AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0605140

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0605140 MtrunA17_Chr4g0034381 96.863 255 8 0 1 255 1 255 0.0 522
Msa0605140 MtrunA17_Chr4g0020211 30.270 185 117 4 12 194 5 179 7.43e-26 102
Msa0605140 MtrunA17_Chr2g0297031 29.630 189 117 7 19 195 7 191 1.09e-19 85.5
Msa0605140 MtrunA17_Chr8g0378231 33.333 180 97 8 26 194 25 192 2.39e-18 84.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0605140 AT4G34360.1 65.289 242 82 1 11 252 6 245 3.02e-122 348
Msa0605140 AT3G60910.1 28.972 214 140 4 20 231 13 216 1.74e-28 109
Msa0605140 AT3G17365.1 33.505 194 105 8 10 198 2 176 4.58e-25 99.8
Msa0605140 AT3G17365.2 28.700 223 115 9 10 198 2 214 1.26e-18 83.2
Msa0605140 AT2G31740.1 26.316 190 119 5 16 194 19 198 4.97e-15 74.7
Msa0605140 AT3G60910.2 27.439 164 108 3 69 231 1 154 8.55e-15 71.2

Find 86 sgRNAs with CRISPR-Local

Find 186 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTAAATTATAGTCGTTTA+AGG 0.102919 4_2:-50897943 Msa0605140:three_prime_UTR
TCCACTAACTACAATTAATA+AGG 0.217850 4_2:+50897845 None:intergenic
TTTGAAAGTGACAGTTAAAT+AGG 0.236024 4_2:+50898080 None:intergenic
TACAAAAGTGACACTTAAAA+AGG 0.260629 4_2:+50898116 None:intergenic
GCAAAATCGTTTACTTTCTC+AGG 0.281452 4_2:-50900363 Msa0605140:CDS
AGGTTTGTACACGGTTATTA+AGG 0.287387 4_2:-50897923 Msa0605140:three_prime_UTR
ATATTTATCTCAATAACTTT+TGG 0.294771 4_2:-50899272 Msa0605140:CDS
TTGGACATAGTTTCGGGCTT+TGG 0.296551 4_2:+50899344 None:intergenic
ATTCATGCCAGCCACATTTC+AGG 0.301834 4_2:-50899178 Msa0605140:intron
GTTGAATGATGTGACGAAAA+TGG 0.306994 4_2:+50900516 None:intergenic
AATAAATAAAGGTATATTAC+TGG 0.321346 4_2:-50897800 None:intergenic
AAATCCGGTGCATTAAATAT+AGG 0.331855 4_2:+50899152 None:intergenic
CCCTTATTAATTGTAGTTAG+TGG 0.335556 4_2:-50897846 Msa0605140:three_prime_UTR
GGCACTTCAATCTTCTTAAC+AGG 0.339241 4_2:+50898253 None:intergenic
AAGCTCGAGTACAGAGGAAT+TGG 0.341672 4_2:+50900481 None:intergenic
TATTAAGGAAATGATTAGTT+TGG 0.352807 4_2:-50897908 Msa0605140:three_prime_UTR
AATACCAGTAAGAAGGATTA+AGG 0.357541 4_2:+50900677 None:intergenic
CGTACCTTAATCCTTCTTAC+TGG 0.358053 4_2:-50900681 Msa0605140:intron
TGATGAAATTCAATAAATAA+AGG 0.358298 4_2:-50897811 Msa0605140:three_prime_UTR
CATGACCTTGGACATAGTTT+CGG 0.360959 4_2:+50899337 None:intergenic
TCTGTTGAGTGGACAACTTT+TGG 0.374812 4_2:-50899122 Msa0605140:CDS
ATTGAGATAAATATGCCATT+TGG 0.404045 4_2:+50899281 None:intergenic
AGAGGAATTGGGTGTGAGAT+GGG 0.404822 4_2:+50900493 None:intergenic
CCACTAACTACAATTAATAA+GGG 0.405428 4_2:+50897846 None:intergenic
CTCTCCACAATAAATAACTA+AGG 0.415475 4_2:+50898019 None:intergenic
GGTTATCGAGAAAGGAACTA+TGG 0.416741 4_2:-50899534 Msa0605140:intron
ATGACCTTGGACATAGTTTC+GGG 0.418705 4_2:+50899338 None:intergenic
AATCCGGTGCATTAAATATA+GGG 0.420986 4_2:+50899153 None:intergenic
GTTTCGGGCTTTGGATTCCA+TGG 0.425961 4_2:+50899353 None:intergenic
TTTGATTTGGTTATCGAGAA+AGG 0.436453 4_2:-50899542 Msa0605140:CDS
GCTACAGTTGCCGTTGTCGT+CGG 0.450317 4_2:+50900721 None:intergenic
CTACAGTTGCCGTTGTCGTC+GGG 0.455982 4_2:+50900722 None:intergenic
CTTCACTTTCTAACTCCTCA+TGG 0.461373 4_2:+50898215 None:intergenic
CAGAGGAATTGGGTGTGAGA+TGG 0.462348 4_2:+50900492 None:intergenic
CCTAGCGCCGCCGGACAAAA+CGG 0.470816 4_2:+50900773 None:intergenic
TTGAATGATGTGACGAAAAT+GGG 0.471196 4_2:+50900517 None:intergenic
TTCTCTCTTTCAGGGATGAG+AGG 0.477120 4_2:-50900580 Msa0605140:intron
AATTCCTCTGTACTCGAGCT+TGG 0.483015 4_2:-50900479 Msa0605140:CDS
CCACATTGAAAGGTGTTCAC+AGG 0.484252 4_2:-50899313 Msa0605140:CDS
TTGTCCGGCGGCGCTAGGGT+TGG 0.496171 4_2:-50900768 Msa0605140:five_prime_UTR
TTTGAACAGCAACATGAGAA+AGG 0.496949 4_2:+50900390 None:intergenic
AGCTCGAGTACAGAGGAATT+GGG 0.499953 4_2:+50900482 None:intergenic
TTAATGCACCGGATTTCAGC+TGG 0.501862 4_2:-50899145 Msa0605140:CDS
TCTGTACTCGAGCTTGGATG+TGG 0.506204 4_2:-50900473 Msa0605140:CDS
TTACCCTTAGTTATTTATTG+TGG 0.506382 4_2:-50898023 Msa0605140:three_prime_UTR
TCAACAGACCAGCTGAAATC+CGG 0.509055 4_2:+50899137 None:intergenic
CACATTGAAAGGTGTTCACA+GGG 0.510309 4_2:-50899312 Msa0605140:CDS
AAGGTCAATGCAAGTAATGT+TGG 0.513041 4_2:+50900409 None:intergenic
TTTCAATGTGGACATGACCT+TGG 0.513884 4_2:+50899325 None:intergenic
TTTGAAGGATGTATTGTTCG+TGG 0.515742 4_2:-50899387 Msa0605140:intron
CAAAATCGTTTACTTTCTCA+GGG 0.517884 4_2:-50900362 Msa0605140:CDS
CTGCACTCAGTTGGACAAAA+CGG 0.518983 4_2:+50900811 None:intergenic
TCAAAACACTCATCGTCAAA+AGG 0.520279 4_2:+50899560 None:intergenic
TTACTTTCTCAGGGATTCAA+AGG 0.522486 4_2:-50900353 Msa0605140:intron
ACTTCCAACCCTAGCGCCGC+CGG 0.524672 4_2:+50900764 None:intergenic
ATTTCAGCTGGTCTGTTGAG+TGG 0.525474 4_2:-50899133 Msa0605140:CDS
GCGCCCTATATTTAATGCAC+CGG 0.527854 4_2:-50899156 Msa0605140:CDS
AGGTACGCCGATGCAGTTGA+GGG 0.528333 4_2:+50900697 None:intergenic
TTTCGGGCTTTGGATTCCAT+GGG 0.537272 4_2:+50899354 None:intergenic
AAGGTCATGTCCACATTGAA+AGG 0.542953 4_2:-50899323 Msa0605140:CDS
ATTAGTGAATACCAGTAAGA+AGG 0.546958 4_2:+50900670 None:intergenic
TTGCCACGCCTGCACTCAGT+TGG 0.560921 4_2:+50900802 None:intergenic
GATGTATTGTTCGTGGATAG+TGG 0.567892 4_2:-50899380 Msa0605140:CDS
CGAAACACACCCGACGACAA+CGG 0.571642 4_2:-50900731 Msa0605140:CDS
TGTCCAACTGAGTGCAGGCG+TGG 0.577607 4_2:-50900805 Msa0605140:five_prime_UTR
TCTCCACAATAAATAACTAA+GGG 0.579404 4_2:+50898020 None:intergenic
AAGGTACGCCGATGCAGTTG+AGG 0.583684 4_2:+50900696 None:intergenic
AATATAGGGCGCCTGAAATG+TGG 0.601319 4_2:+50899167 None:intergenic
CGACTATAATTTAGAGACGG+AGG 0.604117 4_2:+50897950 None:intergenic
ACTTCAATCTTCTTAACAGG+TGG 0.607989 4_2:+50898256 None:intergenic
AAAGCCCGAAACTATGTCCA+AGG 0.612081 4_2:-50899342 Msa0605140:CDS
GAGGAATTGGGTGTGAGATG+GGG 0.615697 4_2:+50900494 None:intergenic
GACTATAATTTAGAGACGGA+GGG 0.622572 4_2:+50897951 None:intergenic
AAACGACTATAATTTAGAGA+CGG 0.633680 4_2:+50897947 None:intergenic
TCGTGGATAGTGGTGACCCA+TGG 0.650075 4_2:-50899370 Msa0605140:CDS
CTAGGGTTGGAAGTCAACAA+TGG 0.653241 4_2:-50900755 Msa0605140:exon
TGTAGCACCCTCAACTGCAT+CGG 0.661050 4_2:-50900704 Msa0605140:CDS
TGTGATGGCTTGTATAACGA+TGG 0.668784 4_2:-50900437 Msa0605140:CDS
CCTGTGAACACCTTTCAATG+TGG 0.670686 4_2:+50899313 None:intergenic
AGTCGTTTAAGGTTTGTACA+CGG 0.678757 4_2:-50897932 Msa0605140:three_prime_UTR
ACATCCAAGCTCGAGTACAG+AGG 0.684098 4_2:+50900475 None:intergenic
TCATGGAACAGATTAAGATG+TGG 0.692597 4_2:+50898232 None:intergenic
GGAAACTCACAAATGTGTGA+TGG 0.698096 4_2:-50900452 Msa0605140:CDS
ACATCTTAATCTGTTCCATG+AGG 0.704259 4_2:-50898230 Msa0605140:CDS
CAGGGATGAGAGGTTCACGA+AGG 0.711423 4_2:-50900570 Msa0605140:CDS
CGAAGGAAGAGAAATACGAG+TGG 0.736788 4_2:-50900553 Msa0605140:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTTATCTCAATAACTTT+TGG - chr4_2:50899339-50899358 Msa0605140:CDS 15.0%
!! ATTTATATATGAAACTACAT+TGG + chr4_2:50899873-50899892 None:intergenic 15.0%
!! TGATGAAATTCAATAAATAA+AGG - chr4_2:50900800-50900819 Msa0605140:five_prime_UTR 15.0%
!! AAAACAACTTATAGCTTATA+CGG - chr4_2:50898655-50898674 Msa0605140:intron 20.0%
!! CTGAAAATAATATCATAAGT+TGG + chr4_2:50898765-50898784 None:intergenic 20.0%
!!! CAACTTATGATATTATTTTC+AGG - chr4_2:50898763-50898782 Msa0605140:intron 20.0%
!!! TCTTTTAATGATGATTTACT+TGG - chr4_2:50898831-50898850 Msa0605140:intron 20.0%
!!! CTTTTAATGATGATTTACTT+GGG - chr4_2:50898832-50898851 Msa0605140:intron 20.0%
!!! TTTTAATGATGATTTACTTG+GGG - chr4_2:50898833-50898852 Msa0605140:intron 20.0%
!!! GTTTTGGTAAATAAAAGTAA+TGG - chr4_2:50898864-50898883 Msa0605140:intron 20.0%
!!! TAATGGATTTGTTTTGTATA+TGG - chr4_2:50898881-50898900 Msa0605140:intron 20.0%
!! TTTGTATATGGTTTAAACTT+TGG - chr4_2:50898893-50898912 Msa0605140:intron 20.0%
!! ACAATCTAAACTAACAATTA+TGG - chr4_2:50899145-50899164 Msa0605140:CDS 20.0%
!!! TATGTTAACTTTGATTTTGA+AGG - chr4_2:50899209-50899228 Msa0605140:intron 20.0%
!!! TTTTAAGAACATTAGAATCA+AGG + chr4_2:50899414-50899433 None:intergenic 20.0%
!! AAGTTTATAATGTGATATCA+GGG + chr4_2:50899680-50899699 None:intergenic 20.0%
!!! AAGTTGTTTAATTCTAGTTT+TGG - chr4_2:50900193-50900212 Msa0605140:intron 20.0%
!!! TTTAATTCTAGTTTTGGTTA+GGG - chr4_2:50900199-50900218 Msa0605140:intron 20.0%
!!! TATTAAGGAAATGATTAGTT+TGG - chr4_2:50900703-50900722 Msa0605140:CDS 20.0%
! ATAAGTTACATAGTAGTAGA+AGG + chr4_2:50898292-50898311 None:intergenic 25.0%
! GTAATGTTTCGTGAAATTAT+TGG - chr4_2:50898338-50898357 Msa0605140:intron 25.0%
!!! TTTTTCAATTAAGACGATAG+AGG - chr4_2:50898423-50898442 Msa0605140:intron 25.0%
! GTATGAACTTATAAGCTATA+AGG - chr4_2:50898575-50898594 Msa0605140:intron 25.0%
! TTTAATGATGATTTACTTGG+GGG - chr4_2:50898834-50898853 Msa0605140:intron 25.0%
!! AATTTTCCTTTGTCCTTTAA+TGG - chr4_2:50899110-50899129 Msa0605140:CDS 25.0%
! AAAATTCCATTAAAGGACAA+AGG + chr4_2:50899119-50899138 None:intergenic 25.0%
!! TTTGTCCTTTAATGGAATTT+TGG - chr4_2:50899118-50899137 Msa0605140:CDS 25.0%
! ATTGAGATAAATATGCCATT+TGG + chr4_2:50899333-50899352 None:intergenic 25.0%
!!! TTTTGTGTATGTTCTAAAGA+AGG - chr4_2:50899527-50899546 Msa0605140:intron 25.0%
! AAAAAAAAAAAGAGGCTGTA+TGG + chr4_2:50899561-50899580 None:intergenic 25.0%
! AGTTTATAATGTGATATCAG+GGG + chr4_2:50899679-50899698 None:intergenic 25.0%
! CAAGTTTATAATGTGATATC+AGG + chr4_2:50899681-50899700 None:intergenic 25.0%
! CTGATATCACATTATAAACT+TGG - chr4_2:50899679-50899698 Msa0605140:intron 25.0%
! TTCTTAGATTGACATCAAAT+CGG + chr4_2:50899748-50899767 None:intergenic 25.0%
!! CAATCTAAGAACTAATTTGT+TGG - chr4_2:50899757-50899776 Msa0605140:intron 25.0%
!! ATTTCTTTTCACAAAGTTAC+TGG - chr4_2:50899825-50899844 Msa0605140:intron 25.0%
! AAAGTTACTGGATTTATAAG+TGG - chr4_2:50899837-50899856 Msa0605140:intron 25.0%
! CTTTGCAATGATATAGAAAA+TGG - chr4_2:50899983-50900002 Msa0605140:intron 25.0%
! ATAATCAGATAGATTTCATC+AGG - chr4_2:50900024-50900043 Msa0605140:intron 25.0%
! AGAAAAAAACAAAACTGTAC+TGG + chr4_2:50900057-50900076 None:intergenic 25.0%
!!! GTTTAATTCTAGTTTTGGTT+AGG - chr4_2:50900198-50900217 Msa0605140:intron 25.0%
! TACAAAAGTGACACTTAAAA+AGG + chr4_2:50900498-50900517 None:intergenic 25.0%
! TTTGAAAGTGACAGTTAAAT+AGG + chr4_2:50900534-50900553 None:intergenic 25.0%
! TTACCCTTAGTTATTTATTG+TGG - chr4_2:50900588-50900607 Msa0605140:intron 25.0%
! TCTCCACAATAAATAACTAA+GGG + chr4_2:50900594-50900613 None:intergenic 25.0%
! AAACGACTATAATTTAGAGA+CGG + chr4_2:50900667-50900686 None:intergenic 25.0%
! CTCTAAATTATAGTCGTTTA+AGG - chr4_2:50900668-50900687 Msa0605140:intron 25.0%
! CCACTAACTACAATTAATAA+GGG + chr4_2:50900768-50900787 None:intergenic 25.0%
! TCCACTAACTACAATTAATA+AGG + chr4_2:50900769-50900788 None:intergenic 25.0%
AATACCAGTAAGAAGGATTA+AGG + chr4_2:50897937-50897956 None:intergenic 30.0%
ATTAGTGAATACCAGTAAGA+AGG + chr4_2:50897944-50897963 None:intergenic 30.0%
AGTTCAGAAAAATGACTAGA+AGG + chr4_2:50897980-50897999 None:intergenic 30.0%
!!! TTGTTTTGTTTCTCTCTTTC+AGG - chr4_2:50898022-50898041 Msa0605140:three_prime_UTR 30.0%
!!! TGTTTTGTTTCTCTCTTTCA+GGG - chr4_2:50898023-50898042 Msa0605140:three_prime_UTR 30.0%
TTGAATGATGTGACGAAAAT+GGG + chr4_2:50898097-50898116 None:intergenic 30.0%
CAAAATCGTTTACTTTCTCA+GGG - chr4_2:50898249-50898268 Msa0605140:CDS 30.0%
TCTCATTATCTGATTCTGTT+TGG - chr4_2:50898939-50898958 Msa0605140:intron 30.0%
!! TTTGATTTGGTTATCGAGAA+AGG - chr4_2:50899069-50899088 Msa0605140:intron 30.0%
GAAAGGAACTATGGTAAATT+AGG - chr4_2:50899086-50899105 Msa0605140:CDS 30.0%
TCAAGCCAAAATTCCATTAA+AGG + chr4_2:50899126-50899145 None:intergenic 30.0%
AATCCGGTGCATTAAATATA+GGG + chr4_2:50899461-50899480 None:intergenic 30.0%
AAATCCGGTGCATTAAATAT+AGG + chr4_2:50899462-50899481 None:intergenic 30.0%
!!! GTCTGCTTTTTGATATATTG+AGG - chr4_2:50899612-50899631 Msa0605140:intron 30.0%
! TAGATAGATGAACATTTTCC+AGG - chr4_2:50900306-50900325 Msa0605140:intron 30.0%
! AGATAGATGAACATTTTCCA+GGG - chr4_2:50900307-50900326 Msa0605140:intron 30.0%
CTCTCCACAATAAATAACTA+AGG + chr4_2:50900595-50900614 None:intergenic 30.0%
CCCTTATTAATTGTAGTTAG+TGG - chr4_2:50900765-50900784 Msa0605140:five_prime_UTR 30.0%
GTTGAATGATGTGACGAAAA+TGG + chr4_2:50898098-50898117 None:intergenic 35.0%
AAGGTCAATGCAAGTAATGT+TGG + chr4_2:50898205-50898224 None:intergenic 35.0%
TTTGAACAGCAACATGAGAA+AGG + chr4_2:50898224-50898243 None:intergenic 35.0%
GCAAAATCGTTTACTTTCTC+AGG - chr4_2:50898248-50898267 Msa0605140:CDS 35.0%
TTACTTTCTCAGGGATTCAA+AGG - chr4_2:50898258-50898277 Msa0605140:CDS 35.0%
GAAAATGACAGTTACCAAAC+AGG - chr4_2:50898540-50898559 Msa0605140:intron 35.0%
CATTACTCTACAAGTCAGTT+TGG + chr4_2:50898735-50898754 None:intergenic 35.0%
!! CAAACTGACTTGTAGAGTAA+TGG - chr4_2:50898733-50898752 Msa0605140:intron 35.0%
AGGACTTATAGTCTAATGTG+AGG - chr4_2:50898783-50898802 Msa0605140:intron 35.0%
TCAAAACACTCATCGTCAAA+AGG + chr4_2:50899054-50899073 None:intergenic 35.0%
!!! TGACGATGAGTGTTTTGATT+TGG - chr4_2:50899056-50899075 Msa0605140:intron 35.0%
TCTCAAGCTTAAGAACTGTA+GGG + chr4_2:50899172-50899191 None:intergenic 35.0%
TTTGAAGGATGTATTGTTCG+TGG - chr4_2:50899224-50899243 Msa0605140:intron 35.0%
! TATCTCAATAACTTTTGGCC+AGG - chr4_2:50899344-50899363 Msa0605140:CDS 35.0%
AAGAGTTCATTCTTCAAACC+TGG + chr4_2:50899365-50899384 None:intergenic 35.0%
AGAACATTAGAATCAAGGCA+GGG + chr4_2:50899409-50899428 None:intergenic 35.0%
AAGAACATTAGAATCAAGGC+AGG + chr4_2:50899410-50899429 None:intergenic 35.0%
! AATGTGATATCAGGGGATTT+TGG + chr4_2:50899672-50899691 None:intergenic 35.0%
GGCAAAGATTTCTTCAATGA+AGG - chr4_2:50899778-50899797 Msa0605140:intron 35.0%
GTTACTGGATTTATAAGTGG+AGG - chr4_2:50899840-50899859 Msa0605140:intron 35.0%
! TTTCTTCCTTTTCTACCTTC+TGG - chr4_2:50900072-50900091 Msa0605140:intron 35.0%
AAACAACTTGAGCAAAACAC+AGG + chr4_2:50900182-50900201 None:intergenic 35.0%
ACTTCAATCTTCTTAACAGG+TGG + chr4_2:50900358-50900377 None:intergenic 35.0%
TCATGGAACAGATTAAGATG+TGG + chr4_2:50900382-50900401 None:intergenic 35.0%
ACATCTTAATCTGTTCCATG+AGG - chr4_2:50900381-50900400 Msa0605140:CDS 35.0%
GACACTTAAAAAGGAACAGA+GGG + chr4_2:50900489-50900508 None:intergenic 35.0%
TGACACTTAAAAAGGAACAG+AGG + chr4_2:50900490-50900509 None:intergenic 35.0%
GACTATAATTTAGAGACGGA+GGG + chr4_2:50900663-50900682 None:intergenic 35.0%
! AGTCGTTTAAGGTTTGTACA+CGG - chr4_2:50900679-50900698 Msa0605140:intron 35.0%
AGGTTTGTACACGGTTATTA+AGG - chr4_2:50900688-50900707 Msa0605140:CDS 35.0%
! GCAATTATCACCGTTTTGTC+CGG - chr4_2:50897828-50897847 Msa0605140:three_prime_UTR 40.0%
CGTACCTTAATCCTTCTTAC+TGG - chr4_2:50897930-50897949 Msa0605140:three_prime_UTR 40.0%
GGAAACTCACAAATGTGTGA+TGG - chr4_2:50898159-50898178 Msa0605140:exon 40.0%
! TGTGATGGCTTGTATAACGA+TGG - chr4_2:50898174-50898193 Msa0605140:CDS 40.0%
ACGACTAAAACAGACCTGTT+TGG + chr4_2:50898557-50898576 None:intergenic 40.0%
AAGTTATCTTGCAGTCTGCT+TGG + chr4_2:50898631-50898650 None:intergenic 40.0%
GATGATTTACTTGGGGGAAT+TGG - chr4_2:50898840-50898859 Msa0605140:intron 40.0%
GGTTATCGAGAAAGGAACTA+TGG - chr4_2:50899077-50899096 Msa0605140:intron 40.0%
CTCTCAAGCTTAAGAACTGT+AGG + chr4_2:50899173-50899192 None:intergenic 40.0%
GATGTATTGTTCGTGGATAG+TGG - chr4_2:50899231-50899250 Msa0605140:intron 40.0%
ATGACCTTGGACATAGTTTC+GGG + chr4_2:50899276-50899295 None:intergenic 40.0%
CATGACCTTGGACATAGTTT+CGG + chr4_2:50899277-50899296 None:intergenic 40.0%
TTTCAATGTGGACATGACCT+TGG + chr4_2:50899289-50899308 None:intergenic 40.0%
AAGGTCATGTCCACATTGAA+AGG - chr4_2:50899288-50899307 Msa0605140:CDS 40.0%
!! CACATTGAAAGGTGTTCACA+GGG - chr4_2:50899299-50899318 Msa0605140:CDS 40.0%
!!! CACAGGGTTTTGAAACCAAA+TGG - chr4_2:50899315-50899334 Msa0605140:CDS 40.0%
! TCTGTTGAGTGGACAACTTT+TGG - chr4_2:50899489-50899508 Msa0605140:intron 40.0%
AAAGAGCCAGAAGGTAGAAA+AGG + chr4_2:50900081-50900100 None:intergenic 40.0%
!!! TTCTACCTTCTGGCTCTTTT+TGG - chr4_2:50900082-50900101 Msa0605140:intron 40.0%
GTAACACAACTCTCCATCAA+AGG + chr4_2:50900157-50900176 None:intergenic 40.0%
!!! TCTAGTTTTGGTTAGGGAAG+AGG - chr4_2:50900205-50900224 Msa0605140:intron 40.0%
!!! CTAGTTTTGGTTAGGGAAGA+GGG - chr4_2:50900206-50900225 Msa0605140:intron 40.0%
!!! TAGTTTTGGTTAGGGAAGAG+GGG - chr4_2:50900207-50900226 Msa0605140:intron 40.0%
GGCACTTCAATCTTCTTAAC+AGG + chr4_2:50900361-50900380 None:intergenic 40.0%
CTTCACTTTCTAACTCCTCA+TGG + chr4_2:50900399-50900418 None:intergenic 40.0%
CGACTATAATTTAGAGACGG+AGG + chr4_2:50900664-50900683 None:intergenic 40.0%
! ATTATCACCGTTTTGTCCGG+CGG - chr4_2:50897831-50897850 Msa0605140:three_prime_UTR 45.0%
CTAGGGTTGGAAGTCAACAA+TGG - chr4_2:50897856-50897875 Msa0605140:three_prime_UTR 45.0%
TTCTCTCTTTCAGGGATGAG+AGG - chr4_2:50898031-50898050 Msa0605140:three_prime_UTR 45.0%
CGAAGGAAGAGAAATACGAG+TGG - chr4_2:50898058-50898077 Msa0605140:three_prime_UTR 45.0%
AGAGGAATTGGGTGTGAGAT+GGG + chr4_2:50898121-50898140 None:intergenic 45.0%
AGCTCGAGTACAGAGGAATT+GGG + chr4_2:50898132-50898151 None:intergenic 45.0%
AAGCTCGAGTACAGAGGAAT+TGG + chr4_2:50898133-50898152 None:intergenic 45.0%
AATTCCTCTGTACTCGAGCT+TGG - chr4_2:50898132-50898151 Msa0605140:three_prime_UTR 45.0%
!! GTAGCGAATGAGGCTTTGTT+TGG + chr4_2:50898713-50898732 None:intergenic 45.0%
!! AGTCAGTTTGGTAGCGAATG+AGG + chr4_2:50898723-50898742 None:intergenic 45.0%
!! ACTTGGGGGAATTGGTGTTT+TGG - chr4_2:50898848-50898867 Msa0605140:intron 45.0%
!! TTTCGGGCTTTGGATTCCAT+GGG + chr4_2:50899260-50899279 None:intergenic 45.0%
TTGGACATAGTTTCGGGCTT+TGG + chr4_2:50899270-50899289 None:intergenic 45.0%
AAAGCCCGAAACTATGTCCA+AGG - chr4_2:50899269-50899288 Msa0605140:CDS 45.0%
CCTGTGAACACCTTTCAATG+TGG + chr4_2:50899301-50899320 None:intergenic 45.0%
!! CCACATTGAAAGGTGTTCAC+AGG - chr4_2:50899298-50899317 Msa0605140:CDS 45.0%
ATTCATGCCAGCCACATTTC+AGG - chr4_2:50899433-50899452 Msa0605140:intron 45.0%
! AATATAGGGCGCCTGAAATG+TGG + chr4_2:50899447-50899466 None:intergenic 45.0%
GCGCCCTATATTTAATGCAC+CGG - chr4_2:50899455-50899474 Msa0605140:intron 45.0%
TTAATGCACCGGATTTCAGC+TGG - chr4_2:50899466-50899485 Msa0605140:intron 45.0%
TCAACAGACCAGCTGAAATC+CGG + chr4_2:50899477-50899496 None:intergenic 45.0%
! ATTTCAGCTGGTCTGTTGAG+TGG - chr4_2:50899478-50899497 Msa0605140:intron 45.0%
!! TATTGAGGTGCTGCTACCAA+AGG - chr4_2:50899627-50899646 Msa0605140:intron 45.0%
AACACATACGTTCGAGCCTT+TGG + chr4_2:50899646-50899665 None:intergenic 45.0%
CGGTCACTGTAGTACAAAAC+TGG + chr4_2:50899728-50899747 None:intergenic 45.0%
AAGCTCCAAAAAGAGCCAGA+AGG + chr4_2:50900090-50900109 None:intergenic 45.0%
!!! TTTTTGGAGCTTGCTACACC+TGG - chr4_2:50900098-50900117 Msa0605140:intron 45.0%
AGGGGGATTCATCCAATCTT+TGG - chr4_2:50900122-50900141 Msa0605140:intron 45.0%
ACACAACTCTCCATCAAAGG+CGG + chr4_2:50900154-50900173 None:intergenic 45.0%
CAACTTGAGCAAAACACAGG+TGG + chr4_2:50900179-50900198 None:intergenic 45.0%
GGGTATGCACGAGATTGATA+AGG - chr4_2:50900227-50900246 Msa0605140:intron 45.0%
! TGAACATTTTCCAGGGACAG+AGG - chr4_2:50900314-50900333 Msa0605140:intron 45.0%
!! TTAAAAAAAAAAAAAAAAAG+AGG + chr4_2:50899569-50899588 None:intergenic 5.0%
TGTAGCACCCTCAACTGCAT+CGG - chr4_2:50897907-50897926 Msa0605140:three_prime_UTR 50.0%
GAGGAATTGGGTGTGAGATG+GGG + chr4_2:50898120-50898139 None:intergenic 50.0%
CAGAGGAATTGGGTGTGAGA+TGG + chr4_2:50898122-50898141 None:intergenic 50.0%
ACATCCAAGCTCGAGTACAG+AGG + chr4_2:50898139-50898158 None:intergenic 50.0%
TCTGTACTCGAGCTTGGATG+TGG - chr4_2:50898138-50898157 Msa0605140:three_prime_UTR 50.0%
!! GTTTCGGGCTTTGGATTCCA+TGG + chr4_2:50899261-50899280 None:intergenic 50.0%
ATTGGATGAATCCCCCTTCC+AGG + chr4_2:50900119-50900138 None:intergenic 50.0%
CACAACTCTCCATCAAAGGC+GGG + chr4_2:50900153-50900172 None:intergenic 50.0%
CGAAACACACCCGACGACAA+CGG - chr4_2:50897880-50897899 Msa0605140:three_prime_UTR 55.0%
CTACAGTTGCCGTTGTCGTC+GGG + chr4_2:50897892-50897911 None:intergenic 55.0%
GCTACAGTTGCCGTTGTCGT+CGG + chr4_2:50897893-50897912 None:intergenic 55.0%
AGGTACGCCGATGCAGTTGA+GGG + chr4_2:50897917-50897936 None:intergenic 55.0%
AAGGTACGCCGATGCAGTTG+AGG + chr4_2:50897918-50897937 None:intergenic 55.0%
CAGGGATGAGAGGTTCACGA+AGG - chr4_2:50898041-50898060 Msa0605140:three_prime_UTR 55.0%
! TCGTGGATAGTGGTGACCCA+TGG - chr4_2:50899241-50899260 Msa0605140:intron 55.0%
!! TGGAGCTTGCTACACCTGGA+AGG - chr4_2:50900102-50900121 Msa0605140:intron 55.0%
!! GGAGCTTGCTACACCTGGAA+GGG - chr4_2:50900103-50900122 Msa0605140:intron 55.0%
!! GAGCTTGCTACACCTGGAAG+GGG - chr4_2:50900104-50900123 Msa0605140:intron 55.0%
!! AGCTTGCTACACCTGGAAGG+GGG - chr4_2:50900105-50900124 Msa0605140:intron 55.0%
AGGCGGGTAGAGCCAAAGAT+TGG + chr4_2:50900137-50900156 None:intergenic 55.0%
!! TTGGCTCTACCCGCCTTTGA+TGG - chr4_2:50900141-50900160 Msa0605140:intron 55.0%
CATGTGATGACCTCTGTCCC+TGG + chr4_2:50900327-50900346 None:intergenic 55.0%
TGTCCAACTGAGTGCAGGCG+TGG - chr4_2:50897806-50897825 Msa0605140:three_prime_UTR 60.0%
TTGCCACGCCTGCACTCAGT+TGG + chr4_2:50897812-50897831 None:intergenic 60.0%
! TAGGGCGCCTGAAATGTGGC+TGG + chr4_2:50899443-50899462 None:intergenic 60.0%
CCTAGCGCCGCCGGACAAAA+CGG + chr4_2:50897841-50897860 None:intergenic 65.0%
! CGTTTTGTCCGGCGGCGCTA+GGG - chr4_2:50897839-50897858 Msa0605140:three_prime_UTR 65.0%
ACTTCCAACCCTAGCGCCGC+CGG + chr4_2:50897850-50897869 None:intergenic 65.0%
! CCGTTTTGTCCGGCGGCGCT+AGG - chr4_2:50897838-50897857 Msa0605140:three_prime_UTR 70.0%
TTGTCCGGCGGCGCTAGGGT+TGG - chr4_2:50897843-50897862 Msa0605140:three_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 50897802 50900831 50897802 ID=Msa0605140;Name=Msa0605140
chr4_2 mRNA 50897802 50900831 50897802 ID=Msa0605140-mRNA-1;Parent=Msa0605140;Name=Msa0605140-mRNA-1;_AED=0.10;_eAED=0.10;_QI=73|1|1|1|1|1|6|372|255
chr4_2 exon 50897802 50898305 50897802 ID=Msa0605140-mRNA-1:exon:12087;Parent=Msa0605140-mRNA-1
chr4_2 exon 50899085 50899189 50899085 ID=Msa0605140-mRNA-1:exon:12086;Parent=Msa0605140-mRNA-1
chr4_2 exon 50899268 50899402 50899268 ID=Msa0605140-mRNA-1:exon:12085;Parent=Msa0605140-mRNA-1
chr4_2 exon 50899535 50899617 50899535 ID=Msa0605140-mRNA-1:exon:12084;Parent=Msa0605140-mRNA-1
chr4_2 exon 50900354 50900589 50900354 ID=Msa0605140-mRNA-1:exon:12083;Parent=Msa0605140-mRNA-1
chr4_2 exon 50900682 50900831 50900682 ID=Msa0605140-mRNA-1:exon:12082;Parent=Msa0605140-mRNA-1
chr4_2 five_prime_UTR 50900759 50900831 50900759 ID=Msa0605140-mRNA-1:five_prime_utr;Parent=Msa0605140-mRNA-1
chr4_2 CDS 50900682 50900758 50900682 ID=Msa0605140-mRNA-1:cds;Parent=Msa0605140-mRNA-1
chr4_2 CDS 50900354 50900589 50900354 ID=Msa0605140-mRNA-1:cds;Parent=Msa0605140-mRNA-1
chr4_2 CDS 50899535 50899617 50899535 ID=Msa0605140-mRNA-1:cds;Parent=Msa0605140-mRNA-1
chr4_2 CDS 50899268 50899402 50899268 ID=Msa0605140-mRNA-1:cds;Parent=Msa0605140-mRNA-1
chr4_2 CDS 50899085 50899189 50899085 ID=Msa0605140-mRNA-1:cds;Parent=Msa0605140-mRNA-1
chr4_2 CDS 50898174 50898305 50898174 ID=Msa0605140-mRNA-1:cds;Parent=Msa0605140-mRNA-1
chr4_2 three_prime_UTR 50897802 50898173 50897802 ID=Msa0605140-mRNA-1:three_prime_utr;Parent=Msa0605140-mRNA-1
Gene Sequence

>Msa0605140

ATGGACGAAACACACCCGACGACAACGGCAACTGTAGCACCCTCAACTGCATCGGCGTACCTTAATCCTTCTTACTGGGATGAGAGGTTCACGAAGGAAGAGAAATACGAGTGGTTCAAAGATTATTCCCATTTTCGTCACATCATTCAACCCCATCTCACACCCAATTCCTCTGTACTCGAGCTTGGATGTGGAAACTCACAAATGTGTGATGGCTTGTATAACGATGGAACTACCAACATTACTTGCATTGACCTTTCTCATGTTGCTGTTCAAAACATGCAAAATCGTTTACTTTCTCAGGGATTCAAAGACATAAAAGTGATGCAAGCTGATATGCTAGAGCTACCTTTTGACGATGAGTGTTTTGATTTGGTTATCGAGAAAGGAACTATGGATGTATTGTTCGTGGATAGTGGTGACCCATGGAATCCAAAGCCCGAAACTATGTCCAAGGTCATGTCCACATTGAAAGGTGTTCACAGGGTTTTGAAACCAAATGGCATATTTATCTCAATAACTTTTGGCCAGCCACATTTCAGGCGCCCTATATTTAATGCACCGGATTTCAGCTGGTCTGTTGAGTGGACAACTTTTGGTGAAACTTTTCACTATTTTGTGTATGTTCTAAAGAAGGGACAGAGGTCATCACATGAAGATATACCACCTGTTAAGAAGATTGAAGTGCCACATCTTAATCTGTTCCATGAGGAGTTAGAAAGTGAAGATTTTGCTTTTCGAATCAATGTTGATGAGTTGAACTCTTAG
Protein sequence

>Msa0605140

MDETHPTTTATVAPSTASAYLNPSYWDERFTKEEKYEWFKDYSHFRHIIQPHLTPNSSVLELGCGNSQMCDGLYNDGTTNITCIDLSHVAVQNMQNRLLSQGFKDIKVMQADMLELPFDDECFDLVIEKGTMDVLFVDSGDPWNPKPETMSKVMSTLKGVHRVLKPNGIFISITFGQPHFRRPIFNAPDFSWSVEWTTFGETFHYFVYVLKKGQRSSHEDIPPVKKIEVPHLNLFHEELESEDFAFRINVDELNS*