AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0127180

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0127180 MtrunA17_Chr1g0185641 100.000 114 0 0 1 114 1 114 2.50e-77 223
Msa0127180 MtrunA17_Chr7g0259691 99.130 115 0 1 1 114 1 115 1.95e-75 218
Msa0127180 MtrunA17_Chr8g0383391 28.319 113 76 2 4 113 9 119 4.93e-12 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0127180 AT4G02840.1 94.792 96 5 0 1 96 1 96 1.37e-61 184
Msa0127180 AT3G07590.2 89.796 98 10 0 1 98 1 98 1.77e-60 181
Msa0127180 AT3G07590.1 89.796 98 10 0 1 98 1 98 1.77e-60 181
Msa0127180 AT4G02840.2 85.849 106 5 1 1 96 1 106 2.83e-58 176
Msa0127180 AT3G07590.3 88.060 67 8 0 30 96 4 70 9.80e-39 125
Msa0127180 AT1G20580.1 29.524 105 72 1 4 108 9 111 2.10e-12 59.7

Find 64 sgRNAs with CRISPR-Local

Find 283 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTACTTTGTTGACTTATAA+AGG 0.256946 1_3:-56398710 Msa0127180:three_prime_UTR
GTACCAGCAGTTGGCTTCTT+AGG 0.288686 1_3:+56399063 None:intergenic
ATTGTTCATGGCACCATTAC+AGG 0.313852 1_3:-56402321 Msa0127180:intron
AATGGATCTTATAATCAATT+AGG 0.323511 1_3:-56398621 Msa0127180:three_prime_UTR
TTGGGGCGTGGGCGCGGTCG+AGG 0.328304 1_3:-56398947 Msa0127180:CDS
ATCATCTTAACTAAGGGAAA+TGG 0.333754 1_3:-56398639 Msa0127180:three_prime_UTR
CGTGGGCGCGGTCGAGGGCG+TGG 0.335495 1_3:-56398941 Msa0127180:CDS
CTTGAATCTTGAGACTTTGC+TGG 0.344909 1_3:-56399109 Msa0127180:CDS
TCAGGGAAGCCTTTGGGGCG+TGG 0.359383 1_3:-56398959 Msa0127180:intron
CAGGGAAGCCTTTGGGGCGT+GGG 0.360605 1_3:-56398958 Msa0127180:CDS
GTTGAGACTTGAGTGGTGTT+AGG 0.377675 1_3:+56398684 None:intergenic
AAGCCTTTGGGGCGTGGGCG+CGG 0.383124 1_3:-56398953 Msa0127180:CDS
CGCGGTCGAGGGCGTGGACG+TGG 0.416042 1_3:-56398935 Msa0127180:CDS
ACGAGCTTCATGGCGGCGAT+GGG 0.419657 1_3:+56402502 None:intergenic
GACGAGCTTCATGGCGGCGA+TGG 0.420396 1_3:+56402501 None:intergenic
GTCTCAAGATTCAAGCTGTC+AGG 0.435893 1_3:+56399117 None:intergenic
TTGTGCCATATGGTTGTGTT+GGG 0.447153 1_3:-56398875 Msa0127180:three_prime_UTR
CTGAGATGATCCAGAGTCAC+TGG 0.455517 1_3:+56399174 None:intergenic
TACTAAAACGGTGTCGTACT+CGG 0.455730 1_3:-56402625 Msa0127180:five_prime_UTR
TCGCCGCCATGAAGCTCGTC+AGG 0.455923 1_3:-56402498 Msa0127180:intron
CTGACCCGCTCCTCCCTTCG+TGG 0.458741 1_3:-56402559 Msa0127180:five_prime_UTR
GAGAGACCTGACGAGCTTCA+TGG 0.459293 1_3:+56402492 None:intergenic
CGTGGACGTGGTCGTGGCCG+CGG 0.466756 1_3:-56398923 Msa0127180:CDS
TTCTTGCTGAATAGTTATGC+TGG 0.470656 1_3:-56398814 Msa0127180:three_prime_UTR
TGGGGCGTGGGCGCGGTCGA+GGG 0.476857 1_3:-56398946 Msa0127180:CDS
CGAGGGCGTGGACGTGGTCG+TGG 0.479147 1_3:-56398929 Msa0127180:CDS
TGGTTGTTGAAGTCGAAAAC+CGG 0.484878 1_3:-56402539 Msa0127180:five_prime_UTR
AAGAACGGTACAATTGTTCA+TGG 0.494710 1_3:-56402333 Msa0127180:CDS
TTTGTGCCATATGGTTGTGT+TGG 0.496936 1_3:-56398876 Msa0127180:three_prime_UTR
GGAATTTATGAGTGGTCTTG+TGG 0.505964 1_3:-56398851 Msa0127180:three_prime_UTR
CTCTGGATCATCTCAGTGTG+AGG 0.510476 1_3:-56399167 Msa0127180:CDS
CGACCGCGCCCACGCCCCAA+AGG 0.511469 1_3:+56398950 None:intergenic
GTTCTTGAGCTCGATCGATA+CGG 0.513270 1_3:+56402350 None:intergenic
GGGTTAGTACGAACCTGTAA+TGG 0.514920 1_3:+56402308 None:intergenic
TGAGACTTTGCTGGTTGAAG+AGG 0.514939 1_3:-56399100 Msa0127180:CDS
GGCTTCTTAGGCTTGACCCT+AGG 0.515024 1_3:+56399075 None:intergenic
GCTGGTTGAAGAGGCACCTA+GGG 0.521169 1_3:-56399091 Msa0127180:CDS
TGCCATATGGTTGTGTTGGG+AGG 0.521995 1_3:-56398872 Msa0127180:three_prime_UTR
TTCCTCCCAACACAACCATA+TGG 0.528839 1_3:+56398870 None:intergenic
ACATAAGGGCAATACTAAAA+CGG 0.529212 1_3:-56402637 None:intergenic
AACAGTTAAACTAACACTGA+AGG 0.539350 1_3:-56399205 Msa0127180:CDS
AACAACCACGAAGGGAGGAG+CGG 0.542438 1_3:+56402554 None:intergenic
ACAACCACGAAGGGAGGAGC+GGG 0.547610 1_3:+56402555 None:intergenic
AAGCCTAAGAAGCCAACTGC+TGG 0.548844 1_3:-56399066 Msa0127180:intron
CTCAGTTAAAGAACTCAGAA+AGG 0.555067 1_3:+56398734 None:intergenic
CGAGCTTCATGGCGGCGATG+GGG 0.570180 1_3:+56402503 None:intergenic
GCTTCATGGCGGCGATGGGG+CGG 0.570668 1_3:+56402506 None:intergenic
ACAGTTAAACTAACACTGAA+GGG 0.577307 1_3:-56399204 Msa0127180:CDS
TCGACTTCAACAACCACGAA+GGG 0.578273 1_3:+56402546 None:intergenic
TCTGGATCATCTCAGTGTGA+GGG 0.591054 1_3:-56399166 Msa0127180:CDS
TGCTGGTTGAAGAGGCACCT+AGG 0.593545 1_3:-56399092 Msa0127180:CDS
TAATGTAAAGTACCAGCAGT+TGG 0.601300 1_3:+56399054 None:intergenic
TGTTGGGAGGAATTTATGAG+TGG 0.610163 1_3:-56398859 Msa0127180:three_prime_UTR
ATGGGGCGGAAGATGAGCGC+CGG 0.618208 1_3:+56402520 None:intergenic
GTATCGATCGAGCTCAAGAA+CGG 0.620082 1_3:-56402348 Msa0127180:CDS
CAAGATTCAAGCTGTCAGGA+AGG 0.623754 1_3:+56399121 None:intergenic
CTGGATCATCTCAGTGTGAG+GGG 0.636050 1_3:-56399165 Msa0127180:CDS
CAGTTAAACTAACACTGAAG+GGG 0.636399 1_3:-56399203 Msa0127180:CDS
ACTTCAACAACCACGAAGGG+AGG 0.645006 1_3:+56402549 None:intergenic
TTCGACTTCAACAACCACGA+AGG 0.651469 1_3:+56402545 None:intergenic
TGAGATGATCCAGAGTCACT+GGG 0.651786 1_3:+56399175 None:intergenic
GACTTTAGTTGAGACTTGAG+TGG 0.672289 1_3:+56398677 None:intergenic
AATATGCAATTCAACGACCG+CGG 0.741788 1_3:+56398906 None:intergenic
AGACCTGACGAGCTTCATGG+CGG 0.747768 1_3:+56402495 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATATTTTAAAATTCAAGAA+GGG + chr1_3:56399111-56399130 None:intergenic 10.0%
!!! TAATATTTTAAAATTCAAGA+AGG + chr1_3:56399112-56399131 None:intergenic 10.0%
!! ATAAATTACAAAGTATAAAA+TGG + chr1_3:56399229-56399248 None:intergenic 10.0%
!!! TTTTATACTTTGTAATTTAT+AGG - chr1_3:56399229-56399248 Msa0127180:CDS 10.0%
!!! TATTTTTTAAAACCATTTAT+CGG - chr1_3:56400301-56400320 Msa0127180:intron 10.0%
!!! TTTATTTATTTTAAAGTTCA+AGG - chr1_3:56400650-56400669 Msa0127180:intron 10.0%
!!! ATGAAAAAAATTAATTTGAA+CGG - chr1_3:56400750-56400769 Msa0127180:intron 10.0%
!! ATGAATACAATCTTATTATA+TGG + chr1_3:56400008-56400027 None:intergenic 15.0%
!!! GATAAATGGTTTTAAAAAAT+AGG + chr1_3:56400302-56400321 None:intergenic 15.0%
!!! TTATATAGTTTTACTTTGTT+AGG - chr1_3:56401530-56401549 Msa0127180:intron 15.0%
!! AAAGACTTAGATATATTAAA+TGG + chr1_3:56401859-56401878 None:intergenic 15.0%
!!! ATATCTAAGTCTTTTAATTT+TGG - chr1_3:56401865-56401884 Msa0127180:intron 15.0%
!!! TATCTAAGTCTTTTAATTTT+GGG - chr1_3:56401866-56401885 Msa0127180:intron 15.0%
!!! GAAAGTTTTTCTTTGAAAAT+CGG - chr1_3:56398790-56398809 Msa0127180:three_prime_UTR 20.0%
!!! TTTTTTGTTGTTGTTGAAAT+AGG - chr1_3:56399137-56399156 Msa0127180:CDS 20.0%
!! TTGATTGTAGTACAAAATAT+AGG + chr1_3:56399257-56399276 None:intergenic 20.0%
!! ATATCATATCATAATAGACT+TGG - chr1_3:56399503-56399522 Msa0127180:intron 20.0%
!! TCAAAAATTGTATTGAAAGA+GGG + chr1_3:56399771-56399790 None:intergenic 20.0%
!!! TCTTTCAATACAATTTTTGA+GGG - chr1_3:56399771-56399790 Msa0127180:intron 20.0%
!!! TATGTGTTTTCTTAATTTGT+TGG - chr1_3:56400572-56400591 Msa0127180:intron 20.0%
!! GAATATATAATACTTCTCTT+AGG + chr1_3:56401191-56401210 None:intergenic 20.0%
!!! AACGTTTTAATTTCATTTTC+AGG - chr1_3:56402217-56402236 Msa0127180:intron 20.0%
!!! ACGTTTTAATTTCATTTTCA+GGG - chr1_3:56402218-56402237 Msa0127180:intron 20.0%
!!! TGTTTTTATCATCTTAACTA+AGG - chr1_3:56402548-56402567 Msa0127180:five_prime_UTR 20.0%
!!! GTTTTTATCATCTTAACTAA+GGG - chr1_3:56402549-56402568 Msa0127180:five_prime_UTR 20.0%
!! AATGGATCTTATAATCAATT+AGG - chr1_3:56402573-56402592 Msa0127180:five_prime_UTR 20.0%
!!! TGCACAAGTGTTTTATTTTT+AGG + chr1_3:56398599-56398618 None:intergenic 25.0%
!!! AAACAAGAAGTTGATAGAAA+CGG + chr1_3:56398744-56398763 None:intergenic 25.0%
! GATGTATAATAACAGTAATG+AGG + chr1_3:56398921-56398940 None:intergenic 25.0%
!! GTTATTGTTATTATTCCTCA+TGG - chr1_3:56399018-56399037 Msa0127180:intron 25.0%
!!! ATTACACTGTCATTTTTCTA+AGG - chr1_3:56399350-56399369 Msa0127180:intron 25.0%
!!! TTACACTGTCATTTTTCTAA+GGG - chr1_3:56399351-56399370 Msa0127180:intron 25.0%
! CAGAACAACATCATATTATA+TGG + chr1_3:56399469-56399488 None:intergenic 25.0%
! GTATTATAACAGCATAGATT+AGG - chr1_3:56399726-56399745 Msa0127180:intron 25.0%
! CTCAAAAATTGTATTGAAAG+AGG + chr1_3:56399772-56399791 None:intergenic 25.0%
!! CTCTTTCAATACAATTTTTG+AGG - chr1_3:56399770-56399789 Msa0127180:intron 25.0%
!!! CTTTCAATACAATTTTTGAG+GGG - chr1_3:56399772-56399791 Msa0127180:intron 25.0%
! TGATATACATCCATTGAATT+AGG + chr1_3:56399962-56399981 None:intergenic 25.0%
! TATCTTGCCAAATTGTAATA+GGG + chr1_3:56400200-56400219 None:intergenic 25.0%
! TAGAATGTTAGAAGAAGAAA+GGG + chr1_3:56400382-56400401 None:intergenic 25.0%
! ATAGAATGTTAGAAGAAGAA+AGG + chr1_3:56400383-56400402 None:intergenic 25.0%
!!! TGTTTTCTTAATTTGTTGGT+TGG - chr1_3:56400576-56400595 Msa0127180:intron 25.0%
! AAATGTATGAATGATTTGAC+TGG + chr1_3:56401320-56401339 None:intergenic 25.0%
! AAAACTAAAATCACGAAAAC+GGG + chr1_3:56401343-56401362 None:intergenic 25.0%
! GAAAACTAAAATCACGAAAA+CGG + chr1_3:56401344-56401363 None:intergenic 25.0%
! CTAAAAAAATGGCTTAGATT+AGG + chr1_3:56401391-56401410 None:intergenic 25.0%
!! TTGGTGTGTTTCTAAAAAAA+TGG + chr1_3:56401402-56401421 None:intergenic 25.0%
! AACAAAGTAAAACTAAATCC+AGG + chr1_3:56401489-56401508 None:intergenic 25.0%
!!! GGATTTAGTTTTACTTTGTT+AGG - chr1_3:56401489-56401508 Msa0127180:intron 25.0%
!! AACGCAATTACTTTCTTTTA+TGG - chr1_3:56401670-56401689 Msa0127180:intron 25.0%
! TGCTTGACTAGTATTATATT+TGG + chr1_3:56401758-56401777 None:intergenic 25.0%
! AGTTAATAGTAAAACCATAC+TGG - chr1_3:56401796-56401815 Msa0127180:intron 25.0%
! AAGCACTTGTTCAAAAATAA+TGG + chr1_3:56402420-56402439 None:intergenic 25.0%
!!! ACTACTTTGTTGACTTATAA+AGG - chr1_3:56402484-56402503 Msa0127180:intron 25.0%
!! TTTTCTTTGAAAATCGGAAC+AGG - chr1_3:56398796-56398815 Msa0127180:three_prime_UTR 30.0%
CAGTAATGAGGAAGAAAAAA+GGG + chr1_3:56398909-56398928 None:intergenic 30.0%
ACAGTAATGAGGAAGAAAAA+AGG + chr1_3:56398910-56398929 None:intergenic 30.0%
!!! AAAGGGTTTTTAGGGTTTAA+CGG + chr1_3:56398957-56398976 None:intergenic 30.0%
! AATGTAGTGATTTTCTTCTC+CGG - chr1_3:56399074-56399093 Msa0127180:CDS 30.0%
AAGAAGGGTTTATTAGATTC+CGG + chr1_3:56399096-56399115 None:intergenic 30.0%
! TAGGTTGCTTAGTTTGAATA+TGG - chr1_3:56399156-56399175 Msa0127180:CDS 30.0%
!!! TGTATTTGTGAGTTGGTTTT+TGG + chr1_3:56399548-56399567 None:intergenic 30.0%
CATAATTTCCATTTCTCTCT+TGG + chr1_3:56399918-56399937 None:intergenic 30.0%
GTTTGTTCCCTATTACAATT+TGG - chr1_3:56400190-56400209 Msa0127180:intron 30.0%
GTATCTTGCCAAATTGTAAT+AGG + chr1_3:56400201-56400220 None:intergenic 30.0%
!!! GTTTTAAAAAATAGGCGATG+TGG + chr1_3:56400294-56400313 None:intergenic 30.0%
! ATCAAAATTTTAGACACCTG+TGG + chr1_3:56400329-56400348 None:intergenic 30.0%
! CAACTGTATCTCTTTTACAT+TGG + chr1_3:56400538-56400557 None:intergenic 30.0%
CAATGTAAAAGAGATACAGT+TGG - chr1_3:56400536-56400555 Msa0127180:intron 30.0%
TAAGAAAACACATATCGTCA+TGG + chr1_3:56400566-56400585 None:intergenic 30.0%
ACTTTCTTAGACCATGTAAT+TGG + chr1_3:56400915-56400934 None:intergenic 30.0%
AATTACATGGTCTAAGAAAG+TGG - chr1_3:56400914-56400933 Msa0127180:intron 30.0%
TAAAATGCATAGGCTGATAT+GGG + chr1_3:56401168-56401187 None:intergenic 30.0%
TTCTCTTAGGTAAAATGCAT+AGG + chr1_3:56401178-56401197 None:intergenic 30.0%
AATGGCTTAGATTAGGTAAA+AGG + chr1_3:56401384-56401403 None:intergenic 30.0%
! AATTTTCTATTCAACACACC+TGG - chr1_3:56401468-56401487 Msa0127180:intron 30.0%
! GCAATTACTTTCTTTTATGG+AGG - chr1_3:56401673-56401692 Msa0127180:intron 30.0%
! GGTTTTACTATTAACTGTCA+AGG + chr1_3:56401792-56401811 None:intergenic 30.0%
AACAGTTAAACTAACACTGA+AGG - chr1_3:56401989-56402008 Msa0127180:intron 30.0%
ACAGTTAAACTAACACTGAA+GGG - chr1_3:56401990-56402009 Msa0127180:intron 30.0%
!! ACTTGCTGCAATTTTTATTC+AGG - chr1_3:56402167-56402186 Msa0127180:intron 30.0%
! TTCAGAATTTTTGTGCCATA+TGG - chr1_3:56402309-56402328 Msa0127180:intron 30.0%
ATCATCTTAACTAAGGGAAA+TGG - chr1_3:56402555-56402574 Msa0127180:five_prime_UTR 30.0%
GCTGCATGATAAAAATTGTA+GGG - chr1_3:56402610-56402629 Msa0127180:five_prime_UTR 30.0%
AAGAACGGTACAATTGTTCA+TGG - chr1_3:56398861-56398880 Msa0127180:three_prime_UTR 35.0%
! AACTGATAGTTTAAGTGCTG+AGG - chr1_3:56399195-56399214 Msa0127180:CDS 35.0%
!! ACTGATAGTTTAAGTGCTGA+GGG - chr1_3:56399196-56399215 Msa0127180:CDS 35.0%
!!! TTTGTGAGTTGGTTTTTGGA+GGG + chr1_3:56399544-56399563 None:intergenic 35.0%
!!! ATTTGTGAGTTGGTTTTTGG+AGG + chr1_3:56399545-56399564 None:intergenic 35.0%
CTTAGAGTGTATTTGTGAGT+TGG + chr1_3:56399555-56399574 None:intergenic 35.0%
CAACTCACAAATACACTCTA+AGG - chr1_3:56399553-56399572 Msa0127180:intron 35.0%
ATAAGCACGCTCAAAAAACA+TGG + chr1_3:56399655-56399674 None:intergenic 35.0%
AGGAAAGGAAAATGGAAGAA+AGG + chr1_3:56399851-56399870 None:intergenic 35.0%
!! GAAAGCTTTGGATAAAAGGA+AGG + chr1_3:56399874-56399893 None:intergenic 35.0%
!! AGAGGAAAGCTTTGGATAAA+AGG + chr1_3:56399878-56399897 None:intergenic 35.0%
TTTCCCTCTTCCTAATTCAA+TGG - chr1_3:56399949-56399968 Msa0127180:intron 35.0%
CATCCATTGAATTAGGAAGA+GGG + chr1_3:56399955-56399974 None:intergenic 35.0%
ACATCCATTGAATTAGGAAG+AGG + chr1_3:56399956-56399975 None:intergenic 35.0%
TAATAGGGAACAAACACCAT+TGG + chr1_3:56400185-56400204 None:intergenic 35.0%
! TGTTGAGTGAGTGTTTTACT+AGG + chr1_3:56400265-56400284 None:intergenic 35.0%
AAGAAAGGGCAAACAACAAA+AGG + chr1_3:56400368-56400387 None:intergenic 35.0%
! TTTGTGGTAGTGAACGAAAA+TGG - chr1_3:56400432-56400451 Msa0127180:intron 35.0%
! TTGTGGTAGTGAACGAAAAT+GGG - chr1_3:56400433-56400452 Msa0127180:intron 35.0%
TTAACAAGAGAGAGCTTGAA+TGG - chr1_3:56400478-56400497 Msa0127180:intron 35.0%
TAACAAGAGAGAGCTTGAAT+GGG - chr1_3:56400479-56400498 Msa0127180:intron 35.0%
! ATGGATTGCTCTATGAAACA+TGG + chr1_3:56400782-56400801 None:intergenic 35.0%
ATAGAGCAATCCATTATGCA+GGG - chr1_3:56400788-56400807 Msa0127180:intron 35.0%
TTGCAGTTTCAGAGAGATAA+TGG - chr1_3:56400862-56400881 Msa0127180:intron 35.0%
TGCAGTTTCAGAGAGATAAT+GGG - chr1_3:56400863-56400882 Msa0127180:intron 35.0%
TCGCTCTACATATTAAACTC+TGG - chr1_3:56401000-56401019 Msa0127180:intron 35.0%
CCAATTGTCCAAAAACAACA+AGG + chr1_3:56401094-56401113 None:intergenic 35.0%
!!! CCTTGTTGTTTTTGGACAAT+TGG - chr1_3:56401091-56401110 Msa0127180:intron 35.0%
! TTTTTGGACAATTGGACTGA+AGG - chr1_3:56401099-56401118 Msa0127180:intron 35.0%
AAAATGCATAGGCTGATATG+GGG + chr1_3:56401167-56401186 None:intergenic 35.0%
GTAAAATGCATAGGCTGATA+TGG + chr1_3:56401169-56401188 None:intergenic 35.0%
TGCAATTCGTGAGATTGTTT+CGG + chr1_3:56401213-56401232 None:intergenic 35.0%
ATCTCACGAATTGCAAGAAA+AGG - chr1_3:56401218-56401237 Msa0127180:intron 35.0%
ACGAATTGCAAGAAAAGGAT+AGG - chr1_3:56401223-56401242 Msa0127180:intron 35.0%
TGAGGTTTAATAAGAAGCTG+GGG + chr1_3:56401431-56401450 None:intergenic 35.0%
GTGAGGTTTAATAAGAAGCT+GGG + chr1_3:56401432-56401451 None:intergenic 35.0%
TGTGAGGTTTAATAAGAAGC+TGG + chr1_3:56401433-56401452 None:intergenic 35.0%
TGCTAAAGTTTGTGATGTGT+TGG - chr1_3:56401605-56401624 Msa0127180:intron 35.0%
GCTAAAGTTTGTGATGTGTT+GGG - chr1_3:56401606-56401625 Msa0127180:intron 35.0%
TGACTAACAATTCATGAAGC+TGG + chr1_3:56401643-56401662 None:intergenic 35.0%
ATAATACTAGTCAAGCACCA+AGG - chr1_3:56401761-56401780 Msa0127180:intron 35.0%
TAACTGTCAAGGAAAATCCT+TGG + chr1_3:56401781-56401800 None:intergenic 35.0%
AATAGTAAAACCATACTGGC+TGG - chr1_3:56401800-56401819 Msa0127180:intron 35.0%
CAGTTAAACTAACACTGAAG+GGG - chr1_3:56401991-56402010 Msa0127180:intron 35.0%
! TAATGTAAAGTACCAGCAGT+TGG + chr1_3:56402143-56402162 None:intergenic 35.0%
! GCTGCAATTTTTATTCAGGT+TGG - chr1_3:56402171-56402190 Msa0127180:intron 35.0%
GTTGAAAACCAAAAACCAGA+TGG + chr1_3:56402200-56402219 None:intergenic 35.0%
TTCTTGCTGAATAGTTATGC+TGG - chr1_3:56402380-56402399 Msa0127180:intron 35.0%
CTCAGTTAAAGAACTCAGAA+AGG + chr1_3:56402463-56402482 None:intergenic 35.0%
CGCTGCATGATAAAAATTGT+AGG - chr1_3:56402609-56402628 Msa0127180:five_prime_UTR 35.0%
TGGTTGTTGAAGTCGAAAAC+CGG - chr1_3:56398655-56398674 Msa0127180:three_prime_UTR 40.0%
ATTGTTCATGGCACCATTAC+AGG - chr1_3:56398873-56398892 Msa0127180:three_prime_UTR 40.0%
! TTGAGCGCAAAGGGTTTTTA+GGG + chr1_3:56398965-56398984 None:intergenic 40.0%
! TTTGAGCGCAAAGGGTTTTT+AGG + chr1_3:56398966-56398985 None:intergenic 40.0%
AAGCAATCTTTGAGCGCAAA+GGG + chr1_3:56398974-56398993 None:intergenic 40.0%
TAAGCAATCTTTGAGCGCAA+AGG + chr1_3:56398975-56398994 None:intergenic 40.0%
!! TGTGTTTGCGAGTTGGATTT+TGG - chr1_3:56399374-56399393 Msa0127180:intron 40.0%
!!! TTGTGAGTTGGTTTTTGGAG+GGG + chr1_3:56399543-56399562 None:intergenic 40.0%
AGTTTGGGGAGAGAAGATTA+GGG - chr1_3:56399611-56399630 Msa0127180:intron 40.0%
AACAAACCCCCTATTCAAGT+TGG - chr1_3:56399814-56399833 Msa0127180:intron 40.0%
! ACAAACCCCCTATTCAAGTT+GGG - chr1_3:56399815-56399834 Msa0127180:intron 40.0%
AAGGAAGGAGGAAAGGAAAA+TGG + chr1_3:56399859-56399878 None:intergenic 40.0%
TGGATAAAAGGAAGGAGGAA+AGG + chr1_3:56399866-56399885 None:intergenic 40.0%
!! AGCTTTGGATAAAAGGAAGG+AGG + chr1_3:56399871-56399890 None:intergenic 40.0%
TCTTGGACACTGTTTGTAAG+GGG + chr1_3:56399901-56399920 None:intergenic 40.0%
CTCTTGGACACTGTTTGTAA+GGG + chr1_3:56399902-56399921 None:intergenic 40.0%
TCTCTTGGACACTGTTTGTA+AGG + chr1_3:56399903-56399922 None:intergenic 40.0%
AACAGTGTCCAAGAGAGAAA+TGG - chr1_3:56399907-56399926 Msa0127180:intron 40.0%
!! AAACCATTTATCGGTTCCAC+AGG - chr1_3:56400310-56400329 Msa0127180:intron 40.0%
ATTCTATGAAGCAAAGCTCG+AGG - chr1_3:56400396-56400415 Msa0127180:intron 40.0%
AGCTTGAATGGGAAAAGAAG+AGG - chr1_3:56400490-56400509 Msa0127180:intron 40.0%
TGAATGGGAAAAGAAGAGGA+TGG - chr1_3:56400494-56400513 Msa0127180:intron 40.0%
CATAGAGCAATCCATTATGC+AGG - chr1_3:56400787-56400806 Msa0127180:intron 40.0%
TAGAGCAATCCATTATGCAG+GGG - chr1_3:56400789-56400808 Msa0127180:intron 40.0%
AATCCATTATGCAGGGGAAA+GGG - chr1_3:56400795-56400814 Msa0127180:intron 40.0%
!! TTCAGAGAGATAATGGGATG+AGG - chr1_3:56400869-56400888 Msa0127180:intron 40.0%
!! TCAGAGAGATAATGGGATGA+GGG - chr1_3:56400870-56400889 Msa0127180:intron 40.0%
TGAACCAGTTGCCAATTACA+TGG - chr1_3:56400901-56400920 Msa0127180:intron 40.0%
TAGACCATGTAATTGGCAAC+TGG + chr1_3:56400908-56400927 None:intergenic 40.0%
ACATGGTCTAAGAAAGTGGT+TGG - chr1_3:56400918-56400937 Msa0127180:intron 40.0%
! TCTGGAGTTTAAGCGTTTCT+TGG - chr1_3:56401018-56401037 Msa0127180:intron 40.0%
CTGGAGTTTAAGCGTTTCTT+GGG - chr1_3:56401019-56401038 Msa0127180:intron 40.0%
!!! GAATCCAGCCTTGTTGTTTT+TGG - chr1_3:56401083-56401102 Msa0127180:intron 40.0%
TTGTCCAAAAACAACAAGGC+TGG + chr1_3:56401090-56401109 None:intergenic 40.0%
AGCGTGTAGTTATGCTTTGA+TGG - chr1_3:56401263-56401282 Msa0127180:intron 40.0%
TCAAAAGCTTAAGCTGTGTG+AGG + chr1_3:56401449-56401468 None:intergenic 40.0%
! CTATAGTTGTGTTGTTCCTG+AGG - chr1_3:56401912-56401931 Msa0127180:intron 40.0%
!! TTGACTTGTTGCTATGCTAC+AGG - chr1_3:56401936-56401955 Msa0127180:intron 40.0%
CTTGAATCTTGAGACTTTGC+TGG - chr1_3:56402085-56402104 Msa0127180:intron 40.0%
!! TTCATTTTCAGGGAAGCCTT+TGG - chr1_3:56402228-56402247 Msa0127180:intron 40.0%
!! TCATTTTCAGGGAAGCCTTT+GGG - chr1_3:56402229-56402248 Msa0127180:intron 40.0%
AATATGCAATTCAACGACCG+CGG + chr1_3:56402291-56402310 None:intergenic 40.0%
TTTGTGCCATATGGTTGTGT+TGG - chr1_3:56402318-56402337 Msa0127180:intron 40.0%
TTGTGCCATATGGTTGTGTT+GGG - chr1_3:56402319-56402338 Msa0127180:intron 40.0%
TGTTGGGAGGAATTTATGAG+TGG - chr1_3:56402335-56402354 Msa0127180:CDS 40.0%
! GGAATTTATGAGTGGTCTTG+TGG - chr1_3:56402343-56402362 Msa0127180:CDS 40.0%
GACTTTAGTTGAGACTTGAG+TGG + chr1_3:56402520-56402539 None:intergenic 40.0%
TCGACTTCAACAACCACGAA+GGG + chr1_3:56398651-56398670 None:intergenic 45.0%
TTCGACTTCAACAACCACGA+AGG + chr1_3:56398652-56398671 None:intergenic 45.0%
GTTCTTGAGCTCGATCGATA+CGG + chr1_3:56398847-56398866 None:intergenic 45.0%
! GTATCGATCGAGCTCAAGAA+CGG - chr1_3:56398846-56398865 Msa0127180:three_prime_UTR 45.0%
! GGGTTAGTACGAACCTGTAA+TGG + chr1_3:56398889-56398908 None:intergenic 45.0%
ACATTCACTACTCTGCCATG+AGG + chr1_3:56399036-56399055 None:intergenic 45.0%
GCTATTCGTTGTTGCAGAAC+AGG + chr1_3:56399328-56399347 None:intergenic 45.0%
!! GTTTTTGGAGGGGATGACTT+TGG + chr1_3:56399533-56399552 None:intergenic 45.0%
GAGTTTGGGGAGAGAAGATT+AGG - chr1_3:56399610-56399629 Msa0127180:intron 45.0%
GTTTGGGGAGAGAAGATTAG+GGG - chr1_3:56399612-56399631 Msa0127180:intron 45.0%
!! CAAACCCCCTATTCAAGTTG+GGG - chr1_3:56399816-56399835 Msa0127180:intron 45.0%
TTGCCACTCACTCTAAGTTC+AGG - chr1_3:56400148-56400167 Msa0127180:intron 45.0%
AAGCCTGAACTTAGAGTGAG+TGG + chr1_3:56400154-56400173 None:intergenic 45.0%
ACTCACTCTAAGTTCAGGCT+TGG - chr1_3:56400153-56400172 Msa0127180:intron 45.0%
TCAGGCTTGGAATAGTCCAA+TGG - chr1_3:56400166-56400185 Msa0127180:intron 45.0%
ACACCTGTGGAACCGATAAA+TGG + chr1_3:56400316-56400335 None:intergenic 45.0%
! AGAAGAGGATGGTAGTGAGA+TGG - chr1_3:56400505-56400524 Msa0127180:intron 45.0%
CAATCCATTATGCAGGGGAA+AGG - chr1_3:56400794-56400813 Msa0127180:intron 45.0%
!! CAGAGAGATAATGGGATGAG+GGG - chr1_3:56400871-56400890 Msa0127180:intron 45.0%
!! GATAATGGGATGAGGGGAAA+AGG - chr1_3:56400877-56400896 Msa0127180:intron 45.0%
CTGATATGGGGATCAACAAC+TGG + chr1_3:56401155-56401174 None:intergenic 45.0%
TAAGAAGCTGGGGATGAGAT+TGG + chr1_3:56401421-56401440 None:intergenic 45.0%
ATAGCAACAAGTCAAGCCTC+AGG + chr1_3:56401931-56401950 None:intergenic 45.0%
TGAGATGATCCAGAGTCACT+GGG + chr1_3:56402022-56402041 None:intergenic 45.0%
TCTGGATCATCTCAGTGTGA+GGG - chr1_3:56402028-56402047 Msa0127180:intron 45.0%
CAAGATTCAAGCTGTCAGGA+AGG + chr1_3:56402076-56402095 None:intergenic 45.0%
GTCTCAAGATTCAAGCTGTC+AGG + chr1_3:56402080-56402099 None:intergenic 45.0%
! TGAGACTTTGCTGGTTGAAG+AGG - chr1_3:56402094-56402113 Msa0127180:intron 45.0%
!! CATTTTCAGGGAAGCCTTTG+GGG - chr1_3:56402230-56402249 Msa0127180:intron 45.0%
TTCCTCCCAACACAACCATA+TGG + chr1_3:56402327-56402346 None:intergenic 45.0%
GTTGAGACTTGAGTGGTGTT+AGG + chr1_3:56402513-56402532 None:intergenic 45.0%
ACTTCAACAACCACGAAGGG+AGG + chr1_3:56398648-56398667 None:intergenic 50.0%
! CTAAGGGTGTGTTTGCGAGT+TGG - chr1_3:56399367-56399386 Msa0127180:intron 50.0%
GCTCTGTTTGCGAGTTCAGA+GGG - chr1_3:56399575-56399594 Msa0127180:intron 50.0%
CTCTGTTTGCGAGTTCAGAG+GGG - chr1_3:56399576-56399595 Msa0127180:intron 50.0%
CCAACCCCAACTTGAATAGG+GGG + chr1_3:56399823-56399842 None:intergenic 50.0%
CCCAACCCCAACTTGAATAG+GGG + chr1_3:56399824-56399843 None:intergenic 50.0%
!! CCCCTATTCAAGTTGGGGTT+GGG - chr1_3:56399821-56399840 Msa0127180:intron 50.0%
CCCCAACCCCAACTTGAATA+GGG + chr1_3:56399825-56399844 None:intergenic 50.0%
!! CCCTATTCAAGTTGGGGTTG+GGG - chr1_3:56399822-56399841 Msa0127180:intron 50.0%
ACCCCAACCCCAACTTGAAT+AGG + chr1_3:56399826-56399845 None:intergenic 50.0%
GTAAGGGGAGAGGAAAGCTT+TGG + chr1_3:56399886-56399905 None:intergenic 50.0%
GACACTGTTTGTAAGGGGAG+AGG + chr1_3:56399896-56399915 None:intergenic 50.0%
CTACCACAAATCGTGAGTCG+CGG + chr1_3:56400422-56400441 None:intergenic 50.0%
GTTTGTGATGTGTTGGGCTC+AGG - chr1_3:56401612-56401631 Msa0127180:intron 50.0%
CTGAGATGATCCAGAGTCAC+TGG + chr1_3:56402023-56402042 None:intergenic 50.0%
CTCTGGATCATCTCAGTGTG+AGG - chr1_3:56402027-56402046 Msa0127180:intron 50.0%
CTGGATCATCTCAGTGTGAG+GGG - chr1_3:56402029-56402048 Msa0127180:intron 50.0%
AAGCCTAAGAAGCCAACTGC+TGG - chr1_3:56402128-56402147 Msa0127180:intron 50.0%
! GTACCAGCAGTTGGCTTCTT+AGG + chr1_3:56402134-56402153 None:intergenic 50.0%
!! TATTCAGGTTGGCTGCCATC+TGG - chr1_3:56402182-56402201 Msa0127180:intron 50.0%
!!! GTTGGCTGCCATCTGGTTTT+TGG - chr1_3:56402189-56402208 Msa0127180:intron 50.0%
TGCCATATGGTTGTGTTGGG+AGG - chr1_3:56402322-56402341 Msa0127180:CDS 50.0%
AACAACCACGAAGGGAGGAG+CGG + chr1_3:56398643-56398662 None:intergenic 55.0%
AGACCTGACGAGCTTCATGG+CGG + chr1_3:56398702-56398721 None:intergenic 55.0%
GAGAGACCTGACGAGCTTCA+TGG + chr1_3:56398705-56398724 None:intergenic 55.0%
GGCTCTGTTTGCGAGTTCAG+AGG - chr1_3:56399574-56399593 Msa0127180:intron 55.0%
TGTTTGCGAGTTCAGAGGGG+AGG - chr1_3:56399579-56399598 Msa0127180:intron 55.0%
GTTTGCGAGTTCAGAGGGGA+GGG - chr1_3:56399580-56399599 Msa0127180:intron 55.0%
TTTGCGAGTTCAGAGGGGAG+GGG - chr1_3:56399581-56399600 Msa0127180:intron 55.0%
!! CCCCCTATTCAAGTTGGGGT+TGG - chr1_3:56399820-56399839 Msa0127180:intron 55.0%
CCACCCTTTCCCCTGCATAA+TGG + chr1_3:56400801-56400820 None:intergenic 55.0%
CCATTATGCAGGGGAAAGGG+TGG - chr1_3:56400798-56400817 Msa0127180:intron 55.0%
TTATGCAGGGGAAAGGGTGG+TGG - chr1_3:56400801-56400820 Msa0127180:intron 55.0%
TATGCAGGGGAAAGGGTGGT+GGG - chr1_3:56400802-56400821 Msa0127180:intron 55.0%
TGGGTGAGGTTGAGGGATAG+TGG - chr1_3:56400821-56400840 Msa0127180:intron 55.0%
! GTGAGGTTGAGGGATAGTGG+TGG - chr1_3:56400824-56400843 Msa0127180:intron 55.0%
!! GTTGAGGGATAGTGGTGGAC+TGG - chr1_3:56400829-56400848 Msa0127180:intron 55.0%
!! GGATAGTGGTGGACTGGTGA+CGG - chr1_3:56400835-56400854 Msa0127180:intron 55.0%
GCAACATCTGCCAGCCAGTA+TGG + chr1_3:56401813-56401832 None:intergenic 55.0%
GGGGAAAAACCCAGTGACTC+TGG - chr1_3:56402010-56402029 Msa0127180:intron 55.0%
!! TGCTGGTTGAAGAGGCACCT+AGG - chr1_3:56402102-56402121 Msa0127180:intron 55.0%
!! GCTGGTTGAAGAGGCACCTA+GGG - chr1_3:56402103-56402122 Msa0127180:intron 55.0%
GGCTTCTTAGGCTTGACCCT+AGG + chr1_3:56402122-56402141 None:intergenic 55.0%
ACAACCACGAAGGGAGGAGC+GGG + chr1_3:56398642-56398661 None:intergenic 60.0%
ACGAGCTTCATGGCGGCGAT+GGG + chr1_3:56398695-56398714 None:intergenic 60.0%
GAGTTCAGAGGGGAGGGGAA+GGG - chr1_3:56399586-56399605 Msa0127180:intron 60.0%
GGGAGGGGAAGGGAGAGTTT+GGG - chr1_3:56399596-56399615 Msa0127180:intron 60.0%
GGAGGGGAAGGGAGAGTTTG+GGG - chr1_3:56399597-56399616 Msa0127180:intron 60.0%
AGGCCGCGACTCACGATTTG+TGG - chr1_3:56400416-56400435 Msa0127180:intron 60.0%
!! AAGGGTGGTGGGTGAGGTTG+AGG - chr1_3:56400813-56400832 Msa0127180:intron 60.0%
!! AGGGTGGTGGGTGAGGTTGA+GGG - chr1_3:56400814-56400833 Msa0127180:intron 60.0%
!! TAGTGGTGGACTGGTGACGG+TGG - chr1_3:56400838-56400857 Msa0127180:intron 60.0%
ATGGGGCGGAAGATGAGCGC+CGG + chr1_3:56398677-56398696 None:intergenic 65.0%
CGAGCTTCATGGCGGCGATG+GGG + chr1_3:56398694-56398713 None:intergenic 65.0%
GACGAGCTTCATGGCGGCGA+TGG + chr1_3:56398696-56398715 None:intergenic 65.0%
TCGCCGCCATGAAGCTCGTC+AGG - chr1_3:56398696-56398715 Msa0127180:three_prime_UTR 65.0%
CGAGTTCAGAGGGGAGGGGA+AGG - chr1_3:56399585-56399604 Msa0127180:intron 65.0%
GGGGAGGGGAAGGGAGAGTT+TGG - chr1_3:56399595-56399614 Msa0127180:intron 65.0%
!! AGGGGAAAGGGTGGTGGGTG+AGG - chr1_3:56400807-56400826 Msa0127180:intron 65.0%
!! TCAGGGAAGCCTTTGGGGCG+TGG - chr1_3:56402235-56402254 Msa0127180:intron 65.0%
!! CAGGGAAGCCTTTGGGGCGT+GGG - chr1_3:56402236-56402255 Msa0127180:intron 65.0%
CTGACCCGCTCCTCCCTTCG+TGG - chr1_3:56398635-56398654 Msa0127180:three_prime_UTR 70.0%
GCTTCATGGCGGCGATGGGG+CGG + chr1_3:56398691-56398710 None:intergenic 70.0%
!! AAGCCTTTGGGGCGTGGGCG+CGG - chr1_3:56402241-56402260 Msa0127180:intron 70.0%
! CGAGGGCGTGGACGTGGTCG+TGG - chr1_3:56402265-56402284 Msa0127180:intron 75.0%
CGTGGACGTGGTCGTGGCCG+CGG - chr1_3:56402271-56402290 Msa0127180:intron 75.0%
! CGACCGCGCCCACGCCCCAA+AGG + chr1_3:56402247-56402266 None:intergenic 80.0%
! TTGGGGCGTGGGCGCGGTCG+AGG - chr1_3:56402247-56402266 Msa0127180:intron 80.0%
! TGGGGCGTGGGCGCGGTCGA+GGG - chr1_3:56402248-56402267 Msa0127180:intron 80.0%
!! CGCGGTCGAGGGCGTGGACG+TGG - chr1_3:56402259-56402278 Msa0127180:intron 80.0%
! CGTGGGCGCGGTCGAGGGCG+TGG - chr1_3:56402253-56402272 Msa0127180:intron 85.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 56398571 56402645 56398571 ID=Msa0127180;Name=Msa0127180
chr1_3 mRNA 56398571 56402645 56398571 ID=Msa0127180-mRNA-1;Parent=Msa0127180;Name=Msa0127180-mRNA-1;_AED=0.01;_eAED=0.01;_QI=133|1|1|1|1|1|4|345|114
chr1_3 exon 56398571 56398977 56398571 ID=Msa0127180-mRNA-1:exon:17815;Parent=Msa0127180-mRNA-1
chr1_3 exon 56399067 56399258 56399067 ID=Msa0127180-mRNA-1:exon:17814;Parent=Msa0127180-mRNA-1
chr1_3 exon 56402322 56402398 56402322 ID=Msa0127180-mRNA-1:exon:17813;Parent=Msa0127180-mRNA-1
chr1_3 exon 56402499 56402645 56402499 ID=Msa0127180-mRNA-1:exon:17812;Parent=Msa0127180-mRNA-1
chr1_3 five_prime_UTR 56402513 56402645 56402513 ID=Msa0127180-mRNA-1:five_prime_utr;Parent=Msa0127180-mRNA-1
chr1_3 CDS 56402499 56402512 56402499 ID=Msa0127180-mRNA-1:cds;Parent=Msa0127180-mRNA-1
chr1_3 CDS 56402322 56402398 56402322 ID=Msa0127180-mRNA-1:cds;Parent=Msa0127180-mRNA-1
chr1_3 CDS 56399067 56399258 56399067 ID=Msa0127180-mRNA-1:cds;Parent=Msa0127180-mRNA-1
chr1_3 CDS 56398916 56398977 56398916 ID=Msa0127180-mRNA-1:cds;Parent=Msa0127180-mRNA-1
chr1_3 three_prime_UTR 56398571 56398915 56398571 ID=Msa0127180-mRNA-1:three_prime_utr;Parent=Msa0127180-mRNA-1
Gene Sequence

>Msa0127180

ATGAAGCTCGTCAGGTTTCTGATGAAATTGAACAACGAAACCGTATCGATCGAGCTCAAGAACGGTACAATTGTTCATGGCACCATTACAGGTGTTGATATCAGCATGAACACACATTTGAAAACAGTTAAACTAACACTGAAGGGGAAAAACCCAGTGACTCTGGATCATCTCAGTGTGAGGGGTAACAACATTCGTTATTATATCCTTCCTGACAGCTTGAATCTTGAGACTTTGCTGGTTGAAGAGGCACCTAGGGTCAAGCCTAAGAAGCCAACTGCTGGGAAGCCTTTGGGGCGTGGGCGCGGTCGAGGGCGTGGACGTGGTCGTGGCCGCGGTCGTTGA
Protein sequence

>Msa0127180

MKLVRFLMKLNNETVSIELKNGTIVHGTITGVDISMNTHLKTVKLTLKGKNPVTLDHLSVRGNNIRYYILPDSLNLETLLVEEAPRVKPKKPTAGKPLGRGRGRGRGRGRGRGR*