AlfalfaGEDB | Msa0610690

M. sativa cultivar zhongmu-4 / Msa0610690

KEGG: K02437
GO: GO:0000166 GO:0003674 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0008144 GO:0017076 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0097159 GO:0097367 GO:1901265 GO:1901363
PFAM: PF01597.22

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0610690	XP_003607890.1	98.065	155	3	0	1	155	1	155	1.12e-108	316

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0610690	sp\|O82179\|GCSH2_ARATH	78.205	156	33	1	1	155	1	156	2.76e-87	254

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0022400	Msa0610690	0.837328	5.545932e-57	-8.615850e-47
Msa0033220	Msa0610690	0.851381	9.208375e-61	-8.615850e-47
Msa0038150	Msa0610690	-0.805828	1.105427e-49	-8.615850e-47
Msa0044160	Msa0610690	0.823514	1.329853e-53	-8.615850e-47
Msa0044440	Msa0610690	0.841079	5.906122e-58	-8.615850e-47
Msa0063940	Msa0610690	0.893342	6.875300e-75	-8.615850e-47
Msa0077340	Msa0610690	0.841237	5.367564e-58	-8.615850e-47
Msa0079880	Msa0610690	0.831778	1.376196e-55	-8.615850e-47
Msa0080030	Msa0610690	0.841520	4.521248e-58	-8.615850e-47
Msa0088520	Msa0610690	0.822102	2.835143e-53	-8.615850e-47
Msa0098240	Msa0610690	0.822151	2.761746e-53	-8.615850e-47
Msa0101530	Msa0610690	0.841555	4.425446e-58	-8.615850e-47
Msa0105650	Msa0610690	0.832767	7.834594e-56	-8.615850e-47
Msa0610690	Msa0619780	0.823647	1.237580e-53	-8.615850e-47
Msa0610690	Msa0622670	0.807219	5.625649e-50	-8.615850e-47
Msa0610690	Msa0630440	0.811201	7.880782e-51	-8.615850e-47
Msa0610690	Msa0644330	0.837365	5.426246e-57	-8.615850e-47
Msa0610690	Msa0657710	0.848158	7.326752e-60	-8.615850e-47
Msa0610690	Msa0657720	0.819136	1.360584e-52	-8.615850e-47
Msa0610690	Msa0674480	0.866249	3.272237e-65	-8.615850e-47
Msa0610690	Msa0678380	0.805219	1.483808e-49	-8.615850e-47
Msa0610690	Msa0693330	0.802070	6.677756e-49	-8.615850e-47
Msa0610690	Msa0693340	-0.803683	3.100493e-49	-8.615850e-47
Msa0610690	Msa0702410	0.804602	1.997007e-49	-8.615850e-47
Msa0610690	Msa0702790	0.815020	1.144937e-51	-8.615850e-47
Msa0610690	Msa0702800	0.858018	1.098495e-62	-8.615850e-47
Msa0610690	Msa0703950	0.837257	5.784757e-57	-8.615850e-47
Msa0610690	Msa0706230	0.800327	1.518240e-48	-8.615850e-47
Msa0610690	Msa0707330	0.802613	5.163160e-49	-8.615850e-47
Msa0610690	Msa0707360	0.822462	2.338694e-53	-8.615850e-47
Msa0610690	Msa0712860	0.829647	4.578937e-55	-8.615850e-47
Msa0610690	Msa0722890	0.811275	7.593835e-51	-8.615850e-47
Msa0610690	Msa0723090	0.809477	1.856363e-50	-8.615850e-47
Msa0610690	Msa0726170	-0.833347	5.617740e-56	-8.615850e-47
Msa0610690	Msa0742370	-0.874664	5.659897e-68	-8.615850e-47
Msa0610690	Msa0746650	0.808662	2.774072e-50	-8.615850e-47
Msa0610690	Msa0758780	-0.823520	1.325376e-53	-8.615850e-47
Msa0610690	Msa0758810	-0.830352	3.081673e-55	-8.615850e-47
Msa0610690	Msa0765960	-0.819488	1.131104e-52	-8.615850e-47
Msa0610690	Msa0779450	-0.844515	7.207886e-59	-8.615850e-47
Msa0610690	Msa0792030	0.804147	2.484231e-49	-8.615850e-47
Msa0610690	Msa0795750	0.880316	6.067281e-70	-8.615850e-47
Msa0610690	Msa0800180	0.801266	9.765775e-49	-8.615850e-47
Msa0610690	Msa0800480	0.831738	1.408162e-55	-8.615850e-47
Msa0610690	Msa0805390	0.802874	4.561483e-49	-8.615850e-47
Msa0610690	Msa0811690	0.858452	8.157083e-63	-8.615850e-47
Msa0610690	Msa0823480	-0.841098	5.837904e-58	-8.615850e-47
Msa0610690	Msa0828950	0.820758	5.791922e-53	-8.615850e-47
Msa0610690	Msa0828960	0.857593	1.468322e-62	-8.615850e-47
Msa0610690	Msa0833530	0.811966	5.375095e-51	-8.615850e-47
Msa0610690	Msa0838620	-0.800735	1.253628e-48	-8.615850e-47
Msa0610690	Msa0851850	0.884269	2.214260e-71	-8.615850e-47
Msa0610690	Msa0862480	-0.871285	7.679630e-67	-8.615850e-47
Msa0610690	Msa0865420	0.805755	1.145218e-49	-8.615850e-47
Msa0610690	Msa0909260	0.825616	4.252609e-54	-8.615850e-47
Msa0610690	Msa0930770	0.811280	7.574545e-51	-8.615850e-47
Msa0610690	Msa0940910	0.836779	7.659176e-57	-8.615850e-47
Msa0610690	Msa0958830	0.811425	7.046395e-51	-8.615850e-47
Msa0610690	Msa0964020	0.812269	4.615245e-51	-8.615850e-47
Msa0610690	Msa0967070	0.806537	7.840509e-50	-8.615850e-47
Msa0610690	Msa0967170	0.821865	3.217015e-53	-8.615850e-47
Msa0610690	Msa0967780	0.802139	6.464684e-49	-8.615850e-47
Msa0610690	Msa0972680	0.804185	2.439222e-49	-8.615850e-47
Msa0610690	Msa0982730	0.802487	5.481351e-49	-8.615850e-47
Msa0610690	Msa0994720	0.803984	2.684753e-49	-8.615850e-47
Msa0610690	Msa1015360	0.852603	4.143064e-61	-8.615850e-47
Msa0610690	Msa1022420	0.851137	1.079484e-60	-8.615850e-47
Msa0610690	Msa1023090	0.801670	8.071708e-49	-8.615850e-47
Msa0610690	Msa1032470	0.836877	7.230414e-57	-8.615850e-47
Msa0610690	Msa1039100	0.830141	3.471068e-55	-8.615850e-47
Msa0610690	Msa1039440	-0.829260	5.684741e-55	-8.615850e-47
Msa0610690	Msa1041060	0.817204	3.722073e-52	-8.615850e-47
Msa0610690	Msa1044250	0.858475	8.032480e-63	-8.615850e-47
Msa0610690	Msa1082410	0.841645	4.190868e-58	-8.615850e-47
Msa0610690	Msa1083700	0.814435	1.542666e-51	-8.615850e-47
Msa0610690	Msa1094810	0.824806	6.611024e-54	-8.615850e-47
Msa0610690	Msa1106590	0.811711	6.105746e-51	-8.615850e-47
Msa0610690	Msa1114690	0.845214	4.669852e-59	-8.615850e-47
Msa0610690	Msa1128520	-0.808666	2.768921e-50	-8.615850e-47
Msa0610690	Msa1133750	0.841109	5.798010e-58	-8.615850e-47
Msa0610690	Msa1147650	0.820088	8.251129e-53	-8.615850e-47
Msa0610690	Msa1154320	0.829041	6.424691e-55	-8.615850e-47
Msa0610690	Msa1165460	0.820552	6.456360e-53	-8.615850e-47
Msa0610690	Msa1184680	0.853694	2.017272e-61	-8.615850e-47
Msa0610690	Msa1190770	0.802142	6.454938e-49	-8.615850e-47
Msa0610690	Msa1194240	0.813226	2.849721e-51	-8.615850e-47
Msa0610690	Msa1197720	0.856019	4.268154e-62	-8.615850e-47
Msa0610690	Msa1203650	0.821344	4.245479e-53	-8.615850e-47
Msa0610690	Msa1207920	-0.850652	1.478176e-60	-8.615850e-47
Msa0610690	Msa1210040	0.803612	3.208644e-49	-8.615850e-47
Msa0610690	Msa1212830	0.816667	4.913544e-52	-8.615850e-47
Msa0610690	Msa1213040	0.821406	4.105746e-53	-8.615850e-47
Msa0610690	Msa1219930	0.842385	2.670439e-58	-8.615850e-47
Msa0610690	Msa1240190	0.825846	3.750882e-54	-8.615850e-47
Msa0610690	Msa1240220	0.817891	2.606188e-52	-8.615850e-47
Msa0610690	Msa1251410	0.862048	6.677107e-64	-8.615850e-47
Msa0610690	Msa1260220	0.839521	1.507552e-57	-8.615850e-47
Msa0610690	Msa1268390	0.804796	1.819017e-49	-8.615850e-47
Msa0610690	Msa1268420	0.803890	2.808422e-49	-8.615850e-47
Msa0610690	Msa1283930	0.816618	5.038720e-52	-8.615850e-47
Msa0610690	Msa1284020	0.803341	3.650420e-49	-8.615850e-47
Msa0610690	Msa1299650	0.805736	1.155677e-49	-8.615850e-47
Msa0610690	Msa1322960	0.823393	1.418726e-53	-8.615850e-47
Msa0610690	Msa1322980	0.800637	1.312525e-48	-8.615850e-47
Msa0610690	Msa1323070	0.886630	2.892321e-72	-8.615850e-47
Msa0610690	Msa1326710	0.811169	8.009945e-51	-8.615850e-47
Msa0610690	Msa1327800	0.811482	6.848099e-51	-8.615850e-47
Msa0610690	Msa1331570	-0.842317	2.783597e-58	-8.615850e-47
Msa0610690	Msa1349690	0.815915	7.236866e-52	-8.615850e-47
Msa0610690	Msa1351990	0.810951	8.930215e-51	-8.615850e-47
Msa0610690	Msa1352580	0.829335	5.453370e-55	-8.615850e-47
Msa0610690	Msa1352600	-0.811196	7.900633e-51	-8.615850e-47
Msa0610690	Msa1358290	0.815934	7.166960e-52	-8.615850e-47
Msa0610690	Msa1368390	0.822200	2.690243e-53	-8.615850e-47
Msa0610690	Msa1381260	0.807471	4.974765e-50	-8.615850e-47
Msa0610690	Msa1384700	0.846172	2.565767e-59	-8.615850e-47
Msa0610690	Msa1389900	0.819033	1.436016e-52	-8.615850e-47
Msa0610690	Msa1391500	0.858674	7.004608e-63	-8.615850e-47
Msa0610690	Msa1392740	0.815896	7.307447e-52	-8.615850e-47
Msa0610690	Msa1396200	-0.865859	4.349276e-65	-8.615850e-47
Msa0610690	Msa1398810	0.816790	4.610696e-52	-8.615850e-47
Msa0610690	Msa1401910	0.801258	9.799867e-49	-8.615850e-47
Msa0610690	Msa1405460	0.815597	8.521532e-52	-8.615850e-47
Msa0610690	Msa1407840	0.809117	2.217438e-50	-8.615850e-47
Msa0610690	Msa1408390	0.836855	7.328621e-57	-8.615850e-47
Msa0610690	Msa1412000	0.811545	6.636235e-51	-8.615850e-47
Msa0610690	Msa1422860	0.865017	8.008061e-65	-8.615850e-47
Msa0610690	Msa1428230	-0.836211	1.068530e-56	-8.615850e-47
Msa0610690	Msa1433360	0.826752	2.282620e-54	-8.615850e-47
Msa0610690	Msa1434300	0.810876	9.268862e-51	-8.615850e-47
Msa0610690	Msa1437810	0.810522	1.105356e-50	-8.615850e-47
Msa0610690	Msa1439250	0.884552	1.739991e-71	-8.615850e-47
Msa0610690	Msa1439930	0.829918	3.934168e-55	-8.615850e-47
Msa0610690	Msa1445530	0.816714	4.795581e-52	-8.615850e-47
Msa0610690	Msa1447100	0.821915	3.131699e-53	-8.615850e-47
Msa0610690	Msa1454500	0.806751	7.066106e-50	-8.615850e-47
Msa0610690	Msa1457770	0.805355	1.389634e-49	-8.615850e-47
Msa0610690	Msa1457890	0.806280	8.879669e-50	-8.615850e-47
Msa0610690	Msa1464880	0.802957	4.384612e-49	-8.615850e-47
Msa0125360	Msa0610690	0.820518	6.575320e-53	-8.615850e-47
Msa0127770	Msa0610690	0.811622	6.384184e-51	-8.615850e-47
Msa0133140	Msa0610690	-0.801273	9.733324e-49	-8.615850e-47
Msa0139500	Msa0610690	0.801142	1.035134e-48	-8.615850e-47
Msa0142430	Msa0610690	0.859581	3.745806e-63	-8.615850e-47
Msa0152850	Msa0610690	0.812103	5.016584e-51	-8.615850e-47
Msa0161480	Msa0610690	0.817534	3.136796e-52	-8.615850e-47
Msa0165640	Msa0610690	0.845418	4.111102e-59	-8.615850e-47
Msa0183310	Msa0610690	-0.830471	2.882976e-55	-8.615850e-47
Msa0183710	Msa0610690	0.834094	3.655980e-56	-8.615850e-47
Msa0189270	Msa0610690	0.809415	1.913608e-50	-8.615850e-47
Msa0189560	Msa0610690	-0.804807	1.809351e-49	-8.615850e-47
Msa0192250	Msa0610690	0.805211	1.489531e-49	-8.615850e-47
Msa0195230	Msa0610690	0.803970	2.703992e-49	-8.615850e-47
Msa0204950	Msa0610690	0.819691	1.016775e-52	-8.615850e-47
Msa0217060	Msa0610690	0.813580	2.381852e-51	-8.615850e-47
Msa0219280	Msa0610690	0.825697	4.070037e-54	-8.615850e-47
Msa0365210	Msa0610690	0.841581	4.357095e-58	-8.615850e-47
Msa0376430	Msa0610690	0.809066	2.273917e-50	-8.615850e-47
Msa0384590	Msa0610690	0.853317	2.589264e-61	-8.615850e-47
Msa0397130	Msa0610690	0.809241	2.085123e-50	-8.615850e-47
Msa0402070	Msa0610690	-0.861806	7.918629e-64	-8.615850e-47
Msa0412790	Msa0610690	0.810068	1.385396e-50	-8.615850e-47
Msa0434660	Msa0610690	0.841616	4.264631e-58	-8.615850e-47
Msa0436440	Msa0610690	0.815084	1.108030e-51	-8.615850e-47
Msa0437440	Msa0610690	-0.856670	2.749762e-62	-8.615850e-47
Msa0437520	Msa0610690	0.821236	4.495629e-53	-8.615850e-47
Msa0478430	Msa0610690	0.821680	3.549888e-53	-8.615850e-47
Msa0480900	Msa0610690	-0.822835	1.915365e-53	-8.615850e-47
Msa0256920	Msa0610690	0.813062	3.094702e-51	-8.615850e-47
Msa0281470	Msa0610690	0.855339	6.742790e-62	-8.615850e-47
Msa0282960	Msa0610690	0.805035	1.620900e-49	-8.615850e-47
Msa0286630	Msa0610690	0.807909	4.015316e-50	-8.615850e-47
Msa0288620	Msa0610690	0.822278	2.579981e-53	-8.615850e-47
Msa0313900	Msa0610690	0.827583	1.444255e-54	-8.615850e-47
Msa0320600	Msa0610690	0.813802	2.128603e-51	-8.615850e-47
Msa0330520	Msa0610690	0.828350	9.441227e-55	-8.615850e-47
Msa0334660	Msa0610690	0.807029	6.172164e-50	-8.615850e-47
Msa0336120	Msa0610690	0.804359	2.243517e-49	-8.615850e-47
Msa0354970	Msa0610690	-0.847999	8.105389e-60	-8.615850e-47
Msa0355080	Msa0610690	-0.876762	1.078890e-68	-8.615850e-47
Msa0516250	Msa0610690	0.802560	5.295676e-49	-8.615850e-47
Msa0522540	Msa0610690	0.854228	1.415759e-61	-8.615850e-47
Msa0526150	Msa0610690	0.849200	3.767836e-60	-8.615850e-47
Msa0529030	Msa0610690	0.811965	5.375544e-51	-8.615850e-47
Msa0543560	Msa0610690	0.833427	5.367108e-56	-8.615850e-47
Msa0566280	Msa0610690	0.821203	4.575302e-53	-8.615850e-47
Msa0566940	Msa0610690	-0.824376	8.345895e-54	-8.615850e-47
Msa0567330	Msa0610690	0.817904	2.588865e-52	-8.615850e-47
Msa0576320	Msa0610690	0.803505	3.376972e-49	-8.615850e-47
Msa0576790	Msa0610690	0.810730	9.967488e-51	-8.615850e-47
Msa0577860	Msa0610690	-0.808501	3.002914e-50	-8.615850e-47
Msa0578830	Msa0610690	0.801741	7.801947e-49	-8.615850e-47
Msa0579080	Msa0610690	0.940793	1.269391e-100	-8.615850e-47
Msa0579160	Msa0610690	0.970354	1.727371e-131	-8.615850e-47
Msa0579220	Msa0610690	0.969333	5.714777e-130	-8.615850e-47
Msa0590620	Msa0610690	-0.843190	1.631737e-58	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0610690	MtrunA17_Chr4g0043711	98.065	155	3	0	1	155	1	155	1.03e-112	316
Msa0610690	MtrunA17_Chr5g0400431	66.418	134	45	0	22	155	33	166	2.25e-66	199
Msa0610690	MtrunA17_Chr7g0260591	77.358	53	10	1	53	103	3	55	5.48e-20	78.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0610690	AT2G35120.1	78.205	156	33	1	1	155	1	156	2.81e-88	254
Msa0610690	AT1G32470.1	58.084	167	57	2	1	155	1	166	8.19e-63	191
Msa0610690	AT2G35370.1	65.441	136	47	0	20	155	30	165	1.88e-62	189

Find 53 sgRNAs with CRISPR-Local

Find 261 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GACATCAACTCTCCTGTTTC+AGG	0.209182	4_2:+58533708	Msa0610690:CDS
TTCACTGCCGTTCCCACTTC+TGG	0.241034	4_2:-58533639	None:intergenic
AACTCAAGCCCCTATGAAAA+TGG	0.268193	4_2:+58533900	Msa0610690:CDS
GAATGAATGAATGAAATTAT+TGG	0.292964	4_2:-58532149	Msa0610680:intron
AGATGGCTTGCAGGCAGTTA+TGG	0.326829	4_2:+58532214	Msa0610690:exon
AGTGAAGAGTGGTAGGGTTA+AGG	0.337032	4_2:-58532079	Msa0610680:intron
TGATCATGCCCAAGATCATT+TGG	0.340864	4_2:+58533593	Msa0610690:CDS
TGGTATAGATGATGTCAATT+TGG	0.362666	4_2:+58534058	Msa0610690:three_prime_UTR
GTGAAACAAGGAGATGGTTT+TGG	0.366878	4_2:+58533657	Msa0610690:CDS
ATCTTCTTCTTCACAGAACT+TGG	0.368009	4_2:-58533991	None:intergenic
CAACATCACCCAAATGATCT+TGG	0.387609	4_2:-58533602	None:intergenic
GATGGCTTGCAGGCAGTTAT+GGG	0.405390	4_2:+58532215	Msa0610690:exon
AGTGGTAGGGTTAAGGAAGT+TGG	0.419936	4_2:-58532072	Msa0610680:intron
GAGAGAGAGTGAAGAGTGGT+AGG	0.424407	4_2:-58532086	Msa0610680:intron
TGGCGAACTTGACAAATTGA+TGG	0.434808	4_2:+58533953	Msa0610690:CDS
GCTCAGCAACTCTCCTGCTT+TGG	0.435121	4_2:+58533755	Msa0610690:CDS
CCTGAGGTATGATGCGGCTC+TGG	0.441316	4_2:-58532241	Msa0610680:intron
ATTCATTCATTCAGTGTAAG+TGG	0.444370	4_2:+58532160	Msa0610690:five_prime_UTR
TGAATGTCGTATGACAAAAC+TGG	0.478972	4_2:-58534261	None:intergenic
GATCATGCCCAAGATCATTT+GGG	0.482133	4_2:+58533594	Msa0610690:CDS
TGGGAAGTACTGAAAGATAT+CGG	0.488163	4_2:-58534034	None:intergenic
GCAATAAAACTCACCAAAGC+AGG	0.490891	4_2:-58533768	None:intergenic
CATGAATGGGTGAAAGTTGA+CGG	0.492157	4_2:+58533546	Msa0610690:CDS
AACATCACCCAAATGATCTT+GGG	0.497081	4_2:-58533601	None:intergenic
CCAGAGCCGCATCATACCTC+AGG	0.521213	4_2:+58532241	Msa0610690:CDS
AGTATGCTGATTCTCATGAA+TGG	0.523325	4_2:+58533532	Msa0610690:CDS
TGCGCTAGCTTCTGATGCAG+AGG	0.534256	4_2:+58534139	Msa0610690:three_prime_UTR
TTGACATCATCTATACCAAT+GGG	0.535199	4_2:-58534053	None:intergenic
CAAGCCCCTATGAAAATGGA+TGG	0.537218	4_2:+58533904	Msa0610690:CDS
TATCTTTCAGTACTTCCCAT+TGG	0.548271	4_2:+58534038	Msa0610690:three_prime_UTR
GCGCTAGCTTCTGATGCAGA+GGG	0.548373	4_2:+58534140	Msa0610690:three_prime_UTR
CTGAGAGAGAGAGTGAAGAG+TGG	0.551470	4_2:-58532090	Msa0610680:intron
GTATGCTGATTCTCATGAAT+GGG	0.551627	4_2:+58533533	Msa0610690:CDS
AGAGAGAGTGAAGAGTGGTA+GGG	0.552288	4_2:-58532085	Msa0610680:intron
AAACTAAAATGAATGTCGTA+TGG	0.554404	4_2:-58534297	None:intergenic
ATTGACATCATCTATACCAA+TGG	0.555452	4_2:-58534054	None:intergenic
GACGGAAAATCTGCAACCAT+TGG	0.558598	4_2:+58533564	Msa0610690:CDS
TCGGCAAGACTATCAATGAC+TGG	0.570070	4_2:-58534015	None:intergenic
ATTGCTGTGGTTGGTGACAA+AGG	0.572500	4_2:-58532116	Msa0610680:intron
TTTCACAGAACCTTCTCTAA+CGG	0.580128	4_2:+58532273	Msa0610690:CDS
TTGCTGTGGTTGGTGACAAA+GGG	0.582432	4_2:-58532115	Msa0610680:intron
TGAATGAATACCGTTAGAGA+AGG	0.619094	4_2:-58532283	Msa0610680:CDS
TGGTGCAGTTGAAAGTGTGA+AGG	0.619953	4_2:+58533677	Msa0610690:CDS
TGAATTGCCAGAAGTGGGAA+CGG	0.630383	4_2:+58533632	Msa0610690:CDS
GTGGGAACGGCAGTGAAACA+AGG	0.631106	4_2:+58533645	Msa0610690:CDS
ACGGCAGTGAAACAAGGAGA+TGG	0.634473	4_2:+58533651	Msa0610690:CDS
GGCATGATCAGTTATGCCAA+TGG	0.640158	4_2:-58533580	None:intergenic
CATTCATTCAGTGTAAGTGG+TGG	0.640220	4_2:+58532163	Msa0610690:five_prime_UTR
GTATGTTGAATTGCCAGAAG+TGG	0.646780	4_2:+58533626	Msa0610690:CDS
TGTGAAAGACAGAGATCCTG+AGG	0.655630	4_2:-58532257	Msa0610680:intron
TATGTTGAATTGCCAGAAGT+GGG	0.663199	4_2:+58533627	Msa0610690:CDS
AGAGATCCTGAGGTATGATG+CGG	0.690860	4_2:-58532247	Msa0610680:intron
AAAGTTGAGATGAGTGACAG+TGG	0.702478	4_2:+58533933	Msa0610690:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATAAAAGTTAATTATGAATA+AGG	-	chr4_2:58533793-58533812	None:intergenic	10.0%
!!	ATAAAAGTTAATTATGAATA+AGG	-	chr4_2:58533793-58533812	None:intergenic	10.0%
!!	TAATAATAAAATAAAAAGCG+AGG	-	chr4_2:58532388-58532407	None:intergenic	15.0%
!!	TTGTTAACAAAAAAACAAAA+TGG	-	chr4_2:58532618-58532637	None:intergenic	15.0%
!!!	AGATTTTATTGAGATATTAA+CGG	-	chr4_2:58533087-58533106	None:intergenic	15.0%
!!!	AACCAAAATTTATAATTTGT+AGG	+	chr4_2:58533235-58533254	Msa0610690:intron	15.0%
!!!	ATCCTACAAATTATAAATTT+TGG	-	chr4_2:58533240-58533259	None:intergenic	15.0%
!!!	AAAATTTATAATTTGTAGGA+TGG	+	chr4_2:58533239-58533258	Msa0610690:intron	15.0%
!!	TTAAAATTCAATATGCAAAT+TGG	-	chr4_2:58533285-58533304	None:intergenic	15.0%
!!!	TTTTAATATCTTGAAAGTTT+TGG	+	chr4_2:58533299-58533318	Msa0610690:intron	15.0%
!!	TAATAATAAAATAAAAAGCG+AGG	-	chr4_2:58532388-58532407	None:intergenic	15.0%
!!	TTGTTAACAAAAAAACAAAA+TGG	-	chr4_2:58532618-58532637	None:intergenic	15.0%
!!!	AGATTTTATTGAGATATTAA+CGG	-	chr4_2:58533087-58533106	None:intergenic	15.0%
!!!	AACCAAAATTTATAATTTGT+AGG	+	chr4_2:58533235-58533254	Msa0610690:intron	15.0%
!!!	ATCCTACAAATTATAAATTT+TGG	-	chr4_2:58533240-58533259	None:intergenic	15.0%
!!!	AAAATTTATAATTTGTAGGA+TGG	+	chr4_2:58533239-58533258	Msa0610690:intron	15.0%
!!	TTAAAATTCAATATGCAAAT+TGG	-	chr4_2:58533285-58533304	None:intergenic	15.0%
!!!	TTTTAATATCTTGAAAGTTT+TGG	+	chr4_2:58533299-58533318	Msa0610690:intron	15.0%
!!	GAATGAATGAATGAAATTAT+TGG	-	chr4_2:58532152-58532171	Msa0610680:intron	20.0%
!!	AGATCAAGTTCAAAATAAAT+TGG	-	chr4_2:58532486-58532505	None:intergenic	20.0%
!!	ATATGTAAGTAACATATACT+AGG	+	chr4_2:58532637-58532656	Msa0610690:intron	20.0%
!!	AAAAATGTAACAAACAACAT+AGG	-	chr4_2:58532716-58532735	None:intergenic	20.0%
!!!	AAATGTTTGAATAGCTTTAT+AGG	-	chr4_2:58532986-58533005	None:intergenic	20.0%
!!!	ATTTTGTTCTAACTATTATC+TGG	+	chr4_2:58533119-58533138	Msa0610690:intron	20.0%
!!	GAATGAATGAATGAAATTAT+TGG	-	chr4_2:58532152-58532171	Msa0610680:intron	20.0%
!!	AGATCAAGTTCAAAATAAAT+TGG	-	chr4_2:58532486-58532505	None:intergenic	20.0%
!!	ATATGTAAGTAACATATACT+AGG	+	chr4_2:58532637-58532656	Msa0610690:intron	20.0%
!!	AAAAATGTAACAAACAACAT+AGG	-	chr4_2:58532716-58532735	None:intergenic	20.0%
!!!	AAATGTTTGAATAGCTTTAT+AGG	-	chr4_2:58532986-58533005	None:intergenic	20.0%
!!!	ATTTTGTTCTAACTATTATC+TGG	+	chr4_2:58533119-58533138	Msa0610690:intron	20.0%
!	TCAAGTTCAAAATAAATTGG+AGG	-	chr4_2:58532483-58532502	None:intergenic	25.0%
!!	TTTTAGTGAATAGTGACAAA+AGG	+	chr4_2:58532586-58532605	Msa0610690:intron	25.0%
!!	AGAATATTCCATTTCCATTT+TGG	-	chr4_2:58532670-58532689	None:intergenic	25.0%
!!!	AAGTTCGTTTTTGCTTTTAA+AGG	+	chr4_2:58532835-58532854	Msa0610690:intron	25.0%
!	ATAACTCAGTCAAAAGAAAT+TGG	-	chr4_2:58532950-58532969	None:intergenic	25.0%
!!!	GAATAGCTTTATAGGTAAAT+TGG	-	chr4_2:58532978-58532997	None:intergenic	25.0%
!!	TAACGGTTTAATTGATTTCA+AGG	-	chr4_2:58533070-58533089	None:intergenic	25.0%
!	TATTATCTGGTACATCATAT+TGG	+	chr4_2:58533132-58533151	Msa0610690:intron	25.0%
!	TTAAAACTTAAAGTGAACGA+TGG	+	chr4_2:58533166-58533185	Msa0610690:intron	25.0%
!	TAAAACTTAAAGTGAACGAT+GGG	+	chr4_2:58533167-58533186	Msa0610690:intron	25.0%
!	CTTACAAATGAACAATGATA+TGG	-	chr4_2:58533198-58533217	None:intergenic	25.0%
!	TAGGAAAACATAAAAACACT+TGG	-	chr4_2:58533862-58533881	None:intergenic	25.0%
!!	ATTATCCATCCATTTTCATA+GGG	-	chr4_2:58533912-58533931	None:intergenic	25.0%
!!	AATTATCCATCCATTTTCAT+AGG	-	chr4_2:58533913-58533932	None:intergenic	25.0%
!!	AAAAATGAGATTGATAACCT+GGG	+	chr4_2:58534188-58534207	Msa0610690:three_prime_UTR	25.0%
!!	TTTTTATTATCAAACTTCCC+AGG	-	chr4_2:58534208-58534227	None:intergenic	25.0%
!	AAACTAAAATGAATGTCGTA+TGG	-	chr4_2:58534273-58534292	None:intergenic	25.0%
!	TCAAGTTCAAAATAAATTGG+AGG	-	chr4_2:58532483-58532502	None:intergenic	25.0%
!!	TTTTAGTGAATAGTGACAAA+AGG	+	chr4_2:58532586-58532605	Msa0610690:intron	25.0%
!!	AGAATATTCCATTTCCATTT+TGG	-	chr4_2:58532670-58532689	None:intergenic	25.0%
!!!	AAGTTCGTTTTTGCTTTTAA+AGG	+	chr4_2:58532835-58532854	Msa0610690:intron	25.0%
!	ATAACTCAGTCAAAAGAAAT+TGG	-	chr4_2:58532950-58532969	None:intergenic	25.0%
!!!	GAATAGCTTTATAGGTAAAT+TGG	-	chr4_2:58532978-58532997	None:intergenic	25.0%
!!	TAACGGTTTAATTGATTTCA+AGG	-	chr4_2:58533070-58533089	None:intergenic	25.0%
!	TATTATCTGGTACATCATAT+TGG	+	chr4_2:58533132-58533151	Msa0610690:intron	25.0%
!	TTAAAACTTAAAGTGAACGA+TGG	+	chr4_2:58533166-58533185	Msa0610690:intron	25.0%
!	TAAAACTTAAAGTGAACGAT+GGG	+	chr4_2:58533167-58533186	Msa0610690:intron	25.0%
!	CTTACAAATGAACAATGATA+TGG	-	chr4_2:58533198-58533217	None:intergenic	25.0%
!	TAGGAAAACATAAAAACACT+TGG	-	chr4_2:58533862-58533881	None:intergenic	25.0%
!!	ATTATCCATCCATTTTCATA+GGG	-	chr4_2:58533912-58533931	None:intergenic	25.0%
!!	AATTATCCATCCATTTTCAT+AGG	-	chr4_2:58533913-58533932	None:intergenic	25.0%
!!	AAAAATGAGATTGATAACCT+GGG	+	chr4_2:58534188-58534207	Msa0610690:three_prime_UTR	25.0%
!!	TTTTTATTATCAAACTTCCC+AGG	-	chr4_2:58534208-58534227	None:intergenic	25.0%
!	AAACTAAAATGAATGTCGTA+TGG	-	chr4_2:58534273-58534292	None:intergenic	25.0%
!!!	CTTTTTTATATTGCTGTGGT+TGG	-	chr4_2:58532128-58532147	Msa0610680:intron	30.0%
	CAACCACAGCAATATAAAAA+AGG	+	chr4_2:58532126-58532145	Msa0610690:five_prime_UTR	30.0%
	ATTCATTCATTCAGTGTAAG+TGG	+	chr4_2:58532160-58532179	Msa0610690:five_prime_UTR	30.0%
	ATTGCCTTTATATACGTTAG+TGG	+	chr4_2:58532423-58532442	Msa0610690:intron	30.0%
	TTGCCTTTATATACGTTAGT+GGG	+	chr4_2:58532424-58532443	Msa0610690:intron	30.0%
!	TTTTGAACTTGATCTGTATG+TGG	+	chr4_2:58532491-58532510	Msa0610690:intron	30.0%
	AATTGAAAGAATAGATCGTC+CGG	-	chr4_2:58532568-58532587	None:intergenic	30.0%
	TACTAGGCTAAATTCCAAAA+TGG	+	chr4_2:58532653-58532672	Msa0610690:intron	30.0%
	GCTAAATTCCAAAATGGAAA+TGG	+	chr4_2:58532659-58532678	Msa0610690:intron	30.0%
	GTGCATATTTCGAAATTAAG+TGG	+	chr4_2:58532793-58532812	Msa0610690:intron	30.0%
!!	AATTTAGCTTGTTAGCACTT+TGG	+	chr4_2:58532872-58532891	Msa0610690:intron	30.0%
!!	ATTTAGCTTGTTAGCACTTT+GGG	+	chr4_2:58532873-58532892	Msa0610690:intron	30.0%
!!	TAACAAACTGTTGGTTTGTT+TGG	+	chr4_2:58532902-58532921	Msa0610690:intron	30.0%
	AATAATCCAAATTACTCTCG+AGG	+	chr4_2:58533009-58533028	Msa0610690:intron	30.0%
!	CAATATGCTACTTGTTCAAT+TGG	-	chr4_2:58533412-58533431	None:intergenic	30.0%
	CAATTGAACAAGTAGCATAT+TGG	+	chr4_2:58533410-58533429	Msa0610690:intron	30.0%
!	ATTGAAACCAACATTTTGCA+AGG	+	chr4_2:58533460-58533479	Msa0610690:intron	30.0%
!!	TTTCCTATTTTTCTTCGTGT+AGG	+	chr4_2:58533875-58533894	Msa0610690:intron	30.0%
!	TTATCCATCCATTTTCATAG+GGG	-	chr4_2:58533911-58533930	None:intergenic	30.0%
	TTGACATCATCTATACCAAT+GGG	-	chr4_2:58534056-58534075	None:intergenic	30.0%
	ATTGACATCATCTATACCAA+TGG	-	chr4_2:58534057-58534076	None:intergenic	30.0%
	TGGTATAGATGATGTCAATT+TGG	+	chr4_2:58534058-58534077	Msa0610690:three_prime_UTR	30.0%
!!	TCTCATTTTTCCATTGCAAA+GGG	-	chr4_2:58534178-58534197	None:intergenic	30.0%
!!	ATCTCATTTTTCCATTGCAA+AGG	-	chr4_2:58534179-58534198	None:intergenic	30.0%
!	GAAAAATGAGATTGATAACC+TGG	+	chr4_2:58534187-58534206	Msa0610690:three_prime_UTR	30.0%
!!!	CTTTTTTATATTGCTGTGGT+TGG	-	chr4_2:58532128-58532147	Msa0610680:intron	30.0%
	CAACCACAGCAATATAAAAA+AGG	+	chr4_2:58532126-58532145	Msa0610690:five_prime_UTR	30.0%
	ATTCATTCATTCAGTGTAAG+TGG	+	chr4_2:58532160-58532179	Msa0610690:five_prime_UTR	30.0%
	ATTGCCTTTATATACGTTAG+TGG	+	chr4_2:58532423-58532442	Msa0610690:intron	30.0%
	TTGCCTTTATATACGTTAGT+GGG	+	chr4_2:58532424-58532443	Msa0610690:intron	30.0%
!	TTTTGAACTTGATCTGTATG+TGG	+	chr4_2:58532491-58532510	Msa0610690:intron	30.0%
	AATTGAAAGAATAGATCGTC+CGG	-	chr4_2:58532568-58532587	None:intergenic	30.0%
	TACTAGGCTAAATTCCAAAA+TGG	+	chr4_2:58532653-58532672	Msa0610690:intron	30.0%
	GCTAAATTCCAAAATGGAAA+TGG	+	chr4_2:58532659-58532678	Msa0610690:intron	30.0%
	GTGCATATTTCGAAATTAAG+TGG	+	chr4_2:58532793-58532812	Msa0610690:intron	30.0%
!!	AATTTAGCTTGTTAGCACTT+TGG	+	chr4_2:58532872-58532891	Msa0610690:intron	30.0%
!!	ATTTAGCTTGTTAGCACTTT+GGG	+	chr4_2:58532873-58532892	Msa0610690:intron	30.0%
!!	TAACAAACTGTTGGTTTGTT+TGG	+	chr4_2:58532902-58532921	Msa0610690:intron	30.0%
	AATAATCCAAATTACTCTCG+AGG	+	chr4_2:58533009-58533028	Msa0610690:intron	30.0%
!	CAATATGCTACTTGTTCAAT+TGG	-	chr4_2:58533412-58533431	None:intergenic	30.0%
	CAATTGAACAAGTAGCATAT+TGG	+	chr4_2:58533410-58533429	Msa0610690:intron	30.0%
!	ATTGAAACCAACATTTTGCA+AGG	+	chr4_2:58533460-58533479	Msa0610690:intron	30.0%
!!	TTTCCTATTTTTCTTCGTGT+AGG	+	chr4_2:58533875-58533894	Msa0610690:intron	30.0%
!	TTATCCATCCATTTTCATAG+GGG	-	chr4_2:58533911-58533930	None:intergenic	30.0%
	TTGACATCATCTATACCAAT+GGG	-	chr4_2:58534056-58534075	None:intergenic	30.0%
	ATTGACATCATCTATACCAA+TGG	-	chr4_2:58534057-58534076	None:intergenic	30.0%
	TGGTATAGATGATGTCAATT+TGG	+	chr4_2:58534058-58534077	Msa0610690:three_prime_UTR	30.0%
!!	TCTCATTTTTCCATTGCAAA+GGG	-	chr4_2:58534178-58534197	None:intergenic	30.0%
!!	ATCTCATTTTTCCATTGCAA+AGG	-	chr4_2:58534179-58534198	None:intergenic	30.0%
!	GAAAAATGAGATTGATAACC+TGG	+	chr4_2:58534187-58534206	Msa0610690:three_prime_UTR	30.0%
!!!	TGGCCTTTTTTATATTGCTG+TGG	-	chr4_2:58532132-58532151	Msa0610680:intron	35.0%
	TTTCACAGAACCTTCTCTAA+CGG	+	chr4_2:58532273-58532292	Msa0610690:CDS	35.0%
	TGAATGAATACCGTTAGAGA+AGG	-	chr4_2:58532286-58532305	None:intergenic	35.0%
	CATGTTGTTGATCCAATATC+TGG	+	chr4_2:58532347-58532366	Msa0610690:intron	35.0%
!	TTGATCCAATATCTGGAATC+TGG	+	chr4_2:58532354-58532373	Msa0610690:intron	35.0%
	AAGATCCAGATTCCAGATAT+TGG	-	chr4_2:58532362-58532381	None:intergenic	35.0%
	CTTCCCACTAACGTATATAA+AGG	-	chr4_2:58532430-58532449	None:intergenic	35.0%
	CTTTATATACGTTAGTGGGA+AGG	+	chr4_2:58532428-58532447	Msa0610690:intron	35.0%
	ATAAATTGGAGGTGACTGAA+TGG	-	chr4_2:58532472-58532491	None:intergenic	35.0%
!!	TTTAGCTTGTTAGCACTTTG+GGG	+	chr4_2:58532874-58532893	Msa0610690:intron	35.0%
	ATGTTTCCTCGAGAGTAATT+TGG	-	chr4_2:58533018-58533037	None:intergenic	35.0%
!	CGGTTTAATTGATTTCAAGG+AGG	-	chr4_2:58533067-58533086	None:intergenic	35.0%
	CATACAACCTTGCAAAATGT+TGG	-	chr4_2:58533470-58533489	None:intergenic	35.0%
!!	AGTATGCTGATTCTCATGAA+TGG	+	chr4_2:58533532-58533551	Msa0610690:CDS	35.0%
!!	GTATGCTGATTCTCATGAAT+GGG	+	chr4_2:58533533-58533552	Msa0610690:CDS	35.0%
	AACATCACCCAAATGATCTT+GGG	-	chr4_2:58533604-58533623	None:intergenic	35.0%
	TATGTTGAATTGCCAGAAGT+GGG	+	chr4_2:58533627-58533646	Msa0610690:CDS	35.0%
!!	TCAATCACTTTTCCTGAAAC+AGG	-	chr4_2:58533723-58533742	None:intergenic	35.0%
	TGACCTACACGAAGAAAAAT+AGG	-	chr4_2:58533881-58533900	None:intergenic	35.0%
	ATCTTCTTCTTCACAGAACT+TGG	-	chr4_2:58533994-58534013	None:intergenic	35.0%
!!	TGGGAAGTACTGAAAGATAT+CGG	-	chr4_2:58534037-58534056	None:intergenic	35.0%
	TATCTTTCAGTACTTCCCAT+TGG	+	chr4_2:58534038-58534057	Msa0610690:three_prime_UTR	35.0%
!!	TCATTTTTCCATTGCAAAGG+GGG	-	chr4_2:58534176-58534195	None:intergenic	35.0%
!!	CTCATTTTTCCATTGCAAAG+GGG	-	chr4_2:58534177-58534196	None:intergenic	35.0%
	TGAATGTCGTATGACAAAAC+TGG	-	chr4_2:58534264-58534283	None:intergenic	35.0%
!!!	TGGCCTTTTTTATATTGCTG+TGG	-	chr4_2:58532132-58532151	Msa0610680:intron	35.0%
	TTTCACAGAACCTTCTCTAA+CGG	+	chr4_2:58532273-58532292	Msa0610690:CDS	35.0%
	TGAATGAATACCGTTAGAGA+AGG	-	chr4_2:58532286-58532305	None:intergenic	35.0%
	CATGTTGTTGATCCAATATC+TGG	+	chr4_2:58532347-58532366	Msa0610690:intron	35.0%
!	TTGATCCAATATCTGGAATC+TGG	+	chr4_2:58532354-58532373	Msa0610690:intron	35.0%
	AAGATCCAGATTCCAGATAT+TGG	-	chr4_2:58532362-58532381	None:intergenic	35.0%
	CTTCCCACTAACGTATATAA+AGG	-	chr4_2:58532430-58532449	None:intergenic	35.0%
	CTTTATATACGTTAGTGGGA+AGG	+	chr4_2:58532428-58532447	Msa0610690:intron	35.0%
	ATAAATTGGAGGTGACTGAA+TGG	-	chr4_2:58532472-58532491	None:intergenic	35.0%
!!	TTTAGCTTGTTAGCACTTTG+GGG	+	chr4_2:58532874-58532893	Msa0610690:intron	35.0%
	ATGTTTCCTCGAGAGTAATT+TGG	-	chr4_2:58533018-58533037	None:intergenic	35.0%
!	CGGTTTAATTGATTTCAAGG+AGG	-	chr4_2:58533067-58533086	None:intergenic	35.0%
	CATACAACCTTGCAAAATGT+TGG	-	chr4_2:58533470-58533489	None:intergenic	35.0%
!!	AGTATGCTGATTCTCATGAA+TGG	+	chr4_2:58533532-58533551	Msa0610690:CDS	35.0%
!!	GTATGCTGATTCTCATGAAT+GGG	+	chr4_2:58533533-58533552	Msa0610690:CDS	35.0%
	AACATCACCCAAATGATCTT+GGG	-	chr4_2:58533604-58533623	None:intergenic	35.0%
	TATGTTGAATTGCCAGAAGT+GGG	+	chr4_2:58533627-58533646	Msa0610690:CDS	35.0%
!!	TCAATCACTTTTCCTGAAAC+AGG	-	chr4_2:58533723-58533742	None:intergenic	35.0%
	TGACCTACACGAAGAAAAAT+AGG	-	chr4_2:58533881-58533900	None:intergenic	35.0%
	ATCTTCTTCTTCACAGAACT+TGG	-	chr4_2:58533994-58534013	None:intergenic	35.0%
!!	TGGGAAGTACTGAAAGATAT+CGG	-	chr4_2:58534037-58534056	None:intergenic	35.0%
	TATCTTTCAGTACTTCCCAT+TGG	+	chr4_2:58534038-58534057	Msa0610690:three_prime_UTR	35.0%
!!	TCATTTTTCCATTGCAAAGG+GGG	-	chr4_2:58534176-58534195	None:intergenic	35.0%
!!	CTCATTTTTCCATTGCAAAG+GGG	-	chr4_2:58534177-58534196	None:intergenic	35.0%
	TGAATGTCGTATGACAAAAC+TGG	-	chr4_2:58534264-58534283	None:intergenic	35.0%
	CATTCATTCAGTGTAAGTGG+TGG	+	chr4_2:58532163-58532182	Msa0610690:five_prime_UTR	40.0%
	TGAATGGTGTAGTGCACTTT+CGG	-	chr4_2:58532456-58532475	None:intergenic	40.0%
!!	CACCAAAAGTTGATATACGG+CGG	-	chr4_2:58532545-58532564	None:intergenic	40.0%
!!	CGGCACCAAAAGTTGATATA+CGG	-	chr4_2:58532548-58532567	None:intergenic	40.0%
!!	CGTATATCAACTTTTGGTGC+CGG	+	chr4_2:58532546-58532565	Msa0610690:intron	40.0%
!!	TTAGCTTGTTAGCACTTTGG+GGG	+	chr4_2:58532875-58532894	Msa0610690:intron	40.0%
	GGGGGAAAATAACAAACTGT+TGG	+	chr4_2:58532893-58532912	Msa0610690:intron	40.0%
	CATGAATGGGTGAAAGTTGA+CGG	+	chr4_2:58533546-58533565	Msa0610690:CDS	40.0%
	TGATCATGCCCAAGATCATT+TGG	+	chr4_2:58533593-58533612	Msa0610690:CDS	40.0%
	GATCATGCCCAAGATCATTT+GGG	+	chr4_2:58533594-58533613	Msa0610690:CDS	40.0%
	CAACATCACCCAAATGATCT+TGG	-	chr4_2:58533605-58533624	None:intergenic	40.0%
	GTATGTTGAATTGCCAGAAG+TGG	+	chr4_2:58533626-58533645	Msa0610690:CDS	40.0%
!	GTGAAACAAGGAGATGGTTT+TGG	+	chr4_2:58533657-58533676	Msa0610690:CDS	40.0%
	GCAATAAAACTCACCAAAGC+AGG	-	chr4_2:58533771-58533790	None:intergenic	40.0%
	AACTCAAGCCCCTATGAAAA+TGG	+	chr4_2:58533900-58533919	Msa0610690:CDS	40.0%
	AAAGTTGAGATGAGTGACAG+TGG	+	chr4_2:58533933-58533952	Msa0610690:CDS	40.0%
	TGGCGAACTTGACAAATTGA+TGG	+	chr4_2:58533953-58533972	Msa0610690:CDS	40.0%
!	GATATTTTCCCCCTTTGCAA+TGG	+	chr4_2:58534165-58534184	Msa0610690:three_prime_UTR	40.0%
	CATTCATTCAGTGTAAGTGG+TGG	+	chr4_2:58532163-58532182	Msa0610690:five_prime_UTR	40.0%
	TGAATGGTGTAGTGCACTTT+CGG	-	chr4_2:58532456-58532475	None:intergenic	40.0%
!!	CACCAAAAGTTGATATACGG+CGG	-	chr4_2:58532545-58532564	None:intergenic	40.0%
!!	CGGCACCAAAAGTTGATATA+CGG	-	chr4_2:58532548-58532567	None:intergenic	40.0%
!!	CGTATATCAACTTTTGGTGC+CGG	+	chr4_2:58532546-58532565	Msa0610690:intron	40.0%
!!	TTAGCTTGTTAGCACTTTGG+GGG	+	chr4_2:58532875-58532894	Msa0610690:intron	40.0%
	GGGGGAAAATAACAAACTGT+TGG	+	chr4_2:58532893-58532912	Msa0610690:intron	40.0%
	CATGAATGGGTGAAAGTTGA+CGG	+	chr4_2:58533546-58533565	Msa0610690:CDS	40.0%
	TGATCATGCCCAAGATCATT+TGG	+	chr4_2:58533593-58533612	Msa0610690:CDS	40.0%
	GATCATGCCCAAGATCATTT+GGG	+	chr4_2:58533594-58533613	Msa0610690:CDS	40.0%
	CAACATCACCCAAATGATCT+TGG	-	chr4_2:58533605-58533624	None:intergenic	40.0%
	GTATGTTGAATTGCCAGAAG+TGG	+	chr4_2:58533626-58533645	Msa0610690:CDS	40.0%
!	GTGAAACAAGGAGATGGTTT+TGG	+	chr4_2:58533657-58533676	Msa0610690:CDS	40.0%
	GCAATAAAACTCACCAAAGC+AGG	-	chr4_2:58533771-58533790	None:intergenic	40.0%
	AACTCAAGCCCCTATGAAAA+TGG	+	chr4_2:58533900-58533919	Msa0610690:CDS	40.0%
	AAAGTTGAGATGAGTGACAG+TGG	+	chr4_2:58533933-58533952	Msa0610690:CDS	40.0%
	TGGCGAACTTGACAAATTGA+TGG	+	chr4_2:58533953-58533972	Msa0610690:CDS	40.0%
!	GATATTTTCCCCCTTTGCAA+TGG	+	chr4_2:58534165-58534184	Msa0610690:three_prime_UTR	40.0%
	AGTGAAGAGTGGTAGGGTTA+AGG	-	chr4_2:58532082-58532101	Msa0610680:intron	45.0%
	AGAGAGAGTGAAGAGTGGTA+GGG	-	chr4_2:58532088-58532107	Msa0610680:intron	45.0%
!!	TTGCTGTGGTTGGTGACAAA+GGG	-	chr4_2:58532118-58532137	Msa0610680:intron	45.0%
!!	ATTGCTGTGGTTGGTGACAA+AGG	-	chr4_2:58532119-58532138	Msa0610680:intron	45.0%
	ACCGCAGACAGAGAAAAAGA+TGG	+	chr4_2:58532197-58532216	Msa0610690:exon	45.0%
!	GCCATCTTTTTCTCTGTCTG+CGG	-	chr4_2:58532201-58532220	Msa0610680:intron	45.0%
!	CAGAGAAAAAGATGGCTTGC+AGG	+	chr4_2:58532205-58532224	Msa0610690:exon	45.0%
	AGAGATCCTGAGGTATGATG+CGG	-	chr4_2:58532250-58532269	Msa0610680:intron	45.0%
	TGTGAAAGACAGAGATCCTG+AGG	-	chr4_2:58532260-58532279	Msa0610680:CDS	45.0%
!	GTCCGCCGTATATCAACTTT+TGG	+	chr4_2:58532540-58532559	Msa0610690:intron	45.0%
	CAGATGAGAAGACAGAGACT+CGG	-	chr4_2:58533332-58533351	None:intergenic	45.0%
	GACGGAAAATCTGCAACCAT+TGG	+	chr4_2:58533564-58533583	Msa0610690:CDS	45.0%
	GGCATGATCAGTTATGCCAA+TGG	-	chr4_2:58533583-58533602	None:intergenic	45.0%
	TGAATTGCCAGAAGTGGGAA+CGG	+	chr4_2:58533632-58533651	Msa0610690:CDS	45.0%
!	TGGTGCAGTTGAAAGTGTGA+AGG	+	chr4_2:58533677-58533696	Msa0610690:CDS	45.0%
	GACATCAACTCTCCTGTTTC+AGG	+	chr4_2:58533708-58533727	Msa0610690:CDS	45.0%
	CAAGCCCCTATGAAAATGGA+TGG	+	chr4_2:58533904-58533923	Msa0610690:CDS	45.0%
	TCGGCAAGACTATCAATGAC+TGG	-	chr4_2:58534018-58534037	None:intergenic	45.0%
	GTGGTAGGGTTAAGGAAGTT+AGG	-	chr4_2:58532074-58532093	Msa0610680:intron	45.0%
	AGTGAAGAGTGGTAGGGTTA+AGG	-	chr4_2:58532082-58532101	Msa0610680:intron	45.0%
	AGAGAGAGTGAAGAGTGGTA+GGG	-	chr4_2:58532088-58532107	Msa0610680:intron	45.0%
!!	TTGCTGTGGTTGGTGACAAA+GGG	-	chr4_2:58532118-58532137	Msa0610680:intron	45.0%
!!	ATTGCTGTGGTTGGTGACAA+AGG	-	chr4_2:58532119-58532138	Msa0610680:intron	45.0%
	ACCGCAGACAGAGAAAAAGA+TGG	+	chr4_2:58532197-58532216	Msa0610690:exon	45.0%
!	GCCATCTTTTTCTCTGTCTG+CGG	-	chr4_2:58532201-58532220	Msa0610680:intron	45.0%
!	CAGAGAAAAAGATGGCTTGC+AGG	+	chr4_2:58532205-58532224	Msa0610690:exon	45.0%
	AGAGATCCTGAGGTATGATG+CGG	-	chr4_2:58532250-58532269	Msa0610680:intron	45.0%
	TGTGAAAGACAGAGATCCTG+AGG	-	chr4_2:58532260-58532279	Msa0610680:CDS	45.0%
!	GTCCGCCGTATATCAACTTT+TGG	+	chr4_2:58532540-58532559	Msa0610690:intron	45.0%
	CAGATGAGAAGACAGAGACT+CGG	-	chr4_2:58533332-58533351	None:intergenic	45.0%
	GACGGAAAATCTGCAACCAT+TGG	+	chr4_2:58533564-58533583	Msa0610690:CDS	45.0%
	GGCATGATCAGTTATGCCAA+TGG	-	chr4_2:58533583-58533602	None:intergenic	45.0%
	TGAATTGCCAGAAGTGGGAA+CGG	+	chr4_2:58533632-58533651	Msa0610690:CDS	45.0%
!	TGGTGCAGTTGAAAGTGTGA+AGG	+	chr4_2:58533677-58533696	Msa0610690:CDS	45.0%
	GACATCAACTCTCCTGTTTC+AGG	+	chr4_2:58533708-58533727	Msa0610690:CDS	45.0%
	CAAGCCCCTATGAAAATGGA+TGG	+	chr4_2:58533904-58533923	Msa0610690:CDS	45.0%
	TCGGCAAGACTATCAATGAC+TGG	-	chr4_2:58534018-58534037	None:intergenic	45.0%
	GAGAGAGAGTGAAGAGTGGT+AGG	-	chr4_2:58532089-58532108	Msa0610680:intron	50.0%
	CTGAGAGAGAGAGTGAAGAG+TGG	-	chr4_2:58532093-58532112	Msa0610680:intron	50.0%
!	AGATGGCTTGCAGGCAGTTA+TGG	+	chr4_2:58532214-58532233	Msa0610690:exon	50.0%
!	GATGGCTTGCAGGCAGTTAT+GGG	+	chr4_2:58532215-58532234	Msa0610690:exon	50.0%
	ACGGCAGTGAAACAAGGAGA+TGG	+	chr4_2:58533651-58533670	Msa0610690:CDS	50.0%
	GAGAGAGAGTGAAGAGTGGT+AGG	-	chr4_2:58532089-58532108	Msa0610680:intron	50.0%
	CTGAGAGAGAGAGTGAAGAG+TGG	-	chr4_2:58532093-58532112	Msa0610680:intron	50.0%
!	AGATGGCTTGCAGGCAGTTA+TGG	+	chr4_2:58532214-58532233	Msa0610690:exon	50.0%
!	GATGGCTTGCAGGCAGTTAT+GGG	+	chr4_2:58532215-58532234	Msa0610690:exon	50.0%
	ACGGCAGTGAAACAAGGAGA+TGG	+	chr4_2:58533651-58533670	Msa0610690:CDS	50.0%
	TTCACTGCCGTTCCCACTTC+TGG	-	chr4_2:58533642-58533661	None:intergenic	55.0%
	GTGGGAACGGCAGTGAAACA+AGG	+	chr4_2:58533645-58533664	Msa0610690:CDS	55.0%
	GCTCAGCAACTCTCCTGCTT+TGG	+	chr4_2:58533755-58533774	Msa0610690:CDS	55.0%
!	TGCGCTAGCTTCTGATGCAG+AGG	+	chr4_2:58534139-58534158	Msa0610690:three_prime_UTR	55.0%
!	GCGCTAGCTTCTGATGCAGA+GGG	+	chr4_2:58534140-58534159	Msa0610690:three_prime_UTR	55.0%
	TTCACTGCCGTTCCCACTTC+TGG	-	chr4_2:58533642-58533661	None:intergenic	55.0%
	GTGGGAACGGCAGTGAAACA+AGG	+	chr4_2:58533645-58533664	Msa0610690:CDS	55.0%
	GCTCAGCAACTCTCCTGCTT+TGG	+	chr4_2:58533755-58533774	Msa0610690:CDS	55.0%
!	TGCGCTAGCTTCTGATGCAG+AGG	+	chr4_2:58534139-58534158	Msa0610690:three_prime_UTR	55.0%
!	GCGCTAGCTTCTGATGCAGA+GGG	+	chr4_2:58534140-58534159	Msa0610690:three_prime_UTR	55.0%
	CCTGAGGTATGATGCGGCTC+TGG	-	chr4_2:58532244-58532263	Msa0610680:intron	60.0%
	CCAGAGCCGCATCATACCTC+AGG	+	chr4_2:58532241-58532260	Msa0610690:CDS	60.0%
	CCTGAGGTATGATGCGGCTC+TGG	-	chr4_2:58532244-58532263	Msa0610680:intron	60.0%
	CCAGAGCCGCATCATACCTC+AGG	+	chr4_2:58532241-58532260	Msa0610690:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4_2	gene	58532074	58534327	58532074	ID=Msa0610690;Name=Msa0610690
chr4_2	mRNA	58532074	58534327	58532074	ID=Msa0610690-mRNA-1;Parent=Msa0610690;Name=Msa0610690-mRNA-1;_AED=0.07;_eAED=0.07;_QI=142\|1\|1\|1\|1\|1\|3\|302\|155
chr4_2	exon	58532074	58532294	58532074	ID=Msa0610690-mRNA-1:exon:15361;Parent=Msa0610690-mRNA-1
chr4_2	exon	58533517	58533776	58533517	ID=Msa0610690-mRNA-1:exon:15362;Parent=Msa0610690-mRNA-1
chr4_2	exon	58533897	58534327	58533897	ID=Msa0610690-mRNA-1:exon:15363;Parent=Msa0610690-mRNA-1
chr4_2	five_prime_UTR	58532074	58532215	58532074	ID=Msa0610690-mRNA-1:five_prime_utr;Parent=Msa0610690-mRNA-1
chr4_2	CDS	58532216	58532294	58532216	ID=Msa0610690-mRNA-1:cds;Parent=Msa0610690-mRNA-1
chr4_2	CDS	58533517	58533776	58533517	ID=Msa0610690-mRNA-1:cds;Parent=Msa0610690-mRNA-1
chr4_2	CDS	58533897	58534025	58533897	ID=Msa0610690-mRNA-1:cds;Parent=Msa0610690-mRNA-1
chr4_2	three_prime_UTR	58534026	58534327	58534026	ID=Msa0610690-mRNA-1:three_prime_utr;Parent=Msa0610690-mRNA-1

Gene Sequence

>Msa0610690

ATGGCTTGCAGGCAGTTATGGGCATCCAGAGCCGCATCATACCTCAGGATCTCTGTCTTTCACAGAACCTTCTCTAACGTTCTGAACAATCTGAAGTATGCTGATTCTCATGAATGGGTGAAAGTTGACGGAAAATCTGCAACCATTGGCATAACTGATCATGCCCAAGATCATTTGGGTGATGTTGTGTATGTTGAATTGCCAGAAGTGGGAACGGCAGTGAAACAAGGAGATGGTTTTGGTGCAGTTGAAAGTGTGAAGGCAACTAGTGACATCAACTCTCCTGTTTCAGGAAAAGTGATTGAAGTTAATGAAGAGCTCAGCAACTCTCCTGCTTTGGTCAACTCAAGCCCCTATGAAAATGGATGGATAATTAAAGTTGAGATGAGTGACAGTGGCGAACTTGACAAATTGATGGATTCAGAAAAGTATACCAAGTTCTGTGAAGAAGAAGATTCCAGTCATTGA

Protein sequence

>Msa0610690

MACRQLWASRAASYLRISVFHRTFSNVLNNLKYADSHEWVKVDGKSATIGITDHAQDHLGDVVYVELPEVGTAVKQGDGFGAVESVKATSDINSPVSGKVIEVNEELSNSPALVNSSPYENGWIIKVEMSDSGELDKLMDSEKYTKFCEEEDSSH*