Msa0577860 MtrunA17_Chr1g0200731 36.735 98 57 3 74 168 101 196 1.25e-13 67.8
Msa0577860 MtrunA17_Chr4g0042051 34.066 91 58 2 82 172 30 118 8.26e-13 62.0
Msa0577860 MtrunA17_Chr5g0415761 30.556 72 49 1 73 144 31 101 1.05e-12 62.0
Msa0577860 MtrunA17_Chr2g0287471 31.325 83 57 0 75 157 37 119 3.89e-12 63.2
Msa0577860 MtrunA17_Chr2g0317701 29.167 120 80 2 57 171 31 150 1.02e-11 59.7
Msa0577860 MtrunA17_Chr5g0415741 32.258 62 41 1 83 144 39 99 1.39e-11 58.9
Msa0577860 MtrunA17_Chr2g0317721 29.897 97 68 0 75 171 48 144 5.57e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0577860 AT1G76760.1 62.209 172 63 1 3 174 2 171 4.48e-67 202
Msa0577860 AT1G43560.1 64.744 156 48 4 25 175 14 167 4.45e-62 189
Msa0577860 AT1G50320.1 44.554 101 54 1 74 172 77 177 6.24e-26 98.2
Msa0577860 AT1G03680.1 35.714 98 63 0 74 171 82 179 2.16e-22 89.0
Msa0577860 AT3G15360.1 38.542 96 56 1 74 166 94 189 3.18e-20 83.6
Msa0577860 AT5G39950.1 35.227 88 55 2 80 167 43 128 1.76e-17 74.7
Msa0577860 AT4G03520.1 32.941 85 57 0 74 158 88 172 4.28e-17 75.1
Msa0577860 AT3G51030.1 41.026 78 44 2 81 157 24 100 7.57e-17 72.8
Msa0577860 AT1G59730.1 32.039 103 67 3 66 168 26 125 2.89e-16 71.6
Msa0577860 AT2G15570.1 28.302 106 76 0 67 172 67 172 5.08e-16 72.0
Msa0577860 AT2G15570.2 28.571 105 75 0 68 172 69 173 8.59e-16 71.6
Msa0577860 AT1G45145.1 35.294 85 53 2 83 167 26 108 2.45e-15 68.9
Msa0577860 AT1G19730.1 30.952 84 58 0 78 161 22 105 2.88e-14 66.2
Msa0577860 AT3G56420.1 36.250 80 50 1 82 161 60 138 8.76e-14 65.9
Msa0577860 AT3G56420.2 36.250 80 50 1 82 161 60 138 8.76e-14 65.9
Msa0577860 AT3G56420.3 36.250 80 50 1 82 161 60 138 8.76e-14 65.9
Msa0577860 AT1G04980.1 34.831 89 58 0 72 160 38 126 2.01e-13 67.8
Msa0577860 AT1G35620.1 37.500 96 55 3 72 164 38 131 3.75e-13 67.0
Msa0577860 AT5G42980.1 34.211 76 49 1 82 157 25 99 9.62e-13 62.0
Msa0577860 AT2G47470.1 29.032 93 63 2 75 164 150 242 1.03e-12 65.5
Msa0577860 AT2G47470.3 29.032 93 63 2 75 164 150 242 1.24e-12 65.1
Msa0577860 AT2G47470.4 29.032 93 63 2 75 164 150 242 1.30e-12 65.1
Msa0577860 AT3G06730.1 32.353 170 103 6 11 171 17 183 1.60e-12 63.2
Msa0577860 AT1G69880.1 32.258 93 57 2 80 172 55 141 1.62e-12 62.4
Msa0577860 AT2G47470.2 29.167 96 65 2 75 167 150 245 1.91e-12 63.9
Msa0577860 AT2G32920.1 36.735 98 57 3 64 160 31 124 3.10e-12 64.3
Msa0577860 AT2G32920.1 30.337 89 59 2 74 160 170 257 8.89e-11 60.1
Msa0577860 AT3G17880.2 37.313 67 41 1 91 157 292 357 3.52e-12 63.9
Msa0577860 AT3G54960.2 32.258 93 62 1 72 164 105 196 3.80e-12 63.9
Msa0577860 AT3G17880.1 37.313 67 41 1 91 157 299 364 4.16e-12 63.5
Msa0577860 AT3G08710.2 33.708 89 55 2 72 157 30 117 6.81e-12 60.5
Msa0577860 AT3G08710.3 33.708 89 55 2 72 157 30 117 6.81e-12 60.5
Msa0577860 AT3G08710.1 33.708 89 55 2 72 157 30 117 6.81e-12 60.5
Msa0577860 AT5G16400.1 37.079 89 53 3 81 168 94 180 2.50e-11 59.7
Msa0577860 AT3G54960.1 32.258 93 62 1 72 164 105 196 3.39e-11 61.2
Msa0577860 AT1G11530.1 29.885 87 59 2 82 168 22 106 6.30e-11 57.4

Find 68 sgRNAs with CRISPR-Local

Find 239 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCAACTTTCATCATCTTTA+AGG 0.037363 4_1:+82172082 Msa0577860:CDS
TATCTACTTATTTATTTCTT+TGG 0.125239 4_1:-82170288 None:intergenic
ATCTACTTATTTATTTCTTT+GGG 0.274403 4_1:-82170287 None:intergenic
GTGTGGTCCTTGTCAGTTCA+TGG 0.318473 4_1:+82171950 Msa0577860:CDS
AACAATGTGCAAGTCATATT+AGG 0.342152 4_1:+82173701 Msa0577860:three_prime_UTR
CTGTGGTCAGGTTCAATTTG+TGG 0.343285 4_1:-82173662 None:intergenic
TGCCTCAATGTTGTATTTGT+TGG 0.387952 4_1:-82172057 None:intergenic
AACCGTACGAAAGTTGGATT+TGG 0.396179 4_1:+82173624 Msa0577860:three_prime_UTR
GAAACGAGGGCGGGAGGAAG+AGG 0.407611 4_1:-82170522 None:intergenic
CTCTTCTTCGTCCACACGTC+TGG 0.425949 4_1:+82170415 Msa0577860:CDS
GGAAAACCAACTGCTCTGTC+TGG 0.432185 4_1:-82170234 None:intergenic
TTTATTTCTTTGGGAATTGA+CGG 0.439793 4_1:-82170278 None:intergenic
CAAATCATCAAAAGAGGAAA+AGG 0.443022 4_1:-82171716 None:intergenic
ACTCCTCGCCTTCTTCCAAT+TGG 0.450067 4_1:+82170485 Msa0577860:CDS
AATTGCAACCGTACGAAAGT+TGG 0.455861 4_1:+82173618 Msa0577860:three_prime_UTR
TGCTGAGATGGAAGCGGCCA+TGG 0.463484 4_1:-82170360 None:intergenic
GCATAGAAGTCAACAAATAC+AGG 0.470351 4_1:-82171757 None:intergenic
AGACATAAGCACCTGTGGTC+AGG 0.473819 4_1:-82173674 None:intergenic
AGTTTGTCAATCGGAATGTT+GGG 0.474535 4_1:+82173342 Msa0577860:three_prime_UTR
CAAAATGAGAACGAACAGAT+TGG 0.476905 4_1:-82170333 None:intergenic
AGAAATTAATATTTAATCCC+AGG 0.480275 4_1:-82170255 None:intergenic
AGGAAGTAGGAAACGAGGGC+GGG 0.481188 4_1:-82170531 None:intergenic
GAGTTTGTCAATCGGAATGT+TGG 0.483482 4_1:+82173341 Msa0577860:three_prime_UTR
GAAAGACGGCGAGTACCAAT+TGG 0.490211 4_1:-82170500 None:intergenic
CAACTTACAAAGCGATCAAA+GGG 0.495557 4_1:-82172113 None:intergenic
CACATACATGGTTGGATAGG+AGG 0.496043 4_1:+82173367 Msa0577860:three_prime_UTR
GTCAACAAATACAGGCTTGT+CGG 0.497227 4_1:-82171749 None:intergenic
TCCTTAAAGATGATGAAAGT+TGG 0.497295 4_1:-82172083 None:intergenic
ACTTTCATCATCTTTAAGGA+TGG 0.499489 4_1:+82172086 Msa0577860:CDS
AGAGGAAGACGCTGCTGAGA+TGG 0.502504 4_1:-82170372 None:intergenic
TGCAAGCAAATCATCAAAAG+AGG 0.504002 4_1:-82171722 None:intergenic
GACCACAGGTGCTTATGTCT+TGG 0.514527 4_1:+82173677 Msa0577860:three_prime_UTR
CCAACTTACAAAGCGATCAA+AGG 0.517391 4_1:-82172114 None:intergenic
GGCGAGGAGTTCGAAATTGA+AGG 0.521846 4_1:-82170472 None:intergenic
AAGTAGGAAACGAGGGCGGG+AGG 0.525596 4_1:-82170528 None:intergenic
GGCGGGAGGAAGAGGAAAGA+CGG 0.525873 4_1:-82170514 None:intergenic
AAAAGTCCAGACAGAGCAGT+TGG 0.528989 4_1:+82170228 Msa0577860:five_prime_UTR
AGGAGTGAAAGTGAGAGGAA+AGG 0.533074 4_1:+82170199 None:intergenic
AACCAAATCCAACTTTCGTA+CGG 0.546199 4_1:-82173626 None:intergenic
GTTCATGGTTCCTGTACTTG+AGG 0.555079 4_1:+82171965 Msa0577860:CDS
GCGCTCACTTCCTCAAGTAC+AGG 0.556326 4_1:-82171975 None:intergenic
GTTGGGATCACATACATGGT+TGG 0.565508 4_1:+82173359 Msa0577860:three_prime_UTR
AGACGCTGCTGAGATGGAAG+CGG 0.566090 4_1:-82170366 None:intergenic
ACGAAGAAGAGTTCAAAGAA+TGG 0.574201 4_1:-82170403 None:intergenic
GATCACATACATGGTTGGAT+AGG 0.582401 4_1:+82173364 Msa0577860:three_prime_UTR
TTTCAGTTAAATGAAGACAC+AGG 0.583049 4_1:+82173319 Msa0577860:intron
CACAAATTGAACCTGACCAC+AGG 0.583927 4_1:+82173663 Msa0577860:three_prime_UTR
GAGGAAGTAGGAAACGAGGG+CGG 0.585576 4_1:-82170532 None:intergenic
GGCGAGTACCAATTGGAAGA+AGG 0.589024 4_1:-82170493 None:intergenic
GTAGAAGGAGTGAAAGTGAG+AGG 0.590737 4_1:+82170194 None:intergenic
TTGTATTTGTTGGCAATGCT+AGG 0.592667 4_1:-82172047 None:intergenic
AGACACAGGAGTTTGTCAAT+CGG 0.598830 4_1:+82173333 Msa0577860:three_prime_UTR
TTGTTGACTTCTATGCAACC+TGG 0.598842 4_1:+82171764 Msa0577860:CDS
GAATGTTGGGATCACATACA+TGG 0.601877 4_1:+82173355 Msa0577860:three_prime_UTR
TTATTGCTTAACGTTGAGAG+TGG 0.611598 4_1:-82173165 None:intergenic
TACTTGAGGAAGTGAGCGCT+CGG 0.618555 4_1:+82171979 Msa0577860:CDS
TCAACGTTAAGCAATAACTC+TGG 0.625204 4_1:+82173171 Msa0577860:exon
TGTTACTTACGAGAGGAAGT+AGG 0.626215 4_1:-82170544 None:intergenic
AAGAAGAGTTCAAAGAATGG+AGG 0.632769 4_1:-82170400 None:intergenic
GTACCAATTGGAAGAAGGCG+AGG 0.648368 4_1:-82170488 None:intergenic
TGCCAAGACATAAGCACCTG+TGG 0.649363 4_1:-82173679 None:intergenic
CGAGAGGAAGTAGGAAACGA+GGG 0.659041 4_1:-82170535 None:intergenic
TGCCAACAAATACAACATTG+AGG 0.660640 4_1:+82172055 Msa0577860:CDS
ATTGGATAATCAGAAAACAA+TGG 0.661635 4_1:-82170315 None:intergenic
CAAAGAATGGAGGATTACAG+AGG 0.663758 4_1:-82170390 None:intergenic
ACAGGAACCATGAACTGACA+AGG 0.685218 4_1:-82171957 None:intergenic
ACGAGAGGAAGTAGGAAACG+AGG 0.685474 4_1:-82170536 None:intergenic
TGATGAAGGTACCAGACGTG+TGG 0.715833 4_1:-82170426 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATAATGGTTATTATAAAA+CGG - chr4_1:82170606-82170625 None:intergenic 10.0%
!! AATTCAATAAGTATATATAT+GGG - chr4_1:82170977-82170996 None:intergenic 10.0%
!! AAATTCAATAAGTATATATA+TGG - chr4_1:82170978-82170997 None:intergenic 10.0%
!!! TAAACAATCAAATTTTTTAT+TGG - chr4_1:82173463-82173482 None:intergenic 10.0%
!!! AATATAATTTAGTTTTGATT+TGG + chr4_1:82173581-82173600 Msa0577860:three_prime_UTR 10.0%
!!! ATATAATTTAGTTTTGATTT+GGG + chr4_1:82173582-82173601 Msa0577860:three_prime_UTR 10.0%
!!! ATCTACTTATTTATTTCTTT+GGG - chr4_1:82170290-82170309 None:intergenic 15.0%
!!! TATCTACTTATTTATTTCTT+TGG - chr4_1:82170291-82170310 None:intergenic 15.0%
!!! ATTTTATTTATACTTGTTGT+TGG + chr4_1:82170648-82170667 Msa0577860:intron 15.0%
!!! TAATTTTTTTGGAATACAAA+AGG + chr4_1:82171269-82171288 Msa0577860:intron 15.0%
!! AATATACAAAACATCAATTT+AGG - chr4_1:82173011-82173030 None:intergenic 15.0%
!! CAAGTATCATTTAAATAAAA+TGG - chr4_1:82173229-82173248 None:intergenic 15.0%
!!! TATAATTTAGTTTTGATTTG+GGG + chr4_1:82173583-82173602 Msa0577860:three_prime_UTR 15.0%
!! AGAAATTAATATTTAATCCC+AGG - chr4_1:82170258-82170277 None:intergenic 20.0%
!! AAGGAGAAAAATAAAATAGT+TGG - chr4_1:82170577-82170596 None:intergenic 20.0%
!! AAATGAATTAGTGAATAACT+TGG - chr4_1:82170679-82170698 None:intergenic 20.0%
!! AATAAGTATATATATGGGTT+TGG - chr4_1:82170972-82170991 None:intergenic 20.0%
!!! ATCCAATTTTTTCCTATATT+TGG + chr4_1:82171214-82171233 Msa0577860:intron 20.0%
!! ATCCAAATATAGGAAAAAAT+TGG - chr4_1:82171219-82171238 None:intergenic 20.0%
!! AAAAAAATTAGCGTAAATTC+TGG - chr4_1:82171259-82171278 None:intergenic 20.0%
!!! AGAATTTACGCTAATTTTTT+TGG + chr4_1:82171258-82171277 Msa0577860:intron 20.0%
!! AACTACAAATTTACTAAATG+CGG + chr4_1:82171295-82171314 Msa0577860:intron 20.0%
!! TCAGTAAGTTAAAAGAATAT+CGG - chr4_1:82172278-82172297 None:intergenic 20.0%
!!! TTATAGATAGTTAGTTTCAA+TGG + chr4_1:82172337-82172356 Msa0577860:intron 20.0%
!!! AAAATACACAGAAATCTTTT+CGG + chr4_1:82172442-82172461 Msa0577860:intron 20.0%
!! ACAATTTACAACAATAAGAA+AGG - chr4_1:82172825-82172844 None:intergenic 20.0%
!!! CATGCATATAATTAATTTGT+CGG + chr4_1:82172902-82172921 Msa0577860:intron 20.0%
!!! ACCAAAATTTTATCTAACTT+GGG - chr4_1:82173087-82173106 None:intergenic 20.0%
!!! ATGTACAAGAATTTTGATAT+TGG - chr4_1:82173559-82173578 None:intergenic 20.0%
!!! ATAATTTAGTTTTGATTTGG+GGG + chr4_1:82173584-82173603 Msa0577860:three_prime_UTR 20.0%
!! TTTATTTCTTTGGGAATTGA+CGG - chr4_1:82170281-82170300 None:intergenic 25.0%
! ATTGGATAATCAGAAAACAA+TGG - chr4_1:82170318-82170337 None:intergenic 25.0%
! TATTATAAAACGGAAAGGAA+AGG - chr4_1:82170596-82170615 None:intergenic 25.0%
! ATGGTTATTATAAAACGGAA+AGG - chr4_1:82170601-82170620 None:intergenic 25.0%
!! TGAAATATATAGTGTTGGAA+GGG - chr4_1:82170766-82170785 None:intergenic 25.0%
! TTCCAACACTATATATTTCA+CGG + chr4_1:82170766-82170785 Msa0577860:intron 25.0%
! AATCAAAAGGTCTAAGTTTA+GGG - chr4_1:82170846-82170865 None:intergenic 25.0%
! AATTTAAGCACTTATAAGCA+GGG + chr4_1:82170993-82171012 Msa0577860:intron 25.0%
! ACTGTTAAACTGTTAAACTA+TGG + chr4_1:82171553-82171572 Msa0577860:intron 25.0%
! AAAACAATATCCAAAAATGC+TGG - chr4_1:82171671-82171690 None:intergenic 25.0%
! AGTAAGTTAAAAGAATATCG+GGG - chr4_1:82172276-82172295 None:intergenic 25.0%
! CAGTAAGTTAAAAGAATATC+GGG - chr4_1:82172277-82172296 None:intergenic 25.0%
! AATCAAATGCAGTATCATTA+TGG - chr4_1:82172407-82172426 None:intergenic 25.0%
! ATCATGTCTTAACCAAAATT+AGG - chr4_1:82172552-82172571 None:intergenic 25.0%
!!! TTTTGGTTAAGACATGATAT+TGG + chr4_1:82172554-82172573 Msa0577860:intron 25.0%
! ACTTCAACATAATGAAATTG+AGG + chr4_1:82172613-82172632 Msa0577860:intron 25.0%
! TTAGAATGTAAAGAACCTAA+AGG + chr4_1:82172645-82172664 Msa0577860:intron 25.0%
! TCTACTTAAAAACAACCTTT+AGG - chr4_1:82172663-82172682 None:intergenic 25.0%
!! CCAAAATTTTATCTAACTTG+GGG - chr4_1:82173086-82173105 None:intergenic 25.0%
!! GACCAAAATTTTATCTAACT+TGG - chr4_1:82173088-82173107 None:intergenic 25.0%
! ATTTAAATGATACTTGCTTC+AGG + chr4_1:82173233-82173252 Msa0577860:intron 25.0%
! TTTAACTGAAAAAAGTAGCA+AGG - chr4_1:82173310-82173329 None:intergenic 25.0%
!!! CAATCAAATTTTTTATTGGC+TGG - chr4_1:82173459-82173478 None:intergenic 25.0%
GGTAATATGATTCAGAAATG+AGG - chr4_1:82170745-82170764 None:intergenic 30.0%
! GTGAAATATATAGTGTTGGA+AGG - chr4_1:82170767-82170786 None:intergenic 30.0%
CAATCAAAAGGTCTAAGTTT+AGG - chr4_1:82170847-82170866 None:intergenic 30.0%
GAATTTAAGCACTTATAAGC+AGG + chr4_1:82170992-82171011 Msa0577860:intron 30.0%
ATTATGTCGTGTCAAAATTG+CGG + chr4_1:82171114-82171133 Msa0577860:intron 30.0%
!! TGTAAGCAGGGTTTTAATAA+AGG - chr4_1:82171147-82171166 None:intergenic 30.0%
! ACTGTTAAACTATGGCTTTA+AGG + chr4_1:82171561-82171580 Msa0577860:intron 30.0%
! TTTTGGATATTGTTTTCTGC+AGG + chr4_1:82171675-82171694 Msa0577860:intron 30.0%
CAAATCATCAAAAGAGGAAA+AGG - chr4_1:82171719-82171738 None:intergenic 30.0%
CATGAAATATAACCAGCTAA+AGG - chr4_1:82171856-82171875 None:intergenic 30.0%
TCCTTAAAGATGATGAAAGT+TGG - chr4_1:82172086-82172105 None:intergenic 30.0%
! ACTTTCATCATCTTTAAGGA+TGG + chr4_1:82172086-82172105 Msa0577860:CDS 30.0%
CATGAAAAATTGTGATGTCA+AGG + chr4_1:82172374-82172393 Msa0577860:intron 30.0%
! TTCTGTGTATTTTCTATGAC+TGG - chr4_1:82172435-82172454 None:intergenic 30.0%
CTTAACCAAAATTAGGAACT+AGG - chr4_1:82172545-82172564 None:intergenic 30.0%
! TAAGTAGAAACTTTTGTCTG+TGG + chr4_1:82172675-82172694 Msa0577860:intron 30.0%
CGTCATATTTGAAAAAGTGA+AGG + chr4_1:82172745-82172764 Msa0577860:intron 30.0%
TTGAAAAAGTGAAGGAATGA+AGG + chr4_1:82172753-82172772 Msa0577860:intron 30.0%
TAAATCTCATCACAGTCATT+TGG + chr4_1:82172854-82172873 Msa0577860:intron 30.0%
TGACAAGACAAAAGAAAACT+AGG + chr4_1:82173031-82173050 Msa0577860:intron 30.0%
ACAAAAGAAAACTAGGATGT+TGG + chr4_1:82173038-82173057 Msa0577860:intron 30.0%
! AATTTTATCTAACTTGGGGA+AGG - chr4_1:82173082-82173101 None:intergenic 30.0%
! CCCCAAGTTAGATAAAATTT+TGG + chr4_1:82173083-82173102 Msa0577860:intron 30.0%
!! TTTTGGTCTTCATGATTTTC+AGG + chr4_1:82173100-82173119 Msa0577860:intron 30.0%
! ATAAAATGGTACTGAATCGA+TGG - chr4_1:82173215-82173234 None:intergenic 30.0%
! AAAGTAGCAAGGTATTTAGA+GGG - chr4_1:82173299-82173318 None:intergenic 30.0%
! AAAAGTAGCAAGGTATTTAG+AGG - chr4_1:82173300-82173319 None:intergenic 30.0%
TTTCAGTTAAATGAAGACAC+AGG + chr4_1:82173319-82173338 Msa0577860:intron 30.0%
TGGATCTTGGCAAAAATAAT+GGG - chr4_1:82173439-82173458 None:intergenic 30.0%
!!! ATTTTTTATTGGCTGGATCT+TGG - chr4_1:82173452-82173471 None:intergenic 30.0%
CAAAATGAGAACGAACAGAT+TGG - chr4_1:82170336-82170355 None:intergenic 35.0%
AAGAAGAGTTCAAAGAATGG+AGG - chr4_1:82170403-82170422 None:intergenic 35.0%
ACGAAGAAGAGTTCAAAGAA+TGG - chr4_1:82170406-82170425 None:intergenic 35.0%
! GATGATGATGATGATGATGA+AGG - chr4_1:82170443-82170462 None:intergenic 35.0%
!! TTAGTAGTGTTACTTACGAG+AGG - chr4_1:82170554-82170573 None:intergenic 35.0%
GTCCGTGAAATATATAGTGT+TGG - chr4_1:82170771-82170790 None:intergenic 35.0%
CACTATATATTTCACGGACT+AGG + chr4_1:82170772-82170791 Msa0577860:intron 35.0%
!!! TAGGCTTTTGTTTTGGTGTT+TGG + chr4_1:82170791-82170810 Msa0577860:intron 35.0%
CCATCCACCAAAAATCTTTA+GGG + chr4_1:82170867-82170886 Msa0577860:intron 35.0%
! AACCAGACCCTAAAGATTTT+TGG - chr4_1:82170877-82170896 None:intergenic 35.0%
!! AAAAATCTTTAGGGTCTGGT+TGG + chr4_1:82170876-82170895 Msa0577860:intron 35.0%
CTGCAATTGCAATCATGTTA+TGG + chr4_1:82171070-82171089 Msa0577860:intron 35.0%
GAATTTATGACGTGTAAGCA+GGG - chr4_1:82171159-82171178 None:intergenic 35.0%
AGAATTTATGACGTGTAAGC+AGG - chr4_1:82171160-82171179 None:intergenic 35.0%
! AATTATCGCCTGATTTGATC+AGG + chr4_1:82171346-82171365 Msa0577860:intron 35.0%
! ATTATCGCCTGATTTGATCA+GGG + chr4_1:82171347-82171366 Msa0577860:intron 35.0%
ATCAGGGCAATCATCATAAA+TGG + chr4_1:82171363-82171382 Msa0577860:intron 35.0%
TGCAAGCAAATCATCAAAAG+AGG - chr4_1:82171725-82171744 None:intergenic 35.0%
GCATAGAAGTCAACAAATAC+AGG - chr4_1:82171760-82171779 None:intergenic 35.0%
AGAAGAAAATGGAAAACGCA+CGG - chr4_1:82171801-82171820 None:intergenic 35.0%
ATGAAGAGAGGAGAAGAAAA+TGG - chr4_1:82171812-82171831 None:intergenic 35.0%
!!! GAAGCATTTTTTCCTTTAGC+TGG + chr4_1:82171841-82171860 Msa0577860:intron 35.0%
! TTGTATTTGTTGGCAATGCT+AGG - chr4_1:82172050-82172069 None:intergenic 35.0%
TGCCAACAAATACAACATTG+AGG + chr4_1:82172055-82172074 Msa0577860:CDS 35.0%
TGCCTCAATGTTGTATTTGT+TGG - chr4_1:82172060-82172079 None:intergenic 35.0%
GCCAACTTTCATCATCTTTA+AGG + chr4_1:82172082-82172101 Msa0577860:CDS 35.0%
CAACTTACAAAGCGATCAAA+GGG - chr4_1:82172116-82172135 None:intergenic 35.0%
TGTAGAAGACTTGTATGTAG+AGG + chr4_1:82172514-82172533 Msa0577860:intron 35.0%
! AATCGCCTAGTTCCTAATTT+TGG + chr4_1:82172537-82172556 Msa0577860:intron 35.0%
TGTCTGTGGTCTATAACATA+TGG + chr4_1:82172689-82172708 Msa0577860:intron 35.0%
TTATTGCTTAACGTTGAGAG+TGG - chr4_1:82173168-82173187 None:intergenic 35.0%
TCAACGTTAAGCAATAACTC+TGG + chr4_1:82173171-82173190 Msa0577860:exon 35.0%
AGTTTGTCAATCGGAATGTT+GGG + chr4_1:82173342-82173361 Msa0577860:three_prime_UTR 35.0%
CTGGATCTTGGCAAAAATAA+TGG - chr4_1:82173440-82173459 None:intergenic 35.0%
AACCAAATCCAACTTTCGTA+CGG - chr4_1:82173629-82173648 None:intergenic 35.0%
!!! CGTTCTCATTTTGACTACCA+TGG + chr4_1:82170343-82170362 Msa0577860:exon 40.0%
CAAAGAATGGAGGATTACAG+AGG - chr4_1:82170393-82170412 None:intergenic 40.0%
! TGTTACTTACGAGAGGAAGT+AGG - chr4_1:82170547-82170566 None:intergenic 40.0%
! TTTGGTGGATGGCAATCAAA+AGG - chr4_1:82170859-82170878 None:intergenic 40.0%
GCCATCCACCAAAAATCTTT+AGG + chr4_1:82170866-82170885 Msa0577860:intron 40.0%
!!! CCCTAAAGATTTTTGGTGGA+TGG - chr4_1:82170870-82170889 None:intergenic 40.0%
!! CAGACCCTAAAGATTTTTGG+TGG - chr4_1:82170874-82170893 None:intergenic 40.0%
!! CACCAAAAATCTTTAGGGTC+TGG + chr4_1:82170872-82170891 Msa0577860:intron 40.0%
! GTTGGTTCAAGTTCAACAGA+GGG + chr4_1:82170898-82170917 Msa0577860:intron 40.0%
! TTGGTTCAAGTTCAACAGAG+GGG + chr4_1:82170899-82170918 Msa0577860:intron 40.0%
!! AAATGGGTTTAGGGGGATTT+TGG + chr4_1:82170927-82170946 Msa0577860:intron 40.0%
!! AATGGGTTTAGGGGGATTTT+GGG + chr4_1:82170928-82170947 Msa0577860:intron 40.0%
TGGGGCAACAATCCAAATAT+AGG - chr4_1:82171229-82171248 None:intergenic 40.0%
GTAAATTCTGGCGATCAATG+GGG - chr4_1:82171247-82171266 None:intergenic 40.0%
CGTAAATTCTGGCGATCAAT+GGG - chr4_1:82171248-82171267 None:intergenic 40.0%
ATGATTGCCCTGATCAAATC+AGG - chr4_1:82171357-82171376 None:intergenic 40.0%
GAGTCATGTTTATCGTGAAG+CGG - chr4_1:82171443-82171462 None:intergenic 40.0%
AACGATTTAGCAGCTAGACT+GGG - chr4_1:82171476-82171495 None:intergenic 40.0%
AAACGATTTAGCAGCTAGAC+TGG - chr4_1:82171477-82171496 None:intergenic 40.0%
ATCTCGAGTATCAGAAGTAG+AGG - chr4_1:82171532-82171551 None:intergenic 40.0%
GTGCAAGCATGAATGAACAT+GGG - chr4_1:82171594-82171613 None:intergenic 40.0%
AGTGCAAGCATGAATGAACA+TGG - chr4_1:82171595-82171614 None:intergenic 40.0%
! TGAGAACTGACCAGCATTTT+TGG + chr4_1:82171658-82171677 Msa0577860:intron 40.0%
GTCAACAAATACAGGCTTGT+CGG - chr4_1:82171752-82171771 None:intergenic 40.0%
!! TTGTTGACTTCTATGCAACC+TGG + chr4_1:82171764-82171783 Msa0577860:CDS 40.0%
AAGAAAATGGAAAACGCACG+GGG - chr4_1:82171799-82171818 None:intergenic 40.0%
GAAGAAAATGGAAAACGCAC+GGG - chr4_1:82171800-82171819 None:intergenic 40.0%
TTCTTCTCCTCTCTTCATGT+TGG + chr4_1:82171814-82171833 Msa0577860:intron 40.0%
TCTTCTCCTCTCTTCATGTT+GGG + chr4_1:82171815-82171834 Msa0577860:intron 40.0%
TTCAAACCCAACATGAAGAG+AGG - chr4_1:82171824-82171843 None:intergenic 40.0%
TCTAATCAGTAACCCAATGC+TGG + chr4_1:82171920-82171939 Msa0577860:intron 40.0%
!! GTAACCCAATGCTGGTTTAT+AGG + chr4_1:82171928-82171947 Msa0577860:intron 40.0%
CACACCTATAAACCAGCATT+GGG - chr4_1:82171935-82171954 None:intergenic 40.0%
CCAACTTACAAAGCGATCAA+AGG - chr4_1:82172117-82172136 None:intergenic 40.0%
CCTTTGATCGCTTTGTAAGT+TGG + chr4_1:82172114-82172133 Msa0577860:intron 40.0%
! AAATCTTTTCGGCTTCTTGC+TGG + chr4_1:82172453-82172472 Msa0577860:intron 40.0%
! AATCTTTTCGGCTTCTTGCT+GGG + chr4_1:82172454-82172473 Msa0577860:intron 40.0%
CACCTTTACAGACACTACTA+AGG + chr4_1:82172583-82172602 Msa0577860:intron 40.0%
AGCCTTAGTAGTGTCTGTAA+AGG - chr4_1:82172588-82172607 None:intergenic 40.0%
GAAGATAGCTTGACATAGTC+TGG + chr4_1:82172775-82172794 Msa0577860:intron 40.0%
CATATCAAGCACACAAGAAG+CGG - chr4_1:82172928-82172947 None:intergenic 40.0%
CAATTTAGGCTCACAATGCT+TGG - chr4_1:82172997-82173016 None:intergenic 40.0%
!! TGGTCTTCATGATTTTCAGG+AGG + chr4_1:82173103-82173122 Msa0577860:intron 40.0%
!! GGTCTTCATGATTTTCAGGA+GGG + chr4_1:82173104-82173123 Msa0577860:intron 40.0%
! ATTTAGAGGGCAGGGATTTT+TGG - chr4_1:82173286-82173305 None:intergenic 40.0%
AGACACAGGAGTTTGTCAAT+CGG + chr4_1:82173333-82173352 Msa0577860:three_prime_UTR 40.0%
GAGTTTGTCAATCGGAATGT+TGG + chr4_1:82173341-82173360 Msa0577860:three_prime_UTR 40.0%
GAATGTTGGGATCACATACA+TGG + chr4_1:82173355-82173374 Msa0577860:three_prime_UTR 40.0%
GATCACATACATGGTTGGAT+AGG + chr4_1:82173364-82173383 Msa0577860:three_prime_UTR 40.0%
AATTGCAACCGTACGAAAGT+TGG + chr4_1:82173618-82173637 Msa0577860:three_prime_UTR 40.0%
AACCGTACGAAAGTTGGATT+TGG + chr4_1:82173624-82173643 Msa0577860:three_prime_UTR 40.0%
AAAAGTCCAGACAGAGCAGT+TGG + chr4_1:82170228-82170247 Msa0577860:five_prime_UTR 45.0%
!!! ACAGAGCAGTTGGTTTTCCT+GGG + chr4_1:82170238-82170257 Msa0577860:five_prime_UTR 45.0%
!!! CACGGACTAGGCTTTTGTTT+TGG + chr4_1:82170784-82170803 Msa0577860:intron 45.0%
!! ATCTTTAGGGTCTGGTTGGT+TGG + chr4_1:82170880-82170899 Msa0577860:intron 45.0%
! GGTTGGTTCAAGTTCAACAG+AGG + chr4_1:82170897-82170916 Msa0577860:intron 45.0%
! ATGGGTTTAGGGGGATTTTG+GGG + chr4_1:82170929-82170948 Msa0577860:intron 45.0%
AATCACGATCCTTGATGCTG+TGG + chr4_1:82171027-82171046 Msa0577860:intron 45.0%
CATGATTCTCCACAGCATCA+AGG - chr4_1:82171039-82171058 None:intergenic 45.0%
CTTGATGCTGTGGAGAATCA+TGG + chr4_1:82171037-82171056 Msa0577860:intron 45.0%
TGGAGAATCATGGTCAAACG+CGG + chr4_1:82171047-82171066 Msa0577860:intron 45.0%
GCGTAAATTCTGGCGATCAA+TGG - chr4_1:82171249-82171268 None:intergenic 45.0%
TAATTCGATGTTGACGCTGC+TGG - chr4_1:82171331-82171350 None:intergenic 45.0%
!! ATCGTGAAGCGGATTTTGTG+CGG - chr4_1:82171432-82171451 None:intergenic 45.0%
AACATCCCATAACAGTACCG+CGG + chr4_1:82171506-82171525 Msa0577860:intron 45.0%
CCACACCTATAAACCAGCAT+TGG - chr4_1:82171936-82171955 None:intergenic 45.0%
!! CCAATGCTGGTTTATAGGTG+TGG + chr4_1:82171933-82171952 Msa0577860:intron 45.0%
ACAGGAACCATGAACTGACA+AGG - chr4_1:82171960-82171979 None:intergenic 45.0%
GTTCATGGTTCCTGTACTTG+AGG + chr4_1:82171965-82171984 Msa0577860:CDS 45.0%
ATAGTCTGGACTCTGGTGTT+TGG + chr4_1:82172789-82172808 Msa0577860:intron 45.0%
! AGCAAGGTATTTAGAGGGCA+GGG - chr4_1:82173294-82173313 None:intergenic 45.0%
! TAGCAAGGTATTTAGAGGGC+AGG - chr4_1:82173295-82173314 None:intergenic 45.0%
GTTGGGATCACATACATGGT+TGG + chr4_1:82173359-82173378 Msa0577860:three_prime_UTR 45.0%
CACATACATGGTTGGATAGG+AGG + chr4_1:82173367-82173386 Msa0577860:three_prime_UTR 45.0%
CTGTGGTCAGGTTCAATTTG+TGG - chr4_1:82173665-82173684 None:intergenic 45.0%
CACAAATTGAACCTGACCAC+AGG + chr4_1:82173663-82173682 Msa0577860:three_prime_UTR 45.0%
!! AAGAATAATAATAATAATAA+TGG - chr4_1:82170620-82170639 None:intergenic 5.0%
GGAAAACCAACTGCTCTGTC+TGG - chr4_1:82170237-82170256 None:intergenic 50.0%
!!! GACAGAGCAGTTGGTTTTCC+TGG + chr4_1:82170237-82170256 Msa0577860:five_prime_UTR 50.0%
!! TGATGAAGGTACCAGACGTG+TGG - chr4_1:82170429-82170448 None:intergenic 50.0%
GGCGAGGAGTTCGAAATTGA+AGG - chr4_1:82170475-82170494 None:intergenic 50.0%
ACTCCTCGCCTTCTTCCAAT+TGG + chr4_1:82170485-82170504 Msa0577860:CDS 50.0%
GTACCAATTGGAAGAAGGCG+AGG - chr4_1:82170491-82170510 None:intergenic 50.0%
GGCGAGTACCAATTGGAAGA+AGG - chr4_1:82170496-82170515 None:intergenic 50.0%
GAAAGACGGCGAGTACCAAT+TGG - chr4_1:82170503-82170522 None:intergenic 50.0%
CGAGAGGAAGTAGGAAACGA+GGG - chr4_1:82170538-82170557 None:intergenic 50.0%
ACGAGAGGAAGTAGGAAACG+AGG - chr4_1:82170539-82170558 None:intergenic 50.0%
TCAACAGAGGGGAGCGAAAA+TGG + chr4_1:82170910-82170929 Msa0577860:intron 50.0%
CAACAGAGGGGAGCGAAAAT+GGG + chr4_1:82170911-82170930 Msa0577860:intron 50.0%
! AGGGGAGCGAAAATGGGTTT+AGG + chr4_1:82170917-82170936 Msa0577860:intron 50.0%
! GGGGAGCGAAAATGGGTTTA+GGG + chr4_1:82170918-82170937 Msa0577860:intron 50.0%
! GGGAGCGAAAATGGGTTTAG+GGG + chr4_1:82170919-82170938 Msa0577860:intron 50.0%
! GGAGCGAAAATGGGTTTAGG+GGG + chr4_1:82170920-82170939 Msa0577860:intron 50.0%
TTCTGCACCAATCCCCATCA+TGG + chr4_1:82171396-82171415 Msa0577860:intron 50.0%
! GTCTTTGCCATGATGGGGAT+TGG - chr4_1:82171406-82171425 None:intergenic 50.0%
GGATGGTCTTTGCCATGATG+GGG - chr4_1:82171411-82171430 None:intergenic 50.0%
CGGATGGTCTTTGCCATGAT+GGG - chr4_1:82171412-82171431 None:intergenic 50.0%
!! TGAAGCGGATTTTGTGCGGA+TGG - chr4_1:82171428-82171447 None:intergenic 50.0%
GCGGTACTGTTATGGGATGT+TGG - chr4_1:82171507-82171526 None:intergenic 50.0%
AGTATCAGAAGTAGAGGCCG+CGG - chr4_1:82171526-82171545 None:intergenic 50.0%
GTGTGGTCCTTGTCAGTTCA+TGG + chr4_1:82171950-82171969 Msa0577860:CDS 50.0%
TACTTGAGGAAGTGAGCGCT+CGG + chr4_1:82171979-82171998 Msa0577860:CDS 50.0%
GCTTGACATAGTCTGGACTC+TGG + chr4_1:82172782-82172801 Msa0577860:intron 50.0%
!! GGCAGGGATTTTTGGAGAGA+TGG - chr4_1:82173278-82173297 None:intergenic 50.0%
AGACATAAGCACCTGTGGTC+AGG - chr4_1:82173677-82173696 None:intergenic 50.0%
GACCACAGGTGCTTATGTCT+TGG + chr4_1:82173677-82173696 Msa0577860:three_prime_UTR 50.0%
TGCCAAGACATAAGCACCTG+TGG - chr4_1:82173682-82173701 None:intergenic 50.0%
AGACGCTGCTGAGATGGAAG+CGG - chr4_1:82170369-82170388 None:intergenic 55.0%
AGAGGAAGACGCTGCTGAGA+TGG - chr4_1:82170375-82170394 None:intergenic 55.0%
CTCTTCTTCGTCCACACGTC+TGG + chr4_1:82170415-82170434 Msa0577860:CDS 55.0%
AGGAAGTAGGAAACGAGGGC+GGG - chr4_1:82170534-82170553 None:intergenic 55.0%
GAGGAAGTAGGAAACGAGGG+CGG - chr4_1:82170535-82170554 None:intergenic 55.0%
GCGGATGGTCTTTGCCATGA+TGG - chr4_1:82171413-82171432 None:intergenic 55.0%
AGAGGCCGCGGTACTGTTAT+GGG - chr4_1:82171514-82171533 None:intergenic 55.0%
TAGAGGCCGCGGTACTGTTA+TGG - chr4_1:82171515-82171534 None:intergenic 55.0%
GCGCTCACTTCCTCAAGTAC+AGG - chr4_1:82171978-82171997 None:intergenic 55.0%
TGCTGAGATGGAAGCGGCCA+TGG - chr4_1:82170363-82170382 None:intergenic 60.0%
GGCGGGAGGAAGAGGAAAGA+CGG - chr4_1:82170517-82170536 None:intergenic 60.0%
AAGTAGGAAACGAGGGCGGG+AGG - chr4_1:82170531-82170550 None:intergenic 60.0%
CGCACGGGGAGAAAGTTACC+AGG - chr4_1:82171785-82171804 None:intergenic 60.0%
GAAACGAGGGCGGGAGGAAG+AGG - chr4_1:82170525-82170544 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 82170206 82173723 82170206 ID=Msa0577860;Name=Msa0577860
chr4_1 mRNA 82170206 82173723 82170206 ID=Msa0577860-mRNA-1;Parent=Msa0577860;Name=Msa0577860-mRNA-1;_AED=0.00;_eAED=0.00;_QI=156|1|1|1|0.75|0.6|5|404|175
chr4_1 exon 82170206 82170556 82170206 ID=Msa0577860-mRNA-1:exon:29658;Parent=Msa0577860-mRNA-1
chr4_1 exon 82171697 82171785 82171697 ID=Msa0577860-mRNA-1:exon:29659;Parent=Msa0577860-mRNA-1
chr4_1 exon 82171950 82172127 82171950 ID=Msa0577860-mRNA-1:exon:29660;Parent=Msa0577860-mRNA-1
chr4_1 exon 82173122 82173192 82173122 ID=Msa0577860-mRNA-1:exon:29661;Parent=Msa0577860-mRNA-1
chr4_1 exon 82173325 82173723 82173325 ID=Msa0577860-mRNA-1:exon:29662;Parent=Msa0577860-mRNA-1
chr4_1 five_prime_UTR 82170206 82170361 82170206 ID=Msa0577860-mRNA-1:five_prime_utr;Parent=Msa0577860-mRNA-1
chr4_1 CDS 82170362 82170556 82170362 ID=Msa0577860-mRNA-1:cds;Parent=Msa0577860-mRNA-1
chr4_1 CDS 82171697 82171785 82171697 ID=Msa0577860-mRNA-1:cds;Parent=Msa0577860-mRNA-1
chr4_1 CDS 82171950 82172127 82171950 ID=Msa0577860-mRNA-1:cds;Parent=Msa0577860-mRNA-1
chr4_1 CDS 82173122 82173187 82173122 ID=Msa0577860-mRNA-1:cds;Parent=Msa0577860-mRNA-1
chr4_1 three_prime_UTR 82173188 82173192 82173188 ID=Msa0577860-mRNA-1:three_prime_utr;Parent=Msa0577860-mRNA-1
chr4_1 three_prime_UTR 82173325 82173723 82173325 ID=Msa0577860-mRNA-1:three_prime_utr;Parent=Msa0577860-mRNA-1
Gene Sequence

>Msa0577860

ATGGCCGCTTCCATCTCAGCAGCGTCTTCCTCTGTAATCCTCCATTCTTTGAACTCTTCTTCGTCCACACGTCTGGTACCTTCATCATCATCATCATCATCATCTTCACACCTTCAATTTCGAACTCCTCGCCTTCTTCCAATTGGTACTCGCCGTCTTTCCTCTTCCTCCCGCCCTCGTTTCCTACTTCCTCTCGTTCAAGCAAAGAAACAAACCTTTTCCTCTTTTGATGATTTGCTTGCAAACTCCGACAAGCCTGTATTTGTTGACTTCTATGCAACCTGGTGTGGTCCTTGTCAGTTCATGGTTCCTGTACTTGAGGAAGTGAGCGCTCGGCTTCAAGATCAGATACAAATAGTTAAGATTGATACTGAGAAGTATCCTAGCATTGCCAACAAATACAACATTGAGGCATTGCCAACTTTCATCATCTTTAAGGATGGAAAACCCTTTGATCGCTTTGAGGGAGCATTAACAGCAGATAAGCTAATTGAACGCATAGAAACCACTCTCAACGTTAAGCAATAA
Protein sequence

>Msa0577860

MAASISAASSSVILHSLNSSSSTRLVPSSSSSSSSSHLQFRTPRLLPIGTRRLSSSSRPRFLLPLVQAKKQTFSSFDDLLANSDKPVFVDFYATWCGPCQFMVPVLEEVSARLQDQIQIVKIDTEKYPSIANKYNIEALPTFIIFKDGKPFDRFEGALTADKLIERIETTLNVKQ*