AlfalfaGEDB | Msa0579220

M. sativa cultivar zhongmu-4 / Msa0579220

KEGG: K02437
GO: GO:0000166 GO:0003674 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0008144 GO:0017076 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0097159 GO:0097367 GO:1901265 GO:1901363
PFAM: PF01597.22

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0579220	XP_003607890.1	98.065	155	3	0	1	155	1	155	1.12e-108	316

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0579220	sp\|O82179\|GCSH2_ARATH	78.205	156	33	1	1	155	1	156	2.76e-87	254

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0579220	Q2MGQ2	98.065	155	3	0	1	155	1	155	5.34e-109	316

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0022400	Msa0579220	0.841470	4.659602e-58	-8.615850e-47
Msa0033220	Msa0579220	0.856999	2.199518e-62	-8.615850e-47
Msa0044160	Msa0579220	0.819652	1.037803e-52	-8.615850e-47
Msa0044440	Msa0579220	0.838254	3.207251e-57	-8.615850e-47
Msa0044530	Msa0579220	-0.806982	6.313888e-50	-8.615850e-47
Msa0045810	Msa0579220	0.800725	1.259664e-48	-8.615850e-47
Msa0063940	Msa0579220	0.896789	2.633001e-76	-8.615850e-47
Msa0077340	Msa0579220	0.845506	3.892980e-59	-8.615850e-47
Msa0079880	Msa0579220	0.838777	2.351249e-57	-8.615850e-47
Msa0080030	Msa0579220	0.852543	4.308876e-61	-8.615850e-47
Msa0088520	Msa0579220	0.816402	5.634975e-52	-8.615850e-47
Msa0091240	Msa0579220	-0.803715	3.054187e-49	-8.615850e-47
Msa0098240	Msa0579220	0.841894	3.602157e-58	-8.615850e-47
Msa0101530	Msa0579220	0.852819	3.595041e-61	-8.615850e-47
Msa0105650	Msa0579220	0.840392	8.941461e-58	-8.615850e-47
Msa0124910	Msa0579220	0.805108	1.564988e-49	-8.615850e-47
Msa0125360	Msa0579220	0.819554	1.092970e-52	-8.615850e-47
Msa0127770	Msa0579220	0.800693	1.278708e-48	-8.615850e-47
Msa0139500	Msa0579220	0.805488	1.303104e-49	-8.615850e-47
Msa0142430	Msa0579220	0.850949	1.219296e-60	-8.615850e-47
Msa0161480	Msa0579220	0.824475	7.912646e-54	-8.615850e-47
Msa0165640	Msa0579220	0.856540	3.003547e-62	-8.615850e-47
Msa0169410	Msa0579220	0.804076	2.569082e-49	-8.615850e-47
Msa0183310	Msa0579220	-0.836784	7.636547e-57	-8.615850e-47
Msa0183710	Msa0579220	0.823119	1.644433e-53	-8.615850e-47
Msa0189270	Msa0579220	0.823077	1.682251e-53	-8.615850e-47
Msa0192250	Msa0579220	0.803794	2.941113e-49	-8.615850e-47
Msa0195230	Msa0579220	0.816851	4.467436e-52	-8.615850e-47
Msa0195260	Msa0579220	0.813690	2.253001e-51	-8.615850e-47
Msa0202490	Msa0579220	0.806207	9.199908e-50	-8.615850e-47
Msa0204950	Msa0579220	0.827608	1.424149e-54	-8.615850e-47
Msa0219280	Msa0579220	0.820597	6.305568e-53	-8.615850e-47
Msa0224110	Msa0579220	0.808518	2.977003e-50	-8.615850e-47
Msa0228490	Msa0579220	0.806157	9.427384e-50	-8.615850e-47
Msa0231630	Msa0579220	0.800431	1.445614e-48	-8.615850e-47
Msa0365210	Msa0579220	0.826950	2.047825e-54	-8.615850e-47
Msa0384590	Msa0579220	0.857482	1.584179e-62	-8.615850e-47
Msa0385930	Msa0579220	-0.802784	4.759627e-49	-8.615850e-47
Msa0388210	Msa0579220	0.800654	1.302006e-48	-8.615850e-47
Msa0397130	Msa0579220	0.807095	5.974614e-50	-8.615850e-47
Msa0402070	Msa0579220	-0.843361	1.468800e-58	-8.615850e-47
Msa0412790	Msa0579220	0.834168	3.503929e-56	-8.615850e-47
Msa0421220	Msa0579220	-0.805743	1.152183e-49	-8.615850e-47
Msa0424160	Msa0579220	0.804012	2.649455e-49	-8.615850e-47
Msa0434660	Msa0579220	0.843989	9.978683e-59	-8.615850e-47
Msa0436440	Msa0579220	0.806874	6.653053e-50	-8.615850e-47
Msa0437440	Msa0579220	-0.847665	1.001669e-59	-8.615850e-47
Msa0437520	Msa0579220	0.816368	5.734388e-52	-8.615850e-47
Msa0457560	Msa0579220	0.800977	1.118897e-48	-8.615850e-47
Msa0478430	Msa0579220	0.825157	5.462171e-54	-8.615850e-47
Msa0480900	Msa0579220	-0.818642	1.762118e-52	-8.615850e-47
Msa0256920	Msa0579220	0.806813	6.854543e-50	-8.615850e-47
Msa0276860	Msa0579220	0.805026	1.628236e-49	-8.615850e-47
Msa0281470	Msa0579220	0.861437	1.026599e-63	-8.615850e-47
Msa0286630	Msa0579220	0.811615	6.406814e-51	-8.615850e-47
Msa0288620	Msa0579220	0.812672	3.769007e-51	-8.615850e-47
Msa0298400	Msa0579220	0.802384	5.754899e-49	-8.615850e-47
Msa0313900	Msa0579220	0.816946	4.253911e-52	-8.615850e-47
Msa0319920	Msa0579220	0.811272	7.607982e-51	-8.615850e-47
Msa0320600	Msa0579220	0.819025	1.442320e-52	-8.615850e-47
Msa0330520	Msa0579220	0.826078	3.304780e-54	-8.615850e-47
Msa0336120	Msa0579220	0.804709	1.896795e-49	-8.615850e-47
Msa0354970	Msa0579220	-0.854336	1.317128e-61	-8.615850e-47
Msa0355080	Msa0579220	-0.882598	9.106073e-71	-8.615850e-47
Msa0484210	Msa0579220	-0.813672	2.273320e-51	-8.615850e-47
Msa0500650	Msa0579220	0.806298	8.804482e-50	-8.615850e-47
Msa0521610	Msa0579220	0.807186	5.716218e-50	-8.615850e-47
Msa0522540	Msa0579220	0.857125	2.019379e-62	-8.615850e-47
Msa0526150	Msa0579220	0.835799	1.359333e-56	-8.615850e-47
Msa0529030	Msa0579220	0.814647	1.384855e-51	-8.615850e-47
Msa0540670	Msa0579220	0.809330	1.996306e-50	-8.615850e-47
Msa0543560	Msa0579220	0.822993	1.759550e-53	-8.615850e-47
Msa0566280	Msa0579220	0.829267	5.663523e-55	-8.615850e-47
Msa0566340	Msa0579220	0.811867	5.647785e-51	-8.615850e-47
Msa0566940	Msa0579220	-0.831487	1.623362e-55	-8.615850e-47
Msa0567330	Msa0579220	0.806154	9.438902e-50	-8.615850e-47
Msa0576790	Msa0579220	0.824904	6.268444e-54	-8.615850e-47
Msa0577860	Msa0579220	-0.801312	9.555839e-49	-8.615850e-47
Msa0579080	Msa0579220	0.973278	3.696582e-136	-8.615850e-47
Msa0579160	Msa0579220	0.997067	2.263826e-236	-8.615850e-47
Msa0579220	Msa0582960	0.823090	1.669780e-53	-8.615850e-47
Msa0579220	Msa0590620	-0.828250	9.977534e-55	-8.615850e-47
Msa0579220	Msa0610690	0.969333	5.714777e-130	-8.615850e-47
Msa0579220	Msa0619780	0.838302	3.117331e-57	-8.615850e-47
Msa0579220	Msa0630440	0.819452	1.153007e-52	-8.615850e-47
Msa0579220	Msa0644330	0.809766	1.609105e-50	-8.615850e-47
Msa0579220	Msa0652770	0.814371	1.593945e-51	-8.615850e-47
Msa0579220	Msa0657710	0.831292	1.813674e-55	-8.615850e-47
Msa0579220	Msa0674480	0.856118	3.992001e-62	-8.615850e-47
Msa0579220	Msa0678380	0.820077	8.299643e-53	-8.615850e-47
Msa0579220	Msa0693330	0.821962	3.054518e-53	-8.615850e-47
Msa0579220	Msa0702800	0.842384	2.672013e-58	-8.615850e-47
Msa0579220	Msa0703950	0.836935	6.988417e-57	-8.615850e-47
Msa0579220	Msa0706230	0.814016	1.909406e-51	-8.615850e-47
Msa0579220	Msa0707360	0.826798	2.225759e-54	-8.615850e-47
Msa0579220	Msa0712860	0.852441	4.607954e-61	-8.615850e-47
Msa0579220	Msa0719750	0.804883	1.744584e-49	-8.615850e-47
Msa0579220	Msa0726170	-0.838771	2.359631e-57	-8.615850e-47
Msa0579220	Msa0733080	0.812798	3.536413e-51	-8.615850e-47
Msa0579220	Msa0736920	0.803619	3.197195e-49	-8.615850e-47
Msa0579220	Msa0742370	-0.872708	2.584028e-67	-8.615850e-47
Msa0579220	Msa0746650	0.826276	2.965540e-54	-8.615850e-47
Msa0579220	Msa0758780	-0.831714	1.427427e-55	-8.615850e-47
Msa0579220	Msa0758810	-0.835789	1.367226e-56	-8.615850e-47
Msa0579220	Msa0765960	-0.815628	8.386249e-52	-8.615850e-47
Msa0579220	Msa0774060	0.825494	4.546892e-54	-8.615850e-47
Msa0579220	Msa0779450	-0.839811	1.267412e-57	-8.615850e-47
Msa0579220	Msa0795750	0.896441	3.677759e-76	-8.615850e-47
Msa0579220	Msa0800480	0.850917	1.245321e-60	-8.615850e-47
Msa0579220	Msa0805390	0.829365	5.362100e-55	-8.615850e-47
Msa0579220	Msa0811690	0.881178	2.977656e-70	-8.615850e-47
Msa0579220	Msa0823480	-0.829301	5.558138e-55	-8.615850e-47
Msa0579220	Msa0828960	0.843052	1.775501e-58	-8.615850e-47
Msa0579220	Msa0831740	0.807812	4.210073e-50	-8.615850e-47
Msa0579220	Msa0833530	0.814847	1.250506e-51	-8.615850e-47
Msa0579220	Msa0838620	-0.816294	5.954770e-52	-8.615850e-47
Msa0579220	Msa0841050	0.803037	4.220901e-49	-8.615850e-47
Msa0579220	Msa0842140	0.825171	5.421288e-54	-8.615850e-47
Msa0579220	Msa0851850	0.905687	3.297964e-80	-8.615850e-47
Msa0579220	Msa0862480	-0.870151	1.811706e-66	-8.615850e-47
Msa0579220	Msa0865420	0.817362	3.430570e-52	-8.615850e-47
Msa0579220	Msa0894790	0.804353	2.249599e-49	-8.615850e-47
Msa0579220	Msa0909260	0.824877	6.361063e-54	-8.615850e-47
Msa0579220	Msa0928180	0.815820	7.600349e-52	-8.615850e-47
Msa0579220	Msa0930770	0.810055	1.394100e-50	-8.615850e-47
Msa0579220	Msa0940910	0.822302	2.547530e-53	-8.615850e-47
Msa0579220	Msa0945190	0.805265	1.450887e-49	-8.615850e-47
Msa0579220	Msa0958830	0.815465	9.115704e-52	-8.615850e-47
Msa0579220	Msa0964020	0.824944	6.132655e-54	-8.615850e-47
Msa0579220	Msa0967070	0.808680	2.750127e-50	-8.615850e-47
Msa0579220	Msa0967780	0.806376	8.476043e-50	-8.615850e-47
Msa0579220	Msa0972680	0.801066	1.072788e-48	-8.615850e-47
Msa0579220	Msa0982730	0.820885	5.414380e-53	-8.615850e-47
Msa0579220	Msa0993190	0.808846	2.533520e-50	-8.615850e-47
Msa0579220	Msa1015360	0.847031	1.495490e-59	-8.615850e-47
Msa0579220	Msa1022420	0.842605	2.335000e-58	-8.615850e-47
Msa0579220	Msa1028420	0.804055	2.595565e-49	-8.615850e-47
Msa0579220	Msa1031350	0.803434	3.492160e-49	-8.615850e-47
Msa0579220	Msa1032470	0.863552	2.294155e-64	-8.615850e-47
Msa0579220	Msa1039100	0.801448	8.962115e-49	-8.615850e-47
Msa0579220	Msa1039440	-0.829124	6.133438e-55	-8.615850e-47
Msa0579220	Msa1041060	0.803673	3.115652e-49	-8.615850e-47
Msa0579220	Msa1044250	0.847760	9.427801e-60	-8.615850e-47
Msa0579220	Msa1070460	0.806153	9.444667e-50	-8.615850e-47
Msa0579220	Msa1072250	0.816435	5.539040e-52	-8.615850e-47
Msa0579220	Msa1082410	0.828337	9.510605e-55	-8.615850e-47
Msa0579220	Msa1083700	0.807715	4.414679e-50	-8.615850e-47
Msa0579220	Msa1088400	-0.809024	2.321726e-50	-8.615850e-47
Msa0579220	Msa1094810	0.817220	3.691529e-52	-8.615850e-47
Msa0579220	Msa1102060	-0.811301	7.498661e-51	-8.615850e-47
Msa0579220	Msa1106590	0.816074	6.669623e-52	-8.615850e-47
Msa0579220	Msa1114690	0.861223	1.193062e-63	-8.615850e-47
Msa0579220	Msa1115600	0.807013	6.220490e-50	-8.615850e-47
Msa0579220	Msa1128520	-0.800338	1.510193e-48	-8.615850e-47
Msa0579220	Msa1133750	0.854217	1.426221e-61	-8.615850e-47
Msa0579220	Msa1146980	0.810956	8.908311e-51	-8.615850e-47
Msa0579220	Msa1147650	0.818755	1.660763e-52	-8.615850e-47
Msa0579220	Msa1154320	0.834196	3.446393e-56	-8.615850e-47
Msa0579220	Msa1155990	0.804388	2.212273e-49	-8.615850e-47
Msa0579220	Msa1165460	0.820901	5.368373e-53	-8.615850e-47
Msa0579220	Msa1166660	0.805751	1.147281e-49	-8.615850e-47
Msa0579220	Msa1184680	0.866710	2.337278e-65	-8.615850e-47
Msa0579220	Msa1190770	0.844527	7.156264e-59	-8.615850e-47
Msa0579220	Msa1194240	0.825863	3.716163e-54	-8.615850e-47
Msa0579220	Msa1197720	0.869087	4.027104e-66	-8.615850e-47
Msa0579220	Msa1203650	0.809571	1.771316e-50	-8.615850e-47
Msa0579220	Msa1207920	-0.848679	5.256748e-60	-8.615850e-47
Msa0579220	Msa1210040	0.822342	2.493048e-53	-8.615850e-47
Msa0579220	Msa1212830	0.832312	1.015642e-55	-8.615850e-47
Msa0579220	Msa1213040	0.840224	9.889982e-58	-8.615850e-47
Msa0579220	Msa1219930	0.858867	6.135936e-63	-8.615850e-47
Msa0579220	Msa1240190	0.817716	2.854657e-52	-8.615850e-47
Msa0579220	Msa1240220	0.811556	6.599482e-51	-8.615850e-47
Msa0579220	Msa1251410	0.879289	1.407555e-69	-8.615850e-47
Msa0579220	Msa1258630	0.800690	1.280618e-48	-8.615850e-47
Msa0579220	Msa1259160	0.816768	4.664220e-52	-8.615850e-47
Msa0579220	Msa1260220	0.867834	1.021761e-65	-8.615850e-47
Msa0579220	Msa1274260	0.802963	4.371492e-49	-8.615850e-47
Msa0579220	Msa1277110	0.802642	5.092246e-49	-8.615850e-47
Msa0579220	Msa1283930	0.813208	2.874802e-51	-8.615850e-47
Msa0579220	Msa1284020	0.803405	3.541951e-49	-8.615850e-47
Msa0579220	Msa1299650	0.823345	1.456232e-53	-8.615850e-47
Msa0579220	Msa1322960	0.816911	4.332337e-52	-8.615850e-47
Msa0579220	Msa1323070	0.859534	3.868962e-63	-8.615850e-47
Msa0579220	Msa1327800	0.824726	6.903996e-54	-8.615850e-47
Msa0579220	Msa1331570	-0.844507	7.241710e-59	-8.615850e-47
Msa0579220	Msa1343600	0.803348	3.638442e-49	-8.615850e-47
Msa0579220	Msa1349690	0.831069	2.056982e-55	-8.615850e-47
Msa0579220	Msa1351990	0.831356	1.748149e-55	-8.615850e-47
Msa0579220	Msa1352580	0.808937	2.422455e-50	-8.615850e-47
Msa0579220	Msa1352600	-0.812886	3.382542e-51	-8.615850e-47
Msa0579220	Msa1358290	0.825205	5.321865e-54	-8.615850e-47
Msa0579220	Msa1361980	0.808824	2.561719e-50	-8.615850e-47
Msa0579220	Msa1363010	0.818959	1.492634e-52	-8.615850e-47
Msa0579220	Msa1363280	-0.816681	4.878533e-52	-8.615850e-47
Msa0579220	Msa1368390	0.828587	8.276845e-55	-8.615850e-47
Msa0579220	Msa1372000	0.813779	2.152940e-51	-8.615850e-47
Msa0579220	Msa1381260	0.808909	2.456635e-50	-8.615850e-47
Msa0579220	Msa1384700	0.851210	1.029234e-60	-8.615850e-47
Msa0579220	Msa1387570	0.801385	9.232314e-49	-8.615850e-47
Msa0579220	Msa1389900	0.813880	2.046238e-51	-8.615850e-47
Msa0579220	Msa1391500	0.855425	6.362300e-62	-8.615850e-47
Msa0579220	Msa1392740	0.829234	5.769370e-55	-8.615850e-47
Msa0579220	Msa1396200	-0.868291	7.282316e-66	-8.615850e-47
Msa0579220	Msa1398810	0.806288	8.848077e-50	-8.615850e-47
Msa0579220	Msa1405460	0.818047	2.403388e-52	-8.615850e-47
Msa0579220	Msa1407840	0.830121	3.510597e-55	-8.615850e-47
Msa0579220	Msa1408390	0.809900	1.505205e-50	-8.615850e-47
Msa0579220	Msa1412000	0.823345	1.456347e-53	-8.615850e-47
Msa0579220	Msa1422860	0.875920	2.106341e-68	-8.615850e-47
Msa0579220	Msa1428230	-0.840563	8.065309e-58	-8.615850e-47
Msa0579220	Msa1433360	0.812647	3.815283e-51	-8.615850e-47
Msa0579220	Msa1434300	0.815749	7.882764e-52	-8.615850e-47
Msa0579220	Msa1437810	0.832652	8.364889e-56	-8.615850e-47
Msa0579220	Msa1439250	0.852837	3.552556e-61	-8.615850e-47
Msa0579220	Msa1439930	0.822127	2.796760e-53	-8.615850e-47
Msa0579220	Msa1445530	0.808436	3.099502e-50	-8.615850e-47
Msa0579220	Msa1447100	0.806351	8.578630e-50	-8.615850e-47
Msa0579220	Msa1454500	0.824659	7.160508e-54	-8.615850e-47
Msa0579220	Msa1457770	0.813691	2.251655e-51	-8.615850e-47
Msa0579220	Msa1457890	0.829069	6.326119e-55	-8.615850e-47
Msa0579220	Msa1461770	0.802381	5.763159e-49	-8.615850e-47
Msa0579220	Msa1464880	0.800185	1.622939e-48	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0579220	MtrunA17_Chr4g0043711	98.065	155	3	0	1	155	1	155	1.03e-112	316
Msa0579220	MtrunA17_Chr5g0400431	66.418	134	45	0	22	155	33	166	2.25e-66	199
Msa0579220	MtrunA17_Chr7g0260591	77.358	53	10	1	53	103	3	55	5.48e-20	78.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0579220	AT2G35120.1	78.205	156	33	1	1	155	1	156	2.81e-88	254
Msa0579220	AT1G32470.1	58.084	167	57	2	1	155	1	166	8.19e-63	191
Msa0579220	AT2G35370.1	65.441	136	47	0	20	155	30	165	1.88e-62	189

Find 58 sgRNAs with CRISPR-Local

Find 284 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GACATCAACTCTCCTGTTTC+AGG	0.209182	4_1:+83843874	Msa0579220:CDS
TTCACTGCCGTTCCCACTTC+TGG	0.241034	4_1:-83843805	None:intergenic
AGAAGTTCTATGAATTTAAT+AGG	0.244254	4_1:+83844488	Msa0579220:three_prime_UTR
AACTCAAGCCCCTATGAAAA+TGG	0.268193	4_1:+83844066	Msa0579220:CDS
AGATGGCTTGCAGGCAGTTA+TGG	0.326829	4_1:+83842355	Msa0579220:exon
AGTGAAGAGTGGTAGGGTTA+AGG	0.337032	4_1:-83842220	None:intergenic
TGATCATGCCCAAGATCATT+TGG	0.340864	4_1:+83843759	Msa0579220:CDS
CATCATGCAACTCATTTACT+AGG	0.362088	4_1:-83844614	None:intergenic
TGGTATAGATGATGTCAATT+TGG	0.362666	4_1:+83844224	Msa0579220:three_prime_UTR
GTGAAACAAGGAGATGGTTT+TGG	0.366878	4_1:+83843823	Msa0579220:CDS
ATCTTCTTCTTCACAGAACT+TGG	0.368009	4_1:-83844157	None:intergenic
CAACATCACCCAAATGATCT+TGG	0.387609	4_1:-83843768	None:intergenic
GATGGCTTGCAGGCAGTTAT+GGG	0.405390	4_1:+83842356	Msa0579220:exon
AGTGGTAGGGTTAAGGAAGT+TGG	0.419936	4_1:-83842213	None:intergenic
GAGAGAGAGTGAAGAGTGGT+AGG	0.424407	4_1:-83842227	None:intergenic
TGGCGAACTTGACAAATTGA+TGG	0.434808	4_1:+83844119	Msa0579220:CDS
GCTCAGCAACTCTCCTGCTT+TGG	0.435121	4_1:+83843921	Msa0579220:CDS
CCTGAGGTATGATGCGGCTC+TGG	0.441316	4_1:-83842382	None:intergenic
ATTCATTCATTCAGTGTAAG+TGG	0.444370	4_1:+83842301	Msa0579220:five_prime_UTR
TGAATGTCGTATGACAAAAC+TGG	0.478972	4_1:-83844427	None:intergenic
GATCATGCCCAAGATCATTT+GGG	0.482133	4_1:+83843760	Msa0579220:CDS
TGGGAAGTACTGAAAGATAT+CGG	0.488163	4_1:-83844200	None:intergenic
GCAATAAAACTCACCAAAGC+AGG	0.490891	4_1:-83843934	None:intergenic
CATGAATGGGTGAAAGTTGA+CGG	0.492157	4_1:+83843712	Msa0579220:CDS
AACATCACCCAAATGATCTT+GGG	0.497081	4_1:-83843767	None:intergenic
CCAGAGCCGCATCATACCTC+AGG	0.521213	4_1:+83842382	Msa0579220:CDS
AGTATGCTGATTCTCATGAA+TGG	0.523325	4_1:+83843698	Msa0579220:CDS
TGCGCTAGCTTCTGATGCAG+AGG	0.534256	4_1:+83844305	Msa0579220:three_prime_UTR
TTGACATCATCTATACCAAT+GGG	0.535199	4_1:-83844219	None:intergenic
CAAGCCCCTATGAAAATGGA+TGG	0.537218	4_1:+83844070	Msa0579220:CDS
ATCATGCAACTCATTTACTA+GGG	0.541363	4_1:-83844613	None:intergenic
TATCTTTCAGTACTTCCCAT+TGG	0.548271	4_1:+83844204	Msa0579220:three_prime_UTR
GCGCTAGCTTCTGATGCAGA+GGG	0.548373	4_1:+83844306	Msa0579220:three_prime_UTR
TTCCATCTTAGTTGAAAACA+AGG	0.549310	4_1:-83844464	None:intergenic
CTGAGAGAGAGAGTGAAGAG+TGG	0.551470	4_1:-83842231	None:intergenic
GTATGCTGATTCTCATGAAT+GGG	0.551627	4_1:+83843699	Msa0579220:CDS
AGAGAGAGTGAAGAGTGGTA+GGG	0.552288	4_1:-83842226	None:intergenic
ATTGACATCATCTATACCAA+TGG	0.555452	4_1:-83844220	None:intergenic
GACGGAAAATCTGCAACCAT+TGG	0.558598	4_1:+83843730	Msa0579220:CDS
TCGGCAAGACTATCAATGAC+TGG	0.570070	4_1:-83844181	None:intergenic
ATTGCTGTGGTTGGTGACAA+AGG	0.572500	4_1:-83842257	None:intergenic
TTTCACAGAACCTTCTCTAA+CGG	0.580128	4_1:+83842414	Msa0579220:CDS
TTGCTGTGGTTGGTGACAAA+GGG	0.582432	4_1:-83842256	None:intergenic
TCCATCTTAGTTGAAAACAA+GGG	0.618905	4_1:-83844463	None:intergenic
TGAATGAATACCGTTAGAGA+AGG	0.619094	4_1:-83842424	None:intergenic
TGGTGCAGTTGAAAGTGTGA+AGG	0.619953	4_1:+83843843	Msa0579220:CDS
TGAATTGCCAGAAGTGGGAA+CGG	0.630383	4_1:+83843798	Msa0579220:CDS
GTGGGAACGGCAGTGAAACA+AGG	0.631106	4_1:+83843811	Msa0579220:CDS
ACGGCAGTGAAACAAGGAGA+TGG	0.634473	4_1:+83843817	Msa0579220:CDS
GGCATGATCAGTTATGCCAA+TGG	0.640158	4_1:-83843746	None:intergenic
CATTCATTCAGTGTAAGTGG+TGG	0.640220	4_1:+83842304	Msa0579220:five_prime_UTR
GTATGTTGAATTGCCAGAAG+TGG	0.646780	4_1:+83843792	Msa0579220:CDS
TGTGAAAGACAGAGATCCTG+AGG	0.655630	4_1:-83842398	None:intergenic
TATGTTGAATTGCCAGAAGT+GGG	0.663199	4_1:+83843793	Msa0579220:CDS
TCATGCAACTCATTTACTAG+GGG	0.688705	4_1:-83844612	None:intergenic
AGAGATCCTGAGGTATGATG+CGG	0.690860	4_1:-83842388	None:intergenic
AAAGTTGAGATGAGTGACAG+TGG	0.702478	4_1:+83844099	Msa0579220:CDS
CATGCAACTCATTTACTAGG+GGG	0.728223	4_1:-83844611	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATAAAAGTTAATTATGAATA+AGG	-	chr4_1:83843959-83843978	None:intergenic	10.0%
!!	ATAAAAGTTAATTATGAATA+AGG	-	chr4_1:83843959-83843978	None:intergenic	10.0%
!!	TAATAATAAAATAAAAAGCG+AGG	-	chr4_1:83842529-83842548	None:intergenic	15.0%
!!	TTGTTAACAAAAAAACAAAA+TGG	-	chr4_1:83842759-83842778	None:intergenic	15.0%
!!!	AGATTTTATTGAGATATTAA+CGG	-	chr4_1:83843228-83843247	None:intergenic	15.0%
!!!	AACCAAAATTTATAATTTGT+AGG	+	chr4_1:83843376-83843395	Msa0579220:intron	15.0%
!!!	ATCCTACAAATTATAAATTT+TGG	-	chr4_1:83843381-83843400	None:intergenic	15.0%
!!!	AAAATTTATAATTTGTAGGA+TGG	+	chr4_1:83843380-83843399	Msa0579220:intron	15.0%
!!	TTAAAATTCAATATGCAAAT+TGG	-	chr4_1:83843426-83843445	None:intergenic	15.0%
!!!	TTTTAATATCTTGAAAGTTT+TGG	+	chr4_1:83843440-83843459	Msa0579220:intron	15.0%
!!	TAATAATAAAATAAAAAGCG+AGG	-	chr4_1:83842529-83842548	None:intergenic	15.0%
!!	TTGTTAACAAAAAAACAAAA+TGG	-	chr4_1:83842759-83842778	None:intergenic	15.0%
!!!	AGATTTTATTGAGATATTAA+CGG	-	chr4_1:83843228-83843247	None:intergenic	15.0%
!!!	AACCAAAATTTATAATTTGT+AGG	+	chr4_1:83843376-83843395	Msa0579220:intron	15.0%
!!!	ATCCTACAAATTATAAATTT+TGG	-	chr4_1:83843381-83843400	None:intergenic	15.0%
!!!	AAAATTTATAATTTGTAGGA+TGG	+	chr4_1:83843380-83843399	Msa0579220:intron	15.0%
!!	TTAAAATTCAATATGCAAAT+TGG	-	chr4_1:83843426-83843445	None:intergenic	15.0%
!!!	TTTTAATATCTTGAAAGTTT+TGG	+	chr4_1:83843440-83843459	Msa0579220:intron	15.0%
!!	AGATCAAGTTCAAAATAAAT+TGG	-	chr4_1:83842627-83842646	None:intergenic	20.0%
!!	ATATGTAAGTAACATATACT+AGG	+	chr4_1:83842778-83842797	Msa0579220:intron	20.0%
!!	AAAAATGTAACAAACAACAT+AGG	-	chr4_1:83842857-83842876	None:intergenic	20.0%
!!!	AAATGTTTGAATAGCTTTAT+AGG	-	chr4_1:83843127-83843146	None:intergenic	20.0%
!!!	ATTTTGTTCTAACTATTATC+TGG	+	chr4_1:83843260-83843279	Msa0579220:intron	20.0%
!!	AGAAGTTCTATGAATTTAAT+AGG	+	chr4_1:83844488-83844507	Msa0579220:three_prime_UTR	20.0%
!!	AAAGAAGAAAAAAACTAGTA+GGG	-	chr4_1:83844554-83844573	None:intergenic	20.0%
!!	AAAAGAAGAAAAAAACTAGT+AGG	-	chr4_1:83844555-83844574	None:intergenic	20.0%
!!	AGATCAAGTTCAAAATAAAT+TGG	-	chr4_1:83842627-83842646	None:intergenic	20.0%
!!	ATATGTAAGTAACATATACT+AGG	+	chr4_1:83842778-83842797	Msa0579220:intron	20.0%
!!	AAAAATGTAACAAACAACAT+AGG	-	chr4_1:83842857-83842876	None:intergenic	20.0%
!!!	AAATGTTTGAATAGCTTTAT+AGG	-	chr4_1:83843127-83843146	None:intergenic	20.0%
!!!	ATTTTGTTCTAACTATTATC+TGG	+	chr4_1:83843260-83843279	Msa0579220:intron	20.0%
!!	AGAAGTTCTATGAATTTAAT+AGG	+	chr4_1:83844488-83844507	Msa0579220:three_prime_UTR	20.0%
!!	AAAGAAGAAAAAAACTAGTA+GGG	-	chr4_1:83844554-83844573	None:intergenic	20.0%
!!	AAAAGAAGAAAAAAACTAGT+AGG	-	chr4_1:83844555-83844574	None:intergenic	20.0%
!	AACCACAGCAATATAAAAAA+GGG	+	chr4_1:83842268-83842287	Msa0579220:five_prime_UTR	25.0%
!	TCAAGTTCAAAATAAATTGG+AGG	-	chr4_1:83842624-83842643	None:intergenic	25.0%
!!	TTTTAGTGAATAGTGACAAA+AGG	+	chr4_1:83842727-83842746	Msa0579220:intron	25.0%
!!	AGAATATTCCATTTCCATTT+TGG	-	chr4_1:83842811-83842830	None:intergenic	25.0%
!!!	AAGTTCGTTTTTGCTTTTAA+AGG	+	chr4_1:83842976-83842995	Msa0579220:intron	25.0%
!	ATAACTCAGTCAAAAGAAAT+TGG	-	chr4_1:83843091-83843110	None:intergenic	25.0%
!!!	GAATAGCTTTATAGGTAAAT+TGG	-	chr4_1:83843119-83843138	None:intergenic	25.0%
!!	TAACGGTTTAATTGATTTCA+AGG	-	chr4_1:83843211-83843230	None:intergenic	25.0%
!	TATTATCTGGTACATCATAT+TGG	+	chr4_1:83843273-83843292	Msa0579220:intron	25.0%
!	TTAAAACTTAAAGTGAACGA+TGG	+	chr4_1:83843307-83843326	Msa0579220:intron	25.0%
!	TAAAACTTAAAGTGAACGAT+GGG	+	chr4_1:83843308-83843327	Msa0579220:intron	25.0%
!	CTTACAAATGAACAATGATA+TGG	-	chr4_1:83843339-83843358	None:intergenic	25.0%
!	TAGGAAAACATAAAAACACT+TGG	-	chr4_1:83844028-83844047	None:intergenic	25.0%
!!	ATTATCCATCCATTTTCATA+GGG	-	chr4_1:83844078-83844097	None:intergenic	25.0%
!!	AATTATCCATCCATTTTCAT+AGG	-	chr4_1:83844079-83844098	None:intergenic	25.0%
!!	AAAAATGAGATTGATAACCT+GGG	+	chr4_1:83844354-83844373	Msa0579220:three_prime_UTR	25.0%
!!	TTTTTATTATCAAACTTCCC+AGG	-	chr4_1:83844374-83844393	None:intergenic	25.0%
!	AACCACAGCAATATAAAAAA+GGG	+	chr4_1:83842268-83842287	Msa0579220:five_prime_UTR	25.0%
!	TCAAGTTCAAAATAAATTGG+AGG	-	chr4_1:83842624-83842643	None:intergenic	25.0%
!!	TTTTAGTGAATAGTGACAAA+AGG	+	chr4_1:83842727-83842746	Msa0579220:intron	25.0%
!!	AGAATATTCCATTTCCATTT+TGG	-	chr4_1:83842811-83842830	None:intergenic	25.0%
!!!	AAGTTCGTTTTTGCTTTTAA+AGG	+	chr4_1:83842976-83842995	Msa0579220:intron	25.0%
!	ATAACTCAGTCAAAAGAAAT+TGG	-	chr4_1:83843091-83843110	None:intergenic	25.0%
!!!	GAATAGCTTTATAGGTAAAT+TGG	-	chr4_1:83843119-83843138	None:intergenic	25.0%
!!	TAACGGTTTAATTGATTTCA+AGG	-	chr4_1:83843211-83843230	None:intergenic	25.0%
!	TATTATCTGGTACATCATAT+TGG	+	chr4_1:83843273-83843292	Msa0579220:intron	25.0%
!	TTAAAACTTAAAGTGAACGA+TGG	+	chr4_1:83843307-83843326	Msa0579220:intron	25.0%
!	TAAAACTTAAAGTGAACGAT+GGG	+	chr4_1:83843308-83843327	Msa0579220:intron	25.0%
!	CTTACAAATGAACAATGATA+TGG	-	chr4_1:83843339-83843358	None:intergenic	25.0%
!	TAGGAAAACATAAAAACACT+TGG	-	chr4_1:83844028-83844047	None:intergenic	25.0%
!!	ATTATCCATCCATTTTCATA+GGG	-	chr4_1:83844078-83844097	None:intergenic	25.0%
!!	AATTATCCATCCATTTTCAT+AGG	-	chr4_1:83844079-83844098	None:intergenic	25.0%
!!	AAAAATGAGATTGATAACCT+GGG	+	chr4_1:83844354-83844373	Msa0579220:three_prime_UTR	25.0%
!!	TTTTTATTATCAAACTTCCC+AGG	-	chr4_1:83844374-83844393	None:intergenic	25.0%
!!!	CTTTTTTATATTGCTGTGGT+TGG	-	chr4_1:83842269-83842288	None:intergenic	30.0%
	CAACCACAGCAATATAAAAA+AGG	+	chr4_1:83842267-83842286	Msa0579220:five_prime_UTR	30.0%
	ATTCATTCATTCAGTGTAAG+TGG	+	chr4_1:83842301-83842320	Msa0579220:five_prime_UTR	30.0%
	ATTGCCTTTATATACGTTAG+TGG	+	chr4_1:83842564-83842583	Msa0579220:intron	30.0%
	TTGCCTTTATATACGTTAGT+GGG	+	chr4_1:83842565-83842584	Msa0579220:intron	30.0%
!	TTTTGAACTTGATCTGTATG+TGG	+	chr4_1:83842632-83842651	Msa0579220:intron	30.0%
	AATTGAAAGAATAGATCGTC+CGG	-	chr4_1:83842709-83842728	None:intergenic	30.0%
	TACTAGGCTAAATTCCAAAA+TGG	+	chr4_1:83842794-83842813	Msa0579220:intron	30.0%
	GCTAAATTCCAAAATGGAAA+TGG	+	chr4_1:83842800-83842819	Msa0579220:intron	30.0%
	GTGCATATTTCGAAATTAAG+TGG	+	chr4_1:83842934-83842953	Msa0579220:intron	30.0%
!!	AATTTAGCTTGTTAGCACTT+TGG	+	chr4_1:83843013-83843032	Msa0579220:intron	30.0%
!!	ATTTAGCTTGTTAGCACTTT+GGG	+	chr4_1:83843014-83843033	Msa0579220:intron	30.0%
!!	TAACAAACTGTTGGTTTGTT+TGG	+	chr4_1:83843043-83843062	Msa0579220:intron	30.0%
	AATAATCCAAATTACTCTCG+AGG	+	chr4_1:83843150-83843169	Msa0579220:intron	30.0%
!	CAATATGCTACTTGTTCAAT+TGG	-	chr4_1:83843578-83843597	None:intergenic	30.0%
	CAATTGAACAAGTAGCATAT+TGG	+	chr4_1:83843576-83843595	Msa0579220:intron	30.0%
!	ATTGAAACCAACATTTTGCA+AGG	+	chr4_1:83843626-83843645	Msa0579220:intron	30.0%
!!	TTTCCTATTTTTCTTCGTGT+AGG	+	chr4_1:83844041-83844060	Msa0579220:intron	30.0%
!	TTATCCATCCATTTTCATAG+GGG	-	chr4_1:83844077-83844096	None:intergenic	30.0%
	TTGACATCATCTATACCAAT+GGG	-	chr4_1:83844222-83844241	None:intergenic	30.0%
	ATTGACATCATCTATACCAA+TGG	-	chr4_1:83844223-83844242	None:intergenic	30.0%
	TGGTATAGATGATGTCAATT+TGG	+	chr4_1:83844224-83844243	Msa0579220:three_prime_UTR	30.0%
!!	TCTCATTTTTCCATTGCAAA+GGG	-	chr4_1:83844344-83844363	None:intergenic	30.0%
!!	ATCTCATTTTTCCATTGCAA+AGG	-	chr4_1:83844345-83844364	None:intergenic	30.0%
!	GAAAAATGAGATTGATAACC+TGG	+	chr4_1:83844353-83844372	Msa0579220:three_prime_UTR	30.0%
	TCCATCTTAGTTGAAAACAA+GGG	-	chr4_1:83844466-83844485	None:intergenic	30.0%
	TTCCATCTTAGTTGAAAACA+AGG	-	chr4_1:83844467-83844486	None:intergenic	30.0%
!!	AAAAACTAGTAGGGTTTTAG+TGG	-	chr4_1:83844545-83844564	None:intergenic	30.0%
	ATCATGCAACTCATTTACTA+GGG	-	chr4_1:83844616-83844635	None:intergenic	30.0%
!!!	CTTTTTTATATTGCTGTGGT+TGG	-	chr4_1:83842269-83842288	None:intergenic	30.0%
	CAACCACAGCAATATAAAAA+AGG	+	chr4_1:83842267-83842286	Msa0579220:five_prime_UTR	30.0%
	ATTCATTCATTCAGTGTAAG+TGG	+	chr4_1:83842301-83842320	Msa0579220:five_prime_UTR	30.0%
	ATTGCCTTTATATACGTTAG+TGG	+	chr4_1:83842564-83842583	Msa0579220:intron	30.0%
	TTGCCTTTATATACGTTAGT+GGG	+	chr4_1:83842565-83842584	Msa0579220:intron	30.0%
!	TTTTGAACTTGATCTGTATG+TGG	+	chr4_1:83842632-83842651	Msa0579220:intron	30.0%
	AATTGAAAGAATAGATCGTC+CGG	-	chr4_1:83842709-83842728	None:intergenic	30.0%
	TACTAGGCTAAATTCCAAAA+TGG	+	chr4_1:83842794-83842813	Msa0579220:intron	30.0%
	GCTAAATTCCAAAATGGAAA+TGG	+	chr4_1:83842800-83842819	Msa0579220:intron	30.0%
	GTGCATATTTCGAAATTAAG+TGG	+	chr4_1:83842934-83842953	Msa0579220:intron	30.0%
!!	AATTTAGCTTGTTAGCACTT+TGG	+	chr4_1:83843013-83843032	Msa0579220:intron	30.0%
!!	ATTTAGCTTGTTAGCACTTT+GGG	+	chr4_1:83843014-83843033	Msa0579220:intron	30.0%
!!	TAACAAACTGTTGGTTTGTT+TGG	+	chr4_1:83843043-83843062	Msa0579220:intron	30.0%
	AATAATCCAAATTACTCTCG+AGG	+	chr4_1:83843150-83843169	Msa0579220:intron	30.0%
!	CAATATGCTACTTGTTCAAT+TGG	-	chr4_1:83843578-83843597	None:intergenic	30.0%
	CAATTGAACAAGTAGCATAT+TGG	+	chr4_1:83843576-83843595	Msa0579220:intron	30.0%
!	ATTGAAACCAACATTTTGCA+AGG	+	chr4_1:83843626-83843645	Msa0579220:intron	30.0%
!!	TTTCCTATTTTTCTTCGTGT+AGG	+	chr4_1:83844041-83844060	Msa0579220:intron	30.0%
!	TTATCCATCCATTTTCATAG+GGG	-	chr4_1:83844077-83844096	None:intergenic	30.0%
	TTGACATCATCTATACCAAT+GGG	-	chr4_1:83844222-83844241	None:intergenic	30.0%
	ATTGACATCATCTATACCAA+TGG	-	chr4_1:83844223-83844242	None:intergenic	30.0%
	TGGTATAGATGATGTCAATT+TGG	+	chr4_1:83844224-83844243	Msa0579220:three_prime_UTR	30.0%
!!	TCTCATTTTTCCATTGCAAA+GGG	-	chr4_1:83844344-83844363	None:intergenic	30.0%
!!	ATCTCATTTTTCCATTGCAA+AGG	-	chr4_1:83844345-83844364	None:intergenic	30.0%
!	GAAAAATGAGATTGATAACC+TGG	+	chr4_1:83844353-83844372	Msa0579220:three_prime_UTR	30.0%
	TCCATCTTAGTTGAAAACAA+GGG	-	chr4_1:83844466-83844485	None:intergenic	30.0%
	TTCCATCTTAGTTGAAAACA+AGG	-	chr4_1:83844467-83844486	None:intergenic	30.0%
!!	AAAAACTAGTAGGGTTTTAG+TGG	-	chr4_1:83844545-83844564	None:intergenic	30.0%
	ATCATGCAACTCATTTACTA+GGG	-	chr4_1:83844616-83844635	None:intergenic	30.0%
!!!	TGCCCTTTTTTATATTGCTG+TGG	-	chr4_1:83842273-83842292	None:intergenic	35.0%
	TTTCACAGAACCTTCTCTAA+CGG	+	chr4_1:83842414-83842433	Msa0579220:CDS	35.0%
	TGAATGAATACCGTTAGAGA+AGG	-	chr4_1:83842427-83842446	None:intergenic	35.0%
	CATGTTGTTGATCCAATATC+TGG	+	chr4_1:83842488-83842507	Msa0579220:intron	35.0%
!	TTGATCCAATATCTGGAATC+TGG	+	chr4_1:83842495-83842514	Msa0579220:intron	35.0%
	AAGATCCAGATTCCAGATAT+TGG	-	chr4_1:83842503-83842522	None:intergenic	35.0%
	CTTCCCACTAACGTATATAA+AGG	-	chr4_1:83842571-83842590	None:intergenic	35.0%
	CTTTATATACGTTAGTGGGA+AGG	+	chr4_1:83842569-83842588	Msa0579220:intron	35.0%
	ATAAATTGGAGGTGACTGAA+TGG	-	chr4_1:83842613-83842632	None:intergenic	35.0%
!!	TTTAGCTTGTTAGCACTTTG+GGG	+	chr4_1:83843015-83843034	Msa0579220:intron	35.0%
	ATGTTTCCTCGAGAGTAATT+TGG	-	chr4_1:83843159-83843178	None:intergenic	35.0%
!	CGGTTTAATTGATTTCAAGG+AGG	-	chr4_1:83843208-83843227	None:intergenic	35.0%
	ACATGATAATCATCATCTGC+AGG	+	chr4_1:83843499-83843518	Msa0579220:intron	35.0%
	CATACAACCTTGCAAAATGT+TGG	-	chr4_1:83843636-83843655	None:intergenic	35.0%
!!	AGTATGCTGATTCTCATGAA+TGG	+	chr4_1:83843698-83843717	Msa0579220:CDS	35.0%
!!	GTATGCTGATTCTCATGAAT+GGG	+	chr4_1:83843699-83843718	Msa0579220:CDS	35.0%
	AACATCACCCAAATGATCTT+GGG	-	chr4_1:83843770-83843789	None:intergenic	35.0%
	TATGTTGAATTGCCAGAAGT+GGG	+	chr4_1:83843793-83843812	Msa0579220:CDS	35.0%
!!	TCAATCACTTTTCCTGAAAC+AGG	-	chr4_1:83843889-83843908	None:intergenic	35.0%
	TGACCTACACGAAGAAAAAT+AGG	-	chr4_1:83844047-83844066	None:intergenic	35.0%
	ATCTTCTTCTTCACAGAACT+TGG	-	chr4_1:83844160-83844179	None:intergenic	35.0%
!!	TGGGAAGTACTGAAAGATAT+CGG	-	chr4_1:83844203-83844222	None:intergenic	35.0%
	TATCTTTCAGTACTTCCCAT+TGG	+	chr4_1:83844204-83844223	Msa0579220:three_prime_UTR	35.0%
!!	TCATTTTTCCATTGCAAAGG+GGG	-	chr4_1:83844342-83844361	None:intergenic	35.0%
!!	CTCATTTTTCCATTGCAAAG+GGG	-	chr4_1:83844343-83844362	None:intergenic	35.0%
	TGAATGTCGTATGACAAAAC+TGG	-	chr4_1:83844430-83844449	None:intergenic	35.0%
	TCATGCAACTCATTTACTAG+GGG	-	chr4_1:83844615-83844634	None:intergenic	35.0%
	CATCATGCAACTCATTTACT+AGG	-	chr4_1:83844617-83844636	None:intergenic	35.0%
!!!	TGCCCTTTTTTATATTGCTG+TGG	-	chr4_1:83842273-83842292	None:intergenic	35.0%
	TTTCACAGAACCTTCTCTAA+CGG	+	chr4_1:83842414-83842433	Msa0579220:CDS	35.0%
	TGAATGAATACCGTTAGAGA+AGG	-	chr4_1:83842427-83842446	None:intergenic	35.0%
	CATGTTGTTGATCCAATATC+TGG	+	chr4_1:83842488-83842507	Msa0579220:intron	35.0%
!	TTGATCCAATATCTGGAATC+TGG	+	chr4_1:83842495-83842514	Msa0579220:intron	35.0%
	AAGATCCAGATTCCAGATAT+TGG	-	chr4_1:83842503-83842522	None:intergenic	35.0%
	CTTCCCACTAACGTATATAA+AGG	-	chr4_1:83842571-83842590	None:intergenic	35.0%
	CTTTATATACGTTAGTGGGA+AGG	+	chr4_1:83842569-83842588	Msa0579220:intron	35.0%
	ATAAATTGGAGGTGACTGAA+TGG	-	chr4_1:83842613-83842632	None:intergenic	35.0%
!!	TTTAGCTTGTTAGCACTTTG+GGG	+	chr4_1:83843015-83843034	Msa0579220:intron	35.0%
	ATGTTTCCTCGAGAGTAATT+TGG	-	chr4_1:83843159-83843178	None:intergenic	35.0%
!	CGGTTTAATTGATTTCAAGG+AGG	-	chr4_1:83843208-83843227	None:intergenic	35.0%
	ACATGATAATCATCATCTGC+AGG	+	chr4_1:83843499-83843518	Msa0579220:intron	35.0%
	CATACAACCTTGCAAAATGT+TGG	-	chr4_1:83843636-83843655	None:intergenic	35.0%
!!	AGTATGCTGATTCTCATGAA+TGG	+	chr4_1:83843698-83843717	Msa0579220:CDS	35.0%
!!	GTATGCTGATTCTCATGAAT+GGG	+	chr4_1:83843699-83843718	Msa0579220:CDS	35.0%
	AACATCACCCAAATGATCTT+GGG	-	chr4_1:83843770-83843789	None:intergenic	35.0%
	TATGTTGAATTGCCAGAAGT+GGG	+	chr4_1:83843793-83843812	Msa0579220:CDS	35.0%
!!	TCAATCACTTTTCCTGAAAC+AGG	-	chr4_1:83843889-83843908	None:intergenic	35.0%
	TGACCTACACGAAGAAAAAT+AGG	-	chr4_1:83844047-83844066	None:intergenic	35.0%
	ATCTTCTTCTTCACAGAACT+TGG	-	chr4_1:83844160-83844179	None:intergenic	35.0%
!!	TGGGAAGTACTGAAAGATAT+CGG	-	chr4_1:83844203-83844222	None:intergenic	35.0%
	TATCTTTCAGTACTTCCCAT+TGG	+	chr4_1:83844204-83844223	Msa0579220:three_prime_UTR	35.0%
!!	TCATTTTTCCATTGCAAAGG+GGG	-	chr4_1:83844342-83844361	None:intergenic	35.0%
!!	CTCATTTTTCCATTGCAAAG+GGG	-	chr4_1:83844343-83844362	None:intergenic	35.0%
	TGAATGTCGTATGACAAAAC+TGG	-	chr4_1:83844430-83844449	None:intergenic	35.0%
	TCATGCAACTCATTTACTAG+GGG	-	chr4_1:83844615-83844634	None:intergenic	35.0%
	CATCATGCAACTCATTTACT+AGG	-	chr4_1:83844617-83844636	None:intergenic	35.0%
	CATTCATTCAGTGTAAGTGG+TGG	+	chr4_1:83842304-83842323	Msa0579220:five_prime_UTR	40.0%
	TGAATGGTGTAGTGCACTTT+CGG	-	chr4_1:83842597-83842616	None:intergenic	40.0%
!!	CACCAAAAGTTGATATACGG+CGG	-	chr4_1:83842686-83842705	None:intergenic	40.0%
!!	CGGCACCAAAAGTTGATATA+CGG	-	chr4_1:83842689-83842708	None:intergenic	40.0%
!!	CGTATATCAACTTTTGGTGC+CGG	+	chr4_1:83842687-83842706	Msa0579220:intron	40.0%
!!	TTAGCTTGTTAGCACTTTGG+GGG	+	chr4_1:83843016-83843035	Msa0579220:intron	40.0%
	GGGGGAAAATAACAAACTGT+TGG	+	chr4_1:83843034-83843053	Msa0579220:intron	40.0%
	CATGAATGGGTGAAAGTTGA+CGG	+	chr4_1:83843712-83843731	Msa0579220:CDS	40.0%
	TGATCATGCCCAAGATCATT+TGG	+	chr4_1:83843759-83843778	Msa0579220:CDS	40.0%
	GATCATGCCCAAGATCATTT+GGG	+	chr4_1:83843760-83843779	Msa0579220:CDS	40.0%
	CAACATCACCCAAATGATCT+TGG	-	chr4_1:83843771-83843790	None:intergenic	40.0%
	GTATGTTGAATTGCCAGAAG+TGG	+	chr4_1:83843792-83843811	Msa0579220:CDS	40.0%
!	GTGAAACAAGGAGATGGTTT+TGG	+	chr4_1:83843823-83843842	Msa0579220:CDS	40.0%
	GCAATAAAACTCACCAAAGC+AGG	-	chr4_1:83843937-83843956	None:intergenic	40.0%
	AACTCAAGCCCCTATGAAAA+TGG	+	chr4_1:83844066-83844085	Msa0579220:CDS	40.0%
	AAAGTTGAGATGAGTGACAG+TGG	+	chr4_1:83844099-83844118	Msa0579220:CDS	40.0%
	TGGCGAACTTGACAAATTGA+TGG	+	chr4_1:83844119-83844138	Msa0579220:CDS	40.0%
!	GATATTTTCCCCCTTTGCAA+TGG	+	chr4_1:83844331-83844350	Msa0579220:three_prime_UTR	40.0%
!!!	GCCCTTGTTTTCAACTAAGA+TGG	+	chr4_1:83844462-83844481	Msa0579220:three_prime_UTR	40.0%
	CATGCAACTCATTTACTAGG+GGG	-	chr4_1:83844614-83844633	None:intergenic	40.0%
	CATTCATTCAGTGTAAGTGG+TGG	+	chr4_1:83842304-83842323	Msa0579220:five_prime_UTR	40.0%
	TGAATGGTGTAGTGCACTTT+CGG	-	chr4_1:83842597-83842616	None:intergenic	40.0%
!!	CACCAAAAGTTGATATACGG+CGG	-	chr4_1:83842686-83842705	None:intergenic	40.0%
!!	CGGCACCAAAAGTTGATATA+CGG	-	chr4_1:83842689-83842708	None:intergenic	40.0%
!!	CGTATATCAACTTTTGGTGC+CGG	+	chr4_1:83842687-83842706	Msa0579220:intron	40.0%
!!	TTAGCTTGTTAGCACTTTGG+GGG	+	chr4_1:83843016-83843035	Msa0579220:intron	40.0%
	GGGGGAAAATAACAAACTGT+TGG	+	chr4_1:83843034-83843053	Msa0579220:intron	40.0%
	CATGAATGGGTGAAAGTTGA+CGG	+	chr4_1:83843712-83843731	Msa0579220:CDS	40.0%
	TGATCATGCCCAAGATCATT+TGG	+	chr4_1:83843759-83843778	Msa0579220:CDS	40.0%
	GATCATGCCCAAGATCATTT+GGG	+	chr4_1:83843760-83843779	Msa0579220:CDS	40.0%
	CAACATCACCCAAATGATCT+TGG	-	chr4_1:83843771-83843790	None:intergenic	40.0%
	GTATGTTGAATTGCCAGAAG+TGG	+	chr4_1:83843792-83843811	Msa0579220:CDS	40.0%
!	GTGAAACAAGGAGATGGTTT+TGG	+	chr4_1:83843823-83843842	Msa0579220:CDS	40.0%
	GCAATAAAACTCACCAAAGC+AGG	-	chr4_1:83843937-83843956	None:intergenic	40.0%
	AACTCAAGCCCCTATGAAAA+TGG	+	chr4_1:83844066-83844085	Msa0579220:CDS	40.0%
	AAAGTTGAGATGAGTGACAG+TGG	+	chr4_1:83844099-83844118	Msa0579220:CDS	40.0%
	TGGCGAACTTGACAAATTGA+TGG	+	chr4_1:83844119-83844138	Msa0579220:CDS	40.0%
!	GATATTTTCCCCCTTTGCAA+TGG	+	chr4_1:83844331-83844350	Msa0579220:three_prime_UTR	40.0%
!!!	GCCCTTGTTTTCAACTAAGA+TGG	+	chr4_1:83844462-83844481	Msa0579220:three_prime_UTR	40.0%
	CATGCAACTCATTTACTAGG+GGG	-	chr4_1:83844614-83844633	None:intergenic	40.0%
	AGTGGTAGGGTTAAGGAAGT+TGG	-	chr4_1:83842216-83842235	None:intergenic	45.0%
	AGTGAAGAGTGGTAGGGTTA+AGG	-	chr4_1:83842223-83842242	None:intergenic	45.0%
	AGAGAGAGTGAAGAGTGGTA+GGG	-	chr4_1:83842229-83842248	None:intergenic	45.0%
!!	TTGCTGTGGTTGGTGACAAA+GGG	-	chr4_1:83842259-83842278	None:intergenic	45.0%
!!	ATTGCTGTGGTTGGTGACAA+AGG	-	chr4_1:83842260-83842279	None:intergenic	45.0%
	ACCGCAGACAGAGAAAAAGA+TGG	+	chr4_1:83842338-83842357	Msa0579220:exon	45.0%
!	GCCATCTTTTTCTCTGTCTG+CGG	-	chr4_1:83842342-83842361	None:intergenic	45.0%
!	CAGAGAAAAAGATGGCTTGC+AGG	+	chr4_1:83842346-83842365	Msa0579220:exon	45.0%
	AGAGATCCTGAGGTATGATG+CGG	-	chr4_1:83842391-83842410	None:intergenic	45.0%
	TGTGAAAGACAGAGATCCTG+AGG	-	chr4_1:83842401-83842420	None:intergenic	45.0%
!	GTCCGCCGTATATCAACTTT+TGG	+	chr4_1:83842681-83842700	Msa0579220:intron	45.0%
	CAGATGAGAAGACAGAGACT+CGG	-	chr4_1:83843473-83843492	None:intergenic	45.0%
	GACGGAAAATCTGCAACCAT+TGG	+	chr4_1:83843730-83843749	Msa0579220:CDS	45.0%
	GGCATGATCAGTTATGCCAA+TGG	-	chr4_1:83843749-83843768	None:intergenic	45.0%
	TGAATTGCCAGAAGTGGGAA+CGG	+	chr4_1:83843798-83843817	Msa0579220:CDS	45.0%
!	TGGTGCAGTTGAAAGTGTGA+AGG	+	chr4_1:83843843-83843862	Msa0579220:CDS	45.0%
	GACATCAACTCTCCTGTTTC+AGG	+	chr4_1:83843874-83843893	Msa0579220:CDS	45.0%
	CAAGCCCCTATGAAAATGGA+TGG	+	chr4_1:83844070-83844089	Msa0579220:CDS	45.0%
	TCGGCAAGACTATCAATGAC+TGG	-	chr4_1:83844184-83844203	None:intergenic	45.0%
	AGTGGTAGGGTTAAGGAAGT+TGG	-	chr4_1:83842216-83842235	None:intergenic	45.0%
	AGTGAAGAGTGGTAGGGTTA+AGG	-	chr4_1:83842223-83842242	None:intergenic	45.0%
	AGAGAGAGTGAAGAGTGGTA+GGG	-	chr4_1:83842229-83842248	None:intergenic	45.0%
!!	TTGCTGTGGTTGGTGACAAA+GGG	-	chr4_1:83842259-83842278	None:intergenic	45.0%
!!	ATTGCTGTGGTTGGTGACAA+AGG	-	chr4_1:83842260-83842279	None:intergenic	45.0%
	ACCGCAGACAGAGAAAAAGA+TGG	+	chr4_1:83842338-83842357	Msa0579220:exon	45.0%
!	GCCATCTTTTTCTCTGTCTG+CGG	-	chr4_1:83842342-83842361	None:intergenic	45.0%
!	CAGAGAAAAAGATGGCTTGC+AGG	+	chr4_1:83842346-83842365	Msa0579220:exon	45.0%
	AGAGATCCTGAGGTATGATG+CGG	-	chr4_1:83842391-83842410	None:intergenic	45.0%
	TGTGAAAGACAGAGATCCTG+AGG	-	chr4_1:83842401-83842420	None:intergenic	45.0%
!	GTCCGCCGTATATCAACTTT+TGG	+	chr4_1:83842681-83842700	Msa0579220:intron	45.0%
	CAGATGAGAAGACAGAGACT+CGG	-	chr4_1:83843473-83843492	None:intergenic	45.0%
	GACGGAAAATCTGCAACCAT+TGG	+	chr4_1:83843730-83843749	Msa0579220:CDS	45.0%
	GGCATGATCAGTTATGCCAA+TGG	-	chr4_1:83843749-83843768	None:intergenic	45.0%
	TGAATTGCCAGAAGTGGGAA+CGG	+	chr4_1:83843798-83843817	Msa0579220:CDS	45.0%
!	TGGTGCAGTTGAAAGTGTGA+AGG	+	chr4_1:83843843-83843862	Msa0579220:CDS	45.0%
	GACATCAACTCTCCTGTTTC+AGG	+	chr4_1:83843874-83843893	Msa0579220:CDS	45.0%
	CAAGCCCCTATGAAAATGGA+TGG	+	chr4_1:83844070-83844089	Msa0579220:CDS	45.0%
	TCGGCAAGACTATCAATGAC+TGG	-	chr4_1:83844184-83844203	None:intergenic	45.0%
	GAGAGAGAGTGAAGAGTGGT+AGG	-	chr4_1:83842230-83842249	None:intergenic	50.0%
	CTGAGAGAGAGAGTGAAGAG+TGG	-	chr4_1:83842234-83842253	None:intergenic	50.0%
!	AGATGGCTTGCAGGCAGTTA+TGG	+	chr4_1:83842355-83842374	Msa0579220:exon	50.0%
!	GATGGCTTGCAGGCAGTTAT+GGG	+	chr4_1:83842356-83842375	Msa0579220:exon	50.0%
	ACGGCAGTGAAACAAGGAGA+TGG	+	chr4_1:83843817-83843836	Msa0579220:CDS	50.0%
	GAGAGAGAGTGAAGAGTGGT+AGG	-	chr4_1:83842230-83842249	None:intergenic	50.0%
	CTGAGAGAGAGAGTGAAGAG+TGG	-	chr4_1:83842234-83842253	None:intergenic	50.0%
!	AGATGGCTTGCAGGCAGTTA+TGG	+	chr4_1:83842355-83842374	Msa0579220:exon	50.0%
!	GATGGCTTGCAGGCAGTTAT+GGG	+	chr4_1:83842356-83842375	Msa0579220:exon	50.0%
	ACGGCAGTGAAACAAGGAGA+TGG	+	chr4_1:83843817-83843836	Msa0579220:CDS	50.0%
	TTCACTGCCGTTCCCACTTC+TGG	-	chr4_1:83843808-83843827	None:intergenic	55.0%
	GTGGGAACGGCAGTGAAACA+AGG	+	chr4_1:83843811-83843830	Msa0579220:CDS	55.0%
	GCTCAGCAACTCTCCTGCTT+TGG	+	chr4_1:83843921-83843940	Msa0579220:CDS	55.0%
!	TGCGCTAGCTTCTGATGCAG+AGG	+	chr4_1:83844305-83844324	Msa0579220:three_prime_UTR	55.0%
!	GCGCTAGCTTCTGATGCAGA+GGG	+	chr4_1:83844306-83844325	Msa0579220:three_prime_UTR	55.0%
	TTCACTGCCGTTCCCACTTC+TGG	-	chr4_1:83843808-83843827	None:intergenic	55.0%
	GTGGGAACGGCAGTGAAACA+AGG	+	chr4_1:83843811-83843830	Msa0579220:CDS	55.0%
	GCTCAGCAACTCTCCTGCTT+TGG	+	chr4_1:83843921-83843940	Msa0579220:CDS	55.0%
!	TGCGCTAGCTTCTGATGCAG+AGG	+	chr4_1:83844305-83844324	Msa0579220:three_prime_UTR	55.0%
!	GCGCTAGCTTCTGATGCAGA+GGG	+	chr4_1:83844306-83844325	Msa0579220:three_prime_UTR	55.0%
	CCTGAGGTATGATGCGGCTC+TGG	-	chr4_1:83842385-83842404	None:intergenic	60.0%
	CCAGAGCCGCATCATACCTC+AGG	+	chr4_1:83842382-83842401	Msa0579220:CDS	60.0%
	CCTGAGGTATGATGCGGCTC+TGG	-	chr4_1:83842385-83842404	None:intergenic	60.0%
	CCAGAGCCGCATCATACCTC+AGG	+	chr4_1:83842382-83842401	Msa0579220:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4_1	gene	83842213	83844645	83842213	ID=Msa0579220;Name=Msa0579220
chr4_1	mRNA	83842213	83844645	83842213	ID=Msa0579220-mRNA-1;Parent=Msa0579220;Name=Msa0579220-mRNA-1;_AED=0.06;_eAED=0.06;_QI=144\|1\|1\|1\|1\|1\|3\|454\|155
chr4_1	exon	83842213	83842435	83842213	ID=Msa0579220-mRNA-1:exon:30622;Parent=Msa0579220-mRNA-1
chr4_1	exon	83843683	83843942	83843683	ID=Msa0579220-mRNA-1:exon:30623;Parent=Msa0579220-mRNA-1
chr4_1	exon	83844063	83844645	83844063	ID=Msa0579220-mRNA-1:exon:30624;Parent=Msa0579220-mRNA-1
chr4_1	five_prime_UTR	83842213	83842356	83842213	ID=Msa0579220-mRNA-1:five_prime_utr;Parent=Msa0579220-mRNA-1
chr4_1	CDS	83842357	83842435	83842357	ID=Msa0579220-mRNA-1:cds;Parent=Msa0579220-mRNA-1
chr4_1	CDS	83843683	83843942	83843683	ID=Msa0579220-mRNA-1:cds;Parent=Msa0579220-mRNA-1
chr4_1	CDS	83844063	83844191	83844063	ID=Msa0579220-mRNA-1:cds;Parent=Msa0579220-mRNA-1
chr4_1	three_prime_UTR	83844192	83844645	83844192	ID=Msa0579220-mRNA-1:three_prime_utr;Parent=Msa0579220-mRNA-1

Gene Sequence

>Msa0579220

ATGGCTTGCAGGCAGTTATGGGCATCCAGAGCCGCATCATACCTCAGGATCTCTGTCTTTCACAGAACCTTCTCTAACGTTCTGAACAATCTGAAGTATGCTGATTCTCATGAATGGGTGAAAGTTGACGGAAAATCTGCAACCATTGGCATAACTGATCATGCCCAAGATCATTTGGGTGATGTTGTGTATGTTGAATTGCCAGAAGTGGGAACGGCAGTGAAACAAGGAGATGGTTTTGGTGCAGTTGAAAGTGTGAAGGCAACTAGTGACATCAACTCTCCTGTTTCAGGAAAAGTGATTGAAGTTAATGAAGAGCTCAGCAACTCTCCTGCTTTGGTCAACTCAAGCCCCTATGAAAATGGATGGATAATTAAAGTTGAGATGAGTGACAGTGGCGAACTTGACAAATTGATGGATTCAGAAAAGTATACCAAGTTCTGTGAAGAAGAAGATTCCAGTCATTGA

Protein sequence

>Msa0579220

MACRQLWASRAASYLRISVFHRTFSNVLNNLKYADSHEWVKVDGKSATIGITDHAQDHLGDVVYVELPEVGTAVKQGDGFGAVESVKATSDINSPVSGKVIEVNEELSNSPALVNSSPYENGWIIKVEMSDSGELDKLMDSEKYTKFCEEEDSSH*