AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0038290

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0038290 MtrunA17_Chr1g0201781 93.642 173 7 4 1 173 1 169 1.10e-90 261
Msa0038290 MtrunA17_Chr1g0211871 38.614 101 60 1 72 170 76 176 4.49e-24 92.8
Msa0038290 MtrunA17_Chr3g0125821 36.634 101 64 0 70 170 74 174 1.95e-22 88.6
Msa0038290 MtrunA17_Chr2g0317731 29.586 169 107 2 10 166 2 170 4.27e-22 87.4
Msa0038290 MtrunA17_Chr8g0360031 36.082 97 62 0 70 166 77 173 4.78e-22 87.4
Msa0038290 MtrunA17_Chr1g0155721 40.789 76 44 1 80 155 26 100 1.78e-19 79.3
Msa0038290 MtrunA17_Chr7g0216481 35.955 89 55 2 78 166 44 130 7.55e-19 78.2
Msa0038290 MtrunA17_Chr3g0142051 38.158 76 46 1 80 155 26 100 1.79e-18 76.6
Msa0038290 MtrunA17_Chr5g0404831 35.644 101 60 3 69 166 14 112 2.17e-17 73.9
Msa0038290 MtrunA17_Chr4g0044731 29.747 158 96 3 19 170 21 169 3.33e-17 74.7
Msa0038290 MtrunA17_Chr5g0415771 38.095 63 38 1 81 143 40 101 1.08e-15 69.7
Msa0038290 MtrunA17_Chr2g0280411 35.227 88 55 2 79 166 28 113 4.31e-14 65.5
Msa0038290 MtrunA17_Chr1g0194961 31.915 94 61 2 72 162 155 248 7.28e-14 68.2
Msa0038290 MtrunA17_Chr1g0194961 34.343 99 58 4 80 172 44 141 6.94e-11 59.7
Msa0038290 MtrunA17_Chr5g0438731 31.915 94 62 1 62 155 60 151 1.07e-13 67.8
Msa0038290 MtrunA17_Chr5g0438731 29.808 104 66 3 72 169 198 300 7.78e-12 62.8
Msa0038290 MtrunA17_Chr1g0200731 36.735 98 57 3 72 166 101 196 1.14e-13 67.8
Msa0038290 MtrunA17_Chr4g0042051 34.066 91 58 2 80 170 30 118 9.24e-13 62.0
Msa0038290 MtrunA17_Chr5g0415761 30.556 72 49 1 71 142 31 101 1.10e-12 61.6
Msa0038290 MtrunA17_Chr2g0287471 31.325 83 57 0 73 155 37 119 3.53e-12 63.5
Msa0038290 MtrunA17_Chr2g0317701 29.167 120 80 2 55 169 31 150 9.02e-12 60.1
Msa0038290 MtrunA17_Chr5g0415741 32.258 62 41 1 81 142 39 99 1.54e-11 58.5
Msa0038290 MtrunA17_Chr2g0317721 29.897 97 68 0 73 169 48 144 4.97e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0038290 AT1G76760.1 63.372 172 59 3 3 172 2 171 1.57e-67 203
Msa0038290 AT1G43560.1 64.286 154 50 2 25 173 14 167 8.01e-63 191
Msa0038290 AT1G50320.1 44.554 101 54 1 72 170 77 177 7.20e-26 97.8
Msa0038290 AT1G03680.1 35.714 98 63 0 72 169 82 179 2.14e-22 89.0
Msa0038290 AT3G15360.1 38.542 96 56 1 72 164 94 189 3.23e-20 83.6
Msa0038290 AT5G39950.1 35.227 88 55 2 78 165 43 128 1.80e-17 74.7
Msa0038290 AT4G03520.1 32.941 85 57 0 72 156 88 172 4.32e-17 75.1
Msa0038290 AT3G51030.1 41.026 78 44 2 79 155 24 100 7.42e-17 72.8
Msa0038290 AT1G59730.1 32.039 103 67 3 64 166 26 125 3.05e-16 71.6
Msa0038290 AT2G15570.1 28.302 106 76 0 65 170 67 172 5.03e-16 72.0
Msa0038290 AT2G15570.2 28.571 105 75 0 66 170 69 173 7.66e-16 71.6
Msa0038290 AT1G45145.1 35.294 85 53 2 81 165 26 108 2.33e-15 68.9
Msa0038290 AT1G19730.1 30.952 84 58 0 76 159 22 105 2.47e-14 66.2
Msa0038290 AT3G56420.1 36.250 80 50 1 80 159 60 138 8.53e-14 65.9
Msa0038290 AT3G56420.2 36.250 80 50 1 80 159 60 138 8.53e-14 65.9
Msa0038290 AT3G56420.3 36.250 80 50 1 80 159 60 138 8.53e-14 65.9
Msa0038290 AT1G04980.1 34.831 89 58 0 70 158 38 126 1.92e-13 67.8
Msa0038290 AT1G35620.1 37.500 96 55 3 70 162 38 131 3.46e-13 67.0
Msa0038290 AT5G42980.1 34.211 76 49 1 80 155 25 99 1.04e-12 62.0
Msa0038290 AT2G47470.1 29.032 93 63 2 73 162 150 242 1.10e-12 65.1
Msa0038290 AT2G47470.3 29.032 93 63 2 73 162 150 242 1.27e-12 65.1
Msa0038290 AT2G47470.4 29.167 96 65 2 73 165 150 245 1.31e-12 65.1
Msa0038290 AT1G69880.1 32.258 93 57 2 78 170 55 141 1.75e-12 62.0
Msa0038290 AT2G47470.2 29.167 96 65 2 73 165 150 245 1.87e-12 63.9
Msa0038290 AT2G32920.1 36.735 98 57 3 62 158 31 124 3.27e-12 63.9
Msa0038290 AT2G32920.1 30.337 89 59 2 72 158 170 257 8.97e-11 60.1
Msa0038290 AT3G17880.2 37.313 67 41 1 89 155 292 357 3.76e-12 63.9
Msa0038290 AT3G54960.2 32.258 93 62 1 70 162 105 196 3.83e-12 63.9
Msa0038290 AT3G17880.1 37.313 67 41 1 89 155 299 364 4.24e-12 63.5
Msa0038290 AT3G06730.1 30.588 170 108 6 3 169 21 183 4.37e-12 61.6
Msa0038290 AT3G08710.2 33.708 89 55 2 70 155 30 117 6.32e-12 60.5
Msa0038290 AT3G08710.3 33.708 89 55 2 70 155 30 117 6.32e-12 60.5
Msa0038290 AT3G08710.1 33.708 89 55 2 70 155 30 117 6.32e-12 60.5
Msa0038290 AT3G54960.1 32.258 93 62 1 70 162 105 196 2.85e-11 61.6
Msa0038290 AT5G16400.1 37.079 89 53 3 79 166 94 180 2.97e-11 59.7
Msa0038290 AT1G11530.1 29.885 87 59 2 80 166 22 106 6.71e-11 57.4

Find 64 sgRNAs with CRISPR-Local

Find 249 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCAACTTTCATCATCTTTA+AGG 0.037363 1_1:+67691511 Msa0038290:CDS
ATATCTATTATTTATTTCTT+TGG 0.190546 1_1:-67689604 None:intergenic
GTGTGGTCCTTGTCAGTTCA+TGG 0.318473 1_1:+67691379 Msa0038290:CDS
TATCTATTATTTATTTCTTT+GGG 0.318796 1_1:-67689603 None:intergenic
AACAATGTGCAAGTCATATT+AGG 0.342152 1_1:+67693130 Msa0038290:three_prime_UTR
CTGTGGTCAGGTTCAATTTG+TGG 0.343285 1_1:-67693091 None:intergenic
AACTGTACGAAAGTTGGATT+TGG 0.379518 1_1:+67693053 Msa0038290:three_prime_UTR
TGCCTCAATGTTGTATTTGT+TGG 0.387952 1_1:-67691486 None:intergenic
GAAACGAGGGCGGGAGGAAG+AGG 0.407611 1_1:-67689831 None:intergenic
CTCTTCTTCGTCCACACGTC+TGG 0.425949 1_1:+67689730 Msa0038290:CDS
AGAAAACCAACTGCTCTGTC+TGG 0.431411 1_1:-67689550 None:intergenic
CAAATCATCAAAAGAGGAAA+AGG 0.443022 1_1:-67691145 None:intergenic
ACTCCTCGCCTTCTTCCAAT+TGG 0.450067 1_1:+67689794 Msa0038290:CDS
TTTATTTCTTTGGGAATTGA+CGG 0.450323 1_1:-67689594 None:intergenic
AGTTGCAACTGTACGAAAGT+TGG 0.458586 1_1:+67693047 Msa0038290:three_prime_UTR
TGCTGAGATGGAAGCGGCCA+TGG 0.463484 1_1:-67689675 None:intergenic
GCATAGAAGTCAACAAATAC+AGG 0.470351 1_1:-67691186 None:intergenic
AGACATAAGCACCTGTGGTC+AGG 0.473819 1_1:-67693103 None:intergenic
AGTTTGTCAATCGGAATGTT+GGG 0.474535 1_1:+67692771 Msa0038290:three_prime_UTR
CAAAATGAGAACGAACAGAT+TGG 0.476905 1_1:-67689648 None:intergenic
AGGAAGTAGGAAACGAGGGC+GGG 0.481188 1_1:-67689840 None:intergenic
GAGTTTGTCAATCGGAATGT+TGG 0.483482 1_1:+67692770 Msa0038290:three_prime_UTR
GAAAGACGGCGAGTACCAAT+TGG 0.490211 1_1:-67689809 None:intergenic
CAACTTACAAAGCGATCAAA+GGG 0.495557 1_1:-67691542 None:intergenic
CACATACATGGTTGGATAGG+AGG 0.496043 1_1:+67692796 Msa0038290:three_prime_UTR
GTCAACAAATACAGGCTTGT+CGG 0.497227 1_1:-67691178 None:intergenic
TCCTTAAAGATGATGAAAGT+TGG 0.497295 1_1:-67691512 None:intergenic
ACTTTCATCATCTTTAAGGA+TGG 0.499489 1_1:+67691515 Msa0038290:CDS
AGTCGAAGACGCTGCTGAGA+TGG 0.500444 1_1:-67689687 None:intergenic
TGCAAGCAAATCATCAAAAG+AGG 0.504002 1_1:-67691151 None:intergenic
GACCACAGGTGCTTATGTCT+TGG 0.514527 1_1:+67693106 Msa0038290:three_prime_UTR
CCAACTTACAAAGCGATCAA+AGG 0.517391 1_1:-67691543 None:intergenic
GGCGAGGAGTTCGAAATTGA+AGG 0.521846 1_1:-67689781 None:intergenic
AAGTAGGAAACGAGGGCGGG+AGG 0.525596 1_1:-67689837 None:intergenic
GGCGGGAGGAAGAGGAAAGA+CGG 0.525873 1_1:-67689823 None:intergenic
AAAAGTCCAGACAGAGCAGT+TGG 0.528989 1_1:+67689544 Msa0038290:five_prime_UTR
GTTCATGGTTCCTGTACTTG+AGG 0.555079 1_1:+67691394 Msa0038290:CDS
GCGCTCACTTCCTCAAGTAC+AGG 0.556326 1_1:-67691404 None:intergenic
GTTGGGATCACATACATGGT+TGG 0.565508 1_1:+67692788 Msa0038290:three_prime_UTR
AGGAGTGAAAGGGAGAGGAA+AGG 0.570746 1_1:+67689515 None:intergenic
ACGAAGAAGAGTTCAAAGAA+TGG 0.574201 1_1:-67689718 None:intergenic
GATCACATACATGGTTGGAT+AGG 0.582401 1_1:+67692793 Msa0038290:three_prime_UTR
TTTCAGTTAAATGAAGACAC+AGG 0.583049 1_1:+67692748 Msa0038290:intron
CACAAATTGAACCTGACCAC+AGG 0.583927 1_1:+67693092 Msa0038290:three_prime_UTR
GAGGAAGTAGGAAACGAGGG+CGG 0.585576 1_1:-67689841 None:intergenic
GGCGAGTACCAATTGGAAGA+AGG 0.589024 1_1:-67689802 None:intergenic
AGACGCTGCTGAGATGGAAG+CGG 0.589399 1_1:-67689681 None:intergenic
TTGTATTTGTTGGCAATGCT+AGG 0.592667 1_1:-67691476 None:intergenic
AGACACAGGAGTTTGTCAAT+CGG 0.598830 1_1:+67692762 Msa0038290:three_prime_UTR
TTGTTGACTTCTATGCAACC+TGG 0.598842 1_1:+67691193 Msa0038290:CDS
GAATGTTGGGATCACATACA+TGG 0.601877 1_1:+67692784 Msa0038290:three_prime_UTR
TTATTGCTTAACGTTGAGAG+TGG 0.611598 1_1:-67692594 None:intergenic
TACTTGAGGAAGTGAGCGCT+CGG 0.618555 1_1:+67691408 Msa0038290:CDS
TCAACGTTAAGCAATAACTC+TGG 0.625204 1_1:+67692600 Msa0038290:exon
TGTTACTTACGAGAGGAAGT+AGG 0.626215 1_1:-67689853 None:intergenic
AAGAAGAGTTCAAAGAATGG+AGG 0.637507 1_1:-67689715 None:intergenic
GTACCAATTGGAAGAAGGCG+AGG 0.648368 1_1:-67689797 None:intergenic
TGCCAAGACATAAGCACCTG+TGG 0.649363 1_1:-67693108 None:intergenic
CGAGAGGAAGTAGGAAACGA+GGG 0.659041 1_1:-67689844 None:intergenic
TGCCAACAAATACAACATTG+AGG 0.660640 1_1:+67691484 Msa0038290:CDS
ATTGGATAATCAGAAAACAA+TGG 0.661635 1_1:-67689630 None:intergenic
ACAGGAACCATGAACTGACA+AGG 0.685218 1_1:-67691386 None:intergenic
ACGAGAGGAAGTAGGAAACG+AGG 0.685474 1_1:-67689845 None:intergenic
TGATGAAGGTACCAGACGTG+TGG 0.715833 1_1:-67689741 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATCTATTATTTATTTCTTT+GGG - chr1_1:67689606-67689625 None:intergenic 10.0%
!!! ATATCTATTATTTATTTCTT+TGG - chr1_1:67689607-67689626 None:intergenic 10.0%
!! AATTCAATAAGTATATATAT+GGG - chr1_1:67690404-67690423 None:intergenic 10.0%
!! AAATTCAATAAGTATATATA+TGG - chr1_1:67690405-67690424 None:intergenic 10.0%
!!! TAAACAATCAAATTTTTTAT+TGG - chr1_1:67692892-67692911 None:intergenic 10.0%
!!! TATATAATTTAGTTTTGATT+TGG + chr1_1:67693010-67693029 Msa0038290:three_prime_UTR 10.0%
!!! ATATAATTTAGTTTTGATTT+GGG + chr1_1:67693011-67693030 Msa0038290:three_prime_UTR 10.0%
!! TAATAATGGTTATCATAAAA+CGG - chr1_1:67689915-67689934 None:intergenic 15.0%
!! TGAAGAAAGAATAATAATAA+TGG - chr1_1:67689929-67689948 None:intergenic 15.0%
!!! ATTTTATTTATACTTGTTGT+TGG + chr1_1:67689951-67689970 Msa0038290:intron 15.0%
!! TAATAAATTAGTGAATAACT+TGG - chr1_1:67689982-67690001 None:intergenic 15.0%
!!! TAATTTTTTTGGAATACAAA+AGG + chr1_1:67690696-67690715 Msa0038290:intron 15.0%
!! CAAGTATCATTTAAATAAAA+TGG - chr1_1:67692658-67692677 None:intergenic 15.0%
!!! TATAATTTAGTTTTGATTTG+GGG + chr1_1:67693012-67693031 Msa0038290:three_prime_UTR 15.0%
!! AAGGAGAAAAATAAAATAGT+TGG - chr1_1:67689886-67689905 None:intergenic 20.0%
!! AATAAGTATATATATGGGTT+TGG - chr1_1:67690399-67690418 None:intergenic 20.0%
!!! ATCCAATTTTTTCCTATATT+TGG + chr1_1:67690641-67690660 Msa0038290:intron 20.0%
!! ATCCAAATATAGGAAAAAAT+TGG - chr1_1:67690646-67690665 None:intergenic 20.0%
!! AAAAAAATTAGCGTAAATTC+TGG - chr1_1:67690686-67690705 None:intergenic 20.0%
!!! AGAATTTACGCTAATTTTTT+TGG + chr1_1:67690685-67690704 Msa0038290:intron 20.0%
!! TTAATGATTTAAATCGAATG+CGG - chr1_1:67691618-67691637 None:intergenic 20.0%
!! TCAGTAAGTTAAAAGAATAT+CGG - chr1_1:67691707-67691726 None:intergenic 20.0%
!!! TTATAGATAGTTAGTTTCAA+TGG + chr1_1:67691766-67691785 Msa0038290:intron 20.0%
!!! AAAATACACAGAAATCTTTT+CGG + chr1_1:67691871-67691890 Msa0038290:intron 20.0%
!! ACAATTTACAACAATAAGAA+AGG - chr1_1:67692254-67692273 None:intergenic 20.0%
!!! CATGCATATAATTAATTTGT+AGG + chr1_1:67692331-67692350 Msa0038290:intron 20.0%
!! AATATACAAAACATCAATGT+AGG - chr1_1:67692440-67692459 None:intergenic 20.0%
!!! ACCAAAATTTTATCTAACTT+GGG - chr1_1:67692516-67692535 None:intergenic 20.0%
!!! ATGTACAAGAATTTTGATAT+TGG - chr1_1:67692988-67693007 None:intergenic 20.0%
!!! ATAATTTAGTTTTGATTTGG+GGG + chr1_1:67693013-67693032 Msa0038290:three_prime_UTR 20.0%
!! TTTATTTCTTTGGGAATTGA+CGG - chr1_1:67689597-67689616 None:intergenic 25.0%
! ATTGGATAATCAGAAAACAA+TGG - chr1_1:67689633-67689652 None:intergenic 25.0%
!! TGAAATATATAGTGTTGGAA+GGG - chr1_1:67690163-67690182 None:intergenic 25.0%
! TTCCAACACTATATATTTCA+CGG + chr1_1:67690163-67690182 Msa0038290:intron 25.0%
! AATCAAAAGGTCTAAGTTTA+GGG - chr1_1:67690243-67690262 None:intergenic 25.0%
! AATTTAAGCACTTATAAGCA+GGG + chr1_1:67690420-67690439 Msa0038290:intron 25.0%
! AACTACAAATTTACTAAACG+CGG + chr1_1:67690722-67690741 Msa0038290:intron 25.0%
! ACTGTTAAACTGTTAAACTA+TGG + chr1_1:67690982-67691001 Msa0038290:intron 25.0%
! AAAACAATATCCAAAAATGC+TGG - chr1_1:67691100-67691119 None:intergenic 25.0%
! AGTAAGTTAAAAGAATATCG+GGG - chr1_1:67691705-67691724 None:intergenic 25.0%
! CAGTAAGTTAAAAGAATATC+GGG - chr1_1:67691706-67691725 None:intergenic 25.0%
! AATCAAATGCAGTATCATTA+TGG - chr1_1:67691836-67691855 None:intergenic 25.0%
! CTTAACCAAAATTAGGAATT+AGG - chr1_1:67691974-67691993 None:intergenic 25.0%
! ATCATGTCTTAACCAAAATT+AGG - chr1_1:67691981-67692000 None:intergenic 25.0%
!!! TTTTGGTTAAGACATGATAT+TGG + chr1_1:67691983-67692002 Msa0038290:intron 25.0%
! ACTTCAACATAATGAAATTG+AGG + chr1_1:67692042-67692061 Msa0038290:intron 25.0%
! TTAGAATGTAAAGAACCTAA+AGG + chr1_1:67692074-67692093 Msa0038290:intron 25.0%
! TCTACTTAAAAACAACCTTT+AGG - chr1_1:67692092-67692111 None:intergenic 25.0%
!! CCAAAATTTTATCTAACTTG+GGG - chr1_1:67692515-67692534 None:intergenic 25.0%
!! GACCAAAATTTTATCTAACT+TGG - chr1_1:67692517-67692536 None:intergenic 25.0%
! ATTTAAATGATACTTGCTTC+AGG + chr1_1:67692662-67692681 Msa0038290:intron 25.0%
! TTTAACTGAAAAAAGTAGCA+AGG - chr1_1:67692739-67692758 None:intergenic 25.0%
!!! CAATCAAATTTTTTATTGGC+TGG - chr1_1:67692888-67692907 None:intergenic 25.0%
TATCATAAAACGGAAAGGAA+AGG - chr1_1:67689905-67689924 None:intergenic 30.0%
ATGGTTATCATAAAACGGAA+AGG - chr1_1:67689910-67689929 None:intergenic 30.0%
GGTAATATGATTCAGAAATG+AGG - chr1_1:67690142-67690161 None:intergenic 30.0%
! GTGAAATATATAGTGTTGGA+AGG - chr1_1:67690164-67690183 None:intergenic 30.0%
CAATCAAAAGGTCTAAGTTT+AGG - chr1_1:67690244-67690263 None:intergenic 30.0%
GAAAAACCATATTCCTTTCA+CGG - chr1_1:67690365-67690384 None:intergenic 30.0%
GAATTTAAGCACTTATAAGC+AGG + chr1_1:67690419-67690438 Msa0038290:intron 30.0%
ATTATGTCGTGTCAAAATTG+CGG + chr1_1:67690541-67690560 Msa0038290:intron 30.0%
!! TGTAAGCAGGGTTTTAATAA+AGG - chr1_1:67690574-67690593 None:intergenic 30.0%
! ACTGTTAAACTATGGCTTTA+AGG + chr1_1:67690990-67691009 Msa0038290:intron 30.0%
! TTTTGGATATTGTTTTCTGC+AGG + chr1_1:67691104-67691123 Msa0038290:intron 30.0%
CAAATCATCAAAAGAGGAAA+AGG - chr1_1:67691148-67691167 None:intergenic 30.0%
CATGAAATATAACCAGCTAA+AGG - chr1_1:67691285-67691304 None:intergenic 30.0%
TCCTTAAAGATGATGAAAGT+TGG - chr1_1:67691515-67691534 None:intergenic 30.0%
! ACTTTCATCATCTTTAAGGA+TGG + chr1_1:67691515-67691534 Msa0038290:CDS 30.0%
CATGAAAAATTGTGATGTCA+AGG + chr1_1:67691803-67691822 Msa0038290:intron 30.0%
! TTCTGTGTATTTTCTATGAC+TGG - chr1_1:67691864-67691883 None:intergenic 30.0%
! AATCGCCTAATTCCTAATTT+TGG + chr1_1:67691966-67691985 Msa0038290:intron 30.0%
! TAAGTAGAAACTTTTGTCTG+TGG + chr1_1:67692104-67692123 Msa0038290:intron 30.0%
CGTCATATTTGAAAAAGTGA+AGG + chr1_1:67692174-67692193 Msa0038290:intron 30.0%
TTGAAAAAGTGAAGGAATGA+AGG + chr1_1:67692182-67692201 Msa0038290:intron 30.0%
TAAATCTCATCACAGTCATT+TGG + chr1_1:67692283-67692302 Msa0038290:intron 30.0%
TGACAAGACAAAAGAAAACT+AGG + chr1_1:67692460-67692479 Msa0038290:intron 30.0%
ACAAAAGAAAACTAGGATGT+TGG + chr1_1:67692467-67692486 Msa0038290:intron 30.0%
! AATTTTATCTAACTTGGGGA+AGG - chr1_1:67692511-67692530 None:intergenic 30.0%
! CCCCAAGTTAGATAAAATTT+TGG + chr1_1:67692512-67692531 Msa0038290:intron 30.0%
!! TTTTGGTCTTCATGATTTTC+AGG + chr1_1:67692529-67692548 Msa0038290:intron 30.0%
! ATAAAATGGTACTGAATCGA+TGG - chr1_1:67692644-67692663 None:intergenic 30.0%
! AAAGTAGCAAGGTATTTAGA+GGG - chr1_1:67692728-67692747 None:intergenic 30.0%
! AAAAGTAGCAAGGTATTTAG+AGG - chr1_1:67692729-67692748 None:intergenic 30.0%
TTTCAGTTAAATGAAGACAC+AGG + chr1_1:67692748-67692767 Msa0038290:intron 30.0%
TGGATCTTGGCAAAAATAAT+GGG - chr1_1:67692868-67692887 None:intergenic 30.0%
!!! ATTTTTTATTGGCTGGATCT+TGG - chr1_1:67692881-67692900 None:intergenic 30.0%
CAAAATGAGAACGAACAGAT+TGG - chr1_1:67689651-67689670 None:intergenic 35.0%
AAGAAGAGTTCAAAGAATGG+AGG - chr1_1:67689718-67689737 None:intergenic 35.0%
ACGAAGAAGAGTTCAAAGAA+TGG - chr1_1:67689721-67689740 None:intergenic 35.0%
! GAAGATGATGATGATGATGA+AGG - chr1_1:67689758-67689777 None:intergenic 35.0%
!! TTAGTAGTGTTACTTACGAG+AGG - chr1_1:67689863-67689882 None:intergenic 35.0%
TAGTGCACTTTCTAGATGTT+TGG + chr1_1:67690047-67690066 Msa0038290:intron 35.0%
AGTGCACTTTCTAGATGTTT+GGG + chr1_1:67690048-67690067 Msa0038290:intron 35.0%
ATGATGAGTTCAAAATGAGC+AGG - chr1_1:67690098-67690117 None:intergenic 35.0%
GTCCGTGAAATATATAGTGT+TGG - chr1_1:67690168-67690187 None:intergenic 35.0%
CACTATATATTTCACGGACT+AGG + chr1_1:67690169-67690188 Msa0038290:intron 35.0%
!!! TAGGCTTTTGTTTTGGTGTT+TGG + chr1_1:67690188-67690207 Msa0038290:intron 35.0%
TTGTGAGCATGTATGTAATC+AGG - chr1_1:67690216-67690235 None:intergenic 35.0%
CCATCCACCAAAAATCTTTA+GGG + chr1_1:67690264-67690283 Msa0038290:intron 35.0%
! AACCAGACCCTAAAGATTTT+TGG - chr1_1:67690274-67690293 None:intergenic 35.0%
!! AAAAATCTTTAGGGTCTGGT+TGG + chr1_1:67690273-67690292 Msa0038290:intron 35.0%
CTGCAATTGCAATCATGTTA+TGG + chr1_1:67690497-67690516 Msa0038290:intron 35.0%
GAATTTATGACGTGTAAGCA+GGG - chr1_1:67690586-67690605 None:intergenic 35.0%
AGAATTTATGACGTGTAAGC+AGG - chr1_1:67690587-67690606 None:intergenic 35.0%
! AATTATCGCCTGATTTGATC+AGG + chr1_1:67690773-67690792 Msa0038290:intron 35.0%
! ATTATCGCCTGATTTGATCA+GGG + chr1_1:67690774-67690793 Msa0038290:intron 35.0%
ATCAGGGCAATCATCATAAA+TGG + chr1_1:67690790-67690809 Msa0038290:intron 35.0%
TTGCAAGCATGAATGAACAT+GGG - chr1_1:67691023-67691042 None:intergenic 35.0%
ATTGCAAGCATGAATGAACA+TGG - chr1_1:67691024-67691043 None:intergenic 35.0%
TGCAAGCAAATCATCAAAAG+AGG - chr1_1:67691154-67691173 None:intergenic 35.0%
GCATAGAAGTCAACAAATAC+AGG - chr1_1:67691189-67691208 None:intergenic 35.0%
!!! GAAGCATTTTTTCCTTTAGC+TGG + chr1_1:67691270-67691289 Msa0038290:intron 35.0%
! TTGTATTTGTTGGCAATGCT+AGG - chr1_1:67691479-67691498 None:intergenic 35.0%
TGCCAACAAATACAACATTG+AGG + chr1_1:67691484-67691503 Msa0038290:CDS 35.0%
TGCCTCAATGTTGTATTTGT+TGG - chr1_1:67691489-67691508 None:intergenic 35.0%
GCCAACTTTCATCATCTTTA+AGG + chr1_1:67691511-67691530 Msa0038290:CDS 35.0%
CAACTTACAAAGCGATCAAA+GGG - chr1_1:67691545-67691564 None:intergenic 35.0%
TGTAGAAGACTTGTATGTAG+AGG + chr1_1:67691943-67691962 Msa0038290:intron 35.0%
TGTCTGTGGTCTATAACATA+TGG + chr1_1:67692118-67692137 Msa0038290:intron 35.0%
TTATTGCTTAACGTTGAGAG+TGG - chr1_1:67692597-67692616 None:intergenic 35.0%
TCAACGTTAAGCAATAACTC+TGG + chr1_1:67692600-67692619 Msa0038290:exon 35.0%
AGTTTGTCAATCGGAATGTT+GGG + chr1_1:67692771-67692790 Msa0038290:three_prime_UTR 35.0%
CTGGATCTTGGCAAAAATAA+TGG - chr1_1:67692869-67692888 None:intergenic 35.0%
AACTGTACGAAAGTTGGATT+TGG + chr1_1:67693053-67693072 Msa0038290:three_prime_UTR 35.0%
!!! ACAGAGCAGTTGGTTTTCTT+GGG + chr1_1:67689554-67689573 Msa0038290:five_prime_UTR 40.0%
!!! CGTTCTCATTTTGACTACCA+TGG + chr1_1:67689658-67689677 Msa0038290:exon 40.0%
! TGTTACTTACGAGAGGAAGT+AGG - chr1_1:67689856-67689875 None:intergenic 40.0%
GTGCACTTTCTAGATGTTTG+GGG + chr1_1:67690049-67690068 Msa0038290:intron 40.0%
! TTTGGTGGATGGCAATCAAA+AGG - chr1_1:67690256-67690275 None:intergenic 40.0%
GCCATCCACCAAAAATCTTT+AGG + chr1_1:67690263-67690282 Msa0038290:intron 40.0%
!!! CCCTAAAGATTTTTGGTGGA+TGG - chr1_1:67690267-67690286 None:intergenic 40.0%
!! CAGACCCTAAAGATTTTTGG+TGG - chr1_1:67690271-67690290 None:intergenic 40.0%
!! CACCAAAAATCTTTAGGGTC+TGG + chr1_1:67690269-67690288 Msa0038290:intron 40.0%
! GTTGGTTCAAGTTCAACAGA+GGG + chr1_1:67690295-67690314 Msa0038290:intron 40.0%
! TTGGTTCAAGTTCAACAGAG+GGG + chr1_1:67690296-67690315 Msa0038290:intron 40.0%
CCATATTCCTTTCACGGTTT+GGG - chr1_1:67690359-67690378 None:intergenic 40.0%
ACCATATTCCTTTCACGGTT+TGG - chr1_1:67690360-67690379 None:intergenic 40.0%
TGGGGCAACAATCCAAATAT+AGG - chr1_1:67690656-67690675 None:intergenic 40.0%
GTAAATTCTGGCGATCAATG+GGG - chr1_1:67690674-67690693 None:intergenic 40.0%
CGTAAATTCTGGCGATCAAT+GGG - chr1_1:67690675-67690694 None:intergenic 40.0%
ATGATTGCCCTGATCAAATC+AGG - chr1_1:67690784-67690803 None:intergenic 40.0%
GAGTCATGTTTATCGTGAAG+CGG - chr1_1:67690870-67690889 None:intergenic 40.0%
AACGATTTAGCAGCTAGACT+GGG - chr1_1:67690905-67690924 None:intergenic 40.0%
AAACGATTTAGCAGCTAGAC+TGG - chr1_1:67690906-67690925 None:intergenic 40.0%
AACATCCCATAACAGTACCT+CGG + chr1_1:67690935-67690954 Msa0038290:intron 40.0%
ATCTCGAGTATCAGAAGTAG+AGG - chr1_1:67690961-67690980 None:intergenic 40.0%
! TGAGAACTGACCAGCATTTT+TGG + chr1_1:67691087-67691106 Msa0038290:intron 40.0%
GTCAACAAATACAGGCTTGT+CGG - chr1_1:67691181-67691200 None:intergenic 40.0%
!! TTGTTGACTTCTATGCAACC+TGG + chr1_1:67691193-67691212 Msa0038290:CDS 40.0%
AAGAAAATGGAAAACGCACG+GGG - chr1_1:67691228-67691247 None:intergenic 40.0%
GAAGAAAATGGAAAACGCAC+GGG - chr1_1:67691229-67691248 None:intergenic 40.0%
GGAAGAAAATGGAAAACGCA+CGG - chr1_1:67691230-67691249 None:intergenic 40.0%
ATGAAGAGAGGGGAAGAAAA+TGG - chr1_1:67691241-67691260 None:intergenic 40.0%
TCAAACCCAACATGAAGAGA+GGG - chr1_1:67691252-67691271 None:intergenic 40.0%
TTCAAACCCAACATGAAGAG+AGG - chr1_1:67691253-67691272 None:intergenic 40.0%
TCTAATCAGTAACCCAATGC+TGG + chr1_1:67691349-67691368 Msa0038290:intron 40.0%
!! GTAACCCAATGCTGGTTTAT+AGG + chr1_1:67691357-67691376 Msa0038290:intron 40.0%
CACACCTATAAACCAGCATT+GGG - chr1_1:67691364-67691383 None:intergenic 40.0%
CCAACTTACAAAGCGATCAA+AGG - chr1_1:67691546-67691565 None:intergenic 40.0%
CCTTTGATCGCTTTGTAAGT+TGG + chr1_1:67691543-67691562 Msa0038290:intron 40.0%
! AAATCTTTTCGGCTTCTTGC+TGG + chr1_1:67691882-67691901 Msa0038290:intron 40.0%
! AATCTTTTCGGCTTCTTGCT+GGG + chr1_1:67691883-67691902 Msa0038290:intron 40.0%
CACCTTTACAGACACTACTA+AGG + chr1_1:67692012-67692031 Msa0038290:intron 40.0%
AGCCTTAGTAGTGTCTGTAA+AGG - chr1_1:67692017-67692036 None:intergenic 40.0%
GAAGATAGCTTGACATAGTC+TGG + chr1_1:67692204-67692223 Msa0038290:intron 40.0%
!! TGGTCTTCATGATTTTCAGG+AGG + chr1_1:67692532-67692551 Msa0038290:intron 40.0%
!! GGTCTTCATGATTTTCAGGA+GGG + chr1_1:67692533-67692552 Msa0038290:intron 40.0%
! ATTTAGAGGGCAGGGATTTT+TGG - chr1_1:67692715-67692734 None:intergenic 40.0%
AGACACAGGAGTTTGTCAAT+CGG + chr1_1:67692762-67692781 Msa0038290:three_prime_UTR 40.0%
GAGTTTGTCAATCGGAATGT+TGG + chr1_1:67692770-67692789 Msa0038290:three_prime_UTR 40.0%
GAATGTTGGGATCACATACA+TGG + chr1_1:67692784-67692803 Msa0038290:three_prime_UTR 40.0%
GATCACATACATGGTTGGAT+AGG + chr1_1:67692793-67692812 Msa0038290:three_prime_UTR 40.0%
AGTTGCAACTGTACGAAAGT+TGG + chr1_1:67693047-67693066 Msa0038290:three_prime_UTR 40.0%
AAAAGTCCAGACAGAGCAGT+TGG + chr1_1:67689544-67689563 Msa0038290:five_prime_UTR 45.0%
AGAAAACCAACTGCTCTGTC+TGG - chr1_1:67689553-67689572 None:intergenic 45.0%
!!! GACAGAGCAGTTGGTTTTCT+TGG + chr1_1:67689553-67689572 Msa0038290:five_prime_UTR 45.0%
!!! CACGGACTAGGCTTTTGTTT+TGG + chr1_1:67690181-67690200 Msa0038290:intron 45.0%
!! ATCTTTAGGGTCTGGTTGGT+TGG + chr1_1:67690277-67690296 Msa0038290:intron 45.0%
! GGTTGGTTCAAGTTCAACAG+AGG + chr1_1:67690294-67690313 Msa0038290:intron 45.0%
CCCAAACCGTGAAAGGAATA+TGG + chr1_1:67690356-67690375 Msa0038290:intron 45.0%
AATCACGATCCTTGATGCTG+TGG + chr1_1:67690454-67690473 Msa0038290:intron 45.0%
CATGATTCTCCACAGCATCA+AGG - chr1_1:67690466-67690485 None:intergenic 45.0%
CTTGATGCTGTGGAGAATCA+TGG + chr1_1:67690464-67690483 Msa0038290:intron 45.0%
TGGAGAATCATGGTCAAACG+CGG + chr1_1:67690474-67690493 Msa0038290:intron 45.0%
GCGTAAATTCTGGCGATCAA+TGG - chr1_1:67690676-67690695 None:intergenic 45.0%
TAATTCGATGTTGACGCTGC+TGG - chr1_1:67690758-67690777 None:intergenic 45.0%
!! ATCGTGAAGCGGATTTTGTG+CGG - chr1_1:67690859-67690878 None:intergenic 45.0%
!! GAGGTACTGTTATGGGATGT+TGG - chr1_1:67690936-67690955 None:intergenic 45.0%
TTCTTCCCCTCTCTTCATGT+TGG + chr1_1:67691243-67691262 Msa0038290:intron 45.0%
TCTTCCCCTCTCTTCATGTT+GGG + chr1_1:67691244-67691263 Msa0038290:intron 45.0%
CAAACCCAACATGAAGAGAG+GGG - chr1_1:67691251-67691270 None:intergenic 45.0%
CCACACCTATAAACCAGCAT+TGG - chr1_1:67691365-67691384 None:intergenic 45.0%
!! CCAATGCTGGTTTATAGGTG+TGG + chr1_1:67691362-67691381 Msa0038290:intron 45.0%
ACAGGAACCATGAACTGACA+AGG - chr1_1:67691389-67691408 None:intergenic 45.0%
GTTCATGGTTCCTGTACTTG+AGG + chr1_1:67691394-67691413 Msa0038290:CDS 45.0%
ATAGTCTGGACTCTGGTGTT+TGG + chr1_1:67692218-67692237 Msa0038290:intron 45.0%
CAATGTAGGCTCACAATGCT+TGG - chr1_1:67692426-67692445 None:intergenic 45.0%
! AGCAAGGTATTTAGAGGGCA+GGG - chr1_1:67692723-67692742 None:intergenic 45.0%
! TAGCAAGGTATTTAGAGGGC+AGG - chr1_1:67692724-67692743 None:intergenic 45.0%
GTTGGGATCACATACATGGT+TGG + chr1_1:67692788-67692807 Msa0038290:three_prime_UTR 45.0%
CACATACATGGTTGGATAGG+AGG + chr1_1:67692796-67692815 Msa0038290:three_prime_UTR 45.0%
CTGTGGTCAGGTTCAATTTG+TGG - chr1_1:67693094-67693113 None:intergenic 45.0%
CACAAATTGAACCTGACCAC+AGG + chr1_1:67693092-67693111 Msa0038290:three_prime_UTR 45.0%
!! TGATGAAGGTACCAGACGTG+TGG - chr1_1:67689744-67689763 None:intergenic 50.0%
GGCGAGGAGTTCGAAATTGA+AGG - chr1_1:67689784-67689803 None:intergenic 50.0%
ACTCCTCGCCTTCTTCCAAT+TGG + chr1_1:67689794-67689813 Msa0038290:CDS 50.0%
GTACCAATTGGAAGAAGGCG+AGG - chr1_1:67689800-67689819 None:intergenic 50.0%
GGCGAGTACCAATTGGAAGA+AGG - chr1_1:67689805-67689824 None:intergenic 50.0%
GAAAGACGGCGAGTACCAAT+TGG - chr1_1:67689812-67689831 None:intergenic 50.0%
CGAGAGGAAGTAGGAAACGA+GGG - chr1_1:67689847-67689866 None:intergenic 50.0%
ACGAGAGGAAGTAGGAAACG+AGG - chr1_1:67689848-67689867 None:intergenic 50.0%
TCAACAGAGGGGAGCGAAAA+TGG + chr1_1:67690307-67690326 Msa0038290:intron 50.0%
CAACAGAGGGGAGCGAAAAT+GGG + chr1_1:67690308-67690327 Msa0038290:intron 50.0%
! AGGGGAGCGAAAATGGGTTT+AGG + chr1_1:67690314-67690333 Msa0038290:intron 50.0%
! GGGGAGCGAAAATGGGTTTA+GGG + chr1_1:67690315-67690334 Msa0038290:intron 50.0%
! GGGAGCGAAAATGGGTTTAG+GGG + chr1_1:67690316-67690335 Msa0038290:intron 50.0%
! GGAGCGAAAATGGGTTTAGG+GGG + chr1_1:67690317-67690336 Msa0038290:intron 50.0%
! TTTCACGGTTTGGGTAGAGG+GGG - chr1_1:67690350-67690369 None:intergenic 50.0%
! CTTTCACGGTTTGGGTAGAG+GGG - chr1_1:67690351-67690370 None:intergenic 50.0%
! CCTTTCACGGTTTGGGTAGA+GGG - chr1_1:67690352-67690371 None:intergenic 50.0%
! TCCTTTCACGGTTTGGGTAG+AGG - chr1_1:67690353-67690372 None:intergenic 50.0%
TTCTGCACCAATCCCCATCA+TGG + chr1_1:67690823-67690842 Msa0038290:intron 50.0%
! GTCTTTGCCATGATGGGGAT+TGG - chr1_1:67690833-67690852 None:intergenic 50.0%
GGATGGTCTTTGCCATGATG+GGG - chr1_1:67690838-67690857 None:intergenic 50.0%
CGGATGGTCTTTGCCATGAT+GGG - chr1_1:67690839-67690858 None:intergenic 50.0%
!! TGAAGCGGATTTTGTGCGGA+TGG - chr1_1:67690855-67690874 None:intergenic 50.0%
!! AGAGGCCGAGGTACTGTTAT+GGG - chr1_1:67690943-67690962 None:intergenic 50.0%
!! TAGAGGCCGAGGTACTGTTA+TGG - chr1_1:67690944-67690963 None:intergenic 50.0%
AGTATCAGAAGTAGAGGCCG+AGG - chr1_1:67690955-67690974 None:intergenic 50.0%
GTGTGGTCCTTGTCAGTTCA+TGG + chr1_1:67691379-67691398 Msa0038290:CDS 50.0%
TACTTGAGGAAGTGAGCGCT+CGG + chr1_1:67691408-67691427 Msa0038290:CDS 50.0%
GCTTGACATAGTCTGGACTC+TGG + chr1_1:67692211-67692230 Msa0038290:intron 50.0%
!! GGCAGGGATTTTTGGAGAGA+TGG - chr1_1:67692707-67692726 None:intergenic 50.0%
AGACATAAGCACCTGTGGTC+AGG - chr1_1:67693106-67693125 None:intergenic 50.0%
GACCACAGGTGCTTATGTCT+TGG + chr1_1:67693106-67693125 Msa0038290:three_prime_UTR 50.0%
TGCCAAGACATAAGCACCTG+TGG - chr1_1:67693111-67693130 None:intergenic 50.0%
AGACGCTGCTGAGATGGAAG+CGG - chr1_1:67689684-67689703 None:intergenic 55.0%
AGTCGAAGACGCTGCTGAGA+TGG - chr1_1:67689690-67689709 None:intergenic 55.0%
CTCTTCTTCGTCCACACGTC+TGG + chr1_1:67689730-67689749 Msa0038290:CDS 55.0%
AGGAAGTAGGAAACGAGGGC+GGG - chr1_1:67689843-67689862 None:intergenic 55.0%
GAGGAAGTAGGAAACGAGGG+CGG - chr1_1:67689844-67689863 None:intergenic 55.0%
CCCTCTACCCAAACCGTGAA+AGG + chr1_1:67690349-67690368 Msa0038290:intron 55.0%
GCGGATGGTCTTTGCCATGA+TGG - chr1_1:67690840-67690859 None:intergenic 55.0%
GCGCTCACTTCCTCAAGTAC+AGG - chr1_1:67691407-67691426 None:intergenic 55.0%
TGCTGAGATGGAAGCGGCCA+TGG - chr1_1:67689678-67689697 None:intergenic 60.0%
GGCGGGAGGAAGAGGAAAGA+CGG - chr1_1:67689826-67689845 None:intergenic 60.0%
AAGTAGGAAACGAGGGCGGG+AGG - chr1_1:67689840-67689859 None:intergenic 60.0%
CGCACGGGGAGAAAGTTACC+AGG - chr1_1:67691214-67691233 None:intergenic 60.0%
GAAACGAGGGCGGGAGGAAG+AGG - chr1_1:67689834-67689853 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 67689529 67693152 67689529 ID=Msa0038290;Name=Msa0038290
chr1_1 mRNA 67689529 67693152 67689529 ID=Msa0038290-mRNA-1;Parent=Msa0038290;Name=Msa0038290-mRNA-1;_AED=0.00;_eAED=0.00;_QI=148|1|1|1|0.75|0.6|5|404|173
chr1_1 exon 67689529 67689865 67689529 ID=Msa0038290-mRNA-1:exon:22002;Parent=Msa0038290-mRNA-1
chr1_1 exon 67691126 67691214 67691126 ID=Msa0038290-mRNA-1:exon:22003;Parent=Msa0038290-mRNA-1
chr1_1 exon 67691379 67691556 67691379 ID=Msa0038290-mRNA-1:exon:22004;Parent=Msa0038290-mRNA-1
chr1_1 exon 67692551 67692621 67692551 ID=Msa0038290-mRNA-1:exon:22005;Parent=Msa0038290-mRNA-1
chr1_1 exon 67692754 67693152 67692754 ID=Msa0038290-mRNA-1:exon:22006;Parent=Msa0038290-mRNA-1
chr1_1 five_prime_UTR 67689529 67689676 67689529 ID=Msa0038290-mRNA-1:five_prime_utr;Parent=Msa0038290-mRNA-1
chr1_1 CDS 67689677 67689865 67689677 ID=Msa0038290-mRNA-1:cds;Parent=Msa0038290-mRNA-1
chr1_1 CDS 67691126 67691214 67691126 ID=Msa0038290-mRNA-1:cds;Parent=Msa0038290-mRNA-1
chr1_1 CDS 67691379 67691556 67691379 ID=Msa0038290-mRNA-1:cds;Parent=Msa0038290-mRNA-1
chr1_1 CDS 67692551 67692616 67692551 ID=Msa0038290-mRNA-1:cds;Parent=Msa0038290-mRNA-1
chr1_1 three_prime_UTR 67692617 67692621 67692617 ID=Msa0038290-mRNA-1:three_prime_utr;Parent=Msa0038290-mRNA-1
chr1_1 three_prime_UTR 67692754 67693152 67692754 ID=Msa0038290-mRNA-1:three_prime_utr;Parent=Msa0038290-mRNA-1
Gene Sequence

>Msa0038290

ATGGCCGCTTCCATCTCAGCAGCGTCTTCGACTGTTATCCTCCATTCTTTGAACTCTTCTTCGTCCACACGTCTGGTACCTTCATCATCATCATCATCTTCACACCTTCAATTTCGAACTCCTCGCCTTCTTCCAATTGGTACTCGCCGTCTTTCCTCTTCCTCCCGCCCTCGTTTCCTACTTCCTCTCGTTCAAGCAAAGAAACAAACCTTTTCCTCTTTTGATGATTTGCTTGCAAACTCCGACAAGCCTGTATTTGTTGACTTCTATGCAACCTGGTGTGGTCCTTGTCAGTTCATGGTTCCTGTACTTGAGGAAGTGAGCGCTCGGCTTCAAGATCAGATACAAATAGTTAAGATTGATACTGAGAAGTATCCTAGCATTGCCAACAAATACAACATTGAGGCATTGCCAACTTTCATCATCTTTAAGGATGGAAAACCCTTTGATCGCTTTGAGGGAGCATTAACAGCAGATAAGCTAATTGAACGCATAGAAACCACTCTCAACGTTAAGCAATAA
Protein sequence

>Msa0038290

MAASISAASSTVILHSLNSSSSTRLVPSSSSSSSHLQFRTPRLLPIGTRRLSSSSRPRFLLPLVQAKKQTFSSFDDLLANSDKPVFVDFYATWCGPCQFMVPVLEEVSARLQDQIQIVKIDTEKYPSIANKYNIEALPTFIIFKDGKPFDRFEGALTADKLIERIETTLNVKQ*