AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0263230

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0263230 MtrunA17_Chr2g0326001 86.496 274 15 5 1 252 1 274 2.72e-147 414
Msa0263230 MtrunA17_Chr4g0030051 47.601 271 80 13 17 248 18 265 1.76e-50 167
Msa0263230 MtrunA17_Chr3g0127201 69.118 68 15 2 29 90 16 83 2.35e-24 99.4
Msa0263230 MtrunA17_Chr1g0188851 50.549 91 35 4 8 88 7 97 1.91e-21 91.3
Msa0263230 MtrunA17_Chr2g0328021 36.306 157 77 6 8 158 31 170 2.53e-21 90.9
Msa0263230 MtrunA17_Chr8g0346551 50.505 99 29 5 23 110 9 98 3.27e-21 90.1
Msa0263230 MtrunA17_Chr3g0126201 61.972 71 21 2 24 88 35 105 2.65e-20 89.0
Msa0263230 MtrunA17_Chr3g0117601 45.185 135 55 7 11 134 29 155 3.28e-20 88.6
Msa0263230 MtrunA17_Chr4g0010151 43.411 129 45 4 7 120 62 177 4.43e-20 88.6
Msa0263230 MtrunA17_Chr4g0031411 53.261 92 30 4 22 100 57 148 7.32e-20 87.4
Msa0263230 MtrunA17_Chr4g0047001 56.338 71 25 2 42 106 57 127 1.25e-18 84.7
Msa0263230 MtrunA17_Chr8g0367161 44.898 98 45 3 18 109 13 107 4.51e-18 82.8
Msa0263230 MtrunA17_Chr8g0374111 52.874 87 32 4 11 90 1 85 1.27e-17 79.7
Msa0263230 MtrunA17_Chr8g0374251 50.617 81 34 2 37 111 65 145 1.31e-17 81.3
Msa0263230 MtrunA17_Chr1g0177251 66.667 54 12 1 41 88 13 66 4.55e-17 79.3
Msa0263230 MtrunA17_Chr7g0237921 53.425 73 28 2 22 88 58 130 5.92e-17 79.7
Msa0263230 MtrunA17_Chr5g0411951 63.636 55 14 2 36 84 39 93 1.71e-16 78.2
Msa0263230 MtrunA17_Chr7g0223311 60.000 65 20 2 39 97 76 140 2.27e-16 78.2
Msa0263230 MtrunA17_Chr2g0282441 62.069 58 13 3 34 85 22 76 2.41e-16 77.8
Msa0263230 MtrunA17_Chr2g0282051 66.667 57 12 2 38 88 44 99 2.52e-16 77.0
Msa0263230 MtrunA17_Chr3g0092111 56.164 73 25 3 26 92 123 194 3.73e-16 77.8
Msa0263230 MtrunA17_Chr4g0047761 68.421 57 11 2 38 88 70 125 6.02e-16 76.3
Msa0263230 MtrunA17_Chr7g0217581 50.000 84 28 3 21 90 205 288 2.01e-15 75.9
Msa0263230 MtrunA17_Chr8g0341671 56.944 72 24 3 28 92 8 79 3.42e-15 72.8
Msa0263230 MtrunA17_Chr5g0417571 55.072 69 24 3 28 90 127 194 5.70e-15 73.6
Msa0263230 MtrunA17_Chr4g0042481 53.846 65 24 2 29 87 111 175 2.12e-14 72.8
Msa0263230 MtrunA17_Chr4g0061761 62.264 53 14 2 38 84 38 90 2.68e-14 71.6
Msa0263230 MtrunA17_Chr8g0355261 51.429 70 27 3 26 89 105 173 3.38e-14 72.0
Msa0263230 MtrunA17_Chr6g0453321 54.839 62 22 2 35 90 131 192 6.13e-14 71.2
Msa0263230 MtrunA17_Chr7g0254161 54.237 59 21 2 35 87 96 154 8.33e-14 70.9
Msa0263230 MtrunA17_Chr6g0460721 43.750 80 38 3 15 88 65 143 9.63e-14 70.1
Msa0263230 MtrunA17_Chr2g0282511 66.000 50 11 2 39 82 44 93 9.75e-14 70.1
Msa0263230 MtrunA17_Chr2g0308011 68.000 50 10 2 39 82 36 85 1.12e-13 68.6
Msa0263230 MtrunA17_Chr6g0460731 37.097 124 68 4 15 132 72 191 1.57e-13 69.7
Msa0263230 MtrunA17_Chr5g0417551 42.718 103 50 4 8 103 96 196 1.71e-12 66.6
Msa0263230 MtrunA17_Chr2g0283811 54.386 57 20 2 37 87 44 100 4.47e-12 62.8
Msa0263230 MtrunA17_Chr5g0417561 51.351 74 30 2 36 103 115 188 6.11e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0263230 AT5G60200.1 35.217 230 92 8 26 240 33 220 1.25e-29 112
Msa0263230 AT2G28510.1 39.111 225 100 10 38 240 46 255 3.94e-27 107
Msa0263230 AT3G45610.1 32.143 252 106 10 11 239 1 210 2.74e-26 103
Msa0263230 AT4G24060.1 65.152 66 17 2 29 88 42 107 7.53e-22 93.6
Msa0263230 AT3G55370.1 52.632 95 33 4 12 99 52 141 7.85e-21 90.5
Msa0263230 AT3G55370.3 52.632 95 33 4 12 99 97 186 8.71e-21 90.9
Msa0263230 AT3G55370.2 52.632 95 33 4 12 99 52 141 1.02e-20 90.5
Msa0263230 AT3G61850.2 55.294 85 26 3 33 110 55 134 1.06e-19 86.7
Msa0263230 AT3G61850.3 55.294 85 26 3 33 110 55 134 1.06e-19 86.7
Msa0263230 AT3G61850.1 55.294 85 26 3 33 110 67 146 1.12e-19 87.0
Msa0263230 AT3G61850.5 55.294 85 26 3 33 110 55 134 1.35e-19 87.0
Msa0263230 AT3G61850.4 55.294 85 26 3 33 110 67 146 1.56e-19 87.0
Msa0263230 AT2G37590.1 54.054 74 28 2 22 89 71 144 4.38e-19 85.9
Msa0263230 AT5G60850.1 61.111 72 18 3 37 98 48 119 8.83e-19 84.7
Msa0263230 AT5G02460.1 63.934 61 16 2 34 88 89 149 1.14e-18 85.1
Msa0263230 AT4G00940.1 61.667 60 17 1 35 88 63 122 1.20e-17 81.3
Msa0263230 AT4G00940.3 61.667 60 17 1 35 88 63 122 1.20e-17 81.3
Msa0263230 AT4G00940.2 61.667 60 17 1 35 88 63 122 1.20e-17 81.3
Msa0263230 AT1G64620.1 60.656 61 18 1 29 83 38 98 1.30e-17 82.0
Msa0263230 AT5G65590.1 54.878 82 26 4 24 94 21 102 1.54e-17 81.3
Msa0263230 AT1G51700.1 47.368 95 40 4 20 107 16 107 6.21e-17 77.4
Msa0263230 AT2G46590.1 48.000 125 53 4 29 143 59 181 1.08e-16 79.3
Msa0263230 AT1G07640.2 43.678 87 42 2 9 88 45 131 1.14e-16 79.0
Msa0263230 AT3G52440.1 58.730 63 20 1 37 93 22 84 1.14e-16 77.8
Msa0263230 AT2G46590.2 48.000 125 53 4 29 143 71 193 1.18e-16 79.3
Msa0263230 AT3G52440.2 58.730 63 20 1 37 93 41 103 1.23e-16 78.2
Msa0263230 AT1G07640.3 43.678 87 42 2 9 88 53 139 1.34e-16 79.0
Msa0263230 AT2G28810.2 62.500 56 15 2 36 85 73 128 2.45e-16 77.8
Msa0263230 AT2G28810.1 62.500 56 15 2 36 85 90 145 3.23e-16 77.8
Msa0263230 AT1G21340.1 63.158 57 15 2 39 89 37 93 3.74e-16 76.6
Msa0263230 AT5G62940.1 57.377 61 20 2 30 84 63 123 4.49e-16 77.8
Msa0263230 AT1G07640.1 50.667 75 30 2 21 88 1 75 8.08e-16 75.9
Msa0263230 AT1G28310.1 65.455 55 13 2 40 88 27 81 1.77e-15 75.5
Msa0263230 AT1G28310.2 65.455 55 13 2 40 88 51 105 2.27e-15 75.5
Msa0263230 AT5G62430.1 52.000 75 29 3 26 93 39 113 2.48e-15 74.7
Msa0263230 AT5G66940.1 48.913 92 33 5 32 110 25 115 5.23e-15 72.8
Msa0263230 AT5G39660.3 50.000 84 30 4 22 99 124 201 7.30e-15 74.3
Msa0263230 AT5G39660.1 50.000 84 30 4 22 99 124 201 7.30e-15 74.3
Msa0263230 AT5G39660.2 50.000 84 30 4 22 99 124 201 7.30e-15 74.3
Msa0263230 AT3G47500.1 58.065 62 20 2 35 90 105 166 1.27e-14 73.6
Msa0263230 AT4G21050.1 53.226 62 21 2 33 88 19 78 1.33e-14 71.2
Msa0263230 AT3G21270.1 66.038 53 12 2 40 86 29 81 1.85e-14 70.9
Msa0263230 AT3G50410.1 56.667 60 20 2 37 90 27 86 2.28e-14 71.6
Msa0263230 AT4G38000.1 52.308 65 24 3 30 87 31 95 5.64e-14 70.1
Msa0263230 AT1G69570.1 55.932 59 20 2 35 87 127 185 1.12e-13 70.9
Msa0263230 AT1G26790.1 45.946 74 34 2 20 87 91 164 1.84e-13 70.1
Msa0263230 AT1G47655.1 57.895 57 18 2 32 82 21 77 2.86e-13 67.8
Msa0263230 AT4G21080.1 38.095 105 58 2 17 114 1 105 3.47e-13 68.2
Msa0263230 AT2G34140.1 52.542 59 22 2 35 87 53 111 8.71e-13 65.5
Msa0263230 AT1G29160.1 52.542 59 22 2 35 87 57 115 2.31e-12 64.3
Msa0263230 AT4G21040.1 37.143 105 59 2 17 114 1 105 2.08e-11 62.8

Find 76 sgRNAs with CRISPR-Local

Find 160 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCATAAACCATGGATTTA+TGG 0.149923 2_2:+62491947 Msa0263230:CDS
GCTTGTTCTGGTTGAGGTTT+TGG 0.170758 2_2:-62491543 None:intergenic
ATCCATAAATCCATGGTTTA+TGG 0.190178 2_2:-62491949 None:intergenic
TTCCATTACCAACCTCATTT+TGG 0.230948 2_2:+62490477 Msa0263230:five_prime_UTR
ACAGTTAGAATCACTATTTC+AGG 0.245561 2_2:+62492914 Msa0263230:CDS
GCAGTGGAAGGGAACAAATT+AGG 0.265618 2_2:+62492168 Msa0263230:CDS
GATTCATTCATCTTATTGTT+TGG 0.284874 2_2:+62490625 Msa0263230:five_prime_UTR
ATTCCTTAGGGTTCCTCCTT+TGG 0.285324 2_2:-62491673 None:intergenic
ATTAGAAGTGGATTATTTCT+TGG 0.296220 2_2:+62491975 Msa0263230:CDS
GGACAGTTCAAAGCTTGTTC+TGG 0.303186 2_2:-62491555 None:intergenic
GAATGAGGGAGATTGTTAAA+TGG 0.311392 2_2:-62491795 None:intergenic
GTTGTTATAGTAACAGAATT+TGG 0.321643 2_2:-62491598 None:intergenic
AGTGGATTATTTCTTGGAAA+TGG 0.336042 2_2:+62491981 Msa0263230:CDS
ATGTATGGTGTGTATGGAAA+TGG 0.354795 2_2:+62492032 Msa0263230:CDS
CAAGATTTGCAGAAATATCT+TGG 0.375372 2_2:-62491636 None:intergenic
AAAGCAATCATGTGGACAAA+TGG 0.379363 2_2:+62491869 Msa0263230:CDS
CAGTGGAAGGGAACAAATTA+GGG 0.389336 2_2:+62492169 Msa0263230:CDS
AGGTAGGTGGTATATATAAT+AGG 0.393165 2_2:-62490439 None:intergenic
CATTCATCTTATTGTTTGGA+TGG 0.393564 2_2:+62490629 Msa0263230:exon
AATGAGGGAGATTGTTAAAT+GGG 0.410826 2_2:-62491794 None:intergenic
TCATCAATGGAAAACATGTT+AGG 0.412061 2_2:+62491492 Msa0263230:CDS
CATCACTCTCTCTTGGGATT+GGG 0.413042 2_2:+62490412 None:intergenic
CCATCACTCTCTCTTGGGAT+TGG 0.433713 2_2:+62490411 None:intergenic
CAGAATATGTATGGTGTGTA+TGG 0.444454 2_2:+62492026 Msa0263230:intron
TTCAAAGCTTGTTCTGGTTG+AGG 0.444926 2_2:-62491549 None:intergenic
CACCAACAGGAACATTCCTT+AGG 0.445109 2_2:-62491686 None:intergenic
TTGTTAAATGGGTTGAGATT+TGG 0.446377 2_2:-62491783 None:intergenic
TGGAAGCAAAGGTGCTGTAT+AGG 0.448996 2_2:-62490459 None:intergenic
TGTATGGTGTGTATGGAAAT+GGG 0.450678 2_2:+62492033 Msa0263230:CDS
AAGCAATCATGTGGACAAAT+GGG 0.452916 2_2:+62491870 Msa0263230:CDS
TAATGCTAATGCAAAATCAT+TGG 0.457564 2_2:-62491835 None:intergenic
TCCTTCTTGTGGACAACCTC+AGG 0.458211 2_2:+62490653 Msa0263230:CDS
GTGTTGGTTGAGTTGCATCT+TGG 0.458480 2_2:-62491576 None:intergenic
AATAATGTTCAGAATATGTA+TGG 0.460682 2_2:+62492017 Msa0263230:intron
ATCCAAAATGAGGTTGGTAA+TGG 0.464507 2_2:-62490479 None:intergenic
TTTCTGCATCCACCACCAAC+AGG 0.469608 2_2:-62491699 None:intergenic
GGGTTTCCATGGCAGCTGAA+TGG 0.475627 2_2:+62492209 Msa0263230:CDS
ATGCAAATAACAATGCTTGT+GGG 0.493320 2_2:+62492084 Msa0263230:CDS
CTCTTTGAAGTTGATGATGA+TGG 0.497955 2_2:-62491738 None:intergenic
GTTCCTCCATTCAGCTGCCA+TGG 0.498909 2_2:-62492215 None:intergenic
AATGTCGAGCCAATCATCAA+TGG 0.508256 2_2:+62491479 Msa0263230:CDS
GACAATGGTGAAGTAAATAG+TGG 0.512707 2_2:+62492056 Msa0263230:CDS
AGGAATGTTCCTGTTGGTGG+TGG 0.512948 2_2:+62491690 Msa0263230:CDS
TTTATGGATGCAATTAGAAG+TGG 0.515360 2_2:+62491963 Msa0263230:CDS
CTAAGGAATGTTCCTGTTGG+TGG 0.527080 2_2:+62491687 Msa0263230:CDS
ATAGTAACAGAATTTGGTGT+TGG 0.529356 2_2:-62491592 None:intergenic
TAATTGCATCCATAAATCCA+TGG 0.534717 2_2:-62491956 None:intergenic
GTTTGGATGGATCCTTCTTG+TGG 0.537133 2_2:+62490642 Msa0263230:exon
ACCAACAGGAACATTCCTTA+GGG 0.541748 2_2:-62491685 None:intergenic
AATGCAAATAACAATGCTTG+TGG 0.543604 2_2:+62492083 Msa0263230:CDS
GTGCTTGGAGTGAAACTGAA+TGG 0.547945 2_2:-62490581 None:intergenic
TATGATCATAAGATGAATGA+GGG 0.553710 2_2:-62491809 None:intergenic
AGCAAGAAATGTGCAGTGGA+AGG 0.565157 2_2:+62492156 Msa0263230:CDS
TTATGATCATAAGATGAATG+AGG 0.565287 2_2:-62491810 None:intergenic
ATTCTAACTGTTCAGACAAG+AGG 0.568973 2_2:-62492902 None:intergenic
ACCTGAGGTTGTCCACAAGA+AGG 0.573402 2_2:-62490654 None:intergenic
ACCCTAAGGAATGTTCCTGT+TGG 0.579058 2_2:+62491684 Msa0263230:CDS
TGCAAATAACAATGCTTGTG+GGG 0.582187 2_2:+62492085 Msa0263230:CDS
TGTAGAAGGTATTGGACCAA+AGG 0.585230 2_2:+62491657 Msa0263230:intron
TGCTTGGAGTGAAACTGAAT+GGG 0.586484 2_2:-62490580 None:intergenic
GGACCAAAGGAGGAACCCTA+AGG 0.594931 2_2:+62491670 Msa0263230:CDS
GAGGTTGGTAATGGAAGCAA+AGG 0.595868 2_2:-62490470 None:intergenic
ATCATCATCAACTTCAAAGA+GGG 0.595973 2_2:+62491740 Msa0263230:CDS
GCAAGAAATGTGCAGTGGAA+GGG 0.598256 2_2:+62492157 Msa0263230:CDS
GTGTATGGAAATGGGGACAA+TGG 0.598902 2_2:+62492041 Msa0263230:CDS
GCTGAATGGAGGAACAAACA+TGG 0.605382 2_2:+62492223 Msa0263230:CDS
AATCATTCAACCATAAACCA+TGG 0.606118 2_2:+62491939 Msa0263230:CDS
CATCATCATCAACTTCAAAG+AGG 0.613529 2_2:+62491739 Msa0263230:CDS
TTTCCATGGCAGCTGAATGG+AGG 0.614048 2_2:+62492212 Msa0263230:CDS
AGAAGGTATTGGACCAAAGG+AGG 0.622045 2_2:+62491660 Msa0263230:intron
GTATGGTGTGTATGGAAATG+GGG 0.629075 2_2:+62492034 Msa0263230:CDS
AGCAAAGGTGCTGTATAGGT+AGG 0.632763 2_2:-62490455 None:intergenic
AGGCTTCAAAAGCAATCATG+TGG 0.635965 2_2:+62491861 Msa0263230:CDS
CTGAATGGAGGAACAAACAT+GGG 0.642499 2_2:+62492224 Msa0263230:CDS
AAAGGTGCTGTATAGGTAGG+TGG 0.642546 2_2:-62490452 None:intergenic
GTGAAGCAAGAAATGTGCAG+TGG 0.683660 2_2:+62492152 Msa0263230:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAAATTAAAGTACTAAAAT+TGG + chr2_2:62491364-62491383 Msa0263230:intron 10.0%
!!! AGAGACAAAATTTATTTTTT+TGG - chr2_2:62491326-62491345 None:intergenic 15.0%
!!! TTAATTTTAACATTTAGCAA+CGG - chr2_2:62491353-62491372 None:intergenic 15.0%
!! AATTATACACAAAATACAAT+TGG - chr2_2:62491393-62491412 None:intergenic 15.0%
!!! TTGTATTTTGTGTATAATTT+TGG + chr2_2:62491394-62491413 Msa0263230:intron 15.0%
!!! TTGTTAATTTTTTGATCATA+GGG - chr2_2:62492131-62492150 None:intergenic 15.0%
!!! ATTGTTAATTTTTTGATCAT+AGG - chr2_2:62492132-62492151 None:intergenic 15.0%
!!! TAATAGAGATTTGATTTATT+TGG + chr2_2:62492557-62492576 Msa0263230:intron 15.0%
!! CAAAAAAAAAAAAAACTGTT+CGG - chr2_2:62492877-62492896 None:intergenic 15.0%
!!! AATATTATGTTTGAAGTTTG+AGG + chr2_2:62490743-62490762 Msa0263230:intron 20.0%
!!! ATATTATGTTTGAAGTTTGA+GGG + chr2_2:62490744-62490763 Msa0263230:intron 20.0%
!!! CAATATATTTTGTGTAAAGA+TGG - chr2_2:62490839-62490858 None:intergenic 20.0%
!! AATTAGATATATAGAAGACA+TGG - chr2_2:62490957-62490976 None:intergenic 20.0%
!!! TTGCAATTTATGTATTTTGT+TGG - chr2_2:62491063-62491082 None:intergenic 20.0%
!! GAGTAATGTGTATTTATATA+TGG + chr2_2:62491241-62491260 Msa0263230:intron 20.0%
!! AGTTGAATCAAAGTTATAAT+AGG - chr2_2:62491282-62491301 None:intergenic 20.0%
!! AATAATGTTCAGAATATGTA+TGG + chr2_2:62492017-62492036 Msa0263230:intron 20.0%
!! TAGAATCAAAAGCTATATTA+TGG + chr2_2:62492326-62492345 Msa0263230:intron 20.0%
!!! TATATTATGGTTCTAAATTG+AGG + chr2_2:62492339-62492358 Msa0263230:intron 20.0%
!!! AGATTTGATTTATTTGGAAT+TGG + chr2_2:62492563-62492582 Msa0263230:intron 20.0%
!! GAAAATAATACAAACAAACA+AGG - chr2_2:62492606-62492625 None:intergenic 20.0%
! GATTCATTCATCTTATTGTT+TGG + chr2_2:62490625-62490644 Msa0263230:five_prime_UTR 25.0%
! TATACTTAAACTGCTCTTAT+GGG - chr2_2:62490799-62490818 None:intergenic 25.0%
! TTATACTTAAACTGCTCTTA+TGG - chr2_2:62490800-62490819 None:intergenic 25.0%
!! AATTTATATGTCTTCTAGCT+TGG + chr2_2:62490973-62490992 Msa0263230:intron 25.0%
! ACAGTATAAAAGATGAAGAT+AGG - chr2_2:62491030-62491049 None:intergenic 25.0%
!! TTTTATTAGATTTGCACTCA+TGG + chr2_2:62491434-62491453 Msa0263230:intron 25.0%
! GTTGTTATAGTAACAGAATT+TGG - chr2_2:62491601-62491620 None:intergenic 25.0%
! TATGATCATAAGATGAATGA+GGG - chr2_2:62491812-62491831 None:intergenic 25.0%
! TTATGATCATAAGATGAATG+AGG - chr2_2:62491813-62491832 None:intergenic 25.0%
! TAATGCTAATGCAAAATCAT+TGG - chr2_2:62491838-62491857 None:intergenic 25.0%
!!! ATTAGAAGTGGATTATTTCT+TGG + chr2_2:62491975-62491994 Msa0263230:CDS 25.0%
!! ATTAGGGAAAGTAAAGTTTT+TGG + chr2_2:62492185-62492204 Msa0263230:CDS 25.0%
!!! TAGCTTTTGATTCTACATTA+GGG - chr2_2:62492321-62492340 None:intergenic 25.0%
!!! ATAGCTTTTGATTCTACATT+AGG - chr2_2:62492322-62492341 None:intergenic 25.0%
!! TCAATTCTCTTAAGTAGTTT+TGG + chr2_2:62492532-62492551 Msa0263230:intron 25.0%
! AAAAAATGTGACATCTTAGA+GGG - chr2_2:62492644-62492663 None:intergenic 25.0%
AGGTAGGTGGTATATATAAT+AGG - chr2_2:62490442-62490461 None:intergenic 30.0%
TGAAAAATCCAAAATGAGGT+TGG - chr2_2:62490488-62490507 None:intergenic 30.0%
AGAGTGAAAAATCCAAAATG+AGG - chr2_2:62490492-62490511 None:intergenic 30.0%
TGGGAAAAAAGAAGAAGAAA+GGG - chr2_2:62490564-62490583 None:intergenic 30.0%
ATGGGAAAAAAGAAGAAGAA+AGG - chr2_2:62490565-62490584 None:intergenic 30.0%
CATTCATCTTATTGTTTGGA+TGG + chr2_2:62490629-62490648 Msa0263230:exon 30.0%
TAGCTTGGAAATTGAAGTTA+GGG + chr2_2:62490988-62491007 Msa0263230:intron 30.0%
! GAAGGTATATACTAGTACTT+AGG - chr2_2:62491097-62491116 None:intergenic 30.0%
!!! AGTAGCACTTTTTTTGAAGA+AGG - chr2_2:62491115-62491134 None:intergenic 30.0%
TACTCACACAACAAAAGTTA+GGG - chr2_2:62491226-62491245 None:intergenic 30.0%
TTACTCACACAACAAAAGTT+AGG - chr2_2:62491227-62491246 None:intergenic 30.0%
!! ACATGTTTTCCATTGATGAT+TGG - chr2_2:62491491-62491510 None:intergenic 30.0%
TCATCAATGGAAAACATGTT+AGG + chr2_2:62491492-62491511 Msa0263230:CDS 30.0%
!! ATAGTAACAGAATTTGGTGT+TGG - chr2_2:62491595-62491614 None:intergenic 30.0%
CAAGATTTGCAGAAATATCT+TGG - chr2_2:62491639-62491658 None:intergenic 30.0%
ATTTCTGCAAATCTTGTAGA+AGG + chr2_2:62491643-62491662 Msa0263230:intron 30.0%
ATCATCATCAACTTCAAAGA+GGG + chr2_2:62491740-62491759 Msa0263230:CDS 30.0%
!! TTGTTAAATGGGTTGAGATT+TGG - chr2_2:62491786-62491805 None:intergenic 30.0%
AATGAGGGAGATTGTTAAAT+GGG - chr2_2:62491797-62491816 None:intergenic 30.0%
! ATTTTGCATTAGCATTAGCT+AGG + chr2_2:62491841-62491860 Msa0263230:CDS 30.0%
AATCATTCAACCATAAACCA+TGG + chr2_2:62491939-62491958 Msa0263230:CDS 30.0%
AACCATAAACCATGGATTTA+TGG + chr2_2:62491947-62491966 Msa0263230:CDS 30.0%
ATCCATAAATCCATGGTTTA+TGG - chr2_2:62491952-62491971 None:intergenic 30.0%
TAATTGCATCCATAAATCCA+TGG - chr2_2:62491959-62491978 None:intergenic 30.0%
TTTATGGATGCAATTAGAAG+TGG + chr2_2:62491963-62491982 Msa0263230:CDS 30.0%
!! AGTGGATTATTTCTTGGAAA+TGG + chr2_2:62491981-62492000 Msa0263230:CDS 30.0%
AATGCAAATAACAATGCTTG+TGG + chr2_2:62492083-62492102 Msa0263230:CDS 30.0%
ATGCAAATAACAATGCTTGT+GGG + chr2_2:62492084-62492103 Msa0263230:CDS 30.0%
!! AGCTTTTGATTCTACATTAG+GGG - chr2_2:62492320-62492339 None:intergenic 30.0%
AAATTGTGTAATTTCCGCAA+AGG - chr2_2:62492446-62492465 None:intergenic 30.0%
TAGAGGGACTAAAAAAAAGA+GGG - chr2_2:62492628-62492647 None:intergenic 30.0%
TTAGAGGGACTAAAAAAAAG+AGG - chr2_2:62492629-62492648 None:intergenic 30.0%
CAAAAAATGTGACATCTTAG+AGG - chr2_2:62492645-62492664 None:intergenic 30.0%
! GAACTATTTTACCTAAAGCA+TGG + chr2_2:62492766-62492785 Msa0263230:intron 30.0%
AAACAAGAAAACCATGCTTT+AGG - chr2_2:62492780-62492799 None:intergenic 30.0%
ACAGTTAGAATCACTATTTC+AGG + chr2_2:62492914-62492933 Msa0263230:CDS 30.0%
! TTCCATTACCAACCTCATTT+TGG + chr2_2:62490477-62490496 Msa0263230:five_prime_UTR 35.0%
!! ATCCAAAATGAGGTTGGTAA+TGG - chr2_2:62490482-62490501 None:intergenic 35.0%
GGGAAAAAAGAAGAAGAAAG+GGG - chr2_2:62490563-62490582 None:intergenic 35.0%
!!! AACTTTGTTTTGTGTGTGCT+TGG - chr2_2:62490599-62490618 None:intergenic 35.0%
GAAGAATATGTTAGCTAGCA+AGG - chr2_2:62490707-62490726 None:intergenic 35.0%
GCTAGCTAACATATTCTTCA+TGG + chr2_2:62490708-62490727 Msa0263230:intron 35.0%
CTAGCTTGGAAATTGAAGTT+AGG + chr2_2:62490987-62491006 Msa0263230:intron 35.0%
!! CTAGTACAAGTTTTCAAGAG+TGG + chr2_2:62491133-62491152 Msa0263230:intron 35.0%
! GCAAATCTTGTAGAAGGTAT+TGG + chr2_2:62491649-62491668 Msa0263230:intron 35.0%
!! CTCTTTGAAGTTGATGATGA+TGG - chr2_2:62491741-62491760 None:intergenic 35.0%
CATCATCATCAACTTCAAAG+AGG + chr2_2:62491739-62491758 Msa0263230:CDS 35.0%
GAATGAGGGAGATTGTTAAA+TGG - chr2_2:62491798-62491817 None:intergenic 35.0%
AAAGCAATCATGTGGACAAA+TGG + chr2_2:62491869-62491888 Msa0263230:CDS 35.0%
AAGCAATCATGTGGACAAAT+GGG + chr2_2:62491870-62491889 Msa0263230:CDS 35.0%
GATCATGATGCTTCAAATCT+AGG + chr2_2:62491900-62491919 Msa0263230:intron 35.0%
GCTTCAAATCTAGGAAATTC+AGG + chr2_2:62491909-62491928 Msa0263230:intron 35.0%
CAGAATATGTATGGTGTGTA+TGG + chr2_2:62492026-62492045 Msa0263230:intron 35.0%
ATGTATGGTGTGTATGGAAA+TGG + chr2_2:62492032-62492051 Msa0263230:CDS 35.0%
TGTATGGTGTGTATGGAAAT+GGG + chr2_2:62492033-62492052 Msa0263230:CDS 35.0%
GACAATGGTGAAGTAAATAG+TGG + chr2_2:62492056-62492075 Msa0263230:CDS 35.0%
TGCAAATAACAATGCTTGTG+GGG + chr2_2:62492085-62492104 Msa0263230:CDS 35.0%
!! AGGGAAAGTAAAGTTTTTGG+TGG + chr2_2:62492188-62492207 Msa0263230:CDS 35.0%
!!! GGGAAAGTAAAGTTTTTGGT+GGG + chr2_2:62492189-62492208 Msa0263230:CDS 35.0%
GTGAAACCATTATTCCAACT+TGG - chr2_2:62492269-62492288 None:intergenic 35.0%
ATAATGGTTTCACATCTCCT+TGG + chr2_2:62492276-62492295 Msa0263230:intron 35.0%
! AGGCATTGTAATCGCAATTA+TGG - chr2_2:62492426-62492445 None:intergenic 35.0%
! CGACTGTCTTGTTGTAATAT+TGG + chr2_2:62492667-62492686 Msa0263230:intron 35.0%
!!! TCCAACTTCATTGATTTTGG+TGG - chr2_2:62492732-62492751 None:intergenic 35.0%
!!! CACTCCAACTTCATTGATTT+TGG - chr2_2:62492735-62492754 None:intergenic 35.0%
! TCTTGTTTGATAGACAGTAG+AGG + chr2_2:62492792-62492811 Msa0263230:intron 35.0%
ACAAGAGAAAACTCAGAATC+AGG - chr2_2:62492854-62492873 None:intergenic 35.0%
ATTCTAACTGTTCAGACAAG+AGG - chr2_2:62492905-62492924 None:intergenic 35.0%
TGCTTGGAGTGAAACTGAAT+GGG - chr2_2:62490583-62490602 None:intergenic 40.0%
AATGTCGAGCCAATCATCAA+TGG + chr2_2:62491479-62491498 Msa0263230:CDS 40.0%
! TTCAAAGCTTGTTCTGGTTG+AGG - chr2_2:62491552-62491571 None:intergenic 40.0%
!! TGTAGAAGGTATTGGACCAA+AGG + chr2_2:62491657-62491676 Msa0263230:intron 40.0%
ACCAACAGGAACATTCCTTA+GGG - chr2_2:62491688-62491707 None:intergenic 40.0%
AGGCTTCAAAAGCAATCATG+TGG + chr2_2:62491861-62491880 Msa0263230:CDS 40.0%
GTATGGTGTGTATGGAAATG+GGG + chr2_2:62492034-62492053 Msa0263230:CDS 40.0%
CAGTGGAAGGGAACAAATTA+GGG + chr2_2:62492169-62492188 Msa0263230:CDS 40.0%
!!! AAGTTTTTGGTGGGTTTCCA+TGG + chr2_2:62492198-62492217 Msa0263230:CDS 40.0%
CTGAATGGAGGAACAAACAT+GGG + chr2_2:62492224-62492243 Msa0263230:CDS 40.0%
TTGAATCAGCAAGACCAAGT+TGG + chr2_2:62492252-62492271 Msa0263230:intron 40.0%
! GCAAGACCAAGTTGGAATAA+TGG + chr2_2:62492260-62492279 Msa0263230:intron 40.0%
GATTCTACATTAGGGGACTA+TGG - chr2_2:62492313-62492332 None:intergenic 40.0%
TTGAGGCTATGTTTACGTTG+CGG + chr2_2:62492356-62492375 Msa0263230:intron 40.0%
TACGTTGCGGATCTTGATAT+TGG + chr2_2:62492369-62492388 Msa0263230:intron 40.0%
ACGTTGCGGATCTTGATATT+GGG + chr2_2:62492370-62492389 Msa0263230:intron 40.0%
TTGCGATTACAATGCCTTTG+CGG + chr2_2:62492429-62492448 Msa0263230:intron 40.0%
TGGTGGCAAACATACATATC+TGG - chr2_2:62492715-62492734 None:intergenic 40.0%
GCCACCAAAATCAATGAAGT+TGG + chr2_2:62492728-62492747 Msa0263230:intron 40.0%
!! AAAGGTGCTGTATAGGTAGG+TGG - chr2_2:62490455-62490474 None:intergenic 45.0%
!! AGCAAAGGTGCTGTATAGGT+AGG - chr2_2:62490458-62490477 None:intergenic 45.0%
!! TGGAAGCAAAGGTGCTGTAT+AGG - chr2_2:62490462-62490481 None:intergenic 45.0%
!! GAGGTTGGTAATGGAAGCAA+AGG - chr2_2:62490473-62490492 None:intergenic 45.0%
GTGCTTGGAGTGAAACTGAA+TGG - chr2_2:62490584-62490603 None:intergenic 45.0%
GTTTGGATGGATCCTTCTTG+TGG + chr2_2:62490642-62490661 Msa0263230:exon 45.0%
CTAATGAGTAGTCCTACCTG+AGG - chr2_2:62490672-62490691 None:intergenic 45.0%
CTCAGGTAGGACTACTCATT+AGG + chr2_2:62490670-62490689 Msa0263230:intron 45.0%
!! GCTTGTTCTGGTTGAGGTTT+TGG - chr2_2:62491546-62491565 None:intergenic 45.0%
! GGACAGTTCAAAGCTTGTTC+TGG - chr2_2:62491558-62491577 None:intergenic 45.0%
!! GTGTTGGTTGAGTTGCATCT+TGG - chr2_2:62491579-62491598 None:intergenic 45.0%
!! AGAAGGTATTGGACCAAAGG+AGG + chr2_2:62491660-62491679 Msa0263230:intron 45.0%
ATTCCTTAGGGTTCCTCCTT+TGG - chr2_2:62491676-62491695 None:intergenic 45.0%
ACCCTAAGGAATGTTCCTGT+TGG + chr2_2:62491684-62491703 Msa0263230:CDS 45.0%
CACCAACAGGAACATTCCTT+AGG - chr2_2:62491689-62491708 None:intergenic 45.0%
CTAAGGAATGTTCCTGTTGG+TGG + chr2_2:62491687-62491706 Msa0263230:CDS 45.0%
GTGTATGGAAATGGGGACAA+TGG + chr2_2:62492041-62492060 Msa0263230:CDS 45.0%
GTGAAGCAAGAAATGTGCAG+TGG + chr2_2:62492152-62492171 Msa0263230:CDS 45.0%
AGCAAGAAATGTGCAGTGGA+AGG + chr2_2:62492156-62492175 Msa0263230:CDS 45.0%
GCAAGAAATGTGCAGTGGAA+GGG + chr2_2:62492157-62492176 Msa0263230:CDS 45.0%
GCAGTGGAAGGGAACAAATT+AGG + chr2_2:62492168-62492187 Msa0263230:CDS 45.0%
GCTGAATGGAGGAACAAACA+TGG + chr2_2:62492223-62492242 Msa0263230:CDS 45.0%
GTTTCACATCTCCTTGGCAA+GGG + chr2_2:62492282-62492301 Msa0263230:intron 45.0%
CGTTGCGGATCTTGATATTG+GGG + chr2_2:62492371-62492390 Msa0263230:intron 45.0%
GGGTAAAATGCAGACAAGTG+TGG + chr2_2:62492391-62492410 Msa0263230:intron 45.0%
TCCTTCTTGTGGACAACCTC+AGG + chr2_2:62490653-62490672 Msa0263230:CDS 50.0%
ACCTGAGGTTGTCCACAAGA+AGG - chr2_2:62490657-62490676 None:intergenic 50.0%
TCTTGTGGACAACCTCAGGT+AGG + chr2_2:62490657-62490676 Msa0263230:intron 50.0%
! AGGAATGTTCCTGTTGGTGG+TGG + chr2_2:62491690-62491709 Msa0263230:CDS 50.0%
TTTCTGCATCCACCACCAAC+AGG - chr2_2:62491702-62491721 None:intergenic 50.0%
TTTCCATGGCAGCTGAATGG+AGG + chr2_2:62492212-62492231 Msa0263230:CDS 50.0%
GGTTTCACATCTCCTTGGCA+AGG + chr2_2:62492281-62492300 Msa0263230:intron 50.0%
GGACCAAAGGAGGAACCCTA+AGG + chr2_2:62491670-62491689 Msa0263230:CDS 55.0%
GGGTTTCCATGGCAGCTGAA+TGG + chr2_2:62492209-62492228 Msa0263230:CDS 55.0%
GTTCCTCCATTCAGCTGCCA+TGG - chr2_2:62492218-62492237 None:intergenic 55.0%
CTATGGAGAAGCCCTTGCCA+AGG - chr2_2:62492296-62492315 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 62490425 62492965 62490425 ID=Msa0263230;Name=Msa0263230
chr2_2 mRNA 62490425 62492965 62490425 ID=Msa0263230-mRNA-1;Parent=Msa0263230;Name=Msa0263230-mRNA-1;_AED=0.21;_eAED=0.21;_QI=223|0.16|0.14|0.85|0.5|0.71|7|0|273
chr2_2 exon 62490425 62490674 62490425 ID=Msa0263230-mRNA-1:exon:19325;Parent=Msa0263230-mRNA-1
chr2_2 exon 62491477 62491624 62491477 ID=Msa0263230-mRNA-1:exon:19326;Parent=Msa0263230-mRNA-1
chr2_2 exon 62491636 62491657 62491636 ID=Msa0263230-mRNA-1:exon:19327;Parent=Msa0263230-mRNA-1
chr2_2 exon 62491665 62491891 62491665 ID=Msa0263230-mRNA-1:exon:19328;Parent=Msa0263230-mRNA-1
chr2_2 exon 62491931 62492022 62491931 ID=Msa0263230-mRNA-1:exon:19329;Parent=Msa0263230-mRNA-1
chr2_2 exon 62492029 62492269 62492029 ID=Msa0263230-mRNA-1:exon:19330;Parent=Msa0263230-mRNA-1
chr2_2 exon 62492901 62492965 62492901 ID=Msa0263230-mRNA-1:exon:19331;Parent=Msa0263230-mRNA-1
chr2_2 five_prime_UTR 62490425 62490647 62490425 ID=Msa0263230-mRNA-1:five_prime_utr;Parent=Msa0263230-mRNA-1
chr2_2 CDS 62490648 62490674 62490648 ID=Msa0263230-mRNA-1:cds;Parent=Msa0263230-mRNA-1
chr2_2 CDS 62491477 62491624 62491477 ID=Msa0263230-mRNA-1:cds;Parent=Msa0263230-mRNA-1
chr2_2 CDS 62491636 62491657 62491636 ID=Msa0263230-mRNA-1:cds;Parent=Msa0263230-mRNA-1
chr2_2 CDS 62491665 62491891 62491665 ID=Msa0263230-mRNA-1:cds;Parent=Msa0263230-mRNA-1
chr2_2 CDS 62491931 62492022 62491931 ID=Msa0263230-mRNA-1:cds;Parent=Msa0263230-mRNA-1
chr2_2 CDS 62492029 62492269 62492029 ID=Msa0263230-mRNA-1:cds;Parent=Msa0263230-mRNA-1
chr2_2 CDS 62492901 62492965 62492901 ID=Msa0263230-mRNA-1:cds;Parent=Msa0263230-mRNA-1
Gene Sequence

>Msa0263230

ATGGATCCTTCTTGTGGACAACCTCAGCAAATGTCGAGCCAATCATCAATGGAAAACATGTTAGGTTGCTCAAAAGATGAGCAAGAAAGAAAGCCAAAACCTCAACCAGAACAAGCTTTGAACTGTCCAAGATGCAACTCAACCAACACCAAATTCTGTTACTATAACAACTATACCAAGATATTTCTGCAAATCTTGTATTGGACCAAAGGAGGAACCCTAAGGAATGTTCCTGTTGGTGGTGGATGCAGAAAAAACAAAAGATCATCACCATCATCATCAACTTCAAAGAGGGTTCAAGATCAAACATTCACACCAAATCTCAACCCATTTAACAATCTCCCTCATTCATCTTATGATCATAATTCCAATGATTTTGCATTAGCATTAGCTAGGCTTCAAAAGCAATCATGTGGACAAATGGGTATGAACAATCATTCAACCATAAACCATGGATTTATGGATGCAATTAGAAGTGGATTATTTCTTGGAAATGGACTACATTATAATAATAATAATATGTATGGTGTGTATGGAAATGGGGACAATGGTGAAGTAAATAGTGGTAACAATGCAAATAACAATGCTTGTGGGGTTAGTGAAGAAATGATGTTTCCCTATGATCAAAAAATTAACAATGTGAAGCAAGAAATGTGCAGTGGAAGGGAACAAATTAGGGAAAGTAAAGTTTTTGGTGGGTTTCCATGGCAGCTGAATGGAGGAACAAACATGGGTGAAGTTGAATCAGCAAGACCAATCCTCTTGTCTGAACAGTTAGAATCACTATTTCAGGAACTTTCAGTTTTGAAATATTCCTGTTGA
Protein sequence

>Msa0263230

MDPSCGQPQQMSSQSSMENMLGCSKDEQERKPKPQPEQALNCPRCNSTNTKFCYYNNYTKIFLQILYWTKGGTLRNVPVGGGCRKNKRSSPSSSTSKRVQDQTFTPNLNPFNNLPHSSYDHNSNDFALALARLQKQSCGQMGMNNHSTINHGFMDAIRSGLFLGNGLHYNNNNMYGVYGNGDNGEVNSGNNANNNACGVSEEMMFPYDQKINNVKQEMCSGREQIRESKVFGGFPWQLNGGTNMGEVESARPILLSEQLESLFQELSVLKYSC*