AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0609490

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0609490 MtrunA17_Chr4g0042141 93.548 124 6 1 1 124 1 122 1.39e-81 234
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 70 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAAGTTCCATTGAAGGATT+TGG 0.262218 4_2:+56842668 None:intergenic
TTCGAATCTATGAAGAGATT+TGG 0.340535 4_2:+56842633 None:intergenic
CCGGATTGGAGGCCTTCGTT+GGG 0.381706 4_2:-56842410 Msa0609490:CDS
TGAAGGTAGGTGGCTAGTTC+CGG 0.383752 4_2:-56842492 Msa0609490:CDS
GCCTTCGTTGGGGTCGATAT+CGG 0.394917 4_2:-56842399 Msa0609490:CDS
GTGCTCGTGGTGGTGCTGGT+AGG 0.397018 4_2:-56842310 Msa0609490:CDS
AAATGCACGGTGGCTTCTGC+TGG 0.405731 4_2:-56842356 Msa0609490:CDS
ATCATTTCCAAATCCTTCAA+TGG 0.413195 4_2:-56842675 Msa0609490:five_prime_UTR
GGAAGGTTGGTGCGCCGGAT+TGG 0.415044 4_2:-56842424 Msa0609490:CDS
GCCACAGCAACAACGGGAGC+CGG 0.420519 4_2:+56842473 None:intergenic
ACGGAAGCCACAGCAACAAC+GGG 0.431180 4_2:+56842467 None:intergenic
GCCGGATTGGAGGCCTTCGT+TGG 0.439790 4_2:-56842411 Msa0609490:CDS
CACGTCGGAATCTTTAGTTC+CGG 0.441249 4_2:-56842522 Msa0609490:CDS
GATAACCTTCGCTGTAACTC+CGG 0.444405 4_2:+56842279 None:intergenic
AGTGGTGCTCGTGGTGGTGC+TGG 0.453757 4_2:-56842314 Msa0609490:CDS
GTGGCTTCTGCTGGAAGGGA+GGG 0.455674 4_2:-56842347 Msa0609490:CDS
TCACAAATTTAAAACATGAA+TGG 0.460670 4_2:+56841292 None:intergenic
TCATTCTTCGGAGCCGGATG+AGG 0.461122 4_2:-56842586 Msa0609490:exon
ATTTAAAACATGAATGGCGT+TGG 0.467156 4_2:+56841298 None:intergenic
CCCAACGAAGGCCTCCAATC+CGG 0.470447 4_2:+56842410 None:intergenic
AGGTTGGTGCGCCGGATTGG+AGG 0.471997 4_2:-56842421 Msa0609490:CDS
ATGAAGAGATTTGGCGGTGG+AGG 0.476270 4_2:+56842642 None:intergenic
TGCTCGTGGTGGTGCTGGTA+GGG 0.480254 4_2:-56842309 Msa0609490:CDS
CACGGTGGCTTCTGCTGGAA+GGG 0.487031 4_2:-56842351 Msa0609490:CDS
GGTGGCTTCTGCTGGAAGGG+AGG 0.495597 4_2:-56842348 Msa0609490:CDS
TCCGGCTCCCGTTGTTGCTG+TGG 0.501608 4_2:-56842474 Msa0609490:CDS
AGGGAAATAAGTTCCATTGA+AGG 0.502430 4_2:+56842662 None:intergenic
TGGCTTCTGCTGGAAGGGAG+GGG 0.511927 4_2:-56842346 Msa0609490:CDS
CACGGAAGCCACAGCAACAA+CGG 0.514609 4_2:+56842466 None:intergenic
ATTACTTTAATTTAATTACA+AGG 0.521233 4_2:+56841205 None:intergenic
TTAGTTCCGGCGGAGGATGA+AGG 0.522461 4_2:-56842509 Msa0609490:CDS
AGTTACAGCGAAGGTTATCA+CGG 0.523326 4_2:-56842275 Msa0609490:CDS
GGAATCTTTAGTTCCGGCGG+AGG 0.524866 4_2:-56842516 Msa0609490:CDS
TAAGAGGAAGGTTGGTGCGC+CGG 0.525975 4_2:-56842429 Msa0609490:CDS
AAAACGGTGGAGAGAGGGAA+AGG 0.531145 4_2:+56842608 None:intergenic
TGAGGCGTCAAAATCGTAAG+AGG 0.531246 4_2:-56842445 Msa0609490:CDS
CGCATCGCCGCGGCACACGT+CGG 0.548898 4_2:-56842537 Msa0609490:CDS
TGAAGAGATTTGGCGGTGGA+GGG 0.553533 4_2:+56842643 None:intergenic
GCACGGTGGCTTCTGCTGGA+AGG 0.554007 4_2:-56842352 Msa0609490:CDS
CAAAATCGTAAGAGGAAGGT+TGG 0.559795 4_2:-56842437 Msa0609490:CDS
GAATGAAAACGGTGGAGAGA+GGG 0.560861 4_2:+56842603 None:intergenic
CACCTACCTTCATCCTCCGC+CGG 0.561228 4_2:+56842503 None:intergenic
CCGGCTCCGAAGAATGAAAA+CGG 0.562190 4_2:+56842592 None:intergenic
AGGGAGGGGAAGAAGAAGAG+TGG 0.568872 4_2:-56842332 Msa0609490:CDS
GAATCTATGAAGAGATTTGG+CGG 0.578566 4_2:+56842636 None:intergenic
GCTAGGTGAAACTGTTGCTA+AGG 0.597624 4_2:-56841233 Msa0609490:three_prime_UTR
AGAATGAAAACGGTGGAGAG+AGG 0.606074 4_2:+56842602 None:intergenic
GTCGGAATCTTTAGTTCCGG+CGG 0.609641 4_2:-56842519 Msa0609490:CDS
TCCGATATCGACCCCAACGA+AGG 0.613627 4_2:+56842398 None:intergenic
GTTACAGCGAAGGTTATCAC+GGG 0.620207 4_2:-56842274 Msa0609490:intron
TTTCTTGTTGCGCATCGCCG+CGG 0.624650 4_2:-56842547 Msa0609490:CDS
TATCGTACCGCAGAAATGCA+CGG 0.624676 4_2:-56842369 Msa0609490:CDS
AAAGATTCCGACGTGTGCCG+CGG 0.627143 4_2:+56842530 None:intergenic
CGGATTGGAGGCCTTCGTTG+GGG 0.628555 4_2:-56842409 Msa0609490:CDS
AATGGCGTTGGAGAGAATGC+TGG 0.629506 4_2:+56841310 None:intergenic
GTGCTGGTAGGGTTTATCAC+CGG 0.634833 4_2:-56842298 Msa0609490:CDS
GCGTCAAAATCGTAAGAGGA+AGG 0.639456 4_2:-56842441 Msa0609490:CDS
CGGCGGAGGATGAAGGTAGG+TGG 0.643672 4_2:-56842502 Msa0609490:CDS
AAGAAGAAGAGTGGTGCTCG+TGG 0.645513 4_2:-56842323 Msa0609490:CDS
TTCGTTGGGGTCGATATCGG+AGG 0.655426 4_2:-56842396 Msa0609490:CDS
ATATATGCTAGTATGCTGCT+AGG 0.664065 4_2:-56841250 Msa0609490:three_prime_UTR
ACTTTAATTTAATTACAAGG+TGG 0.668387 4_2:+56841208 None:intergenic
GAAGCCACCGTGCATTTCTG+CGG 0.672142 4_2:+56842362 None:intergenic
TTCCGGCGGAGGATGAAGGT+AGG 0.674632 4_2:-56842505 Msa0609490:CDS
TCTATGAAGAGATTTGGCGG+TGG 0.695619 4_2:+56842639 None:intergenic
GCTCCGAAGAATGAAAACGG+TGG 0.707320 4_2:+56842595 None:intergenic
AAGAAGAGTGGTGCTCGTGG+TGG 0.707624 4_2:-56842320 Msa0609490:CDS
TATCACCGGAGTTACAGCGA+AGG 0.715111 4_2:-56842284 Msa0609490:CDS
TTGTTGCTGTGGCTTCCGTG+AGG 0.746599 4_2:-56842463 Msa0609490:CDS
CGTACCGCAGAAATGCACGG+TGG 0.837388 4_2:-56842366 Msa0609490:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTCAAGAATTATATAAATA+AGG - chr4_2:56841656-56841675 Msa0609490:intron 10.0%
!!! TATTTTCTTTCATTAATTAT+AGG + chr4_2:56841918-56841937 None:intergenic 10.0%
!!! AAATAAAATGAAAGTATTTT+AGG - chr4_2:56842009-56842028 Msa0609490:intron 10.0%
!!! ATTACTTTAATTTAATTACA+AGG + chr4_2:56842652-56842671 None:intergenic 10.0%
!! ATTCAAGAATTATATAAATA+AGG - chr4_2:56841656-56841675 Msa0609490:intron 10.0%
!!! TATTTTCTTTCATTAATTAT+AGG + chr4_2:56841918-56841937 None:intergenic 10.0%
!!! AAATAAAATGAAAGTATTTT+AGG - chr4_2:56842009-56842028 Msa0609490:intron 10.0%
!!! ATTACTTTAATTTAATTACA+AGG + chr4_2:56842652-56842671 None:intergenic 10.0%
!! TAATGAAAGAAAATATGATA+TGG - chr4_2:56841923-56841942 Msa0609490:intron 15.0%
!! AATGAAAGAAAATATGATAT+GGG - chr4_2:56841924-56841943 Msa0609490:intron 15.0%
!! TAATGAAAGAAAATATGATA+TGG - chr4_2:56841923-56841942 Msa0609490:intron 15.0%
!! AATGAAAGAAAATATGATAT+GGG - chr4_2:56841924-56841943 Msa0609490:intron 15.0%
!! AACTTATAAGTCTTCTTATT+AGG - chr4_2:56841870-56841889 Msa0609490:intron 20.0%
!! AAAAAATCAATCAAATGATG+TGG - chr4_2:56842188-56842207 Msa0609490:intron 20.0%
!!! GAATTGAACTTTTTTTTTGA+AGG + chr4_2:56842238-56842257 None:intergenic 20.0%
!! CATAAACAACTATTCAATAT+TGG + chr4_2:56842334-56842353 None:intergenic 20.0%
!!! TATTGTCTTTTGTTTTTGTT+TGG - chr4_2:56842358-56842377 Msa0609490:CDS 20.0%
!!! ATTGTCTTTTGTTTTTGTTT+GGG - chr4_2:56842359-56842378 Msa0609490:CDS 20.0%
!!! TCACAAATTTAAAACATGAA+TGG + chr4_2:56842565-56842584 None:intergenic 20.0%
!!! ACTTTAATTTAATTACAAGG+TGG + chr4_2:56842649-56842668 None:intergenic 20.0%
!! AACTTATAAGTCTTCTTATT+AGG - chr4_2:56841870-56841889 Msa0609490:intron 20.0%
!! AAAAAATCAATCAAATGATG+TGG - chr4_2:56842188-56842207 Msa0609490:intron 20.0%
!!! GAATTGAACTTTTTTTTTGA+AGG + chr4_2:56842238-56842257 None:intergenic 20.0%
!! CATAAACAACTATTCAATAT+TGG + chr4_2:56842334-56842353 None:intergenic 20.0%
!!! TATTGTCTTTTGTTTTTGTT+TGG - chr4_2:56842358-56842377 Msa0609490:CDS 20.0%
!!! ATTGTCTTTTGTTTTTGTTT+GGG - chr4_2:56842359-56842378 Msa0609490:CDS 20.0%
!!! TCACAAATTTAAAACATGAA+TGG + chr4_2:56842565-56842584 None:intergenic 20.0%
!!! ACTTTAATTTAATTACAAGG+TGG + chr4_2:56842649-56842668 None:intergenic 20.0%
!! TGATTTTGTTGAGAATTTAG+GGG + chr4_2:56841135-56841154 None:intergenic 25.0%
!! GTGATTTTGTTGAGAATTTA+GGG + chr4_2:56841136-56841155 None:intergenic 25.0%
!! TGTGATTTTGTTGAGAATTT+AGG + chr4_2:56841137-56841156 None:intergenic 25.0%
!!! ACCTAGTTTTTGAGTTTTAA+CGG - chr4_2:56841617-56841636 Msa0609490:intron 25.0%
!!! TTTTGAGTTTTAACGGATTT+AGG - chr4_2:56841624-56841643 Msa0609490:intron 25.0%
!! AATCATTTTCTGATCAAGTT+TGG - chr4_2:56841744-56841763 Msa0609490:intron 25.0%
! CTTCAACAACAAAACAAATA+AGG - chr4_2:56842117-56842136 Msa0609490:intron 25.0%
! ACAACAAAACAAATAAGGAT+AGG - chr4_2:56842122-56842141 Msa0609490:intron 25.0%
! AAAAAAGTTCAATTCGTACT+AGG - chr4_2:56842243-56842262 Msa0609490:intron 25.0%
!! TTACTTTGTCATTTGTTAAG+TGG - chr4_2:56842286-56842305 Msa0609490:CDS 25.0%
!!! GATTTTTGGTATAAAATTGC+AGG - chr4_2:56842497-56842516 Msa0609490:CDS 25.0%
! CTCAAAACAAAACAAAAAAG+TGG + chr4_2:56842681-56842700 None:intergenic 25.0%
!! TGATTTTGTTGAGAATTTAG+GGG + chr4_2:56841135-56841154 None:intergenic 25.0%
!! GTGATTTTGTTGAGAATTTA+GGG + chr4_2:56841136-56841155 None:intergenic 25.0%
!! TGTGATTTTGTTGAGAATTT+AGG + chr4_2:56841137-56841156 None:intergenic 25.0%
!!! ACCTAGTTTTTGAGTTTTAA+CGG - chr4_2:56841617-56841636 Msa0609490:intron 25.0%
!!! TTTTGAGTTTTAACGGATTT+AGG - chr4_2:56841624-56841643 Msa0609490:intron 25.0%
!! AATCATTTTCTGATCAAGTT+TGG - chr4_2:56841744-56841763 Msa0609490:intron 25.0%
! CTTCAACAACAAAACAAATA+AGG - chr4_2:56842117-56842136 Msa0609490:intron 25.0%
! ACAACAAAACAAATAAGGAT+AGG - chr4_2:56842122-56842141 Msa0609490:intron 25.0%
! AAAAAAGTTCAATTCGTACT+AGG - chr4_2:56842243-56842262 Msa0609490:intron 25.0%
!! TTACTTTGTCATTTGTTAAG+TGG - chr4_2:56842286-56842305 Msa0609490:CDS 25.0%
!!! GATTTTTGGTATAAAATTGC+AGG - chr4_2:56842497-56842516 Msa0609490:CDS 25.0%
! CTCAAAACAAAACAAAAAAG+TGG + chr4_2:56842681-56842700 None:intergenic 25.0%
GGAAATGATGAGAGAAAAAA+TGG + chr4_2:56841168-56841187 None:intergenic 30.0%
ATCATTTCCAAATCCTTCAA+TGG - chr4_2:56841179-56841198 Msa0609490:three_prime_UTR 30.0%
ATAAGTTCCATTGAAGGATT+TGG + chr4_2:56841189-56841208 None:intergenic 30.0%
TTCGAATCTATGAAGAGATT+TGG + chr4_2:56841224-56841243 None:intergenic 30.0%
TCCGTTAAAACTCAAAAACT+AGG + chr4_2:56841621-56841640 None:intergenic 30.0%
! GTATGTTTTGTACTATTGCA+TGG + chr4_2:56841950-56841969 None:intergenic 30.0%
TGCAATAGTACAAAACATAC+TGG - chr4_2:56841950-56841969 Msa0609490:intron 30.0%
CGTGTATACTTTCTATTGAT+AGG + chr4_2:56842091-56842110 None:intergenic 30.0%
! TCACTAACAGACTTGATTTT+TGG - chr4_2:56842483-56842502 Msa0609490:CDS 30.0%
! ATTTAAAACATGAATGGCGT+TGG + chr4_2:56842559-56842578 None:intergenic 30.0%
ACAAAACAAAAAAGTGGTAG+AGG + chr4_2:56842675-56842694 None:intergenic 30.0%
! GTTGATTTAGAAGCAAAAAG+TGG + chr4_2:56842708-56842727 Msa0609500:CDS 30.0%
GGAAATGATGAGAGAAAAAA+TGG + chr4_2:56841168-56841187 None:intergenic 30.0%
ATCATTTCCAAATCCTTCAA+TGG - chr4_2:56841179-56841198 Msa0609490:three_prime_UTR 30.0%
ATAAGTTCCATTGAAGGATT+TGG + chr4_2:56841189-56841208 None:intergenic 30.0%
TTCGAATCTATGAAGAGATT+TGG + chr4_2:56841224-56841243 None:intergenic 30.0%
TCCGTTAAAACTCAAAAACT+AGG + chr4_2:56841621-56841640 None:intergenic 30.0%
! GTATGTTTTGTACTATTGCA+TGG + chr4_2:56841950-56841969 None:intergenic 30.0%
TGCAATAGTACAAAACATAC+TGG - chr4_2:56841950-56841969 Msa0609490:intron 30.0%
CGTGTATACTTTCTATTGAT+AGG + chr4_2:56842091-56842110 None:intergenic 30.0%
! TCACTAACAGACTTGATTTT+TGG - chr4_2:56842483-56842502 Msa0609490:CDS 30.0%
! ATTTAAAACATGAATGGCGT+TGG + chr4_2:56842559-56842578 None:intergenic 30.0%
ACAAAACAAAAAAGTGGTAG+AGG + chr4_2:56842675-56842694 None:intergenic 30.0%
! GTTGATTTAGAAGCAAAAAG+TGG + chr4_2:56842708-56842727 Msa0609500:CDS 30.0%
AGGGAAATAAGTTCCATTGA+AGG + chr4_2:56841195-56841214 None:intergenic 35.0%
GAATCTATGAAGAGATTTGG+CGG + chr4_2:56841221-56841240 None:intergenic 35.0%
ATTCCAAGAAAAACCTCATC+CGG + chr4_2:56841284-56841303 None:intergenic 35.0%
! CATTTTCTGATCAAGTTTGG+AGG - chr4_2:56841747-56841766 Msa0609490:intron 35.0%
CATGAAATTACCCGAATGAT+TGG - chr4_2:56841794-56841813 Msa0609490:intron 35.0%
CTAATAACGACCAATCATTC+GGG + chr4_2:56841807-56841826 None:intergenic 35.0%
CCTAATAACGACCAATCATT+CGG + chr4_2:56841808-56841827 None:intergenic 35.0%
ACATCACTATTGCTTCAACT+AGG - chr4_2:56842035-56842054 Msa0609490:intron 35.0%
ATATATGCTAGTATGCTGCT+AGG - chr4_2:56842604-56842623 Msa0609490:five_prime_UTR 35.0%
AGGGAAATAAGTTCCATTGA+AGG + chr4_2:56841195-56841214 None:intergenic 35.0%
GAATCTATGAAGAGATTTGG+CGG + chr4_2:56841221-56841240 None:intergenic 35.0%
ATTCCAAGAAAAACCTCATC+CGG + chr4_2:56841284-56841303 None:intergenic 35.0%
! CATTTTCTGATCAAGTTTGG+AGG - chr4_2:56841747-56841766 Msa0609490:intron 35.0%
CATGAAATTACCCGAATGAT+TGG - chr4_2:56841794-56841813 Msa0609490:intron 35.0%
CTAATAACGACCAATCATTC+GGG + chr4_2:56841807-56841826 None:intergenic 35.0%
CCTAATAACGACCAATCATT+CGG + chr4_2:56841808-56841827 None:intergenic 35.0%
ACATCACTATTGCTTCAACT+AGG - chr4_2:56842035-56842054 Msa0609490:intron 35.0%
ATATATGCTAGTATGCTGCT+AGG - chr4_2:56842604-56842623 Msa0609490:five_prime_UTR 35.0%
! TCTCCACCGTTTTCATTCTT+CGG - chr4_2:56841256-56841275 Msa0609490:three_prime_UTR 40.0%
CAAAATCGTAAGAGGAAGGT+TGG - chr4_2:56841417-56841436 Msa0609490:intron 40.0%
AGTTACAGCGAAGGTTATCA+CGG - chr4_2:56841579-56841598 Msa0609490:intron 40.0%
! CCGAATGATTGGTCGTTATT+AGG - chr4_2:56841805-56841824 Msa0609490:intron 40.0%
GGTCAACTGCATAATGAACA+AGG + chr4_2:56842070-56842089 None:intergenic 40.0%
! TCTCCACCGTTTTCATTCTT+CGG - chr4_2:56841256-56841275 Msa0609490:three_prime_UTR 40.0%
CAAAATCGTAAGAGGAAGGT+TGG - chr4_2:56841417-56841436 Msa0609490:intron 40.0%
AGTTACAGCGAAGGTTATCA+CGG - chr4_2:56841579-56841598 Msa0609490:intron 40.0%
! CCGAATGATTGGTCGTTATT+AGG - chr4_2:56841805-56841824 Msa0609490:intron 40.0%
GGTCAACTGCATAATGAACA+AGG + chr4_2:56842070-56842089 None:intergenic 40.0%
TCTATGAAGAGATTTGGCGG+TGG + chr4_2:56841218-56841237 None:intergenic 45.0%
GAATGAAAACGGTGGAGAGA+GGG + chr4_2:56841254-56841273 None:intergenic 45.0%
AGAATGAAAACGGTGGAGAG+AGG + chr4_2:56841255-56841274 None:intergenic 45.0%
! CACGTCGGAATCTTTAGTTC+CGG - chr4_2:56841332-56841351 Msa0609490:CDS 45.0%
!! CTTACGATTTTGACGCCTCA+CGG + chr4_2:56841409-56841428 None:intergenic 45.0%
! TGAGGCGTCAAAATCGTAAG+AGG - chr4_2:56841409-56841428 Msa0609490:intron 45.0%
GCGTCAAAATCGTAAGAGGA+AGG - chr4_2:56841413-56841432 Msa0609490:intron 45.0%
TATCGTACCGCAGAAATGCA+CGG - chr4_2:56841485-56841504 Msa0609490:intron 45.0%
GATAACCTTCGCTGTAACTC+CGG + chr4_2:56841578-56841597 None:intergenic 45.0%
GTTACAGCGAAGGTTATCAC+GGG - chr4_2:56841580-56841599 Msa0609490:intron 45.0%
GCTAGGTGAAACTGTTGCTA+AGG - chr4_2:56842621-56842640 Msa0609490:five_prime_UTR 45.0%
TCTATGAAGAGATTTGGCGG+TGG + chr4_2:56841218-56841237 None:intergenic 45.0%
GAATGAAAACGGTGGAGAGA+GGG + chr4_2:56841254-56841273 None:intergenic 45.0%
AGAATGAAAACGGTGGAGAG+AGG + chr4_2:56841255-56841274 None:intergenic 45.0%
! CACGTCGGAATCTTTAGTTC+CGG - chr4_2:56841332-56841351 Msa0609490:CDS 45.0%
!! CTTACGATTTTGACGCCTCA+CGG + chr4_2:56841409-56841428 None:intergenic 45.0%
! TGAGGCGTCAAAATCGTAAG+AGG - chr4_2:56841409-56841428 Msa0609490:intron 45.0%
GCGTCAAAATCGTAAGAGGA+AGG - chr4_2:56841413-56841432 Msa0609490:intron 45.0%
TATCGTACCGCAGAAATGCA+CGG - chr4_2:56841485-56841504 Msa0609490:intron 45.0%
GATAACCTTCGCTGTAACTC+CGG + chr4_2:56841578-56841597 None:intergenic 45.0%
GTTACAGCGAAGGTTATCAC+GGG - chr4_2:56841580-56841599 Msa0609490:intron 45.0%
GCTAGGTGAAACTGTTGCTA+AGG - chr4_2:56842621-56842640 Msa0609490:five_prime_UTR 45.0%
TGAAGAGATTTGGCGGTGGA+GGG + chr4_2:56841214-56841233 None:intergenic 50.0%
ATGAAGAGATTTGGCGGTGG+AGG + chr4_2:56841215-56841234 None:intergenic 50.0%
AAAACGGTGGAGAGAGGGAA+AGG + chr4_2:56841249-56841268 None:intergenic 50.0%
GCTCCGAAGAATGAAAACGG+TGG + chr4_2:56841262-56841281 None:intergenic 50.0%
CCGGCTCCGAAGAATGAAAA+CGG + chr4_2:56841265-56841284 None:intergenic 50.0%
! CCGTTTTCATTCTTCGGAGC+CGG - chr4_2:56841262-56841281 Msa0609490:three_prime_UTR 50.0%
! GAGCCGGATGAGGTTTTTCT+TGG - chr4_2:56841278-56841297 Msa0609490:three_prime_UTR 50.0%
! GTCGGAATCTTTAGTTCCGG+CGG - chr4_2:56841335-56841354 Msa0609490:CDS 50.0%
! TGAAGGTAGGTGGCTAGTTC+CGG - chr4_2:56841362-56841381 Msa0609490:intron 50.0%
AAGAAGAAGAGTGGTGCTCG+TGG - chr4_2:56841531-56841550 Msa0609490:intron 50.0%
! GTGCTGGTAGGGTTTATCAC+CGG - chr4_2:56841556-56841575 Msa0609490:intron 50.0%
TATCACCGGAGTTACAGCGA+AGG - chr4_2:56841570-56841589 Msa0609490:intron 50.0%
AAGTGGAGCTGAGCTATCGT+TGG - chr4_2:56842303-56842322 Msa0609490:CDS 50.0%
! AATGGCGTTGGAGAGAATGC+TGG + chr4_2:56842547-56842566 None:intergenic 50.0%
TGAAGAGATTTGGCGGTGGA+GGG + chr4_2:56841214-56841233 None:intergenic 50.0%
ATGAAGAGATTTGGCGGTGG+AGG + chr4_2:56841215-56841234 None:intergenic 50.0%
AAAACGGTGGAGAGAGGGAA+AGG + chr4_2:56841249-56841268 None:intergenic 50.0%
GCTCCGAAGAATGAAAACGG+TGG + chr4_2:56841262-56841281 None:intergenic 50.0%
CCGGCTCCGAAGAATGAAAA+CGG + chr4_2:56841265-56841284 None:intergenic 50.0%
! CCGTTTTCATTCTTCGGAGC+CGG - chr4_2:56841262-56841281 Msa0609490:three_prime_UTR 50.0%
! GAGCCGGATGAGGTTTTTCT+TGG - chr4_2:56841278-56841297 Msa0609490:three_prime_UTR 50.0%
! GTCGGAATCTTTAGTTCCGG+CGG - chr4_2:56841335-56841354 Msa0609490:CDS 50.0%
! TGAAGGTAGGTGGCTAGTTC+CGG - chr4_2:56841362-56841381 Msa0609490:intron 50.0%
AAGAAGAAGAGTGGTGCTCG+TGG - chr4_2:56841531-56841550 Msa0609490:intron 50.0%
! GTGCTGGTAGGGTTTATCAC+CGG - chr4_2:56841556-56841575 Msa0609490:intron 50.0%
TATCACCGGAGTTACAGCGA+AGG - chr4_2:56841570-56841589 Msa0609490:intron 50.0%
AAGTGGAGCTGAGCTATCGT+TGG - chr4_2:56842303-56842322 Msa0609490:CDS 50.0%
! AATGGCGTTGGAGAGAATGC+TGG + chr4_2:56842547-56842566 None:intergenic 50.0%
TCATTCTTCGGAGCCGGATG+AGG - chr4_2:56841268-56841287 Msa0609490:three_prime_UTR 55.0%
TTTCTTGTTGCGCATCGCCG+CGG - chr4_2:56841307-56841326 Msa0609490:CDS 55.0%
AAAGATTCCGACGTGTGCCG+CGG + chr4_2:56841327-56841346 None:intergenic 55.0%
! GGAATCTTTAGTTCCGGCGG+AGG - chr4_2:56841338-56841357 Msa0609490:CDS 55.0%
TTAGTTCCGGCGGAGGATGA+AGG - chr4_2:56841345-56841364 Msa0609490:intron 55.0%
ACGGAAGCCACAGCAACAAC+GGG + chr4_2:56841390-56841409 None:intergenic 55.0%
CACGGAAGCCACAGCAACAA+CGG + chr4_2:56841391-56841410 None:intergenic 55.0%
TTGTTGCTGTGGCTTCCGTG+AGG - chr4_2:56841391-56841410 Msa0609490:intron 55.0%
!! TAAGAGGAAGGTTGGTGCGC+CGG - chr4_2:56841425-56841444 Msa0609490:intron 55.0%
GCCTTCGTTGGGGTCGATAT+CGG - chr4_2:56841455-56841474 Msa0609490:intron 55.0%
TCCGATATCGACCCCAACGA+AGG + chr4_2:56841459-56841478 None:intergenic 55.0%
TTCGTTGGGGTCGATATCGG+AGG - chr4_2:56841458-56841477 Msa0609490:intron 55.0%
GAAGCCACCGTGCATTTCTG+CGG + chr4_2:56841495-56841514 None:intergenic 55.0%
AAATGCACGGTGGCTTCTGC+TGG - chr4_2:56841498-56841517 Msa0609490:intron 55.0%
AGGGAGGGGAAGAAGAAGAG+TGG - chr4_2:56841522-56841541 Msa0609490:intron 55.0%
AAGAAGAGTGGTGCTCGTGG+TGG - chr4_2:56841534-56841553 Msa0609490:intron 55.0%
TCATTCTTCGGAGCCGGATG+AGG - chr4_2:56841268-56841287 Msa0609490:three_prime_UTR 55.0%
TTTCTTGTTGCGCATCGCCG+CGG - chr4_2:56841307-56841326 Msa0609490:CDS 55.0%
AAAGATTCCGACGTGTGCCG+CGG + chr4_2:56841327-56841346 None:intergenic 55.0%
! GGAATCTTTAGTTCCGGCGG+AGG - chr4_2:56841338-56841357 Msa0609490:CDS 55.0%
TTAGTTCCGGCGGAGGATGA+AGG - chr4_2:56841345-56841364 Msa0609490:intron 55.0%
ACGGAAGCCACAGCAACAAC+GGG + chr4_2:56841390-56841409 None:intergenic 55.0%
CACGGAAGCCACAGCAACAA+CGG + chr4_2:56841391-56841410 None:intergenic 55.0%
TTGTTGCTGTGGCTTCCGTG+AGG - chr4_2:56841391-56841410 Msa0609490:intron 55.0%
!! TAAGAGGAAGGTTGGTGCGC+CGG - chr4_2:56841425-56841444 Msa0609490:intron 55.0%
GCCTTCGTTGGGGTCGATAT+CGG - chr4_2:56841455-56841474 Msa0609490:intron 55.0%
TCCGATATCGACCCCAACGA+AGG + chr4_2:56841459-56841478 None:intergenic 55.0%
TTCGTTGGGGTCGATATCGG+AGG - chr4_2:56841458-56841477 Msa0609490:intron 55.0%
GAAGCCACCGTGCATTTCTG+CGG + chr4_2:56841495-56841514 None:intergenic 55.0%
AAATGCACGGTGGCTTCTGC+TGG - chr4_2:56841498-56841517 Msa0609490:intron 55.0%
AGGGAGGGGAAGAAGAAGAG+TGG - chr4_2:56841522-56841541 Msa0609490:intron 55.0%
AAGAAGAGTGGTGCTCGTGG+TGG - chr4_2:56841534-56841553 Msa0609490:intron 55.0%
TTCCGGCGGAGGATGAAGGT+AGG - chr4_2:56841349-56841368 Msa0609490:intron 60.0%
CACCTACCTTCATCCTCCGC+CGG + chr4_2:56841354-56841373 None:intergenic 60.0%
CCCAACGAAGGCCTCCAATC+CGG + chr4_2:56841447-56841466 None:intergenic 60.0%
! CCGGATTGGAGGCCTTCGTT+GGG - chr4_2:56841444-56841463 Msa0609490:intron 60.0%
! CGGATTGGAGGCCTTCGTTG+GGG - chr4_2:56841445-56841464 Msa0609490:intron 60.0%
CGTACCGCAGAAATGCACGG+TGG - chr4_2:56841488-56841507 Msa0609490:intron 60.0%
CACGGTGGCTTCTGCTGGAA+GGG - chr4_2:56841503-56841522 Msa0609490:intron 60.0%
GTGGCTTCTGCTGGAAGGGA+GGG - chr4_2:56841507-56841526 Msa0609490:intron 60.0%
TGGCTTCTGCTGGAAGGGAG+GGG - chr4_2:56841508-56841527 Msa0609490:intron 60.0%
!! TGCTCGTGGTGGTGCTGGTA+GGG - chr4_2:56841545-56841564 Msa0609490:intron 60.0%
TTCCGGCGGAGGATGAAGGT+AGG - chr4_2:56841349-56841368 Msa0609490:intron 60.0%
CACCTACCTTCATCCTCCGC+CGG + chr4_2:56841354-56841373 None:intergenic 60.0%
CCCAACGAAGGCCTCCAATC+CGG + chr4_2:56841447-56841466 None:intergenic 60.0%
! CCGGATTGGAGGCCTTCGTT+GGG - chr4_2:56841444-56841463 Msa0609490:intron 60.0%
! CGGATTGGAGGCCTTCGTTG+GGG - chr4_2:56841445-56841464 Msa0609490:intron 60.0%
CGTACCGCAGAAATGCACGG+TGG - chr4_2:56841488-56841507 Msa0609490:intron 60.0%
CACGGTGGCTTCTGCTGGAA+GGG - chr4_2:56841503-56841522 Msa0609490:intron 60.0%
GTGGCTTCTGCTGGAAGGGA+GGG - chr4_2:56841507-56841526 Msa0609490:intron 60.0%
TGGCTTCTGCTGGAAGGGAG+GGG - chr4_2:56841508-56841527 Msa0609490:intron 60.0%
!! TGCTCGTGGTGGTGCTGGTA+GGG - chr4_2:56841545-56841564 Msa0609490:intron 60.0%
CGGCGGAGGATGAAGGTAGG+TGG - chr4_2:56841352-56841371 Msa0609490:intron 65.0%
TCCGGCTCCCGTTGTTGCTG+TGG - chr4_2:56841380-56841399 Msa0609490:intron 65.0%
GCCACAGCAACAACGGGAGC+CGG + chr4_2:56841384-56841403 None:intergenic 65.0%
!! GGAAGGTTGGTGCGCCGGAT+TGG - chr4_2:56841430-56841449 Msa0609490:intron 65.0%
!! AGGTTGGTGCGCCGGATTGG+AGG - chr4_2:56841433-56841452 Msa0609490:intron 65.0%
! GCCGGATTGGAGGCCTTCGT+TGG - chr4_2:56841443-56841462 Msa0609490:intron 65.0%
GCACGGTGGCTTCTGCTGGA+AGG - chr4_2:56841502-56841521 Msa0609490:intron 65.0%
GGTGGCTTCTGCTGGAAGGG+AGG - chr4_2:56841506-56841525 Msa0609490:intron 65.0%
! AGTGGTGCTCGTGGTGGTGC+TGG - chr4_2:56841540-56841559 Msa0609490:intron 65.0%
!! GTGCTCGTGGTGGTGCTGGT+AGG - chr4_2:56841544-56841563 Msa0609490:intron 65.0%
CGGCGGAGGATGAAGGTAGG+TGG - chr4_2:56841352-56841371 Msa0609490:intron 65.0%
TCCGGCTCCCGTTGTTGCTG+TGG - chr4_2:56841380-56841399 Msa0609490:intron 65.0%
GCCACAGCAACAACGGGAGC+CGG + chr4_2:56841384-56841403 None:intergenic 65.0%
!! GGAAGGTTGGTGCGCCGGAT+TGG - chr4_2:56841430-56841449 Msa0609490:intron 65.0%
!! AGGTTGGTGCGCCGGATTGG+AGG - chr4_2:56841433-56841452 Msa0609490:intron 65.0%
! GCCGGATTGGAGGCCTTCGT+TGG - chr4_2:56841443-56841462 Msa0609490:intron 65.0%
GCACGGTGGCTTCTGCTGGA+AGG - chr4_2:56841502-56841521 Msa0609490:intron 65.0%
GGTGGCTTCTGCTGGAAGGG+AGG - chr4_2:56841506-56841525 Msa0609490:intron 65.0%
! AGTGGTGCTCGTGGTGGTGC+TGG - chr4_2:56841540-56841559 Msa0609490:intron 65.0%
!! GTGCTCGTGGTGGTGCTGGT+AGG - chr4_2:56841544-56841563 Msa0609490:intron 65.0%
CGCATCGCCGCGGCACACGT+CGG - chr4_2:56841317-56841336 Msa0609490:CDS 75.0%
CGCATCGCCGCGGCACACGT+CGG - chr4_2:56841317-56841336 Msa0609490:CDS 75.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 56841115 56842761 56841115 ID=Msa0609490;Name=Msa0609490
chr4_2 mRNA 56841115 56842761 56841115 ID=Msa0609490-mRNA-1;Parent=Msa0609490;Name=Msa0609490-mRNA-1;_AED=0.00;_eAED=0.00;_QI=170|1|1|1|1|1|2|185|124
chr4_2 exon 56841115 56841357 56841115 ID=Msa0609490-mRNA-1:exon:14739;Parent=Msa0609490-mRNA-1
chr4_2 exon 56842275 56842761 56842275 ID=Msa0609490-mRNA-1:exon:14738;Parent=Msa0609490-mRNA-1
chr4_2 five_prime_UTR 56842592 56842761 56842592 ID=Msa0609490-mRNA-1:five_prime_utr;Parent=Msa0609490-mRNA-1
chr4_2 CDS 56842275 56842591 56842275 ID=Msa0609490-mRNA-1:cds;Parent=Msa0609490-mRNA-1
chr4_2 CDS 56841300 56841357 56841300 ID=Msa0609490-mRNA-1:cds;Parent=Msa0609490-mRNA-1
chr4_2 three_prime_UTR 56841115 56841299 56841115 ID=Msa0609490-mRNA-1:three_prime_utr;Parent=Msa0609490-mRNA-1
Gene Sequence

>Msa0609490

ATGAGGTTTTTCTTGGAATTCGTTTCTTGTTGCGCATCGCCGCGGCACACGTCGGAATCTTTAGTTCCGGCGGAGGATGAAGGTAGGTGGCTAGTTCCGGCTCCCGTTGTTGCTGTGGCTTCCGTGAGGCGTCAAAATCGTAAGAGGAAGGTTGGTGCGCCGGATTGGAGGCCTTCGTTGGGGTCGATATCGGAGGATGTTATCGTACCGCAGAAATGCACGGTGGCTTCTGCTGGAAGGGAGGGGAAGAAGAAGAGTGGTGCTCGTGGTGGTGCTGGTAGGGTTTATCACCGGAGTTACAGCGAAGGTTATCACGGGTCATCTTCTATGCATATGATTATGCCAGCATTCTCTCCAACGCCATTCATGTTTTAA
Protein sequence

>Msa0609490

MRFFLEFVSCCASPRHTSESLVPAEDEGRWLVPAPVVAVASVRRQNRKRKVGAPDWRPSLGSISEDVIVPQKCTVASAGREGKKKSGARGGAGRVYHRSYSEGYHGSSSMHMIMPAFSPTPFMF*