AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0605200

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0605200 MtrunA17_Chr4g0034381 96.863 255 8 0 1 255 1 255 0.0 522
Msa0605200 MtrunA17_Chr4g0020211 30.270 185 117 4 12 194 5 179 7.43e-26 102
Msa0605200 MtrunA17_Chr2g0297031 29.630 189 117 7 19 195 7 191 1.09e-19 85.5
Msa0605200 MtrunA17_Chr8g0378231 33.333 180 97 8 26 194 25 192 2.39e-18 84.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0605200 AT4G34360.1 65.289 242 82 1 11 252 6 245 3.02e-122 348
Msa0605200 AT3G60910.1 28.972 214 140 4 20 231 13 216 1.74e-28 109
Msa0605200 AT3G17365.1 33.505 194 105 8 10 198 2 176 4.58e-25 99.8
Msa0605200 AT3G17365.2 28.700 223 115 9 10 198 2 214 1.26e-18 83.2
Msa0605200 AT2G31740.1 26.316 190 119 5 16 194 19 198 4.97e-15 74.7
Msa0605200 AT3G60910.2 27.439 164 108 3 69 231 1 154 8.55e-15 71.2

Find 86 sgRNAs with CRISPR-Local

Find 186 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTAAATTATAGTCGTTTA+AGG 0.102919 4_2:-50951493 Msa0605200:three_prime_UTR
TCCACTAACTACAATTAATA+AGG 0.217850 4_2:+50951395 None:intergenic
TTTGAAAGTGACAGTTAAAT+AGG 0.236024 4_2:+50951630 None:intergenic
TACAAAAGTGACACTTAAAA+AGG 0.260629 4_2:+50951666 None:intergenic
GCAAAATCGTTTACTTTCTC+AGG 0.281452 4_2:-50953914 Msa0605200:CDS
AGGTTTGTACACGGTTATTA+AGG 0.287387 4_2:-50951473 Msa0605200:three_prime_UTR
ATATTTATCTCAATAACTTT+TGG 0.294771 4_2:-50952825 Msa0605200:CDS
TTGGACATAGTTTCGGGCTT+TGG 0.296551 4_2:+50952897 None:intergenic
ATTCATGCCAGCCACATTTC+AGG 0.301834 4_2:-50952731 Msa0605200:intron
GTTGAATGATGTGACGAAAA+TGG 0.306994 4_2:+50954067 None:intergenic
AATAAATAAAGGTATATTAC+TGG 0.321346 4_2:-50951350 None:intergenic
AAATCCGGTGCATTAAATAT+AGG 0.331855 4_2:+50952705 None:intergenic
CCCTTATTAATTGTAGTTAG+TGG 0.335556 4_2:-50951396 Msa0605200:three_prime_UTR
GGCACTTCAATCTTCTTAAC+AGG 0.339241 4_2:+50951803 None:intergenic
AAGCTCGAGTACAGAGGAAT+TGG 0.341672 4_2:+50954032 None:intergenic
TATTAAGGAAATGATTAGTT+TGG 0.352807 4_2:-50951458 Msa0605200:three_prime_UTR
AATACCAGTAAGAAGGATTA+AGG 0.357541 4_2:+50954227 None:intergenic
CGTACCTTAATCCTTCTTAC+TGG 0.358053 4_2:-50954231 Msa0605200:intron
TGATGAAATTCAATAAATAA+AGG 0.358298 4_2:-50951361 Msa0605200:three_prime_UTR
CATGACCTTGGACATAGTTT+CGG 0.360959 4_2:+50952890 None:intergenic
TCTGTTGAGTGGACAACTTT+TGG 0.374812 4_2:-50952675 Msa0605200:CDS
ATTGAGATAAATATGCCATT+TGG 0.404045 4_2:+50952834 None:intergenic
AGAGGAATTGGGTGTGAGAT+GGG 0.404822 4_2:+50954044 None:intergenic
CCACTAACTACAATTAATAA+GGG 0.405428 4_2:+50951396 None:intergenic
CTCTCCACAATAAATAACTA+AGG 0.415475 4_2:+50951569 None:intergenic
GGTTATCGAGAAAGGAACTA+TGG 0.416741 4_2:-50953087 Msa0605200:intron
ATGACCTTGGACATAGTTTC+GGG 0.418705 4_2:+50952891 None:intergenic
AATCCGGTGCATTAAATATA+GGG 0.420986 4_2:+50952706 None:intergenic
GTTTCGGGCTTTGGATTCCA+TGG 0.425961 4_2:+50952906 None:intergenic
TTTGATTTGGTTATCGAGAA+AGG 0.436453 4_2:-50953095 Msa0605200:CDS
GCTACAGTTGCCGTTGTCGT+CGG 0.450317 4_2:+50954271 None:intergenic
CTACAGTTGCCGTTGTCGTC+GGG 0.455982 4_2:+50954272 None:intergenic
CTTCACTTTCTAACTCCTCA+TGG 0.461373 4_2:+50951765 None:intergenic
CAGAGGAATTGGGTGTGAGA+TGG 0.462348 4_2:+50954043 None:intergenic
CCTAGCGCCGCCGGACAAAA+CGG 0.470816 4_2:+50954323 None:intergenic
TTGAATGATGTGACGAAAAT+GGG 0.471196 4_2:+50954068 None:intergenic
TTCTCTCTTTCAGGGATGAG+AGG 0.477120 4_2:-50954131 Msa0605200:intron
AATTCCTCTGTACTCGAGCT+TGG 0.483015 4_2:-50954030 Msa0605200:CDS
CCACATTGAAAGGTGTTCAC+AGG 0.484252 4_2:-50952866 Msa0605200:CDS
TTGTCCGGCGGCGCTAGGGT+TGG 0.496171 4_2:-50954318 Msa0605200:five_prime_UTR
TTTGAACAGCAACATGAGAA+AGG 0.496949 4_2:+50953941 None:intergenic
AGCTCGAGTACAGAGGAATT+GGG 0.499953 4_2:+50954033 None:intergenic
TTAATGCACCGGATTTCAGC+TGG 0.501862 4_2:-50952698 Msa0605200:CDS
TCTGTACTCGAGCTTGGATG+TGG 0.506204 4_2:-50954024 Msa0605200:CDS
TTACCCTTAGTTATTTATTG+TGG 0.506382 4_2:-50951573 Msa0605200:three_prime_UTR
TCAACAGACCAGCTGAAATC+CGG 0.509055 4_2:+50952690 None:intergenic
CACATTGAAAGGTGTTCACA+GGG 0.510309 4_2:-50952865 Msa0605200:CDS
AAGGTCAATGCAAGTAATGT+TGG 0.513041 4_2:+50953960 None:intergenic
TTTCAATGTGGACATGACCT+TGG 0.513884 4_2:+50952878 None:intergenic
TTTGAAGGATGTATTGTTCG+TGG 0.515742 4_2:-50952940 Msa0605200:intron
CAAAATCGTTTACTTTCTCA+GGG 0.517884 4_2:-50953913 Msa0605200:CDS
CTGCACTCAGTTGGACAAAA+CGG 0.518983 4_2:+50954361 None:intergenic
TCAAAACACTCATCGTCAAA+AGG 0.520279 4_2:+50953113 None:intergenic
TTACTTTCTCAGGGATTCAA+AGG 0.522486 4_2:-50953904 Msa0605200:intron
ACTTCCAACCCTAGCGCCGC+CGG 0.524672 4_2:+50954314 None:intergenic
ATTTCAGCTGGTCTGTTGAG+TGG 0.525474 4_2:-50952686 Msa0605200:CDS
GCGCCCTATATTTAATGCAC+CGG 0.527854 4_2:-50952709 Msa0605200:CDS
AGGTACGCCGATGCAGTTGA+GGG 0.528333 4_2:+50954247 None:intergenic
TTTCGGGCTTTGGATTCCAT+GGG 0.537272 4_2:+50952907 None:intergenic
AAGGTCATGTCCACATTGAA+AGG 0.542953 4_2:-50952876 Msa0605200:CDS
ATTAGTGAATACCAGTAAGA+AGG 0.546958 4_2:+50954220 None:intergenic
TTGCCACGCCTGCACTCAGT+TGG 0.560921 4_2:+50954352 None:intergenic
GATGTATTGTTCGTGGATAG+TGG 0.567892 4_2:-50952933 Msa0605200:CDS
CGAAACACACCCGACGACAA+CGG 0.571642 4_2:-50954281 Msa0605200:CDS
TGTCCAACTGAGTGCAGGCG+TGG 0.577607 4_2:-50954355 Msa0605200:five_prime_UTR
TCTCCACAATAAATAACTAA+GGG 0.579404 4_2:+50951570 None:intergenic
AAGGTACGCCGATGCAGTTG+AGG 0.583684 4_2:+50954246 None:intergenic
AATATAGGGCGCCTGAAATG+TGG 0.601319 4_2:+50952720 None:intergenic
CGACTATAATTTAGAGACGG+AGG 0.604117 4_2:+50951500 None:intergenic
ACTTCAATCTTCTTAACAGG+TGG 0.607989 4_2:+50951806 None:intergenic
AAAGCCCGAAACTATGTCCA+AGG 0.612081 4_2:-50952895 Msa0605200:CDS
GAGGAATTGGGTGTGAGATG+GGG 0.615697 4_2:+50954045 None:intergenic
GACTATAATTTAGAGACGGA+GGG 0.622572 4_2:+50951501 None:intergenic
AAACGACTATAATTTAGAGA+CGG 0.633680 4_2:+50951497 None:intergenic
TCGTGGATAGTGGTGACCCA+TGG 0.650075 4_2:-50952923 Msa0605200:CDS
CTAGGGTTGGAAGTCAACAA+TGG 0.653241 4_2:-50954305 Msa0605200:exon
TGTAGCACCCTCAACTGCAT+CGG 0.661050 4_2:-50954254 Msa0605200:CDS
TGTGATGGCTTGTATAACGA+TGG 0.668784 4_2:-50953988 Msa0605200:CDS
CCTGTGAACACCTTTCAATG+TGG 0.670686 4_2:+50952866 None:intergenic
AGTCGTTTAAGGTTTGTACA+CGG 0.678757 4_2:-50951482 Msa0605200:three_prime_UTR
ACATCCAAGCTCGAGTACAG+AGG 0.684098 4_2:+50954026 None:intergenic
TCATGGAACAGATTAAGATG+TGG 0.692597 4_2:+50951782 None:intergenic
GGAAACTCACAAATGTGTGA+TGG 0.698096 4_2:-50954003 Msa0605200:CDS
ACATCTTAATCTGTTCCATG+AGG 0.704259 4_2:-50951780 Msa0605200:CDS
CAGGGATGAGAGGTTCACGA+AGG 0.711423 4_2:-50954121 Msa0605200:CDS
CGAAGGAAGAGAAATACGAG+TGG 0.736788 4_2:-50954104 Msa0605200:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTTATCTCAATAACTTT+TGG - chr4_2:50952886-50952905 Msa0605200:CDS 15.0%
!! ATTTATATATGAAACTACAT+TGG + chr4_2:50953420-50953439 None:intergenic 15.0%
!! TGATGAAATTCAATAAATAA+AGG - chr4_2:50954350-50954369 Msa0605200:five_prime_UTR 15.0%
!! AAAACAACTTATAGCTTATA+CGG - chr4_2:50952202-50952221 Msa0605200:intron 20.0%
!! CTGAAAATAATATCATAAGT+TGG + chr4_2:50952312-50952331 None:intergenic 20.0%
!!! CAACTTATGATATTATTTTC+AGG - chr4_2:50952310-50952329 Msa0605200:intron 20.0%
!!! TCTTTTAATGATGATTTACT+TGG - chr4_2:50952378-50952397 Msa0605200:intron 20.0%
!!! CTTTTAATGATGATTTACTT+GGG - chr4_2:50952379-50952398 Msa0605200:intron 20.0%
!!! TTTTAATGATGATTTACTTG+GGG - chr4_2:50952380-50952399 Msa0605200:intron 20.0%
!!! GTTTTGGTAAATAAAAGTAA+TGG - chr4_2:50952411-50952430 Msa0605200:intron 20.0%
!!! TAATGGATTTGTTTTGTATA+TGG - chr4_2:50952428-50952447 Msa0605200:intron 20.0%
!! TTTGTATATGGTTTAAACTT+TGG - chr4_2:50952440-50952459 Msa0605200:intron 20.0%
!! ACAATCTAAACTAACAATTA+TGG - chr4_2:50952692-50952711 Msa0605200:CDS 20.0%
!!! TATGTTAACTTTGATTTTGA+AGG - chr4_2:50952756-50952775 Msa0605200:intron 20.0%
!!! TTTTAAGAACATTAGAATCA+AGG + chr4_2:50952961-50952980 None:intergenic 20.0%
!! AAGTTTATAATGTGATATCA+GGG + chr4_2:50953227-50953246 None:intergenic 20.0%
!!! AAGTTGTTTAATTCTAGTTT+TGG - chr4_2:50953743-50953762 Msa0605200:intron 20.0%
!!! TTTAATTCTAGTTTTGGTTA+GGG - chr4_2:50953749-50953768 Msa0605200:intron 20.0%
!!! TATTAAGGAAATGATTAGTT+TGG - chr4_2:50954253-50954272 Msa0605200:CDS 20.0%
! ATAAGTTACATAGTAGTAGA+AGG + chr4_2:50951841-50951860 None:intergenic 25.0%
! GTAATGTTTCGTGAAATTAT+TGG - chr4_2:50951887-50951906 Msa0605200:intron 25.0%
!!! TTTTTCAATTAAGACGATAG+AGG - chr4_2:50951972-50951991 Msa0605200:intron 25.0%
! GTATGAACTTATAAGCTATA+AGG - chr4_2:50952122-50952141 Msa0605200:intron 25.0%
! TTTAATGATGATTTACTTGG+GGG - chr4_2:50952381-50952400 Msa0605200:intron 25.0%
!! AATTTTCCTTTGTCCTTTAA+TGG - chr4_2:50952657-50952676 Msa0605200:CDS 25.0%
! AAAATTCCATTAAAGGACAA+AGG + chr4_2:50952666-50952685 None:intergenic 25.0%
!! TTTGTCCTTTAATGGAATTT+TGG - chr4_2:50952665-50952684 Msa0605200:CDS 25.0%
! ATTGAGATAAATATGCCATT+TGG + chr4_2:50952880-50952899 None:intergenic 25.0%
!!! TTTTGTGTATGTTCTAAAGA+AGG - chr4_2:50953074-50953093 Msa0605200:intron 25.0%
! AAAAAAAAAAAGAGGCTGTA+TGG + chr4_2:50953108-50953127 None:intergenic 25.0%
! AGTTTATAATGTGATATCAG+GGG + chr4_2:50953226-50953245 None:intergenic 25.0%
! CAAGTTTATAATGTGATATC+AGG + chr4_2:50953228-50953247 None:intergenic 25.0%
! CTGATATCACATTATAAACT+TGG - chr4_2:50953226-50953245 Msa0605200:intron 25.0%
! TTCTTAGATTGACATCAAAT+CGG + chr4_2:50953295-50953314 None:intergenic 25.0%
!! CAATCTAAGAACTAATTTGT+TGG - chr4_2:50953304-50953323 Msa0605200:intron 25.0%
!! ATTTCTTTTCACAAAGTTAC+TGG - chr4_2:50953372-50953391 Msa0605200:intron 25.0%
! AAAGTTACTGGATTTATAAG+TGG - chr4_2:50953384-50953403 Msa0605200:intron 25.0%
! CTTTGCAATGATATAGAAAA+TGG - chr4_2:50953530-50953549 Msa0605200:intron 25.0%
! ATAATCAGATAGATTTCATC+AGG - chr4_2:50953574-50953593 Msa0605200:intron 25.0%
! AGAAAAAAACAAAACTGTAC+TGG + chr4_2:50953607-50953626 None:intergenic 25.0%
!!! GTTTAATTCTAGTTTTGGTT+AGG - chr4_2:50953748-50953767 Msa0605200:intron 25.0%
! TACAAAAGTGACACTTAAAA+AGG + chr4_2:50954048-50954067 None:intergenic 25.0%
! TTTGAAAGTGACAGTTAAAT+AGG + chr4_2:50954084-50954103 None:intergenic 25.0%
! TTACCCTTAGTTATTTATTG+TGG - chr4_2:50954138-50954157 Msa0605200:intron 25.0%
! TCTCCACAATAAATAACTAA+GGG + chr4_2:50954144-50954163 None:intergenic 25.0%
! AAACGACTATAATTTAGAGA+CGG + chr4_2:50954217-50954236 None:intergenic 25.0%
! CTCTAAATTATAGTCGTTTA+AGG - chr4_2:50954218-50954237 Msa0605200:intron 25.0%
! CCACTAACTACAATTAATAA+GGG + chr4_2:50954318-50954337 None:intergenic 25.0%
! TCCACTAACTACAATTAATA+AGG + chr4_2:50954319-50954338 None:intergenic 25.0%
AATACCAGTAAGAAGGATTA+AGG + chr4_2:50951487-50951506 None:intergenic 30.0%
ATTAGTGAATACCAGTAAGA+AGG + chr4_2:50951494-50951513 None:intergenic 30.0%
AGTTCAGAAAAATGACTAGA+AGG + chr4_2:50951530-50951549 None:intergenic 30.0%
! ATTGTTTGTTTCTCTCTTTC+AGG - chr4_2:50951571-50951590 Msa0605200:three_prime_UTR 30.0%
! TTGTTTGTTTCTCTCTTTCA+GGG - chr4_2:50951572-50951591 Msa0605200:three_prime_UTR 30.0%
TTGAATGATGTGACGAAAAT+GGG + chr4_2:50951646-50951665 None:intergenic 30.0%
CAAAATCGTTTACTTTCTCA+GGG - chr4_2:50951798-50951817 Msa0605200:CDS 30.0%
CATTATTCTACAAGTCAGTT+TGG + chr4_2:50952282-50952301 None:intergenic 30.0%
!! CAAACTGACTTGTAGAATAA+TGG - chr4_2:50952280-50952299 Msa0605200:intron 30.0%
TCTCATTATCTGATTCTGTT+TGG - chr4_2:50952486-50952505 Msa0605200:intron 30.0%
!! TTTGATTTGGTTATCGAGAA+AGG - chr4_2:50952616-50952635 Msa0605200:intron 30.0%
GAAAGGAACTATGGTAAATT+AGG - chr4_2:50952633-50952652 Msa0605200:intron 30.0%
TCAAGCCAAAATTCCATTAA+AGG + chr4_2:50952673-50952692 None:intergenic 30.0%
AATCCGGTGCATTAAATATA+GGG + chr4_2:50953008-50953027 None:intergenic 30.0%
AAATCCGGTGCATTAAATAT+AGG + chr4_2:50953009-50953028 None:intergenic 30.0%
!!! GTCTGCTTTTTGATATATTG+AGG - chr4_2:50953159-50953178 Msa0605200:intron 30.0%
! TAGATAGATGAACATTTTCC+AGG - chr4_2:50953856-50953875 Msa0605200:intron 30.0%
! AGATAGATGAACATTTTCCA+GGG - chr4_2:50953857-50953876 Msa0605200:intron 30.0%
CTCTCCACAATAAATAACTA+AGG + chr4_2:50954145-50954164 None:intergenic 30.0%
CCCTTATTAATTGTAGTTAG+TGG - chr4_2:50954315-50954334 Msa0605200:five_prime_UTR 30.0%
GTTGAATGATGTGACGAAAA+TGG + chr4_2:50951647-50951666 None:intergenic 35.0%
AAGGTCAATGCAAGTAATGT+TGG + chr4_2:50951754-50951773 None:intergenic 35.0%
TTTGAACAGCAACATGAGAA+AGG + chr4_2:50951773-50951792 None:intergenic 35.0%
GCAAAATCGTTTACTTTCTC+AGG - chr4_2:50951797-50951816 Msa0605200:CDS 35.0%
TTACTTTCTCAGGGATTCAA+AGG - chr4_2:50951807-50951826 Msa0605200:CDS 35.0%
GAAAATGACAGTTACCAAAC+AGG - chr4_2:50952087-50952106 Msa0605200:intron 35.0%
AGGACTTATAGTCTAATGTG+AGG - chr4_2:50952330-50952349 Msa0605200:intron 35.0%
TCAAAACACTCATCGTCAAA+AGG + chr4_2:50952601-50952620 None:intergenic 35.0%
!!! TGACGATGAGTGTTTTGATT+TGG - chr4_2:50952603-50952622 Msa0605200:intron 35.0%
TCTCAAGCTTAAGAACTGTA+GGG + chr4_2:50952719-50952738 None:intergenic 35.0%
TTTGAAGGATGTATTGTTCG+TGG - chr4_2:50952771-50952790 Msa0605200:intron 35.0%
! TATCTCAATAACTTTTGGCC+AGG - chr4_2:50952891-50952910 Msa0605200:CDS 35.0%
AAGAGTTCATTCTTCAAACC+TGG + chr4_2:50952912-50952931 None:intergenic 35.0%
AGAACATTAGAATCAAGGCA+GGG + chr4_2:50952956-50952975 None:intergenic 35.0%
AAGAACATTAGAATCAAGGC+AGG + chr4_2:50952957-50952976 None:intergenic 35.0%
! AATGTGATATCAGGGGATTT+TGG + chr4_2:50953219-50953238 None:intergenic 35.0%
GGCAAAGATTTCTTCAATGA+AGG - chr4_2:50953325-50953344 Msa0605200:intron 35.0%
GTTACTGGATTTATAAGTGG+AGG - chr4_2:50953387-50953406 Msa0605200:intron 35.0%
! TTTCTTCCTTTTCTACCTTC+TGG - chr4_2:50953622-50953641 Msa0605200:intron 35.0%
AAACAACTTGAGCAAAACAC+AGG + chr4_2:50953732-50953751 None:intergenic 35.0%
ACTTCAATCTTCTTAACAGG+TGG + chr4_2:50953908-50953927 None:intergenic 35.0%
TCATGGAACAGATTAAGATG+TGG + chr4_2:50953932-50953951 None:intergenic 35.0%
ACATCTTAATCTGTTCCATG+AGG - chr4_2:50953931-50953950 Msa0605200:CDS 35.0%
GACACTTAAAAAGGAACAGA+GGG + chr4_2:50954039-50954058 None:intergenic 35.0%
TGACACTTAAAAAGGAACAG+AGG + chr4_2:50954040-50954059 None:intergenic 35.0%
GACTATAATTTAGAGACGGA+GGG + chr4_2:50954213-50954232 None:intergenic 35.0%
! AGTCGTTTAAGGTTTGTACA+CGG - chr4_2:50954229-50954248 Msa0605200:intron 35.0%
AGGTTTGTACACGGTTATTA+AGG - chr4_2:50954238-50954257 Msa0605200:CDS 35.0%
! GCAATTATCACCGTTTTGTC+CGG - chr4_2:50951378-50951397 Msa0605200:three_prime_UTR 40.0%
CGTACCTTAATCCTTCTTAC+TGG - chr4_2:50951480-50951499 Msa0605200:three_prime_UTR 40.0%
GGAAACTCACAAATGTGTGA+TGG - chr4_2:50951708-50951727 Msa0605200:exon 40.0%
! TGTGATGGCTTGTATAACGA+TGG - chr4_2:50951723-50951742 Msa0605200:exon 40.0%
ACGACTAAAACAGACCTGTT+TGG + chr4_2:50952104-50952123 None:intergenic 40.0%
AAGTTATCTTGCAGTCTGCT+TGG + chr4_2:50952178-50952197 None:intergenic 40.0%
GATGATTTACTTGGGGGAAT+TGG - chr4_2:50952387-50952406 Msa0605200:intron 40.0%
GGTTATCGAGAAAGGAACTA+TGG - chr4_2:50952624-50952643 Msa0605200:intron 40.0%
CTCTCAAGCTTAAGAACTGT+AGG + chr4_2:50952720-50952739 None:intergenic 40.0%
GATGTATTGTTCGTGGATAG+TGG - chr4_2:50952778-50952797 Msa0605200:intron 40.0%
ATGACCTTGGACATAGTTTC+GGG + chr4_2:50952823-50952842 None:intergenic 40.0%
CATGACCTTGGACATAGTTT+CGG + chr4_2:50952824-50952843 None:intergenic 40.0%
TTTCAATGTGGACATGACCT+TGG + chr4_2:50952836-50952855 None:intergenic 40.0%
AAGGTCATGTCCACATTGAA+AGG - chr4_2:50952835-50952854 Msa0605200:CDS 40.0%
!! CACATTGAAAGGTGTTCACA+GGG - chr4_2:50952846-50952865 Msa0605200:CDS 40.0%
!!! CACAGGGTTTTGAAACCAAA+TGG - chr4_2:50952862-50952881 Msa0605200:CDS 40.0%
! TCTGTTGAGTGGACAACTTT+TGG - chr4_2:50953036-50953055 Msa0605200:intron 40.0%
AAAGAGCCAGAAGGTAGAAA+AGG + chr4_2:50953631-50953650 None:intergenic 40.0%
!!! TTCTACCTTCTGGCTCTTTT+TGG - chr4_2:50953632-50953651 Msa0605200:intron 40.0%
GTAACACAACTCTCCATCAA+AGG + chr4_2:50953707-50953726 None:intergenic 40.0%
!!! TCTAGTTTTGGTTAGGGAAG+AGG - chr4_2:50953755-50953774 Msa0605200:intron 40.0%
!!! CTAGTTTTGGTTAGGGAAGA+GGG - chr4_2:50953756-50953775 Msa0605200:intron 40.0%
!!! TAGTTTTGGTTAGGGAAGAG+GGG - chr4_2:50953757-50953776 Msa0605200:intron 40.0%
GGCACTTCAATCTTCTTAAC+AGG + chr4_2:50953911-50953930 None:intergenic 40.0%
CTTCACTTTCTAACTCCTCA+TGG + chr4_2:50953949-50953968 None:intergenic 40.0%
CGACTATAATTTAGAGACGG+AGG + chr4_2:50954214-50954233 None:intergenic 40.0%
! ATTATCACCGTTTTGTCCGG+CGG - chr4_2:50951381-50951400 Msa0605200:three_prime_UTR 45.0%
CTAGGGTTGGAAGTCAACAA+TGG - chr4_2:50951406-50951425 Msa0605200:three_prime_UTR 45.0%
TTCTCTCTTTCAGGGATGAG+AGG - chr4_2:50951580-50951599 Msa0605200:three_prime_UTR 45.0%
CGAAGGAAGAGAAATACGAG+TGG - chr4_2:50951607-50951626 Msa0605200:three_prime_UTR 45.0%
AGAGGAATTGGGTGTGAGAT+GGG + chr4_2:50951670-50951689 None:intergenic 45.0%
AGCTCGAGTACAGAGGAATT+GGG + chr4_2:50951681-50951700 None:intergenic 45.0%
AAGCTCGAGTACAGAGGAAT+TGG + chr4_2:50951682-50951701 None:intergenic 45.0%
AATTCCTCTGTACTCGAGCT+TGG - chr4_2:50951681-50951700 Msa0605200:three_prime_UTR 45.0%
!! GTAGCGAATGAGGCTTTGTT+TGG + chr4_2:50952260-50952279 None:intergenic 45.0%
!! AGTCAGTTTGGTAGCGAATG+AGG + chr4_2:50952270-50952289 None:intergenic 45.0%
!! ACTTGGGGGAATTGGTGTTT+TGG - chr4_2:50952395-50952414 Msa0605200:intron 45.0%
!! TTTCGGGCTTTGGATTCCAT+GGG + chr4_2:50952807-50952826 None:intergenic 45.0%
TTGGACATAGTTTCGGGCTT+TGG + chr4_2:50952817-50952836 None:intergenic 45.0%
AAAGCCCGAAACTATGTCCA+AGG - chr4_2:50952816-50952835 Msa0605200:intron 45.0%
CCTGTGAACACCTTTCAATG+TGG + chr4_2:50952848-50952867 None:intergenic 45.0%
!! CCACATTGAAAGGTGTTCAC+AGG - chr4_2:50952845-50952864 Msa0605200:CDS 45.0%
ATTCATGCCAGCCACATTTC+AGG - chr4_2:50952980-50952999 Msa0605200:intron 45.0%
! AATATAGGGCGCCTGAAATG+TGG + chr4_2:50952994-50953013 None:intergenic 45.0%
GCGCCCTATATTTAATGCAC+CGG - chr4_2:50953002-50953021 Msa0605200:intron 45.0%
TTAATGCACCGGATTTCAGC+TGG - chr4_2:50953013-50953032 Msa0605200:intron 45.0%
TCAACAGACCAGCTGAAATC+CGG + chr4_2:50953024-50953043 None:intergenic 45.0%
! ATTTCAGCTGGTCTGTTGAG+TGG - chr4_2:50953025-50953044 Msa0605200:intron 45.0%
!! TATTGAGGTGCTGCTACCAA+AGG - chr4_2:50953174-50953193 Msa0605200:intron 45.0%
AACACATACGTTCGAGCCTT+TGG + chr4_2:50953193-50953212 None:intergenic 45.0%
CGGTCACTGTAGTACAAAAC+TGG + chr4_2:50953275-50953294 None:intergenic 45.0%
AAGCTCCAAAAAGAGCCAGA+AGG + chr4_2:50953640-50953659 None:intergenic 45.0%
!!! TTTTTGGAGCTTGCTACACC+TGG - chr4_2:50953648-50953667 Msa0605200:intron 45.0%
AGGGGGATTCATCCAATCTT+TGG - chr4_2:50953672-50953691 Msa0605200:intron 45.0%
ACACAACTCTCCATCAAAGG+CGG + chr4_2:50953704-50953723 None:intergenic 45.0%
CAACTTGAGCAAAACACAGG+TGG + chr4_2:50953729-50953748 None:intergenic 45.0%
GGGTATGCACGAGATTGATA+AGG - chr4_2:50953777-50953796 Msa0605200:intron 45.0%
! TGAACATTTTCCAGGGACAG+AGG - chr4_2:50953864-50953883 Msa0605200:intron 45.0%
!! TTAAAAAAAAAAAAAAAAAG+AGG + chr4_2:50953116-50953135 None:intergenic 5.0%
TGTAGCACCCTCAACTGCAT+CGG - chr4_2:50951457-50951476 Msa0605200:three_prime_UTR 50.0%
GAGGAATTGGGTGTGAGATG+GGG + chr4_2:50951669-50951688 None:intergenic 50.0%
CAGAGGAATTGGGTGTGAGA+TGG + chr4_2:50951671-50951690 None:intergenic 50.0%
ACATCCAAGCTCGAGTACAG+AGG + chr4_2:50951688-50951707 None:intergenic 50.0%
TCTGTACTCGAGCTTGGATG+TGG - chr4_2:50951687-50951706 Msa0605200:three_prime_UTR 50.0%
!! GTTTCGGGCTTTGGATTCCA+TGG + chr4_2:50952808-50952827 None:intergenic 50.0%
ATTGGATGAATCCCCCTTCC+AGG + chr4_2:50953669-50953688 None:intergenic 50.0%
CACAACTCTCCATCAAAGGC+GGG + chr4_2:50953703-50953722 None:intergenic 50.0%
CGAAACACACCCGACGACAA+CGG - chr4_2:50951430-50951449 Msa0605200:three_prime_UTR 55.0%
CTACAGTTGCCGTTGTCGTC+GGG + chr4_2:50951442-50951461 None:intergenic 55.0%
GCTACAGTTGCCGTTGTCGT+CGG + chr4_2:50951443-50951462 None:intergenic 55.0%
AGGTACGCCGATGCAGTTGA+GGG + chr4_2:50951467-50951486 None:intergenic 55.0%
AAGGTACGCCGATGCAGTTG+AGG + chr4_2:50951468-50951487 None:intergenic 55.0%
CAGGGATGAGAGGTTCACGA+AGG - chr4_2:50951590-50951609 Msa0605200:three_prime_UTR 55.0%
! TCGTGGATAGTGGTGACCCA+TGG - chr4_2:50952788-50952807 Msa0605200:intron 55.0%
!! TGGAGCTTGCTACACCTGGA+AGG - chr4_2:50953652-50953671 Msa0605200:intron 55.0%
!! GGAGCTTGCTACACCTGGAA+GGG - chr4_2:50953653-50953672 Msa0605200:intron 55.0%
!! GAGCTTGCTACACCTGGAAG+GGG - chr4_2:50953654-50953673 Msa0605200:intron 55.0%
!! AGCTTGCTACACCTGGAAGG+GGG - chr4_2:50953655-50953674 Msa0605200:intron 55.0%
AGGCGGGTAGAGCCAAAGAT+TGG + chr4_2:50953687-50953706 None:intergenic 55.0%
!! TTGGCTCTACCCGCCTTTGA+TGG - chr4_2:50953691-50953710 Msa0605200:intron 55.0%
CATGTGATGACCTCTGTCCC+TGG + chr4_2:50953877-50953896 None:intergenic 55.0%
TGTCCAACTGAGTGCAGGCG+TGG - chr4_2:50951356-50951375 Msa0605200:three_prime_UTR 60.0%
TTGCCACGCCTGCACTCAGT+TGG + chr4_2:50951362-50951381 None:intergenic 60.0%
! TAGGGCGCCTGAAATGTGGC+TGG + chr4_2:50952990-50953009 None:intergenic 60.0%
CCTAGCGCCGCCGGACAAAA+CGG + chr4_2:50951391-50951410 None:intergenic 65.0%
! CGTTTTGTCCGGCGGCGCTA+GGG - chr4_2:50951389-50951408 Msa0605200:three_prime_UTR 65.0%
ACTTCCAACCCTAGCGCCGC+CGG + chr4_2:50951400-50951419 None:intergenic 65.0%
! CCGTTTTGTCCGGCGGCGCT+AGG - chr4_2:50951388-50951407 Msa0605200:three_prime_UTR 70.0%
TTGTCCGGCGGCGCTAGGGT+TGG - chr4_2:50951393-50951412 Msa0605200:three_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 50951352 50954381 50951352 ID=Msa0605200;Name=Msa0605200
chr4_2 mRNA 50951352 50954381 50951352 ID=Msa0605200-mRNA-1;Parent=Msa0605200;Name=Msa0605200-mRNA-1;_AED=0.10;_eAED=0.10;_QI=73|1|1|1|1|1|6|372|255
chr4_2 exon 50951352 50951855 50951352 ID=Msa0605200-mRNA-1:exon:12093;Parent=Msa0605200-mRNA-1
chr4_2 exon 50952638 50952742 50952638 ID=Msa0605200-mRNA-1:exon:12092;Parent=Msa0605200-mRNA-1
chr4_2 exon 50952821 50952955 50952821 ID=Msa0605200-mRNA-1:exon:12091;Parent=Msa0605200-mRNA-1
chr4_2 exon 50953088 50953170 50953088 ID=Msa0605200-mRNA-1:exon:12090;Parent=Msa0605200-mRNA-1
chr4_2 exon 50953905 50954140 50953905 ID=Msa0605200-mRNA-1:exon:12089;Parent=Msa0605200-mRNA-1
chr4_2 exon 50954232 50954381 50954232 ID=Msa0605200-mRNA-1:exon:12088;Parent=Msa0605200-mRNA-1
chr4_2 five_prime_UTR 50954309 50954381 50954309 ID=Msa0605200-mRNA-1:five_prime_utr;Parent=Msa0605200-mRNA-1
chr4_2 CDS 50954232 50954308 50954232 ID=Msa0605200-mRNA-1:cds;Parent=Msa0605200-mRNA-1
chr4_2 CDS 50953905 50954140 50953905 ID=Msa0605200-mRNA-1:cds;Parent=Msa0605200-mRNA-1
chr4_2 CDS 50953088 50953170 50953088 ID=Msa0605200-mRNA-1:cds;Parent=Msa0605200-mRNA-1
chr4_2 CDS 50952821 50952955 50952821 ID=Msa0605200-mRNA-1:cds;Parent=Msa0605200-mRNA-1
chr4_2 CDS 50952638 50952742 50952638 ID=Msa0605200-mRNA-1:cds;Parent=Msa0605200-mRNA-1
chr4_2 CDS 50951724 50951855 50951724 ID=Msa0605200-mRNA-1:cds;Parent=Msa0605200-mRNA-1
chr4_2 three_prime_UTR 50951352 50951723 50951352 ID=Msa0605200-mRNA-1:three_prime_utr;Parent=Msa0605200-mRNA-1
Gene Sequence

>Msa0605200

ATGGACGAAACACACCCGACGACAACGGCAACTGTAGCACCCTCAACTGCATCGGCGTACCTTAATCCTTCTTACTGGGATGAGAGGTTCACGAAGGAAGAGAAATACGAGTGGTTCAAAGATTATTCCCATTTTCGTCACATCATTCAACCCCATCTCACACCCAATTCCTCTGTACTCGAGCTTGGATGTGGAAACTCACAAATGTGTGATGGCTTGTATAACGATGGAACTACCAACATTACTTGCATTGACCTTTCTCATGTTGCTGTTCAAAACATGCAAAATCGTTTACTTTCTCAGGGATTCAAAGACATAAAAGTGATGCAAGCTGATATGCTAGAGCTACCTTTTGACGATGAGTGTTTTGATTTGGTTATCGAGAAAGGAACTATGGATGTATTGTTCGTGGATAGTGGTGACCCATGGAATCCAAAGCCCGAAACTATGTCCAAGGTCATGTCCACATTGAAAGGTGTTCACAGGGTTTTGAAACCAAATGGCATATTTATCTCAATAACTTTTGGCCAGCCACATTTCAGGCGCCCTATATTTAATGCACCGGATTTCAGCTGGTCTGTTGAGTGGACAACTTTTGGTGAAACTTTTCACTATTTTGTGTATGTTCTAAAGAAGGGACAGAGGTCATCACATGAAGATATACCACCTGTTAAGAAGATTGAAGTGCCACATCTTAATCTGTTCCATGAGGAGTTAGAAAGTGAAGATTTTGCTTTTCGAATCAATGTTGATGAGTTGAACTCTTAG
Protein sequence

>Msa0605200

MDETHPTTTATVAPSTASAYLNPSYWDERFTKEEKYEWFKDYSHFRHIIQPHLTPNSSVLELGCGNSQMCDGLYNDGTTNITCIDLSHVAVQNMQNRLLSQGFKDIKVMQADMLELPFDDECFDLVIEKGTMDVLFVDSGDPWNPKPETMSKVMSTLKGVHRVLKPNGIFISITFGQPHFRRPIFNAPDFSWSVEWTTFGETFHYFVYVLKKGQRSSHEDIPPVKKIEVPHLNLFHEELESEDFAFRINVDELNS*